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Email: don.becker-at-bruker.com Name: Don Becker
Organization: Bruker Nano
Title-Subject: [Filtered] Job Opening at Bruker Nano Analytics
Message: Bruker Nano Analytics has an immediate opening for a Senior Sales Representative for Microanalysis in the Southeastern United States. The successful candidate will have a minimum of 3 -n 5 years experience in the sale of scientific equipment, and will sell, market, and support our microanalysis tools with new and existing customers, as well as support our OEM sales channel partners. The sales territory includes CO, NM, TX, OK, AL, NC, SC, FL, GA, LA, and MS.
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We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.
Laboratory exercises, demonstrations, and discussions include: * geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; * phase contrast, polarization and interference microscopy; * fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; * principles and application of digital imaging and quantitative digital image deconvolution; * digital image processing and object identification and tracking; * live cell and ratiometric imaging for FRET and ion concentration imaging; * confocal microscopy and specialized methods such as TIRF and FLIM; and * new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.
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Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility 9 Cambridge Center, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 12, 39 -- From wsalmon-at-wi.mit.edu Wed Jan 6 15:42:09 2016 12, 39 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 12, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u06Lg8aa011850 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 15:42:08 -0600 12, 39 -- X-IronPort-AV: E=Sophos;i="5.20,530,1444708800"; 12, 39 -- d="scan'208";a="3335172" 12, 39 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 12, 39 -- by ptolemy.wi.mit.edu with ESMTP; 06 Jan 2016 16:41:59 -0500 12, 39 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 2518E31EB48 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:59 -0500 (EST) 12, 39 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 12, 39 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 12, 39 -- with ESMTP id 88pSmo5FAWfT for {microscopy-at-microscopy.com} ; 12, 39 -- Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 9E23231EB62 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 12, 39 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 12, 39 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 12, 39 -- with ESMTP id vkLbfRjMwVqd for {microscopy-at-microscopy.com} ; 12, 39 -- Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 83F4331EAFE 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- Date: Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 12, 39 -- To: microscopy-at-microscopy.com 12, 39 -- Message-ID: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu} 12, 39 -- In-Reply-To: {1538907122.10462611.1452116467905.JavaMail.zimbra-at-wi.mit.edu} 12, 39 -- Subject: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 12, 39 -- MIME-Version: 1.0 12, 39 -- Content-Type: text/plain; charset=utf-8 12, 39 -- Content-Transfer-Encoding: 7bit 12, 39 -- X-Originating-IP: [10.9.4.20] 12, 39 -- X-Mailer: Zimbra 8.0.7_GA_6021 (ZimbraWebClient - GC47 (Mac)/8.0.7_GA_6021) 12, 39 -- Thread-Topic: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 12, 39 -- Thread-Index: 3I4RFXAc6NuHKgHiTFr8kP+ljEXmnA== ==============================End of - Headers==============================
Hello All, We have a trouble with our old MegaViewII TEM camera. Just before the end of the last year, the power source of a PC we are using with MegaViewII camera has gone. Just now we replaced the power source with a new one. All seemed to be OK but when we tried to record the image with MegaViewII camera we got a strange result. The image can be seen on this link:
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 10, 42 -- From benada-at-biomed.cas.cz Thu Jan 7 08:45:03 2016 10, 42 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 10, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u07Ej2X8016084 10, 42 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 08:45:03 -0600 10, 42 -- X-ASG-Debug-ID: 1452177892-05011e5425340270001-4CH8be 10, 42 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id cndEuIYa6CHL3S7G (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 07 Jan 2016 15:44:52 +0100 (CET) 10, 42 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 10, 42 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 10, 42 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 10, 42 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 10, 42 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 10, 42 -- (No client certificate requested) 10, 42 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 4D04B2C8070 10, 42 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 15:44:48 +0100 (CET) 10, 42 -- Date: Thu, 7 Jan 2016 15:44:47 +0100 10, 42 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 42 -- To: {microscopy-at-microscopy.com} 10, 42 -- Subject: MegaViewII Trouble 10, 42 -- Message-ID: {20160107154447.26f75438-at-u117ob02} 10, 42 -- X-ASG-Orig-Subj: MegaViewII Trouble 10, 42 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 42 -- =?UTF-8?B?xIxS?= 10, 42 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 10, 42 -- MIME-Version: 1.0 10, 42 -- Content-Type: text/plain; charset=US-ASCII 10, 42 -- Content-Transfer-Encoding: 7bit 10, 42 -- X-IoP-CAS-MailScanner-ID: 4D04B2C8070.90340 10, 42 -- X-IoP-CAS-MailScanner: Processed 10, 42 -- X-Spam-Status: No 10, 42 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 10, 42 -- X-Barracuda-Start-Time: 1452177892 10, 42 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 10, 42 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 10, 42 -- X-Barracuda-Scan-Msg-Size: 1664 10, 42 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 10, 42 -- X-Barracuda-BRTS-Status: 1 10, 42 -- X-Barracuda-Spam-Score: 0.00 10, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 10, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25917 10, 42 -- Rule breakdown below 10, 42 -- pts rule name description 10, 42 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-lbl.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-lbl.gov Name: Marissa
Organization: LBL
Title-Subject: [Filtered] Glove box
Message: To those of you who transfer samples to a TEM holder in a glove box, will you please contact me offline with the name of the glove box supplier?
Thanks!
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Thu Jan 7 14:55:16 2016 15, 40 -- Received: from mail-qg0-f48.google.com (mail-qg0-f48.google.com [209.85.192.48]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u07KtEBH010432 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 14:55:15 -0600 15, 40 -- Received: by mail-qg0-f48.google.com with SMTP id e32so247793076qgf.3 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 07 Jan 2016 12:55:05 -0800 (PST) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=wzdhJD5Y9ce3fflsfduzcRkZDzpQXc6DhbYciIWEdRk=; 15, 40 -- b=XigH4yoXVV5cMAHme/ZHgnkavbdBQwya05fLDWZkF17f4o27MF80LMPK4uf/9aPcci 15, 40 -- zWzKhPfTcWVpUciLjVyMH9TDRTkzkcxwZ1ROKtZ64kUz1Si9Fc6ZA4hiACcFKNvYjvvF 15, 40 -- 0swWFJi7jZ3Q5CUqurYB1DkVIamxlGos14AxNMq806c4CoLIULHxzpXavMXiACVcUm32 15, 40 -- crVMJJtGv/rFR6kADa3lwLFcjZvwSmOXuzcC567WTl+cO0RclC8hLrPGk3BEb/yYwOO6 15, 40 -- rVoTRp9sDO8cfUZf0hX8jqQotozwfhP6TPM8qulm/MKZUHPf3unGiKTCYq/c5YP/XETe 15, 40 -- H06g== 15, 40 -- X-Received: by 10.140.174.2 with SMTP id u2mr80610201qhu.62.1452200105375; 15, 40 -- Thu, 07 Jan 2016 12:55:05 -0800 (PST) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id y104sm18387621qgd.33.2016.01.07.12.55.04 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 15, 40 -- Thu, 07 Jan 2016 12:55:04 -0800 (PST) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW: TEM Glove box sample transfer 15, 40 -- References: {201601071953.u07JrlLx009792-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201601071953.u07JrlLx009792-at-ns.microscopy.com} 15, 40 -- Message-ID: {568ED0A7.4080400-at-microscopy.com} 15, 40 -- Date: Thu, 7 Jan 2016 14:55:03 -0600 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.5.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201601071953.u07JrlLx009792-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello Warren, Ravi and Larry, Thank you for your comments and suggestions. Yesterday I forgot one important think, the pattern (image) that we are getting from megaViewII does not depend on electron beam intensity. Even when the beam is switched off, we get this pattern (image).
Best regards Oldrich
On Thu, 7 Jan 2016 15:58:40 +0000, "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} wrote : } It looks like the synchronization has gone out. It is like what I see } on our SEM when the raster on the sample doesn't match the raster on } the screen. } } I presume the system is operating in TEM mode and that the MegaView } is a CCD camera. If so, the only way that such a phenomenon might } occur is if the number of pixels per line is not matching between the } CCD and display. It seems the number of pixels across your image is } slightly less than the number being read across the CCD. Therefore, } each successive line is shifted some to the right. } } Is the sample a nice simple structure? I would wonder what a single } vertical grid bar would look like. } } Warren } } -----Original Message----- } From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz] } Sent: Thursday, January 07, 2016 8:48 AM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] MegaViewII Trouble } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } We have a trouble with our old MegaViewII TEM camera. Just before the } end of the last year, the power source of a PC we are using with } MegaViewII camera has gone. Just now we replaced the power source with } a new one. All seemed to be OK but when we tried to record the image } with MegaViewII camera we got a strange result. The image can be seen } on this link: } } http://www2.biomed.cas.cz/~benada/MegaViewII_trouble.html } } } Please does anybody had to solve such problem? } } Thanking for any response in advance. } Oldrich } } -- } Oldrich Benada } Institute of Microbiology CAS, v.v.i. } Videnska 1083 } 142 20 Prague 4 } Czech Republic
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==============================Original Headers============================== 7, 49 -- From benada-at-biomed.cas.cz Fri Jan 8 02:13:04 2016 7, 49 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u088D3op013161 7, 49 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 02:13:04 -0600 7, 49 -- X-ASG-Debug-ID: 1452240773-05011e5425351cb0001-4CH8be 7, 49 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id oykzTSJlFOKTZZTf (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 08 Jan 2016 09:12:53 +0100 (CET) 7, 49 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 7, 49 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 7, 49 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 7, 49 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 7, 49 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 49 -- (No client certificate requested) 7, 49 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 92E1E2C80FA; 7, 49 -- Fri, 8 Jan 2016 09:12:49 +0100 (CET) 7, 49 -- Date: Fri, 8 Jan 2016 09:12:49 +0100 7, 49 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 7, 49 -- To: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} , 7, 49 -- {microscopy-at-microscopy.com} , {les-at-zsgenetics.com} , 7, 49 -- Ravi Thakkar 7, 49 -- {ravi.thakkar369-at-gmail.com} 7, 49 -- Subject: Re: [Microscopy] MegaViewII Trouble 7, 49 -- Message-ID: {20160108091249.40d565b0-at-u117ob02} 7, 49 -- X-ASG-Orig-Subj: Re: [Microscopy] MegaViewII Trouble 7, 49 -- In-Reply-To: {CY1PR04MB1813866E43A724B10C93B3B1D7F50-at-CY1PR04MB1813.namprd04.prod.outlook.com} 7, 49 -- References: {201601071447.u07Elg9u016882-at-ns.microscopy.com} 7, 49 -- {CY1PR04MB1813866E43A724B10C93B3B1D7F50-at-CY1PR04MB1813.namprd04.prod.outlook.com} 7, 49 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 7, 49 -- =?UTF-8?B?xIxS?= 7, 49 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 7, 49 -- MIME-Version: 1.0 7, 49 -- Content-Type: text/plain; charset=US-ASCII 7, 49 -- Content-Transfer-Encoding: 7bit 7, 49 -- X-IoP-CAS-MailScanner-ID: 92E1E2C80FA.98AF0 7, 49 -- X-IoP-CAS-MailScanner: Processed 7, 49 -- X-Spam-Status: No 7, 49 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 7, 49 -- X-Barracuda-Start-Time: 1452240773 7, 49 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 7, 49 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 7, 49 -- X-Barracuda-Scan-Msg-Size: 3452 7, 49 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 7, 49 -- X-Barracuda-BRTS-Status: 1 7, 49 -- X-Barracuda-Spam-Score: 0.00 7, 49 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_SC0_MISMATCH_TO 7, 49 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25946 7, 49 -- Rule breakdown below 7, 49 -- pts rule name description 7, 49 -- ---- ---------------------- -------------------------------------------------- 7, 49 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header ==============================End of - Headers==============================
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Email: gary.bloomer-at-montana.edu Name: Gary Bloomer
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Title-Subject: [Filtered] Chromophores characterized for 2-photon microscopy
Message: We are doing 2-photon microscopy but the available chromophores all seem to be characterized only for 1 photon fluorescence. Setting parameters on the light source according to this data may be creating an error in our results. Are others concerned with this and are there solutions such as look-up tables available to properly set laser parameters for available dyes?
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==============================Original Headers============================== 12, 40 -- From microscopy.listserver-at-gmail.com Fri Jan 8 07:44:15 2016 12, 40 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176]) 12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08DiDcm012228 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 07:44:14 -0600 12, 40 -- Received: by mail-io0-f176.google.com with SMTP id 77so255214254ioc.2 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 08 Jan 2016 05:44:04 -0800 (PST) 12, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 40 -- d=gmail.com; s=20120113; 12, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 12, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 12, 40 -- bh=m38CZ0tYTKgidhCo3hLR/qY8vTCi1OBYk0yJJ/0HiBM=; 12, 40 -- b=DztjP7xQD90BIdzGMR5c3w2b2nq61+L2m9OHkC2SnoX9RtmgHQtW0QjunDQhR2E6X/ 12, 40 -- nSCEPDH1lcce7uA2sgDt1XrFHCpKSCh5q89dluFOGf3D1l0GAxstfPjtMRSraGBn1xik 12, 40 -- WooLx9ou6aEIIFAzH9gq2VtV77ZzRxbP5pq9FJ6Zi9VDiL6oTq8BsW3HNghOq8Bcbtja 12, 40 -- au1EvO6ra0j35HTwo56DXdGcesCOr1PGmEgCpjVQM/6fSnOAc4zIdLVbuPUPJtOLcKEo 12, 40 -- vSjz9n1uAtttqHUZCJ6Nuev52eOhKfpSf/kuI76RDZGM8DcW6nTShh+ixBFWs2DrIryJ 12, 40 -- HwYQ== 12, 40 -- X-Received: by 10.107.9.224 with SMTP id 93mr97073491ioj.112.1452260644386; 12, 40 -- Fri, 08 Jan 2016 05:44:04 -0800 (PST) 12, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 12, 40 -- by smtp.googlemail.com with ESMTPSA id n6sm6735362ige.12.2016.01.08.05.44.03 12, 40 -- for {microscopy-at-microscopy.com} 12, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 12, 40 -- Fri, 08 Jan 2016 05:44:03 -0800 (PST) 12, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 12, 40 -- {microscopylistserver-noreply-at-microscopy.com} 12, 40 -- Subject: viaWWW:Chromophores characterized for 2-photon microscopy 12, 40 -- References: {201601072301.u07N1pve032564-at-ns.microscopy.com} 12, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 40 -- X-Forwarded-Message-Id: {201601072301.u07N1pve032564-at-ns.microscopy.com} 12, 40 -- Message-ID: {568FBD23.1090507-at-microscopy.com} 12, 40 -- Date: Fri, 8 Jan 2016 07:44:03 -0600 12, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 12, 40 -- Gecko/20100101 Thunderbird/38.5.0 12, 40 -- MIME-Version: 1.0 12, 40 -- In-Reply-To: {201601072301.u07N1pve032564-at-ns.microscopy.com} 12, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From hanja07656515151viu-at-gmail.com Fri Jan 8 08:09:14 2016 Return-Path: {hanja07656515151viu-at-gmail.com} Received: from gmail.com (220-134-188-200.HINET-IP.hinet.net [220.134.188.200]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u08E9BX8030857 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 8 Jan 2016 08:09:13 -0600 Message-ID: {C2B18D2B.24421114-at-gmail.com}
Dear Listers,
I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them through a small Millipore filter and then processing this filter disc in the usual way up through CPD, mounting, sputter coating with gold/palladium for SEM. Most of the pili seem to have fallen off the bacteria, plenty of loose pili on the filter beside the bacteria.
Couple of questions for everybody. First, can fixation cause pili to shear off the bacteria? Second, anyone have any suggestions on how to keep the pili attached to the bacteria for SEM observation? Thanks in advance, all help is appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id B5B455C3BF6 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 37 -- (No client certificate requested) 8, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 8, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37 -- Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index: AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 14:26:44 +0000 8, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org} 8, 37 -- Accept-Language: en-US 8, 37 -- Content-Language: en-US 8, 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type: text/plain; charset="us-ascii" 8, 37 -- MIME-Version: 1.0 8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 8, 37 -- X-VPM-CERT-FLAG: 0 8, 37 -- X-VPM-IS-HYBRID: 0 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08EQuO4003928 ==============================End of - Headers==============================
From advertise.bz222uuv-at-gmail.com Fri Jan 8 09:32:23 2016 Return-Path: {advertise.bz222uuv-at-gmail.com} Received: from gmail.com ([1.236.84.191]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u08FWKsx027065 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 8 Jan 2016 09:32:21 -0600 Message-ID: {62EBFA9A.D09DB7CA-at-gmail.com}
Tom, Are you following the primary fixative with osmium? Does this strain have pili? You can run a quick negative stain prep to confirm pili. Also maybe some tannic acid in the primary fixative along with PF and Ga. I never heard of pili falling of during fixation.
Good luck, Michael Delannoy
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Friday, January 08, 2016 9:38 AM To: delannoy-at-jhmi.edu
Dear Listers,
I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them through a small Millipore filter and then processing this filter disc in the usual way up through CPD, mounting, sputter coating with gold/palladium for SEM. Most of the pili seem to have fallen off the bacteria, plenty of loose pili on the filter beside the bacteria.
Couple of questions for everybody. First, can fixation cause pili to shear off the bacteria? Second, anyone have any suggestions on how to keep the pili attached to the bacteria for SEM observation? Thanks in advance, all help is appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id B5B455C3BF6 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 37 -- (No client certificate requested) 8, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 8, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37 -- Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index: AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 14:26:44 +0000 8, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org} 8, 37 -- Accept-Language: en-US 8, 37 -- Content-Language: en-US 8, 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type: text/plain; charset="us-ascii" 8, 37 -- MIME-Version: 1.0 8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 8, 37 -- X-VPM-CERT-FLAG: 0 8, 37 -- X-VPM-IS-HYBRID: 0 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08EQuO4003928 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 24 -- From prvs=808477f31=mdelann1-at-jhmi.edu Fri Jan 8 10:38:14 2016 16, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.130]) 16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08GcE5W027977 16, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 10:38:14 -0600 16, 24 -- X-IronPort-AV: E=Sophos;i="5.20,539,1444708800"; 16, 24 -- d="scan'208";a="204135271" 16, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 16, 24 -- by IPEB1.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 08 Jan 2016 11:38:03 -0500 16, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 16, 24 -- To: {tbargar-at-unmc.edu} 16, 24 -- Cc: {Microscopy-at-microscopy.com} , {microscopy.listserver-at-gmail.com} , 16, 24 -- {microscopy-at-msa.microscopy.com} 16, 24 -- References: {201601081437.u08EbZvM010463-at-ns.microscopy.com} 16, 24 -- In-Reply-To: {201601081437.u08EbZvM010463-at-ns.microscopy.com} 16, 24 -- Subject: RE: [Microscopy] Stabilizing pili on bacteria for SEM 16, 24 -- Date: Fri, 8 Jan 2016 11:38:06 -0500 16, 24 -- Message-ID: {007b01d14a32$f3daad70$db900850$-at-jhmi.edu} 16, 24 -- MIME-Version: 1.0 16, 24 -- Content-Type: text/plain; 16, 24 -- charset="us-ascii" 16, 24 -- Content-Transfer-Encoding: 7bit 16, 24 -- X-Mailer: Microsoft Outlook 14.0 16, 24 -- Thread-Index: AQKcnZohv6HMCmg8szDXNpP2BC84M51bKVGg 16, 24 -- Content-Language: en-us ==============================End of - Headers==============================
Hello Tom, We are using E. coli in our practical courses of electron microscopy in biology. We usually process E. coli samples for TEM and SEM in parallel.
At first, do you really need a SEM for imaging E. coli pili? TEM and negative staining is much more faster a you even do not need to fix E. coli sample at all. In our hand 1% ammonium molybdate, pH6.8-7.0 plus 0.1% trehalose mixture works quite well for negative staining of bacteria on glow-discharge treated formvar/carbon grids.
Our procedure for SEM: 1. Start with 0.5 ml of E. coli culture; optical density ranging from 0.4 to 0.6. 2. Fix it with 3% glutaraldehyde for half an hour at room temperature, then continue the fixation at 4 oC overnight. 3. Wash the fixed cells well with phosphate or cacodylate buffer (three times at least). 4. Make a moist chamber from Petri dish, wet filter paper and Parafilm. 5. Place some poly-l-lysine treated circular cover-slips on the Parafilm in the moist chamber and put 100 ,Aei 200 uL of fixed cells in the washing buffer onto the individual cover-slip. Whole cover-slip area should be covered with liquid. Let sediment the cells onto the cover-slips at 4 oC overnight. 6. Wash the cover-slips with ddH2O. 7. Dehydrate through alcohol series; CPD; ... .
With this procedure the pili should stay attached to bacteria.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm currently fixing a strain of E. Coli with pili with 2%GA , } 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them } through a small Millipore filter and then processing this filter disc } in the usual way up through CPD, mounting, sputter coating with } gold/palladium for SEM. Most of the pili seem to have fallen off the } bacteria, plenty of loose pili on the filter beside the bacteria. } } Couple of questions for everybody. First, can fixation cause pili to } shear off the bacteria? Second, anyone have any suggestions on how } to keep the pili attached to the bacteria for SEM observation? } Thanks in advance, all help is appreciated. } } Tom Bargar } UNMC } Electron Microscopy Core Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } The information in this e-mail may be privileged and confidential, } intended only for the use of the addressee(s) above. Any unauthorized } use or disclosure of this information is prohibited. If you have } received this e-mail by mistake, please delete it and immediately } contact the sender. } } } } ==============================Original } Headers============================== 8, 37 -- From tbargar-at-unmc.edu } Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu } (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, } 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM } [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with
Virginia Tech in Blacksburg, VA, USA has an opening for an Applications Specialist in Scanning and Transmission Electron Microscopy. This position will help support internal and external users of the Virginia Tech National Center for Earth and Environmental Nanotechnology Infrastructure (NCE- {=NI) under the direct supervision of NCE- {=NI Associate Director for Instrumentation & Technical Development. The successful candidate will have their office located within the Nanoscale Characterization and Fabrication Laboratory (NCFL). The NCFL houses advanced field emission scanning electron microscopes with EDS and EBSD capabilities, several transmission electron microscopes with EDS, tomography, HRTEM, cryoTEM, and other capabilities, and a suite of powerful surface spectroscopy tools including magnetic sector SIMS, XPS, and more. Multiple sample preparation laboratories facilitate the preparation of samples for analysis using SEM, TEM, AFM, EBSD, and other techniques.
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To view a more detailed list of expected duties and the qualifications required, visit:
https://listings.jobs.vt.edu/postings/62637
Applicants can apply to this position by visiting the above link and clicking on the "Apply for this Job" link near the top of the page. If the above link does not work, then you can apply by visiting www.jobs.vt.edu and searching for posting number SR0150183.
Virginia Tech does not discriminate against employees, students, or applicants on the basis of age, color, disability, gender, gender identity, gender expression, national origin, political affiliation, race, religion, sexual orientation, genetic information, veteran status, or any other basis protected by law. For inquiries regarding non-discrimination policies, contact the executive director for Equity and Access at 540-231-8771 or Virginia Tech, North End Center, Suite 2300 (0318), 300 Turner St. NW, Blacksburg, VA 24061.
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From improve.alexa.rankslsuzn-at-gmail.com Sat Jan 9 13:10:08 2016 Return-Path: {improve.alexa.rankslsuzn-at-gmail.com} Received: from gmail.com ([211.63.33.142]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u09JA2g5002263 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 9 Jan 2016 13:10:05 -0600 Message-ID: {F8A49867.6949C4DE-at-gmail.com}
Dear Microscopists,
We have an entry level opening in our Tucson manufacturing facility suitable for an enthusiastic energetic recent graduate with good communication skills, to join us as a full time EM tech in our applications support lab.
Initial duties include:-
* Ultramicrotome QC - cut ultra thin sections & evaluate in TEM + basic QC evaluation of sub assemblies/components + submit reports & documentation * Glass Knife Maker test, calibration and QC reports * Rotary microtome QC - cut semi-thin to thick paraffin and materials science samples - evaluate & report * TEM maintenance
with opportunities for growth in the following areas:
* Applications support evaluating customer specimens and advising on appropriate sample prep techniques - includes both life science and materials science disciplines * Cryo Ultramicrotome test and evaluation * Assist with workshops and product demonstrations * Field support if applicant is willing and able to travel * Sales and Service
Please respond by email to info-at-boeckeler.com
-- Dave Roberts Director-RMC Microscopy Products RMC-Boeckeler Boeckeler Instruments Inc 4650 S Butterfield Drive Tucson, Arizona 85714 Tel: 520-745-0001 Fax: 520-745-0004 www.rmcproducts.com Skype:dave.robertsrmc
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Someone has asked me about microscope attachments for iPhone. I've seen lots of ads, but I haven't looked closely at any of the products. Do any of you have opinions about products that turn your iPhone or Android into decent microscopes? I'm not sure if she wants to go with a stand and slides, or just take nicely magnified images on the go. I think the former...
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DIVppj017621 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 -0600 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0DIVVV9025775 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST) 4, 21 -- Received: from localhost (tina-at-localhost) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0DIVUQ2025771; 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 21 -- X-X-Sender: tina-at-b1000 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU 4, 21 -- Subject: iPhone microscope 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000} 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Here at PNNL we,Aeove actually developed a low-cost (~$1) cellphone camera that magnifies up to 1000x, depending on the specification. Our lab has freely released the blueprints for 3D printing or you can order pre-manufactured lenses that clip onto pretty much any smartphone. We use them frequently in our demos to high school and middle school children and they work quite well once you get the hang of them.
Here,Aeos a link to more details: http://availabletechnologies.pnnl.gov/technology.asp?id=393
Feel free to contact me if you have any questions.
* The views and opinions expressed in this email are my own and do not necessarily reflect those of PNNL, the Battelle Memorial Institute, the United States Government, or any agency thereof. ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
Someone has asked me about microscope attachments for iPhone. I've seen lots of ads, but I haven't looked closely at any of the products. Do any of you have opinions about products that turn your iPhone or Android into decent microscopes? I'm not sure if she wants to go with a stand and slides, or just take nicely magnified images on the go. I think the former...
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DIVppj017621 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 -0600 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0DIVVV9025775 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST) 4, 21 -- Received: from localhost (tina-at-localhost) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0DIVUQ2025771; 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 21 -- X-X-Sender: tina-at-b1000 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU 4, 21 -- Subject: iPhone microscope 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000} 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13 13:11:57 2016 22, 29 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov [192.101.109.63]) 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DJBuxL006200 22, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 13:11:56 -0600 22, 29 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212]) 22, 29 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 13 Jan 2016 11:11:45 -0800 22, 29 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.82]) by EX10CASHUB06.pnnl.gov 22, 29 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Wed, 13 Jan 2016 11:11:45 22, 29 -- -0800 22, 29 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} 22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} 22, 29 -- Subject: Re: [Microscopy] iPhone microscope 22, 29 -- Thread-Topic: [Microscopy] iPhone microscope 22, 29 -- Thread-Index: AQHRTjQyIKPRV14ru0iCfvZUlj7Hu5750BIA 22, 29 -- Date: Wed, 13 Jan 2016 19:11:44 +0000 22, 29 -- Message-ID: {062CD028-BFB5-47EE-A028-A5F9044AB27E-at-pnnl.gov} 22, 29 -- References: {201601131856.u0DIudAw004154-at-ns.microscopy.com} 22, 29 -- In-Reply-To: {201601131856.u0DIudAw004154-at-ns.microscopy.com} 22, 29 -- Accept-Language: en-US 22, 29 -- Content-Language: en-US 22, 29 -- X-MS-Has-Attach: 22, 29 -- X-MS-TNEF-Correlator: 22, 29 -- x-originating-ip: [130.20.128.10] 22, 29 -- Content-Type: text/plain; charset="utf-8" 22, 29 -- Content-ID: {D94556B5D077EF4595F2908D97115031-at-pnnl.gov} 22, 29 -- MIME-Version: 1.0 22, 29 -- Content-Transfer-Encoding: 8bit 22, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u0DJBuxL006200 ==============================End of - Headers==============================
Hi Tina, Though not as cheap as the PNNL design, some colleagues of mine have used this inexpensive, hardware-store-materials-based home-built design for outreach and liked it: http://www.instructables.com/id/10-Smartphone-to-digital-microscope-conversion/ Tyler Harvey University of Oregon
On 2016-01-13 11:29, steven.spurgeon-at-pnnl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Tina, } } Here at PNNL we/c,C ,Ncve actually developed a low-cost (~$1) cellphone } camera that magnifies up to 1000x, depending on the specification. Our } lab has freely released the blueprints for 3D printing or you can } order pre-manufactured lenses that clip onto pretty much any } smartphone. We use them frequently in our demos to high school and } middle school children and they work quite well once you get the hang } of them. } } Here/c,C ,Ncs a link to more details: } http://availabletechnologies.pnnl.gov/technology.asp?id=393 } } Feel free to contact me if you have any questions. } } * The views and opinions expressed in this email are my own and do not } necessarily reflect those of PNNL, the Battelle Memorial Institute, } the United States Government, or any agency thereof. } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Physical and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } } } X-from: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} } Reply-To: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} } Date: Wednesday, January 13, 2016 at 10:56 AM } To: Steven Spurgeon {steven.spurgeon-at-pnnl.gov} } Subject: [Microscopy] iPhone microscope } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } My apologies for cross-posting... } } Someone has asked me about microscope attachments for iPhone. I've seen } lots of ads, but I haven't looked closely at any of the products. Do } any } of you have opinions about products that turn your iPhone or Android } into } decent microscopes? I'm not sure if she wants to go with a stand and } slides, or just take nicely magnified images on the go. I think the } former... } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } ==============================Original } Headers============================== } 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016 } 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu } [128.171.22.30]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u0DIVppj017621 } 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 } -0600 } 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) } 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id } u0DIVVV9025775 } 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NO); } 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST) } 4, 21 -- Received: from localhost (tina-at-localhost) } 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP } id u0DIVUQ2025771; } 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST) } 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned } process doing -bs } 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST) } 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 4, 21 -- X-X-Sender: tina-at-b1000 } 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , } 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU } 4, 21 -- Subject: iPhone microscope } 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000} } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13 } 13:11:57 2016 } 22, 29 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov } [192.101.109.63]) } 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id u0DJBuxL006200 } 22, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 13:11:56 } -0600 } 22, 29 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212]) } 22, 29 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 13 Jan } 2016 11:11:45 -0800 } 22, 29 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.82]) by } EX10CASHUB06.pnnl.gov } 22, 29 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Wed, 13 Jan } 2016 11:11:45 } 22, 29 -- -0800 } 22, 29 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , } 22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} } 22, 29 -- Subject: Re: [Microscopy] iPhone microscope } 22, 29 -- Thread-Topic: [Microscopy] iPhone microscope } 22, 29 -- Thread-Index: AQHRTjQyIKPRV14ru0iCfvZUlj7Hu5750BIA } 22, 29 -- Date: Wed, 13 Jan 2016 19:11:44 +0000 } 22, 29 -- Message-ID: {062CD028-BFB5-47EE-A028-A5F9044AB27E-at-pnnl.gov} } 22, 29 -- References: {201601131856.u0DIudAw004154-at-ns.microscopy.com} } 22, 29 -- In-Reply-To: {201601131856.u0DIudAw004154-at-ns.microscopy.com} } 22, 29 -- Accept-Language: en-US } 22, 29 -- Content-Language: en-US } 22, 29 -- X-MS-Has-Attach: } 22, 29 -- X-MS-TNEF-Correlator: } 22, 29 -- x-originating-ip: [130.20.128.10] } 22, 29 -- Content-Type: text/plain; charset="utf-8" } 22, 29 -- Content-ID: {D94556B5D077EF4595F2908D97115031-at-pnnl.gov} } 22, 29 -- MIME-Version: 1.0 } 22, 29 -- Content-Transfer-Encoding: 8bit } 22, 29 -- X-MIME-Autoconverted: from base64 to 8bit by } ns.microscopy.com id u0DJBuxL006200 } ==============================End of - } Headers==============================
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Email: Phil.Ahrenkiel-at-sdsmt.edu Name: Phil Ahrenkiel
Organization: SDSMT
Title-Subject: [Filtered] Orius column defect
Message: Does anybody know how to use the "Defects" box in the "Camera Configuration" dialog in DM for an Orius? I have spent several hours getting remote help from Gatan, but nobody is able to make that feature actually work. The format seems to be "c2098", for column 2098. We are using DM1.85.1535.
The details of our problem are shown here: http://ahrenkiel.sdsmt.edu/JEM2100_TEM/troubleshooting/default.htm
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Title-Subject: [Filtered] Liposome in skin tissue - Ultrastructure by TEM
Message: Does anyone worked on visualizing liposomes in skin tissue by TEM. Is there any special precautions/steps to consider for processing the tissue.?
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Pili are extremely delicate, I would guess that the filtration step is the critical one in your protocol because it requires pressure. In this regard, the protocol of Oldrich is much softer since the cells are sedimented. We placed bacteria on a porous membrane (used for cell biology) and sucked the liquid from the bottom side with a filter. The filters give charging in SEM and they tend to melt under the beam, so be careful with your electrons! (some years ago we had porous membranes made of aluminium, they were perfect for the job but I think they are not produced anymore)
Regards Stephane
-------------------------------------------- On Fri, 1/8/16, benada-at-biomed.cas.cz {benada-at-biomed.cas.cz} wrote:
Subject: [Microscopy] Re: Stabilizing pili on bacteria for SEM To: nizets2-at-yahoo.com Date: Friday, January 8, 2016, 6:13 PM
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Hello Tom, We are using E. coli in our practical courses of electron microscopy in biology. We usually process E. coli samples for TEM and SEM in parallel.
At first, do you really need a SEM for imaging E. coli pili? TEM and negative staining is much more faster a you even do not need to fix E. coli sample at all. In our hand 1% ammonium molybdate, pH6.8-7.0 plus 0.1% trehalose mixture works quite well for negative staining of bacteria on glow-discharge treated formvar/carbon grids.
Our procedure for SEM: 1. Start with 0.5 ml of E. coli culture; optical density ranging from 0.4 to 0.6. 2. Fix it with 3% glutaraldehyde for half an hour at room temperature, then continue the fixation at 4 oC overnight. 3. Wash the fixed cells well with phosphate or cacodylate buffer (three times at least). 4. Make a moist chamber from Petri dish, wet filter paper and Parafilm. 5. Place some poly-l-lysine treated circular cover-slips on the Parafilm in the moist chamber and put 100 ,Aei 200 uL of fixed cells in the washing buffer onto the individual cover-slip. Whole cover-slip area should be covered with liquid. Let sediment the cells onto the cover-slips at 4 oC overnight. 6. Wash the cover-slips with ddH2O. 7. Dehydrate through alcohol series; CPD; ... .
With this procedure the pili should stay attached to bacteria.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:-* The Microscopy Society of } America To-* Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm currently fixing a strain of E. Coli with pili with 2%GA , } 2%Paraform. In .1M Sorensen's phosphate buffer.-* Filtering them } through a small Millipore filter and then processing this filter disc } in the usual way up through CPD, mounting, sputter coating with } gold/palladium for SEM.-* Most of the pili seem to have fallen off the } bacteria, plenty of loose pili on the filter beside the bacteria. } } Couple of questions for everybody.-* First, can fixation cause pili to } shear off the bacteria?-*-*-*Second, anyone have any suggestions on how } to keep the pili attached to the bacteria for SEM observation? } Thanks in advance, all help is appreciated. } } Tom Bargar } UNMC } Electron Microscopy Core Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } The information in this e-mail may be privileged and confidential, } intended only for the use of the addressee(s) above. Any unauthorized } use or disclosure of this information is prohibited. If you have } received this e-mail by mistake, please delete it and immediately } contact the sender. } } } -* } ==============================Original } Headers============================== 8, 37 -- From tbargar-at-unmc.edu } Fri Jan-* 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu } (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- -*-*-* by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u08EQuO4003928 8, 37 -- -*-*-* for {Microscopy-at-microscopy.com} ; Fri, } 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM } [127.0.0.1]) 8, 37 -- -*-*-* by Outbound.unmc.edu (Proprietary) with } SMTP id B5B455C3BF6 8, 37 -- -*-*-* for {Microscopy-at-microscopy.com} ; } Fri,-* 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from } UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37 -- } (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 37 -- } (No client certificate requested) 8, 37 -- -*-*-* by } zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D 8, 37 -- } -*-*-* for {Microscopy-at-microscopy.com} ; Fri,-* 8 Jan 2016 08:07:20 } -0600 (CST) 8, 37 -- Received: from UNMCEX2.unmcresforest.org } ([fe80::151d:89d:49b6:a0ab]) by 8, 37 ---* UNMCEX1.unmcresforest.org } ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- } 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 -- From: } "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 37 -- To: } "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37 -- } Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic: } Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index: } AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 } 14:26:44 +0000 8, 37 -- Message-ID: } {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org} } 8, 37 -- Accept-Language: en-US 8, 37 -- Content-Language: en-US 8, } 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- } x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type: text/plain; } charset="us-ascii" 8, 37 -- MIME-Version: 1.0 } 8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce } 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu } 8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d } 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext } 8, 37 -- X-VPM-CERT-FLAG: 0 } 8, 37 -- X-VPM-IS-HYBRID: 0 } 8, 37 -- Content-Transfer-Encoding: 8bit } 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id u08EQuO4003928 ==============================End } of - Headers==============================
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==============================Original Headers============================== 13, 46 -- From benada-at-biomed.cas.cz Fri Jan-* 8 11:05:37 2016 13, 46 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 13, 46 -- -*-*-* by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08H5asd016283 13, 46 -- -*-*-* for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 11:05:36 -0600 13, 46 -- X-ASG-Debug-ID: 1452272724-05011e54253651e0001-4CH8be 13, 46 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id gMTvxbAFFmBOVAmD (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 08 Jan 2016 18:05:25 +0100 (CET) 13, 46 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 13, 46 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 13, 46 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 13, 46 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 13, 46 -- -*-*-* (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 46 -- -*-*-* (No client certificate requested) 13, 46 -- -*-*-* by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 2E2172C8070; 13, 46 -- -*-*-* Fri,-* 8 Jan 2016 18:05:20 +0100 (CET) 13, 46 -- Date: Fri, 8 Jan 2016 18:05:19 +0100 13, 46 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 13, 46 -- To: tbargar-at-unmc.edu, {microscopy-at-microscopy.com} 13, 46 -- Subject: Re: [Microscopy] Stabilizing pili on bacteria for SEM 13, 46 -- Message-ID: {20160108180519.01b05d54-at-u117ob02} 13, 46 -- X-ASG-Orig-Subj: Re: [Microscopy] Stabilizing pili on bacteria for SEM 13, 46 -- In-Reply-To: {201601081431.u08EVEY2006755-at-ns.microscopy.com} 13, 46 -- References: {201601081431.u08EVEY2006755-at-ns.microscopy.com} 13, 46 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 13, 46 ---* =?UTF-8?B?xIxS?= 13, 46 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 13, 46 -- MIME-Version: 1.0 13, 46 -- Content-Type: text/plain; charset=UTF-8 13, 46 -- X-IoP-CAS-MailScanner-ID: 2E2172C8070.17926 13, 46 -- X-IoP-CAS-MailScanner: Processed 13, 46 -- X-Spam-Status: No 13, 46 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 13, 46 -- X-Barracuda-Start-Time: 1452272725 13, 46 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 13, 46 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 13, 46 -- X-Barracuda-Scan-Msg-Size: 6554 13, 46 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 13, 46 -- X-Barracuda-BRTS-Status: 1 13, 46 -- X-Barracuda-Spam-Score: 0.50 13, 46 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M 13, 46 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25954 13, 46 -- -*-*-* Rule breakdown below 13, 46 -- -*-*-*-*-*pts rule name-* -* -* -* -* -* -* description 13, 46 -- -*-*-* ---- ---------------------- -------------------------------------------------- 13, 46 -- -*-*-* 0.50 BSF_RULE7568M-* -* -* -* -* Custom Rule 7568M 13, 46 -- Content-Transfer-Encoding: 8bit 13, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08H5asd016283 ==============================End of - Headers==============================
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Hahahaa, you guys are so funny. I got 17 replies to my query about turning an iPhone into a microscope, and no two were the same. And most of them were along the lines of "I saw this one on the Internet...".
If anyone is particularly interested, I can send a list of links or forward some of the responses. Here is the one that the person who requested feedback is looking at (and is not one of the ones any of you mentioned):
} My apologies for cross-posting... } } Someone has asked me about microscope attachments for iPhone. I've seen } lots of ads, but I haven't looked closely at any of the products. Do any of } you have opinions about products that turn your iPhone or Android into } decent microscopes? I'm not sure if she wants to go with a stand and } slides, or just take nicely magnified images on the go. I think the } former... } } Aloha, } Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Fri Jan 15 18:20:47 2016 7, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0G0KkRe002113 7, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jan 2016 18:20:47 -0600 7, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0G0KTRt000895 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 21 -- Fri, 15 Jan 2016 14:20:29 -1000 (HST) 7, 21 -- Received: from localhost (tina-at-localhost) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0G0KSnV000891; 7, 21 -- Fri, 15 Jan 2016 14:20:28 -1000 (HST) 7, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Fri, 15 Jan 2016 14:20:28 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-X-Sender: tina-at-b1000 7, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 7, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU 7, 21 -- Subject: iPhone microscope 7, 21 -- Message-ID: {Pine.GSO.4.64.1601151415220.26754-at-b1000} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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Email: Chrissi.Luede-at-gmail.com Name: Christopher L,demann
Organization: RWTH Aachen, GERMANY
Title-Subject: [Filtered] ETEC Autoscan SEM - looking for service manual
Message: Hello everyone,
I rescently acquired a vintage ETEC Autoscan SEM (rebadged by Siemens) and intend to refurbish it as a hobby project.
The instrument came with a basic user manual, but in order to put the electronics back into operation, I am looking for a more detailed service manual (circuit diagrams, exploded drawings, component names, etc.). I would be very grateful, if anyone could share a digitised version of their documents.
And finally, two technical questions: 1. Is there a list of head sizes for the various hex sockets on the electron optical column? Are they metric or imperial? I found a couple of small screws which seem to require something between 2.5...3mm or 1/8...3/32", nothing I would deem as standard...
2. Would you happen to have any documentation on the diffusion pump? I assume it is a Varian type M2. Is is worthwhile to replace it with a turbo pump? I already have all the necessary equipment and would only need to buy an ASA/CF adapter flange plus a vibration damper.
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Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2016
Message: Now accepting registrations for the 46th annual Lehigh Microscopy School which will be held on the campus of Lehigh University, Bethlehem, PA, June 5-10, 2016. All courses, lecturers, and instrument suppliers will be together for what promises to be a phenomenal week! Course offerings include: SEM and X-ray Microanalysis -i Introduction to SEM and EDS for the New Operator -i Focused Ion Beam Instrumentation and Applications -i Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data -i Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques -i Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 15 for an Early Bird Discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.lehigh.edu/microscopy for registration form, prices, and details about courses, lecturers, and instrument suppliers.
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Email: paul.carpenter.epma-at-gmail.com Name: Paul Carpenter
Organization: MAS
Title-Subject: [Filtered] MAS Electron-Probe Microanalysis 2016 workshop
Message: Microanalysis Society Topical Conference Electron-Probe Microanalysis 2016
May 16-19, 2016 at the University of Wisconsin-Madison, WI
Registration is now open See website for full details Abstract deadline February 15, 2016 MAS Early Career Scholar financial travel support
Four-day intensive MAS topical conference
User meetings with EPMA 2016 sponsors on Monday, May 16, 2016 Three-day intensive topical conference, Tues-Thurs May 17-19, 2016 - EPMA, SEM, WDS, EDS techniques
Tutorial on EPMA and SEM quantitative analysis
Topics with invited and contributed presentations
General electron-probe microanalysis: analysis, instrumentation, methods
Trace element EPMA: background methods, high-resolution EPMA
Advanced WDS and EDS techniques, Monte Carlo simulation, accuracy studies
Quantitative compositional mapping by EPMA
Cathodoluminescence
Presentations and product information from the microanalysis vendor community
Inexpensive housing options
Group meals and discussion
Problems identified by the microanalysis community -- solutions provided by the vendor community
Contact Paul Carpenter for more information: paul.carpenter.epma-at-gmail.com
Paul Carpenter Earth and Planetary Sciences CB1169 Washington University 1 Brookings Drive Saint Louis, MO 63130 314-602-9697
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Email: mk-at-seclab.tuwien.ac.at Name: Markus Kammerstetter
Organization: Secure Systems Lab Vienna, Vienna University of Technology
Title-Subject: [Filtered] Looking for EDS for RJ Lee PSEM
Message: Hello,
in our lab we have an older model RJ Lee/Aspex PSEM Scanning Electron Microscope in use to image and analyze microchips. We deprocess those chips layer by layer but currently do not have the exact information of the material compositions used in those layers. Since the deprocessing and material removal techniques (i.e. wet etching) heavily depend on the materials, we have been looking for an EDS system for quite a while but unfortunately do not really have a budget for it.
It would thus be great if anyone on this list could spare a surplus EDS that is no longer needed (detector, control HW and possibly also the SW tool). Ideally it would include light elements and be compatible with the Aspex/RJ Lee PSEM so that we could use the PSEM's software. However, virtually any EDS would also be nice to have if we can make it fit. Also just heavy elements are fine as this would still allow us to characterize Si, Cu, Al, W, Au and other material compositions typically found in integrated circuits.
We have access to LN2 for cooling, we can typically do mechanical and electronics repairs on our own and we could also mill a custom adapter to make it fit onto our PSEM port.
Thank you, Markus
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Email: lnbrewer1-at-eng.ua.edu Name: Luke Brewer
Organization: University of Alabama
Title-Subject: [Filtered] Inviting you to attend and participate in EBSD 2016
Message: Dear Colleagues,
I invite you to attend and participate in Electron Backscatter Diffraction 2016! EBSD 2016 will be held at The University of Alabama from May 24-26, 2016.
EBSD 2016 will have a one-day tutorial session with both lectures and hands-on laboratory demonstrations designed to introduce EBSD to students and professionals new to the technique. There will also be more advanced tutorials for experienced users on topics such as precession electron diffraction, high angular resolution EBSD, and transmission kikuchi diffraction.
Days 2-3 will have platform presentations and posters devoted to the latest in EBSD-technique development and applications to materials science, geology and earth sciences, and materials engineering.
Support will be available to encourage students to attend this meeting.
Registration is now open, and we are accepting abstracts on-line until March 1, 2016.
Full details on the meeting can be found at: http://www.microbeamanalysis.org/topical-conferences/ebsd-2016/welcome
Please let me know if you have any questions, and we do hope to see you at EBSD 2016!
Luke Brewer
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I am having problems finding pre-cleaned microscope slides that are actually clean. I've looked at recently purchased slides form 2 vendors but neither product is "clean" with easily visible particulates and splotchy thin films randomly covering slide surfaces. Is anyone else out there noticing that previously good sources are now "dirty". I am using Polarized and bright field illumination. I would appreciate a product recommendation if you currently have a high quality source.
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Organization: OSU Center for Veterinary Health Sciences
Title-Subject: [Filtered] Seeking a repairperson for a Sorvall MT7000
Message: If anyone has the name and contact information for a person who could provide service for a Sorvall MT-7000, please share this information with me.
Thanks very much.
Kathy Kocan
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Email: wzhe-at-laurentian.ca Name: William
Organization: Laurentian University
Title-Subject: [Filtered] EDS detector window problem
Message: Hello,
We currently had problem with EDS SDD detector , it couldn-it be cooling down and a -eFault-i message for detector condition came out at the end. One recommendation is that is sign of detector window blown or pin hole on it. Is somebody ever went through this type of issue and have ideas about
1. how to identify if it is a window problem? 2. is it a simple job to replace a defective window if you have parts, like people always do for WDS detector on their own ? If not, 3. any service company offers repairing in north America you may recommend ?
Thanks so much in advance,
William
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Application Deadline Monday, January 25. Please spread the word.
Dear Colleagues,
We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.
Laboratory exercises, demonstrations, and discussions include: * geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; * phase contrast, polarization and interference microscopy; * fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; * principles and application of digital imaging and quantitative digital image deconvolution; * digital image processing and object identification and tracking; * live cell and ratiometric imaging for FRET and ion concentration imaging; * confocal microscopy and specialized methods such as TIRF and FLIM; and * new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.
The course web site is at http://www.mbl.edu/aqlm .
Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.
Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.
Applications due January 25 , 2016.
Directors: Jagesh Shah (Harvard Medical School), Justin Taraska (National Institutes of Health) Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)
Cheers, Jagesh, Justin and Wendy
Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility 9 Cambridge Center, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 13, 40 -- From wsalmon-at-wi.mit.edu Sat Jan 23 12:33:24 2016 13, 40 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0NIXIGM016281 13, 40 -- for {microscopy-at-microscopy.com} ; Sat, 23 Jan 2016 12:33:19 -0600 13, 40 -- X-IronPort-AV: E=Sophos;i="5.22,333,1449550800"; 13, 40 -- d="scan'208";a="3640678" 13, 40 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 13, 40 -- by ptolemy.wi.mit.edu with ESMTP; 22 Jan 2016 16:11:38 -0500 13, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id B7D2631E138 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:38 -0500 (EST) 13, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 13, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 13, 40 -- with ESMTP id TZ2FtvMjteGg for {microscopy-at-microscopy.com} ; 13, 40 -- Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 931EE31E13E 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 13, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 13, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 13, 40 -- with ESMTP id MJ_iGgBykbZT for {microscopy-at-microscopy.com} ; 13, 40 -- Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 77FD131E138 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- Date: Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 13, 40 -- To: microscopy {microscopy-at-microscopy.com} 13, 40 -- Message-ID: {344470042.11777.1453497096429.JavaMail.zimbra-at-wi.mit.edu} 13, 40 -- In-Reply-To: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu} 13, 40 -- References: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu} 13, 40 -- Subject: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 13, 40 -- MIME-Version: 1.0 13, 40 -- Content-Type: text/plain; charset=utf-8 13, 40 -- Content-Transfer-Encoding: 7bit 13, 40 -- X-Originating-IP: [134.174.140.59] 13, 40 -- X-Mailer: Zimbra 8.6.0_GA_1191 (ZimbraWebClient - GC47 (Mac)/8.6.0_GA_1191) 13, 40 -- Thread-Topic: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 13, 40 -- Thread-Index: 3I4RFXAc6NuHKgHiTFr8kP+ljEXmnAHyDNBp ==============================End of - Headers==============================
I would like to draw your attention to the call for nominations for the Gjonnes Medal at http://www.iucr.org/resources/commissions/electron-crystallography/gjonnes-medal
The Gj/PInnes Medal in Electron Crystallography is awarded every three years and recognizes an outstanding contribution to the field of electron crystallography. Nomination for the Gj/PInnes Medal is open to scientists and engineers in all areas of electron crystallography defined in the broadest context as the branch of science that uses electron scattering and imaging to study the structure of matter.
Please forward as appropriate.
-- Professor Laurence Marks Department of Materials Science and Engineering Northwestern University www.numis.northwestern.edu Corrosion in 4D: MURI4D.numis.northwestern.edu Co-Editor, Acta Cryst A "Research is to see what everybody else has seen, and to think what nobody else has thought" Albert Szent-Gyorgi
==============================Original Headers============================== 6, 41 -- From marksmsa-at-gmail.com Mon Jan 25 07:56:48 2016 6, 41 -- Received: from mail-lb0-f169.google.com (mail-lb0-f169.google.com [209.85.217.169]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0PDul4Z011783 6, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 07:56:48 -0600 6, 41 -- Received: by mail-lb0-f169.google.com with SMTP id oh2so74306465lbb.3 6, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 05:56:36 -0800 (PST) 6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=gmail.com; s=20120113; 6, 41 -- h=mime-version:date:message-id:subject:from:to:content-type 6, 41 -- :content-transfer-encoding; 6, 41 -- bh=eJTRCxW18amnX96ZxQah12BYXFKTQa7Ha1jEYoMZ138=; 6, 41 -- b=gyVIcug9pncp9WWAZoo5ERd9HCF60vuBClLojdqissl/LR3abuvNmGRVjv8Wcaxi7A 6, 41 -- 76pH4mAU/g90jRjjvrzNsjzY/leEx2i3J0F+X6eVtOB8m8KB+C6dYiPAM6tiXsZ8QnjC 6, 41 -- r1osfEtmJQCnQwvA9YcUTaLO5LObo+YfY1Sl5LcVz4PNTNtgZuyk0WbIHeJbfMjFkswH 6, 41 -- LxzO3k1mRiCTJS/iUpo4tEjMuiVgg8FtQr9IK0UhN02DMi3mMZlE5dCQ3PoKOu20hRu+ 6, 41 -- 6uzh3R6QyOFSQ/T7C9b0dtm13kmegdsqGkuhYJLmM2xqcFO+avrEdP1LgbxrkiRu7boI 6, 41 -- Jfuw== 6, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=1e100.net; s=20130820; 6, 41 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 6, 41 -- :content-type:content-transfer-encoding; 6, 41 -- bh=eJTRCxW18amnX96ZxQah12BYXFKTQa7Ha1jEYoMZ138=; 6, 41 -- b=Ny+1nrnvTJkt0t8NPQ2a7jTY8NAD1+5S9RbTT5RsNaGw6DPJIvbr2xo/58TVCiUlqW 6, 41 -- waI+VhDdyTu9jQRSZKm/ialtDWCddEW3TCg04OnkR0+mMeCHc3pXUvPlJRl0rYYiyllU 6, 41 -- IKQa/4FMJeJWJG0MWa3RLweZ/e3cyfgsaZ8Um33HTJROA3050DWq/2Al6itos3yc/9+U 6, 41 -- 6Kvgk6KOScryunGvpvBKI8DZqcGD/ZcCwUFKcNDPWhisEWME6+Rmr7cMsLqgzO9NkH/f 6, 41 -- 6kelqa9XxpaRRW+TtfOSS53cg0r5QTQ/beW+kvwm4ajTIhimGn/F2unmsBpDoQvFFa8z 6, 41 -- QhiA== 6, 41 -- X-Gm-Message-State: AG10YORXrJCnmVDOH8bqtrsumFzWaXn8w4NLkuUSDZUgHLcuxmnHmtHhmfMVDtZnivIvUy9DL8JiVTKL6TOHxA== 6, 41 -- MIME-Version: 1.0 6, 41 -- X-Received: by 10.112.73.41 with SMTP id i9mr1453083lbv.128.1453475883194; 6, 41 -- Fri, 22 Jan 2016 07:18:03 -0800 (PST) 6, 41 -- Received: by 10.112.126.228 with HTTP; Fri, 22 Jan 2016 07:18:03 -0800 (PST) 6, 41 -- Date: Fri, 22 Jan 2016 09:18:03 -0600 6, 41 -- Message-ID: {CAHuu12pZ6bd3S_XxFKtc6sBCFB3bT439A0zhhED7K+PV5cX7Fg-at-mail.gmail.com} 6, 41 -- Subject: 6, 41 -- From: L Marks {marksmsa-at-gmail.com} 6, 41 -- To: microscopy {Microscopy-at-microscopy.com} 6, 41 -- Content-Type: text/plain; charset=UTF-8 6, 41 -- Content-Transfer-Encoding: 8bit 6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0PDul4Z011783 ==============================End of - Headers==============================
I wrote a software called stoichometry fitter for doing quick analyses of spectra acquired by EDS (or XRF). Originally, this was just for my own use but it has evolved to the point where I think it may be useful for some of you. I have now made it open source and put it on GitHub for anyone who wants to use it or contribute to it.
As input it takes At%, Wt% or OxWt% values.
It has the ability to fit several phases, for example, if you have a pyroxene which you think is contaminated with a bit of FeS, you can fit against a pyroxene triangle + FeS, and it will give you the correct EnFsWo values after removing the appropriate amount of Fe for the FeS. It also has a freeform analysis capability for a few minerals I use, and I'm hoping others will contribute their own analyses as well so we can all use them. For example, if you tell it the spectrum is a pyroxene, then it will compute T, M1 and M2 sites for all the elements using the Morimoto 1988 reference. If you are handy with python and numpy then you can create your own analyses with a minimum of four lines of code.
Finally, it has a TEM k-factor + thickness correction capability if you input each element as counts instead of the usual At%/Wt%/OxWt%. This is very useful if you have a TEM, but it would also apply to some thin-film experiments in an SEM/EPMA.
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School
Message: April 12-15, 2016 Gatan R&D Headquarters Pleasanton, CA, USA
This comprehensive hands-on training course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of EELS and EFTEM hardware and software systems, and advanced applications. Some prior experience with EELS, EFTEM, and energy filter systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples. Most of the curriculum is devoted to live microscope and computer lab sessions.
Topics:
-iFundamentals of EELS and energy-filtered imaging in TEM -iPrinciples of operation of EFTEM and EELS systems -iOptimization of EFTEM and EELS data acquisition -iQuantification of elemental composition -iOther information provided by EFTEM/EELS and how best to extract it -iUse of EELS signals to form maps of elemental and chemical composition -iEFTEM and STEM EELS spectrum imaging techniques -iIdentification of material phases via EELS fine structure mapping -iApplications to biological and physical science specimens
Complete information and online registration is available at: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2016
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Email: david-at-electronmicro.com Name: David Scharf
Organization: SCHARF MICROSCOPY
Title-Subject: [Filtered] LEO440 CPU CMOS battery
Message: Can anyone help? I am getting a message that my CMOS battery has failed upon booting up my LEO440 SEM. I have looked everywhere I can think of and cannot find that battery to replace it. It is NOT on the computer board for sure! Must be external somewhere. Anyone service this instrument before and know where its located? No mention of its location in my service manuals either. Thanks in advance for any help.
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Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections
Message: Can anyone recommend a technique for collecting rubber sections during cryo-ultramicrotomy that enables the sections to lie flat (i.e. as wrinkle free as possible) on a TEM support grid? I currently collect my sections off the back of my cryoknife using a sucrose droplet, but as the sections warm coming out of the cryochamber on the droplet they contract and wrinkle before being deposited on a TEM grid (with or without carbon coating). Chamber temperature is -120 C. Any ideas are appreciated. Thank you.
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Symposium in Advanced Electron Microscopy in Nanostructure Research and Inauguration of the new Titan Themis 300 S/TEM.
We are glad to announce and invite you to a full-day symposium in iAdvanced Electron Microscopy in Nanostructure Researchi and the inauguration of the new Titan Themis 300 S/TEM and the Quanta 200 duo beam instrument at the Central Facility for Advanced Microscopy and Microanalysis (CFAMM).
The inauguration will take place on March 10th, 2016 starting at 9 a.m. in 355 HUB building at the University of California at Riverside. Plenary seminar talks will be presented by leading researchers demonstrating the application of S/TEM and duo-beam techniques in nanostructure research. The plenary session will be followed by demo sessions on the instruments in the afternoon.
Central Facility for Advanced Microscopy and Microanalysis, Research & Economic Development Office Materials Science and Engineering - BCOE University of California Riverside, CA 92521
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Email: wtkiii-at-hotmail.com Name: WILLIAM KING
Title-Subject: [Filtered] TEM & SEM of intestinal cells
Message: I would like to know why the antigen behind Ulcerative Colitis has not been identified. If the affected tissue were available, how many micrographs would it take to provide some visual cues?
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscopy Society of Northeastern Ohio, MSNO
Message: Our MSNO Winter Meeting 2016 will go to a new and exciting place: Lorain County Community College. The meeting will be on March 2nd, 2016 3-7:15 pm. Please see the full program from attached below.
The event will combine professional networking, lectures on by leading expert, guided tours & demos at the impressive LCCC Desich Center, student posters, exhibitions by leading vendors in the field, a nice dinner, a fun raffle and much more. Here are some of the highlights: - Two main talks on Applications for MEMS Sensors (Matthew Apanius, Managing Director, SMART Microsystems, Ltd) and "Basic Digital Imaging and Image Form" (by Dr. W. Gray Jerome, Vanderbilt University Medical Center, a National MSA tour speaker).
- Tour at the Desich Center
The registration and more detail of this meeting can be found at http://www.msneo.org/2016-winter-meeting.html
Looking forward to seeing you at LCCC! MSNO Board
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One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software).
I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me.
Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.)
I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples?
This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer.
Thanks in advance Chad Parish
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 10, 53 -- From parishcm-at-ornl.gov Thu Jan 28 06:52:26 2016 10, 53 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SCqQkd021923 10, 53 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 06:52:26 -0600 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 10, 53 -- t=1453985534; x=1485521534; 10, 53 -- h=from:to:subject:date:message-id: 10, 53 -- content-transfer-encoding:mime-version; 10, 53 -- bh=0K5YMDyPwYQooheRgxj/FTgKf0AHz08TVaqOkaSqnjk=; 10, 53 -- b=JQHfmgS1J7qCem6b4i9bUlY4nbNpNCehgsPzwhe5Ww+0fgUAvLHIzX2F 10, 53 -- HXJaUjP8fWBzrbD13bzEFwAE9DsHX320NirvzY78txNEbfxvWswwFMLz9 10, 53 -- j87523vFotYQsRFDnKxi2afbOHAkx5g4UkuIpYOXjuOKQG+SzzAdhjq+b 10, 53 -- 3pWaGGmnm9pGLVUkl893mvPox9XMfryEjuwEqrxo2b9KqKsXreT1HzNco 10, 53 -- yHl3l0oB3DLTLWU5JfeR4RcV4LyJs8g8YmQ2JWeSLl4ugPSrnbAkJwzgz 10, 53 -- AI/6xFhSZnwnR3fLyJx/Udz8vTLqC6DAa9wK8ncJSmFRD+kTEuMO90/n7 10, 53 -- w==; 10, 53 -- X-SG: RELAYLIST 10, 53 -- X-IronPort-AV: E=Sophos;i="5.22,358,1449550800"; 10, 53 -- d="scan'208";a="96755483" 10, 53 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 10, 53 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 28 Jan 2016 07:52:13 -0500 10, 53 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) 10, 53 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 10, 53 -- (No client certificate requested) 10, 53 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3prhXF6W9Zz7tK9 10, 53 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jan 2016 07:52:13 -0500 (EST) 10, 53 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov 10, 53 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan 10, 53 -- 2016 07:52:13 -0500 10, 53 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS34.ornl.gov 10, 53 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan 10, 53 -- 2016 07:52:13 -0500 10, 53 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 10, 53 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 10, 53 -- 15.00.1104.000; Thu, 28 Jan 2016 07:52:13 -0500 10, 53 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 10, 53 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates 10, 53 -- Thread-Topic: FEI SuperX / Bruker: Strange performance at very low count rates 10, 53 -- Thread-Index: AdFZyrKEswZ9BvjyTQmAzHMpryukEA== 10, 53 -- Date: Thu, 28 Jan 2016 12:52:12 +0000 10, 53 -- Message-ID: {16c8fb3650e14a9981accfc0506e11b5-at-EXCHCS32.ornl.gov} 10, 53 -- Accept-Language: en-US 10, 53 -- Content-Language: en-US 10, 53 -- X-MS-Has-Attach: 10, 53 -- X-MS-TNEF-Correlator: 10, 53 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 53 -- x-originating-ip: [128.219.12.132] 10, 53 -- Content-Type: text/plain; charset="us-ascii" 10, 53 -- MIME-Version: 1.0 10, 53 -- Content-Transfer-Encoding: 8bit 10, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SCqQkd021923 ==============================End of - Headers==============================
The Hooke College of Applied Sciences, located in Westmont, IL, is offering its scanning electron microscopy short course March 14-18, 2016.*
In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.* For further SEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
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we have a JEOL X-ray detector with a Thermo Pulse-processor and software and we can go way down in beam current to still collect X-rays. On a recent trip our engineer showed me a software switch to remove the "noise" peak counts from the spectrum and the reported count rate.
But if you are literally not seeing any peaks with 200pA then there's a fundamental problem - call Bruker at once.
Good luck.
Rob Keyse
On 1/28/2016 8:12 AM, parishcm-at-ornl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software). } } I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me. } } Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.) } } I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples? } } This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer. } } Thanks in advance } Chad Parish } } --------------------- } Chad M. Parish, Ph.D. } Research and Development Staff Member } Radiation Effects and Microstructural Analysis Group } Materials Science and Technology Division } Oak Ridge National Laboratory } Phone: 1 865 574 0092 } Email: parishcm-at-ornl.gov } } } } ==============================Original Headers============================== } 10, 53 -- From parishcm-at-ornl.gov Thu Jan 28 06:52:26 2016 } 10, 53 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) } 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SCqQkd021923 } 10, 53 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 06:52:26 -0600 } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; } 10, 53 -- t=1453985534; x=1485521534; } 10, 53 -- h=from:to:subject:date:message-id: } 10, 53 -- content-transfer-encoding:mime-version; } 10, 53 -- bh=0K5YMDyPwYQooheRgxj/FTgKf0AHz08TVaqOkaSqnjk=; } 10, 53 -- b=JQHfmgS1J7qCem6b4i9bUlY4nbNpNCehgsPzwhe5Ww+0fgUAvLHIzX2F } 10, 53 -- HXJaUjP8fWBzrbD13bzEFwAE9DsHX320NirvzY78txNEbfxvWswwFMLz9 } 10, 53 -- j87523vFotYQsRFDnKxi2afbOHAkx5g4UkuIpYOXjuOKQG+SzzAdhjq+b } 10, 53 -- 3pWaGGmnm9pGLVUkl893mvPox9XMfryEjuwEqrxo2b9KqKsXreT1HzNco } 10, 53 -- yHl3l0oB3DLTLWU5JfeR4RcV4LyJs8g8YmQ2JWeSLl4ugPSrnbAkJwzgz } 10, 53 -- AI/6xFhSZnwnR3fLyJx/Udz8vTLqC6DAa9wK8ncJSmFRD+kTEuMO90/n7 } 10, 53 -- w==; } 10, 53 -- X-SG: RELAYLIST } 10, 53 -- X-IronPort-AV: E=Sophos;i="5.22,358,1449550800"; } 10, 53 -- d="scan'208";a="96755483" } 10, 53 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) } 10, 53 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 28 Jan 2016 07:52:13 -0500 } 10, 53 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) } 10, 53 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) } 10, 53 -- (No client certificate requested) } 10, 53 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3prhXF6W9Zz7tK9 } 10, 53 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jan 2016 07:52:13 -0500 (EST) } 10, 53 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov } 10, 53 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan } 10, 53 -- 2016 07:52:13 -0500 } 10, 53 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS34.ornl.gov } 10, 53 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan } 10, 53 -- 2016 07:52:13 -0500 } 10, 53 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by } 10, 53 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id } 10, 53 -- 15.00.1104.000; Thu, 28 Jan 2016 07:52:13 -0500 } 10, 53 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} } 10, 53 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} } 10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates } 10, 53 -- Thread-Topic: FEI SuperX / Bruker: Strange performance at very low count rates } 10, 53 -- Thread-Index: AdFZyrKEswZ9BvjyTQmAzHMpryukEA== } 10, 53 -- Date: Thu, 28 Jan 2016 12:52:12 +0000 } 10, 53 -- Message-ID: {16c8fb3650e14a9981accfc0506e11b5-at-EXCHCS32.ornl.gov} } 10, 53 -- Accept-Language: en-US } 10, 53 -- Content-Language: en-US } 10, 53 -- X-MS-Has-Attach: } 10, 53 -- X-MS-TNEF-Correlator: } 10, 53 -- x-ms-exchange-transport-fromentityheader: Hosted } 10, 53 -- x-originating-ip: [128.219.12.132] } 10, 53 -- Content-Type: text/plain; charset="us-ascii" } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- Content-Transfer-Encoding: 8bit } 10, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SCqQkd021923 } ==============================End of - Headers==============================
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Office (610)758-3465 Fax (610)758-4244
==============================Original Headers============================== 10, 33 -- From rok210-at-lehigh.edu Thu Jan 28 08:22:19 2016 10, 33 -- Received: from rain4.cc.lehigh.edu (rain4.cc.lehigh.edu [128.180.3.218]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SEMJmr000752 10, 33 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 08:22:19 -0600 10, 33 -- Received: from [127.0.0.1] (rok210pc.mat.lehigh.edu [128.180.54.183]) 10, 33 -- (Authenticated sender: rok210) 10, 33 -- by rain4.cc.lehigh.edu (Postfix) with ESMTPSA id 0CEAA503AF87; 10, 33 -- Thu, 28 Jan 2016 09:22:07 -0500 (EST) 10, 33 -- Subject: Re: [Microscopy] FEI SuperX / Bruker: Strange performance at very low 10, 33 -- count rates 10, 33 -- To: parishcm-at-ornl.gov, microscopy-at-microscopy.com 10, 33 -- References: {201601281312.u0SDCisw001826-at-ns.microscopy.com} 10, 33 -- From: Robert Keyse {rok210-at-lehigh.edu} 10, 33 -- Message-ID: {56AA240E.7040000-at-lehigh.edu} 10, 33 -- Date: Thu, 28 Jan 2016 09:22:06 -0500 10, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 10, 33 -- Thunderbird/38.5.1 10, 33 -- MIME-Version: 1.0 10, 33 -- In-Reply-To: {201601281312.u0SDCisw001826-at-ns.microscopy.com} 10, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 33 -- Content-Transfer-Encoding: 7bit 10, 33 -- X-Antivirus: avast! (VPS 160128-0, 01/28/2016), Outbound message 10, 33 -- X-Antivirus-Status: Clean 10, 33 -- X-Virus-Scanned: clamav-milter 0.98.7 at rain4.CC.lehigh.EDU 10, 33 -- X-Virus-Status: Clean 10, 33 -- X-Spam-Status: No, score=-4.0 required=5.0 tests=ALL_TRUSTED,LU_FORGED_SENDER, 10, 33 -- RP_MATCHES_RCVD autolearn=disabled version=3.4.1 10, 33 -- X-Spam-Report: 10, 33 -- * -5.0 ALL_TRUSTED Passed through trusted hosts only via SMTP 10, 33 -- * -0.0 RP_MATCHES_RCVD Envelope sender domain matches handover relay domain 10, 33 -- * 1.0 LU_FORGED_SENDER No description available. 10, 33 -- X-Spam-Checker-Version: SpamAssassin 3.4.1 (2015-04-28) on 10, 33 -- freddie.cc.lehigh.edu ==============================End of - Headers==============================
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Email: ming_j_zhang-at-amat.com Name: Ming Zhang
Organization: Applied Materials Company
Title-Subject: [Filtered] FIB Technician Opening
Message: The successful candidate will focus on TEM sample preparations by hands-on operations of ex-situ and in-situ lift-out systems.
Term: Must be available for a minimum of 6 months. Term could be extended to 18 months. Highly possible to turn into a regular full time position in 6-12 months.
Work Schedule: Weekend and night shifts.
Education: Associate degree or equivalent combination of education and experience in Engineering, Materials Science, Chemistry, or Physics, etc.
Experience: Hands-on experience on FIBs or dual beams for TEM sample preparations. Manual TEM sample prep a plus.
Other Skills: Must be a team player and interact in a positive fashion with peers and customers. Must be able to work in a fast paced, multi-tasking environment where priorities may change often.
Location: Portland, Oregon.
US citizen or green card holder preferred.
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Long ago, when histology was in vogue, I had a problem during the summer producing paraffin sections of good repute when the windows were full open, the temp was above 80, humidity was high and Summer was the bane of my progress.
If you have a fancy microtome that has a means of changing the angle of the knife, then you can refine the method that I used with a self-constructed series of thin, plywood templates that would permit me to change the setting of my blade as the weather moved thru its cycle. My goal, when the change was in the wind, was to produce a section whose height was greater than all the others I could produce with angles above and below the one that worked the best.
Air conditioning had just arrived, but not in our lab, so, I was stuck with my templates. Since I was working in a building with large windows, no cooling but the breeze on the mountain, patience and changing angles and, oh yes, changing the hardness of the paraffin as the weather went thru its yearly cycle. If one moved South to Florida to cut sections, one would have to fabricate a Florida paraffin mixture as an embedment - much too hard for even Spring or Fall in Bethlehem, PA.
So, we come to the advice. The first problem is compression. The second is the sharpness of the blade. The third is the vibrations that emanate from the microtome that can, in the first approximation, be reduced with a few drops of lubricant. Then, there is the vibration of the floor, the building, and the planet, and the solidity of the blade in its holder, the geometry of the blade and the angles of the cutting wedge on the edge of the blade. Sorry, I digress.
The optimal angle lies within a small range of not-so-optimal angles that are dependent on the hardness of the paraffin or plastic, the temp of the environment, the geometry of the cutting edge, the humidity, and everything else.
As I recall, my instructor, who had a PhD from Harvard under Alfred Romer, instructed me on the microtome and its personalities by advising me to, "fiddle with the block, the knife, the angle of the knife, the rate of the cutting cycle, and everything else until you get good sections." He ended with, "That's how I always did it."
Now that I've said and reviewed all of this I conclude that I have not been very helpful. That is primarily due to the fact that I haven't cut a section from a paraffin block since 1992, when I taught my younger daughter to cut sections for my research. As I recall, I used the word "fiddle" more than once during the tutorial, but again that was then and we sharpened our knives ourselves. She, by the way, was the best, and happiest 'cranker' I ever trained, and I convinced a small army to be good at it.
I hope that at the very least you are happy that you are younger, and don't have to do your sectioning in a room with the windows open - even in Winter!
Seicerely,
Fred Monson (he works on electron microscopes, hardly ever has to cut sections. and is contentedly moving toward 77 with good health and a lively sense of humor.
P.S. The adjustment of the knife angle is similar to the problem a baseball player has with his bat. He fiddles with everything to reduce the frequency of his pop ups. Neither are like the violin on which you are guaranteed that you will be world famous if you practice for 10,000 hours - either for the best or the worst noises you can project from the instrument.
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu ________________________________________ X-from: Kelli Goodkowsky via Histonet {histonet-at-lists.utsouthwestern.edu} Sent: Friday, January 29, 2016 3:15 PM To: histonet-at-lists.utsouthwestern.edu
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Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] TEM: agar embedding cells
Message: Some advice please on colorizing agar for embedding cells. In the past, my osmicated pellets in agar blocks were easily trackable, but when the cells are in a thin layer the cells don't give much contrast, especially if not osmicated. I am collecting naked eukaryotic cells in suspension culture, but have problems bring down the smaller cells by centrifugation. Cultures containing cells are fixed in aldehyde or aldehyde/osmium cocktail, collected onto millipore Nucleopore filters, then held in place by adding warm agar over them on the filter.
When cooled, I peel off the filter, leaving me with a thin layer of cells embedded in the agar. I then cut the agar layer into small blocks to enhance fluid exchange during subsequent treatments. My problem is, the cells are light colored to faintly black, and the thin layer of cells does not stand out well, particularly when in the various stages of epon infiltration/polymerization. I tried adding some azure II blue dye I had on hand to the agar so I could see the agar blocks more easily, but by the time I got up to epon/acetone, the color was gone. Any suggestions for a dye that will withstand both the water based and acetone solutions, or an alternative way to mark the little agar blocks so I can more easily monitor them throughout the process?
Thanks in advance
Steve
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Email: vicenzie-at-si.edu Name: Ed Vicenzi
Organization: Smithsonian Institution
Title-Subject: [Filtered] Senior Scientist, Microscopy & Microanalysis - position available
Message: [16-LM-CR] Senior Scientist, Microscopy & Microanalysis. A Sr. Microscopist will characterize widely diverse samples, including organic and inorganic chemicals, household goods, pharmaceuticals, foods, plastics, etc. Analyst must execute experimental studies and deliver high quality data using a broad range of analytical methods including SEM, EDX, FTIR microscopy, Polarized Light microscopy, Fluorescence, Image analysis. Proficiency with sample preparation methods (sectioning, mounting microtome, etc.) required. Familiarity with GMP/GLP and excellent writing skills. PhD in Analytical Chemistry - 3+ years industrial experience, M.S. -n 5+ years industrial experience. Background in Mineralogy a plus
Please direct questions regarding this position to:
======================== Jonathan Chun, Ph.D. Alliance Technologies 9 Deer Park Dr. Suite B Monmouth Jct., NJ 08852 732-355-1234 (ph) jchun-at-alliancetechgroup.com www.alliancetechgroup.com
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Email: 13qw9-at-queensu.ca Name: Jason
Organization: Queen's University
Title-Subject: [Filtered] TEM FEI low background double tilt holder tip repair
Message: Hi there,
We are encountering an issue with our FEI low background double tilt holder. Probably because of some deformation on the holder tip, now the two small pins cannot hold the sample cradle in position. Does anyone have any experiences in fixing similar holders or know any company that I can contact?
Thanks,
One colleague (Fei) has tried to send the same message with no luck. Not sure what's the reason. Apologizes for letting you receive it twice.
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Hi, I have had luck with fast green added at the 100% ethanol step. I make a saturated fast green solution in ethanol and add a drop or two at the 100% ethanol step. It kept the agar blocks rather colored through subsquent infiltrations and polymerization. Sorry I don't have handy what "saturated" means in terms of mg/mL but if you are interested I could probably dig that up somewhere. Good luck, Tobias
On 1/29/16 8:44 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: sbarlow-at-mail.sdsu.edu } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both sbarlow-at-mail.sdsu.edu as well as the Microscopy } Listserver } --------------------------------------------------------------------------- } } Email: sbarlow-at-mail.sdsu.edu } Name: Steve Barlow } } Organization: SDSU EM Facility } } Title-Subject: [Filtered] TEM: agar embedding cells } } Message: Some advice please on colorizing agar for embedding cells. In } the past, my osmicated pellets in agar blocks were easily trackable, but } when the cells are in a thin layer the cells don't give much contrast, } especially if not osmicated. I am collecting naked eukaryotic cells in } suspension culture, but have problems bring down the smaller cells by } centrifugation. Cultures containing cells are fixed in aldehyde or } aldehyde/osmium cocktail, collected onto millipore Nucleopore filters, } then held in place by adding warm agar over them on the filter. } } When cooled, I peel off the filter, leaving me with a thin layer of } cells embedded in the agar. I then cut the agar layer into small blocks } to enhance fluid exchange during subsequent treatments. My problem is, } the cells are light colored to faintly black, and the thin layer of } cells does not stand out well, particularly when in the various stages } of epon infiltration/polymerization. I tried adding some azure II blue } dye I had on hand to the agar so I could see the agar blocks more } easily, but by the time I got up to epon/acetone, the color was gone. } Any suggestions for a dye that will withstand both the water based and } acetone solutions, or an alternative way to mark the little agar blocks } so I can more easily monitor them throughout the process? } } Thanks in advance } } Steve } } Login Host: 146.244.234.237 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ University of Mass. / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ Amherst, Massachusetts / /___ / \ \___/ \_____ USA 413-545-1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 4, 23 -- From baskin-at-bio.umass.edu Mon Feb 1 16:40:07 2016 4, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u11Me6ng007888 4, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Feb 2016 16:40:07 -0600 4, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 4, 23 -- (authenticated bits=0) 4, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u11MdrUG010762 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 4, 23 -- Mon, 1 Feb 2016 17:39:53 -0500 4, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 4, 23 -- Subject: Re: [Microscopy] viaWWW:TEM: agar embedding cells 4, 23 -- To: Microscopy-at-microscopy.com, sbarlow-at-mail.sdsu.edu 4, 23 -- References: {201601300144.u0U1irGV011027-at-ns.microscopy.com} 4, 23 -- Message-ID: {56AFDEB9.8020907-at-bio.umass.edu} 4, 23 -- Date: Mon, 1 Feb 2016 17:39:53 -0500 4, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 4, 23 -- Gecko/20100101 Thunderbird/38.5.1 4, 23 -- MIME-Version: 1.0 4, 23 -- In-Reply-To: {201601300144.u0U1irGV011027-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Mon, 01 Feb 2016 17:39:53 -0500 (EST) 4, 23 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
Not directly answering your question but making a remark:
You are working with a cell monolayer, meaning approx. 50-um thin layer. This is so thin that you don't have to care about fluid exchange, I don't think that cutting your agar in small blocks will add anything. I would keep the whole agar block standing at the bottom of a jar, so you always know that the cells are on the upper side.
Regards Stephane
-------------------------------------------- On Mon, 2/1/16, baskin-at-bio.umass.edu {baskin-at-bio.umass.edu} wrote:
Subject: [Microscopy] Re: viaWWW:TEM: agar embedding cells To: nizets2-at-yahoo.com Date: Monday, February 1, 2016, 11:48 PM
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Hi, -* -*-*-*I have had luck with fast green added at the 100% ethanol step. I make a saturated fast green solution in ethanol and add a drop or two at the 100% ethanol step. It kept the agar blocks rather colored through subsquent infiltrations and polymerization. Sorry I don't have handy what "saturated" means in terms of mg/mL but if you are interested I could probably dig that up somewhere. Good luck, -* -* -* -* -* -* -* -* -* -* -* -*-*-*Tobias
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The Hooke College of Applied Sciences, located in Westmont, IL, is offering its transmission electron microscopy short course April 5-7, 2016. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further TEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) www.hookecollege.com
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Email: h.wu2-at-lboro.ac.uk Name: Houzheng Wu
Organization: Loughborough University UK
Title-Subject: [Filtered] Job opening: Research Associate in Nuclear Graphite
Message: Research Associate in Nuclear Graphite http://www.jobs.ac.uk/job/AMX298/research-associate-in-nuclear-graphite/
Loughborough University - Department of Materials Location: Loughborough, UK Salary: #28,982 to #31,656 per annum Hours: Full Time Contract Type: Contract / Temporary Placed on: 2nd February 2016 Closes: 7th February 2016 Job Ref: REQ15383
Fixed term for 33 months Understanding and Improving Graphite for Advanced Nuclear Fission (UNIGRAF) Required to undertake experimental research on structure from atomic- to micro-scale of iso-graphite for advanced nuclear reactors. The project aims to improve the capability of nuclear graphite through a better understanding of irradiation-induced changes in structure and their impact on mechanical/physical behavior. The post is part of an EPSRC funded project "UNIGRAF" in partnership with Oxford and Bristol University, and multi supporters in USA, China and Germany. Based at Loughborough the post holder will have strong collaborations with scientists/engineers from all partners, and focus on structure characterisation before and after irradiation using HRTEM, EELS, FIB and other techniques. You will have opportunity to access structural characterisation facilities in Oak Ridge National Laboratory, Oxford, and EPSRC National Facility, and multi overseas travel is expected.
A good honours degree (2.1 minimum) and a PhD or equivalent in Materials Science, Physics, or another relevant discipline and experience in materials structural characterisation techniques is essential. Research in graphite/graphene materials and knowledge in nuclear materials is desirable. Application closing date: 7 February 2016
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Email: skmeno1-at-nps.edu Name: Sarath K Menon
Organization: NAVAL POSTGRADUATE SCHOOL
Title-Subject: [Filtered] Electron Microscopy Position at Naval Postgraduate School, Monterey, CA
Message: Research Assistant Professor, AD-1701-03 Department of Mechanical and Aerospace Engineering Naval Postgraduate School, Monterey, CA
The Mechanical and Aerospace Engineering Department of the Naval Postgraduate School is inviting applications from electron Microscopy Specialists to fulfill a Research Faculty position to support the research and development of a variety of materials including metals, ceramics, carbon-based materials and composites. The position will be focused on the operation of several advanced characterization techniques, including fully equipped Scanning Electron Microscope and Transmission Electron Microscope. Responsibilities of the position include the maintenance and upkeep of these complex analytical instruments. The applicant should possess operational expertise with SEM, FIB, TEM lamellae preparation, TEM operation including STEM methods, high resolution TEM and analytical techniques such as EDS, EBSD, EELS and EFTEM. The candidate for the position must be able to perform sample analysis with minimal direction and/or oversight from technical principal investigators. The candidate must also be able to collect, assimilate, and interpret data for technical principal investigators and write technical reports to document results. The position description includes also the coordination of all services required to keep instruments operational, the procurement of all consumables for the microscopy lab and to carry out all the routine maintenance tasks. A PhD in Materials Science or a closely related field is required. A few years of hands-on experience with current state-of-the-art instrumentation and techniques in the area of electron microscopy (SEM, TEM, STEM, EFTEM and FIB) is mandatory. The ability to teach a course related to Advanced Materials Characterization techniques will be highly valued. In addition, the candidate is expected to train Masters level students to use instruments and help them with data analysis. The ability to obtain a US security clearance is mandatory.
Applications should be received by 1 April 2015 and include a curriculum vitae, writing sample, summary of available teaching evaluations, and three letters of recommendation. Please address the applications to:
Garth V. Hobson Professor and Chair Department of Mechanical & Aerospace Engineering 700 Dyer Road, Bldg 245 -n Watkins Hall, WA-338 Naval Postgraduate School Monterey, CA 93943 TEL: 831-656-2586/831-656-2888 www.nps.edu/mae gvhobson-at-nps.edu
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Email: AThiessen-at-wisc.edu Name: Anna Thiessen
Organization: Electron Microscopy Program/ Madison Area Technical College
Title-Subject: [Filtered] Calling all Graduates
Message: Hey Listservers we need your help! The Electron Microscopy Program at Madison Area Technical College in Madison, Wisconsin is closing its door after 24 years. The present and past program directors (Michael Kostrna and Glenn Boda) are trying to organize a reunion/-iwake-i for the program at the end of May. Unfortunately, after 24 years a lot of contact information has been changed and not updated! If you or anyone you know graduated or participated in the program please pass the word on. If you would like to find out more information, please contact Jennifer Kostrna at kostrna.emwakeparty-at-gmail.com.
Many Thanks, Anna Thiessen (Recent Graduate)
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Title-Subject: [Filtered] Free Kevex Sigma EDX System for SEM
Message: TSS has one complete Kevex Sigma EDX system, that needs a good home. Detector (window looks good), collimator, Sigma Analyzer, 4855 Digital Beam Control Interface Module, and cable set. Was installed on XL 30 W SEM.
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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This could be a couple of things. First did you use phosphate buffer? If your concentration was a 100 mM or over, the Ga, osmium and PO4 can precipitate in the tissue. Check unstained sections to confirm. Also make sure all the Ga is rinsed out of tissue before you get to osmium. Are the tissue pieces small? You can also microwave fixative to get better penetration. Be careful with using reduced osmium and then staining muscle. Lipid granules are affected with the post-section staining with UA and Pb. Apparently the lipid material gets lost after staining and rinsing, I have seen this phenomena and sometimes it will contaminate parts of the section. If you are sure it isn't your staining protocol, then it must be the fixation.
Good Luck, Michael Delannoy
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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This could be a couple of things. First did you use phosphate buffer? If your concentration was a 100 mM or over, the Ga, osmium and PO4 can precipitate in the tissue. Check unstained sections to confirm. Also make sure all the Ga is rinsed out of tissue before you get to osmium. Are the tissue pieces small? You can also microwave fixative to get better penetration. Be careful with using reduced osmium and then staining muscle. Lipid granules are affected by the post-section staining with UA and Pb. Apparently the lipid material gets lost after staining and rinsing, I have seen this phenomena and sometimes it will contaminate parts of the section. If you are sure it isn't your staining protocol, then it must be the fixation.
Good Luck, Michael Delannoy
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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thank you for your thorough listing of your processing step... what you omit, what you changed, etc., etc... Nevertheless you ought to tell us about the == buffer vehicle in fixation as well as after fixation == osmication data == processing schedule right after osmium into ascending alcohols, the latter most important to know which concentration you start. Could it be that you face "salt & pepper" artifact? It would be interesting to see an image of your speckles...
Best regards, Wolfgang
Wolfgang MUSS, PhD Member of MSA until 2015 Retired by Dec. 1st, 2015 SALZBURG, AUSTRIA wij.muss-at-aon.at
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This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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==============================Original Headers============================== 13, 23 -- From wij.muss-at-aon.at Fri Feb 5 04:40:26 2016 13, 23 -- Received: from bsmtp8.bon.at (bsmtp8.bon.at [213.33.87.20]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15AeL6M022046 13, 23 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 04:40:21 -0600 13, 23 -- Received: from anwender1976b3 (unknown [93.83.25.98]) 13, 23 -- by bsmtp8.bon.at (Postfix) with ESMTPA id 3pxYD82BkDz5tlR; 13, 23 -- Fri, 5 Feb 2016 11:40:08 +0100 (CET) 13, 23 -- From: "Wolfgang Muss" {wij.muss-at-aon.at} 13, 23 -- To: {debrat-at-bcm.edu} 13, 23 -- Cc: {microscopy-at-microscopy.com} 13, 23 -- References: {201602042005.u14K5SeB022620-at-ns.microscopy.com} 13, 23 -- In-Reply-To: {201602042005.u14K5SeB022620-at-ns.microscopy.com} 13, 23 -- Subject: [Microscopy] Re: Speckled TEM Samples 13, 23 -- Date: Fri, 5 Feb 2016 11:40:59 +0100 13, 23 -- Message-ID: {004201d16001$b3cd8740$1b6895c0$-at-muss-at-aon.at} 13, 23 -- MIME-Version: 1.0 13, 23 -- Content-Type: text/plain; 13, 23 -- charset="iso-8859-1" 13, 23 -- X-Mailer: Microsoft Office Outlook 12.0 13, 23 -- Thread-Index: AdFfqKW5Na3c1P5rRhWKa2WMh1An2wAV/XRw 13, 23 -- Content-Language: de 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15AeL6M022046 ==============================End of - Headers==============================
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Hello Debra, Thank you for showing us the image with "speckles". I think that the problem might be in lead staining procedure. Please, look at Arvid B. Maunsbach & Bj/drn A. Afzelius "Biomedical Electron Microscopy (Illustrated Methods and Interpretations)" 1999, Pages 207,Aei228 {doi:10.1016/B978-012480610-8/50011-1} for explanation. We had also similar problems with lead carbonate precipitations in the past. Since our technician started to wear a face shield during sections staining with lead citrate the precipitates (contamination) in them almost vanished.
There is a old paper on "How to remove such precipitates from ultrathin sections": Kuo, J. (1980), A simple method for removing stain precipitates from biological sections for transmission electron microscopy. Journal of Microscopy, 120: 221,Aei224. {doi:10.1111/j.1365-2818.1980.tb04140.x}
We tried this approach several times, but the results were not ideal in our hands.
Best regards
Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 13, 45 -- From benada-at-biomed.cas.cz Fri Feb 5 07:23:53 2016 13, 45 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 13, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15DNptA021087 13, 45 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 07:23:52 -0600 13, 45 -- X-ASG-Debug-ID: 1454678618-05011e54256712a0001-4CH8be 13, 45 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id inb1JH2j2MV5h0Ez (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 14:23:38 +0100 (CET) 13, 45 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 13, 45 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 13, 45 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 13, 45 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 13, 45 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 45 -- (No client certificate requested) 13, 45 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 388CF2C8070 13, 45 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 14:23:33 +0100 (CET) 13, 45 -- Date: Fri, 5 Feb 2016 14:23:32 +0100 13, 45 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 13, 45 -- To: {microscopy-at-microscopy.com} 13, 45 -- Subject: RE: [Microscopy] viaWWW:Image of speckled mouse muscle. 13, 45 -- Message-ID: {20160205142332.74306056-at-u117ob02} 13, 45 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW:Image of speckled mouse muscle. 13, 45 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 13, 45 -- =?UTF-8?B?xIxS?= 13, 45 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 13, 45 -- MIME-Version: 1.0 13, 45 -- Content-Type: text/plain; charset=UTF-8 13, 45 -- X-IoP-CAS-MailScanner-ID: 388CF2C8070.773A9 13, 45 -- X-IoP-CAS-MailScanner: Processed 13, 45 -- X-Spam-Status: No 13, 45 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 13, 45 -- X-Barracuda-Start-Time: 1454678618 13, 45 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 13, 45 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 13, 45 -- X-Barracuda-Scan-Msg-Size: 7668 13, 45 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 13, 45 -- X-Barracuda-BRTS-Status: 1 13, 45 -- X-Barracuda-Spam-Score: 1.00 13, 45 -- X-Barracuda-Spam-Status: No, SCORE=1.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_RULE_7582B 13, 45 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.26769 13, 45 -- Rule breakdown below 13, 45 -- pts rule name description 13, 45 -- ---- ---------------------- -------------------------------------------------- 13, 45 -- 0.50 BSF_RULE7568M Custom Rule 7568M 13, 45 -- 0.50 BSF_RULE_7582B Custom Rule 7582B 13, 45 -- Content-Transfer-Encoding: 8bit 13, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15DNptA021087 ==============================End of - Headers==============================
Difficult to say when we don't know your primary buffer / fixation protocol, but I would agree that it looks like it may be due to either precipitate from a phosphate buffer, possibly due to insufficient washing between Ga/OSO4 steps, or osmium 'peppering', usually produced with buffer reactions in the osmium steps, or prolonged fixation in osmium.
I used to have similar problems, and resolved them by changing my buffer to HEPES or cacodylate, extra wash steps between the glutaraldehyde and osmium fixes, and secondary fixing in aqueous osmium as opposed to buffered osmium.
Good luck resolving it - let us know how you get on!
Natalie
Miss Natalie Allcock EM Facility Manager
Electron Microscopy Facility, Centre for Core Biotechnology Services, University of Leicester, University Road, Leicester, LE1 7RH, UK * t: +44 (0)116 2523370 e:* nsa2-at-leicester.ac.uk w: http://www2.le.ac.uk/colleges/medbiopsych/research/cbs/eml/electron-microscopy
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-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 04 February 2016 20:05 To: Allcock, Natalie S.
X-from: debrat-at-bcm.edu
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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Email: jmhermel-at-inaf.cnrs-gif.fr Name: Hermel
Organization: CNRS
Title-Subject: [Filtered] Leitz Aristoplan microscope detailled service manual
Message: Hi, I should appreciate any help or link to find a Leitz Aristoplan microscope detailled service manual. This microscope has a problem with de centering of lenses in the microscope stand. Thank you very much.
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Email: mlibbee-at-lbl.gov Name: Marissa Libbee
Organization: LBL
Title-Subject: [Filtered] Open Position at the Molecular Foundry
Message: See description below. For more information, please follow the link http://jobs.lbl.gov/open-positions.html
Molecular Foundry Research Scientist-81646 Organization:MS-Materials Sciences Description
Berkeley Lab is Bringing Science Solutions to the World, and YOU can be a part of it!
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with "excellence." That's why we hire the best - whether in research, finance or other operations. This is a great opportunity to bring your top-notch skills to bear in support of world-class scientific research that addresses national and global challenges!
Position Summary: The National Center for Electron Microscopy (NCEM) facility in the Molecular Foundry at Lawrence Berkeley National Laboratory is seeking a Research Scientist to establish an internationally recognized theory and image simulation research program to advance the state of the art in electron microscopy. The primary responsibilities are: 1) to develop theory and methodology for quantitative comparison between theoretical models and data from advanced electron microscopy instruments, and 2) to lead the development of new techniques and instrumentation for electron scattering. This position will require working on a wide range of collaborative research projects in materials characterization and establishment of an outstanding user program and individual research program.
What You Will Do: Develop image processing and simulation tools to enhance capabilities for quantitative data analysis. Develop an independent research program. Lead or participate in the development of new research directions. Offer computational support for ultrahigh resolution microscopy in aberration-corrected instruments in TEM and STEM. Devise new approaches to overcoming limitations of exit wave reconstruction routines and implement these approaches in NCEM-is user program. Refine theory and simulation for STEM imaging to quantify image analysis. Contribute creatively to the development of techniques for atomic resolution tomography. Establish protocols and work flows for the effective management of large data sets for in-house and remote analysis Participate in the design and implementation of new techniques for imaging of energy-related materials, including enhanced visibility and analysis of light elements, imaging at low dose, analysis of defects, and imaging of thick samples. Provide theoretical insight and support as part of a team to develop new detectors, holography, and other instrumentation and techniques. Lead an effort to help close the gap between experimentally available data and theoretically accessible systems. Be familiar with materials modeling and computational procedures for direct comparison with experimental observations. Modeling approaches could range from macroscopic to atomistic methods, from elastic or thermodynamic to kinetic or first principles techniques. Serve as point of contact for the theory and simulation requirements of users related to nanoscale phenomena accessible to electron microscopy imaging. Oversee operation of image analysis and computer lab at NCEM. Provide expertise in the application of existing commercial software and the development of custom modules and scripts. Serve as scientific contact for user proposals. Critically evaluate scientific proposals for feasibility; design research plans and appropriate microscopy experiments to accomplish specific research objectives. Improve quality of the analysis component of user projects by providing theory and simulation support and collaboration. Work closely with other Molecular Foundry staff scientists. Disseminate research results through publications and presentations at national and international conferences. What Is Required: Proven ability to develop and apply computational methods for image analysis, image simulation and advanced codes for modeling of materials behavior. Excellent scientific publications record in relevant areas Well-developed oral and written communication skills. Ability to work effectively within a team and with various levels of internal and external users and staff. Experience in the management and manipulation of large data sets commensurate with high resolution electron microscopy Ph.D. or equivalent experience in the Physical Sciences or Engineering Strong organizational and time management skills. Ability to manage competing priorities, provide quality work within tight time constraints, and deliver projects on schedule. Additional Desired Qualifications: Experience with computational materials modeling such as density functional theory, molecular dynamics, etc. Strong background of work with experimental data and analysis of images from in situ experiments.
Notes: This is a career appointment.
PLEASE NOTE: This job will close Feb 7, at midnight.
This position requires completion of a background check.
Berkeley Lab addresses the world-is most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab-is scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy-is Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] Research and Development Scientist UK Job Opening
Message: Location: Abingdon, Oxfordshire UK
Gatan is an industry leader in the electron microscopy industry and the Research & Development Scientist will work as part of multidisciplinary teams of scientists and development engineers. The role will provide technical input and deliver innovative technology to new product introductions, meeting project schedules and budgets. There will be a requirement for collaboration across all business functions working closely with the Marketing, Manufacturing and Customer Service teams to assure the success of all new product introductions. The building of relationships and working closely with internal and external customers will be a key requirement.
The prime responsibility will be to engage in product development tasks according to Gatan-is product life cycle (PLC) model, a phased process that includes concept and feasibility, design and development, alpha and beta pilot phases leading to product release. Other responsibilities will involve sustaining engineering for mature and legacy products along with the provision of engineering support to Gatan-is production and customer service teams. The product range and technical challenges are diverse and so the ideal candidate will have a broad range of scientific and engineering skills.
The successful candidate will be cable of working semi-autonomously on multiple tasks and must be capable of providing innovative solutions to technical challenges in high technology product design and development. Equally important will be a keen eye for cost effective solutions that are designed for test, manufacture and service. The role will report directly to the Director of Engineering (UK) and some UK and international travel will be required.
For a full description and application instructions, visit: http://www.gatan.com/company/careers/research-and-development-scientist
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We hope someone on the list has a copy (paper or electronic) of the Oxford INCA User Manual. We were given an 80 page INCA Operator Manual document that provides only a cursory explanation of topics like managing the master standards database. For comparison the Aztec manual is 500 pages. Maybe what we are seeking doesn't exist, but if you do have a manual we'd be happy to pay copying or shipping costs.
Any help you can supply would be appreciated.
Cheers Owen -- Owen P Mills Director, Applied Chemical and Morphological Analysis Laboratory Michigan Technological University 1400 Townsend Dr Houghton, MI 49931 906-369-1875 opmills-at-mtu.edu http://mcff.mtu.edu/acmal/
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Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections
Message: Can anyone recommend a technique for collecting rubber sections during cryo-ultramicrotomy that enables the sections to lie flat (i.e. as wrinkle free as possible) on a TEM support grid? I currently collect my sections off the back of my cryoknife using a sucrose droplet, but as the sections warm coming out of the cryochamber on the droplet they contract and wrinkle before being deposited on a TEM grid (with or without carbon coating). Chamber temperature is -120 C. Any ideas are appreciated. Thank you.
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Email: dalia.yablon-at-surfacechar.com Name: Dalia Yablon
Organization: SurfaceChar
Title-Subject: [Filtered] Upcoming AFM/SPM courses in US, Europe
Message: Dear colleagues,
There are several scanning probe microscopy/atomic force microscopy courses that are happening this spring in both the US and Europe:
April 5-7, 2016 Netherlands: Overview of Scanning Probe Microscopy: A hands-on short course covering the technology, basics of operation, and applications. This course has a March 5, 2016 registration deadline. For course details and registration, go to http://www.surfacechar.com/classesschedule.html
May 10-11, 2016 Boston MA: AFM for Characterization of Polymer Materials. A 2-day hands on course focusing on AFM for polymer applications to study polymer structure, morphology, and materials properties. For course details and registration, go to http://www.afmworkshop.com/characterization-of-polymer-materials-afm-training.html
May 22, 2016 Washington DC: Nanoscale Materials Characterization for the Biomedical and Pharmaceutical Industry. For course details and registration, go to http://techconnectworld.com/World2016/workshops/518.html
Sincerely,
Dalia Yablon, Ph.D. SurfaceChar LLC www.surfacechar.com Dalia.yablon-at-surfacechar.com
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if the wrinkling happens during warming of the droplet, could it be because of the section surface characteristics? Adding salt to the sucrose should help if the surface is charged, multivalent ion salts would be more suited than monovalent. However if the surface is not charged but hydrophobic adding salt would probably increase the wrinkling, and in that case adding a hydrophobic component might help, such as serum albumin. Some detergents can do both.
Good luck!
Jan Leunissen Dunedin, NZ
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I currently collect } my sections off the back of my cryoknife using a sucrose droplet, but as } the sections warm coming out of the cryochamber on the droplet they } contract and wrinkle before being deposited on a TEM grid (with or } without carbon coating). Chamber temperature is -120 C. Any ideas are } appreciated. Thank you. } } Login Host: 199.48.24.10 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } -- } =============================================== } } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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==============================Original Headers============================== 8, 38 -- From leunissen-at-aurion.nl Mon Feb 8 16:06:44 2016 8, 38 -- Received: from nm6-vm7.access.bullet.mail.bf1.yahoo.com (nm6-vm7.access.bullet.mail.bf1.yahoo.com [216.109.114.150]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18M6gFP012755 8, 38 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 16:06:43 -0600 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1454969189; bh=5CcktKX92OvC/tfr4Zhvb2o/3hryqQPIIo0UZ5ZKDDk=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=IM1I0QyuVljo+ccZqg+K37rcVdQl29WJ9vmFsRgw5hVvDM0iWJ6+SWNm1TTSND+CaT8KgB76B2ZFxSL0R0bl0A2G81ROlWQyuAfVpE4oE/E3vH5i/zAlfDS73UX5E/UcmdI6QDOtG2sj9vfyINRMOgdNpwD8c0sguyFTVuvpmzjcO/pDs9rza9dr18JnKmoWp7HqBcaaxpNSfNCIGGy2rKy4A5UErCcsTO2YAPJayvaM5NQeBlWZ2N3/ulKryIVjqPBg+KNBDOh3o9uB8Rj0iuB9/rYmXsCTVPLb+m4o5DYgiEOAs6wOT6TcnEg3kl3BqlXui/vJAaMHP6/KV5E5Xw== 8, 38 -- Received: from [66.196.81.164] by nm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2016 22:06:29 -0000 8, 38 -- Received: from [124.108.96.24] by tm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2016 22:06:29 -0000 8, 38 -- Received: from [127.0.0.1] by omp1001.tnz.mail.aue.yahoo.com with NNFMP; 08 Feb 2016 22:06:28 -0000 8, 38 -- X-Yahoo-Newman-Id: 723320.45878.bm-at-omp1001.tnz.mail.aue.yahoo.com 8, 38 -- Received: (qmail 33870 invoked by uid 1000); 8 Feb 2016 22:06:28 -0000 8, 38 -- Received: from 124.108.96.119 by rel109.mail.aue.yahoo.com with SMTP; Mon, 08 Feb 2016 22:06:28 +0000 8, 38 -- Received: (qmail 30454 invoked from network); 8 Feb 2016 22:06:28 -0000 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1454969188; bh=5CcktKX92OvC/tfr4Zhvb2o/3hryqQPIIo0UZ5ZKDDk=; h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date:Cc:Content-Transfer-Encoding:Message-Id:References:To; b=fiFcNGAtFzcvC1sdBMedTzxosX8EG6cY+3YI964f0JT2geDTyXR3GSixv1w3LGYTF3prV+k4mRW+k8C3SCQgf8eCSaUdy0UkcJX2iZh2wYI4Y3G0PnpHBLURZHVJ1b8etcENiF1d99HI2A2JJRPt/PcRgfMEz/a3qvqb9MnlZ3M= 8, 38 -- X-Yahoo-Newman-Property: ymail-3 8, 38 -- X-YMail-OSG: rq1ahn0VM1kgoVAZKyfIvXbQ_Aqvmwcu8iyyJlT_aTn1_7r 8, 38 -- 7dkY7JiG8Wtvdgs939FPO7d0XoAK1tH.kl2UK64i4axQL4zEX7U_dJgBuL1z 8, 38 -- 4ToOKqlT4iSphgNf5nPpC8idg.PklZliIANd8qPbB_Whhh.O6OAfnz8a9xbC 8, 38 -- tF007IgVq9j95lv2nBEaumiqjIGdaWeH0wBM1hTsFfpY_ko8EIyRA.jMl2nS 8, 38 -- 6mM5MUlu.IcILgop1vS7s9929Cl9jWNNJW0N1xxGvkYCMRvXpTo.6Zie1UWy 8, 38 -- T0QXRv85PUjvVfx6Dz3g89nFtzmMKFqR9BABRiBBUeZX_R6KmcYQrcIa.z0O 8, 38 -- 8UYhreHKLuRS41nY20_BguS0Du.whts1M0.iCOe89Zv0.sRqeWTRTIoVezpA 8, 38 -- eR5e5wCyW_mPuRfCyqkeNDkI1JJNhJqQ5KyMG4z7DQQcU.BHNQN_N07zbBKn 8, 38 -- W6YjLINMnp9r1RkG__CabCeDfvu1zjwDh7ss8KoO1ZqOWzskMjIOsjExv0_w 8, 38 -- b4ok6GhEXr0.hdWn6RrTfD1Jp9yTR7dKYR8Zw5LlP_1_m.ujA 8, 38 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 8, 38 -- Content-Type: text/plain; charset=us-ascii 8, 38 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 8, 38 -- Subject: Re: [Microscopy] viaWWW:TEM - Cryo-ultramicrotomy of rubber sections 8, 38 -- From: Jan Leunissen {leunissen-at-aurion.nl} 8, 38 -- In-Reply-To: {201602082041.u18KfFGe021301-at-ns.microscopy.com} 8, 38 -- Date: Tue, 9 Feb 2016 11:06:26 +1300 8, 38 -- Cc: badamianand-at-bfusa.com 8, 38 -- Message-Id: {D8629196-A927-4DF2-BD24-5C1E3F243E1F-at-aurion.nl} 8, 38 -- References: {201602082041.u18KfFGe021301-at-ns.microscopy.com} 8, 38 -- To: microscopy-at-microscopy.com 8, 38 -- X-Mailer: Apple Mail (2.2104) 8, 38 -- Content-Transfer-Encoding: 8bit 8, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u18M6gFP012755 ==============================End of - Headers==============================
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The hotel reservation link is open and the extended deadline for paper submission is less than a week away. As you (or your students) are making plans to attend the Columbus meetings (July 24-28), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Columbus.
Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up "working" sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.
If anyone would like to participate in the bursary program be sure to check the "I wish to apply for a student bursary" box in section 2 of the registration form (registration opens March 1) and send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
For those 'non-students' volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me. Amanda
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 10, 31 -- From ALawrence-at-i2at.msstate.edu Wed Feb 10 14:04:39 2016 10, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1AK4cDQ026751 10, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 14:04:38 -0600 10, 31 -- Received: from mail06.ad.msstate.edu (mail06.ad.msstate.edu [130.18.230.65]) 10, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id u1AK4OMZ008327 10, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 10, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 14:04:25 -0600 10, 31 -- X-Sender: {} 10, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 10, 31 -- mail06.ad.msstate.edu (2002:8212:e641::8212:e641) with Microsoft SMTP Server 10, 31 -- (TLS) id 15.0.913.22; Wed, 10 Feb 2016 14:04:21 -0600 10, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 10, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 10, 31 -- 15.00.0913.011; Wed, 10 Feb 2016 14:04:21 -0600 10, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 10, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 10, 31 -- Subject: M&M 2016 student and volunteer meeting assistance 10, 31 -- Thread-Topic: M&M 2016 student and volunteer meeting assistance 10, 31 -- Thread-Index: AdFkPWfcCSeNajF7SCqWm3W6g3+d4g== 10, 31 -- Date: Wed, 10 Feb 2016 20:04:20 +0000 10, 31 -- Message-ID: {4ad3a9b7cfc94ddbb127cf560d67af83-at-mail02.ad.msstate.edu} 10, 31 -- Accept-Language: en-US 10, 31 -- Content-Language: en-US 10, 31 -- X-MS-Has-Attach: 10, 31 -- X-MS-TNEF-Correlator: 10, 31 -- x-originating-ip: [130.18.230.93] 10, 31 -- Content-Type: text/plain; charset="us-ascii" 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Transfer-Encoding: 8bit 10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1AK4cDQ026751 ==============================End of - Headers==============================
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Email: alkoh-at-stanford.edu Name: Ai Leen Koh
Organization: Stanford University
Title-Subject: [Filtered] Stanford Imaging Workshop: Advanced applications of high speed imaging in S/TEM
Message: Friday, March 11, 2016 8:30 am - 5:00 pm Stanford University Stanford Nano Shared Facilities 94305-4088 Stanford , CA
Stanford Nano Shared Facilities (SNSF) invites you to attend this unique S/TEM workshop demonstrating the latest high speed imaging technology and applications specifically for in-situ TEM and 4D STEM diffraction.
This comprehensive, full-day workshop will feature lectures by leading experts, open Q&A sessions, and live microscope demonstrations all in the state-of-the-art SNSF research complex. The microscope sessions will be performed on an aberration (image) corrected, monochromated FEI Titan environmental (S)TEM with a OneView in-situ camera.
This workshop is complimentary to all registered and confirmed participants and includes breakfast, lunch and refreshments. Seating is limited.
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Title-Subject: [Filtered] Microscopy Sales Opportunity- Bay Area
Message: Good Afternoon!
We have recently had a world leading microscopy client open up a few microscopy sales opportunities in the greater Bay Area. The ideal candidate would have an expertise in microscopy and some sales experience.
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Email: Linda.Davis-at-Vesuvius.com Name: Linda L Davis
Organization: Vesuvius Refractories R&D USA
Title-Subject: [Filtered] Leitz Wetzlar Orthoplan largefield microscope to give away
Message: Greetings!!
We have an old but excellent research microscope that has been used almost daily here since 1969, by the same microscopist who just retired after 49 years. It is a fantastic scope with a very large footprint. It is a Leitz Wetzlar Orthoplan, large field microscope. It has been serviced by professional companies almost yearly since purchased new in 1969.
We are replacing it, but I hate to just throw this away. The oculars, objectives, accessories may well be something others can use, if not the entire microscope. It is a museum piece. Again, it has not been sitting idle. Please let me know if you want the entire microscope (VERY heavy for shipping, and likely fragile) or any of the parts. It-is located at our R&D site, halfway between Toledo and Cleveland.
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: UNC
Title-Subject: [Filtered] ESEM live image to external computer
Message: Hello Listers There is a video out BNC on the back of the FEI Quanta 200 ESEM. In Low Vac mode I can see the signal when connecting to an external view monitor. The signal is horizontal lines that change in size and number when I change image resolution or scan speed. Also B/C seems to adjust normally. What can I do to make this into a usable image signal to use for internet teaching outreach project? Is there a better way to get the SEM signal to the internet?
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You can use Team Viewer or VNC to directly share desktop of your SEM with remote computer(s).
If you want to grab video from BNC then decent video grabber card and some coding/configuring may be needed. I've used Epiphan PCIe in the past, though not with the FEI E-SEM that you have:
http://www.epiphan.com/products/dvi2pcie-duo/
Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 2/11/2016 12:05 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: wambrose-at-unc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both wambrose-at-unc.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: wambrose-at-unc.edu } Name: Wallace Ambrose } } Organization: UNC } } Title-Subject: [Filtered] ESEM live image to external computer } } Message: Hello Listers } There is a video out BNC on the back of the FEI Quanta 200 } ESEM. In Low Vac mode I can see the signal when connecting to an } external view monitor. The signal is horizontal lines that change in } size and number when I change image resolution or scan speed. Also B/C } seems to adjust normally. What can I do to make this into a usable image } signal to use for internet teaching outreach project? Is there a better } way to get the SEM signal to the internet? } } Login Host: 152.19.204.249 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
==============================Original Headers============================== 7, 33 -- From vray-at-partbeamsystech.com Thu Feb 11 08:23:24 2016 7, 33 -- Received: from nm31-vm2.bullet.mail.bf1.yahoo.com (nm31-vm2.bullet.mail.bf1.yahoo.com [72.30.239.10]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BENO4K008372 7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 08:23:24 -0600 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455200591; bh=a0hht1gkvP2BXihI3+fAlT3Serg9gsbcb8sb6jnlRW0=; h=Subject:To:References:From:Date:In-Reply-To:From:Subject; b=spIVg3Pz6s23ESkgkU/CfkhYIzhTxyuyuhmFDdnzO3/cwsPXIQlQwJ7TX0Jnm7cP76hC0+jwu1VEA6suTi1NyNLy1WrSX/phTmdeCjrfMKXjId+qfZ8YnGRDH7NRElBH0O4Zd1glRPTLYKrYF48Gz5HoDNTpk5wq5FOrckQ7EuDgBFjsBirUXgJtVN5iGcktapp9xHbXLHWT/ELLuErxM7tFLSyoGhlkz2VyUJ/4mLFSc4XHX952t8GaAkC8X8UaQK/60miVprKBaXVG8LgmSzKfJrfwaxsw84p+s7raasBjL/EpNMdX1VNkSMaROvNHWm1cBMOy/CWUZ5jm9DabPg== 7, 33 -- Received: from [98.139.170.182] by nm31.bullet.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:11 -0000 7, 33 -- Received: from [98.139.211.204] by tm25.bullet.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:11 -0000 7, 33 -- Received: from [127.0.0.1] by smtp213.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:10 -0000 7, 33 -- X-Yahoo-Newman-Id: 991022.87580.bm-at-smtp213.mail.bf1.yahoo.com 7, 33 -- X-Yahoo-Newman-Property: ymail-3 7, 33 -- X-YMail-OSG: 9E0eSKoVM1miSumfmEVXNofqt9k2.K51w3WRG_lRjyz3Gym 7, 33 -- R.89fGhBDXHaM22vAiSZHei53AggDUWcKAII8wZG19d3nrPfchxTPXGKhvlm 7, 33 -- OWvLVYSCNW20IZKi9SiQ17q1KMG33niWPqG7xzHoN_t5TNqjVc8ktWnURZ.F 7, 33 -- vYRs9pXJMjOnivgJNtcTwqndKqkxM7Ox2t.YzoOfipcUHMHe66H0Ke8vK4Nw 7, 33 -- VLSaxNAbamrMfHHBqSPICtvyMFU5h3lnpzPz35xGcAIjnC8cuG96pQwy_AP9 7, 33 -- iQcYKqnPKc543BhQ1HP63ZCkeoEKjzPHHkO7_ZVDIz.oPt._GU9JOsHX6w7q 7, 33 -- 5h0sszRs1L3tk1J.UArBU7vblwyrqyi5VB5FBRVIFBdAdk08xPxqxgVfm82s 7, 33 -- Egos3j5jZv7JsulTiTZz8bTlQC8Nd.G1PYiwltdOGjpDBwakw9ms7aeb4_Cf 7, 33 -- 4PltEe0pKxRUZekbEa3btwfvlgjPP7D4wnGKZrEa2naMfqOg.wgE5EUyxR_w 7, 33 -- V0E0udJnYM5NN_WPeEI7hAIv21LKGFcl_tKTgbwdooZmyyu6X7r0DYQ-- 7, 33 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 33 -- Subject: Re: [Microscopy] viaWWW:ESEM live image to external computer 7, 33 -- To: microscopy-at-microscopy.com, wambrose-at-unc.edu 7, 33 -- References: {201602110505.u1B55X86010356-at-ns.microscopy.com} 7, 33 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 33 -- Message-ID: {56BC9950.8020005-at-partbeamsystech.com} 7, 33 -- Date: Thu, 11 Feb 2016 09:23:12 -0500 7, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 7, 33 -- Thunderbird/38.5.1 7, 33 -- MIME-Version: 1.0 7, 33 -- In-Reply-To: {201602110505.u1B55X86010356-at-ns.microscopy.com} 7, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, I am looking for some advice regarding making graphene TEM grids. I've had some success using a resist-free transfer of graphene from copper foil onto a grid. It is the very last step that is most vexing: lifting the graphene covered grid out of the etching solution. The grid is face-down in the solution, floating on a graphene "raft". Picking it up proves problematic. The filter paper pick-up method does not work because the surface tension of the etching solution is such that the grid "runs away" from the paper. I did grab the grids with tweezers after several tries (same issue), but by then damage had been done to the monolayer. One paper suggested draining the etchant away, but then the I am concerned the grid will be face-down on the bottom of the petri dish. Any helpful ideas?
Regards, Larry Scipioni
Regards, Larry
==============================Original Headers============================== 7, 49 -- From les-at-zsgenetics.com Thu Feb 11 10:48:45 2016 7, 49 -- Received: from gateway24.websitewelcome.com (gateway24.websitewelcome.com [192.185.51.162]) 7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BGmjws000462 7, 49 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 10:48:45 -0600 7, 49 -- Received: from cm2.websitewelcome.com (cm2.websitewelcome.com [192.185.178.13]) 7, 49 -- by gateway24.websitewelcome.com (Postfix) with ESMTP id 267E0C0CB16BE 7, 49 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 10:48:32 -0600 (CST) 7, 49 -- Received: from gator2013.hostgator.com ([50.87.144.13]) 7, 49 -- by cm2.websitewelcome.com with 7, 49 -- id H4oW1s00N0HZY1u014oXb3; Thu, 11 Feb 2016 10:48:32 -0600 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; 7, 49 -- d=zsgenetics.com; s=default; h=Content-Transfer-Encoding:Content-Type: 7, 49 -- MIME-Version:Message-ID:Date:Subject:To:From; 7, 49 -- bh=EB0CfrX1XTC7UT3zDSP7gRDPIuRoS/GS8xium3+Msu0=; b=tI4TWfTrlxGC1CT/40b+Pjp9WG 7, 49 -- qKZdKwRgfU1pt4xgX6L+6vvCVV5ZBtEWd9ETsJvzNAuIkIigWm/o1GJ8LsMrz2l5f77I6yU2udKEn 7, 49 -- 0+9BSldYfuKVDVRxVNlvu4kGUXwttp79HrY9P0gks1/AC491xQnlVki7nu014r6JH84w=; 7, 49 -- Received: from zsgenetics03.z.subnet.rcn.com ([146.115.5.114]:50256 helo=LarryPC) 7, 49 -- by gator2013.hostgator.com with esmtpsa (TLSv1.2:DHE-RSA-AES256-GCM-SHA384:256) 7, 49 -- (Exim 4.86) 7, 49 -- (envelope-from {les-at-zsgenetics.com} ) 7, 49 -- id 1aTuPt-000Deu-OP 7, 49 -- for microscopy-at-microscopy.com; Thu, 11 Feb 2016 10:48:29 -0600 7, 49 -- From: {les-at-zsgenetics.com} 7, 49 -- To: {microscopy-at-microscopy.com} 7, 49 -- Subject: graphene TEM grids 7, 49 -- Date: Thu, 11 Feb 2016 11:48:28 -0500 7, 49 -- Message-ID: {008a01d164ec$08d521a0$1a7f64e0$-at-zsgenetics.com} 7, 49 -- MIME-Version: 1.0 7, 49 -- Content-Type: text/plain; 7, 49 -- charset="us-ascii" 7, 49 -- Content-Transfer-Encoding: 7bit 7, 49 -- X-Mailer: Microsoft Outlook 16.0 7, 49 -- Thread-Index: AdFk7AaR4/9UxD7LRIiWEneUlfWzlQ== 7, 49 -- Content-Language: en-us 7, 49 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 7, 49 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 7, 49 -- X-AntiAbuse: Original Domain - microscopy.com 7, 49 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 7, 49 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 7, 49 -- X-BWhitelist: no 7, 49 -- X-Source-IP: 146.115.5.114 7, 49 -- X-Exim-ID: 1aTuPt-000Deu-OP 7, 49 -- X-Source: 7, 49 -- X-Source-Args: 7, 49 -- X-Source-Dir: 7, 49 -- X-Source-Sender: zsgenetics03.z.subnet.rcn.com (LarryPC) [146.115.5.114]:50256 7, 49 -- X-Source-Auth: les-at-zsgenetics.com 7, 49 -- X-Email-Count: 22 7, 49 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
The BNC is definitely the video signal coming out of the Quanta. The question for you is how to get the corresponding X and Y coordinate for the line. You should be able to calculate the line time from the number of pixels and dwell time. You know the number of lines from the pixel count. However, I don't think you will be able to determine where the image starts to get it all in sync.
The D connector next to the BNC is for external control as by an EDS system. I don't know the pin-outs offhand but somebody probably knows them. If you don't have such a system, I don't think I would pursue that route.
I would follow Valery's suggestion of using VNC (or Team Viewer) to monitor the existing screen. You will have to deal with port forwarding since FEI tucks their microscope behind their support computer as a firewall. But they basically do what you need as part of their RAPID support system.
Also, remember that F5 will switch between quadrant and full screen mode. Ctrl-F5 will bring the active quadrant up in full screen on the secondary monitor. I recall there is a way to specify to VNC that that screen should be shared with the rest of the world. It may be that the secondary monitor is what you want to share over the net.
Having said all that, be careful. There is a reason that FEI hid the microscope computer behind a firewall. Make sure your VNC viewers are trustworthy and that they are locked down in view-only mode.
Warren Straszheim
-----Original Message-----
Hi Wallace,
You can use Team Viewer or VNC to directly share desktop of your SEM with remote computer(s).
If you want to grab video from BNC then decent video grabber card and some coding/configuring may be needed. I've used Epiphan PCIe in the past, though not with the FEI E-SEM that you have:
http://www.epiphan.com/products/dvi2pcie-duo/
Valery Ray - also with AIM Lab, UMDCP } X-from: wambrose-at-unc.edu } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying } please copy both wambrose-at-unc.edu as well as the Microscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: wambrose-at-unc.edu } Name: Wallace Ambrose } } Organization: UNC } } Title-Subject: [Filtered] ESEM live image to external computer } } Message: Hello Listers } There is a video out BNC on the back of the FEI Quanta 200 ESEM. In } Low Vac mode I can see the signal when connecting to an external view } monitor. The signal is horizontal lines that change in size and number } when I change image resolution or scan speed. Also B/C seems to adjust } normally. What can I do to make this into a usable image signal to use } for internet teaching outreach project? Is there a better way to get } the SEM signal to the internet? } } Login Host: 152.19.204.249 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------
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From donnedua0-at-gmail.com Thu Feb 11 18:10:19 2016 Return-Path: {donnedua0-at-gmail.com} Received: from gmail.com ([210.126.22.123]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1C0AFJ2027034 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 11 Feb 2016 18:10:17 -0600 Message-ID: {64499D9D.F71C185F-at-gmail.com}
X-from: kaszas.1-at-osu.edu
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Email: kaszas.1-at-osu.edu Name: Andrea Kaszas
Organization: Ohio State University-OARDC
Title-Subject: [Filtered] Protocol for embedding rubber in plastic resin
Message: Anybody has a protocol for embedding rubber samples in plastic resin? Not for cryosectioning. How to prepare the rubber for it, for example hardening the rubber somehow? Any suggestion would be greatly appreciated.
Andrea Kaszas Microscopy technician OSU-OARDC
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Dear Colleagues,
We would like to draw your attention to the upcoming conference on super-resolution light microscopy, hosted at the University of Basel in Switzerland on June 7-11, 2016.
Join world-changing thinkers and innovators from the Biophysical and Life Science communities: Super-resolution imaging methods now can provide spatial resolution that is well below the diffraction limit, approaching virtually molecular resolution. They can be applied to biological samples and provide new and exciting views on the structural organization of cells and the dynamics of biomolecular assemblies on wide timescales. These revolutionary developments come with novel requirements for fluorescent probes, labeling techniques, and data interpretation strategies. Researchers from across the scientific spectrum are involved in designing improved tools and solving previously intractable problems using super-resolution techniques.
We invite you to attend the ,AeoInternational Conference On Nanoscopy 2016,Aeo, which will uniquely and exclusively focus on this topic. Held in Basel, Switzerland 07-10 June 2016, it will bring together an international group of experts to discuss the latest advances and future directions in the field.
Markus Sauer, Conference Chair, University of W/orzburg, Germany
Details and registration are on http://www.icon-europe.org
Speakers include Eric Betzig (Janelia, USA) Lothar Schermelleh (Oxford University, UK) Rainer Heintzmann (University Jena, Germany) Ingo Gregor (University of G/dttingen, Germany) Thomas Huser (University Bielefeld, Germany) Markus Sauer (University W/orzburg, Germany) Adriaan Houtsmuller (Erasmus MC, Netherlands) Jonas Ries (EMBL, Germany) Sjoerd Stallinga (TU Delft, Netherlands) Melike Lakadamyali (ICFO, Spain) Suliana Manley (EPFL, Switzerland) Joerg Bewersdorf (Yale, USA) Katrin Willig (MPI EM, Germany) Christian Eggeling (Oxford University, UK) Ilaria Testa (KTH, Sweden) Martin Booth (Oxford University, UK) Erik Manders (UvA, Netherlands) Edoardo Charbon (TU Delft, Netherlands) Oliver Biehlmaier (University Basel, Switzerland) and others.
Another 12 presentations will be selected from submitted participant abstracts.
On behalf of the organizing committee, Gregor Drummen, Oliver Biehlmaier, Henning Stahlberg, and Manuela Holzer
with best greetings, Henning.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 12, 40 -- From henning.stahlberg-at-unibas.ch Sat Feb 13 04:22:08 2016 12, 40 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1DALurG024691 12, 40 -- for {Microscopy-at-microscopy.com} ; Sat, 13 Feb 2016 04:21:59 -0600 12, 40 -- Received: from localhost (localhost [127.0.0.1]) 12, 40 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 19D5E220164; 12, 40 -- Sat, 13 Feb 2016 11:21:39 +0100 (CET) 12, 40 -- X-Virus-Scanned: amavisd-new at unibas.ch 12, 40 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 12, 40 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 12, 40 -- with LMTP id KA5rUja9CUoh; Sat, 13 Feb 2016 11:21:39 +0100 (CET) 12, 40 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 12, 40 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 097BE22015E; 12, 40 -- Sat, 13 Feb 2016 11:21:39 +0100 (CET) 12, 40 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 12, 40 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 12, 40 -- 14.03.0266.001; Sat, 13 Feb 2016 11:21:38 +0100 12, 40 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 12, 40 -- To: Microscopy {Microscopy-at-microscopy.com} 12, 40 -- CC: Gregor Drummen {gpcdrummen-at-bionano-solutions.de} , 12, 40 -- Oliver Biehlmaier 12, 40 -- {oliver.biehlmaier-at-unibas.ch} , 12, 40 -- Manuela Holzer {manuela.holzer-at-unibas.ch} 12, 40 -- Subject: ICON 2016 - International Conference on Nanoscopy. Basel, June 12, 40 -- 7-11, 2016. Registration now open. 12, 40 -- Thread-Topic: ICON 2016 - International Conference on Nanoscopy. Basel, June 12, 40 -- 7-11, 2016. Registration now open. 12, 40 -- Thread-Index: AQHRZkhS+E7QZ+G7aE25OZYrqmR1Rw== 12, 40 -- Date: Sat, 13 Feb 2016 10:21:38 +0000 12, 40 -- Message-ID: {23434275-3A85-46D6-8712-52E4A27A09B6-at-unibas.ch} 12, 40 -- Accept-Language: en-US, de-CH 12, 40 -- Content-Language: en-US 12, 40 -- X-MS-Has-Attach: 12, 40 -- X-MS-TNEF-Correlator: 12, 40 -- x-originating-ip: [131.152.226.242] 12, 40 -- Content-Type: text/plain; charset="utf-8" 12, 40 -- Content-ID: {31B4E6794420F14FA05CBC4CD0A31CC4-at-zuv.ads.unibas.ch} 12, 40 -- MIME-Version: 1.0 12, 40 -- Content-Transfer-Encoding: 8bit 12, 40 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1DALurG024691 ==============================End of - Headers==============================
Trying to fix one up for personal use. Would be very interested if someone has a manual or can direct me where I might find one. I'm currently following a lead with Pumping Station 1 but looking at other options in case I don't hear back. Thanks!
John
==============================Original Headers============================== 3, 46 -- From johndmcmaster-at-gmail.com Sun Feb 14 23:58:53 2016 3, 46 -- Received: from mail-pa0-f42.google.com (mail-pa0-f42.google.com [209.85.220.42]) 3, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1F5wqvm018810 3, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Feb 2016 23:58:53 -0600 3, 46 -- Received: by mail-pa0-f42.google.com with SMTP id fy10so40839592pac.1 3, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Feb 2016 21:58:39 -0800 (PST) 3, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 46 -- d=gmail.com; s=20120113; 3, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version 3, 46 -- :content-type:content-transfer-encoding; 3, 46 -- bh=YbyWQO2HBy9fF4OCLd6NtFtmMVdarZaqOrQofgfF8yk=; 3, 46 -- b=y2DEAnwRQUR1Ay10UX18b7d4moUB2PGi+CYaqjanIjcpqm6OltC7XYJEVda4AFCuK1 3, 46 -- n3VlbKcncu+Mjp7tQoTroTyrt2O3MsAgCjrapZM6gORHd0W2m87GHcpEA8uhkP+J2i4b 3, 46 -- jpfTra8XrYpGONpsm26HR3cBRb7yfo9pThzRDDxvxo2F1YM8pvAYws/ezgF4IvbwnAk3 3, 46 -- TZiP/R00Yaixrn4Mbj/dqJ/rmFld/+ArvBf8AMiOHqr4BBsJeXH2IldEcuj1uXS16Mku 3, 46 -- IimMk9Ck+rG+oJTson0DY7pp/KQMz1XpK4aeYf2gwqCbws0SVcNxJwUagL0ov1lFkE54 3, 46 -- kpiQ== 3, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 46 -- d=1e100.net; s=20130820; 3, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 3, 46 -- :mime-version:content-type:content-transfer-encoding; 3, 46 -- bh=YbyWQO2HBy9fF4OCLd6NtFtmMVdarZaqOrQofgfF8yk=; 3, 46 -- b=AZ7MHceWb7ouFp2CqIRby8GWD6ZQGsYy6bDiqtSWgB4pEtTYEdG+z757YhmL1wLu8f 3, 46 -- 6rnJ6qmG+0ho6ar880oaYShAhdlYQ8fJRXK2JmdU1gD48S8HfVfHJvNXOFWw2iFbocvF 3, 46 -- oMZc2Kcb0v7Usop68w27Ggw9Q2ZPnUbU4UXLg3mWxvoGwxIQxMV7ffu+r0WF/8Bsl4XT 3, 46 -- w7xhXCgdHk8pItzLpI9MxNiOh67tpHzY1vpzSzm+ZfbnVQ7W/2m4FpJS7xhQ6leVErd0 3, 46 -- HsTSt4B8A3KCTD5eE0t2sswbJFJqP8ayH+Z+vBCJud0LtddXJ9Qvhmg0aGvGK3wVs/au 3, 46 -- PE0w== 3, 46 -- X-Gm-Message-State: AG10YOQliphydnnz9UHSs0/Kt3LS4KorgkuOJFJ2+exiWH5H5tD/XdbgIc5n0ip4zr9tWw== 3, 46 -- X-Received: by 10.66.219.71 with SMTP id pm7mr20818929pac.137.1455515919016; 3, 46 -- Sun, 14 Feb 2016 21:58:39 -0800 (PST) 3, 46 -- Received: from [192.168.4.218] (c-24-6-49-99.hsd1.ca.comcast.net. [24.6.49.99]) 3, 46 -- by smtp.googlemail.com with ESMTPSA id k14sm35302959pfj.0.2016.02.14.21.58.38 3, 46 -- for {Microscopy-at-microscopy.com} 3, 46 -- (version=TLSv1/SSLv3 cipher=OTHER); 3, 46 -- Sun, 14 Feb 2016 21:58:38 -0800 (PST) 3, 46 -- To: Microscopy-at-microscopy.com 3, 46 -- From: John McMaster {johndmcmaster-at-gmail.com} 3, 46 -- Subject: Wanted: Technics Hummer II manual 3, 46 -- Message-ID: {56C1690D.7050304-at-gmail.com} 3, 46 -- Date: Sun, 14 Feb 2016 21:58:37 -0800 3, 46 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 3, 46 -- Thunderbird/38.5.1 3, 46 -- MIME-Version: 1.0 3, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed 3, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: The South Carolina INBRE Program and the USC School of Medicine Instrumentation Resource Facility are pleased to announce the 11th annual workshop on Basic Confocal Microscopy. The Workshop will be held June 13-17, 2016 at the USC School of Medicine in Columbia, SC.
Workshop material is directed towards beginning and intermediate users of confocal microscopes and involves a series of lectures (specimen preparation, labeling strategies, proper set-up of instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point scanning and spinning disk confocal microscopes.
Companies that have provided equipment and applications experts for past workshops include Leica, Nikon, Olympus, Perkin Elmer, Zeiss, Intelligent Imaging Innovations (3i) and Photometrix. Participants will have ample time for hands on use of the instruments and processing of images in Photoshop and AMIRA during the workshop.
Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina.
The $350 tuition covers supplies for processing of specimens, breakfast and lunch for the week, 2 dinners and a reception on Lake Murray.
For registration information please see: http://irf.med.sc.edu/ or contact Anna Harper (Anna.Harper-at-uscmed.sc.edu) or Bob Price (bob.price-at-uscmed.sc.edu)
Bob Price Tel: 803-216-3824 Admin Asst Tel: 803-216-3825 Dept Cell Biology and Anatomy School of Medicine Univ South Carolina 6439 Garner's Ferry Road Columbia, SC 29208
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I am in the process of preparing a grant and I am considering adding about a dozen compound microscopes with phase contrast capabilities 4x-100x objectives. These microscopes would be used in undergraduate teaching labs, so high resolution is unnecessary.
I am unfamiliar with the quality of AmScope microscopes but they have what looks like a decent scope (Model B690B-PL-PCT200INF). We currently have an army of Olympus BH-2s but the number of biology majors has increased significantly, so we are looking for some affordable options.
Does anyone have experience with these microscopes? Any insight would be greatly appreciated!
Thank you,
-Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
anybody ou there who can help me out with an operation manual in PDF on the 5900? I am also looking for a manual concerning the functions which can be remoted on the 5900. Does anybody have experience doing this? What SEM parameters can be read / written...?
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Do any of you have experience with the newer benchtop or desktop SEM's? They seem quite capable and their cost to purchase and maintain, compared to the full scale SEM's, looks attractive. Their advertised performance is appealing. Do they maintain that performance? Do they require much service? Should I have a service contract? Do the accessories function well (e.g. rotating and tilting stage/holder, charge reduction holder)? Is the provided EDS system comprehensive and reliable? Your replies would be greatly appreciated.
Tom Kremer tkremer-at-ipstesting.com
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we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining. Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)? I can't remember the last time I did it but it is a long time ago. I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals. Many thanks in advance.
Regards Stephane
==============================Original Headers============================== 3, 29 -- From nizets2-at-yahoo.com Thu Feb 18 04:08:18 2016 3, 29 -- Received: from nm14-vm0.bullet.mail.bf1.yahoo.com (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IA8HGp022220 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:08:17 -0600 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw== 3, 29 -- Received: from [98.139.215.143] by nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- Received: from [98.139.212.220] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id: 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG: 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka 3, 29 -- 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv 3, 29 -- U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj 3, 29 -- 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ 3, 29 -- m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f 3, 29 -- dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D 3, 29 -- GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT 3, 29 -- XNnKGXA-- 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb 2016 10:08:03 +0000 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To: {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} 3, 29 -- Subject: bias and gain correction on TEM cameras 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References: {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com} ==============================End of - Headers==============================
Hello Stephane, since the blacklevel ("noise") and gain images are a necessary step for getting an artefact-free and evenly background I think you should do this short procedure (depends what version of Analysis or ITEM software you are using) often. When I am installing or updating cameras at the customerYens site I ask for the most used magnfication / spotsize / apertures setting they use and do the correction images with this setting. But: I also know a customer who is doing these image sets prior to each high-res image he uses for publication (makes sense ;-) ).
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 18.02.16 um 11:27 schrieb nizets2-at-yahoo.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining. } Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)? } I can't remember the last time I did it but it is a long time ago. } I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals. } Many thanks in advance. } } Regards } Stephane } } ==============================Original Headers============================== } 3, 29 -- From nizets2-at-yahoo.com Thu Feb 18 04:08:18 2016 } 3, 29 -- Received: from nm14-vm0.bullet.mail.bf1.yahoo.com (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) } 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IA8HGp022220 } 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:08:17 -0600 } 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw== } 3, 29 -- Received: from [98.139.215.143] by nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [98.139.212.220] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- X-Yahoo-Newman-Property: ymail-3 } 3, 29 -- X-Yahoo-Newman-Id: 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com } 3, 29 -- X-YMail-OSG: 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka } 3, 29 -- 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv } 3, 29 -- U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj } 3, 29 -- 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ } 3, 29 -- m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f } 3, 29 -- dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D } 3, 29 -- GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT } 3, 29 -- XNnKGXA-- } 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb 2016 10:08:03 +0000 } 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 (UTC) } 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 3, 29 -- To: {microscopy-at-microscopy.com} } 3, 29 -- Message-ID: {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} } 3, 29 -- Subject: bias and gain correction on TEM cameras } 3, 29 -- MIME-Version: 1.0 } 3, 29 -- Content-Type: text/plain; charset=UTF-8 } 3, 29 -- Content-Transfer-Encoding: 7bit } 3, 29 -- References: {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 25 -- From stefan.diller-at-t-online.de Thu Feb 18 04:40:43 2016 8, 25 -- Received: from mailout01.t-online.de (mailout01.t-online.de [194.25.134.80]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IAefMr004533 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:40:42 -0600 8, 25 -- Received: from fwd11.aul.t-online.de (fwd11.aul.t-online.de [172.20.27.152]) 8, 25 -- by mailout01.t-online.de (Postfix) with SMTP id 3A8F9427EE 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 11:40:27 +0100 (CET) 8, 25 -- Received: from mac-pro.fritz.box (ESUE6aZ-whYwAfSldbPQXTZ55PEafbWxBPK4eG+X+EOX9+4smi90B-sXFMUQ+k6wwJ-at-[91.58.167.160]) by fwd11.t-online.de 8, 25 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 8, 25 -- esmtp id 1aWM0T-1m0f680; Thu, 18 Feb 2016 11:40:21 +0100 8, 25 -- Subject: Re: [Microscopy] bias and gain correction on TEM cameras 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- References: {201602181027.u1IARO2S027682-at-ns.microscopy.com} 8, 25 -- From: Stefan Diller {stefan.diller-at-t-online.de} 8, 25 -- Message-ID: {56C59F94.3010105-at-t-online.de} 8, 25 -- Date: Thu, 18 Feb 2016 11:40:20 +0100 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 8, 25 -- Gecko/20100101 Thunderbird/38.6.0 8, 25 -- MIME-Version: 1.0 8, 25 -- In-Reply-To: {201602181027.u1IARO2S027682-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 8, 25 -- X-ID: ESUE6aZ-whYwAfSldbPQXTZ55PEafbWxBPK4eG+X+EOX9+4smi90B-sXFMUQ+k6wwJ 8, 25 -- X-TOI-MSGID: a7f85412-8967-40de-94d5-f69dc661289f 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IAefMr004533 ==============================End of - Headers==============================
Hi Stephane, We have SIS MegaView II 16 years old, even older then yours Megaview III. Well, we used to record the correction images quite frequently, approximately once per month or two. As the ccd chip and scintillator were getting older, the interval for "shading correction" adjustment had to be shorter. After 15 years, we had to record every week a new set of gain and bias images.
You can quite easily check whether you need to perform new adjustment of "shading correction" or not. 1. Take-off the sample holder out of the column 2. Adjust the illumination on the screen 3. Insert camera 4. Adjust beam intensity to ~50% 5. Take an image. Now measure the histogram of "Gray value distribution" in the recorded image (Main manu: Measure -} Histogram). Look at the course of the histogram line. If it is smooth and the distribution is narrow (~100 for MegaVieew II is OK) then you do not need to adjust "shading correction". Otherwise you should take a new set of gain and bias images.
My best regards
Oldrich
-- Old=oich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization V/ {} de=ask/* 1083 142 20 Prague 4 Czech Republic
On Thu, 18 Feb 2016 04:19:53 -0600, nizets2-at-yahoo.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } we have a SIS Megaview III camera (SIS, then Olympus and now I see it } is EMSIS "again") for over 10 years, a real workhorse, always } performing well without complaining. Because it works so well I tend } to forget about it and now I am just wondering if I need to update } the gain and bias corrections (the correction pictures)? I can't } remember the last time I did it but it is a long time ago. I know } that it is not a lot of work to do it, I just wondered if there is } any reason to do this regularly and if yes, what are the time } intervals. Many thanks in advance. } } Regards } Stephane } } ==============================Original } Headers============================== 3, 29 -- From nizets2-at-yahoo.com } Thu Feb 18 04:08:18 2016 3, 29 -- Received: from } nm14-vm0.bullet.mail.bf1.yahoo.com } (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) 3, 29 -- } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u1IA8HGp022220 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, } 18 Feb 2016 04:08:17 -0600 3, 29 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; } bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; } h=Date:From:Reply-To:To:Subject:References:From:Subject; } b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw== } 3, 29 -- Received: from [98.139.215.143] by } nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [98.139.212.220] by } tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com } with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- } X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id: } 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG: } 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka 3, 29 } -- } 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv } 3, 29 -- } U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj } 3, 29 -- } 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ } 3, 29 -- } m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f } 3, 29 -- } dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D } 3, 29 -- } GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT } 3, 29 -- XNnKGXA-- 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb } 2016 10:08:03 +0000 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 } (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- } Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To: } {microscopy-at-microscopy.com} 3, 29 -- Message-ID: } {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} 3, 29 } -- Subject: bias and gain correction on TEM cameras 3, 29 -- } MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3, } 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References: } {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com} } ==============================End of - } Headers==============================
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==============================Original Headers============================== 12, 48 -- From benada-at-biomed.cas.cz Thu Feb 18 05:39:25 2016 12, 48 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 12, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IBdOm4001310 12, 48 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 05:39:25 -0600 12, 48 -- X-ASG-Debug-ID: 1455795548-05011e54257f2db0001-4CH8be 12, 48 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id tXxpXOM8wjJ82mks (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Thu, 18 Feb 2016 12:39:08 +0100 (CET) 12, 48 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 12, 48 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 12, 48 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 12, 48 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 12, 48 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 12, 48 -- (No client certificate requested) 12, 48 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id DB7002C80E8; 12, 48 -- Thu, 18 Feb 2016 12:39:04 +0100 (CET) 12, 48 -- Date: Thu, 18 Feb 2016 12:39:04 +0100 12, 48 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 12, 48 -- To: nizets2-at-yahoo.com 12, 48 -- Cc: {microscopy-at-microscopy.com} 12, 48 -- Subject: Re: [Microscopy] bias and gain correction on TEM cameras 12, 48 -- Message-ID: {20160218123904.70cccbb3-at-u117ob02} 12, 48 -- X-ASG-Orig-Subj: Re: [Microscopy] bias and gain correction on TEM cameras 12, 48 -- In-Reply-To: {201602181019.u1IAJrKi025081-at-ns.microscopy.com} 12, 48 -- References: {201602181019.u1IAJrKi025081-at-ns.microscopy.com} 12, 48 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 12, 48 -- =?UTF-8?B?xIxS?= 12, 48 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 12, 48 -- MIME-Version: 1.0 12, 48 -- Content-Type: text/plain; charset=UTF-8 12, 48 -- X-IoP-CAS-MailScanner-ID: DB7002C80E8.86B0F 12, 48 -- X-IoP-CAS-MailScanner: Processed 12, 48 -- X-Spam-Status: No 12, 48 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 12, 48 -- X-Barracuda-Start-Time: 1455795548 12, 48 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 12, 48 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 12, 48 -- X-Barracuda-Scan-Msg-Size: 6193 12, 48 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 12, 48 -- X-Barracuda-BRTS-Status: 1 12, 48 -- X-Barracuda-Spam-Score: 0.50 12, 48 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_SC0_MISMATCH_TO 12, 48 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.27131 12, 48 -- Rule breakdown below 12, 48 -- pts rule name description 12, 48 -- ---- ---------------------- -------------------------------------------------- 12, 48 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header 12, 48 -- 0.50 BSF_RULE7568M Custom Rule 7568M 12, 48 -- Content-Transfer-Encoding: 8bit 12, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IBdOm4001310 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University at Kingston
Title-Subject: [Filtered] How batch convert emi to other format
Message: Dear all, We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I can open them in TIA software, and can only export them one by one. That is quite annoying. Is there anybody who has any idea how to batch convert the images?
Thanks Hongbing Yu
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Email: mlibbee-at-lbl.gov Name: Marissa Libbee
Organization: LBL
Title-Subject: [Filtered] Attn: LaB6 cathode/e-gun mfrs and vendors
Message: I'm interested in tracking down mfrs and vendors of anything LaB6 related. Please contact me offline with company names and preferably, a contact name within the company. Thanks!
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Title-Subject: [Filtered] EM Connectome Annotation Team Manager (362-908 )
Message: The Janelia Research Campus of the Howard Hughes Medical Institute (Janelia) is using high-throughput electron microscopy (EM) to completely image multiple brains and nervous systems of the fruit fly, Drosophila melanogaster. Janelia scientists will utilize these massive data sets to reconstruct neuronal circuits, ultimately culminating in the complete connectome-oor wiring diagram-oof the fly brain. The connectome will provide the most complete anatomical description of a complex nervous system to date in an organism uniquely suited to combine this anatomical information with functional imaging, electrophysiology, quantitative behavioral assays, precise genetic manipulation of identified cell types and computational modeling. Thus we expect these circuit-level reconstructions to inform specific biological experiments, enabling dramatic leaps in our understanding of brain function.
Janelia is seeking a talented individual to assemble, train, and lead a group of approximately ten neuron tracing staff, with potential for a significantly larger effort based on the established success of the initial team. In order to be successful, the manager must have research experience in neuroanatomy, preferably in the application of electron microscopy to elucidate neurobiological function. Knowledge of Drosophila biology and/or software for reconstructing objects from serial section images is strongly desired. The manager will collaborate with scientists (at Janelia and elsewhere) whose research is targeted toward understanding of specific circuits and functions in the fly brain, guiding their team of highly trained specialists to identify, trace, and annotate specific collections of neurons from the vast data being produced. The manager will also collaborate closely with the project teams producing the EM data and developing the algorithms and tools that enable the work of their team. Thus, the ideal candidate for this position must have a neuroscience background, the ability to teach scientific concepts, and experience in supervising and leading the work of others in a dynamic setting. The candidate must further possess an innate sense of pace and urgency, and excel at working in a team science environment to achieve challenging goals, prioritizing multiple assignments, and communicating clearly to a broad group of collaborators. Experience in supervising and leading the work of others is desired but not strictly required.
HHMI is an Equal Opportunity Employer.
Please apply online at https://hhmi-openhire.silkroad.com/epostings/index.cfm?fuseaction=app.jobinfo&jobid=362&company_id=16908&version=1&source=ONLINE&jobOwner=992274&aid=1.
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TIA folder export: - Open menu Tools\Components. - Click ,AeuInstall,Aeu. - Type ,Aeufolderexport.wsc,Aeu. - ,Aeuok". Now that this component is installed, there will be an additional menu in the processing tasks. Check settings and hit Export.
Best,
Wim Hagen EMBL Heidelberg
} On Feb 19, 2016, at 1:58 AM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: 12hy1-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: 12hy1-at-queensu.ca } Name: Hongbing Yu } } Organization: Queen's University at Kingston } } Title-Subject: [Filtered] How batch convert emi to other format } } Message: Dear all, } We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I } can open them in TIA software, and can only export them one by one. That is quite annoying. Is there } anybody who has any idea how to batch convert the images? } } Thanks } Hongbing Yu } } } Login Host: 130.15.34.113 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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==============================Original Headers============================== 7, 27 -- From wim.hagen-at-me.com Thu Feb 18 20:53:36 2016 7, 27 -- Received: from pv33p04im-asmtp001.me.com (pv33p04im-asmtp001.me.com [17.143.181.10]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J2raff023321 7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 20:53:36 -0600 7, 27 -- Received: from wim-hagens-macbook-pro.local.pool.embl.de 7, 27 -- (unknown [194.94.44.220]) by pv33p04im-asmtp001.me.com 7, 27 -- (Oracle Communications Messaging Server 7.0.5.36.0 64bit (built Sep 8 2015)) 7, 27 -- with ESMTPSA id {0O2R0085PXCMH640-at-pv33p04im-asmtp001.me.com} for 7, 27 -- Microscopy-at-microscopy.com; Fri, 19 Feb 2016 02:53:14 +0000 (GMT) 7, 27 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, 7, 27 -- definitions=2016-02-19_02:,, signatures=0 7, 27 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 7, 27 -- clxscore=1011 suspectscore=2 malwarescore=0 phishscore=0 adultscore=0 7, 27 -- bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 7, 27 -- engine=8.0.1-1510270003 definitions=main-1602190039 7, 27 -- Content-type: text/plain; charset=utf-8 7, 27 -- MIME-version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 7, 27 -- Subject: Re: [Microscopy] viaWWW:How batch convert emi to other format 7, 27 -- From: Wim Hagen {wim.hagen-at-me.com} 7, 27 -- In-reply-to: {201602190058.u1J0wuXt027832-at-ns.microscopy.com} 7, 27 -- Date: Fri, 19 Feb 2016 03:53:09 +0100 7, 27 -- Message-id: {EA4C4200-6FBC-4DD6-9DC2-B4F92F0BF494-at-me.com} 7, 27 -- References: {201602190058.u1J0wuXt027832-at-ns.microscopy.com} 7, 27 -- To: 12hy1-at-queensu.ca, Microscopy-at-microscopy.com 7, 27 -- X-Mailer: Apple Mail (2.2104) 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1J2raff023321 ==============================End of - Headers==============================
I am need of some experience here. Has anyone played with UA replacements such samarium and gadolinium triacetate for en bloc staining? How does this compare in performance, permeation, precipitation etc? Is it an acceptable alternative for all tissues or just selected ones. I am aware of many papers on the mater, and my own dabbling but I tend to find the microscopy list opinion a little easier to swallow.
Thanks in advance J
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After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes.-* These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches.-* This has made staining and coverslipping very difficult, even with-* hood.-*-*-*Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount?-* thanks very much!
Daniel Blackburn Biology, Trinity College (Hartford CT) -*
==============================Original Headers============================== 4, 29 -- From dblackburn2000-at-yahoo.com Sat Feb 20 10:55:19 2016 4, 29 -- Received: from nm13-vm0.bullet.mail.bf1.yahoo.com (nm13-vm0.bullet.mail.bf1.yahoo.com [98.139.213.79]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1KGtJqN017594 4, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 20 Feb 2016 10:55:19 -0600 4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455987305; bh=GYAjj76GzomM7vop6UUnJyXkz0u+wZUkwa3SDd0g15A=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=TPxqPNC/tvQBHsTKLGuQ51Jxg7PrRk1Z77IjDYAtNrZPBfyLnjxprl57gqSAmcE6keQ080eKlTjoR/clH40sa3IPkQqVdl3HUZaugXs9TzpNOF+PJyPx7Ey2G68vm51CkrE0pfnoYku9ZaNs+8dasZxwEoJOt5N4tyekwNeyVH4GCpXRv4EFgmxNTtJtzt5Z6fJ9cGdjkoEooZibBz86jBWN70VTdga00AoibIBFXh/0g4qvxNfJKsxYB/XhmCgB/ZG4fK8She7vD2xu2k/W1HqNDBSK77t/7eLBoeuKid0158KLd6UXCr9j7zSErsJ0xwuMBw9LmRrmi9iC10fEHg== 4, 29 -- Received: from [66.196.81.170] by nm13.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000 4, 29 -- Received: from [98.139.212.222] by tm16.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000 4, 29 -- Received: from [127.0.0.1] by omp1031.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000 4, 29 -- X-Yahoo-Newman-Property: ymail-3 4, 29 -- X-Yahoo-Newman-Id: 532123.73121.bm-at-omp1031.mail.bf1.yahoo.com 4, 29 -- X-YMail-OSG: pQ6H50EVM1mOAEYBONpigG3IZYomqCLazwII_MkU0fjcMa3_mrHLGMVY9za3Cb5 4, 29 -- rtfU9TxN46HHIjZZmoXVODUSN2PFdj3kF9Ae_nSuAc71MZ2azIzwmB4U4FJaEynMUv_j1mX4wReD 4, 29 -- mREMmAAZnMyIZTZuaQekhHvng_BLwBMnJ5vL9WPtVQxYrY2f1mGvDO4yzQZ31iy_AKLJtY9aZ2hV 4, 29 -- CeSPnr1ZrqqTnIR1XTfmWUi4OObTKxkXdcRKYUc0J7cKTEjtonO5nAK5yoXFFcZrxWTjUHSaQl8q 4, 29 -- 5E2S3qBWMCAyAOkd8im.uNmteAlj7usrcZXcQvG4cRrGBi8zNdGCptRXt2.2knijRUVID_.fcHWl 4, 29 -- 4XVdFdmAJirUe0hNywHM6LYT87WdteIDhiNyWHloS5g3DIr8jFKjILiRsuMQqSAiR.ERWgXqlZvj 4, 29 -- rvi0eS7x7hsidXf0j7fHi6aGnkElT8D8cxLc0Ivs2n9rouVgLONbz 4, 29 -- Received: by 76.13.27.50; Sat, 20 Feb 2016 16:55:05 +0000 4, 29 -- Date: Sat, 20 Feb 2016 16:55:04 +0000 (UTC) 4, 29 -- From: daniel blackburn {dblackburn2000-at-yahoo.com} 4, 29 -- Reply-To: daniel blackburn {dblackburn2000-at-yahoo.com} 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- Message-ID: {370209020.7409633.1455987304853.JavaMail.yahoo-at-mail.yahoo.com} 4, 29 -- Subject: question - Allergy to histological solvents? 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset=UTF-8 4, 29 -- References: {370209020.7409633.1455987304853.JavaMail.yahoo.ref-at-mail.yahoo.com} 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1KGtJqN017594 ==============================End of - Headers==============================
I assume you're doing these steps in a fume hood. One thing we've found for people who are sensitive to solvent fumes are charcoal-lined dust masks. Search amazon.com for "charcoal filter masks". We use the 3M type (but NOT for the prices here - we got ours through Fisher Scientific). So far, they've worked fine.
Looks like you've already tried the various xylene replacements.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
________________________________________ X-from: dblackburn2000-at-yahoo.com [dblackburn2000-at-yahoo.com] Sent: Saturday, February 20, 2016 12:16 PM To: Oshel, Philip Eugene
Hello
After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes. These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches. This has made staining and coverslipping very difficult, even with hood. Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount? thanks very much!
Daniel Blackburn Biology, Trinity College (Hartford CT)
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==============================Original Headers============================== 14, 35 -- From oshel1pe-at-cmich.edu Sun Feb 21 09:20:13 2016 14, 35 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 14, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1LFKCwi014223 14, 35 -- for {microscopy-at-microscopy.com} ; Sun, 21 Feb 2016 09:20:13 -0600 14, 35 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 14, 35 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u1LFJvtI005485; 14, 35 -- Sun, 21 Feb 2016 10:19:57 -0500 14, 35 -- Received: from cmail106.central.cmich.local ([fe80::a8:3e31:c722:be0a]) by 14, 35 -- cas2.central.cmich.local ([fe80::c44b:39ae:767:cab7%10]) with mapi id 14, 35 -- 14.03.0248.002; Sun, 21 Feb 2016 10:19:57 -0500 14, 35 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 14, 35 -- To: "dblackburn2000-at-yahoo.com" {dblackburn2000-at-yahoo.com} 14, 35 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 35 -- Subject: RE: [Microscopy] question - Allergy to histological solvents? 14, 35 -- Thread-Topic: [Microscopy] question - Allergy to histological solvents? 14, 35 -- Thread-Index: AQHRbAJzvIVVjB+UVkiX60gI5zJcWJ82nTv9 14, 35 -- Date: Sun, 21 Feb 2016 15:19:57 +0000 14, 35 -- Message-ID: {FB5ED57CF3415D4F8C5F93F1DE5468732FCE720F-at-cmail106.central.cmich.local} 14, 35 -- References: {201602201716.u1KHGmXC002359-at-ns.microscopy.com} 14, 35 -- In-Reply-To: {201602201716.u1KHGmXC002359-at-ns.microscopy.com} 14, 35 -- Accept-Language: en-US 14, 35 -- Content-Language: en-US 14, 35 -- X-MS-Has-Attach: 14, 35 -- X-MS-TNEF-Correlator: 14, 35 -- x-originating-ip: [141.209.2.100] 14, 35 -- Content-Type: text/plain; charset="us-ascii" 14, 35 -- MIME-Version: 1.0 14, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 14, 35 -- X-Spam-Score: -6.00 () [Hold at 6.00] L_CTCMUM,L_EXCH_MF,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 14, 35 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 14, 35 -- X-CanItPRO-Stream: default 14, 35 -- X-Canit-Stats-ID: 05Ql3jVMv - 2337180cc265 - 20160221 14, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 14, 35 -- Content-Transfer-Encoding: 8bit 14, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1LFKCwi014223 ==============================End of - Headers==============================
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A serious problem. By listing, I have come with suggestions. I have long used a Gum Damar formulation that I cannot offer, because I have prepared it in xylene.
NOTE: for those who might be interested - or users of Damar - I have a liter that I will NEVER use more than a few ml's over my reasonably extended age - now 76. Further, I don't do paraffin any more.
The suggestions:
If you can arrive at a solvent for Damar that is miscible with xylene/toluene, then it would be possible that you could prepare your own Damar. NOTE: Damar applied in the 1960's still have no sign of drying/cracking/crystalizing. Source: http://www.eco-house.com/shop/950-damar-resin-crystals-graded/
You might test a hydrophilic mountant. Source: https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx
If your allergy is broad-band, then you may have to resort to the latter. If it is more specific - just for those you mentioned - then your options might include Canada Balsam that is very natural, but also often found with benzene or xylene or toulene. I have some that will never be used as it requires some solvent that might not raise a sneeze.
Email me directly if you wish to discuss further,
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu
________________________________________ X-from: daniel blackburn via Histonet {histonet-at-lists.utsouthwestern.edu} Sent: Saturday, February 20, 2016 2:44 PM To: Histonet-at-lists.utsouthwestern.edu
X-from: mp76ers-at-gmail.com
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Email: mp76ers-at-gmail.com Name: Mojahed
Organization: MERC
Title-Subject: [Filtered] Cambridge S360 SEM Computer Error
Message: Hi to all, I have a problem with the microcomputer (SBC) of an old s360 SEM. It's model is PME 68-2 (or PME 68-12). It has s1-s8 LEDs on its front panel for error checking. Now s5 LED indicate en error. Does anyone have its manual or know what does it mean?
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I would like to hear from anyone out there who is using FEI's TeneoVS SEM. I want to know your experiences with it and I would like to get a sample run as a demo, we can cover any associated costs.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Mon Feb 22 08:30:10 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MEUA1r001242 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:30:10 -0600 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id EAA6F5C5E75 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:07:31 -0600 (CST) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 02E195C5E6A 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:07:31 -0600 (CST) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Mon, 22 Feb 2016 08:29:55 -0600 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Users of FEI TeneoVS SEM 7, 37 -- Thread-Topic: Users of FEI TeneoVS SEM 7, 37 -- Thread-Index: AdFtfSnkLKYP9x9wRmufl/4nk4jZUQ== 7, 37 -- Date: Mon, 22 Feb 2016 14:29:53 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE854050-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 22c5e17d-fb11-48c5-a02c-43161552b8cd 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 668ad697-ab40-4dca-8a37-267e6a56aa92 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1MEUA1r001242 ==============================End of - Headers==============================
Unfortunately, you,Aeove probably lost your sense of smell and taste if you,Aeove become that sensitized. I would suggest the quickest remedy may be using a half-mask respirator with organic vapor filter cartridges, even though you may be using a fume hood. I think paint respirators may work since they filter organics/solvents/paints. Make sure it fits correctly too. It won't do to have a proper filtering assembly if it doesn't fit correctly! Good luck. ~Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W. Wiggins, E.M. Pathology Specialist CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron Microscopy, LLSPT, A828 8700 Beverly Blvd. Los Angeles, CA 90048-1865 OAess310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) Winston.Wiggins-at-CSHS.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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==============================Original Headers============================== 5, 50 -- From winston.wiggins-at-cshs.org Mon Feb 22 10:09:23 2016 5, 50 -- Received: from espxlsmg04.csmc.edu (mx4.csmc.edu [192.231.86.201]) 5, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MG9Mof024718 5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 10:09:23 -0600 5, 50 -- X-AuditID: 0a200141-f790b6d00000272b-9e-56cb32a01a48 5, 50 -- Received: from CSHSMSGZIX04.CSMC.EDU (cshsmsgzix04.csmc.edu [10.17.158.163]) 5, 50 -- by espxlsmg04.csmc.edu (CS) with SMTP id 7C.A0.10027.0A23BC65; Mon, 22 Feb 2016 08:09:04 -0800 (PST) 5, 50 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 5, 50 -- by Outbound.cshs.org (Proprietary) with SMTP id 0B0DC7203FD 5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:04:56 -0800 (PST) 5, 50 -- Received: from ESPWMSGHUB05.CSMC.EDU (espwmsghub05.csmc.edu [10.17.239.90]) 5, 50 -- by CSHSMSGZIX04.CSMC.EDU (Proprietary) with ESMTP id D42CD7203FB 5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:04:55 -0800 (PST) 5, 50 -- Received: from CSHSMSGMBX01.CSMC.EDU ([169.254.3.125]) by 5, 50 -- ESPWMSGHUB05.CSMC.EDU ([10.17.239.90]) with mapi id 14.03.0235.001; Mon, 22 5, 50 -- Feb 2016 08:09:08 -0800 5, 50 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 5, 50 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 5, 50 -- Subject: RE: question - Allergy to histological solvents? 5, 50 -- Thread-Topic: RE: question - Allergy to histological solvents? 5, 50 -- Thread-Index: AdFtiEeekRFJ5XMDSxmYMvDNpL2XNw== 5, 50 -- Date: Mon, 22 Feb 2016 16:09:07 +0000 5, 50 -- Message-ID: {77186365EA63114782772AB6D4940EEE7A76049A-at-cshsmsgmbx01.CSMC.EDU} 5, 50 -- Accept-Language: en-US 5, 50 -- Content-Language: en-US 5, 50 -- X-MS-Has-Attach: 5, 50 -- X-MS-TNEF-Correlator: 5, 50 -- x-originating-ip: [166.124.28.190] 5, 50 -- Content-Type: text/plain; charset="utf-8" 5, 50 -- MIME-Version: 1.0 5, 50 -- X-VPM-MSG-ID: ecee1c8c-3893-4e58-a917-854ca910f97f 5, 50 -- X-VPM-HOST: CSHSMSGZIX04.CSMC.EDU 5, 50 -- X-VPM-GROUP-ID: f17f7004-8acd-4c98-922c-6736a276b9ca 5, 50 -- X-VPM-ENC-REGIME: Plaintext 5, 50 -- X-VPM-CERT-FLAG: 0 5, 50 -- X-VPM-IS-HYBRID: 0 5, 50 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFtrKIsWRmVeSWpSXmKPExsXCJThvse4Co9NhBu+mC1j8OdzA7sDo8bH7 5, 50 -- NVMAYxSXTUpqTmZZapG+XQJXxr5nU9gKNvFVfJvv18DYwNfFyMEhIWAi8XRSaBcjJ5ApJnHh 5, 50 -- 3nq2LkYuDiGBzYwSKw//ZoRwtjBKnHm2HMq5zijRe2obE4SzkFHiRd9qJpBRbALGEu/W5IKM 5, 50 -- EhGwlfh24Q4TiC0sYCkx/+kmRoi4ncS5viUsELaexKbJp8DiLAKqEidfHmEFsXkFfCT6bnYx 5, 50 -- g9iMQCd9P7UGbA6zgLjErSfzmSBOFZBYsuc8M4QtKvHy8T9WCFtJYt36i2wg5zALaEqs36UP 5, 50 -- 0aooMaX7ITvEeEGJkzOfsECUG0nseHmRHcJWlNhz7h8bhG0i8X7jSai4tMSqF3NZIIElJHH7 5, 50 -- lBZEWEji7Il7rBMYpWchOW4WwuJZSBbPQrJ4ASPLKkbh1OKCipzi3HQDE73k4txkvdSU0k2M 5, 50 -- oMhUYHTcwTjhku0hRgEORiUeXgPOU2FCrIllxZW5hxglOJiVRHjrpE6HCfGmJFZWpRblxxeV 5, 50 -- 5qQWH2KU5mBREuft3nw8TEggPbEkNTs1tSC1CCbLxMEp1cDYXx7+Vfbnh9k++qw24QziSvn/ 5, 50 -- Ju3defxFk9rOP50n6wqV5kvVeUZYF5VccLZY7nGmZdIWrmVRWc6xpSJMl19tXiKu/N09JDrx 5, 50 -- +j8RqwAvo3tznX7xC3zQ4pQ39Xgj+f3C9nY3uWnnSlWXK6gdkVdhtPW68HtNk1XohFU5vRNk 5, 50 -- KjS3TY1/+VmJpTgj0VCLuag4EQAiRscByAIAAA== 5, 50 -- Content-Transfer-Encoding: 8bit 5, 50 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1MG9Mof024718 ==============================End of - Headers==============================
Mississippi State University's College of Engineering will be hosting a Research Experience for Undergraduates May 31-Aug. 6.
Research will be in Materials Science and Engineering and will include topics such as biomaterials, nanoparticles, composites & polymers, and nanocrystalline materials. The program will also include professional and development seminars, industry site visits, and GRE preparation workshops.
The REU includes a weekly stipend, on-campus housing, and a meal allowance. For more information and/or to apply on-line please visit the college of engineering website: www.bagley.msstate.edu/REU/.
Amanda Lawrence Outreach Coordinator/Research Associate Institute for Imaging and Analytical Technologies Mississippi State University
==============================Original Headers============================== 7, 33 -- From ALawrence-at-i2at.msstate.edu Mon Feb 22 16:04:05 2016 7, 33 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MM45OT025147 7, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 16:04:05 -0600 7, 33 -- Received: from mail06.ad.msstate.edu (mail06.ad.msstate.edu [130.18.230.65]) 7, 33 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id u1MM3pgk011042 7, 33 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 7, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 16:03:51 -0600 7, 33 -- X-Sender: {} 7, 33 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 7, 33 -- mail06.ad.msstate.edu (2002:8212:e641::8212:e641) with Microsoft SMTP Server 7, 33 -- (TLS) id 15.0.913.22; Mon, 22 Feb 2016 16:03:50 -0600 7, 33 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 7, 33 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 7, 33 -- 15.00.0913.011; Mon, 22 Feb 2016 16:03:50 -0600 7, 33 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 7, 33 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 7, 33 -- Subject: summer research experience for engineering and materials science 7, 33 -- undergraduates 7, 33 -- Thread-Topic: summer research experience for engineering and materials 7, 33 -- science undergraduates 7, 33 -- Thread-Index: AdFtvC1M/yUDJOBSSVSKwPnrpzqTTg== 7, 33 -- Date: Mon, 22 Feb 2016 22:03:50 +0000 7, 33 -- Message-ID: {485c84d1773740c2856dbc8514520eb2-at-mail02.ad.msstate.edu} 7, 33 -- Accept-Language: en-US 7, 33 -- Content-Language: en-US 7, 33 -- X-MS-Has-Attach: 7, 33 -- X-MS-TNEF-Correlator: 7, 33 -- x-originating-ip: [130.18.230.93] 7, 33 -- Content-Type: text/plain; charset="us-ascii" 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1MM45OT025147 ==============================End of - Headers==============================
From advertise.bz222dcedu-at-gmail.com Wed Feb 24 00:57:18 2016 Return-Path: {advertise.bz222dcedu-at-gmail.com} Received: from gmail.com ([121.78.112.171]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1O6vFHM030289 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 24 Feb 2016 00:57:16 -0600 Message-ID: {2EC237BE.15C3727C-at-gmail.com}
In 1921, Ms.Valencia Arton took for her second term, summer quarter a course called Textiles at the University of Chicago. She constructed a lab book consisting of experiments, typed and long hand notes and samples of fabric before and after testing.
I've found her notebook over 30 years ago in a used bookstore thinking, "What a source of old fabric samples and fibers" but I never did anything with it.
I'm sure Ms. Arton is long past caring, but I can't help but think some microscopist could find a publication in these pages, if they look hard enough. Valencia would probably enjoy that.
The book is free to anyone who would want it, no strings attached. She does mention the microscope and it is an interesting look at the analytical fiber and fabric procedures of 1921.
Contact me if you have question or need more detail.........
Frank ARDL
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==============================Original Headers============================== 10, 27 -- From frank_karl-at-ardl.com Wed Feb 24 07:07:25 2016 10, 27 -- Received: from cal1-mh746b.smtproutes.com (cal1-mh746b.smtproutes.com [208.70.89.157]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OD7OdY006599 10, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 07:07:24 -0600 10, 27 -- X-Katharion-ID: 1456319214.50048.cal1-mh746 10, 27 -- Received: from mail.ardl.com ([98.100.51.26]) by 10, 27 -- cal1-mh746.smtproutes.com [(192.69.16.68)] with ESMTP via TCP 10, 27 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 24 Feb 2016 13:06:54 +0000 10, 27 -- Received: from exchange2k7.ad.ardl.com ([fe80::596e:af10:621c:b9f8]) by 10, 27 -- exchange2k7.ad.ardl.com ([fe80::596e:af10:621c:b9f8%14]) with mapi; Wed, 24 10, 27 -- Feb 2016 08:06:54 -0500 10, 27 -- From: Frank Karl {frank_karl-at-ardl.com} 10, 27 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 10, 27 -- Date: Wed, 24 Feb 2016 08:06:53 -0500 10, 27 -- Subject: Fiber and Fabric 10, 27 -- Thread-Topic: Fiber and Fabric 10, 27 -- Thread-Index: AdFvBDt1tIQveEdOS+ORmMZWmyaU5A== 10, 27 -- Message-ID: {0445DA8FFC235F4CBDAF9BACCF79E12108C792C0-at-exchange2k7.ad.ardl.com} 10, 27 -- Accept-Language: en-US 10, 27 -- Content-Language: en-US 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- acceptlanguage: en-US 10, 27 -- Content-Type: text/plain; charset="us-ascii" 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1OD7OdY006599 ==============================End of - Headers==============================
The Organizing Committee of Ultrapath XVIII (http://congress.ultrapathxviii.org) has extended the deadline for abstract submission to March 24, 2016. Your work is very important for the Society and your contribution to the Congress will help all of us to keep improving and spread the word about the continued importance of Ultrastructural Pathology.
With kind regards,
From the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 6, 46 -- From apamatos-at-gmail.com Wed Feb 24 08:08:11 2016 6, 46 -- Received: from mail-wm0-f52.google.com (mail-wm0-f52.google.com [74.125.82.52]) 6, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OE8BfF028573 6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 08:08:11 -0600 6, 46 -- Received: by mail-wm0-f52.google.com with SMTP id g62so6243159wme.0 6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 06:07:57 -0800 (PST) 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=gmail.com; s=20120113; 6, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version 6, 46 -- :content-transfer-encoding; 6, 46 -- bh=jGwYeYU2P9NVDSHXIC/ikbUi66LcT+w0CW3CNixU668=; 6, 46 -- b=AyWnZTkk64GX2E3ViGo4aeetpSqKRZBYdShbpzeUvbrrLEnLqFCZ8CyiDB2wmIZIzl 6, 46 -- 5VLSMTW2ztw8zgVsCZAjXDC+q2Uh6+j1qOseUMeO19zhZKAIWM19oqLR5Bnlvym07Fzs 6, 46 -- XUj4E46nj4nHpWiVWpVvmASogYRUGtLjyppPVZ1FufqulY9odLuIagYASTAwHDHuKHZd 6, 46 -- tglqfmWGzHUcB5Oqq6uB2GruiClY9DfkhG8tM43YJ0C9Zgi0L3nMpm0rLH7CGrATXndm 6, 46 -- qkld+/dCBHFU3J+paFA6OcZtAeVj80Ot+fPWqKF5dqGz/vBXIYG8q7y8NUmzbGCpCjB2 6, 46 -- LZoQ== 6, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=1e100.net; s=20130820; 6, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 6, 46 -- :mime-version:content-transfer-encoding; 6, 46 -- bh=jGwYeYU2P9NVDSHXIC/ikbUi66LcT+w0CW3CNixU668=; 6, 46 -- b=RwpqrBKKMRzYx+dbOnsaxl+pkpC57UpteKuRJSo/ikl//4ZdxV9TWcdRPX5+qqitm+ 6, 46 -- In+biXygyuua0qWor3+IInJgcTobaGhjDVoE04iKr/cEtxxBbCO2ZGctbqM5f2KXwo1r 6, 46 -- digaDiPDAw9493jqxOMvXR6ZAIKSav5DVZZ+XH83+6RSejSeYkX0t38rH6VE3KhdN2sL 6, 46 -- hL/K5R1zCaY+/Et3oEBzUSYyIhf/reJXhz1az5E/4rj+C+IMrpCvuKTX30eOhnh2HNYA 6, 46 -- SGmX/ELYZp5NBY3cLqYNkAKNVCc/NTT/1C57e6UBmJEj8Wuyi6rIxP3a1KwubHcmEARJ 6, 46 -- 1K0Q== 6, 46 -- X-Gm-Message-State: AG10YOS50eWzhmbrpT34bvFzMofZ1s8ECgiP2LfRp851Ipps+eHzjsHHK2gBtLW91GMe3A== 6, 46 -- X-Received: by 10.28.14.140 with SMTP id 134mr23384508wmo.39.1456322876619; 6, 46 -- Wed, 24 Feb 2016 06:07:56 -0800 (PST) 6, 46 -- Received: from ?IPv6:2001:8a0:f946:ec01:4261:86ff:fe2b:8e36? ([2001:8a0:f946:ec01:4261:86ff:fe2b:8e36]) 6, 46 -- by smtp.gmail.com with ESMTPSA id gb9sm3204146wjb.26.2016.02.24.06.07.53 6, 46 -- for {microscopy-at-microscopy.com} 6, 46 -- (version=TLSv1/SSLv3 cipher=OTHER); 6, 46 -- Wed, 24 Feb 2016 06:07:54 -0800 (PST) 6, 46 -- To: microscopy-at-microscopy.com 6, 46 -- From: AP Alves de Matos {apamatos-at-gmail.com} 6, 46 -- Subject: Extension of the deadline for abstract submission to Ultrapath XVIII 6, 46 -- Message-ID: {56CDB939.6020308-at-gmail.com} 6, 46 -- Date: Wed, 24 Feb 2016 14:07:53 +0000 6, 46 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 6, 46 -- Thunderbird/38.6.0 6, 46 -- MIME-Version: 1.0 6, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
An international microscopy conference. June 6 - 10 at McCrone Research Institute, Chicago
Join some of the world's leading amateur and professional microscopists for the 68th annual Inter/Micro conference, featuring: . Research presentations on techniques/instrumentation, industrial/environmental microscopy, and chemical/ forensic microscopy . Two-day workshop on microscopy of sand, taught by Thomas J. Hopen of the ATF . Evening with Brian Ford presentation: "Magical Mystery Tour of the Cell" . State Microscopical Society of Illinois Awards Dinner . ... and more! View the schedule of events.
Research presentations will be held June 6 - 8. Speakers will receive a $50 registration discount. Read abstract submission guidelines.
Use our secure online registration form at www.mcri.org to reserve your seat today.
We look forward to seeing you in Chicago!
McCrone Research Institute A Not-for-Profit Corporation 2820 South Michigan Avenue, Chicago, IL 60616-3230 Phone: 312-842-7100 Fax: 312-842-1078 www.mcri.org intermicro-at-mcri.org
......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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==============================Original Headers============================== 13, 26 -- From ph2-at-sprynet.com Wed Feb 24 09:21:30 2016 13, 26 -- Received: from elasmtp-masked.atl.sa.earthlink.net (elasmtp-masked.atl.sa.earthlink.net [209.86.89.68]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OFLUcR018529 13, 26 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 09:21:30 -0600 13, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 26 -- s=dk20050327; d=sprynet.com; 13, 26 -- b=Pt9RaMKcujcxSM58oP5jH3zXPJNwCIPl9wJnr5H2Xka2ymRIbFSVZGHyLwmaINyv; 13, 26 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 13, 26 -- Received: from [68.58.112.163] (helo=AAHHPdv7) 13, 26 -- by elasmtp-masked.atl.sa.earthlink.net with esmtpa (Exim 4.67) 13, 26 -- (envelope-from {ph2-at-sprynet.com} ) 13, 26 -- id 1aYbFR-0001Mw-68; Wed, 24 Feb 2016 10:21:05 -0500 13, 26 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 13, 26 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 13, 26 -- Subject: Call for Papers - Inter/Micro 2016 13, 26 -- Date: Wed, 24 Feb 2016 10:21:04 -0500 13, 26 -- Message-ID: {002301d16f16$fa107040$ee3150c0$-at-sprynet.com} 13, 26 -- MIME-Version: 1.0 13, 26 -- Content-Type: text/plain; 13, 26 -- charset="us-ascii" 13, 26 -- Content-Transfer-Encoding: 7bit 13, 26 -- X-Mailer: Microsoft Outlook 14.0 13, 26 -- Thread-Index: AdFvFobPmzC92okxRByiyEo5xFxtfA== 13, 26 -- Content-Language: en-us 13, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f99cb45db0fb6b68c30031faad74a9ee41350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 13, 26 -- X-Originating-IP: 68.58.112.163 ==============================End of - Headers==============================
We are getting rid of our Denton Vacuum Evaporator DV-502. If anyone out there would like a free vacuum evaporator you are welcome to come and get it. Our lab is located at UNMC in Omaha, Nebraska.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 6, 37 -- From tbargar-at-unmc.edu Wed Feb 24 11:15:11 2016 6, 37 -- Received: from zixvpm02.unmc.edu (mysecuremail.unmc.edu [192.198.54.127]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OHFBjx009521 6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 11:15:11 -0600 6, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 6, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id C5E4638520B 6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 10:55:53 -0600 (CST) 6, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 6, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 6, 37 -- (No client certificate requested) 6, 37 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id BE04B38520A 6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 10:55:52 -0600 (CST) 6, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 6, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 6, 37 -- 14.03.0235.001; Wed, 24 Feb 2016 11:14:53 -0600 6, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 6, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 37 -- Subject: Free Denton Vacuum Evaporator DV-502 6, 37 -- Thread-Topic: Free Denton Vacuum Evaporator DV-502 6, 37 -- Thread-Index: AdFvJn+TTJy4rbS3ToC6BhV9WE0Qtw== 6, 37 -- Date: Wed, 24 Feb 2016 17:14:53 +0000 6, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE854567-at-UNMCEX2.unmcresforest.org} 6, 37 -- Accept-Language: en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- x-originating-ip: [10.8.64.15] 6, 37 -- Content-Type: text/plain; charset="us-ascii" 6, 37 -- MIME-Version: 1.0 6, 37 -- X-VPM-MSG-ID: e26c73e3-4d1f-4bba-8481-375263525094 6, 37 -- X-VPM-HOST: zixvpm02.unmc.edu 6, 37 -- X-VPM-GROUP-ID: 9a1af4c2-901d-4ff8-951f-23599a9bbf45 6, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 6, 37 -- X-VPM-CERT-FLAG: 0 6, 37 -- X-VPM-IS-HYBRID: 0 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1OHFBjx009521 ==============================End of - Headers==============================
I would like to hear privately from anyone using Tescan SEM and especially anyone who is using a Tescan with the integrated Gatan ultramicrotome for serial block face imaging. I'm not familiar with this company, so all feedback would be appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Thu Feb 25 10:27:10 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1PGRAaF005835 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:27:10 -0600 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id C11455C6181 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:04:19 -0600 (CST) 7, 37 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id D36265C6173 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:04:18 -0600 (CST) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 37 -- 14.03.0235.001; Thu, 25 Feb 2016 10:26:55 -0600 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: would like to hear from Tescan customers 7, 37 -- Thread-Topic: would like to hear from Tescan customers 7, 37 -- Thread-Index: AdFv6Owu3Y0nkb+lTEWu6gBu4tHd9g== 7, 37 -- Date: Thu, 25 Feb 2016 16:26:54 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE8549F1-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: e96d80d7-3a70-4386-a1c0-04b659029cc0 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 0d5882fa-6463-4dbf-b262-a87cdbd7a309 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1PGRAaF005835 ==============================End of - Headers==============================
What are the typical software systems you use to manage on-line booking to use scopes or sample prep facilities? Either commercially available or free systems are of my interest. I would be extremely grateful if you would please share with me the experiences and expectations you have had. The system we use now allows on-line booking of a dozen resources from users, notifying users in advance, managing the cost of the usage automatically, etc. However, it's not embedded within individual equipment. The booking and the usage is not electronically linked. Ideally when a booking is made to use a scope, and this user account will implement the booking by logging in the facility on the session. Therefore the booking and the real usage will be directly linked with a true picture. Anybody is using such a system now?
Best regards, Dr Z Zhou Loughborough University, UK
==============================Original Headers============================== 4, 62 -- From Z.Zhou-at-lboro.ac.uk Fri Feb 26 08:23:05 2016 4, 62 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 4, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1QEN4xS031468 4, 62 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Feb 2016 08:23:05 -0600 4, 62 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk) 4, 62 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) 4, 62 -- (Exim 4.86) 4, 62 -- id 1aZJI2-0003KK-IE 4, 62 -- for Microscopy-at-microscopy.com; Fri, 26 Feb 2016 14:22:42 +0000 4, 62 -- Received: from EUR01-HE1-obe.outbound.protection.outlook.com (213.199.154.207) 4, 62 -- by email.lboro.ac.uk (158.125.167.4) with Microsoft SMTP Server (TLS) id 4, 62 -- 14.3.266.1; Fri, 26 Feb 2016 14:23:01 +0000 4, 62 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; 4, 62 -- s=selector1-lboro-ac-uk; 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A PostDoc position is available in the BioEM Lab of the University of Basel, Switzerland.
We are looking for a person with experience in single particle cryo-EM and image processing, using RELION, FREALIGN, EMAN2, and other packages. The position is available immediately, and funded initially for two years, with the possibility for multi-year extension and possible transition into a permanent position. The role of this person should be to perform cryo-EM sample preparation and cryo-EM data collection, and perform advanced image processing and 3D reconstructions. Expertise in single particle image analysis is required. Several high-impact biological projects are waiting, including studying larger membrane protein complexes by cryo-EM as single particles, as well as helical assemblies.
The person should serve as liaison between the BioEM Lab, which is a newly established high-resolution cryo-EM service facility at the the Center for Cellular Imaging and NanoAnalytics of the Univeristy of Basel, and the laboratory of biomolecular research at the Paul-Scherrer Institute (PSI) in Villigen. An interaction with collaborators and clients from the pharmaceutical industry interested in membrane protein structure determination is also foreseen.
This position involves high-resolution cryo-EM structural work on membrane proteins, for which our FEI Titan Krios (Quantum-LS GIF / K2 Summit) and the FEI Polara (K2 Summit) are outstanding instruments. We also operate a number of additional instruments for sample screening and cryo-EM grid analysis, including a FEI Talos, CM200FEG, T12, CM100, CM10, Versa3D, and others. Large computer clusters are available. The lab atmosphere is especially nice, and the city of Basel in Switzerland at the border to Germany and France has a beautiful historic center and a rich culture, and provides a high standard of living. This is a good place to live and to do science.
If you are interested, please contact Henning.Stahlberg-at-unibas.ch for further infos.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 11, 35 -- From henning.stahlberg-at-unibas.ch Sat Feb 27 03:26:18 2016 11, 35 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164]) 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1R9QHmM021105 11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 03:26:18 -0600 11, 35 -- Received: from localhost (localhost [127.0.0.1]) 11, 35 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id B15F23E0171 11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 10:26:02 +0100 (CET) 11, 35 -- X-Virus-Scanned: amavisd-new at unibas.ch 11, 35 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164]) 11, 35 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 11, 35 -- with LMTP id psnNyuW6_AVh for {Microscopy-at-microscopy.com} ; 11, 35 -- Sat, 27 Feb 2016 10:26:02 +0100 (CET) 11, 35 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 11, 35 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id A1F133E0185 11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 10:26:02 +0100 (CET) 11, 35 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 11, 35 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 11, 35 -- 14.03.0266.001; Sat, 27 Feb 2016 10:26:02 +0100 11, 35 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 11, 35 -- To: Microscopy {Microscopy-at-microscopy.com} 11, 35 -- Subject: PostDoc in cryo-EM and image processing in Basel, Switzerland 11, 35 -- Thread-Topic: PostDoc in cryo-EM and image processing in Basel, Switzerland 11, 35 -- Thread-Index: AQHRcUDfmNVu3pRwuU6BdfawFhkB5Q== 11, 35 -- Date: Sat, 27 Feb 2016 09:26:01 +0000 11, 35 -- Message-ID: {5C666C53-43FE-408E-A676-74146D262FF7-at-unibas.ch} 11, 35 -- Accept-Language: en-US, de-CH 11, 35 -- Content-Language: en-US 11, 35 -- X-MS-Has-Attach: 11, 35 -- X-MS-TNEF-Correlator: 11, 35 -- x-originating-ip: [131.152.226.242] 11, 35 -- Content-Type: text/plain; charset="us-ascii" 11, 35 -- Content-ID: {EBFB11FC3A4BAF469B9A3584707F0FAC-at-zuv.ads.unibas.ch} 11, 35 -- MIME-Version: 1.0 11, 35 -- Content-Transfer-Encoding: 8bit 11, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1R9QHmM021105 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both htowbin-at-amnh.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: htowbin-at-amnh.org Name: Henry Towbin
Organization: American Museum of Natural History
Title-Subject: [Filtered] Biological X-ray Analysis with SEM EDS
Message: Hi All, Can anyone provide some referenced for good biological thin film x-ray analysis? I am particularly interested in how to prepare good standards for using the Hall-Marshal or similar method to analyze C, N and P. I am trying to help some colleague with analyzing the compositions of singles celled organisms with x-ray analysis in an SEM. They are attempting to dry the cells on a formvar support and plan to measure counts from the cell and the film and then subtract the counts of the film to calculate composition. I am highly skeptical of the methods they want to use from Norland et al. 1995 (Light Element Analysis of Individual Bacteria by X-Ray Microanalysis), so any advice would be much appreciated. Thank you, Henry
Norland et al. 1995, Light Element Analysis of Individual Bacteria by X-Ray Microanalysis, http://aem.asm.org/content/61/4/1357.full.pdf ______________________________ Henry Towbin Laboratory Co-manager Microscopy and Imaging Facility American Museum of Natural History 79th St & Central Park West NY, NY 10024
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Announcing a MAS Topical Conference, Electron Probe Microanalysis 2016 May 16-19, 2016 at the University of Wisconsin-Madison
STUDENT FINANCIAL SUPPORT AVAILABLE!
TOPICAL CONFERENCE PROGRAM -Practical microanalysis for beginners to professionals -Plenary Conference including user meetings, tutorials, group discussion, problem solving, and instrument demos -Quantitative analysis using the electron microprobe and SEM with EDS and WDS -Improvements in microanalysis from measurement to accuracy -Trace element analysis -Compositional x-ray mapping and cathodoluminescence -Applications in earth science, materials science, and industry
STUDENT FINANCIAL SUPPORT AVAILABLE -Early career scholars: undergraduate, graduate, postdoc, and early career professionals may apply -Priority will be given to those with co-sponsorship from a university, advisor, or employer -ECS awardees may submit a 2-page abstract for platform or poster presentation. -Awardees will receive a maximum of $750 reimbursement for travel, lodging, and registration.
DEADLINE MARCH 15, 2016 FOR ABSTRACTS AND ECS APPLICATIONS
Please visit for more information http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016
The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:
Yaroslav Tsybovsky (Contractor) Scientist Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Post Office Box B Frederick, Maryland 21702
==============================Original Headers============================== 7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016 7, 47 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CH4NKn001027 7, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:04:23 -0500 7, 47 -- X-SBRS-Extended: Low 7, 47 -- X-IronPortListener: Outbound_SMTP 7, 47 -- IronPort-PHdr: =?us-ascii?q?9a23=3AGVHDpBbZhvUgcMpc3gRB9OH/LSx+4OfEezUN459i?= 7, 47 -- =?us-ascii?q?sYplN5qZpM66bnLW6fgltlLVR4KTs6sC0LqH9fmxEjVYvN6oizMrTt9lb1c9k8?= 7, 47 -- =?us-ascii?q?IYnggtUoauKHbQC7rUVRE8B9lIT1R//nu2YgB/Ecf6YEDO8DXptWZBUiv2OQc9?= 7, 47 -- =?us-ascii?q?HOnpAIma153xjLDjvcSCKFwQ2XKUWvBbElaflU3prM4YgI9veO4a6yDihT92Qd?= 7, 47 -- =?us-ascii?q?lQ3n5iPlmJnhzxtY+a9Z9n9DlM6bp6r5YTGZjge+wEaZAQTHF/ayFmrPHs4FPm?= 7, 47 -- =?us-ascii?q?TACV4WAXVX0Huh9JCBLC9xr9Roa3uSz//KIp/SiRJ8rtRrcsSByn7qxxTwTjjz?= 7, 47 -- =?us-ascii?q?8WcTU+9TeEpNZ3ifcRnAmwrRth2I3FJMmkPeB5ZafUY5taY0thGeB6dm0JMaiJ?= 7, 47 -- =?us-ascii?q?JcNbHuMbOv1cppe7u0AfpxygHgq9LOXuynlHgWGgjv5y6PgoDQyThF9oJNkJqn?= 7, 47 -- =?us-ascii?q?mB6YytOQ=3D=3D?= 7, 47 -- X-IronPort-Anti-Spam-Filtered: true 7, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENg?= 7, 47 -- =?us-ascii?q?XYkhXACgTY4FAEBAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTq?= 7, 47 -- =?us-ascii?q?bZIYmiQyDKYIuBZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBA?= 7, 47 -- =?us-ascii?q?Q?= 7, 47 -- X-IPAS-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENgXYkhXACgTY4FAE?= 7, 47 -- =?us-ascii?q?BAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTqbZIYmiQyDKYIuB?= 7, 47 -- =?us-ascii?q?ZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBAQ?= 7, 47 -- X-IronPort-AV: E=Sophos;i="5.24,610,1454994000"; 7, 47 -- d="scan'208";a="459727113" 7, 47 -- Received: from unknown (HELO msgb10.nih.gov) ([156.40.71.130]) 7, 47 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES256-SHA; 12 May 2016 13:04:02 -0400 7, 47 -- Received: from MSGB01.nih.gov ([169.254.1.154]) by msgb10.nih.gov 7, 47 -- ([169.254.10.4]) with mapi id 14.03.0279.002; Thu, 12 May 2016 13:04:02 -0400 7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov} 7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research, 7, 47 -- Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical 7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Index: AdGscBobBV8ZFKKkTvmemwETdQ92zg== 7, 47 -- Date: Thu, 12 May 2016 17:04:01 +0000 7, 47 -- Message-ID: {AEB1E864C83965459AE2D8A2139E1B2DD13FE7-at-msgb01.nih.gov} 7, 47 -- Accept-Language: en-US 7, 47 -- Content-Language: en-US 7, 47 -- X-MS-Has-Attach: 7, 47 -- X-MS-TNEF-Correlator: 7, 47 -- x-originating-ip: [156.40.70.6] 7, 47 -- Content-Type: text/plain; charset="us-ascii" 7, 47 -- MIME-Version: 1.0 7, 47 -- Content-Transfer-Encoding: 8bit 7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CH4NKn001027 ==============================End of - Headers==============================
The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:
Yaroslav Tsybovsky (Contractor) Scientist Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Post Office Box B Frederick, Maryland 21702
==============================Original Headers============================== 7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016 7, 47 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CH4NKn001027 7, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:04:23 -0500 7, 47 -- X-SBRS-Extended: Low 7, 47 -- X-IronPortListener: Outbound_SMTP 7, 47 -- IronPort-PHdr: =?us-ascii?q?9a23=3AGVHDpBbZhvUgcMpc3gRB9OH/LSx+4OfEezUN459i?= 7, 47 -- =?us-ascii?q?sYplN5qZpM66bnLW6fgltlLVR4KTs6sC0LqH9fmxEjVYvN6oizMrTt9lb1c9k8?= 7, 47 -- =?us-ascii?q?IYnggtUoauKHbQC7rUVRE8B9lIT1R//nu2YgB/Ecf6YEDO8DXptWZBUiv2OQc9?= 7, 47 -- =?us-ascii?q?HOnpAIma153xjLDjvcSCKFwQ2XKUWvBbElaflU3prM4YgI9veO4a6yDihT92Qd?= 7, 47 -- =?us-ascii?q?lQ3n5iPlmJnhzxtY+a9Z9n9DlM6bp6r5YTGZjge+wEaZAQTHF/ayFmrPHs4FPm?= 7, 47 -- =?us-ascii?q?TACV4WAXVX0Huh9JCBLC9xr9Roa3uSz//KIp/SiRJ8rtRrcsSByn7qxxTwTjjz?= 7, 47 -- =?us-ascii?q?8WcTU+9TeEpNZ3ifcRnAmwrRth2I3FJMmkPeB5ZafUY5taY0thGeB6dm0JMaiJ?= 7, 47 -- =?us-ascii?q?JcNbHuMbOv1cppe7u0AfpxygHgq9LOXuynlHgWGgjv5y6PgoDQyThF9oJNkJqn?= 7, 47 -- =?us-ascii?q?mB6YytOQ=3D=3D?= 7, 47 -- X-IronPort-Anti-Spam-Filtered: true 7, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENg?= 7, 47 -- =?us-ascii?q?XYkhXACgTY4FAEBAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTq?= 7, 47 -- =?us-ascii?q?bZIYmiQyDKYIuBZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBA?= 7, 47 -- =?us-ascii?q?Q?= 7, 47 -- X-IPAS-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENgXYkhXACgTY4FAE?= 7, 47 -- =?us-ascii?q?BAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTqbZIYmiQyDKYIuB?= 7, 47 -- =?us-ascii?q?ZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBAQ?= 7, 47 -- X-IronPort-AV: E=Sophos;i="5.24,610,1454994000"; 7, 47 -- d="scan'208";a="459727113" 7, 47 -- Received: from unknown (HELO msgb10.nih.gov) ([156.40.71.130]) 7, 47 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES256-SHA; 12 May 2016 13:04:02 -0400 7, 47 -- Received: from MSGB01.nih.gov ([169.254.1.154]) by msgb10.nih.gov 7, 47 -- ([169.254.10.4]) with mapi id 14.03.0279.002; Thu, 12 May 2016 13:04:02 -0400 7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov} 7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research, 7, 47 -- Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical 7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Index: AdGscBobBV8ZFKKkTvmemwETdQ92zg== 7, 47 -- Date: Thu, 12 May 2016 17:04:01 +0000 7, 47 -- Message-ID: {AEB1E864C83965459AE2D8A2139E1B2DD13FE7-at-msgb01.nih.gov} 7, 47 -- Accept-Language: en-US 7, 47 -- Content-Language: en-US 7, 47 -- X-MS-Has-Attach: 7, 47 -- X-MS-TNEF-Correlator: 7, 47 -- x-originating-ip: [156.40.70.6] 7, 47 -- Content-Type: text/plain; charset="us-ascii" 7, 47 -- MIME-Version: 1.0 7, 47 -- Content-Transfer-Encoding: 8bit 7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CH4NKn001027 ==============================End of - Headers==============================
Note: SCUR Annual meeting will take place during the first 2 days of EMC16 (the latter will take place Aug, 28 - Sept, 2 in Lyon, France)
Interested in Skin Research - Ultrastructure -Morphology - Imaging techniques and other modern techniques in Dermatological / Dermatopathological Research & Science?
Dear Colleague(s),
It is still NOT TOO LATE to submit your abstract for presentation at the forthcoming 43rd Annual meeting of SCUR- the Skin Imaging Society, 29th-30th August 2016 in Lyon, France [cf. www.scur.org]
New deadline: May 18th 2016
NOTE: 4 Travel Grants of 200[euro] for junior scientists (students and researchers under 35 y. o.) and technicians, members of SCUR (either active members or prospective new members submitting SCUR membership application simultaneously).
THREE Awards *Best young scientist presentation (200 [euro]) *Best oral presentation (200 [euro]) *Best poster presentation (200 [euro])
Please, send your abstract to { { scur.lyon2016-at-gmail.com } } (find RTF form on website www.scur.org - and link then to 'Next SCUR Meeting' and find General information at: https://www.scur.org/content/e1174/e1181/e1182 ,
at the bottom of that page also: all forms [Announcement = Invitation to Lyon - Society for Cutaneous Ultrastructure Research, {abstract submission form} , {Social Events Registration Form} , {Membership Application Form} as well as the { TRAVEL GRANT APPLICATION FORM} ]
You can visit / attend SCUR sessions if you register for whole EMC-16 - or you register only for SCUR program. (BUT: 'Registering' also for {SCUR only} must be done via the EMC-16-Website = http://www.emc2016.fr/en/register/fees - Early Bird Registering until 1 June 2016 - FIND there (if you only want to attend the SCUR-Meeting) also the rate for:
"2 Days SCUR session only (29th & 30th August) [at a special reduced rate of ] [euro] 250 " )
End your summer vacation with an exciting stop at the { Gaules capital of Lugdunum} : so many things to see and to do, apart from the scientific sessions, at the heart of the {Beaujolais region} and the { famous French kitchen} ! (visit: http://www.onlylyon.com/en/ )
SCUR 2016 ALL INFORMATION:
Thanking you for support and further distribution of this message to other interested colleagues/persons.
Looking forward to meeting YOU and many other interested Colleagues there,
With best regards,
Wolfgang MUSS (also Member of MSA) Secretary of SCUR - The Skin Imaging Society SALZBURG-AUSTRIA
==============================Original Headers============================== 27, 20 -- From wij.muss-at-aon.at Thu May 12 05:58:01 2016 27, 20 -- Received: from bsmtp2.bon.at (bsmtp2.bon.at [213.33.87.16]) 27, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CAw0SJ015148 27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 05:58:01 -0500 27, 20 -- Received: from MussTHINK (unknown [93.83.25.98]) 27, 20 -- by bsmtp2.bon.at (Postfix) with ESMTPSA id 3r592C6kH6z5tlP 27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:58:11 +0200 (CEST) 27, 20 -- From: "MUSS Wolfgang SCUR Secretary" {wij.muss-at-aon.at} 27, 20 -- To: {microscopy-at-microscopy.com} 27, 20 -- Subject: Last announcement - Abstract submission deadline extended to 18th May 2016 43rd SCUR-The SKIN IMAGING SOCIETY-Annual Meeting, LYON,29-30th August 2016 27, 20 -- Date: Thu, 12 May 2016 12:58:14 +0200 27, 20 -- Message-ID: {009101d1ac3d$2f090d80$8d1b2880$-at-aon.at} 27, 20 -- MIME-Version: 1.0 27, 20 -- Content-Type: text/plain; 27, 20 -- charset="UTF-8" 27, 20 -- X-Mailer: Microsoft Outlook 15.0 27, 20 -- Thread-Index: AdGsOXYGBS1uhIMnQHavjW1y8kY0Xw== 27, 20 -- Content-Language: de-at 27, 20 -- Content-Transfer-Encoding: 8bit 27, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CAw0SJ015148 ==============================End of - Headers==============================
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Email: ucsb.materials-at-gmail.com Name: Dawn McTague
Organization: University of California, Santa Barbara
Title-Subject: [Filtered] Job Opening at UCSB: TEM Engineer
Message: The University of California Santa Barbara Materials Department has an immediate opening for a TEM Microscopy Engineer. This position is responsible for autonomously managing the training, maintenance, operations, and research in transmission electron microscopy (TEM), atom probe microscopy, and focused ion beam (FIB) technology. This position involves high-level collaborative research with faculty, students, and research personnel in Materials and other campus units utilizing the facility. The position also provides training and instruction to students, research personnel, and outside users in advanced techniques of transmission electron microscopy in addition to maintenance and upkeep of advanced equipment. Incumbent must have a strong background in research microscopy with a high level of expertise in TEM microscopy. Experience working in a fast-paced research environment with a diverse population combined with an ability to develop thorough training programs is required. Ph.D. preferred.
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Sorry, wrong dates: August 22-27, 2016. (Lectures August 23-26).
Henning.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
On 11 May 2016, at 10:49, Henning Stahlberg {henning.stahlberg-at-unibas.ch} wrote:
Dear colleagues,
We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.
Topics include: Basics of 2D electron crystallography * Introduction to 2D electron crystallography * Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins * Cryo-EM data collection of 2D crystals * Introduction to 2DX * Drift correction using ZORRO Basic image processing with 2DX * Determination of the defocus, crystal tilt geometry, crystal lattice * Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series * 2D merging and 3D merging with reconstruction via weighted back projection * Automation of the processing pipeline Advanced topics in image processing with 2DX * Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone * Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters * Advanced usage of the 2DX backend library * Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).
Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at http://www.2dx.unibas.ch/workshop/2016
For registration, please include information about your affiliation, and a short motivation statement (max 200 words). The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,
Nikhil Biyani, Ricardo Righetto, Robert McLeod, Henning Stahlberg.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 9, 41 -- From henning.stahlberg-at-unibas.ch Wed May 11 06:30:24 2016 9, 41 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4BBUNAK005765 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 06:30:23 -0500 9, 41 -- Received: from localhost (localhost [127.0.0.1]) 9, 41 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 156422201A8 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 13:30:31 +0200 (CEST) 9, 41 -- X-Virus-Scanned: amavisd-new at unibas.ch 9, 41 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 9, 41 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 9, 41 -- with LMTP id koKeezvvHJfO for {Microscopy-at-microscopy.com} ; 9, 41 -- Wed, 11 May 2016 13:30:31 +0200 (CEST) 9, 41 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 9, 41 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 03F652201A2 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 13:30:31 +0200 (CEST) 9, 41 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 9, 41 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 9, 41 -- 14.03.0279.002; Wed, 11 May 2016 13:30:30 +0200 9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 9, 41 -- To: Microscopy {Microscopy-at-microscopy.com} 9, 41 -- CC: Ricardo Diogo Righetto {ricardo.righetto-at-unibas.ch} , 9, 41 -- Robert McLeod 9, 41 -- {robert.mcleod-at-unibas.ch} , 9, 41 -- Nikhil Biyani {nikhil.biyani-at-unibas.ch} 9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of 9, 41 -- membrane proteins with 2DX, August 22-27, 2016 9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals 9, 41 -- of membrane proteins with 2DX, August 22-27, 2016 9, 41 -- Thread-Index: AQHRq3iDqHuL+mRb9Uuzz6r21q4Q2Q== 9, 41 -- Date: Wed, 11 May 2016 11:30:25 +0000 9, 41 -- Message-ID: {AD4DFA52-B645-4E6E-A86B-39E8E9A85A36-at-unibas.ch} 9, 41 -- Accept-Language: en-US, de-CH 9, 41 -- Content-Language: en-US 9, 41 -- X-MS-Has-Attach: 9, 41 -- X-MS-TNEF-Correlator: 9, 41 -- x-originating-ip: [131.152.226.242] 9, 41 -- Content-Type: text/plain; charset="utf-8" 9, 41 -- Content-ID: {4AFC68C688518C448406D6EFD0DEB3B6-at-zuv.ads.unibas.ch} 9, 41 -- MIME-Version: 1.0 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u4BBUNAK005765 ==============================End of - Headers==============================
We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.
Topics include: Basics of 2D electron crystallography * Introduction to 2D electron crystallography * Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins * Cryo-EM data collection of 2D crystals * Introduction to 2DX * Drift correction using ZORRO Basic image processing with 2DX * Determination of the defocus, crystal tilt geometry, crystal lattice * Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series * 2D merging and 3D merging with reconstruction via weighted back projection * Automation of the processing pipeline Advanced topics in image processing with 2DX * Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone * Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters * Advanced usage of the 2DX backend library * Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).
Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at http://www.2dx.unibas.ch/workshop/2016
For registration, please include information about your affiliation, and a short motivation statement (max 200 words). The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,
Nikhil Biyani, Ricardo Righetto, Robert McLeod, Henning Stahlberg.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 9, 41 -- From henning.stahlberg-at-unibas.ch Wed May 11 03:49:28 2016 9, 41 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4B8nR35005006 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 03:49:28 -0500 9, 41 -- Received: from localhost (localhost [127.0.0.1]) 9, 41 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id 19E063E01F9 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 10:49:35 +0200 (CEST) 9, 41 -- X-Virus-Scanned: amavisd-new at unibas.ch 9, 41 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164]) 9, 41 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 9, 41 -- with LMTP id U7Dv0uAVLwUf for {Microscopy-at-microscopy.com} ; 9, 41 -- Wed, 11 May 2016 10:49:34 +0200 (CEST) 9, 41 -- Received: from URZ-HT-CAS-4.urz.unibas.ch (urz-ht-cas-4.urz.unibas.ch [131.152.8.134]) 9, 41 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id ABBFC3E0296 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 10:49:25 +0200 (CEST) 9, 41 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 9, 41 -- URZ-HT-CAS-4.urz.unibas.ch ([fe80::5431:4219:b242:4cca%12]) with mapi id 9, 41 -- 14.03.0279.002; Wed, 11 May 2016 10:49:25 +0200 9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 9, 41 -- To: Microscopy {Microscopy-at-microscopy.com} 9, 41 -- CC: Nikhil Biyani {nikhil.biyani-at-unibas.ch} , 9, 41 -- Ricardo Diogo Righetto 9, 41 -- {ricardo.righetto-at-unibas.ch} , 9, 41 -- Robert McLeod {robert.mcleod-at-unibas.ch} 9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of 9, 41 -- membrane proteins with 2DX, August 17-19. 9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals 9, 41 -- of membrane proteins with 2DX, August 17-19. 9, 41 -- Thread-Index: AQHRq2IFeqSWzMcRw0iz5Z6nMWDHlw== 9, 41 -- Date: Wed, 11 May 2016 08:49:25 +0000 9, 41 -- Message-ID: {D2C664AF-4F24-4E0D-BF2D-98895C061C72-at-unibas.ch} 9, 41 -- Accept-Language: en-US, de-CH 9, 41 -- Content-Language: en-US 9, 41 -- X-MS-Has-Attach: 9, 41 -- X-MS-TNEF-Correlator: 9, 41 -- x-originating-ip: [131.152.226.242] 9, 41 -- Content-Type: text/plain; charset="utf-8" 9, 41 -- Content-ID: {D08F1F509F7DAA49B3D4132654F1E341-at-zuv.ads.unibas.ch} 9, 41 -- MIME-Version: 1.0 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u4B8nR35005006 ==============================End of - Headers==============================
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
-----Original Message----- X-from: ajhall-at-prairienanotech.com [mailto:ajhall-at-prairienanotech.com] Sent: Tuesday, May 10, 2016 1:11 PM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
Hi Microscopy Listerve and Shouliang Zhang,
This issue has been around for a long time. In general, university equipment is there to support the research efforts of their faculty and students. Commercial analytical labs exist which can serve industrial needs. University labs, though, are often tempted to sell their services to industry to make more money. A valid argument is often raised that this is unfair competition to those commercial labs because the (very expensive) university equipment most likely has been purchased with federal grants to support academic research. Some feel this means universities should not do industrial work at all, and others feel it's OK so long as they don't undercut the commercial labs, which could open you up to a lawsuit. Some of the big commercial labs are VERY aggressive about this!
Note (for full disclosure): I am with a small commercial service lab but also work in an academic setting.
Allen J. Hall Prairie Nanotechnology www.prairienanotech.com
==============================Original Headers============================== 4, 45 -- From ajhall-at-prairienanotech.com Tue May 10 11:50:41 2016 4, 45 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) 4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AGofMY014855 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 11:50:41 -0500 4, 45 -- Received: by mail-io0-f178.google.com with SMTP id d62so23241089iof.2 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:50:47 -0700 (PDT) 4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623; 4, 45 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 45 -- :content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=t2VgeSn9eeNcFDD2LLgMyflPSDj8b8Xq67qC+mzUypN33zLwJmoTXuHN0x+SjFXYAW 4, 45 -- v4X89t2Jd3S35wojKfAdAAhJI8hiVP46ZjhbCVPLOAzTa4wYOtQGwH23UYRxx/TfALN0 4, 45 -- zTrWblxCxB+O9wlMnlGMp6pLXgVzvMwQfopuvVvHWrDPrfve984uxJ07JYv1EhFO/4XT 4, 45 -- 2MpHY5RRS1SsHmtbF4soLrMBk84IS2xbnRPUvgp/ZmXcRQfCm3w6M0bPTuXEv4S+M6eS 4, 45 -- ACAZzAtMOq2YsAmMCTcDvRyx/+OUOwC9BGA02I0on0USdiPya3uHYZcTB+goErwLxQDZ 4, 45 -- 5ySw== 4, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=1e100.net; s=20130820; 4, 45 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 45 -- :subject:content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=QFk4kWfMxbDcQtyn1C+gqxGkLC5+SjI0OA/LMHGxfEPxsP3x4V7K4CYXLufcFUVVLM 4, 45 -- c8akf4kBo8MsjTfzOGAgiFZkDsZBSJvogX2nUSazbTA7WyHvx1wqyeGothvxx0SozVg1 4, 45 -- jL3xVRlXGgGtiopZ649700HJWIg4fj2hU9+dXKRjL3ox8x9uJ3nbfOtXJ15rCrNzOfXs 4, 45 -- 1aJ7jZBrMPbIu3hq2RjvzF71eVnFf7xr/F6DJZH8TFn7bhOLCtuZ1Kkp9jVnQqGr9TMH 4, 45 -- 72aIVum6M85oHgNk9CoQGc3TkDYcqnzq+7LGykHyvwy7cTA9K/PSE/Z59z8fPwcwpTDC 4, 45 -- kCpA== 4, 45 -- X-Gm-Message-State: AOPr4FUkQov8olV3AjXGQ3Oz1CyaYwlE6ETR3r6lvbMv7pLDitpTN4n+LhJS933ONrVvDw== 4, 45 -- X-Received: by 10.107.147.138 with SMTP id v132mr41355964iod.38.1462899046675; 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Received: from AllenH.local (c-98-212-86-40.hsd1.il.comcast.net. [98.212.86.40]) 4, 45 -- by smtp.googlemail.com with ESMTPSA id uy5sm1406989igc.4.2016.05.10.09.50.45 4, 45 -- for {Microscopy-at-microscopy.com} 4, 45 -- (version=TLSv1/SSLv3 cipher=OTHER); 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Message-ID: {57321164.6080006-at-prairienanotech.com} 4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500 4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com} 4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105) 4, 45 -- MIME-Version: 1.0 4, 45 -- To: Microscopy-at-microscopy.com 4, 45 -- Subject: Re: justification of industrial user fee in a university facility 4, 45 -- Content-Type: text/plain; charset=windows-1252 4, 45 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This issue has been around for a long time. In general, university equipment is there to support the research efforts of their faculty and students. Commercial analytical labs exist which can serve industrial needs. University labs, though, are often tempted to sell their services to industry to make more money. A valid argument is often raised that this is unfair competition to those commercial labs because the (very expensive) university equipment most likely has been purchased with federal grants to support academic research. Some feel this means universities should not do industrial work at all, and others feel it s OK so long as they don t undercut the commercial labs, which could open you up to a lawsuit. Some of the big commercial labs are VERY aggressive about this!
Note (for full disclosure): I am with a small commercial service lab but also work in an academic setting.
Allen J. Hall Prairie Nanotechnology www.prairienanotech.com
==============================Original Headers============================== 4, 45 -- From ajhall-at-prairienanotech.com Tue May 10 11:50:41 2016 4, 45 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) 4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AGofMY014855 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 11:50:41 -0500 4, 45 -- Received: by mail-io0-f178.google.com with SMTP id d62so23241089iof.2 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:50:47 -0700 (PDT) 4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623; 4, 45 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 45 -- :content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=t2VgeSn9eeNcFDD2LLgMyflPSDj8b8Xq67qC+mzUypN33zLwJmoTXuHN0x+SjFXYAW 4, 45 -- v4X89t2Jd3S35wojKfAdAAhJI8hiVP46ZjhbCVPLOAzTa4wYOtQGwH23UYRxx/TfALN0 4, 45 -- zTrWblxCxB+O9wlMnlGMp6pLXgVzvMwQfopuvVvHWrDPrfve984uxJ07JYv1EhFO/4XT 4, 45 -- 2MpHY5RRS1SsHmtbF4soLrMBk84IS2xbnRPUvgp/ZmXcRQfCm3w6M0bPTuXEv4S+M6eS 4, 45 -- ACAZzAtMOq2YsAmMCTcDvRyx/+OUOwC9BGA02I0on0USdiPya3uHYZcTB+goErwLxQDZ 4, 45 -- 5ySw== 4, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=1e100.net; s=20130820; 4, 45 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 45 -- :subject:content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=QFk4kWfMxbDcQtyn1C+gqxGkLC5+SjI0OA/LMHGxfEPxsP3x4V7K4CYXLufcFUVVLM 4, 45 -- c8akf4kBo8MsjTfzOGAgiFZkDsZBSJvogX2nUSazbTA7WyHvx1wqyeGothvxx0SozVg1 4, 45 -- jL3xVRlXGgGtiopZ649700HJWIg4fj2hU9+dXKRjL3ox8x9uJ3nbfOtXJ15rCrNzOfXs 4, 45 -- 1aJ7jZBrMPbIu3hq2RjvzF71eVnFf7xr/F6DJZH8TFn7bhOLCtuZ1Kkp9jVnQqGr9TMH 4, 45 -- 72aIVum6M85oHgNk9CoQGc3TkDYcqnzq+7LGykHyvwy7cTA9K/PSE/Z59z8fPwcwpTDC 4, 45 -- kCpA== 4, 45 -- X-Gm-Message-State: AOPr4FUkQov8olV3AjXGQ3Oz1CyaYwlE6ETR3r6lvbMv7pLDitpTN4n+LhJS933ONrVvDw== 4, 45 -- X-Received: by 10.107.147.138 with SMTP id v132mr41355964iod.38.1462899046675; 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Received: from AllenH.local (c-98-212-86-40.hsd1.il.comcast.net. [98.212.86.40]) 4, 45 -- by smtp.googlemail.com with ESMTPSA id uy5sm1406989igc.4.2016.05.10.09.50.45 4, 45 -- for {Microscopy-at-microscopy.com} 4, 45 -- (version=TLSv1/SSLv3 cipher=OTHER); 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Message-ID: {57321164.6080006-at-prairienanotech.com} 4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500 4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com} 4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105) 4, 45 -- MIME-Version: 1.0 4, 45 -- To: Microscopy-at-microscopy.com 4, 45 -- Subject: Re: justification of industrial user fee in a university facility 4, 45 -- Content-Type: text/plain; charset=windows-1252 4, 45 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Many thanks for all your (so many) answers! The outcome is unambigous -- nail polish. Now up to me to test.
Kind regards, Ondrej
On 4 May 2016 1:01 pm, "Ond ej Koteck " {ondrej.kotecky-at-gmail.com} wrote:
Dear Listers,
Since years we use diamond knives to cut ultrathin sections of polymers at low temperature (around -150 *C). When cut the sections are floating boat/trough integrated on the diamond knife, from where they are collected.
To cut larger sections we started to make and use glass knives on which a plastic boat/trough is glued using a "Cavex Set Up Wax", a dentistry modelling wax, as suggested by the vendor. Unfortunately the boats fall off at low temperature.
Can anyone suggest a solution or a "glue" that would resist to low temperatures?
Many thanks, Ondrej
==============================Original Headers============================== 7, 43 -- From ondrej.kotecky-at-gmail.com Tue May 10 09:45:43 2016 7, 43 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182]) 7, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AEjhPB012444 7, 43 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:45:43 -0500 7, 43 -- Received: by mail-io0-f182.google.com with SMTP id d62so18575467iof.2 7, 43 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 07:45:48 -0700 (PDT) 7, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 43 -- d=gmail.com; s=20120113; 7, 43 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 7, 43 -- :content-transfer-encoding; 7, 43 -- bh=juGi6TFIPSER3WDp1qp0Oi/1SJybMqR1uPUPBQ95RpA=; 7, 43 -- b=NuhwnPcDJx8SuCFQ79A6qP98FEo1b0fr20ciZtWKnmot3xUS8KR1gjGsAl8KjxH2hS 7, 43 -- FNTOyULlsjNktoBCL9oWgOBcpz69WYXolkKC8TRwLBlE3fzl5aPJCQLB7rrWPvDY4uj5 7, 43 -- dzZCpKLFK79fYObe8lrEz3ZESrHxe6TL7jC2RACL3eRwanw+e4y+6e6W4LsiA+1cVrc0 7, 43 -- zAV25AifBQpMvVFGnalzzBZ1RGtVs9cNrRWMB3A6qnwzVUbz6C/NwchNFNfzTZTJInpP 7, 43 -- X4LRWO7c1+2Pw5wDP1it8hvrLs1dT4dlNP7SuIKx8uoV82U/4GLUjyUv/4XZo/rIQmoO 7, 43 -- q7Aw== 7, 43 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 43 -- d=1e100.net; s=20130820; 7, 43 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date 7, 43 -- :message-id:subject:to:content-transfer-encoding; 7, 43 -- bh=juGi6TFIPSER3WDp1qp0Oi/1SJybMqR1uPUPBQ95RpA=; 7, 43 -- b=PWISH5GIMOOqvOEBksuPZ5ybnXbsykEShkZDlgusIIBzPUj7GuEzEeHDWkecPafEGe 7, 43 -- ef1rSSjg+VP2APlUAHQ478F3X+1IeUlO1wEG04ywYDdEm795GlfSmlcrE59zAPEo6x2D 7, 43 -- Ud4Dq3zyfdwfC+zI/g625g5dPSTjCVPyE+xIuqxgsdMM82Cw1IflW+OwJb3BOL3tjRzW 7, 43 -- hJgVF/aZ/Gk56h8Jm4oroZ6Ez5FcRJ7wj2VHNELHRnKdhbyImrmF57Qr1GkSVC4VV2Ng 7, 43 -- JS4YYEtlO6OLPR89mZzQc1fKyEsBCoTsKGJ48cRGpUbqdeIV6b30kLUG2+IZ9JwCExMs 7, 43 -- G02w== 7, 43 -- X-Gm-Message-State: AOPr4FWdqDkr2V2jSjFsXg13Sw4hc/iAyHYTiXtu5CglslkrMZmeOuZPruRxIRKG0fpLPdyu0Qh9v/g1mQ2cZg== 7, 43 -- X-Received: by 10.107.163.75 with SMTP id m72mr40810450ioe.79.1462891548277; 7, 43 -- Tue, 10 May 2016 07:45:48 -0700 (PDT) 7, 43 -- MIME-Version: 1.0 7, 43 -- Received: by 10.107.26.68 with HTTP; Tue, 10 May 2016 07:45:29 -0700 (PDT) 7, 43 -- In-Reply-To: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com} 7, 43 -- References: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com} 7, 43 -- From: =?UTF-8?B?T25kxZllaiBLb3RlY2vDvQ==?= {ondrej.kotecky-at-gmail.com} 7, 43 -- Date: Tue, 10 May 2016 16:45:29 +0200 7, 43 -- Message-ID: {CAJRapMB8sj1Yu1_QHzaAUvBe4xNMujrAudqEim=S6hppyV1_RA-at-mail.gmail.com} 7, 43 -- Subject: Re: Cryomicrotomy using glass knives 7, 43 -- To: microscopy-at-microscopy.com 7, 43 -- Content-Type: text/plain; charset=UTF-8 7, 43 -- Content-Transfer-Encoding: 8bit 7, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4AEjhPB012444 ==============================End of - Headers==============================
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Email: mjbehr-at-dow.com Name: Michael Behr
Organization: The Dow Chemical Company
Title-Subject: [Filtered] JEOL 2010F
Message: Free JEOL 2010F in working condition, with Gatan Enfina, and Bruker XEDS. You pay for removal from facility in Midland, MI, and shipping to your location. Available June 2016.
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Email: fei.long-at-queensu.ca Name: Fei Long
Organization: Queen's University
Title-Subject: [Filtered] Used 3mm TEM disc puncher, and Tenupol-5 jet
Message: Hi all, I am looking to buy a used TEM 3mm disc puncher. Also a set of the jets with 1mm bore for Tenupol-5 electro-polisher, since the ones I have is broken now.
If anyone has these spare or none used parts, I am interested to purchase them. Thanks,
Fei
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Colleagues, For any film aficianados out there, I have a cameraback and mounting bits, as well as the electronic controller, for a 35 mm camera, that coupled to a Zeiss Axioplan. I was using it up until around 2002 or so, when it was working fine. It has been kept cool and out of dust since then. I think it could probably be adapted to any brand of scope with a bit of hardware tinkering. Free to anyone who wants it (I am willing to pack it carefully for shipment but not pay shipping costs). Thanks, Tobias University of Massachusetts, Biology Department, 611 N Pleasant St, Amherst, MA, 01003, USA
==============================Original Headers============================== 1, 21 -- From baskin-at-bio.umass.edu Mon May 9 16:11:00 2016 1, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49LB0q8023456 1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 16:11:00 -0500 1, 21 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 1, 21 -- (authenticated bits=0) 1, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u49LB2gc013828 1, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO) 1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 17:11:02 -0400 1, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 1, 21 -- Subject: 35 mm camera and controller for Zeiss Axioplan to give away 1, 21 -- To: microscnet {microscopy-at-microscopy.com} 1, 21 -- Message-ID: {0ff1830d-6227-1e06-a78e-6a1fbc205134-at-bio.umass.edu} 1, 21 -- Date: Mon, 9 May 2016 17:11:02 -0400 1, 21 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 1, 21 -- Gecko/20100101 Thunderbird/45.0 1, 21 -- MIME-Version: 1.0 1, 21 -- Content-Type: text/plain; charset=utf-8; format=flowed 1, 21 -- Content-Transfer-Encoding: 7bit 1, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Mon, 09 May 2016 17:11:02 -0400 (EDT) 1, 21 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
I agree with Michael's comments, these holes are typical for uninfiltrated dense material. Try letting your specimens sit in uncatalyzed resin at room temperature a bit longer. For Epon I usually do 3h 25%, over night 50% and 2x2h 100% before going into catalyzed resin at 60C 24h. Leaving out the catalyst will also improve the viscosity. You can even heat up the uncatalyzed resin to 37C if you want to push it. You do have quite some crystal damage from freezing, that together with a dry acetone FS will extract a considerable amount of material (see my follow up paper to Walther & Ziegler for more details, http://www.ncbi.nlm.nih.gov/pubmed/18445157 ). Poorly frozen material extracts a lot easier. Here are a few suggestions:
1. If you have adherent cells on sapphire discs, lift them out of the culture dish, touch a filter paper to remove excess medium, dip in hexedecene, then mount for HPF. This ensures you're only freezing the minimal layer of medium with your cells. Freezing adherent cells in pure medium and 200 um deep carriers wil almost certainly fail without cryoprotection as you're essentially tryin to freeze 200 um of pure water. (safety tip: repeated skin exposure to hexadecene will give you contact dermatitis, wear nitrile gloves). 2. If you're working with suspensions, 100 um deep carriers and 20% BSA for cryoprotection works well with most cells and doesn't mess up your FS (in contrast to sugars that won't come out easily during FS). 3. FS in acetone, about 1% osmium tetroxide, about 0.1% uranyl acetate, about 5% water for best contrast. To get there, dissolve OsO4 in acetone, add 1:20 of your 2% aqueous UA and that's it. Some precipitation is normal and doesn't influence the end result, it's a saturated solution particularly at cold temperatures. If it's completely snowed up, try half the amount of 2% UA. 4. I'd advise against prefixation in aldehydes unless your cells are very sensitive (e.g. primary cells) and you can't get them to the HPF within 30ish minutes.
Good luck! Chris
-----Original Message----- X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] Sent: Monday, May 9, 2016 10:24 AM To: cbuser125-at-gmail.com
Hello Elizabeth and Welcome to the Forum, These "holes" look like un-infiltrated granules. I would see this sometimes with Drosophila eyes and the pigment granules. Some would be infiltrated and others not. The fix was to do longer and more infiltration steps (resin:solvent) and/or switch to a less viscous resin. Were these cells grown on a sapphire disc or is this a suspension that was HPF/AFS? I know mast cells have a large number of secretory granules, but not outside the cells plasma memebrane. Also what is you AFS cocktail? I would also introduce 5% water to your freezing cocktail for better membrane preservation, (Walther, P. & Ziegler, A. Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3-10). Also what was your cryo-protectant? (what did you HPF the cells in). I believe the HPF images should look a lot better, it looks like there is some freezing artifacts in your cells (look for spider-web like profiles) and your mitochondria are extracted. Were these cells frozen live? If they are clinical you can use the HPF-chemical hybrid technique where you lightly fix the sample in paraformaldehyde only until you can get to the HPF. If you need a good reference for the HPF/AFS techniques, Mary Morphew at Colorado (formerly Kent McDonald's lab) has a very detailed manual online: https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF experiments should be accompanied by the standard fixation counterpart for comparison. Perhaps this will give you a clue. Please keep us posted as to you results.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, May 09, 2016 9:05 AM To: mdelann1-at-jhmi.edu
X-from: elisabeth.pritz-at-medunigraz.at
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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Hi I have very limited experience in TEM so please don't laugh: could they be air associated or originated from the cell, that formed bubbles during the procedure? yorgos
-----Original Message----- X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] Sent: Monday, May 9, 2016 8:12 PM To: eikonika-at-otenet.gr
Hello Elizabeth and Welcome to the Forum, These "holes" look like un-infiltrated granules. I would see this sometimes with Drosophila eyes and the pigment granules. Some would be infiltrated and others not. The fix was to do longer and more infiltration steps (resin:solvent) and/or switch to a less viscous resin. Were these cells grown on a sapphire disc or is this a suspension that was HPF/AFS? I know mast cells have a large number of secretory granules, but not outside the cells plasma memebrane. Also what is you AFS cocktail? I would also introduce 5% water to your freezing cocktail for better membrane preservation, (Walther, P. & Ziegler, A. Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3-10). Also what was your cryo-protectant? (what did you HPF the cells in). I believe the HPF images should look a lot better, it looks like there is some freezing artifacts in your cells (look for spider-web like profiles) and your mitochondria are extracted. Were these cells frozen live? If they are clinical you can use the HPF-chemical hybrid technique where you lightly fix the sample in paraformaldehyde only until you can get to the HPF. If you need a good reference for the HPF/AFS techniques, Mary Morphew at Colorado (formerly Kent McDonald's lab) has a very detailed manual online: https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF experiments should be accompanied by the standard fixation counterpart for comparison. Perhaps this will give you a clue. Please keep us posted as to you results.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, May 09, 2016 9:05 AM To: mdelann1-at-jhmi.edu
X-from: elisabeth.pritz-at-medunigraz.at
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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Hello Elizabeth and Welcome to the Forum, These "holes" look like un-infiltrated granules. I would see this sometimes with Drosophila eyes and the pigment granules. Some would be infiltrated and others not. The fix was to do longer and more infiltration steps (resin:solvent) and/or switch to a less viscous resin. Were these cells grown on a sapphire disc or is this a suspension that was HPF/AFS? I know mast cells have a large number of secretory granules, but not outside the cells plasma memebrane. Also what is you AFS cocktail? I would also introduce 5% water to your freezing cocktail for better membrane preservation, (Walther, P. & Ziegler, A. Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3-10). Also what was your cryo-protectant? (what did you HPF the cells in). I believe the HPF images should look a lot better, it looks like there is some freezing artifacts in your cells (look for spider-web like profiles) and your mitochondria are extracted. Were these cells frozen live? If they are clinical you can use the HPF-chemical hybrid technique where you lightly fix the sample in paraformaldehyde only until you can get to the HPF. If you need a good reference for the HPF/AFS techniques, Mary Morphew at Colorado (formerly Kent McDonald's lab) has a very detailed manual online: https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF experiments should be accompanied by the standard fixation counterpart for comparison. Perhaps this will give you a clue. Please keep us posted as to you results.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, May 09, 2016 9:05 AM To: mdelann1-at-jhmi.edu
X-from: elisabeth.pritz-at-medunigraz.at
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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To recap: I placed a request for opinions on photo printers for micrographs, now that HP seems to be out of the game. I got a few responses (surprisingly few, given past interest in this topic), but those that expressed a preference all liked the Canon P600. Which does look good on paper (it's Monday morning, so that's the best pun I've got).
Thanks to all who responded.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 30 -- From oshel1pe-at-cmich.edu Mon May 9 08:32:00 2016 3, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49DW0KG025044 3, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 08:32:00 -0500 3, 30 -- Received: from cas.cmich.edu ([141.209.15.90]) 3, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u49DVxZv025935 3, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 09:32:03 -0400 3, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 3, 30 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 3, 30 -- (TLS) id 14.3.248.2; Mon, 9 May 2016 09:32:00 -0400 3, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 3, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Mon, 9 May 3, 30 -- 2016 09:32:00 -0400 3, 30 -- Message-ID: {57309150.4030202-at-cmich.edu} 3, 30 -- Date: Mon, 9 May 2016 09:32:00 -0400 3, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 30 -- MIME-Version: 1.0 3, 30 -- To: micro {microscopy-at-microscopy.com} 3, 30 -- Subject: New photo printer for micrographs, for those interested 3, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 30 -- Content-Transfer-Encoding: 7bit 3, 30 -- X-Originating-IP: [141.209.2.100] 3, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 30 -- X-Spam-Score: -0.30 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 30 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 30 -- X-CanItPRO-Stream: default 3, 30 -- X-Canit-Stats-ID: 02QQdw3IV - 77565d9be353 - 20160509 3, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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Email: scottserata-at-gmail.com Name: Scott Serata
Organization: California Academy of Sciences/JEOL
Title-Subject: [Filtered] detailed instructions for LEO (Zeiss) 1450VP SEM
Message: Several people have asked me to post this on the listserve. I have written detailed instructions for the LEO/Zeiss 1450VP SEM. I was the engineer at the California Academy of Sciences responsible for training users and fixing this machine for 15 years, until I finally got rid of it last year. (I now work for JEOL) Our machine did not have any analytical equipment (X-ray EDS/WDS) on it, just the SE, VPSE and BSD detectors. If you have a LEO 1450 VP SEM this maybe useful. It is in PDF format and about 13 MB.
Scott Serata
click on this link to get it from my Google drive https://drive.google.com/open?id=0B2-Pe5FzqYv2TWdUNXNLQTZLQmM
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Title-Subject: [Filtered] Free Megaview II CCD System for TEM
Message: TSS has one complete Megaview II CCD Side-mount Camera for Philips/FEI Series TEM s that needs to find a home. Functional and complete side-mount CCD camera solution with computer and cable set. Pictures may be seen of the camera/flange here:
https://tss.exavault.com/share/view/bemn-fgdqg1k7
Will carefully prepare for shipment and with your shipping label and send to you anywhere in the world.
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Like you say, we take a look at local rates for similar services and price = higher so as to not undercut the local business.
If anyone gives me a hard time about rates I compare it to what they would = pay a professional photographer to take high school senior pictures or wedd= ing photos. EM Lab rates start to look entirely reasonable, especially give= n our "camera" is on the order of hundreds of thousands of dollars instead = of hundreds of dollars.
Kind regards,
William Stonewall Monroe University of Alabama -at- Birmingham SEM Lab http://www.uab.edu/scanningelectronmicroscopy
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Title-Subject: [Filtered] justification of industrial user fee in a uiversity facility Message: Dear listers, How do your facilities, especially for the electron microscopes, justify the industrial user rate? Do you consider the local analytical companies rate so that yours won't undercut theirs? I looked up the SEM/TEM rate across the universties nationwide and found it varies a lot, ranging from less $100/h to $300/h for industrial users. Thanks.
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==============================Original Headers============================== 10, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 20:16:25 2016 10, 52 -- Received: from mail-qg0-f44.google.com (mail-qg0-f44.google.com [209.85.192.44]) 10, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u461GPbK024302 10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 20:16:25 -0500 10, 52 -- Received: by mail-qg0-f44.google.com with SMTP id f92so49409392qgf.0 10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 18:19:18 -0700 (PDT) 10, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 52 -- d=gmail.com; s=20120113; 10, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 10, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 10, 52 -- bh=SDeVEu/IJUmDBKJGbWPt3Vw9+AGmU17Cu4hF+DptxCE=; 10, 52 -- b=fQ8BJ3dKsgXkISNj1F7oymt9I/vYRclrSDoCezWviCZ6tk/UDlURrZEGyvMtvqibX8 10, 52 -- QMAL+Hor5mIbbwwnYyNEGFWl35xXCUO6tFujxoZm2ixEUpDR1ekxnyBrVYK+tAJYKdWi 10, 52 -- oVsnu1yHtpXCoY7P8GWlV9wCDpm32h0zyAYMDti6vh3Cd8/InnxH+haX1ZiLZr0OPH9d 10, 52 -- Z70xUjUGk7TaXuQUWvXEUidTGIUkoZirinx0kbRZnhKozBQ7GxEgaKZatm68H+s6iXTt 10, 52 -- 2RrcoKOmvormf1RQ4Lza3wKH5APsH+eZ0XMgmQH9fL8AEckZkGlua2RDY6oxZy0pFSC2 10, 52 -- mrng== 10, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 52 -- d=1e100.net; s=20130820; 10, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 10, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 10, 52 -- bh=SDeVEu/IJUmDBKJGbWPt3Vw9+AGmU17Cu4hF+DptxCE=; 10, 52 -- b=BLJLq3IuTYAtaqeYF6A2mLk81ZByE6eFW4Qe3LjPqPu5VqWL53LHy2LCtuJwhQY1Ja 10, 52 -- VvC5WUdG4eysRt5VJSIW+EkoBvpD3lrtn9hd09TPdN1FshILQyAFsuoaDTWr0A+a/CNO 10, 52 -- 5FG0mGIjaliyBr/8cHhVBjOQJlSyEYlN8jAJCGvbSykdYawcy+Yrtn4LDuYofenkU6b9 10, 52 -- GiatPekKT9AwWkBAc57eennrQRkh5oJDwgyW+k9OLs6HQTkZ/jFFmh3Px7WHP6PhMwZe 10, 52 -- lOoR1N3FIXG8AXUBs0V0nOpEqxuERu+p3QU6XKggQZjziqTa4GHKpJ362C5CqjTc6/fd 10, 52 -- QE2g== 10, 52 -- X-Gm-Message-State: AOPr4FUnms36E2y6Op368tFk6PCdzGcWmv7QzVQlHlvu5dwyafpGmlUn5tb+3V+M5xb+zQ== 10, 52 -- X-Received: by 10.140.22.203 with SMTP id 69mr2840140qgn.62.1462497557768; 10, 52 -- Thu, 05 May 2016 18:19:17 -0700 (PDT) 10, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) 10, 52 -- by smtp.googlemail.com with ESMTPSA id c33sm4694733qga.39.2016.05.05.18.19.16 10, 52 -- for {microscopy-at-microscopy.com} 10, 52 -- (version=TLSv1/SSLv3 cipher=OTHER); 10, 52 -- Thu, 05 May 2016 18:19:17 -0700 (PDT) 10, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 10, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 10, 52 -- {microscopylistserver-noreply-at-microscopy.com} 10, 52 -- Subject: viaWWW:justification of industrial user fee in a uiversity facility 10, 52 -- References: {201605052145.u45Ljrio019548-at-ns.microscopy.com} 10, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 52 -- X-Forwarded-Message-Id: {201605052145.u45Ljrio019548-at-ns.microscopy.com} 10, 52 -- Message-ID: {4bb26c2f-1c69-d2f5-6069-af4d0aefb8dd-at-microscopy.com} 10, 52 -- Date: Thu, 5 May 2016 20:19:16 -0500 10, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 10, 52 -- Gecko/20100101 Thunderbird/45.0 10, 52 -- MIME-Version: 1.0 10, 52 -- In-Reply-To: {201605052145.u45Ljrio019548-at-ns.microscopy.com} 10, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If you are not fussy or fancy just drive some sharpened 1/8" diameter stainless steel rods through a clean rubber stopper. Will work OK through the HV range (10^-6 Torr) , but probably not into the UHV range.
==============================Original Headers============================== 1, 42 -- From bigelow-at-umich.edu Thu May 5 13:47:37 2016 1, 42 -- Received: from mail-wm0-f46.google.com (mail-wm0-f46.google.com [74.125.82.46]) 1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45Ilbls023188 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 13:47:37 -0500 1, 42 -- Received: by mail-wm0-f46.google.com with SMTP id a17so42508274wme.0 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 11:50:28 -0700 (PDT) 1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=umich.edu; s=google; 1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 1, 42 -- :content-transfer-encoding; 1, 42 -- bh=3bbbWfX1sDpnKVTCO89Hjj6HEN6J/n3Vy9oX4WnYMco=; 1, 42 -- b=PN2s3z03LUl1lZCMHVkAk6dMHP+JXoAvKck86xJqHqeaeCYek/hpDUFtu4flG8dyoh 1, 42 -- BwRgQkEtLZPAF9TTyNwVxbjI15hWNxMuE8/YnFQ7McADcY34/OsFLzYcLT3StJTlUE6U 1, 42 -- V6k4NJoCHQ1wxN87xkNFHw9FHJcyI3Lju0TJc= 1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=1e100.net; s=20130820; 1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 1, 42 -- :subject:content-transfer-encoding; 1, 42 -- bh=3bbbWfX1sDpnKVTCO89Hjj6HEN6J/n3Vy9oX4WnYMco=; 1, 42 -- b=JqeRpKe/fqzx8vZr+8gHGkt49U/a/Eo/oFeKCujXMVUUI+A97c5wTFBGt8/1ib4Bkt 1, 42 -- wlIYewSTHCTzivwrzGhSbwcqO2P52qREVVx1rqEzE4ytHh3UKE/RKQqoFa1HlOqo/ofJ 1, 42 -- zLllgfb3z38RieqYFUPVTInWguY9gjusF2NIxNxP6ADgkJ3Frr4fKSCNiOWxjdznnRgM 1, 42 -- x41jLxNcbfQFq0F6TBQg4KTS6d96Czotn7Lu9Llw6VwWXqHIF4b+JCYPrSGteiMtUCKx 1, 42 -- jbFCBjICyucgqPeAgm9sbTCIBJ69Q7t8WXBQJE75A97toiqercgV4qlr5Lqqlz1idhyb 1, 42 -- pB4w== 1, 42 -- X-Gm-Message-State: AOPr4FUd9PlZ9IePDj8akeu3j5TZchle2s1JpKnDShGEsCOLRjQ503pse6ojUlPCeoI6I0JL 1, 42 -- X-Received: by 10.194.139.104 with SMTP id qx8mr15321433wjb.14.1462474228130; 1, 42 -- Thu, 05 May 2016 11:50:28 -0700 (PDT) 1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 1, 42 -- by smtp.gmail.com with ESMTPSA id i194sm4492750wmf.6.2016.05.05.11.50.27 1, 42 -- for {microscopy-at-microscopy.com} 1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 1, 42 -- Thu, 05 May 2016 11:50:27 -0700 (PDT) 1, 42 -- Message-ID: {572B95F1.1060904-at-umich.edu} 1, 42 -- Date: Thu, 05 May 2016 14:50:25 -0400 1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 42 -- MIME-Version: 1.0 1, 42 -- To: microscopy-at-microscopy.com 1, 42 -- Subject: [Microscopy]Electrical feedthroughs 1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
My friend recently made a feedthrough using stiff piano wire, a rubber stopper from home depot, and super glue (I think, or maybe it was torr-seal type epoxy). He used a DB9 port to mark the pin locations, then drilled pilot holes (which I don't think were fully drilled through the rubber) and I believe then he pounded the wire through the remainder of the rubber. This stopper was jammed into a tapered hole in a blank/unused blocking plate that came with his machine. It holds working pressure as good as before the feedthrough was installed. If you want more info, I can probably pass you his direct email to ask for more details.
On Thu, May 5, 2016 at 5:39 AM, {microscopy.listserver-at-gmail.com} wrote:
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: CHANL UNC
Title-Subject: [Filtered] electrical pass through for FEI ESEM Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports. thanks Wallace Login Host: 152.19.204.249 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Microscopy] RE: Cryomicrotomy using glass knives
Message: Hi Ondrej,
At Delta microscopy school some of us used 3M Silver Polyester Tape, Nonconductive, 850 from Ted Pella to make boats for glass knives. I think the tape is rated for -150 degrees C. Ted Pella also carries other type tapes that may be used for making boats with lower temp ranges. You might check.
Regards,
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Email: peter.eschbach-at-oregonstate.edu Name: Peter Eschbach
Organization: Oregon State University
Title-Subject: [Filtered] Importing .txt files into Digital Micrograph for EELS
Message: Hi:
We do not have a digiscan box to control our Titan from Digital Micrograph (DM). So, for now we are collecting simultaneous EELS and EDX linescans in FEI's TIA software. I can do rudimentary analysis in TIA on all the EELS data but would like to import several EELS spectra from my linescan into DM. I am able to save the TIA acquired EELS data into .txt files with two nice columns, Energy and Intensity. But, does anybody know of a script to convert the .txt files into DM3? We looked on David Mitchell's site, but nothing jumped out. Thanks, Pete Eschbach Oregon State University EM Facility 541 737 5645
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: CHANL UNC
Title-Subject: [Filtered] electrical pass through for FEI ESEM Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports. thanks Wallace Login Host: 152.19.204.249 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Don: A pumping speed of 1 cfm is a pretty high rate of flow (28 liters/min) and typical of values for small rotary vane pumps. For this type of pump the speed is usually rated as the volume of gas moved through the pump when both the inlet and outlet ports are at atmospheric presure. I wonder if you would really need a pumping speed of 1 cfm for a device that merely holds a wafer in place. The question is, what is the actual volume that needs to be evacuated, and how much of a hurry are you in to get the wafer held firmly in place. If you just need to evacuate a tube of modest diameter and modest length leading to a small chamber under the wafer you certainly don't need a speed of 1 cfm to do the job, even if the system leaks pretty badly.
Wil
==============================Original Headers============================== 2, 42 -- From bigelow-at-umich.edu Wed May 4 19:24:32 2016 2, 42 -- Received: from mail-wm0-f44.google.com (mail-wm0-f44.google.com [74.125.82.44]) 2, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u450OVBO002754 2, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 19:24:32 -0500 2, 42 -- Received: by mail-wm0-f44.google.com with SMTP id a17so2635129wme.0 2, 42 -- for {microscopy-at-microscopy.com} ; Wed, 04 May 2016 17:27:20 -0700 (PDT) 2, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 42 -- d=umich.edu; s=google; 2, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 2, 42 -- :content-transfer-encoding; 2, 42 -- bh=hq58s9xkmj+ZbM9fQ2wwIYVZX1qv8u2pIM2DIldDGuc=; 2, 42 -- b=IcuIgLDQ5CQ/kyLNfMDtVoNXfzUT37OybdkPqO2V5iYhAYD7FYCiYV7v68vKxClQ9H 2, 42 -- OQ3G61zMM9K6Z+IwnIDM0/5rHL0vM9Yp9Nd8n8Ht5VWfAN99YrsgMKeZ4eLHpBk5OBHr 2, 42 -- V2KYYK9NiN9VK6AeIhTbKYk/WxED89i2fHy8I= 2, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 42 -- d=1e100.net; s=20130820; 2, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 2, 42 -- :subject:content-transfer-encoding; 2, 42 -- bh=hq58s9xkmj+ZbM9fQ2wwIYVZX1qv8u2pIM2DIldDGuc=; 2, 42 -- b=NKBQMOmf+dccqKPbu/tmIUz3H/ZLU+qKGpmzDtVD43h9LQfCiHKC2a1NzN0z2zU2T9 2, 42 -- LejstTbCX51qWliRLGYVsXyyKRIVvfmQrHwEOn97pDfzd67e+iEZJS9o8j3j2wiQkHh/ 2, 42 -- +Lw4WSeQAb3XrkZzvngaGjABibh9LBUbMT92dXzw1IwC5Qog3hK7A/sEPyAUGuwgu3g5 2, 42 -- U3L2Uj7MlUuB+/pFB1TEUDCbh5Ka3QW7qYSwLpseMdUmQ2nTysDjQW6VYMPH283V81IZ 2, 42 -- /knMFuE6SqYNyuFTS9AcV3Ej2tEhcQoIz3Ja1cLjGHQcs6zzV8SjJh5fkt85iWzYtgtD 2, 42 -- b3Ng== 2, 42 -- X-Gm-Message-State: AOPr4FU8hFs4U2inG+8+tUz9dN4gs81y+zjx7L9vV6Jdxkl82deva3Eo3VjgTH5dm+69d1KA 2, 42 -- X-Received: by 10.194.187.236 with SMTP id fv12mr11238503wjc.96.1462408039588; 2, 42 -- Wed, 04 May 2016 17:27:19 -0700 (PDT) 2, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 2, 42 -- by smtp.gmail.com with ESMTPSA id on2sm6872092wjc.32.2016.05.04.17.27.18 2, 42 -- for {microscopy-at-microscopy.com} 2, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 2, 42 -- Wed, 04 May 2016 17:27:19 -0700 (PDT) 2, 42 -- Message-ID: {572A9365.600-at-umich.edu} 2, 42 -- Date: Wed, 04 May 2016 20:27:17 -0400 2, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 2, 42 -- MIME-Version: 1.0 2, 42 -- To: microscopy-at-microscopy.com 2, 42 -- Subject: [Microscopy]Pump speed for wafer holder 2, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Fred, Thanks very much for this explanation. Don
----- Original Message ----- From: Frederick Schamber To: Don Chernoff at ASM Sent: Wednesday, May 04, 2016 5:31 PM Subject: [a] Re: how are vacuum pumps rated?
Three things: (1) Vacuum pump ratings (pumping speed) always apply to the inlet (evacuated).
(2) If they say cfm (or lps) it is 'pumping speed' and that is independent of either internal or external pressure. Think of having a 'demon' collecting one cubic foot of gas in a (previously evacuated) canister while inside the chamber. The demon then seals the canister and removes it from the chamber. To repeat the cycle he shrinks the canister to zero volume ( thereby expelling the collected gas) and then goes back into the chamber where he expands it to 1 cubic foot again. If he does this once a minute, that's a 1 cfm pumping speed. It's easiest to see that's what a piston pump does but it's the same principle for rotary vane (and can be generalized for a turbo). If you think of the 'demon' model it's clear that the volume per second is a function only of the canister size and repetition rate, not the pressure (either internal nor external. That's an idealization for an actual pump of course due to things like leakage, turbulent flow, and lots of other messy things. But in principle, volume pumping speed is constant.
(3) Mass flow rate is density times pumping speed and would be something like grams per second. Since the density of the gas decreases, the mass flow rate also decreases with improving vacuum. A pump's 'throughout' is defined as inlet pressure x pumping speed and is proportional to mass flow rate.
Actually, mass flow rate (and throughout) can be defined and is invariant anywhere in the system, so long as volume and density are defined at the same place. So you can use the constancy of throughput to calculate the volume of gas exhausted for a given pumping speed and inlet pressure.
Any help?
Fred
Sent from my iPhone
On May 4, 2016, at 4:14 PM, Don Chernoff at ASM {donc-at-asmicro.com} wrote:
Dear Fred, Thanks for your reply. I you're pointing in the right direction. My question is a practical one: if someone says "you need a flow rate of 1 cfm" does that mean 1 cfm measured at P = 1 atmosphere? Don ----- Original Message ----- From: Frederick Schamber To: donc-at-asmicro.com Sent: Wednesday, May 04, 2016 3:13 PM
Dear Fred, Thanks for your reply. I you're pointing in the right direction. My question is a practical one: if someone says "you need a flow rate of 1 cfm" does that mean 1 cfm measured at P = 1 atmosphere? Don ----- Original Message ----- From: Frederick Schamber To: donc-at-asmicro.com Sent: Wednesday, May 04, 2016 3:13 PM Subject: [a] Re: [Microscopy] how are vacuum pumps rated?
Don,
Maybe I misunderstand your question, but I was long puzzled by how mechanical vacuum pumps are rated. Finally I realized that the mechanism of a vacuum pump will capture and remove a constant volume of gas regardless of internal pressure. That is, regardless of that internal pressure, the volume swept out remains the same. Clearly, that means that the volume of gas exhausted decreases proportionately as the internal pressure declines. This is why we use 'mass flow' when we want to quantify the mass of material pumped.
So if you are looking at volume of gas removed from the chamber the pumping speed is (essentially) constant. But if you are looking at volume or mass of gas exhausted, the pumping speed varies proportionately to internal pressure.
Does this help?
Fred Schamber (retired, but still interested)
On Wed, May 4, 2016 at 2:27 PM, {donc-at-asmicro.com} wrote:
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Dear All, I want to thank the many people who responded to my initial post about low vacuum pump.
In reviewing vacuum pump specifications, I note that the flow rate chart shows a high rate at atmospheric pressure with a rapid decrease in rate as the input pressure decreases. When this type of pump is specified, is there general agreement that the flow rate is specified for free air (at 1 atmosphere pressure)? regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: Don Chernoff at ASM To: Microscopy List Sent: Sunday, May 01, 2016 7:01 PM Subject: low vacuum pump
I'm working with a wafer inspection system that uses vacuum to clamp the wafer. The manufacturer's specifications call for a modest vacuum:
low vacuum: 150 torr (-24" Hg)
flow rate: { 1 cfm (28 l/m)
Actual flow will be intermittent.
What type of pump would be suitable for this application?
I welcome suggestions for specific models and suggestions for how to configure the pump.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
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Dear All, I want to thank the many people who responded to my initial post about low vacuum pump.
In reviewing vacuum pump specifications, I note that the flow rate chart shows a high rate at atmospheric pressure with a rapid decrease in rate as the input pressure decreases. When this type of pump is specified, is there general agreement that the flow rate is specified for free air (at 1 atmosphere pressure)? regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: Don Chernoff at ASM To: Microscopy List Sent: Sunday, May 01, 2016 7:01 PM Subject: low vacuum pump
I'm working with a wafer inspection system that uses vacuum to clamp the wafer. The manufacturer's specifications call for a modest vacuum:
low vacuum: 150 torr (-24" Hg)
flow rate: { 1 cfm (28 l/m)
Actual flow will be intermittent.
What type of pump would be suitable for this application?
I welcome suggestions for specific models and suggestions for how to configure the pump.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
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Since years we use diamond knives to cut ultrathin sections of polymers at low temperature (around -150 *C). When cut the sections are floating boat/trough integrated on the diamond knife, from where they are collected.
To cut larger sections we started to make and use glass knives on which a plastic boat/trough is glued using a "Cavex Set Up Wax", a dentistry modelling wax, as suggested by the vendor. Unfortunately the boats fall off at low temperature.
Can anyone suggest a solution or a "glue" that would resist to low temperatures?
Many thanks, Ondrej
==============================Original Headers============================== 6, 41 -- From ondrej.kotecky-at-gmail.com Wed May 4 05:59:00 2016 6, 41 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u44Ax0kR020028 6, 41 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 05:59:00 -0500 6, 41 -- Received: by mail-ig0-f180.google.com with SMTP id bi2so143580313igb.0 6, 41 -- for {microscopy-at-microscopy.com} ; Wed, 04 May 2016 04:01:47 -0700 (PDT) 6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=gmail.com; s=20120113; 6, 41 -- h=mime-version:from:date:message-id:subject:to 6, 41 -- :content-transfer-encoding; 6, 41 -- bh=yHiwbV2Nl3yhgTFlabuEWXh+sc1xNAqG4DWxo1ckEHM=; 6, 41 -- b=fR31egbWeNkAcnAQx2R/TkMkG5bPvJ8keX1dwCVPtJ5rpp240JFRXMy4xaSdgJYtYI 6, 41 -- /JQ9PHNJiwGhi7/UR0jxgz0dAl08EWogwWdDtXnmfTfFWh6lxvYdK/TS0OdlEXlR6g91 6, 41 -- MjYphFDqaDPs9d2NIyitBlcgJlNxhGa/i7U9vEfHCArHCEQDubkZTNFij8uc+hkpPQ9C 6, 41 -- Id9tq+BAvvYpxYCaXovETpq1RvqvxEQd9VFHZLJ3CilDn3IQ6x/wigSFR9DgAtwTu66T 6, 41 -- Vo5+q+iWcDllCAZwSfx7QiM3BH2w6sGEbNxdoBymXx7Q0mZGPtUkWTggzyXFpPgmXDGU 6, 41 -- qw3w== 6, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=1e100.net; s=20130820; 6, 41 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to 6, 41 -- :content-transfer-encoding; 6, 41 -- bh=yHiwbV2Nl3yhgTFlabuEWXh+sc1xNAqG4DWxo1ckEHM=; 6, 41 -- b=K09QTjkxG60A/LJd7Ol014juUHvA102QM1V+5tlBvF7d9z/O83HcYFk12K/UE67iZA 6, 41 -- e+9i+W21EnWxm9mWIGCVsQcgk45kKEP3rnZH8EIBIIuCajkqPIYoIrc4RmP7WoXZX36i 6, 41 -- Kkjz4G4E4MBBEAHM6huqv652y5jk6fRZtA1ogHiu22D+b8FX9AXyQ/eQ/IhOHu0oH3Mt 6, 41 -- 4QCaHHlPNv1/6ZDoAS5n6G+dyBQd0YQNTxArN72MjqY2vRyJCsqdAKqfWeWX6dSHUtKp 6, 41 -- +vOC+Uopx8iSs73L/OJf7M3Tda9srxycYjHr6w5mAno2JjN6CxCvgUUU3SmjHXtVpq5E 6, 41 -- +LRg== 6, 41 -- X-Gm-Message-State: AOPr4FWTFo8zfwP9Pf+8cOGs+ZZobNKpeKOc+pGqU+pxwc1poIQgjKBeWH6blCe1OA+o/H9ZV0pVSkTdXj8bDQ== 6, 41 -- X-Received: by 10.50.10.165 with SMTP id j5mr9842212igb.29.1462359706837; Wed, 6, 41 -- 04 May 2016 04:01:46 -0700 (PDT) 6, 41 -- MIME-Version: 1.0 6, 41 -- Received: by 10.107.26.68 with HTTP; Wed, 4 May 2016 04:01:27 -0700 (PDT) 6, 41 -- From: =?UTF-8?B?T25kxZllaiBLb3RlY2vDvQ==?= {ondrej.kotecky-at-gmail.com} 6, 41 -- Date: Wed, 4 May 2016 13:01:27 +0200 6, 41 -- Message-ID: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com} 6, 41 -- Subject: Cryomicrotomy using glass knives 6, 41 -- To: microscopy-at-microscopy.com 6, 41 -- Content-Type: text/plain; charset=UTF-8 6, 41 -- Content-Transfer-Encoding: 8bit 6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u44Ax0kR020028 ==============================End of - Headers==============================
The Laboratory Technician will be responsible for assisting and/or training scientific staff and students with Scanning Electron Microscopy, X-ray microanalysis, Laser Scanning Confocal Microscopy, Cathodoluminescence Spectroscopy, X-ray Computed Tomography, specimen preparation and image processing and printing; will maintain functionality and capability of lab equipment; will undergo periodic training on new technologies; will order supplies and track usage and expenditures; will interact with curatorial staff and students on diverse projects using microscopy and digital imaging in the fields of zoology, paleontology, anthropology, archeology, and earth and planetary sciences.
SEM (EDS+EBSD+CL) Micro-computed tomography Laser scanning confocal microscopy + Raman spectroscopy TEM
Don: I made a typo in my previous message. The correct model number for the KNF Mini Diaphragm Pump is N 84.3 ANI.
If you decide to use this pump you will need a base plate to mount it on and a housing to shield the cooling fan. I can supply drawings for versions of these items that I'v used previously that are easy and cheap to make.
You will probably also want some vacuum valves, and for this purpose I can recommend the mini ball valves (such as Model 4114T11, T13. T64) sold by McMaster Carr (mcmastercarr.com, 562-692-5911). They also sell a full line of tubing fittings for polyethylene tubing.
Wil Bigelow
==============================Original Headers============================== 4, 42 -- From bigelow-at-umich.edu Tue May 3 09:37:20 2016 4, 42 -- Received: from mail-wm0-f43.google.com (mail-wm0-f43.google.com [74.125.82.43]) 4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u43EbKuj016160 4, 42 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2016 09:37:20 -0500 4, 42 -- Received: by mail-wm0-f43.google.com with SMTP id a17so43346876wme.0 4, 42 -- for {microscopy-at-microscopy.com} ; Tue, 03 May 2016 07:40:04 -0700 (PDT) 4, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 42 -- d=umich.edu; s=google; 4, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 42 -- :content-transfer-encoding; 4, 42 -- bh=s3P39u7sUzezq41+0Cdd4RSU3y7FLKHAYnE1Y2eOeD8=; 4, 42 -- b=YniaPX0DqdyExLahLTBKz+wCF3BtbtqKbFU/bTB0thhQHLnuT2f18hGNr3e6II2jwx 4, 42 -- uBOyPJlyzCNrmoZAr4p6xdyhoUt+kWaP1bZJigYzwdMT1hCxnyuuKj330tcLRalcGp2p 4, 42 -- s/qfgLBfiY0KqUfIkxVAECfZPJibkEEaR5VHM= 4, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 42 -- d=1e100.net; s=20130820; 4, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 42 -- :subject:content-transfer-encoding; 4, 42 -- bh=s3P39u7sUzezq41+0Cdd4RSU3y7FLKHAYnE1Y2eOeD8=; 4, 42 -- b=HSjAPtW0PLQI043x5FQ2xj3LidBNifOMteuyJww2ONQd/y9wJZwQBEaZLcTPZW4SXn 4, 42 -- PncnTEvVxGn+vcwasdemqNbhWNsY0pnlsyT/rp9COqNoC5elLBdyD2ewEelhe1NkbLnc 4, 42 -- JTMUamJMT+0Lbchtypn9ayG29zVY+q8z1qivgHawZZKcRaK6DjpEtjIcE31xSFTP/dhQ 4, 42 -- Xa8aofLh0ZNXGbgmW+xIy5+ydc7oV8bI6KYJA67qOjKmL+GmSs7LRhDanfy/HVEDAkCl 4, 42 -- MEOLxg5WHHkCUj83qZwSvQoAqcb/8pyX5De+or7SaNM3p/gX9utlRIFnMu7G3klaOe1C 4, 42 -- IOHA== 4, 42 -- X-Gm-Message-State: AOPr4FW3MkNjS+nHdbosHY0XMtuLJMgpD8FAPj5P+1ycG8qrmDVRni9dSsAG6kmT5+wuQib9 4, 42 -- X-Received: by 10.28.181.148 with SMTP id e142mr3694442wmf.38.1462286403026; 4, 42 -- Tue, 03 May 2016 07:40:03 -0700 (PDT) 4, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 4, 42 -- by smtp.gmail.com with ESMTPSA id lh1sm4279952wjb.20.2016.05.03.07.40.01 4, 42 -- for {microscopy-at-microscopy.com} 4, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 4, 42 -- Tue, 03 May 2016 07:40:02 -0700 (PDT) 4, 42 -- Message-ID: {5728B83F.5090107-at-umich.edu} 4, 42 -- Date: Tue, 03 May 2016 10:39:59 -0400 4, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 4, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 42 -- MIME-Version: 1.0 4, 42 -- To: microscopy-at-microscopy.com 4, 42 -- Subject: [Microscopy}Correction, Low Vac pump 4, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I think perhaps the Model N 64.3 ANI Mini Diaphragm Pump manufactured by KNF Neuberger (www.knf.com/usa.htm) would fill the bill. It will produce a vacuum below 10 Torr, has a speed of about 4 l//min and costs less than $600. I've used one of these on a couple of systems I've designed in the past with very satisfactory results. Wil Bigelow
==============================Original Headers============================== 1, 42 -- From bigelow-at-umich.edu Mon May 2 21:22:15 2016 1, 42 -- Received: from mail-wm0-f51.google.com (mail-wm0-f51.google.com [74.125.82.51]) 1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u432MECA015382 1, 42 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2016 21:22:15 -0500 1, 42 -- Received: by mail-wm0-f51.google.com with SMTP id g17so25750067wme.0 1, 42 -- for {microscopy-at-microscopy.com} ; Mon, 02 May 2016 19:24:56 -0700 (PDT) 1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=umich.edu; s=google; 1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 1, 42 -- :content-transfer-encoding; 1, 42 -- bh=JVKGanHbZYxc/0P8cM9WHSxyzk+oEpq4CQXApTw9uPs=; 1, 42 -- b=VFnx0Ac/WjRFyShe2yqugmgSZ1rF2qTWz/rJYbsqL4mei5cdH3Z+JCgq8efelChnh+ 1, 42 -- bufGI9XGA5XDn2TDkSk5XqdkdR3wQY5MPLePMUIppS3dnspu3VFRvMa4f0lGvN/wFf6m 1, 42 -- Hw+qjMHQR7rj8I9WCouembRA2tYIx5mjsRv+4= 1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=1e100.net; s=20130820; 1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 1, 42 -- :subject:content-transfer-encoding; 1, 42 -- bh=JVKGanHbZYxc/0P8cM9WHSxyzk+oEpq4CQXApTw9uPs=; 1, 42 -- b=bGFapPthLcKnRh+E3NVxmGaitSj6eNqcDJDo7tiqae9tgzBFJL0/u971gXWILU4+bp 1, 42 -- a0YEkEeqbJw/YiYbIJTJIkNSrRowQeUyKpGNtb79b2kaTLLqW7xtv7LI2EUZzXfnx0f6 1, 42 -- nNn/BiUpRB1rE+1Dl8zYwLdf2IaUoueBfKDuKnlkmEDL+iBQ8pwy2WhAQFiLqNcYd6wV 1, 42 -- +GARn3vUfYsiGGRnkxclQ0JSP7gTWkSAHpVLDSHxQtn3TZCwMcljL/sUx5GwMb41M+Qd 1, 42 -- aWAn9V/XN3a5+tbnDWqGlxNe9XtRkLiy60boDWKQfxLUuUvqE7SMiHsDVejsbOfQ4L6k 1, 42 -- dnQg== 1, 42 -- X-Gm-Message-State: AOPr4FVWpv+K5nkEUb3U+BDQohdidkgMMse7YZI98fylyIszZoQT5QepjQu/Euh3m91JXAju 1, 42 -- X-Received: by 10.194.77.140 with SMTP id s12mr3705105wjw.24.1462242295769; 1, 42 -- Mon, 02 May 2016 19:24:55 -0700 (PDT) 1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 1, 42 -- by smtp.gmail.com with ESMTPSA id d1sm1059056wjb.47.2016.05.02.19.24.54 1, 42 -- for {microscopy-at-microscopy.com} 1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 1, 42 -- Mon, 02 May 2016 19:24:55 -0700 (PDT) 1, 42 -- Message-ID: {57280BF4.3010008-at-umich.edu} 1, 42 -- Date: Mon, 02 May 2016 22:24:52 -0400 1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 42 -- MIME-Version: 1.0 1, 42 -- To: microscopy-at-microscopy.com 1, 42 -- Subject: [Microscopy]Low Vacuum Pump 1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I haven't had to buy a photo printer for micrographs for some years. What's the current best? HP doesn't make the Photosmart series anymore, and the Epson Sure color series specs look good, but ... ? I need both color and greyscale. Maybe the Epson P600? Thanks.
Hi Phil, and colleagues on the list, we have bought an Officejet Pro 8100 two weeks ago, and we have an Officejet Pro 8000 in use since a few years, directly at a TEM with 2k x 2k camera - to the delight of the users. no interest in the manufacturing company, of course ... kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: - 16th Europ Microsc Congress EMC http://www.emc2016.fr/en/ 28 Aug - 2 Sept 2016 in Lyon, FR - Microscopy Conference 2017 Dreilaendertagung Lausanne, CH 20-25 August 2017 - next Microbiol. conferences: VAAM/DGHM - Annual Conf March 5-8, 2017, Wuerzburg
==============================Original Headers============================== 5, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Mon May 2 02:31:45 2016 5, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u427Vjvg031648 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 02:31:45 -0500 5, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with SMTP id 2358A45F18 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 09:34:19 +0200 (CEST) 5, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 0B95F64F18 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 09:34:18 +0200 (CEST) 5, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 5, 25 -- with Novell_GroupWise; Mon, 02 May 2016 09:34:21 +0200 5, 25 -- Message-Id: {57271F18020000540005E385-at-gwsmtp1.uni-regensburg.de} 5, 25 -- X-Mailer: Novell GroupWise Internet Agent 14.2.0 5, 25 -- Date: Mon, 02 May 2016 09:34:16 +0200 5, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 5, 25 -- To: {oshel1pe-at-cmich.edu} , "microscopy listserver" {Microscopy-at-microscopy.com} 5, 25 -- Subject: Re: [Microscopy] Photo printers 5, 25 -- References: {201604291818.u3TIIUhN013081-at-ns.microscopy.com} 5, 25 -- In-Reply-To: {201604291818.u3TIIUhN013081-at-ns.microscopy.com} 5, 25 -- Mime-Version: 1.0 5, 25 -- Content-Type: text/plain; charset=US-ASCII 5, 25 -- Content-Disposition: inline 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u427Vjvg031648 ==============================End of - Headers==============================
I'm working with a wafer inspection system that uses clean dry air to float a vibration isolation table and to release parts from vacuum clamps.
The manufacturer's specifications call for:
Pressure: 80-100 psi
Flow rate: { 1 cfm (28 l/m)
Cleanliness: Filtered to Class I
The actual flow will be intermittent.
I'm looking a quiet system that is easy to maintain. I would like to receive suggestions of specific equipment configurations.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 13, 36 -- From donc-at-asmicro.com Sun May 1 18:05:52 2016 13, 36 -- Received: from resqmta-ch2-05v.sys.comcast.net (resqmta-ch2-05v.sys.comcast.net [69.252.207.37]) 13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u41N5q9g006642 13, 36 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2016 18:05:52 -0500 13, 36 -- Received: from resomta-ch2-18v.sys.comcast.net ([69.252.207.114]) 13, 36 -- by comcast with SMTP 13, 36 -- id x0TCaMHkGBE6Ox0TVauH7k; Sun, 01 May 2016 23:08:29 +0000 13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 13, 36 -- s=q20140121; t=1462144109; 13, 36 -- bh=j0dmGOk5NI5qJ+b0IFtRaN3EYlxluzJp3a1o8e70ji0=; 13, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 13, 36 -- Content-Type; 13, 36 -- b=I8Ar48oxVMDq1oWmHrD21VlZygSVkr8vLAz8SR1AQb4uuI6/OwgzkAR4NzIY9dt9p 13, 36 -- 5Ozzr6L91kRnt0nzyesLNK5XeQ6A7u3lRV4xzJz0hhMsyFxLETe5MlEyKC7NPWnrcQ 13, 36 -- 6uDpkJQw1nx3q+S4SSgGWQBjG51y+F0I2dmbfhb4sFwC5BK9GB7kP3vNfygW8trENm 13, 36 -- x3Epyc0NSbH8IptlPlj0OY1lz0XbuT/tH5cueg7/1HlbQaQDpBF+1Qrm/0J680Q/rP 13, 36 -- bP9CX+yfforOHy5Eit4vqWwaOlaBc4zOABUHWnb1aUvdyfwqEf6Bk3kKW+q2+BR430 13, 36 -- hz3salvOa83Vg== 13, 36 -- Received: from asm20 ([68.45.155.184]) 13, 36 -- by resomta-ch2-18v.sys.comcast.net with comcast 13, 36 -- id pB8V1s0033yyn3Y01B8VdQ; Sun, 01 May 2016 23:08:29 +0000 13, 36 -- Message-ID: {4B176DE84D984487A7C58922082919BD-at-asm20} 13, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 13, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 13, 36 -- Subject: Clean dry air 13, 36 -- Date: Sun, 1 May 2016 19:09:08 -0400 13, 36 -- MIME-Version: 1.0 13, 36 -- Content-Type: text/plain; 13, 36 -- format=flowed; 13, 36 -- charset="iso-8859-1"; 13, 36 -- reply-type=original 13, 36 -- Content-Transfer-Encoding: 7bit 13, 36 -- X-Priority: 3 13, 36 -- X-MSMail-Priority: Normal 13, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 13, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 ==============================End of - Headers==============================
I'm working with a wafer inspection system that uses vacuum to clamp the wafer. The manufacturer's specifications call for a modest vacuum:
low vacuum: 150 torr (-24" Hg)
flow rate: { 1 cfm (28 l/m)
Actual flow will be intermittent.
What type of pump would be suitable for this application?
I welcome suggestions for specific models and suggestions for how to configure the pump.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 10, 36 -- From donc-at-asmicro.com Sun May 1 17:58:18 2016 10, 36 -- Received: from resqmta-ch2-07v.sys.comcast.net (resqmta-ch2-07v.sys.comcast.net [69.252.207.39]) 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u41MwITE003272 10, 36 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2016 17:58:18 -0500 10, 36 -- Received: from resomta-ch2-16v.sys.comcast.net ([69.252.207.112]) 10, 36 -- by comcast with SMTP 10, 36 -- id x0M1axB8sp1Bex0MBa2Ax0; Sun, 01 May 2016 23:00:55 +0000 10, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 10, 36 -- s=q20140121; t=1462143655; 10, 36 -- bh=5YKlv29KfB2X47BOX6fSUaVPqjafQm4cRuCFouKT2o4=; 10, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 10, 36 -- Content-Type; 10, 36 -- b=F4GJAlSZN4QYJMr7WmlCx4WGIS4d5rRo1dsO7Rue/eg6FwvjZ5ciVYBc6uP7IKK2/ 10, 36 -- h/M9ZkU/lqd2QcEJmkfeZhVynFNMY6XHSPTFt3Hg1y8+yc/HVnxtpl+XtqA65SNCxe 10, 36 -- E67BUiY1IvBpRbkwKiiZkaJ9kn6wwf+qLyhD1HuJLAwHS9GCzgbgw9lJMzGteOw0/8 10, 36 -- 43kSF1CKYZep+yn5vN5iCquMZJhPlEOOwFptPRHVhrcbAL7SSoLjDHPFG1MkfBkGcJ 10, 36 -- B4tE+4bxaqdp//lBg331TGmlngEZM31der8NUO0pd9RXJ6tzLLY0oKG8bzMOo4UdbO 10, 36 -- 8glqJjYMhmV0g== 10, 36 -- Received: from asm20 ([68.45.155.184]) 10, 36 -- by resomta-ch2-16v.sys.comcast.net with comcast 10, 36 -- id pB0u1s00C3yyn3Y01B0u4y; Sun, 01 May 2016 23:00:55 +0000 10, 36 -- Message-ID: {EE952F34508646FA93AD17ECFA9405A1-at-asm20} 10, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 10, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 10, 36 -- Subject: low vacuum pump 10, 36 -- Date: Sun, 1 May 2016 19:01:31 -0400 10, 36 -- MIME-Version: 1.0 10, 36 -- Content-Type: text/plain; 10, 36 -- format=flowed; 10, 36 -- charset="iso-8859-1"; 10, 36 -- reply-type=original 10, 36 -- Content-Transfer-Encoding: 7bit 10, 36 -- X-Priority: 3 10, 36 -- X-MSMail-Priority: Normal 10, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 10, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 ==============================End of - Headers==============================
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Email: rebecca.jackson-at-utsouthwestern.edu Name: Rebecca Jackson
Organization: UT Southwestern
Title-Subject: [Filtered] Fume hood extension arm
Message: Hey all- We are looking into getting a fume hood extension/extraction arm in our EM lab, hopefully making it easier and safer for cutting tissues in fix using the dissecting scope. We hope to do this on the bench with the arm right next to the dish containing the tissue and fix while using the dissecting scope. Does anyone have experience with these? Could I get some info on what you have or if these even work well for harsh chemicals? Or do you do/use something totally different....? Thanks all!!
-Bec.
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We have a student, that would like to work with a polymer that if 100% polymerized would be ok, but seldom are polymer's 100% polymerized. In other physical states this polymer is toxic.
They hope to make this into a thin film, and use it in x-ray, SEM and TEM.
The toxin/ sensitizer is ethylviologen (ethyl iodide), a bio based substrate known to be a sensitizer, the polymer has the base of Polyvinylbenzylchloride, 4-4-bipyridine, and could include ethylviologen monomers and dipyridyl monomers.
SUGGESTED PRECAUTIONS ALREADY: Vacuum for a week, mild heat to help gas off, buying own sample stubs and bringing already prepped. Test just plain polybenzylchloride to watch for sublimation.
Desired instruments of use: SEM, TEM, X-ray
QUESTIONS:
* Even if vacuum is pre-applied for as long as a week in their own lab, will such a product gas out into the TEM causing all the insides to be contaminated?
* Even if vacuum is not a problem, what about sublimation of the polymer by the beam?
* What are your standard precautions for soft polymers, especially those that are toxic? What are the limits of materials you allow in your EM scopes?
Thanks for any help,
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
I haven't had to buy a photo printer for micrographs for some years. What's the current best? HP doesn't make the Photosmart series anymore, and the Epson Sure color series specs look good, but ... ? I need both color and greyscale. Maybe the Epson P600? Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 33 -- From oshel1pe-at-cmich.edu Fri Apr 29 13:17:59 2016 3, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TIHx48012606 3, 33 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 13:17:59 -0500 3, 33 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 3, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3TIKRCS001795 3, 33 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 14:20:27 -0400 3, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 3, 33 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 3, 33 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 14:20:28 -0400 3, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 3, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 3, 33 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 14:20:27 -0400 3, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 3, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 29 Apr 3, 33 -- 2016 14:20:27 -0400 3, 33 -- Message-ID: {5723A5EB.8070708-at-cmich.edu} 3, 33 -- Date: Fri, 29 Apr 2016 14:20:27 -0400 3, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 33 -- MIME-Version: 1.0 3, 33 -- To: micro {microscopy-at-microscopy.com} 3, 33 -- Subject: Photo printers 3, 33 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 33 -- Content-Transfer-Encoding: 7bit 3, 33 -- X-Originating-IP: [141.209.2.100] 3, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 33 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 3, 33 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 33 -- X-CanItPRO-Stream: default 3, 33 -- X-Canit-Stats-ID: 02QMikr4G - e511e4dc73f0 - 20160429 3, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
LaB6 enthusiasts! This afternoon I am looking at 4 Kimball filaments in the FIB. Some had a dignified normal death, others an untimely and less dignified death. What we are going to look at is the elemental differences between them, from the La right to the base. We are going to do slice and views and look for defects. Is there anything else you guys want us to investigate? Let me know and your dreams will come true, we are the Disneyland of microscopy.
I have been contacted by one of the manufacturers, they too are interested in the data we get. So things are moving and hopefully we will be getting good product in the near future.
Next on my agenda will be the quality and performance of FEG tips. How have your FEG tips been lately?? We at Cornell have tales to tell. Send me your tales of woe or post them, but let s just say our experience lately has been similar to our issues with LaB6.
Thanks for interacting, and if we don t keep the suppliers on their toes, our down time because of poor product will just be expected rather than a surprise.
jg
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Fri Apr 29 11:24:14 2016 7, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TGOELT012018 7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 11:24:14 -0500 7, 30 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged)) 7, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3TGQgkq020469 7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 12:26:42 -0400 7, 30 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 7, 30 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 7, 30 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 12:26:43 -0400 7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 7, 30 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 29 Apr 7, 30 -- 2016 12:26:42 -0400 7, 30 -- Message-ID: {57238B42.7080700-at-cmich.edu} 7, 30 -- Date: Fri, 29 Apr 2016 12:26:42 -0400 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 30 -- MIME-Version: 1.0 7, 30 -- To: micro {microscopy-at-microscopy.com} 7, 30 -- Subject: Fwd: LaB6 Update: Could use some suggestions 7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-Originating-IP: [141.209.2.100] 7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 7, 30 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 02QMgqGeS - 8b3b80445ea6 - 20160429 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: jzheng-at-uci.edu Name: Jian-Guo Zheng
Organization: IMRI UCI
Title-Subject: [Filtered] Project Scientist Series Electron Microscopy Scientific Staff -positions open at UC Irvine
Message: For details, please visit https://recruit.ap.uci.edu/apply/JPF03381
RECRUITMENT PERIOD Open April 20th, 2016 through June 30th, 2016 DESCRIPTION
Project Scientist Series -Electron Microscopy Scientific Staff
Salary-Commensurate with experience
POSTING DATE: APRIL 20, 2016 CLOSING DATE: OPEN UNTIL FILLED
DESCRIPTION Manage the day-to-day operations and help guide the strategic build-out of the new transmission electron microscopy (TEM) facility at the UC Irvine Materials Research Institute (IRMI). IMRI has a large and growing user community of over 300 academic and industry users, with advanced materials characterization instruments that include state-of-the-art TEMs, scanning electron microscopes (SEMs), focused ion beam (FIB) systems, and a Kratos AXIS Supra surface science instrument (for details see http://lexi.eng.uci.edu). IMRI has recently built a new TEM facility housing five major instruments, including four high-end TEMs (Nion UltraSTEM 200 HERMES, JEOL JEM-ARM300CF Grand ARM, JEM-2800, and JEM-2100F Cryo-TEM) and a dual-beam FIB (Tescan GAIA-3 XMH FIB-SEM). IMRI will be the first lab in the Americas with the newly introduced JEOL Grand ARM and the Nion s high-performance UltraSTEM 200 HERMES, which provides an energy resolution for electron energy loss spectroscopy (EELS) of 7 meV or better. Both the Grand ARM and the 2100F Cryo-TEM will be equipped with Gatan s K2 cameras. IMRI is also equipped with a variety of TEM in situ holders and specimen preparation tools. Two TEM scientific staff positions are available: (1) TEM specialist for materials science applications and (2) Cryo-TEM specialist for biological and soft materials. The successful applicant must hold PhD or equivalent degrees in the areas of Materials Science, Physics, Chemistry or biology, and be TEM experts with at least five years of in-depth, hands-on experience with TEM/STEM imaging, spectroscopy, TEM sample preparation, and maintenance of TEM equipment. Applicants who are merely users of TEM will not be considered. The applicant must have mastery of modern TEM/STEM instrumentation and methods, data collection, analysis, and interpretation. Candidates with experience operating user facilities and expertise with advanced TEM/STEM imaging, spectroscopy, and in-situ microscopy techniques are of particular interest. Duties include: operate, maintain, and improve the TEMs; work with the IMRI leadership to identify and obtain accessories and additional equipment for a phased build-out of the TEM facility; train, supervise, and work directly with users to carry out research; co-manage all aspects of day-to-day IMRI operations; participate in teaching and outreach activities; promote IMRI on and off campus; secure grants, perform original research, attend scientific conferences, and publish scientific research in peer-reviewed journals. The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy. TO LEARN MORE AND APPLY Apply by submitting your application to our online RECRUIT system at: https://recruit.ap.uci.edu/apply/JPF03381 More information about this recruitment: www.calit2.uci.edu JOB LOCATION Irvine, CA REQUIREMENTS DOCUMENTS Curriculum Vitae - Your most recently updated C.V. Cover Letter Statement of Research Statement of Teaching (Optional) Statement of Contributions to Diversity - Statement addressing how past and/or potential contributions to diversity will advance UCI's Commitment to Inclusive Excellence. Misc / Additional (Optional) REFERENCES 3-5 letters of reference required HOW TO APPLY 1. Create an ApplicantID 2. Provide required information and documents 3. If any, provide required reference information
To apply these positions, please visit https://recruit.ap.uci.edu/apply/JPF03381 Thanks!
Jian-Guo Zheng, PhD, FRMS IMRI Director of Facilities Irvine Materials Research Institute University Of California, Irvine 3421 Calit2 Building Irvine, CA 92697-2800 USA Telephone: 949-824-0441 (office), 949-468-9980 (cell) Fax: 949-824-8195 Email: jzheng-at-uci.edu
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I've already sent you a manual for the EMS 850 CPD but can give you some pointers that are not included in the manual.
What CO2 tank size do you use? Typically your gas supplier will deliver a J-size tank but it MUST have a "Dip tube or Siphon tube" for any CPD to function. The siphon tube draws liquid Co2 from the bottom of the tank, without this you would only draw gas.
What support items do you use? Plastic waste containers to contain Alcohol waste. Small plastic flowmeter to measure the flow of Co2 out of the pressure vessel during the final stage of drying. There are several types of sample holders depending on application, Everything from bulk samples to coverslip holders are in the EMS 850 section of our catalog.
This unit comes with a SST reinforced, 1500psi certified transfer hose. If you didn't get one with the used 850, contact me directly for a replacement.
How automatic is the system? The EMS 850 is semi-automatic. See the details of operation in the manual.
For putting bio specimens into the SEM, how do you factor-in this CPD before going into the chamber? Not sure I understand what the question is. All biological samples must be dried* before coating or insertion into the SEM
Al Coritz (acoritz-at-emsdiasum.com) Applications & Service Manager EMS Technical Service
www.emsdiasum.com 800-523-5874 1560 Industry Road Hatfield, PA 19440
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists I have a follow up on our LaB6 issue and a suggestion that should work from one of our microscopy luminaries.
There is correspondence going out to Denka and Kimball regarding our issues, with our concerns and observations attached, from one of the major suppliers of microscopy consumables. Hopefully there will be some movement or at least an acknowledgement that Oh yeah, we did change our La supplier and welding technique and who we are outsourcing manufacturing to . Don t think you are the only one having issues guys! Throw something up on the list and at the least you will get that reality check that you are not losing your mojo, or the reality check that indeed you ARE losing your mojo.
Regarding the manual alignment phenomena; if you recall, we align the Lab6 on the bench as usual, put the gun in the scope, heat it up and the deflectors can t center the tip, we max out. Take the gun out and the manual alignment looks great. Henk Colijn theorizes that the resistance is not the same on the posts so as they heat up the tip gets pulled to one side or the other. Earl Kirkland suggested that we anneal the filament before we manually align, which is what we do for our cold FEGs. I gave it a go with my latest Lab6: ran the HT to 120kV and ran a dribble current through the crystal over the weekend, getting it good and hot, manually aligned and so far so good. BUT we should not have to do this because in the recent past we never had to do this. In our case it is more downtime which ultimately adds to the cost of the filament.
So guys, I will keep you posted on what happens, any other observations or suggestions please pass on to our community. Some issues may seem trivial but we all have a common bond, and our collective mojo is a pretty awesome force.
jg
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Thu Apr 28 08:45:50 2016 7, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3SDjoFx009525 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 08:45:50 -0500 7, 30 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3SDmDod000479 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 09:48:14 -0400 7, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 7, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 30 -- (TLS) id 14.3.248.2; Thu, 28 Apr 2016 09:48:15 -0400 7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 7, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 28 Apr 7, 30 -- 2016 09:48:14 -0400 7, 30 -- Message-ID: {5722149E.7020302-at-cmich.edu} 7, 30 -- Date: Thu, 28 Apr 2016 09:48:14 -0400 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 30 -- MIME-Version: 1.0 7, 30 -- To: micro {microscopy-at-microscopy.com} 7, 30 -- Subject: Fwd: LaB6 update and some suggestions 7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-Originating-IP: [141.209.2.100] 7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: -1.70 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 7, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 05QLNMdBw - f6f14fd4f1be - 20160428 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Current EM Techniques Workshop on May 24 & 25
Message: Dear Colleagues,
We would like to remind you that the registration of the UMB Current EM Techniques Workshop (05/24-05/25) is OPEN. The purpose of this annual workshop is to introduce participants to new electron microscopy techniques and instruments via live demonstration and open discussion. The focus of this year s workshop is Correlative LM & EM. The workshop will include oral presentations, Tips-and-Tricks Technology forums on the first day and live instrument demonstration on the second day. Dr. Lucy Collison of Francis Crick Institute and Richard Schalek of Harvard University will be the keynote speakers. Demo instruments will feature ATUMtome of RMC-Boeckeler, ASP-1000-IGL of Microscopy Innovations, Pelco Biowave Pro Microwave System of TedPella, Cryo EM sample preparation of Leica Microsystems, Correlative Array Tomography of Zeiss, Polyo III, Lynx Tissue Processor of EMS, Aerosurf Atmospheric SEM of Hitachi/Angstrom.
A joint dinner event with the Chesapeake Microscopy and Microanalysis society (CMMS) on May 24th provides opportunities for social and scientific exchange among workshop participants and CMMS members.
More information: http://www.dental.umaryland.edu/2016currentemtechniquesworkshop/
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I am preparing mouse livers for TEM the classical way: fixation glutar+Formaldehyde, Osmium post-fixation, embedding in Epon. Some liver samples are loaded with fat, mainly due to a treatment the mice received. The sample were left several weeks in fixative at 4*C before processing so extraction of at least some fat would not be a surprise.
Now on semithin sections stained with methylene blue/Azur II the fat globules have a greenish blue appearance. I thought it was due to the presence of osmium. However, when I observe ultrathin sections of the same samples in TEM, the fat globule are empty, I see nothing, it is only resin inside so they are probably extracted. My question is: how can the fat be colored in semi-thin sections and empty/extracted in ultrathin section? How do you explain de greenish blue color with methylene blue/azur II?
Regards, Stephane
==============================Original Headers============================== 5, 30 -- From nizets2-at-yahoo.com Thu Apr 28 02:56:20 2016 5, 30 -- Received: from nm32-vm7.bullet.mail.bf1.yahoo.com (nm32-vm7.bullet.mail.bf1.yahoo.com [72.30.239.143]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3S7uJFJ019541 5, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 02:56:20 -0500 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1461830324; bh=JKYEh/Nb7z1jDUPskNBJleAOwbR37AR7SCKdl0LaPa0=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c+Tr9DwF6k6g0MSWRJs0oTTwD9VminoLotDyXjl8x8VyOjB60zpdSbGv4HbnnCbuv/FjR1ntzKbpB6yIYL5hlWHSkMCBN0TIhOg48TtKTw5C2vTc6evUUNN1A3P3IVRikTjFhZU019eIMZZBHz+yCc5+tJGbAxwRj5B86NMDX9YoL0Av5blnrLaAfmgujCHIa4GHbBoFiLz/fEXsyBkDV4tCWf8E1UTNUJTdvPWsZ6ryrq2LyM8R7z7lDwYWSQlaNq1aDkMqD6rO6S59sNUH6YPbrPGwYaWKXPQtQUngCi/3dTvjXzEwyYJjXb3byTCmwy/6EOUTsfaGTgiVDuUD7Q== 5, 30 -- Received: from [98.139.214.32] by nm32.bullet.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000 5, 30 -- Received: from [98.139.212.211] by tm15.bullet.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000 5, 30 -- Received: from [127.0.0.1] by omp1020.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000 5, 30 -- X-Yahoo-Newman-Property: ymail-3 5, 30 -- X-Yahoo-Newman-Id: 33956.30784.bm-at-omp1020.mail.bf1.yahoo.com 5, 30 -- X-YMail-OSG: qXIR1BcVM1m2zt7pjvnoeFCGSPKW_3KeP3rfp5ylNjkSbyAxs3InvvHhp7u7ONR 5, 30 -- 8RsKBerL6w74Ih5C740SC273MEAEOfv4LP_e01qu5WwdnGR8ogSZ6oHFQAJgJkFbXTha_eH8Rs7m 5, 30 -- AxHtg4EI7Pk6kzu5kIg24Ou7op6Es213edNdZOIA54H.Ew8VVwdzkExhbh1t18lKfaxWeUkNdLZI 5, 30 -- Y9mZRezrl8SzpYX1DGeJ_zC3wVz0MdHSHbpaG5U5U5EkRh5lxmpuNjXwJLSUus_Se5IbAw7exYrK 5, 30 -- zFZfI8OYGJAMDXS1oDhA47zwCMwtQXiUv282VJ7tJeKoWTBaGDvMDtAIJjb.fqIt6uEHHK2z0T1R 5, 30 -- X.j2VKFBbaJvQS.OqoPmItEFJCPRk8zocQMtLXV7gVRC_iXeoZOdz6PcgS6Ywf6ARcWCT3zP1bVh 5, 30 -- 93e8RVO7m34gvhnW4LThGZ6vj92gJYvurGyh.Q32ErY9yiVTNVufv.3IdebZ4M.B5FQocnuirzZO 5, 30 -- PD83R 5, 30 -- Received: by 66.196.80.122; Thu, 28 Apr 2016 07:58:43 +0000 5, 30 -- Date: Thu, 28 Apr 2016 07:58:43 +0000 (UTC) 5, 30 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 30 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 5, 30 -- To: {microscopy-at-microscopy.com} 5, 30 -- Message-ID: {1594685055.3925265.1461830323263.JavaMail.yahoo-at-mail.yahoo.com} 5, 30 -- Subject: preparation of fat in mouse liver specimen 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; charset=UTF-8 5, 30 -- References: {1594685055.3925265.1461830323263.JavaMail.yahoo.ref-at-mail.yahoo.com} 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3S7uJFJ019541 ==============================End of - Headers==============================
I'm tring to get a used EMS850 CPD up and running and wonder what others' experiences have been with this unit. This unit is before the version with a single dial gauge and digital readout.
What CO2 tank size do you use? What support items do you use? What hose works for you? How automatic is the system?
For putting bio specimens into the SEM, how do you factor-in this CPD before going into the chamber?
All input is appreciated.
TIA, gary g.
==============================Original Headers============================== 6, 26 -- From gary-at-gaugler.com Wed Apr 27 20:31:08 2016 6, 26 -- Received: from wavecable.net (cgfe-34-tuk.wavecable.net [66.235.59.183]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3S1V8IJ017836 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 20:31:08 -0500 6, 26 -- Received: from [172.16.141.11] (HELO wavecable.net) 6, 26 -- by cgfe-3-tuk.wavecable.net (CommuniGate Pro SMTP 6.1.8) 6, 26 -- with ESMTP id 4213441 for microscopy-at-microscopy.com; Wed, 27 Apr 2016 18:26:38 -0700 6, 26 -- X-Junk-Score: 0 [] 6, 26 -- X-Cloudmark-Score: 0 [] 6, 26 -- X-Cloudmark-Analysis: v=2.1 cv=UdusM/mN c=1 sm=1 tr=0 a=2VnlTcbn8LW/DKys2mm+zQ==:117 a=IkcTkHD0fZMA:10 a=SEOrLOue2igA:10 a=kziv93cY1bsA:10 a=3T1MYC1FAxPeSyEvPIUA:9 a=QEXdDO2ut3YA:10 6, 26 -- Received: from [192.92.0.19] (HELO [127.0.0.1]) 6, 26 -- by wavecable.com (CommuniGate Pro SMTP 6.1.8) 6, 26 -- with ESMTP id 76122850 for microscopy-at-microscopy.com; Wed, 27 Apr 2016 18:26:38 -0700 6, 26 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 26 -- Subject: EMS850 CPD use 6, 26 -- Reply-To: gary-at-gaugler.com 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Message-ID: {57216861.8000008-at-gaugler.com} 6, 26 -- Date: Wed, 27 Apr 2016 18:33:21 -0700 6, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 6, 26 -- Thunderbird/38.3.0 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- X-Antivirus: avast! (VPS 160427-0, 04/26/2016), Outbound message 6, 26 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
Hi Everyone, We are planning a field canceling system for the room where we will house an FEI Talos. There is the possibility of building the coils into the microscope enclosure - has anyone done that? Looking to hear experiences.
Thanks
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
==============================Original Headers============================== 7, 33 -- From gilpin-at-purdue.edu Wed Apr 27 16:03:43 2016 7, 33 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3RL3hZg025450 7, 33 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 16:03:43 -0500 7, 33 -- Received: from exchange.purdue.edu (exchange.purdue.edu [128.210.1.29]) 7, 33 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id u3RL65n9028670 7, 33 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 17:06:05 -0400 7, 33 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl 7, 33 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1156.6; Wed, 27 Apr 7, 33 -- 2016 17:06:05 -0400 7, 33 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 7, 33 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 7, 33 -- 15.00.1156.000; Wed, 27 Apr 2016 17:06:05 -0400 7, 33 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 33 -- Subject: Field canceling systems in a Talos enclosure 7, 33 -- Thread-Topic: Field canceling systems in a Talos enclosure 7, 33 -- Thread-Index: AdGgyF5K4LgsYozqReCcJVLTt1j3RQ== 7, 33 -- Date: Wed, 27 Apr 2016 21:06:04 +0000 7, 33 -- Message-ID: {0d66719e56744cf3bcefdb215588672d-at-wppexc03.purdue.lcl} 7, 33 -- Accept-Language: en-US 7, 33 -- Content-Language: en-US 7, 33 -- X-MS-Has-Attach: 7, 33 -- X-MS-TNEF-Correlator: 7, 33 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 33 -- x-originating-ip: [10.163.23.208] 7, 33 -- Content-Type: text/plain; charset="utf-8" 7, 33 -- MIME-Version: 1.0 7, 33 -- X-PMX-Version: 6.0.2.2308539 7, 33 -- X-PerlMx-URL-Scanned: Yes 7, 33 -- X-PerlMx-Virus-Scanned: Yes 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u3RL3hZg025450 ==============================End of - Headers==============================
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Email: james.weston-at-nyu.edu Name: James Weston
Organization: New York University Abu Dhabi
Title-Subject: [Filtered] Electron microscopy position open
Message: Hello, We have recently posted a position for a research instrumentation specialist in electron microscopy.
This is a permanent staff position. The individual would be responsible for three electron microscopes (SEM, TEM, Dual beam).
Please pass on the announcement to colleagues who are not subscribers to this list service
Thanks! James
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS and EFTEM Workshop at Harvard University
Message: 10:00 am - 6:00 pm, May 24, 2016 Center for Nanoscale Systems (CNS), Room 303, Laboratory for Integrated Science and Engineering (LISE) Building, Harvard University, MA
This workshop combines presentations and extensive hands-on training on advanced EELS and EFTEM techniques and applications in the (S)TEM microscope. Practical experience with EELS is highly recommended. Participants will learn best practices to set up and optimize their EELS hardware and experimental protocols so they can capture and extract the maximum amount of compositional and chemical information from their TEM samples.
Topics include:
-Advanced EELS acquisition techniques extended dynamic range and DualEELS -Advanced EELS processing techniques, MLLS and NLLS fitting -Aberration correction -Quantification using EELS -Ultrafast compositional mapping -High energy resolution chemical mapping and identification of different chemical phases -High energy resolution EELS STEM analysis -Atomic resolved level analysis using EELS -How to best optimize the spectrometer and increase the sensitivity -How to set up the microscope to get the best EELS results
This event is free to registered participants. Register online at: http://www.gatan.com/company/events/advanced-eels-and-eftem-workshop-harvard-university
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Title-Subject: [Filtered] staff scientist position
Message: Staff Scientist Position
The Laboratory of Cell Structure and Dynamics (LCSD) in the Division of Intramural Research (DIR), National Institute on Deafness and Other Communication Disorders (NIDCD), National Institutes of Health (NIH), seeks a Staff Scientist.
The LCSD seeks an integrated molecular understanding of the architecture, dynamics, function, and renewal of specialized cellular structures underlying the mechanosensory function of auditory and vestibular sensory hair cells. Current projects at the LCSD involve the use of a combination of cell biology approaches with super-resolution fluorescence microscopy and cryo-electron microscopy to examine the molecular architecture of the actin-based sensory stereocilia and their mechanotransduction molecular complex.
The NIDCD intramural program is comprised of a highly interactive and dynamic group of scientists at the forefront of research on communication disorders. The LCSD is located in the newly constructed, multi-disciplinary Porter Neuroscience Research Center on the main NIH campus in Bethesda, Maryland.
The LCSD Staff Scientist serves as a laboratory leader who contributes to mentoring of postdoctoral fellows and other laboratory staff and trainees. The position requires outstanding interpersonal skills, teamwork, and excellent oral and written communication abilities. The successful candidate should have a doctoral degree, postdoctoral research experience, and an outstanding publication record. Requisite expertise includes molecular and cellular biology, and either advanced fluorescence microscopy imaging or CryoEM. Salary is commensurate with experience.
Applicants should submit their curriculum vitae with bibliography, statement of interest, and names and contact information for three references to Bechara Kachar at kacharb-at-nidcd.nih.gov. Applications will be reviewed starting May 15, 2016, and accepted until the position is filled. HHS, NIH, and NIDCD are Equal Opportunity Employers. For more information about the LCSD and NIDCD, please visit our website: https://www.nidcd.nih.gov/about/staff/bechara-kachar-md https://www.nidcd.nih.gov/research/labs/section-structural-cell-biology
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Email: akgadde-at-mix.wvu.edu Name: akshitha
Organization: West Virginia University
Title-Subject: [Filtered] Image Processing
Message: Can someone help me understand in detail how the image processing works in background in a scanning electron microscope. How an image is produced from the current signal input to the image processing input in a scanning electron microscope.
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Announcing the Fall Biological Conference at the University of Illinois Materials Research Lab.
DATE: November 2-3, 2016
Conference Title: Diverse Fields of Study... Steps toward Medicine. ________________________________________ An apropos title, as UIUC is starting up its own medical school, MD/PHD with the PhD in Bioengineering.
Attendee registration to open in June and is open to scientists from all over. =========================================================
Vender registration is open NOW. ==========================
Student and Post Doc Presentation Divisions Competition: ============================================= up to 4 post docs, up to 9 students Register from June to October 1st, and upload 1/2 page abstract that will be judged by a panel for acceptance. Winners receive monetary awards, registration waved, and their image on our website for several months. Students as well as post docs from other institutions are welcome.
Invited Speakers: ==============
Our student competition: up to 4 post docs, up to 9 students.
Dr. Catherine Murphy, Chemistry, MRL associate director Gold Nanoparticles: Physics, Chemistry, Biology and Ecology
Adele Akhtar of Psionic, The Future of Upper Limb Prosthetic Devices, Doctoral Candidate Adele Akhtar owns a company that works with prosthetics that can sense touch pressure.
Dr. Ralph Nuzzo - Chemistry/ MRL about Hydrogel and cells
Dr Amy Wagoner - Johnson MechSci Biomaterials, bone implants
Dr Rashid Bashir - Bioengineering.
Dr. Mauro Sardela our new MRL director of the MRL Central Research Facilities, covering what our facility is all about.
Also a representative from our Safety department, topic to be determined.
VENDORS: ===========
This year we have 2 full days of conference,and we have some treats for our vendors. the second day. The vendor area will be open to the public, so this will give you a chance to set up demos and schedule tutorials at your table. Also, you can invite your contacts to come and visit you, regardless of if they have registered. So the second day is public and yours to add visitors, tutorials etc, as you may wish.
The second thing we would like to do for you is give you a chance to speak at the conference. When registering, you can upload a one page abstract to submit, images welcome. This should be in the form of a teaching tutorial. We were able to carve out some time, the 4 best panel judged abstracts will be able to give a 30 minute talk on the second day.
Abstract Deadline for the abstract submission is July 4th, 2016, and folks will know one way or the other by July 10th.
Registration deadline is closer to the meeting, but will end if all tables are full.
There is no fee for the chance to speak, and for 2 full days of tables, the price is $250/ table.
Registration is now open at: "MRL Biological Conference 2016 Exhibitor Registration" https://my.mrl.illinois.edu/eventreg/register.asp
Thanks! More information at the start of registration for attendees is to come.
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
LaB6 users, I am not losing my touch and there is no LaB6 death vortex hanging over Ithaca! I have a whole slew of responses that concur with the issues I have been having. If any of you who are having the same issue please get in touch with me because if we don t tell the vendors and the suppliers that their product has changed for the worse they will still keep selling us garbage because either they don t know or just don t care. BTW, not one retort from Kimball or Denka yet.
I think all we want is a LaB6 that shows us a Maltese Cross, doesn t drift X-Y & Z to the point that the deflectors can t compensate (this is a common issue, you take the gun out and the tip still looks perfectly centered, you apply a current and it tilts toward the Emerald City), and a long life; just the way they used to be ten years ago.
I can t make it to M&M this year or next, that s when the ankle bracelet comes off, but this may be a good topic for a round table because we the users are not imagining things.
Send me some more horror stories, I will compile them and post them or you guys just throw them up on the Microscopy list, do not respond to Phil, I may owe him a keg if you reply to him.
jg
John L. Grazul {jlg98-at-cornell.edu}
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Thu Apr 21 13:09:47 2016 7, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3LI9lt5003974 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 13:09:47 -0500 7, 30 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3LIBkX7008383 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 14:11:47 -0400 7, 30 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 7, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 30 -- (TLS) id 14.3.248.2; Thu, 21 Apr 2016 14:11:47 -0400 7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 7, 30 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 21 Apr 7, 30 -- 2016 14:11:46 -0400 7, 30 -- Message-ID: {571917E2.1040407-at-cmich.edu} 7, 30 -- Date: Thu, 21 Apr 2016 14:11:46 -0400 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 30 -- MIME-Version: 1.0 7, 30 -- To: micro {microscopy-at-microscopy.com} 7, 30 -- Subject: Follow up to LaB6 Issues 7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-Originating-IP: [141.209.2.100] 7, 30 -- X-Bayes-Prob: 0.9792 (Score 4, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: 2.80 (**) [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.9792:4.0) 7, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 05QJ6bLCS - d89b668dac04 - 20160421 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
We too have had problems with Denka LaB6 on our FEI T20. Short life and bad drift. We have to take the wehnelt out several times to re-center the LaB6 as it runs out of gun tilt. We are experimenting with whether to just turn down the filament current for overnight or if it is best to turn it off completely. After a long spell of just turning it lower we are now turning it on in the morning and off at the end of the day. Its early days but I think we have better lifetime. Next time we change filament we have plans to try a different cathode assembly.
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Wednesday, April 20, 2016 4:19 PM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn't imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can't compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn't cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu} --
==============================Original Headers============================== 6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500 6, 33 -- Received: from cas.cmich.edu ([141.209.15.90]) 6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 6, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Wed, 20 Apr 6, 33 -- 2016 16:00:39 -0400 6, 33 -- Message-ID: {5717DFE6.2060309-at-cmich.edu} 6, 33 -- Date: Wed, 20 Apr 2016 16:00:38 -0400 6, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 33 -- MIME-Version: 1.0 6, 33!
We to have been having the same problem with Denka LaB6 filaments on a JEM-3010. We have taken the last two out after aligning them because they appear to be tilted only to find that they are perfectly aligned!
Over time the last one got better and we got closer to the Maltese cross . We have not seen a sharp decline in lifetime however.
Regards
Alan
On 4/20/2016 3:00 PM, oshel1pe-at-cmich.edu wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu}
-- Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110, Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Tel: 312 996 1227
Web site http://www.rrc.uic.edu/ems Wiki site https://wiki.rrc.uic.edu/wiki/EMS
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Hello Henk Sorry, my experience is only with Tungsten but what about if you position the cathode a little bit shifted to the opposite direction you suspect it shifts after warming. So you may correct the problem and verify your theory at the same time.. regards yorgos
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, April 21, 2016 3:56 AM To: eikonika-at-otenet.gr
Hi John, et al,
I've had some issues over the past several years with the Kimball Physics LaB6 cathodes. (I'm not sure which brand you are using.) After meticulously setting the depth and centering the cathode, I run out of gun tilt when warming it up. After pulling it back out of the scope, I find that the cathode is still perfectly centered in the wehnelt. I often go through 3 or 4 iterations before I can get a usable setting of the cathode, each time finding that it looks perfectly centered outside of the scope.
My suspicion is that the legs have slightly different resistances and that one leg warms up more than the other pushing the cathode to the side. When the cathode cools back down, the tip recenters itself. This theory is pretty hard to verify since I can't directly observe the tip while it is warm. If I can get the tip centered enough I do see the "maltese cross" figure though sometimes it's a bit off center.
One thing that may affect your lifetime is the filament limit for saturation. I have noticed that if I initially set the cathode to be just saturated, then check it 20-30 minutes later I find I can back the filament drive off by 1 or 2 clicks and it is still saturated. I assume that as the components warm up, the saturation point changes slightly. I do have to tell the students that this is the correct way to operate so that they don't reset the limit themselves. So, I now set my filament limit to be at least one click undersaturated. This way, as it warms up, it will bring itself to the proper saturation point. Note that the Kimball Physics documents indicate that the evaporation rate will triple in going from 1800K to 1850K. This will bring the lifetime from ~2000 hours down to a 600-700 hour range.
If the tip isn't oxidized by one of the students, I can still get close to 2000 hours from a cathode. I did have one student destroy one in 20 minutes by letting the cold trap warm up while it was hot. (I was NOT happy.) Kimball Physics has some useful info in the technical documents on their web site.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: oshel1pe-at-cmich.edu To: colijn.1-at-osu.edu Sent: 4/20/2016 4:01:46 PM
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn?t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can?t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn?t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu} --
==============================Original Headers============================== 6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500 6, 33 -- Received: from cas.cmich.edu ([141.209.15.90]) 6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with
Hello Henk Sorry, my experience is only with Tungsten but what about if you position the cathode a little bit shifted to the opposite direction you suspect it shifts after warming. So you may correct the problem and verify your theory at the same time.. regards yorgos
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Thursday, April 21, 2016 3:56 AM To: eikonika-at-otenet.gr
Hi John, et al,
I've had some issues over the past several years with the Kimball Physics LaB6 cathodes. (I'm not sure which brand you are using.) After meticulously setting the depth and centering the cathode, I run out of gun tilt when warming it up. After pulling it back out of the scope, I find that the cathode is still perfectly centered in the wehnelt. I often go through 3 or 4 iterations before I can get a usable setting of the cathode, each time finding that it looks perfectly centered outside of the scope.
My suspicion is that the legs have slightly different resistances and that one leg warms up more than the other pushing the cathode to the side. When the cathode cools back down, the tip recenters itself. This theory is pretty hard to verify since I can't directly observe the tip while it is warm. If I can get the tip centered enough I do see the "maltese cross" figure though sometimes it's a bit off center.
One thing that may affect your lifetime is the filament limit for saturation. I have noticed that if I initially set the cathode to be just saturated, then check it 20-30 minutes later I find I can back the filament drive off by 1 or 2 clicks and it is still saturated. I assume that as the components warm up, the saturation point changes slightly. I do have to tell the students that this is the correct way to operate so that they don't reset the limit themselves. So, I now set my filament limit to be at least one click undersaturated. This way, as it warms up, it will bring itself to the proper saturation point. Note that the Kimball Physics documents indicate that the evaporation rate will triple in going from 1800K to 1850K. This will bring the lifetime from ~2000 hours down to a 600-700 hour range.
If the tip isn't oxidized by one of the students, I can still get close to 2000 hours from a cathode. I did have one student destroy one in 20 minutes by letting the cold trap warm up while it was hot. (I was NOT happy.) Kimball Physics has some useful info in the technical documents on their web site.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: oshel1pe-at-cmich.edu To: colijn.1-at-osu.edu Sent: 4/20/2016 4:01:46 PM
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn?t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can?t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn?t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu} --
==============================Original Headers============================== 6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500 6, 33 -- Received: from cas.cmich.edu ([141.209.15.90]) 6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with
I've had some issues over the past several years with the Kimball Physics LaB6 cathodes. (I'm not sure which brand you are using.) After meticulously setting the depth and centering the cathode, I run out of gun tilt when warming it up. After pulling it back out of the scope, I find that the cathode is still perfectly centered in the wehnelt. I often go through 3 or 4 iterations before I can get a usable setting of the cathode, each time finding that it looks perfectly centered outside of the scope.
My suspicion is that the legs have slightly different resistances and that one leg warms up more than the other pushing the cathode to the side. When the cathode cools back down, the tip recenters itself. This theory is pretty hard to verify since I can't directly observe the tip while it is warm. If I can get the tip centered enough I do see the "maltese cross" figure though sometimes it's a bit off center.
One thing that may affect your lifetime is the filament limit for saturation. I have noticed that if I initially set the cathode to be just saturated, then check it 20-30 minutes later I find I can back the filament drive off by 1 or 2 clicks and it is still saturated. I assume that as the components warm up, the saturation point changes slightly. I do have to tell the students that this is the correct way to operate so that they don't reset the limit themselves. So, I now set my filament limit to be at least one click undersaturated. This way, as it warms up, it will bring itself to the proper saturation point. Note that the Kimball Physics documents indicate that the evaporation rate will triple in going from 1800K to 1850K. This will bring the lifetime from ~2000 hours down to a 600-700 hour range.
If the tip isn't oxidized by one of the students, I can still get close to 2000 hours from a cathode. I did have one student destroy one in 20 minutes by letting the cold trap warm up while it was hot. (I was NOT happy.) Kimball Physics has some useful info in the technical documents on their web site.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: oshel1pe-at-cmich.edu To: colijn.1-at-osu.edu Sent: 4/20/2016 4:01:46 PM
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn?t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can?t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn?t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu} --
==============================Original Headers============================== 6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500 6, 33 -- Received: from cas.cmich.edu ([141.209.15.90]) 6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with
we've had similar issues with the LaB6 filament image (odd shape) and used the gun tilt to max out the screen current as an alignment technique. We also have the filament image moving across the screen when saturation is being approached. But the latter is what I've been told is due to some dirt on the final anode or some nearby electrode perhaps in the accelerator. This wandering is happening with W filaments too and is less critical at lower mags.
I went to put a new LaB6 filament in the other day and it just broke off, much to my embarrassment and I'm putting off buying a new one for a while. FYI our microscope is a JEOL 2000FX and vacuum isn't a problem so long as we replace the ion pump every five years. The new one that broke while I was putting it in was a KP (the Denka are welded and robust).
The thing that has my blood boiling is the so-called long-life FEI gallium sources that don't last more than three months. And as you may know they come in at over $2000 each, as do the expendable extractors similarly priced. The one we got with the new install lasted very nicely, but since then not so much.
Best of luck with your search here, Rob Keyse
On 4/20/2016 4:16 PM, oshel1pe-at-cmich.edu wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu}
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu} --
==============================Original Headers============================== 6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500 6, 33 -- Received: from cas.cmich.edu ([141.209.15.90]) 6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 6, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Wed, 20 Apr 6, 33 -- 2016 16:00:39 -0400 6, 33 -- Message-ID: {5717DFE6.2060309-at-cmich.edu} 6, 33 -- Date: Wed, 20 Apr 2016 16:00:38 -0400 6, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 33 -- MIME-Version: 1.0 6, 33 -- To: micro {microscopy-at-microscopy.com} 6, 33 -- Subject: LaB6 Issues anyone? 6, 33 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-Originating-IP: [141.209.2.100] 6, 33 -- X-Bayes-Prob: 0.9999 (Score 4, tokens from: -at--at-RPTN, default) 6, 33 -- X-Spam-Score: 4.20 (****) [Hold at 6.00] RDNS_NONE,_L_PCM,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.9999:4.0) 6, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 6, 33 -- X-CanItPRO-Stream: default 6, 33 -- X-Canit-Stats-ID: 05QII0DjR - 7690853b811e - 20160420 6, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
JoAnn, In my previous job, our processing workhorse was the Lynx EM tissue processor (the version with the white body and smoke plastic lid). We were quite happy with it, with no problems beyond the occasional user error. We would start with buffer, and proceed through osmium, rinses, graded ETOH, propylene oxide, and then into 3 dilutions of resin. All that was left for us the next day was embedding.
Good luck, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan, MCDB Dept. Ann Arbor, Michigan, USA
On Tue, Apr 19, 2016 at 6:56 PM, {microscopy.listserver-at-gmail.com} wrote:
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Message: Hello listers. I am interested in finding whether there is a processor out there that can take EM samples through dehydration steps.
We will be preparing large tissue blocks (1mm cube) and they need long dehydration steps(30 min each). The current protocol means running up tissue late into the night. A robot would be helpful.
thanks in advance. JoAnn
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Message: Hello listers. I am interested in finding whether there is a processor out there that can take EM samples through dehydration steps.
We will be preparing large tissue blocks (1mm cube) and they need long dehydration steps(30 min each). The current protocol means running up tissue late into the night. A robot would be helpful.
thanks in advance. JoAnn
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Re: (Microscopy) observing a diamond knife in the SEM
Message: Hi Stephane, I'm not sure about putting the diamond knife in the SEM. The epoxy that holds the diamond in place might be compromised. For sure you will have charging from the epoxy.
It might be better to look at the diamond edge under a higher mag in a light microscope. For sure you have either a nick or some hard debris causing your problem. It happens to me a lot because we section drosophila tissue. Try cleaning again with soapy water, rinsing, followed by ethanol. If that doesn't work it might be time to move to the next area or get it resharpened.
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You may be interested in checking out the prices on this web site. I remember Pt far outpacing Au back some years. I suppose Ir was even pricier. Now, Ir comes in at $525/troy ounce, Pt comes in at $981, and Au comes in at $1242 - the most expensive metal in the table.
I suppose that is a supply and demand thing and that many individuals have turned to investing in gold in recent years. Ir and Pt apparently don't rate.
-----Original Message----- X-from: mike_toalson-at-tedpella.com [mailto:mike_toalson-at-tedpella.com] Sent: Monday, April 18, 2016 6:02 PM To: Straszheim, Warren E [BIOTC]
I would agree that a coater capable of laying down a fine layer of Iridium is the least complicated solution, albeit not the cheapest due to target cost. We install a lot of coaters with Iridium targets for this very purpose (Cressington 208HR).
Anyone that would like a copy of an internal study on coating resolutions, please email me directly. You can also have a look at this comparative study:
A comparative study of thin coatings of Au/Pd, Pt and Cr produced by magnetron sputtering for FE-SEM Journal of Microscopy, Vol 189, Pt 1, January 1998, pp 79-89.
Mike Toalson Ted Pella, Inc. PO Box 492477 . Redding, CA 96049 Tel: 530-243-2200 x205 . Cell: 530-356-5921 mike_toalson-at-tedpella.com . www.tedpella.com
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Tuesday, April 12, 2016 1:50 PM To: Mike Toalson
Yes, that is probably right Au/Pd is probably too coarse. We had used gold and the grains were very evident at 50kx. We bought an iridium sputter coater and the grains are small enough that I have never seen them. We often use less than 5 nm of coating thickness.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Tuesday, April 12, 2016 2:44 PM To: Straszheim, Warren E [BIOTC]
Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
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I would agree that a coater capable of laying down a fine layer of Iridium is the least complicated solution, albeit not the cheapest due to target cost. We install a lot of coaters with Iridium targets for this very purpose (Cressington 208HR).
Anyone that would like a copy of an internal study on coating resolutions, please email me directly. You can also have a look at this comparative study:
A comparative study of thin coatings of Au/Pd, Pt and Cr produced by magnetron sputtering for FE-SEM Journal of Microscopy, Vol 189, Pt 1, January 1998, pp 79-89.
Mike Toalson Ted Pella, Inc. PO Box 492477 Redding, CA 96049 Tel: 530-243-2200 x205 Cell: 530-356-5921 mike_toalson-at-tedpella.com www.tedpella.com
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Tuesday, April 12, 2016 1:50 PM To: Mike Toalson
Yes, that is probably right Au/Pd is probably too coarse. We had used gold and the grains were very evident at 50kx. We bought an iridium sputter coater and the grains are small enough that I have never seen them. We often use less than 5 nm of coating thickness.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Tuesday, April 12, 2016 2:44 PM To: Straszheim, Warren E [BIOTC]
Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Tue Apr 12 14:42:13 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3CJgCQf030106 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:42:13 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 3D46F5C8862 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:56 -0500 (CDT) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 4A1D15C885C 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:55 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Topic: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Index: AdGU81PeA6pSzUBqR0GoxnsfL5iGsA== 7, 37 -- Date: Tue, 12 Apr 2016 19:43:37 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE85C21C-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 4e0d9056-73ca-4667-8750-7d6d076dd95f 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 12c18fe0-b9ff-479f-a266-9c4d84d27231 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3CJgCQf030106 ==============================End of - Headers==============================
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I have a 3view, which is basically an ultra-microtome inside an SEM. I don't have conclusive answers, but I can give you my best opinion based on my experience. The short answer is to image the resin rather than the knife in order to see the damage being caused. If you can cut a relatively wide bit of resin then you can coat the block and mount this in the SEM and image the block face. You may see lots of chatter there if you make the face very wide (chatter will look a bit like waves on the block face), but knife marks will come out like perfect straight lines. You'll also see if any seem particularly bad and changes in the amount and type of chatter will give you some information on whether the knife has the same sharpness across the blade. I'd use some empty resin for this sort of test so that you don't have sample density confusing the cutting properties.
To answer the other questions. I do believe that you would crack carbon deposits (particles of resin and, more likely, volatile resin components evaporated during imaging) and stick these onto the knife surface. Whether there would be enough to ruin the knife is another matter as they're likely to be tiny, but its probably safer to avoid imaging the blade directly. Also diamond isn't electrically conductive as far as I know, so you would need to image in a low vacuum mode, or to coat the knife, but I'm not sure if the re-sharpener would like this.
Hope that this helps.
TobyS
Dr Tobias Starborg Senior Experimental Officer: 3DEM Wellcome Centre for Cell Matrix Research Michael Smith Building Manchester M13 9PT Tel: +44(0)1612755170 http://www.polara.manchester.ac.uk
________________________________________ X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com] Sent: 18 April 2016 16:13 To: Tobias Starborg
Hello community!
I have a question about a microtome diamond knife and would be grateful if you would care to share your thoughts with me. The diamond knife (Ultraknife) seems to be damaged or dirty in the middle so that one section is cut in half in the middle. Usually when the blade is damaged I can see some indentation under the binocular at highest mag but in this case I can see nothing, so I tried to clean the blade but it changes nothing. Since we have a SEM, I planned to take a look of the blade in SEM but I wondered if, for any reason, I should avoid that. - Is there a chance that I burn any dirt onto the blade with the electron beam? (I only cut epoxy so it would be some epoxy rests) - Is there a chance that I burn some carbohydrates onto the blade in the same way it occurs in the TEM? - What about charging? I am quite sure that there should be some contact between the diamond blade and the metal boat, so this does not worry me much. - Any other thought? (Apart from the fact that I should avoid to hit the column with the blade :-)) Thanks in advance.
Regards, Stephane
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I have a question about a microtome diamond knife and would be grateful if you would care to share your thoughts with me. The diamond knife (Ultraknife) seems to be damaged or dirty in the middle so that one section is cut in half in the middle. Usually when the blade is damaged I can see some "indentation" under the binocular at highest mag but in this case I can see nothing, so I tried to clean the blade but it changes nothing. Since we have a SEM, I planned to take a look of the blade in SEM but I wondered if, for any reason, I should avoid that. - Is there a chance that I "burn" any dirt onto the blade with the electron beam? (I only cut epoxy so it would be some epoxy rests) - Is there a chance that I "burn" some carbohydrates onto the blade in the same way it occurs in the TEM? - What about charging? I am quite sure that there should be some contact between the diamond blade and the metal boat, so this does not worry me much. - Any other thought? (Apart from the fact that I should avoid to hit the column with the blade :-)) Thanks in advance.
Regards, Stephane
==============================Original Headers============================== 4, 30 -- From nizets2-at-yahoo.com Mon Apr 18 09:55:00 2016 4, 30 -- Received: from nm39.bullet.mail.bf1.yahoo.com (nm39.bullet.mail.bf1.yahoo.com [72.30.239.69]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3IEt0Ki001814 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 18 Apr 2016 09:55:00 -0500 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1460991408; bh=EP6aygMlZTLIPA9ajg9y6HO0MIj6wcBaBMvO/Wa2OUw=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=NK33pYAZOR7n5BxAM5KcHexxMcQg8If2FcXtaUoxOFHNJSuLDQ8cTH7reNL8LX3kqEe51lYG3CCcxw/WD8/WvSrzf/R7HUc2lJH6zAeXLinOP0DWbvYJnusEnz3rIFR2iTTy0Mo2Vx/i/bcnEPnwQ4mWqWCTQhbU+KJ2SkSn2oqWNqB/UTxVUI8typfnR9G/KEXTEyySGmzQ5PIVllDh1wdJmavtw3stDfCeg3uzlpfPssY7ZYSslClzLX3NarZMEtdosXHYH/4ZYdkyO5Or5FUmaPa+swKdfxEsZhYXrnifvYapBmbHJufOu1u7rS2c9xrFicLFD5mv/9jlFmQGtg== 4, 30 -- Received: from [98.139.215.142] by nm39.bullet.mail.bf1.yahoo.com with NNFMP; 18 Apr 2016 14:56:48 -0000 4, 30 -- Received: from [98.139.212.212] by tm13.bullet.mail.bf1.yahoo.com with NNFMP; 18 Apr 2016 14:56:48 -0000 4, 30 -- Received: from [127.0.0.1] by omp1021.mail.bf1.yahoo.com with NNFMP; 18 Apr 2016 14:56:48 -0000 4, 30 -- X-Yahoo-Newman-Property: ymail-3 4, 30 -- X-Yahoo-Newman-Id: 673586.18655.bm-at-omp1021.mail.bf1.yahoo.com 4, 30 -- X-YMail-OSG: AWI1W74VM1lncX0pCshgu1S05cV6PuoqEBCnKJONOLdzPJMoLAtHYYtNeI3vlEx 4, 30 -- mPUI2IA2WvrWIkNhcaIHOwSD3rqeIndg3u8q3taaNDn2sA_rpjbY1dV3yynDEev0XeO0z6KV2D5m 4, 30 -- CixEQOQsZXTRQiTlXYkOEPKG..1CdoxQS2EYr95YBVnyBXmvPHHhtW3IddboK7ruDpKozq3omLpz 4, 30 -- cZgkChVPyaZUUsvQHuv8HJ1PvQzXR2lGFqbdtNiji4bOrMLrgK_bYx4pIY47tKNsxnN9lbF11u4E 4, 30 -- 3AvBx823hRc6v3fUrhpG4mTKF7b5SrTb3Fb75wOPGAY63VjhFUGUa58j1ruWNds2Rs3efiZmbufU 4, 30 -- yeht3YderXSTy6MlESHoiHpShVx3qyQKCxq5GxMHARpViEoY94I7zLQsXflNUAnc8XdHPcJoVI0b 4, 30 -- ynlAShQqvAG1W8dOiqmDt9Yd6_55H2k.FreYL4_ZCLH6VH9Qz0cufX1TWm4cjoaQn8iYBffYlmN5 4, 30 -- aKoZN 4, 30 -- Received: by 76.13.27.54; Mon, 18 Apr 2016 14:56:48 +0000 4, 30 -- Date: Mon, 18 Apr 2016 14:56:47 +0000 (UTC) 4, 30 -- From: Stephane Nizet {nizets2-at-yahoo.com} 4, 30 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 4, 30 -- To: {microscopy-at-microscopy.com} 4, 30 -- Message-ID: {1916337336.2543788.1460991407739.JavaMail.yahoo-at-mail.yahoo.com} 4, 30 -- Subject: observing a diamond knife in SEM 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=UTF-8 4, 30 -- References: {1916337336.2543788.1460991407739.JavaMail.yahoo.ref-at-mail.yahoo.com} 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3IEt0Ki001814 ==============================End of - Headers==============================
Normally, stainless steel should be a fairly straightforward analysis. I would recommend 15-20 kV so you could use the K lines for analysis. Current depends on your EDS settings. You would like to have a decent count rate to shorten the time it takes to get a good spectrum. I like to keep the dead time near but below 30%. What count rate that corresponds to depends on your x-ray detector and your pulse processor settings. On our Oxford X-Max detector, that is about 20 kcps at process time 4. About 0.5 nA gives us that.
I would cautiously let the INCA do auto ID and then confirm the elements. I would expect Fe, Cr, Ni, and maybe some Mn and Si. It depends on the alloy.
If it is possible to remove the piece from the non-conductive tape and transfer it to some carbon tape, I would do that. Hopefully there is not a lot of goo stuck to the chip. If so, you might have to work around it.
EDS in variable pressure mode opens you up to stray signals from the surroundings. The incoming electrons will get scattered and give you a noticeable signal. You would like to be sure that doesn't include any elements that you expect to be present in your sample. You shouldn't be attempting quant on C, so maybe the background holder can be painted with carbon paint. You might try analyses closer to the surroundings and further away to see how the composition changes. This means you will also have trouble trying to get good analyses from adjoining phases if multiple phases are present. You will be able to see which ones are higher and lower in elements, but the absolute levels will be hard to determine.
Also watch out for topography/geometry. EDS normally assumes a flat surface perpendicular to the beam. Tilted surfaces will enhance or detract from the light(er) elements. Watch the background shape and see how it compares to a flat sample known to be stainless steel. The good news for you is that the elements Cr-Ni are of most concern and they are relatively close to each other in energy and of moderate energy that is less subject to changes in absorption. Si is much lighter and more subject to changes.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, April 15, 2016 6:04 PM To: Straszheim, Warren E [BIOTC]
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Email: cross-at-tru.ca Name: Cynthia Ross Friedman
Organization: Thompson Rivers University
Title-Subject: [Filtered] Advice for EDS
Message: Hello -- I admit I am not very skilled with my Oxford Inca EDS yet. Someone has brought me a metal artifact, and would like me to determine if it is stainless steel, and its purity.
Could someone please suggest to me the best parameters to use? Spot size? (in amps) Voltage? Other considertations?
As an added complication, the sample was provided to me on a non-conductive tape, and to get around the charging, I have to use my SEM (a Zeiss LS Evo) at about 55 Pa. I cannot see this being an issue, but do let me know your thoughts.
Thanks in advance! Cindy
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Email: cross-at-tru.ca Name: Cynthia Ross Friedman
Organization: Thompson Rivers University
Title-Subject: [Filtered] Advice for EDS
Message: Hello -- I admit I am not very skilled with my Oxford Inca EDS yet. Someone has brought me a metal artifact, and would like me to determine if it is stainless steel, and its purity.
Could someone please suggest to me the best parameters to use? Spot size? (in amps) Voltage? Other considertations?
As an added complication, the sample was provided to me on a non-conductive tape, and to get around the charging, I have to use my SEM (a Zeiss LS Evo) at about 55 Pa. I cannot see this being an issue, but do let me know your thoughts.
Thanks in advance! Cindy
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We are looking to upgrade one of our spinning disk systems with a new microscope and autofocus system and are looking for comments/reviews of the latest versions from Leica, Zeiss, Nikon and Olympus.
We are familiar with how they function but are looking for comments on how well each works for long term imaging.
Any advice would be greatly appreciated.
Garnet
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Email: james.weston-at-nyu.edu Name: James Weston
Organization: NYU Abu Dhabi
Title-Subject: [Filtered] Life sciences TEM sample prep short courses?
Message: Hello, I am a materials engineer and am soon going to be in charge of a TEM for both materials and biological work. I have no experience in life sciences EM. The TEM manufacturer is providing off-site life science training, however this will not cover sample preparation.
Is there a resource that has listings of sample prep short courses?
Any help/suggestions greatly appreciated. James
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Jisheng, The systems we used at Intel were the VCR IBS, and later, the Gatan PECS (still available). These systems use an argon ion beam striking a target of your choice, and the material sputtered from the target lands on the specimen. The resulting film has an extremely fine grain size. I believe there was a description in one publication of Ron Anderson at IBM using a Gatan duo-mill to do ion beam deposition by putting the target where the TEM specimen should be, and putting the actual specimen ~1 cm away with a custom made fixture.
John Mardinly, Retired from ASU
On Apr 13, 2016, at 10:49 PM, Ji Sheng Ma {jisheng.ma-at-monash.edu} wrote:
Dear John,
I've got your email from the emal-list. I am very interested what you mentioned that "to get extremely fine grain size is to use ion-beam deposition".
I am wondering the ion-beam deposition is referring focused ion beam? If yes, it could be extremely useful. I thought (and I also tried) it was very hard to achieve very thin coating (usually the Pt coating with a lot of carbon) in a FIB microscope even use electron beam coating at a very low coating rate.
Maybe I misunderstood you. Otherwise please let me know if you think it is also possible to achieve good coating using FIB in a FIB microscope and your suggestion on coating procedures to do this.
Thanks for your time.
Kind Regards,
Jisheng
On 14 April 2016 at 15:22, Jisheng Ma {jisheng.ma-at-gmail.com} wrote: ---------- Forwarded message ----------
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My experience with chromium is that it seems to oxidize during deposition, such that freshly deposited films that are covered with a cap that blocks exposure to air are up to half oxygen when analyzed. Nevertheless, under HRTEM, they appear to have grain size so much smaller than any TEM specimen thickness that the grains are not visible. Another way to get extremely fine grain size is to use ion-beam deposition rather than sputtering or evaporation. Osmium, whatever it's merits from a grain size consideration, is hazardous, so you would need to implement a safety program around it, which could make it more trouble than it is worth.
John Mardinly, Retired from ASU
Hi Tom
I would guess that you are going to be working at a resolution that will exceed the grain size of Au/Pd. While there are certainly exceptions, I typically suggest the following as a very rough guideline:
Gold up to ~10kx mag Gold/Palladium up to ~50kx mag Platinum up to ~100-150kx mag Above 150kx mag, either Chromium, Iridium, or Osmium. Each has its own advantages and drawbacks:
Chromium is probably the cheapest option, but oxidizes very quickly into a non-conductive film. Iridium targets can be very expensive, but are available for most coaters. Osmium coating is done in a dedicated coater that will not sputter other materials, but the film is inert and amorphous. The source material is relatively cheap, but the coaters are an investment.
A really good source of information is "Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis" by Patrick Echlin, who used to teach at the Lehigh Microscopy School.
I'd be happy to continue this offline if you have more questions, or are looking for more detailed information.
Best regards,
Jeff
Jeffrey A. Hall | Microscopist SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
-- Dr. Jisheng Ma FIB/SEM Microscopist and Ultramicrotomist
Monash Centre for Electron Microscopy Monash University Room G29, Building 81, Clayton Campus 10 Innovation Walk Clayton VIC 3800 Australia
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Email: blayjorge-at-gmail.com Name: Jorge A Santiago-Blay
Organization: NMNH
Title-Subject: [Filtered] Removing exudates from insect wings
Message: We have imaged beautiful structures on the surface of insect wings. Yet, we want to try to eliminate the possibility some of the structures" are *not* some sort of exudate (e.g. cuticular hydrocarbon, etc.) from the wings. I am aware that pentane, hexane can get the job done if the materials are suspect to be thin layers but what is the stuff is thinker? Would hexamethyldisilane (HMDS, in a hood) work?
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----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 13.04.16 um 15:32 schrieb stefan.diller-at-t-online.de: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear All, anybody out there who can send me a PDF copy of the user manual of the Ultra-Trim?
Thanks, Stefan
==============================Original Headers============================== 6, 24 -- From stefan.diller-at-t-online.de Thu Apr 14 03:25:56 2016 6, 24 -- Received: from mailout10.t-online.de (mailout10.t-online.de [194.25.134.21]) 6, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3E8Pt8B030507 6, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 Apr 2016 03:25:55 -0500 6, 24 -- Received: from fwd06.aul.t-online.de (fwd06.aul.t-online.de [172.20.26.150]) 6, 24 -- by mailout10.t-online.de (Postfix) with SMTP id EAE4B2D1831 6, 24 -- for {microscopy-at-microscopy.com} ; Thu, 14 Apr 2016 10:27:27 +0200 (CEST) 6, 24 -- Received: from mac-pro.fritz.box (Jl6e6cZDrhHNCHuJUiTiQa7xS8KMp4WIK9aehd4Lxw+unrKcTbFDOkCUcdXCrojwXi-at-[93.203.68.88]) by fwd06.t-online.de 6, 24 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 6, 24 -- esmtp id 1aqccU-3MWINc0; Thu, 14 Apr 2016 10:27:22 +0200 6, 24 -- Subject: Re: [Microscopy] LEICA / Reichert Ultra-Trim manual - solved 6, 24 -- References: {201604131332.u3DDWrsL020137-at-ns.microscopy.com} 6, 24 -- To: microscopy-at-microscopy.com 6, 24 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 24 -- Message-ID: {570F546A.50509-at-t-online.de} 6, 24 -- Date: Thu, 14 Apr 2016 10:27:22 +0200 6, 24 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 6, 24 -- Gecko/20100101 Thunderbird/38.7.2 6, 24 -- MIME-Version: 1.0 6, 24 -- In-Reply-To: {201604131332.u3DDWrsL020137-at-ns.microscopy.com} 6, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 24 -- Content-Transfer-Encoding: 7bit 6, 24 -- X-ID: Jl6e6cZDrhHNCHuJUiTiQa7xS8KMp4WIK9aehd4Lxw+unrKcTbFDOkCUcdXCrojwXi 6, 24 -- X-TOI-MSGID: ff2465a1-7b35-431a-8505-628a969584e0 ==============================End of - Headers==============================
As we bought our first FE-SEM, we asked ourself the same question, and finally bought... nothing. Our FE-SEM is now more then 15 years old, and in 95% of the cases, playing with accellerating voltage, beam current, detectors and scan speed, it's not necessary to coat. Our new FE-SEM is now not far from the door, but without any coater again !
In the mean time I build a multi purpouse evaporator fitted among others with a magnetron source. I bought then a Ir target, to be able to try and in case it's necessary... I use it from time to time for fabric samples, because I'm too lazy to follow the drifting of the low energy condtions for these samples. But they need only low mag and could be observed on a good W type SEM with the classic 20 nm grain size gold coating from our 30 years old Balzers.
The only coating which remains necessary on FE-SEM is carbon for EDX.
Of coarse, as we work in material science (but often on oxydes) and not in biology, our samples are probably different from yours.
2 cts !
Jacques
Le 12/04/2016 22:02, tbargar-at-unmc.edu a ecrit : ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Tue Apr 12 14:42:13 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3CJgCQf030106 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:42:13 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 3D46F5C8862 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:56 -0500 (CDT) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 4A1D15C885C 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:55 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Topic: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Index: AdGU81PeA6pSzUBqR0GoxnsfL5iGsA== 7, 37 -- Date: Tue, 12 Apr 2016 19:43:37 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE85C21C-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 4e0d9056-73ca-4667-8750-7d6d076dd95f 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 12c18fe0-b9ff-479f-a266-9c4d84d27231 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3CJgCQf030106 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Electron Microscopy (EM) Workshop and Maryland NanoDay 2016 Conference
Message: Dear EM and FIB Friends:
I would like to take this opportunity to invite you to the Electron Microscopy (EM) Workshop and Maryland NanoDay 2016 Conference that will be held at the University of Maryland, College Park, Maryland on May 17 and 18, 2016.
Below are some of the highlights for this meeting:
* The theme of EM Workshop: Recent Advances in Electron and Ion Microscopy (EM Workshop Day, May 17). The EM Workshop covers a broad spectrum of newest development in electron microscopy and focus ion beam and related technology.
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Additional information and registration can be found at http://www.nanocenter.umd.edu
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My experience with chromium is that it seems to oxidize during deposition, such that freshly deposited films that are covered with a cap that blocks exposure to air are up to half oxygen when analyzed. Nevertheless, under HRTEM, they appear to have grain size so much smaller than any TEM specimen thickness that the grains are not visible. Another way to get extremely fine grain size is to use ion-beam deposition rather than sputtering or evaporation. Osmium, whatever it's merits from a grain size consideration, is hazardous, so you would need to implement a safety program around it, which could make it more trouble than it is worth.
John Mardinly, Retired from ASU
Hi Tom
I would guess that you are going to be working at a resolution that will exceed the grain size of Au/Pd. While there are certainly exceptions, I typically suggest the following as a very rough guideline:
Gold up to ~10kx mag Gold/Palladium up to ~50kx mag Platinum up to ~100-150kx mag Above 150kx mag, either Chromium, Iridium, or Osmium. Each has its own advantages and drawbacks:
Chromium is probably the cheapest option, but oxidizes very quickly into a non-conductive film. Iridium targets can be very expensive, but are available for most coaters. Osmium coating is done in a dedicated coater that will not sputter other materials, but the film is inert and amorphous. The source material is relatively cheap, but the coaters are an investment.
A really good source of information is "Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis" by Patrick Echlin, who used to teach at the Lehigh Microscopy School.
I'd be happy to continue this offline if you have more questions, or are looking for more detailed information.
Best regards,
Jeff
Jeffrey A. Hall | Microscopist SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Tuesday, April 12, 2016 4:01 PM To: Jeff Hall {jhall-at-2spi.com}
Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Tue Apr 12 14:42:13 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3CJgCQf030106 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:42:13 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 3D46F5C8862 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:56 -0500 (CDT) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 4A1D15C885C 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:55 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Topic: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Index: AdGU81PeA6pSzUBqR0GoxnsfL5iGsA== 7, 37 -- Date: Tue, 12 Apr 2016 19:43:37 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE85C21C-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 4e0d9056-73ca-4667-8750-7d6d076dd95f 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 12c18fe0-b9ff-479f-a266-9c4d84d27231 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3CJgCQf030106 ==============================End of - Headers==============================
We need a new cryomicrotome for 'thick' sectioning for LM transmission imaging. We cut rubber and plastic "thick" sections (c.1 micron thick). We now use a Leica RM2165 cryomicrotome with the LN21 cryo unit to do this. The LN21 cools the specimen to -150 degrees C. Our equipment is getting old and we need a replacement very soon. The RM2265 can still be purchased from Leica. However, apparently, the LN22 cryo unit that fits the RM2265 has been discontinued. As for cryostats: the minimum temperatures are too high (typically -50 degrees C). Therefore, we cannot use a typical cryostat (such as for biological tissue etc.) We have searched the internet and been unsuccessful in finding information.
Can anyone direct us to a solution 'similar' to the RM2265/LN22 from any USA supplier? We prefer new equipment as opposed to used. Thanks in advance for your advice.
Dear All, anybody out there who can send me a PDF copy of the user manual of the Ultra-Trim?
Thanks, Stefan
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----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: mel-at-rms.org.uk Name: Mel Reedman
Organization: Royal Microscopical Society
Title-Subject: [Filtered] RMS Medal Series Nominations
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The RMS Medal Series, awards scientific achievements in each of the Science Sections as well as awarding the unsung heroes and those who volunteer a huge amount of their time and energy to the RMS, helping the next generation of microscopists.
The RMS are inviting nominations for the following Medals until 30 April 2016.
- President's Medal for Services to the Society for an exceptional voluntary contribution to the work of the RMS. - Vice-Presidents' Medal for Microscopy Research and Laboratory Support recognises the unsung heroes of microscopy by making an award to an engineer, technician or laboratory research support scientist. - Alan Agar Medal for Electron Microscopy outstanding scientific achievements applying electron microscopy in the field of physical or life sciences. - Medal for Light Microscopy outstanding scientific achievements applying or developing new forms of light microscopy. - Medal for Life Sciences outstanding scientific achievements applying microscopy in the field of cell biology. - Medal for Innovation in Applied Microscopy for Materials Science outstanding scientific achievements in applying microscopy in the field of materials science. - Medal for Scanning Probe Microscopy outstanding progress made in the field of scanning probe microscopy (SPM.) - The Pearse Prize a scientist who has made a valuable contribution either to the development of a new technique or through the application of existing methods.
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Hello Tom, It depends mainly on your samples and resolution you want to get. However, for FEG you should use a high resolution sputter coater. I have good experiences with platinum coating (~ 3 nm) for biological samples. For x-ray microanalysis we use 10 nm of silver coating (biological samples). In some cases we do not coat the samples at all. But all depends on detectors you have on your FEG and the mode of FEG SEM you use.
Best regards
Oldrich
-- Old ich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vide ska 1083 142 20 Prague 4 Czech Republic
On Tue, 12 Apr 2016 14:47:04 -0500, tbargar-at-unmc.edu wrote :
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Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Tue Apr 12 14:42:13 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3CJgCQf030106 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:42:13 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 3D46F5C8862 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:56 -0500 (CDT) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 4A1D15C885C 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:55 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 --
Hi Tom
I would guess that you are going to be working at a resolution that will exceed the grain size of Au/Pd. While there are certainly exceptions, I typically suggest the following as a very rough guideline:
Gold up to ~10kx mag Gold/Palladium up to ~50kx mag Platinum up to ~100-150kx mag Above 150kx mag, either Chromium, Iridium, or Osmium. Each has its own advantages and drawbacks:
Chromium is probably the cheapest option, but oxidizes very quickly into a non-conductive film. Iridium targets can be very expensive, but are available for most coaters. Osmium coating is done in a dedicated coater that will not sputter other materials, but the film is inert and amorphous. The source material is relatively cheap, but the coaters are an investment.
A really good source of information is "Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis" by Patrick Echlin, who used to teach at the Lehigh Microscopy School.
I'd be happy to continue this offline if you have more questions, or are looking for more detailed information.
Best regards,
Jeff
Jeffrey A. Hall | Microscopist SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Tuesday, April 12, 2016 4:01 PM To: Jeff Hall {jhall-at-2spi.com}
Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Tue Apr 12 14:42:13 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3CJgCQf030106 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:42:13 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 3D46F5C8862 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:56 -0500 (CDT) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 4A1D15C885C 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:55 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Topic: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Index: AdGU81PeA6pSzUBqR0GoxnsfL5iGsA== 7, 37 -- Date: Tue, 12 Apr 2016 19:43:37 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE85C21C-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 4e0d9056-73ca-4667-8750-7d6d076dd95f 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 12c18fe0-b9ff-479f-a266-9c4d84d27231 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3CJgCQf030106 ==============================End of - Headers==============================
Yes, that is probably right Au/Pd is probably too coarse. We had used gold and the grains were very evident at 50kx. We bought an iridium sputter coater and the grains are small enough that I have never seen them. We often use less than 5 nm of coating thickness.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Tuesday, April 12, 2016 2:44 PM To: Straszheim, Warren E [BIOTC]
Dear Listers,
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Tue Apr 12 14:42:13 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3CJgCQf030106 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:42:13 -0500 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id 3D46F5C8862 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:56 -0500 (CDT) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 4A1D15C885C 7, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Apr 2016 14:17:55 -0500 (CDT) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Topic: Type of sputter coating needed for Field Emission SEM 7, 37 -- Thread-Index: AdGU81PeA6pSzUBqR0GoxnsfL5iGsA== 7, 37 -- Date: Tue, 12 Apr 2016 19:43:37 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE85C21C-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 4e0d9056-73ca-4667-8750-7d6d076dd95f 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 12c18fe0-b9ff-479f-a266-9c4d84d27231 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3CJgCQf030106 ==============================End of - Headers==============================
What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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I have a need to add the Z6040 to HM20 resin to be cured with UV -at- -45C. I ve been told that Z6040 can be used with HM20 however, I did a test block and the resin never cured.
Has anyone used Z6040 with HM20? Did you have to change the amount of accelerator?
I use Z6040 Primer (EMS-50440-10) with Epoxy resins and LRW heat and cold cured. The primer always works with these resins and I haven t needed to change the recipe.
Karen
==============================Original Headers============================== 9, 43 -- From vau-at-ufl.edu Mon Apr 11 10:38:48 2016 9, 43 -- Received: from smtp.ufl.edu (smtp-prod03.osg.ufl.edu [128.227.74.219]) 9, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3BFcmuJ027228 9, 43 -- for {Microscopy-at-Microscopy.com} ; Mon, 11 Apr 2016 10:38:48 -0500 9, 43 -- X-UFL-GatorLink-Authenticated: authenticated as () with from 10.36.133.38 9, 43 -- Received: from exmbxprd12.ad.ufl.edu ([10.36.133.38]) 9, 43 -- by smtp.ufl.edu (8.14.4/8.14.4/3.0.0) with ESMTP id u3BFe4iE042874 9, 43 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 9, 43 -- for {Microscopy-at-Microscopy.com} ; Mon, 11 Apr 2016 11:40:10 -0400 9, 43 -- Received: from exmbxprd14.ad.ufl.edu (10.36.133.40) by exmbxprd12.ad.ufl.edu 9, 43 -- (10.36.133.38) with Microsoft SMTP Server (TLS) id 15.0.1156.6; Mon, 11 Apr 9, 43 -- 2016 11:40:07 -0400 9, 43 -- Received: from exmbxprd14.ad.ufl.edu ([fe80::82f:a9f2:902a:81cc]) by 9, 43 -- exmbxprd14.ad.ufl.edu ([fe80::82f:a9f2:902a:81cc%19]) with mapi id 9, 43 -- 15.00.1156.000; Mon, 11 Apr 2016 11:40:07 -0400 9, 43 -- From: "Kelley,Karen L" {vau-at-ufl.edu} 9, 43 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 9, 43 -- Subject: Z6040 with HM20 9, 43 -- Thread-Topic: Z6040 with HM20 9, 43 -- Thread-Index: AQHRlAhr6/5T7u9VdESfljoNfP2LLA== 9, 43 -- Date: Mon, 11 Apr 2016 15:40:06 +0000 9, 43 -- Message-ID: {D3313D95.1FF18%vau-at-ufl.edu} 9, 43 -- Accept-Language: en-US 9, 43 -- Content-Language: en-US 9, 43 -- X-MS-Has-Attach: 9, 43 -- X-MS-TNEF-Correlator: 9, 43 -- user-agent: Microsoft-MacOutlook/14.5.8.151023 9, 43 -- x-ms-exchange-messagesentrepresentingtype: 1 9, 43 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 43 -- x-originating-ip: [10.36.134.14] 9, 43 -- Content-Type: text/plain; charset="iso-8859-1" 9, 43 -- Content-ID: {EB0260EB26E5A34CA92CA6077C190E24-at-ad.ufl.edu} 9, 43 -- MIME-Version: 1.0 9, 43 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2016-04-11_11:,, 9, 43 -- signatures=0 9, 43 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 9, 43 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 9, 43 -- adjust=0 reason=mlx scancount=1 engine=8.0.1-1603180000 9, 43 -- definitions=main-1604110228 9, 43 -- X-Spam-Level: * 9, 43 -- X-UFL-Spam-Level: * 9, 43 -- Content-Transfer-Encoding: 8bit 9, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3BFcmuJ027228 ==============================End of - Headers==============================
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Email: gary-at-microtechnics.com Name: Dr. Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] CPD system suggestions
Message: Greetings listers. I'm seeking feedback about CPD units for dehydration of bio specimens for SEM. I'd like an automated system that does more push button GO than manual knob manipulation. Any suggestions for this type of unit? For CO2, what general size cylinders are useful for occasional drying?
All inputs are appreciated, TIA. gary g.
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Usually the screens get covered, more toward the center, with carbon contamination, which makes them darker. Occasionally they will get tiny burns by someone doing microbeam analysis or diffraction. There a few companies that re-coat them (for a MUCH lower price than the OEM manufacturer) that can be found by searching "fluorescent screen re-coating" in Google. Even Amazon.com.uk lists a service!
John Mardinly, Retired from ASU
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Message: I would like to hear from microscopy listserver regarding the transmission electron microscope florescent screen service which has some dirt particles accumulated over a period of years likely to be the traces of carbon film /sample charging effect of nano materials and etc. Thanks in advanec
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Thanks,
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PSM's April meeting will be held on Thursday the 21st of April at Villanova University at 6pm. There will be a short business meeting (with pizza) followed by a talk by Linden Gledhill.
Here is some information on Linden and the talk. The presentation "Creating Commercial Art from Microscopy" will focus on the creative process and provide insight through discussion of specific projects for commercial marketing. Linden Gledhill is an artist who explores the physical world at different image scales and fragments of time. His education in science has led to the use of advanced microscopy and high speed equipment to create unexpected imagery revealing the physical beauty which surrounds us.
A passion for photography has led to numerous collaborations with other artists and designers resulting in promotional art work and effective advertisement campaigns. His work can be found in a wide range of products including books, snow boards, record sleeves, promotional video and apparel. Corporate clients included ExxonMobil, GMC, Canon,British Telecom and musicians such as Ryan Teague and Jon Hopkins. In addition, an extensive selection of archive images are available for licensing and printing and can be viewed at Flickr.com
commercial web site http://www.lindengledhill.com/
Linden has a Ph.D in biochemistry from the University of Nottingham (UK) and is the VP of Medicine and Process Delivery (MPD) at Glaxosmithkline based in Philadelphia. MPD team members lead multidisciplinary projects teams for the development of biopharmaceutical products for a wide variety of diseases such as cancer, diabetes and rheumatoid arthritis.
Please see our Facebook page for further meeting details https://www.facebook.com/PhiladelphiaSocietyforMicroscopy/
==============================Original Headers============================== 9, 34 -- From carltra-at-aol.com Thu Apr 7 12:56:31 2016 9, 34 -- Received: from omr-m011e.mx.aol.com (omr-m011e.mx.aol.com [204.29.186.11]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u37HuVCZ006061 9, 34 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Apr 2016 12:56:31 -0500 9, 34 -- Received: from mtaout-aag01.mx.aol.com (mtaout-aag01.mx.aol.com [172.26.126.77]) 9, 34 -- by omr-m011e.mx.aol.com (Outbound Mail Relay) with ESMTP id 263F13800060 9, 34 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Apr 2016 13:57:37 -0400 (EDT) 9, 34 -- Received: from RobertPC (c-73-165-193-128.hsd1.pa.comcast.net [73.165.193.128]) 9, 34 -- (using TLSv1 with cipher AES256-SHA (256/256 bits)) 9, 34 -- (No client certificate requested) 9, 34 -- by mtaout-aag01.mx.aol.com (MUA/Third Party Client Interface) with ESMTPSA id CD387380000A9 9, 34 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Apr 2016 13:57:36 -0400 (EDT) 9, 34 -- From: "Robert Carlton" {carltra-at-aol.com} 9, 34 -- To: {Microscopy-at-Microscopy.Com} 9, 34 -- Subject: Philadelphia Society For Microscopy Meeting - Thursday April 21st Villanova University 9, 34 -- Date: Thu, 7 Apr 2016 13:57:36 -0400 9, 34 -- Message-ID: {001601d190f6$f8c83540$ea589fc0$-at-aol.com} 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; 9, 34 -- charset="us-ascii" 9, 34 -- Content-Transfer-Encoding: 7bit 9, 34 -- X-Mailer: Microsoft Outlook 16.0 9, 34 -- Thread-Index: AdGQ9sY9xSIEUHB2RUW0+p1t4ZNkpw== 9, 34 -- Content-Language: en-us 9, 34 -- x-aol-global-disposition: G 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=mx.aol.com; 9, 34 -- s=20150623; t=1460051857; 9, 34 -- bh=7JqvkOOBCnSmDQeEoriO+WKlP0Wddb+GnSjIbQO2Lfs=; 9, 34 -- h=From:To:Subject:Message-ID:Date:MIME-Version:Content-Type; 9, 34 -- b=J6YSsrr054Mg+tUA/WU0WhUom6HICseed6g7wLt27LwuVWrwPgW/tIEwbYPWE9bHW 9, 34 -- y4ehep3A/xo804jhRO2gMh1gILNJQXMhhduWfnS9a5rfhdcI8ir2sBfSHTiyEBcUkJ 9, 34 -- c2FPUK2n5n0yfGnmClVI8Mln1OV6t1YvZPDaH++g= 9, 34 -- x-aol-sid: 3039ac1a7e4d57069f900427 9, 34 -- X-AOL-IP: 73.165.193.128 ==============================End of - Headers==============================
Our Tecnai G20 has past the 10 years flag and I am wondering which moving piece we need to care of/service. In the microscope itself there are only few moving pieces, some of which we almost never move (apertures) but an important one is the goniometer and it is moving all the time. I still have no problem with it but I would like to prevent them instead of having to cure them later so I would like to know what is advised, what is your feedback with goniometers? Do they need any service at all or are they good to go for the lifetime of the system? Of less concern is the trackball to move the goniometer, but still I wonder if I need to clean any part inside before it stops working? It is a matter of fact that the air is filtered and very clean so the material does not take a lot of dust even after 10 years but still I would like to know.
Many thanks in advance for the answers and no thank you at all for the flow of automatic replies.
Regards Stephane
-------------------------------------------- On Wed, 4/6/16, jkrupp-at-deltacollege.edu {jkrupp-at-deltacollege.edu} wrote:
Does anyone have advice about lubricating lab instruments?
We often have controls, gears, sliding blocks, shafts that seem to need a little help. Nothing like a big overhaul or major service, just a little tightness.
I know about the need for special lubricants in specific places, but do you think it would be OK to try to loosen up some things by applying some lubricant to specific places?
Have you found anything that seems to work well and is compatible with scientific applications?
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 38 -- From prvs=18974ac4cd=jkrupp-at-deltacollege.edu Wed Apr 6 10:47:50 2016 9, 38 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u36Fln73031703 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 10:47:50 -0500 9, 38 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 9, 38 -- by localhost (Email Security Appliance) with SMTP id 5DC2A178287_7052FE5B 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 15:48:53 +0000 (GMT) 9, 38 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 9, 38 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTP id 4BEEA17B685_7052FE5F 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 15:48:53 +0000 (GMT) 9, 38 -- Received: from localhost (localhost [127.0.0.1]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 46869F80DF41 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:53 -0700 (PDT) 9, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 9, 38 -- with ESMTP id sMQCXOYTrSsh for {Microscopy-at-microscopy.com} ; 9, 38 -- Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- Received: from localhost (localhost [127.0.0.1]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 60B66F7FABE1 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 9, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 9, 38 -- with ESMTP id 8_1GmB7_X2BL for {Microscopy-at-microscopy.com} ; 9, 38 -- Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- Received: from [10.246.128.227] (unknown [10.246.128.227]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 436A6F7FABDE 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 9, 38 -- Content-Type: text/plain; charset=us-ascii 9, 38 -- Subject: Lubricating instruments 9, 38 -- Message-Id: {C34C98A5-D038-4CA5-B429-E6CFDA32DFD4-at-deltacollege.edu} 9, 38 -- Date: Wed, 6 Apr 2016 08:48:46 -0700 9, 38 -- To: Microscopy-at-microscopy.com 9, 38 -- Mime-Version: 1.0 (Mac OS X Mail 9.2 \(3112\)) 9, 38 -- X-Mailer: Apple Mail (2.3112) 9, 38 -- Content-Transfer-Encoding: 8bit 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u36Fln73031703 ==============================End of - Headers==============================
I am starting the adventure of restoring an old and dilapidated Hitachi S-415A SEM (for hobbyist use).
Amongst major work, E-T detector coatings have been practically removed, power supply is damaged (someone apparently decided to use an angle grinder on it) and lots of mechanical misc work all over.
Does anybody have a set of schematic diagrams for the S-415A electronics that could send to me ?
Any help will be greatly appreciated!
-- Sebastian Diaz Houston, TX mobile: 832-470-0386
I've found a small amount of white lithium grease works for sliding surfaces of focusing racks, moving blocks in microscope heads for picking camera/eye, things like that. Even used a bit of Braycote (a true high-vacuum grease) to stop the EDS on our STEM from screaming as it inserted/retracted. A *tiny* bit - stuff is hideously expensive.
Phil
Does anyone have advice about lubricating lab instruments?
We often have controls, gears, sliding blocks, shafts that seem to need a little help. Nothing like a big overhaul or major service, just a little tightness.
I know about the need for special lubricants in specific places, but do you think it would be OK to try to loosen up some things by applying some lubricant to specific places?
Have you found anything that seems to work well and is compatible with scientific applications?
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Wed Apr 6 11:31:55 2016 4, 32 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u36GVtIg024545 4, 32 -- for {microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 11:31:55 -0500 4, 32 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 32 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u36GWtK8009516; 4, 32 -- Wed, 6 Apr 2016 12:32:55 -0400 4, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 4, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.248.2; Wed, 6 Apr 2016 12:32:55 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 4, 32 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Wed, 6 Apr 4, 32 -- 2016 12:32:55 -0400 4, 32 -- Message-ID: {57053A36.8040302-at-cmich.edu} 4, 32 -- Date: Wed, 6 Apr 2016 12:32:54 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: {jkrupp-at-deltacollege.edu} , micro {microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] Lubricating instruments 4, 32 -- References: {201604061609.u36G94sQ015982-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201604061609.u36G94sQ015982-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: -1.60 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 05QD4wTKa - 2087d27b582d - 20160406 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Hi Jon, We used molybdenum disulfide paste for such jobs in our surface science lab. No vapors and no spreading, like oils do. Also works for lubricating nuts and bolts that might seize after equipment baking. Comes in little tubes.
Regards, Larry Scipioni
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Wednesday, April 06, 2016 12:08 PM To: LES-at-ZSGENETICS.COM
Does anyone have advice about lubricating lab instruments?
We often have controls, gears, sliding blocks, shafts that seem to need a little help. Nothing like a big overhaul or major service, just a little tightness.
I know about the need for special lubricants in specific places, but do you think it would be OK to try to loosen up some things by applying some lubricant to specific places?
Have you found anything that seems to work well and is compatible with scientific applications?
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 38 -- From prvs=18974ac4cd=jkrupp-at-deltacollege.edu Wed Apr 6 10:47:50 2016 9, 38 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u36Fln73031703 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 10:47:50 -0500 9, 38 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 9, 38 -- by localhost (Email Security Appliance) with SMTP id 5DC2A178287_7052FE5B 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 15:48:53 +0000 (GMT) 9, 38 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 9, 38 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTP id 4BEEA17B685_7052FE5F 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 15:48:53 +0000 (GMT) 9, 38 -- Received: from localhost (localhost [127.0.0.1]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 46869F80DF41 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:53 -0700 (PDT) 9, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 9, 38 -- with ESMTP id sMQCXOYTrSsh for {Microscopy-at-microscopy.com} ; 9, 38 -- Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- Received: from localhost (localhost [127.0.0.1]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 60B66F7FABE1 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 9, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 9, 38 -- with ESMTP id 8_1GmB7_X2BL for {Microscopy-at-microscopy.com} ; 9, 38 -- Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- Received: from [10.246.128.227] (unknown [10.246.128.227]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 436A6F7FABDE 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 9, 38 -- Content-Type: text/plain; charset=us-ascii 9, 38 -- Subject: Lubricating instruments 9, 38 -- Message-Id: {C34C98A5-D038-4CA5-B429-E6CFDA32DFD4-at-deltacollege.edu} 9, 38 -- Date: Wed, 6 Apr 2016 08:48:46 -0700 9, 38 -- To: Microscopy-at-microscopy.com 9, 38 -- Mime-Version: 1.0 (Mac OS X Mail 9.2 \(3112\)) 9, 38 -- X-Mailer: Apple Mail (2.3112) 9, 38 -- Content-Transfer-Encoding: 8bit 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u36Fln73031703 ==============================End of - Headers==============================
Does anyone have advice about lubricating lab instruments?
We often have controls, gears, sliding blocks, shafts that seem to need a little help. Nothing like a big overhaul or major service, just a little tightness.
I know about the need for special lubricants in specific places, but do you think it would be OK to try to loosen up some things by applying some lubricant to specific places?
Have you found anything that seems to work well and is compatible with scientific applications?
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 9, 38 -- From prvs=18974ac4cd=jkrupp-at-deltacollege.edu Wed Apr 6 10:47:50 2016 9, 38 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u36Fln73031703 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 10:47:50 -0500 9, 38 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 9, 38 -- by localhost (Email Security Appliance) with SMTP id 5DC2A178287_7052FE5B 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 15:48:53 +0000 (GMT) 9, 38 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 9, 38 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTP id 4BEEA17B685_7052FE5F 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 15:48:53 +0000 (GMT) 9, 38 -- Received: from localhost (localhost [127.0.0.1]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 46869F80DF41 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:53 -0700 (PDT) 9, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 9, 38 -- with ESMTP id sMQCXOYTrSsh for {Microscopy-at-microscopy.com} ; 9, 38 -- Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- Received: from localhost (localhost [127.0.0.1]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 60B66F7FABE1 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 9, 38 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 9, 38 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 9, 38 -- with ESMTP id 8_1GmB7_X2BL for {Microscopy-at-microscopy.com} ; 9, 38 -- Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- Received: from [10.246.128.227] (unknown [10.246.128.227]) 9, 38 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 436A6F7FABDE 9, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:48:52 -0700 (PDT) 9, 38 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 9, 38 -- Content-Type: text/plain; charset=us-ascii 9, 38 -- Subject: Lubricating instruments 9, 38 -- Message-Id: {C34C98A5-D038-4CA5-B429-E6CFDA32DFD4-at-deltacollege.edu} 9, 38 -- Date: Wed, 6 Apr 2016 08:48:46 -0700 9, 38 -- To: Microscopy-at-microscopy.com 9, 38 -- Mime-Version: 1.0 (Mac OS X Mail 9.2 \(3112\)) 9, 38 -- X-Mailer: Apple Mail (2.3112) 9, 38 -- Content-Transfer-Encoding: 8bit 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u36Fln73031703 ==============================End of - Headers==============================
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course May 17-19, 2016.
In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further TEM training details and registration information, please follow the link below: https://www.mccrone.com/transmission-electron-microscopy-tem-course
Chris Gorman P. 630.887.7100
==============================Original Headers============================== 8, 42 -- From CGorman-at-hookecollege.com Wed Apr 6 08:18:38 2016 8, 42 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u36DIc2H006420 8, 42 -- for {microscopy-at-microscopy.com} ; Wed, 6 Apr 2016 08:18:38 -0500 8, 42 -- X-ASG-Debug-ID: 1459948781-07bbd410f72fb970001-4CH8be 8, 42 -- Received: from TMGEX2.tmg.mccrone.com ([192.168.101.54]) by spam.mccrone.com with ESMTP id PlWikQkipHHAteE4 for {microscopy-at-microscopy.com} ; Wed, 06 Apr 2016 08:19:41 -0500 (CDT) 8, 42 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com 8, 42 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by 8, 42 -- TMGEX2.tmg.mccrone.com (192.168.101.74) with Microsoft SMTP Server (TLS) id 8, 42 -- 15.0.913.22; Wed, 6 Apr 2016 08:19:40 -0500 8, 42 -- Received: from TMGEX1.tmg.mccrone.com ([::1]) by TMGEX1.tmg.mccrone.com 8, 42 -- ([fe80::3c35:1cb2:6b8a:e82d%14]) with mapi id 15.00.0913.011; Wed, 6 Apr 2016 8, 42 -- 08:19:40 -0500 8, 42 -- From: Christine Gorman {CGorman-at-hookecollege.com} 8, 42 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 8, 42 -- Subject: Transmission Electron Microscopy short course May 17-19, 2016 8, 42 -- Thread-Topic: Transmission Electron Microscopy short course May 17-19, 2016 8, 42 -- X-ASG-Orig-Subj: Transmission Electron Microscopy short course May 17-19, 2016 8, 42 -- Thread-Index: AdGQBuNMuJtGGvXaTGKIsO5I+XpzyA== 8, 42 -- Date: Wed, 6 Apr 2016 13:19:39 +0000 8, 42 -- Message-ID: {ca90847227e04efcb0490c0b1196869c-at-TMGEX1.tmg.mccrone.com} 8, 42 -- Accept-Language: en-US 8, 42 -- Content-Language: en-US 8, 42 -- X-MS-Has-Attach: 8, 42 -- X-MS-TNEF-Correlator: 8, 42 -- x-originating-ip: [192.168.101.154] 8, 42 -- Content-Type: text/plain; charset="iso-8859-1" 8, 42 -- MIME-Version: 1.0 8, 42 -- X-Barracuda-Connect: UNKNOWN[192.168.101.54] 8, 42 -- X-Barracuda-Start-Time: 1459948781 8, 42 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi 8, 42 -- X-Barracuda-Scan-Msg-Size: 463 8, 42 -- X-Virus-Scanned: by bsmtpd at mccrone.com 8, 42 -- X-Barracuda-BRTS-Status: 1 8, 42 -- X-Barracuda-Spam-Score: 0.00 8, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests= 8, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.28496 8, 42 -- Rule breakdown below 8, 42 -- pts rule name description 8, 42 -- ---- ---------------------- -------------------------------------------------- 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u36DIc2H006420 ==============================End of - Headers==============================
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Shadow casting using Denton Desk II sputter coater.
Message: We have Denton Desk II Sputter coater. It is old and basic model for sputter coating to prepare sample for SEM. I have one user, who is interested in study of extracellular matrix protein by shadow casting TEM. So, I wanted to know that what is the minimum thickness can be achieved with Denton Desk II sputter coater using what parameters. Here, we can have control on current and sputtering time only and we can get max vacuum of 100 mTorr.
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Your problem is most likely one of 2 things: 1) The wing surface is setose or highly setose and trapping air or water, thus preventing the resin from penetrating the wing cuticle. or 2) The lipids covering the external surface of the exoskeleton are also coating the wing surface and are not being removed during the alcohol dehydration steps.
For either case, I would first try adding a drop or two of non-ionic detergent (like Triton X-100 or Tween 80) to the fixative. This will act as a surfactant and help get the alcohol to the wing surface. It may also help solubilize the lipids coating the wing, helping with their removal. This may take some experimentation - a "drop or two" may not be enough. If the wings sink in the fixative, that is usually a good indication of "enough". But, you may need to use a more non-polar (organic) solvent to remove any lipid coating. With luck, a longer chain alcohol like tert-butyl will work.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
Message: We are trying to fix insect wings for TEM. It appears that something in the exoskeleton is making it harder wing to remain attached to the resin (of the block).
If you have any constructive suggestions, please send them to blayjorge-at-gmail.com
Gratefully,
Jorge
==============================Original Headers============================== 14, 35 -- From oshel1pe-at-cmich.edu Sat Apr 2 14:41:20 2016 14, 35 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 14, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u32JfKWO007593 14, 35 -- for {microscopy-at-microscopy.com} ; Sat, 2 Apr 2016 14:41:20 -0500 14, 35 -- Received: from cas.cmich.edu ([141.209.15.90]) 14, 35 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u32Jg7pM007911; 14, 35 -- Sat, 2 Apr 2016 15:42:07 -0400 14, 35 -- Received: from cmail106.central.cmich.local ([fe80::a8:3e31:c722:be0a]) by 14, 35 -- CAS3.central.cmich.local ([fe80::1e:4650:6064:41c5%10]) with mapi id 14, 35 -- 14.03.0248.002; Sat, 2 Apr 2016 15:42:09 -0400 14, 35 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 14, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 35 -- CC: "blayjorge-at-gmail.com" {blayjorge-at-gmail.com} 14, 35 -- Subject: RE: [Microscopy] viaWWW:Fixing Insects for TEM 14, 35 -- Thread-Topic: [Microscopy] viaWWW:Fixing Insects for TEM 14, 35 -- Thread-Index: AQHRjG4H8mGJ305xoE2ht+t6vzfv7p93E/b5 14, 35 -- Date: Sat, 2 Apr 2016 19:42:08 +0000 14, 35 -- Message-ID: {FB5ED57CF3415D4F8C5F93F1DE5468732FD00B2C-at-cmail106.central.cmich.local} 14, 35 -- References: {201604012326.u31NQS9D022599-at-ns.microscopy.com} 14, 35 -- In-Reply-To: {201604012326.u31NQS9D022599-at-ns.microscopy.com} 14, 35 -- Accept-Language: en-US 14, 35 -- Content-Language: en-US 14, 35 -- X-MS-Has-Attach: 14, 35 -- X-MS-TNEF-Correlator: 14, 35 -- x-originating-ip: [141.209.2.100] 14, 35 -- Content-Type: text/plain; charset="us-ascii" 14, 35 -- MIME-Version: 1.0 14, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 14, 35 -- X-Spam-Score: -4.70 () [Hold at 6.00] L_CTCMUM,L_EXCH_MF,RDNS_NONE,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 14, 35 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 14, 35 -- X-CanItPRO-Stream: default 14, 35 -- X-Canit-Stats-ID: 02QBvG7Xt - 24683a0f86c9 - 20160402 14, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 14, 35 -- Content-Transfer-Encoding: 8bit 14, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u32JfKWO007593 ==============================End of - Headers==============================
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Email: a.helvoort-at-ntnu.no Name: Ton van Helvoort
Organization: NTNU/TEM Gemini Centre
Title-Subject: [Filtered] Workshop microscopy data analysis with Python & HyperSpy
Message: The hands-on workshop, in connection with SCANDEM 2016, will address the basics of multi-dimensional data analysis using HyperSpy (http://hyperspy.org/). Most examples will be taken from the field of electron microscopy and will include EDX, EELS, image and diffraction analysis using conventional and machine learning methods. The same methodology should be directly applicable to other domains. No previous knowledge of the Python programming language is required.
Registration closes 1st May or when the places are filled. (Note: Early-bird registration and abstract submission deadline for SCANDEM: 8 April)
HyperSpy is a Python library developed by active microscopists allowing basic and advanced data analysis. The Python programming language is rapidly establishing itself as the lingua franca in most areas of scientific computing, including microscopy.
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Message: We are trying to fix insect wings for TEM. It appears that something in the exoskeleton is making it harder wing to remain attached to the resin (of the block).
If you have any constructive suggestions, please send them to blayjorge-at-gmail.com
Gratefully,
Jorge
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The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was purchased in 1984, it's price tag was ~ $1.5M. The instrument included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER, SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders, ......
After 20+ years, the instrument's "last light" was on Nov 19, 2008 and at decommissioning it was still operating at 300 kV. It's parts were redistributed and still populate several research instruments (some of which are not electron microscopes).
Nestor
-- ===========================================
Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Center for Nanoscale Materials NanoScience and Technology Division Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Center for Nanoscale Materials NanoScience and Technology Division Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
The box said ... "This program requires Win 95/98/NT or better..." So I bought a Mac !
===========================================
From: {John.Mardinly-at-asu.edu}
Yes, they were a lot cheaper when half the price of a microscope was not add-ons from Gatan. Of course, they did a lot less.
John Mardinly, Retired from ASU
On Mar 31, 2016, at 7:16 PM, bigelow-at-umich.edu wrote:
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In the early 1960s I purchased a JEOL JEM6A TEM that operated at 80 and 100 KV and was fully equipped (rotating-tilting stage, heating stage, tensile deformation stage, and even a Cine camera) for $35K. It had a guaranteed resolution of 20A, with a stated potential of reaching 10A under ideal external conditions. This microscope served the teaching and research needs of the graduate sstudents and faculty of our deartment for thirty years. This was one of the first TEMS to compete with the Siemans TEMs. Hitachi made a similar instrument that sold for a similar price. At about that time RCA sold a Model EML TEM that operated at 50KV, and was specifically designed for biological applications, at a much lower price.
==============================Original Headers============================== 1, 42 -- From bigelow-at-umich.edu Thu Mar 31 20:59:38 2016 1, 42 -- Received: from mail-io0-f174.google.com (mail-io0-f174.google.com [209.85.223.174]) 1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u311xcrs024870 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 20:59:38 -0500 1, 42 -- Received: by mail-io0-f174.google.com with SMTP id a129so127782352ioe.0 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 19:00:21 -0700 (PDT) 1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=umich.edu; s=google; 1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 1, 42 -- :content-transfer-encoding; 1, 42 -- bh=xDMen4it2GJ8Gg72E8ctRtQQrJuUICPQh8PMVw09Cs4=; 1, 42 -- b=BNvZ89dGEcglSaUHoAqeDAOuuZAGu0/K6TRjbql9kBMARqmOOLBm1udjwT8tWCwNe2 1, 42 -- 26a0KWMYB9sfm/etE2X/4ciV3/91YlDYwHt1IN3i3xHPWWTbVV3MQQYpdjefbTDJt4Sj 1, 42 -- IY6hMFC9a/aBY1SFH7sPsJSBPBWWMizHwz+48= 1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=1e100.net; s=20130820; 1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 1, 42 -- :subject:content-transfer-encoding; 1, 42 -- bh=xDMen4it2GJ8Gg72E8ctRtQQrJuUICPQh8PMVw09Cs4=; 1, 42 -- b=KAXAmJN9+veUBxRe5A2D5hpZQ7fJ2rftbXlNRt84gMcqv10jvHtL9/nUGc6tPG5r0Y 1, 42 -- p4hQ3igEzJzkA0/u5r7ZON7uS6jcs9ghq9dKOf8+lY6KIyfAYmE2PuLmndFoLgtu9NyR 1, 42 -- LOjiV/wklbOSYmzesrx1a1sWaXr64whAbfTHP1+iCLMV3k52acIglBB1p0XSRXXjYISg 1, 42 -- t8Sys4bg7lG/RbnMOstJAAlw9ZAFa1+pMzs+jeQEOAsrHwKMvvOwC7y7N0qss1qYTQbW 1, 42 -- Pmyc5cfO2PZNGpRuYR72djfS3FWOO/e6MbP4TVjm2tUPTobsYCEgm//31qwmjblOlRhC 1, 42 -- ZUCg== 1, 42 -- X-Gm-Message-State: AD7BkJK1ZhciThDezeVRExLef8B0ZmZL7xyGIoNlfRy1IjQygjNTC2BPS9MYc85p1TrC4Qob 1, 42 -- X-Received: by 10.107.136.140 with SMTP id s12mr4965943ioi.109.1459476021799; 1, 42 -- Thu, 31 Mar 2016 19:00:21 -0700 (PDT) 1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 1, 42 -- by smtp.gmail.com with ESMTPSA id 7sm11432942igt.6.2016.03.31.19.00.20 1, 42 -- for {microscopy-at-microscopy.com} 1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 1, 42 -- Thu, 31 Mar 2016 19:00:21 -0700 (PDT) 1, 42 -- Message-ID: {56FDD632.1020103-at-umich.edu} 1, 42 -- Date: Thu, 31 Mar 2016 22:00:18 -0400 1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 42 -- MIME-Version: 1.0 1, 42 -- To: microscopy-at-microscopy.com 1, 42 -- Subject: [Microscopy]Pricce of early EMs 1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 42 -- Content-Transfer-Encoding: 8bit 9, 40 -- From John.Mardinly-at-asu.edu Fri Apr 1 14:50:20 2016 9, 40 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) 9, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u31JoJgh024812 9, 40 -- for {Microscopy-at-Microscopy.com} ; Fri, 1 Apr 2016 14:50:19 -0500 9, 40 -- X-ASG-Debug-ID: 1459540263-0549c42950d74a0003-FOsErg 9, 40 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnetw3.asu.edu with ESMTP id h7V4cSSBjYgnoiQN (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 01 Apr 2016 12:51:04 -0700 (MST) 9, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 9, 40 -- X-Barracuda-Effective-Source-IP: exhubw01.asurite.ad.asu.edu[129.219.4.199] 9, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 9, 40 -- X-ASG-Whitelist: Client 9, 40 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 9, 40 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.03.0266.001; 9, 40 -- Fri, 1 Apr 2016 12:50:53 -0700 9, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 9, 40 -- To: "Wilbur C. Bigelow" {bigelow-at-umich.edu} , MSA {Microscopy-at-Microscopy.com} 9, 40 -- Subject: Re: [Microscopy] Pricce of early EMs 9, 40 -- Thread-Topic: [Microscopy] Pricce of early EMs 9, 40 -- X-ASG-Orig-Subj: Re: [Microscopy] Pricce of early EMs 9, 40 -- Thread-Index: AQHRi7yj3Uu9x4PPb0iMdYAsioBDpZ91/XcA 9, 40 -- Date: Fri, 1 Apr 2016 19:50:52 +0000 9, 40 -- Message-ID: {BEAFEBFC-B214-4208-B7F9-28F77A67A5A1-at-asu.edu} 9, 40 -- References: {201604010216.u312Gfch015283-at-ns.microscopy.com} 9, 40 -- In-Reply-To: {201604010216.u312Gfch015283-at-ns.microscopy.com} 9, 40 -- Accept-Language: en-US 9, 40 -- Content-Language: en-US 9, 40 -- X-MS-Has-Attach: 9, 40 -- X-MS-TNEF-Correlator: 9, 40 -- x-originating-ip: [129.219.103.4] 9, 40 -- Content-Type: text/plain; charset="utf-8" 9, 40 -- Content-ID: {F87F5FBA8CB4934FB189316F39862D70-at-exchange.asu.edu} 9, 40 -- MIME-Version: 1.0 9, 40 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] 9, 40 -- X-Barracuda-Start-Time: 1459540264 9, 40 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 9, 40 -- X-Barracuda-URL: https://149.169.2.73:443/cgi-mod/mark.cgi 9, 40 -- X-Barracuda-Scan-Msg-Size: 4565 9, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 9, 40 -- X-Barracuda-BRTS-Status: 1 9, 40 -- Content-Transfer-Encoding: 8bit 9, 40 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u31JoJgh024812 ==============================End of - Headers==============================
The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was purchased in 1984, it's price tag was ~ $1.5M. The instrument included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER, SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders, ......
After 20+ years, the instrument's "last light" was on Nov 19, 2008 and at decommissioning it was still operating at 300 kV. It's parts were redistributed and still populate several research instruments (some of which are not electron microscopes).
Nestor
-- ===========================================
Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Center for Nanoscale Materials NanoScience and Technology Division Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was purchased in 1984, it's price tag was ~ $1.5M. The instrument included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER, SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders, ......
After 20+ years, the instrument's "last light" was on Nov 19, 2008 and at decommissioning it was still operating at 300 kV. It's parts were redistributed and still populate several research instruments (some of which are not electron microscopes).
Nestor
-- ===========================================
Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Center for Nanoscale Materials NanoScience and Technology Division Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was purchased in 1984, it's price tag was ~ $1.5M. The instrument included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER, SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders, ......
After 20+ years, the instrument's "last light" was on Nov 19, 2008 and at decommissioning it was still operating at 300 kV. It's parts were redistributed and still populate several research instruments (some of which are not electron microscopes).
Nestor
-- ===========================================
Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Center for Nanoscale Materials NanoScience and Technology Division Bldg 212 / A-143 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
The JEM-6A was pretty old TEM. I used JEM-6C until 1985 for observation on biology samples. It used many vacuum tubes and big resistors and we have to put those things in separated room. On 1986, we bought JEM-1200EX with ASID10 (SEM/STEM attachment). I paid $450K.
Vincent Wang Schafer Vallecitos Laboratory
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In the early 1960s I purchased a JEOL JEM6A TEM that operated at 80 and 100 KV and was fully equipped (rotating-tilting stage, heating stage, tensile deformation stage, and even a Cine camera) for $35K. It had a guaranteed resolution of 20A, with a stated potential of reaching 10A under ideal external conditions. This microscope served the teaching and research needs of the graduate sstudents and faculty of our deartment for thirty years. This was one of the first TEMS to compete with the Siemans TEMs. Hitachi made a similar instrument that sold for a similar price. At about that time RCA sold a Model EML TEM that operated at 50KV, and was specifically designed for biological applications, at a much lower price.
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Email: prevedel-at-embl.de Name: Robert Prevedel
Organization: EMBL Heidelberg
Title-Subject: [Filtered] Application Engineer / Research Scientist Position in Optical Microscopy
Message: Job Description
The European Molecular Biology Laboratory (EMBL) is one of the highest ranked scientific research organisations in the world. The Headquarters Laboratory is located in Heidelberg (Germany), with additional sites in Grenoble (France), Hamburg (Germany), Hinxton (UK) and Monterotondo (Italy).
The Application Engineer / Research Scientist will join the Prevedel group. The goal of the lab is to develop next generation, innovative optical imaging techniques to push the frontier of deep tissue microscopy. The research is geared towards direct applications in biology and neuroscience. The major task of the Application Engineer / Research Scientist will be to support the group by contributing to its research projects and laboratory management. This involves: Design of mechanical, optical as well as electronic components of cutting-edge, novel light microscopes to collect three-dimensional datasets inside scattering tissue.
Construction and adaptation of custom microscopy setups for specific life science applications and imaging conditions. Programming and customizing computational data analysis packages including three-dimensional visualization of imaging data and analysis methods.
Improving image acquisition workflows (e.g. hardware automation, image post-processing and analysis) and ensuring a technology watch to keep the microscopes at a cutting edge level (at the hardware and software levels). Working in close collaboration with biologists and neuroscientists to ensure practicality of the microscopes (e.g. custom sample mounts, sample environment control)
The Application Engineer / Research Scientist will directly participate in the group s research projects and will be responsible for training and providing technical assistance for students and postdoctoral fellows in the group. In addition, the Application Engineer / Research Scientist will be in charge of the general lab organization that includes ordering of supplies and equipment as well as the maintenance of databases.
Qualifications and Experience
The ideal candidate should be an optical engineer or physicist (master degree or equivalent, a PhD would be an advantage) possessing advanced computer skills. The candidate should ideally have an expertise in advanced optics, imaging/spectroscopy or light microscopy, must be comfortable with complex technical equipment (including pulsed lasers) and preferably possess programming (Matlab, Python, LabView, or similar) and/or simulation skills (CAD, ZEMAX, or similar). A keen interest in biological applications of new optical technologies as well as experience in supervising students and laboratory organization would be an advantage.
The successful candidate should be able to work independently, be very well organized, reliable and enjoy working in an international team within a highly collaborative atmosphere. Excellent communication and presentation skills and fluency in English are required.
Application Instructions
Please apply online through www.embl.org/jobs
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EMBL is an inclusive, equal opportunity employer offering attractive conditions and benefits appropriate to an international research organisation.
Please note that appointments on fixed term contracts can be renewed, depending on circumstances at the time of the review.
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Nice answers... but nobody asked Ravi what is precisely is sample and this is an essential information for a sound answer!
First the optimum X-Ray line and beam energy will not be the same if the sample is - a bulk homogeneous material or - a thin (how thin) film on a bulk substrate or (micro/nano-) particles on a bulk substrate or even - - a thin foil like for TEM To be sure that you analyze the volume containing the elements you are interested in and only that volume you should run a Monte-Carlo simulation.
Second: The nature of the matrix may also influence the choice for X-ray line once you take the absorption consideration and possible overlap with adjacent lines from other elements.
When I started at Wool Research Organisation New Zealand in 2003 (now AgResearch), we had a gorgeous Philips EM300 which had been bought new in 1968. It kept working (with help in the form of some organ transplants from friends at The University of Otago) until 2006 when we decommissioned.
I have the brochure replete with photographs of an attractive young (female) scientist peering devotedly into the instrument and changing the wehnalt assembly etc. It was a top range 80-100 kV TEM of its day. The handwritten notes on the brochure margins indicate $41,000, presumably New Zealand Dollars (newly introduced the year before in a change from Pounds). When introduced a NZ$ was apparently valued at an NZ pound (which had been pegged to the British pound). Educated guess is therefore about GPB20,000 for the instrument back in the day.
Hopefully that stacks up.
Kind regards Duane
AgResearch Limited | Lincoln Research Centre | New Zealand Dr Duane P Harland, Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz
==============================Original Headers============================== 8, 35 -- From Duane.Harland-at-agresearch.co.nz Thu Mar 31 21:57:45 2016 8, 35 -- Received: from out1102.nz.smxemail.com (out1102.nz.smxemail.com [203.84.134.33]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u312vi1j024096 8, 35 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 21:57:44 -0500 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 8, 35 -- q=dns/txt; i=-at-nz.smxemail.com; t=1459479506; 8, 35 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 8, 35 -- bh=SQn/RaV1xq0DLJMO51/94tMgxI75rFE7tPuVn5zO+C4=; 8, 35 -- b=0H3jK7VdCT9IZURh+wHOyrshc4SB2VZLG4GsJ/yIVPuQiITYVVRIw670icAS/Ih1 8, 35 -- aZDztTnlCo/hrfbxlnWKXBrWArpY5RnQCkGh9+BLm0adRD8PcsUyifjGDq5FHtto 8, 35 -- s/yY2xnwwUCBRWnFlRaweqaizKcib+/rwB+yEOJDQ8c=; 8, 35 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 8, 35 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 8, 35 -- with cipher AES128-SHA (128/128 bits)) 8, 35 -- id 56FDE3D1-DBEB2FAC-at-mta1103.omr; 8, 35 -- Fri, 01 Apr 2016 02:58:26 +0000 8, 35 -- Received: from INVEXCPV01.agresearch.co.nz ([169.254.1.132]) by 8, 35 -- invexcpv03.agresearch.co.nz ([147.158.130.6]) with mapi id 14.03.0210.002; 8, 35 -- Fri, 1 Apr 2016 15:58:22 +1300 8, 35 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 8, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 35 -- Subject: RE: Pricce of early EMs 8, 35 -- Thread-Topic: Pricce of early EMs 8, 35 -- Thread-Index: AdGLwh3dLgkSOAjMRbS/7EgboSotjQ== 8, 35 -- Date: Fri, 1 Apr 2016 02:58:22 +0000 8, 35 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF1D560413B-at-invexcpv01.agresearch.co.nz} 8, 35 -- Accept-Language: en-GB, en-NZ, en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [147.158.192.122] 8, 35 -- Content-Type: text/plain; charset="iso-8859-1" 8, 35 -- MIME-Version: 1.0 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u312vi1j024096 ==============================End of - Headers==============================
In the early 1960s I purchased a JEOL JEM6A TEM that operated at 80 and 100 KV and was fully equipped (rotating-tilting stage, heating stage, tensile deformation stage, and even a Cine camera) for $35K. It had a guaranteed resolution of 20A, with a stated potential of reaching 10A under ideal external conditions. This microscope served the teaching and research needs of the graduate sstudents and faculty of our deartment for thirty years. This was one of the first TEMS to compete with the Siemans TEMs. Hitachi made a similar instrument that sold for a similar price. At about that time RCA sold a Model EML TEM that operated at 50KV, and was specifically designed for biological applications, at a much lower price.
==============================Original Headers============================== 1, 42 -- From bigelow-at-umich.edu Thu Mar 31 20:59:38 2016 1, 42 -- Received: from mail-io0-f174.google.com (mail-io0-f174.google.com [209.85.223.174]) 1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u311xcrs024870 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 20:59:38 -0500 1, 42 -- Received: by mail-io0-f174.google.com with SMTP id a129so127782352ioe.0 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 19:00:21 -0700 (PDT) 1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=umich.edu; s=google; 1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 1, 42 -- :content-transfer-encoding; 1, 42 -- bh=xDMen4it2GJ8Gg72E8ctRtQQrJuUICPQh8PMVw09Cs4=; 1, 42 -- b=BNvZ89dGEcglSaUHoAqeDAOuuZAGu0/K6TRjbql9kBMARqmOOLBm1udjwT8tWCwNe2 1, 42 -- 26a0KWMYB9sfm/etE2X/4ciV3/91YlDYwHt1IN3i3xHPWWTbVV3MQQYpdjefbTDJt4Sj 1, 42 -- IY6hMFC9a/aBY1SFH7sPsJSBPBWWMizHwz+48= 1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=1e100.net; s=20130820; 1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 1, 42 -- :subject:content-transfer-encoding; 1, 42 -- bh=xDMen4it2GJ8Gg72E8ctRtQQrJuUICPQh8PMVw09Cs4=; 1, 42 -- b=KAXAmJN9+veUBxRe5A2D5hpZQ7fJ2rftbXlNRt84gMcqv10jvHtL9/nUGc6tPG5r0Y 1, 42 -- p4hQ3igEzJzkA0/u5r7ZON7uS6jcs9ghq9dKOf8+lY6KIyfAYmE2PuLmndFoLgtu9NyR 1, 42 -- LOjiV/wklbOSYmzesrx1a1sWaXr64whAbfTHP1+iCLMV3k52acIglBB1p0XSRXXjYISg 1, 42 -- t8Sys4bg7lG/RbnMOstJAAlw9ZAFa1+pMzs+jeQEOAsrHwKMvvOwC7y7N0qss1qYTQbW 1, 42 -- Pmyc5cfO2PZNGpRuYR72djfS3FWOO/e6MbP4TVjm2tUPTobsYCEgm//31qwmjblOlRhC 1, 42 -- ZUCg== 1, 42 -- X-Gm-Message-State: AD7BkJK1ZhciThDezeVRExLef8B0ZmZL7xyGIoNlfRy1IjQygjNTC2BPS9MYc85p1TrC4Qob 1, 42 -- X-Received: by 10.107.136.140 with SMTP id s12mr4965943ioi.109.1459476021799; 1, 42 -- Thu, 31 Mar 2016 19:00:21 -0700 (PDT) 1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 1, 42 -- by smtp.gmail.com with ESMTPSA id 7sm11432942igt.6.2016.03.31.19.00.20 1, 42 -- for {microscopy-at-microscopy.com} 1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 1, 42 -- Thu, 31 Mar 2016 19:00:21 -0700 (PDT) 1, 42 -- Message-ID: {56FDD632.1020103-at-umich.edu} 1, 42 -- Date: Thu, 31 Mar 2016 22:00:18 -0400 1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 42 -- MIME-Version: 1.0 1, 42 -- To: microscopy-at-microscopy.com 1, 42 -- Subject: [Microscopy]Pricce of early EMs 1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 42 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Organization: Pacific Northwest National Laboratory
Title-Subject: [Filtered] Looking to purchase a JEOL 2010F gun/tank/microscope
Message: I am looking to find spare parts for a JEOL 200kV field emission TEM. In particular I am interested in the gun and the tank but I can also purchase the whole system if someone is interested in selling it. I would be willing to pay for all stages of the dismantling and shipping of the instrument to PNNL as well as a purchase price for the microscope. Any current condition for the microscope will be considered as we will be looking to rip it apart anyway.
Nigel
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia
Organization: University of Maryland, Baltimore
Title-Subject: [Filtered] UMB Current Electron Microscopy Techniques Workshop May 24 - 25, 2016
Message: Dear Colleagues,
We are pleased to announce that the University of Maryland Baltimore will be hosting the Third Annual Current EM Techniques Workshop on May 24 and 25, 2016. The purpose of this annual workshop is to introduce participants to new electron microscopy techniques and instruments via live demonstration and open discussion. The focus of this year s workshop is Correlative LM & EM. The workshop will include oral presentations and Tips-and-Tricks Technology forums on the first day and live instrument demonstration on the second day. Dr. Lucy Collison of Francis Crick Institute and Richard Schalek of Harvard University will be the keynote speakers. Demo instruments will feature ATUMtome of RMC-Boeckeler, ASP-1000-IGL of Microscopy Innovations, Pelco Biowave Pro Microwave System of TedPella, CryoEM sample preparation of Leica Microsystems, Polyo III, Lynx Tissue Processor of EMS, cryo-TEM, cryo-SEM. Live Serial Block Face sectioning will be viewed via weblink.
A dinner event will be co-sponsored by the Chesapeake Microscopy and Microanalysis society (CMMS) on May 24th providing opportunities for social and scientific exchange among workshop participants and CMMS members.
More information: http://www.dental.umaryland.edu/2016currentemtechniquesworkshop/
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XRF will be easier for detection since it won't have the same prominent background (bremstrallung) that electron beam excitation has. Therefore, peaks will be more detectable.
You should be using a lower voltage. The factor of 2.5 is a good rule of thumb. You should be able to see the Zn L line well enough with a thin window detector. I can hardly envision using a Be window detector anymore. I really like seeing the elements down to Be. I routinely depend on seeing the C and O lines. I spend most of my time at 10 kV anymore and sometimes drop it further.
However, I think you should be able to see peaks corresponding to a tenth percent or so by EDS. The limit will be how well the background is defined. I routinely collect at 15-20 kcps for a minute. I will count longer in critical situations where I need to improve the detection limit.
Hi Ravi, EDS is not the best to detect element in trace (if there is less than 1%). It's easier by fluorescence when the SEM has an Xray source or by WDS. Anyway I think you can do better to optimize the conditions because 30KV is not suitable (except to see the L alpha of Pb). An empiric rule says that the best accelerating voltage is 2.5 times the energy of the peak you expect to analyse. This is because the ionization efficiency of an atom by an electron is nearly maximum when the energy of the electron is between 2 or 3 times the energy of ionization. Applying this rule you may get more photons on : Pb M alpha near 5KV Pb L alpha near 30KV Cd L alpha near 9KV Zn K alpha near 24KV Zn L alpha near 3KV (but probably to low voltage to get a good count rate. So you can try 5KV) Once the accelerating voltage adjusted, increase the beam current to increase the count rate and acquire the spectrum during long time (may be 300 secondes).
Nicolas STEPHANT
Universite de Nantes Institut Jean Rouxel Service de microscopie electronique a balayage et microanalyse 2 rue de la Houssiniere BP 92208 44322 Nantes cedex 3
Tel : 02 40 37 64 26 Mel : nicolas.stephant-at-univ-nantes.fr Web : http://cnrs-imn.fr
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.
Message: I did EDS analysis by SEM and I was interested to find Pb,Cd and Zn elements but unfortunately my sample did not show peaks of any of these element. I used optimum parameters like 30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM Oxford EDS detector 10 mm2 window.
What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in concentration)? May I have a chart or any reference document where I can study the general detecting limit of EDS by TEM and SEM for all the elements.
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==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Mar 31 10:33:41 2016 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2VFXfTL024328 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 10:33:41 -0500 7, 26 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id u2VFYKMt011089 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 17:34:20 +0200 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id u2VFYJ0a025273 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 17:34:19 +0200 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- Subject: Re:Detecting limit by EDS elemental analysis. 7, 26 -- References: {201603302333.u2UNXHjj019506-at-ns.microscopy.com} 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Message-ID: {56FD4380.1020908-at-univ-nantes.fr} 7, 26 -- Date: Thu, 31 Mar 2016 17:34:24 +0200 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 7, 26 -- Thunderbird/38.5.1 7, 26 -- MIME-Version: 1.0 7, 26 -- In-Reply-To: {201603302333.u2UNXHjj019506-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 56FD437C.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 56FD437C.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
According to my old advisor, Will Bigelow, the JEOL 100CX TEM/STEM that I used for my thesis cost $300K back in 1977. Seemed like a lot of money back then.
John Mardinly, Retired from ASU
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Email: paulwebsterphd-at-gmail.com Name: Paul Webster
Organization: Oak Crest
Title-Subject: [Filtered] Cost of electron microscopes
Message: Hello listers,
I have a question posed by a colleague, which I think is better answered by the experts who read this. Here is the question:
"What do you know about the pricing of electron microscopes in the early days? NMR started to get really expensive in the late 1970's with the development of bigger and bigger superconducting magnets. I am guessing that EM reached the same price points earlier. Could you venture a guess about when it was that a commercial EM was first on the market for more than a million dollars?"
As a bonus question (from me), does anyone know when the first high-voltage microscopes came on to the market, and what their cost was?
I'm writing from the MSA submission form because I am unable to get through using the regular connection - I am subscribed so will see any messages posted to the list.
Thank you,
Paul Webster Oak Crest Institute of Science California
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format=flowed 19, 52 -- Content-Transfer-Encoding: 7bit 8, 40 -- From John.Mardinly-at-asu.edu Thu Mar 31 12:09:37 2016 8, 40 -- Received: from bcnetw3.asu.edu (bcnetw3.asu.edu [149.169.2.73]) 8, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u2VH9a29017560 8, 40 -- for {Microscopy-at-Microscopy.com} ; Thu, 31 Mar 2016 12:09:36 -0500 8, 40 -- X-ASG-Debug-ID: 1459444039-0549c414c86be360007-FOsErg 8, 40 -- Received: from exhubw02.asurite.ad.asu.edu (exhubw02.asurite.ad.asu.edu [129.219.4.200]) by bcnetw3.asu.edu with ESMTP id 7WEmQl3hrZwMpHmM (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Thu, 31 Mar 2016 10:07:34 -0700 (MST) 8, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 8, 40 -- X-Barracuda-Effective-Source-IP: exhubw02.asurite.ad.asu.edu[129.219.4.200] 8, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 8, 40 -- X-ASG-Whitelist: Client 8, 40 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 8, 40 -- exhubw02.asurite.ad.asu.edu ([129.219.4.200]) with mapi id 14.03.0266.001; 8, 40 -- Thu, 31 Mar 2016 10:07:14 -0700 8, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 8, 40 -- To: MSA {Microscopy-at-Microscopy.com} 8, 40 -- Subject: Re: [Microscopy] viaWWW: Historical Cost of electron microscopes 8, 40 -- Thread-Topic: [Microscopy] viaWWW: Historical Cost of electron microscopes 8, 40 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW: Historical Cost of electron microscopes 8, 40 -- Thread-Index: AQHRityxY3Ax9zVhckGDLr3yVK+qZp90PxkA 8, 40 -- Date: Thu, 31 Mar 2016 17:07:14 +0000 8, 40 -- Message-ID: {102E6509-1CC4-4ADA-9008-4B983AB5A1F7-at-asu.edu} 8, 40 -- References: {201603302333.u2UNXgcE020437-at-ns.microscopy.com} 8, 40 -- In-Reply-To: {201603302333.u2UNXgcE020437-at-ns.microscopy.com} 8, 40 -- Accept-Language: en-US 8, 40 -- Content-Language: en-US 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-originating-ip: [129.219.103.4] 8, 40 -- Content-Type: text/plain; charset="us-ascii" 8, 40 -- Content-ID: {5F67251D4F83D14EA93AC8B9B500727D-at-exchange.asu.edu} 8, 40 -- MIME-Version: 1.0 8, 40 -- X-Barracuda-Connect: exhubw02.asurite.ad.asu.edu[129.219.4.200] 8, 40 -- X-Barracuda-Start-Time: 1459444054 8, 40 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 8, 40 -- X-Barracuda-URL: https://149.169.2.73:443/cgi-mod/mark.cgi 8, 40 -- X-Barracuda-Scan-Msg-Size: 6767 8, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 8, 40 -- X-Barracuda-BRTS-Status: 1 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2VH9a29017560 ==============================End of - Headers==============================
Tommi; You can make a basket for metal by wrapping sone 600-700 micron tungsten wire around the end of a sheet metal screw, and just place the metal you want to evaporate in the basket. Use the same power supply used for carbon evaporation to heat the basket.
John Mardinly, Retired from ASU
On Mar 30, 2016, at 4:33 PM, microscopy.listserver-at-gmail.com wrote:
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Email: whiteto-at-missouri.edu Name: Tommi A. White
Organization: University of Missouri Electron Microscopy Core
Title-Subject: [Filtered] Thermal evaporation with Emitech Carbon Evaporator
Message: Dear Microscopy List-serve,
In the absence of funding, we would like to try to modify our turbo pumped Emitech equipment (from 1999) into a thermal evaporator allowing better adherence of metal coating for our electron beam lithography patterns.
We have both the sputtering system (K575X) and carbon evaporator (K950). In the carbon evaporator manual, it says we can use for thermal evaporation but there are little instructions on how to perform this conversion.
Does anyone have experience with converting this system for metal evaporation? Maybe a detailed description of how this conversion could take place? Would anyone be willing to guide us on what we need to do? We have an instrument repair shop and a machine shop on campus, so we can utilize their expertise if we know what we need.
Thanks in advance for any input. Tommi
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Hi Ravi, EDS is not the best to detect element in trace (if there is less than 1%). It's easier by fluorescence when the SEM has an Xray source or by WDS. Anyway I think you can do better to optimize the conditions because 30KV is not suitable (except to see the L alpha of Pb). An empiric rule says that the best accelerating voltage is 2.5 times the energy of the peak you expect to analyse. This is because the ionization efficiency of an atom by an electron is nearly maximum when the energy of the electron is between 2 or 3 times the energy of ionization. Applying this rule you may get more photons on : Pb M alpha near 5KV Pb L alpha near 30KV Cd L alpha near 9KV Zn K alpha near 24KV Zn L alpha near 3KV (but probably to low voltage to get a good count rate. So you can try 5KV) Once the accelerating voltage adjusted, increase the beam current to increase the count rate and acquire the spectrum during long time (may be 300 secondes).
Nicolas STEPHANT
Universite de Nantes Institut Jean Rouxel Service de microscopie electronique a balayage et microanalyse 2 rue de la Houssiniere BP 92208 44322 Nantes cedex 3
Tel : 02 40 37 64 26 Mel : nicolas.stephant-at-univ-nantes.fr Web : http://cnrs-imn.fr
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.
Message: I did EDS analysis by SEM and I was interested to find Pb,Cd and Zn elements but unfortunately my sample did not show peaks of any of these element. I used optimum parameters like 30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM Oxford EDS detector 10 mm2 window.
What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in concentration)? May I have a chart or any reference document where I can study the general detecting limit of EDS by TEM and SEM for all the elements.
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==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Mar 31 10:33:41 2016 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2VFXfTL024328 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 10:33:41 -0500 7, 26 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id u2VFYKMt011089 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 17:34:20 +0200 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id u2VFYJ0a025273 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT) 7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 17:34:19 +0200 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- Subject: Re:Detecting limit by EDS elemental analysis. 7, 26 -- References: {201603302333.u2UNXHjj019506-at-ns.microscopy.com} 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Message-ID: {56FD4380.1020908-at-univ-nantes.fr} 7, 26 -- Date: Thu, 31 Mar 2016 17:34:24 +0200 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 7, 26 -- Thunderbird/38.5.1 7, 26 -- MIME-Version: 1.0 7, 26 -- In-Reply-To: {201603302333.u2UNXHjj019506-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 56FD437C.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 56FD437C.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Besides the hardware upgrades over the decades, let's remember the software to run it all. Software is a big part of the package--who does not expect a good UI?
My 2 cents.
Roseann
Roseann Csencsits Group Leader-Microbeam Analyses Schafer Vallecitos Laboratories, Inc. 6705 Vallecitos Rd. Sunol, CA 94586 925-862-4345 roseann.csencsits-at-schafercorp.com
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, March 31, 2016 5:56 AM To: Csencsits, Roseann {roseann.csencsits-at-schafercorp.com}
EDS systems seem to me to be going up as well. I won't name companies, but I paid just over $65k in 2011 for the EDS system on my SEM, but was quoted $93k (after discount) for the EDS on my new S/TEM, and was recently quoted $147k+ for an EDS system on a FE-SEM. Some of this increase must be due to the change to SDD detectors from Si(Li) detectors, but not all of it.
Phil
On 03/30/2016 23:25 , joetherob-at-gmail.com wrote:
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Paul,
I have been in the commercial end of EM since the late 70's. I have worked for 4 different column companies and three EDS companies.
The price of a scanning electron microscope in the late seventies ranged from $15k for an ISI Mini SEM to $ 125,000 for a full featured SEM. TEMs were about $75,000 for a biological unit and $175,000 for a Philips 420 type System.
In the 80's the entry level SEMs were about $35 to 50,000. High end SEMs were still about $125,000. The onset of Technology changes (Field Emission SEMs and intermediate voltage TEMs) in about 1985 correlated migration of semiconductor companies to routine EM use. This saw the price of FE SEMs and IM TEMs to rise steeply. I was in sales for Philips in 1989 and a CM20 was about $400K while a CM30 was $500K. With the addition of STEM, EDS, and EELS, they could approach $900,000. On the SEM front, Hitachi took advantage of their early lead in field emission SEM by offering them at about $250,000.
Prices rose slowly through the 90s. I believe the first $ 1 million EMs were full 200 mm wafer inspection and CD SEMs. TEMs broke the barrier in the mid nineties with the introduction of Field Emission TEMs.
By the end of the nineties dualbeam systems were over $1,000,000. FE SEMs were up to $400K and TEMs started to hit the $1.5 million mark with the introduction of the Tecnai F30 and JEOL 2010F.
Now we see prices jump again in the early 2000s with the introduction of aberration corrected TEMs at 2 to $ 4 million. FE SEMs were up to $450K and a new generation of mini SEMs took hold at about $50,000. Prices have risen for newer technologies in the 2100s, while the cost of a full featured floor standing SEM has remained at about& 150,000.
Interestingly, the price of EDS has stayed at about the same price for 30 years: $45K.
Regards, Joe Robinson
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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EDS systems seem to me to be going up as well. I won't name companies, but I paid just over $65k in 2011 for the EDS system on my SEM, but was quoted $93k (after discount) for the EDS on my new S/TEM, and was recently quoted $147k+ for an EDS system on a FE-SEM. Some of this increase must be due to the change to SDD detectors from Si(Li) detectors, but not all of it.
Phil
On 03/30/2016 23:25 , joetherob-at-gmail.com wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Paul,
I have been in the commercial end of EM since the late 70's. I have worked for 4 different column companies and three EDS companies.
The price of a scanning electron microscope in the late seventies ranged from $15k for an ISI Mini SEM to $ 125,000 for a full featured SEM. TEMs were about $75,000 for a biological unit and $175,000 for a Philips 420 type System.
In the 80's the entry level SEMs were about $35 to 50,000. High end SEMs were still about $125,000. The onset of Technology changes (Field Emission SEMs and intermediate voltage TEMs) in about 1985 correlated migration of semiconductor companies to routine EM use. This saw the price of FE SEMs and IM TEMs to rise steeply. I was in sales for Philips in 1989 and a CM20 was about $400K while a CM30 was $500K. With the addition of STEM, EDS, and EELS, they could approach $900,000. On the SEM front, Hitachi took advantage of their early lead in field emission SEM by offering them at about $250,000.
Prices rose slowly through the 90s. I believe the first $ 1 million EMs were full 200 mm wafer inspection and CD SEMs. TEMs broke the barrier in the mid nineties with the introduction of Field Emission TEMs.
By the end of the nineties dualbeam systems were over $1,000,000. FE SEMs were up to $400K and TEMs started to hit the $1.5 million mark with the introduction of the Tecnai F30 and JEOL 2010F.
Now we see prices jump again in the early 2000s with the introduction of aberration corrected TEMs at 2 to $ 4 million. FE SEMs were up to $450K and a new generation of mini SEMs took hold at about $50,000. Prices have risen for newer technologies in the 2100s, while the cost of a full featured floor standing SEM has remained at about& 150,000.
Interestingly, the price of EDS has stayed at about the same price for 30 years: $45K.
Regards, Joe Robinson
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 32 -- From oshel1pe-at-cmich.edu Thu Mar 31 07:31:32 2016 4, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2VCVWZ3008571 4, 32 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 07:31:32 -0500 4, 32 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 4, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u2VCWB03024379 4, 32 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2016 08:32:11 -0400 4, 32 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 4, 32 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 4, 32 -- (TLS) id 14.3.248.2; Thu, 31 Mar 2016 08:32:12 -0400 4, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 4, 32 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 31 Mar 4, 32 -- 2016 08:32:11 -0400 4, 32 -- Message-ID: {56FD18CB.8050907-at-cmich.edu} 4, 32 -- Date: Thu, 31 Mar 2016 08:32:11 -0400 4, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 4, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 32 -- MIME-Version: 1.0 4, 32 -- To: micro {microscopy-at-microscopy.com} 4, 32 -- Subject: Re: [Microscopy] Historical prices of EMs 4, 32 -- References: {201603310325.u2V3P60e023490-at-ns.microscopy.com} 4, 32 -- In-Reply-To: {201603310325.u2V3P60e023490-at-ns.microscopy.com} 4, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- X-Originating-IP: [141.209.2.100] 4, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 32 -- X-Spam-Score: 0.80 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,US_DOLLARS_3,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 4, 32 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 4, 32 -- X-CanItPRO-Stream: default 4, 32 -- X-Canit-Stats-ID: 02QAAwbFO - bc9115018727 - 20160331 4, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I have been in the commercial end of EM since the late 70's. I have worked for 4 different column companies and three EDS companies.
The price of a scanning electron microscope in the late seventies ranged from $15k for an ISI Mini SEM to $ 125,000 for a full featured SEM. TEMs were about $75,000 for a biological unit and $175,000 for a Philips 420 type System.
In the 80's the entry level SEMs were about $35 to 50,000. High end SEMs were still about $125,000. The onset of Technology changes (Field Emission SEMs and intermediate voltage TEMs) in about 1985 correlated migration of semiconductor companies to routine EM use. This saw the price of FE SEMs and IM TEMs to rise steeply. I was in sales for Philips in 1989 and a CM20 was about $400K while a CM30 was $500K. With the addition of STEM, EDS, and EELS, they could approach $900,000. On the SEM front, Hitachi took advantage of their early lead in field emission SEM by offering them at about $250,000.
Prices rose slowly through the 90s. I believe the first $ 1 million EMs were full 200 mm wafer inspection and CD SEMs. TEMs broke the barrier in the mid nineties with the introduction of Field Emission TEMs.
By the end of the nineties dualbeam systems were over $1,000,000. FE SEMs were up to $400K and TEMs started to hit the $1.5 million mark with the introduction of the Tecnai F30 and JEOL 2010F.
Now we see prices jump again in the early 2000s with the introduction of aberration corrected TEMs at 2 to $ 4 million. FE SEMs were up to $450K and a new generation of mini SEMs took hold at about $50,000. Prices have risen for newer technologies in the 2100s, while the cost of a full featured floor standing SEM has remained at about & 150,000.
Interestingly, the price of EDS has stayed at about the same price for 30 years: $45K.
Regards, Joe Robinson
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Perhaps the easiest way to look at detection limits is to use one of the Monte Carlo spectrum simulation programs. There are a number of programs available but you might want to try...
Nicholas Ritchie (NIST) DTSA-II - http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/
One caution about this software. They use the same models and databases as the standardless quantification routines, so the same uncertainties apply to the simulation as to standardless quant. That said, they can be useful to get an idea of detection limits and experimental feasibility.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
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X-from: ravi.thakkar369-at-gmail.com
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.
Message: I did EDS analysis by SEM and I was interested to find Pb,Cd and Zn elements but unfortunately my sample did not show peaks of any of these element. I used optimum parameters like 30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM Oxford EDS detector 10 mm2 window.
What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in concentration)? May I have a chart or any reference document where I can study the general detecting limit of EDS by TEM and SEM for all the elements.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.
Message: I did EDS analysis by SEM and I was interested to find Pb,Cd and Zn elements but unfortunately my sample did not show peaks of any of these element. I used optimum parameters like 30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM Oxford EDS detector 10 mm2 window.
What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in concentration)? May I have a chart or any reference document where I can study the general detecting limit of EDS by TEM and SEM for all the elements.
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Email: paulwebsterphd-at-gmail.com Name: Paul Webster
Organization: Oak Crest
Title-Subject: [Filtered] Cost of electron microscopes
Message: Hello listers,
I have a question posed by a colleague, which I think is better answered by the experts who read this. Here is the question:
"What do you know about the pricing of electron microscopes in the early days? NMR started to get really expensive in the late 1970's with the development of bigger and bigger superconducting magnets. I am guessing that EM reached the same price points earlier. Could you venture a guess about when it was that a commercial EM was first on the market for more than a million dollars?"
As a bonus question (from me), does anyone know when the first high-voltage microscopes came on to the market, and what their cost was?
I'm writing from the MSA submission form because I am unable to get through using the regular connection - I am subscribed so will see any messages posted to the list.
Thank you,
Paul Webster Oak Crest Institute of Science California
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Email: whiteto-at-missouri.edu Name: Tommi A. White
Organization: University of Missouri Electron Microscopy Core
Title-Subject: [Filtered] Thermal evaporation with Emitech Carbon Evaporator
Message: Dear Microscopy List-serve,
In the absence of funding, we would like to try to modify our turbo pumped Emitech equipment (from 1999) into a thermal evaporator allowing better adherence of metal coating for our electron beam lithography patterns.
We have both the sputtering system (K575X) and carbon evaporator (K950). In the carbon evaporator manual, it says we can use for thermal evaporation but there are little instructions on how to perform this conversion.
Does anyone have experience with converting this system for metal evaporation? Maybe a detailed description of how this conversion could take place? Would anyone be willing to guide us on what we need to do? We have an instrument repair shop and a machine shop on campus, so we can utilize their expertise if we know what we need.
Thanks in advance for any input. Tommi
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The EPMA 2016 Topical Conference held at the University of Wisconsin, Madison May 16 - 19 can provide up to $750 in reimbursement for travel, lodging, and registration through a NSF grant. The NSF funds available for ECS financial support can only be used for awardees in the USA. ECS includes undergraduates, graduates, post docs, and professionals within 2 years of their terminal degree. No abstract is required to receive the funds. However, a letter of support from an advisor/employer/supervisor is needed and an example is provided below. Awards will be granted on a weekly basis until the funds have been fully committed.
Conference content preview: - EPMA, SEM, WDS, EDS techniques - Tutorial on EPMA and SEM quantitative analysis - Topics with invited and contributed presentations.
Confirmed invited speakers: * C. MacRae (Australia - CL and low energy line) * K. Goemann (Tasmania - shared background by WDS) * P. Statham (England - state-of-the-art quantitative analysis by EDS) * X. Llovet (Spain - Monte Carlo simulation, low voltage analysis) * J. Donovan (OR - Overview of analytical techniques) * A. Moy (France - Virtual standards) * E. Vicenzi (MD - Standards and reference materials) * M.J. Jercinovic (MA - trace element analysis by EPMA) ...and more! - Discussion and group meeting - Problems identified by the microanalysis community vs. solutions provided by the vendor community - Presentations and product information from the microanalysis vendor community - Inexpensive housing options - Group meals
Example email which can be used to propose an ECS candidate to EPMA 2016
Send to: Paul.carpenter.epma-at-gmail.com hlowers-at-usgs.gov
X-from: m.aindow-at-uconn.edu
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Email: m.aindow-at-uconn.edu Name: Mark Aindow
Organization: University of Connecticut
Title-Subject: [Filtered] Postdoctoral Position in Electron Microscopy
Message: The Institute of Materials Science at UConn is an interdisciplinary center with the threefold mission of fostering education, research and outreach in all areas of the materials sciences. The Institute is serving as the temporary home for the UConn-FEI Center for Advanced Microscopy and Materials Analysis (CAMMA), which includes facilities for scanning and transmission electron microscopy and focused ion beam instruments (see: http://www.ims.uconn.edu/uconn-fei-center-of-excellence-in-microscopy-a-tech-park-strategic-partnership/). CAMMA will move into its permanent home in mid-2017 upon completion of the new Innovation Partnership Building in the UConn Technology Park.
There is an opening in the Institute for an electron microscopy specialist. The appointee will be involved in a range of academic and industrial projects, and will assist in the operation of the Laboratory including: offering short courses, performing routine maintenance, and training/assisting users of the instruments. Candidates must hold a PhD in Materials Science or a related discipline and must have extensive hands-on experience in SEM, FIB and TEM. Experience with electron backscatter diffraction, aberration-corrected S/TEM, serial-section FIB tomography, and maintenance of instrumentation would also be beneficial. This is a fixed-term appointment (one-year University Postdoctoral Fellow in the first instance) and is available from May 1st. Screening of the applications will begin immediately and will continue until the post is filled. Applications from under-represented groups, including minorities, women and persons with disabilities are encouraged.
Interested candidates should send a curriculum vitae and the names of at least three referees with postal addresses, telephone numbers and Email addresses to: Prof. Mark Aindow, Institute of Materials Science, University of Connecticut, 97 North Eagleville Road, Storrs, CT 06269-3136 USA. Email: m.aindow-at-uconn.edu
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The deadline for submitting communications for the Congress Ultrapath XVIII in Lisbon has been extended one last time to April 23. Do not lose this opportunity to join your colleagues in an updated overview of Ultrastructural Pathology.
Your work is very important for the Society and and your contribution to the Congress will help all of us to keep improving and spread the word about the continued importance of Ultrastructural Pathology.
With kind regards,
From the Organizing Committee, A.P. Alves de Matos Chair of UltrapathXVIII
http://congress.ultrapathXVIII.org
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] high-pressure purifier for carbon dioxide/CPD unit
Message: Our critical-point dryer currently uses a Union Carbide model SG-6140 high-pressure purifier for the carbon dioxide. Our stock of the replacement filters is nearly depleted and UC/Dow apparently doesn t produce these parts any longer.
Does anyone know of an available cross-reference filter that will fit this UC purifier? Or can anyone recommend an alternative high-pressure purifier that has readily available parts?
thank you-
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I am the Publications Officer for the Australian Microscopy and Microanalysis Society and am asking for a little help. I am trying to complete the collection of the Society's publications and am one volume short of a complete collection. The volume I am missing is Book 2 of the Proceedings of the 5th Australian Conference on Electron Microscopy (ACEM) held in Adelaide in 1978.
If you have this volume I am more than willing to pay for postage, scan the volume and send it back to you or if you would prefer to scan it and email it to me that would be wonderful.
The collection at present is a combination of physical and electronic volumes but I am in the process of producing electronic versions of all the publications.
Thank you very much for your assistance with this matter.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
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Email: jarnikm-at-mail.nih.gov Name: Michael
Organization: NIH
Title-Subject: [Filtered] anti-GFP antibody for immunocytochemistry
Message: Hi Listers,
I would like to hear tips for a good anti-GFP antibody that would work for immunocytochemistry (Tokuyasu). I have been using a poyclonal Invitrogene, but although specific, the sensitivity is rather low. Please feel free to contact me off-list, if you prefer so.
Thanks, Michael
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Did he explain what his goal was in doing this? That would certainly help to know. Indeed, I do not go far in supporting unusual requests unless and until the user is willing to explain themselves. I have had too many occasions where what they wanted to do was actually quite available with another technique that they did not know about.
The Quanta affords a movie option that can be programmed at whatever capture rate they want. Stage X&Y is one of the options for the databar so it could be used to track moves.
I suppose there are some screen capture utilities that could render a movie of the whole screen showing the changes to other parameters. I hope it has a good level of compression. Most of the screen would not be changing from frame to frame and should not take up much of the data stream.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, March 21, 2016 6:46 PM To: Straszheim, Warren E [BIOTC]
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Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] Data logger for FEI Quanta 450
Message: I have a user who wants to document everything he does while using our FEI Quanta 450 FEGSEM. A call to FEI Tech Support said they can log error messages, but they can't log other actions such as moving sample etc.
My question is, does anyone have an app, or know a company that might sell such an app, that will enable my user to document all his actions on the scope?
Thanks in advance
Steve Barlow SDSU EM Facility
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What you are asking for is called "FIB Assist" and was/is made by Fibics in Ottawa, but I doubt it would be commercially available for Quanta.
For documenting FIB process development work or circuit edits I use stand-alone video recorder, configured to capture FIB screen at about three frames per second. Separate PC with Epiphan PCIe is my favorite solution, but you can get USB-pluggable version from the same guys or elsewhere:
Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
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Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] Data logger for FEI Quanta 450
Message: I have a user who wants to document everything he does while using our FEI Quanta 450 FEGSEM. A call to FEI Tech Support said they can log error messages, but they can't log other actions such as moving sample etc.
My question is, does anyone have an app, or know a company that might sell such an app, that will enable my user to document all his actions on the scope?
Thanks in advance
Steve Barlow SDSU EM Facility
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If your alcohol concentrations are correct - as you use them, then I would suspect that the jump from 6.95% to 100% is hydrating the 100% far more than would occur if you used:
My routine - assuming that the number of steps is fixed - dehydrates the tissues far better than the one you're are using, again, if your numbers are correct. Water is almost always the culprit in mechanical processors.
I have NO experience with vacuum processing for light microscopy.
I have stored tissues in 100% EtOH for months in -20C freezer and for years at -80C with no perceptible increase in hardening. 200 Proof EtOH (sorry, ethanol!) becomes 95% EtOH rapidly when dispensed from gallon containers. I used to pour my once-used first and second 100% EtOHs into the first and second 95% containers for the next run. Fresh 100% for every run.
If your Xylene shows any white mist, water is doing it, and miscibility with paraffin will be degraded.
Hope this helps,
Cheers,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work) Fc.monson-at-gmail.com 610-331-8322 (Mobile-Text)
-----Original Message----- X-from: Charles Riley via Histonet [mailto:histonet-at-lists.utsouthwestern.edu] Sent: Tuesday, March 22, 2016 11:27 AM To: histonet-at-lists.utsouthwestern.edu
X-from: sbarlow-at-mail.sdsu.edu
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Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] Data logger for FEI Quanta 450
Message: I have a user who wants to document everything he does while using our FEI Quanta 450 FEGSEM. A call to FEI Tech Support said they can log error messages, but they can't log other actions such as moving sample etc.
My question is, does anyone have an app, or know a company that might sell such an app, that will enable my user to document all his actions on the scope?
Thanks in advance
Steve Barlow SDSU EM Facility
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Did anyone work with proteasome samples in TEM? Currently I have a user that brought these samples in, and we prepared and stained with uranyl acetate. The contrast isn't that great. Before I go trying other options around, I decided to check if any of you have a better experience with the sample and a different chemical for staining.
Thanks in advance for all help.
Regards,
Marcela.
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I keep the alcohols dry with dialysis tubing filled with sieve, and separate tubes of cupric sulfate as an indicator. It will turn blue when water is present. Roughly annually I have to recharge the sieve in the oven at 275C overnight and recharge the CS by heating in a crucible. I usually use a plumbers torch with spread flame- or whenever I run the self-cleaning on the oven I pop last years in. When it turns white it is ready to go. Been using the same material 15 years now.
Scott Whittaker Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
-----Original Message----- X-from: peter.heimann-at-uni-bielefeld.de [mailto:peter.heimann-at-uni-bielefeld.de] Sent: Friday, March 18, 2016 8:11 AM To: Whittaker, Scott {WHITTAKS-at-si.edu}
Dear colleagues,
for drying ("removal of bound water") of 3A-molecular sieve the "web" recommends at least 300* Celsius ( 572* Fahrenheit resp.).
Would 250* C (482* F) for several hours be sufficient ?
has anybody tried to dry molecular sieve in high vacuum? in microwave oven ?
and has anybody a suggestion for a simple test / indicator if there are traces of water in ethanol or acetone ?
I would be interested in any technical information/system documentation for the EDAX Apollo X SDD detector and the EDAX TEAM software. The detector has a "SIGNAL" output which I suppose outputs the pulses.
How is this signal acquired and processed on the PC ? Is there some kind of EDAX signal acquisition card ?
Thank you, Stefan
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"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------
Peter,
I've been using 200 - 250 deg C for "several hours" to dry molecular sieve for years. Works a treat. I do make sure to have some molecular sieve that has an indicator dye mixed in with the regular. It will revert to the "blue = dry" state in the oven, but it does wear out over time and needs to be refreshed.
No handy test for traces of water, sorry. But, I do keep molecular sieve in my absolute ethanol. Either lose and handle with great care to not stir up dust, or in dialysis tubing.
Phil
Dear colleagues,
for drying ("removal of bound water") of 3A-molecular sieve the "web" recommends at least 300* Celsius ( 572* Fahrenheit resp.).
Would 250* C (482* F) for several hours be sufficient ?
has anybody tried to dry molecular sieve in high vacuum? in microwave oven ?
and has anybody a suggestion for a simple test / indicator if there are traces of water in ethanol or acetone ?
thanks for a short informal reply,
Yours,
Peter
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 32 -- From oshel1pe-at-cmich.edu Fri Mar 18 07:39:02 2016 6, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2ICd226028895 6, 32 -- for {microscopy-at-microscopy.com} ; Fri, 18 Mar 2016 07:39:02 -0500 6, 32 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged)) 6, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u2ICd5Ld017120; 6, 32 -- Fri, 18 Mar 2016 08:39:07 -0400 6, 32 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 6, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: oshel1pe-at-cmich.edu To: colijn.1-at-osu.edu Sent: 3/18/2016 8:41:30 AM
Peter,
I've been using 200 - 250 deg C for "several hours" to dry molecular sieve for years. Works a treat. I do make sure to have some molecular sieve that has an indicator dye mixed in with the regular. It will revert to the "blue = dry" state in the oven, but it does wear out over time and needs to be refreshed.
No handy test for traces of water, sorry. But, I do keep molecular sieve in my absolute ethanol. Either lose and handle with great care to not stir up dust, or in dialysis tubing.
Phil
Dear colleagues,
for drying ("removal of bound water") of 3A-molecular sieve the "web" recommends at least 300* Celsius ( 572* Fahrenheit resp.).
Would 250* C (482* F) for several hours be sufficient ?
has anybody tried to dry molecular sieve in high vacuum? in microwave oven ?
and has anybody a suggestion for a simple test / indicator if there are traces of water in ethanol or acetone ?
thanks for a short informal reply,
Yours,
Peter
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 32 -- From oshel1pe-at-cmich.edu Fri Mar 18 07:39:02 2016 6, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2ICd226028895 6, 32 -- for {microscopy-at-microscopy.com} ; Fri, 18 Mar 2016 07:39:02 -0500 6, 32 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged)) 6, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u2ICd5Ld017120; 6, 32 -- Fri, 18 Mar 2016 08:39:07 -0400 6, 32 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 6, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with
I've been using 200 - 250 deg C for "several hours" to dry molecular sieve for years. Works a treat. I do make sure to have some molecular sieve that has an indicator dye mixed in with the regular. It will revert to the "blue = dry" state in the oven, but it does wear out over time and needs to be refreshed.
No handy test for traces of water, sorry. But, I do keep molecular sieve in my absolute ethanol. Either lose and handle with great care to not stir up dust, or in dialysis tubing.
Phil
Dear colleagues,
for drying ("removal of bound water") of 3A-molecular sieve the "web" recommends at least 300* Celsius ( 572* Fahrenheit resp.).
Would 250* C (482* F) for several hours be sufficient ?
has anybody tried to dry molecular sieve in high vacuum? in microwave oven ?
and has anybody a suggestion for a simple test / indicator if there are traces of water in ethanol or acetone ?
thanks for a short informal reply,
Yours,
Peter
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 32 -- From oshel1pe-at-cmich.edu Fri Mar 18 07:39:02 2016 6, 32 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2ICd226028895 6, 32 -- for {microscopy-at-microscopy.com} ; Fri, 18 Mar 2016 07:39:02 -0500 6, 32 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged)) 6, 32 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u2ICd5Ld017120; 6, 32 -- Fri, 18 Mar 2016 08:39:07 -0400 6, 32 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 6, 32 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 6, 32 -- (TLS) id 14.3.248.2; Fri, 18 Mar 2016 08:39:07 -0400 6, 32 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 6, 32 -- (141.209.15.141) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 18 Mar 6, 32 -- 2016 08:39:07 -0400 6, 32 -- Message-ID: {56EBF6E5.9090605-at-cmich.edu} 6, 32 -- Date: Fri, 18 Mar 2016 08:39:01 -0400 6, 32 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 32 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 32 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 32 -- MIME-Version: 1.0 6, 32 -- To: {peter.heimann-at-uni-bielefeld.de} , micro {microscopy-at-microscopy.com} 6, 32 -- Subject: Re: [Microscopy] drying of 3A molecular sieve ( Peter Heimann ) 6, 32 -- References: {201603181230.u2ICUjEI024218-at-ns.microscopy.com} 6, 32 -- In-Reply-To: {201603181230.u2ICUjEI024218-at-ns.microscopy.com} 6, 32 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 32 -- Content-Transfer-Encoding: 8bit 6, 32 -- X-Originating-IP: [141.209.2.100] 6, 32 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 32 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 6, 32 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 6, 32 -- X-CanItPRO-Stream: default 6, 32 -- X-Canit-Stats-ID: 02QvoD7mi - cfc128dd3953 - 20160318 6, 32 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: kwylly-at-ppg.com Name: Kelly Wylly
Organization: PPG
Title-Subject: [Filtered] Overlaying EDS Spectra
Message: What are the general rules that should be followed when overlaying EDS Spectra? Can you overlay spectra taken at two different sampling sizes as long as the counts are similar?
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: CHANL UNC
Title-Subject: [Filtered] Re live image to external monitor
Message: Thanks everyone (especially John M) who chimed in with ideas on how to get the SEM image out and to broadcast to a remote imaging class. We started out getting the image to the external monitor using VCN software. Then we used Lync to open remote sessions with local schools. Recently we are using Hangout to get around older operating system issues. Works adequately but there is still a good deal of lag time in image refresh. Still the students seem to be enjoying the sessions. Wallace
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Title-Subject: [Filtered] Northern California Society for Microscopy Spring Meeting April 21
Message: The Northern California Society of Microscopy invites you to its spring meeting hosted by the San Joaquin Delta College Microscopy Program in Stockton, CA.
3 - 3:30 pm Checkin at the Delta College Microscopy Building
3:30 - 5:30 pm Posters Mini Platform Presentations Vendor Displays
6 - 6:50 pm Buffet Dinner - showcasing the SJDC Culinary Arts Program
7 - 8 pm MSA Featured speaker: C. Barry Carter
"The Future of TEM and why we must Remember the Past"
RSVP: info-at-ncsmicroscopy.org by April 11. Sorry but late responders cannot be seated for dinner.
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Email: Desertrat99-at-verizon.net Name: Eric
Organization: UCLA
Title-Subject: [Filtered] Clinical EM Labs case loads?
Message: Hi to the group,
I work in a clinical EM Lab doing mostly renal biopsies. I was curious to know what other labs do for a workload in term of cases per year? How many are scoped by the Pathologist and how many are scoped by the tech?
In our currently there are just me and another tech. Our workload is approximately 1075 cases. We scope the cases for the pathologists.
Now they want to add 800 cases to our workload to process, cut and scope.
Thanks for your help.
"You only get one sunrise and one sunset a day, and you only get so many days on the planet. A good photographer does the math and doesn t waste either." Galen Rowell
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We are advertising a 2-year postdoc position in mapping electromagnetic fields using electron microscopy (experiment, theory or both) at School of Physics and Astronomy, Monash University, Australia. Further details below.
Please bring this position to the attention of anyone who might be interested.
Many thanks, Scott Findlay
___________________
Position overview: The Postdoctoral Research Fellow will work on high resolution transmission electron microscopy imaging of electromagnetic fields in materials on a project funded by the Australian Research Council ("Nanoscale field mapping in functional materials").
Duration: Two-year fixed-term appointment
Remuneration package: $68,186 - $92,541 pa Level A / $97,411 - $115,678 pa Level B (includes 9.50% employer superannuation)
Closing date: Friday 29th April, 2016
Position Descriptions and application details at: http://www.jobs-monash.jxt.net.au/academic-jobs/postdoctoral-research-fellow-in-electron-microscopy/600759
-- *SCOTT FINDLAY* QEII Fellow
*School of Physics and Astronomy* Monash University G08, New Horizons Centre, Clayton Campus 20 Research Way Clayton VIC 3800 Australia
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop at UGA
Message: Biological TEM Workshop July 6-8, 2016
This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment.
When: Wednesday, July 6 through Friday, July 8, 2016, 8am-5pm each day
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. gem.uga.edu
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Title-Subject: [Filtered] Help to measure Area, phase fraction and size
Message: I have a SEM image of four phases nickel, carbon,pores & YSZ. I want to determine microstructural properties of each phase
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Email: jmcnulty-at-luc.edu Name: John McNulty
Organization: Loyola University Chicago
Title-Subject: [Filtered] Blocks of deep-sea fishes
Message: I am retiring and am faced with throwing out epon-embedded tissues from a variety of deep sea fish species. If anyone would like them, let me know before April 1. John
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The only thing I can think of is this one: glue solubilized in solution, coated the stubs. Insufficient washing (or forgotten because of super bowl this evening?) so enough glue remained on the stubs and insulated them.
As for the economics of cleaning: Hard to believing that throwing 100 stubs in acetone and wating for the shaker to do its job is worth more than 2 $. Just don't throw each stub at a time in 100 solutions on 100 shakers and clean them sequentially :-)
Regards Stephane
-------------------------------------------- On Thu, 3/10/16, baskin-at-bio.umass.edu {baskin-at-bio.umass.edu} wrote:
Colleagues,
A year or two ago, we managed to make a set of insulating stubs by cleaning them. Our cleaning procedure was much as described in the last few days (razor blade scrabe, followed by solvent shaking, followed by grinding). I cannot really fathom how the stubs lost conductivity (and batches we cleaned previously were fine) but lose it they did. Set us back months troubleshooting that one. Perhaps a cautionary tale in addition to the economic argument brought up below?
As ever,
Tobias Biology Department, UMass Amherst, MA, 01003, USA
On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote:
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This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.
Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.
One also has to factor in the environmental cost of chemicals and disposal. Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.
We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)
So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.
Mike Toalson VP Sales & Marketing Ted Pella, Inc. PO Box 492477 * Redding, CA 96049 Tel: 530-243-2200 x205 * Cell: 530-356-5921 mike_toalson-at-tedpella.com * www.tedpella.com
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 7:15 AM To: Mike Toalson
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
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Email: skmeno1-at-nps.edu Name: SARATH MENON
Organization: NAVAL POSTGRADUATE SCHOOL
Title-Subject: [Filtered] Research Assistant Professor at Naval Postgraduate School, Monterey, CA
Message:
Research Assistant Professor, AD-1701-03 Department of Mechanical and Aerospace Engineering Naval Postgraduate School, Monterey, CA
The Mechanical and Aerospace Engineering Department of the Naval Postgraduate School is inviting applications from electron Microscopy Specialists to fulfill a Research Faculty position to support the research and development of a variety of materials including metals, ceramics, carbon-based materials and composites. The position will be focused on the operation of several advanced characterization techniques, including fully equipped Scanning Electron Microscope and Transmission Electron Microscope. Responsibilities of the position include the maintenance and upkeep of these complex analytical instruments. The applicant should possess operational expertise with SEM, FIB, TEM lamellae preparation, TEM operation including STEM methods, high resolution TEM and analytical techniques such as EDS, EBSD, EELS and EFTEM. The candidate for the position must be able to perform sample analysis with minimal direction and/or oversight from technical principal investigators. The candidate must also be able to collect, assimilate, and interpret data for technical principal investigators and write technical reports to document results. The position description includes also the coordination of all services required to keep instruments operational, the procurement of all consumables for the microscopy lab and to carry out all the routine maintenance tasks. A PhD in Materials Science or a closely related field is required. A few years of hands-on experience with current state-of-the-art instrumentation and techniques in the area of electron microscopy (SEM, TEM, STEM, EFTEM and FIB) is mandatory. The ability to teach a course related to Advanced Materials Characterization techniques will be highly valued. In addition, the candidate is expected to train Masters level students to use instruments and help them with data analysis. The ability to obtain a US security clearance is mandatory.
Applications should be received by 1 April 2015 and include a curriculum vitae, writing sample, summary of available teaching evaluations, and three letters of recommendation. Please address the applications to:
Garth V. Hobson Professor and Chair Department of Mechanical & Aerospace Engineering 700 Dyer Road, Bldg 245 Watkins Hall, WA-338 Naval Postgraduate School Monterey, CA 93943 TEL: 831-656-2586/831-656-2888 www.nps.edu/mae gvhobson-at-nps.edu
Salary is commensurate with qualifications and experience. Relocation package, including recruitment/relocation incentive may be authorized. The position will remain open until filled. The Naval Postgraduate School is an equal opportunity employer. For additional information about NPS, please refer to the website at http://www.nps.edu.
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave
Organization: DTU Nanotech
Title-Subject: [Filtered] PhD scholarship in Electron Microscopic Quantification of Nanoparticle Aerosols and Condensation behavior
Message: This project will develop electron microscopy and optical microscopy imaging-based and spectroscopic methods to establish new ways for aerosol characterization to provide information in high resolution. Aerosol samples and their changes with time and environmental conditions will also be observed during in-situ condensation experiments. Such methods will lead to a better understanding of aerosol sources, their composition and influence of environmental conditions, health effects and influence on climate.
The position is for three years at the Technical University of Denmark
info on: http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=3facbea3-4ecc-43c0-8414-490d9c092b3c
deadline 17th march 2016
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Email: fei.long-at-queensu.ca Name: Fei Long
Title-Subject: [Filtered] TEM- Gatan Enfinium EELS spectrum energy shift
Message: Hello all,
We are encountering an problem with our Gatan Enfinium spectrometer recently. The Autopeels panel can only capture EELS zero loss peak in the DM software. When we want to shift to higher energy levels, it simply shift the ZLP to the that energy. I d like to ask if anyone have an idea how I can solve this problem? Thanks,
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Email: g.h.ten.brink-at-rug.nl Name: Gert ten Brink
Organization: RUG/ZIAM
Title-Subject: [Filtered] SEM: cleaning used specimen mounts
Message: Dear Jaci, I do not even bother to clean them. They are consumables. Mind you, if you don't clean the old ones properly you make your SEM/vacuum dirty. If you buy them via the internet for a 100 pieces they cost 23.- euro's
The time spent cleaning them cost more.
My 2 cents, Gert
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Working essentially as the only full time person in an SEM lab, long ago I came to the conclusion that unless the cleaning was very quick and easy, it was more practical to simply recycle the small stubs. I could not justify much time to clean them. I do think the larger items are a different story, however. My metallurgical mount holders, vices, multi-stub holders, etc., are pricey and well worth the effort to keep them looking good, both for myself and as well as my clients. For these items, what I can't remove by hand, I ultrasonically clean first with an organic solvent. Once that is done and the parts are dry, I then ultrasonically clean them in a solution of warm water with Alconox powder. I've had surprisingly good success with this. It removes sputtered materials and other residues nicely. The holders come out looking great again with no signs of etching or attack on the aluminum. I've never tried any of the other Alconox products, however.
I have no stake in Alconox.
Chris Holp Sr. Nuclear Specialist BETA Labs Mayfield Village OH 44143 440-604-9704 holpc-at-firstenergycorp.com
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==============================Original Headers============================== 10, 61 -- From holpc-at-firstenergycorp.com Fri Mar 11 07:37:38 2016 10, 61 -- Received: from gw19.firstenergycorp.com (gw19.firstenergycorp.com [205.132.74.180]) 10, 61 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2BDbcet014479 10, 61 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2016 07:37:38 -0600 10, 61 -- Received: from WEXCH04P.fenetwork.com (unknown [205.132.74.4]) by gw19.firstenergycorp.com with smtp 10, 61 -- (TLS: TLSv1/SSLv3,256bits,ECDHE-RSA-AES256-SHA) 10, 61 -- id 1f6e_46f4_78d1e355_284e_465a_a17d_a833ae2070de; 10, 61 -- Fri, 11 Mar 2016 08:37:22 -0500 10, 61 -- Received: from WEXCH02P.fenetwork.com (172.32.99.148) by 10, 61 -- WEXCH04P.fenetwork.com (172.32.99.149) with Microsoft SMTP Server (TLS) id 10, 61 -- 15.0.847.32; Fri, 11 Mar 2016 08:36:06 -0500 10, 61 -- Received: from wxchgeg04p.fenetwork.com (148.108.32.19) by 10, 61 -- WEXCH02P.fenetwork.com (172.32.99.148) with Microsoft SMTP Server (TLS) id 10, 61 -- 15.0.847.32 via Frontend Transport; Fri, 11 Mar 2016 08:36:07 -0500 10, 61 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (207.46.163.141) 10, 61 -- by smtp.firstenergycorp.com (148.108.32.19) with Microsoft SMTP Server (TLS) 10, 61 -- id 15.0.847.32; Fri, 11 Mar 2016 08:36:06 -0500 10, 61 -- Received: from BN3PR0501MB1460.namprd05.prod.outlook.com (10.160.117.16) by 10, 61 -- BN3PR0501MB1460.namprd05.prod.outlook.com (10.160.117.16) with Microsoft SMTP 10, 61 -- Server (TLS) id 15.1.434.16; Fri, 11 Mar 2016 13:36:05 +0000 10, 61 -- Received: from BN3PR0501MB1460.namprd05.prod.outlook.com ([10.160.117.16]) by 10, 61 -- BN3PR0501MB1460.namprd05.prod.outlook.com ([10.160.117.16]) with mapi id 10, 61 -- 15.01.0434.016; Fri, 11 Mar 2016 13:36:05 +0000 10, 61 -- From: "Holp, Christopher R." {holpc-at-firstenergycorp.com} 10, 61 -- To: "Microscopy Listserver (Microscopy-at-Microscopy.com)" 10, 61 -- {Microscopy-at-microscopy.com} 10, 61 -- Subject: SEM: cleaning used specimen mounts 10, 61 -- Thread-Topic: SEM: cleaning used specimen mounts 10, 61 -- Thread-Index: AdF7mQ0zfTPM4sD1SHmxUnpXYH0cCQ== 10, 61 -- Date: Fri, 11 Mar 2016 13:36:05 +0000 10, 61 -- Message-ID: {BN3PR0501MB1460ED7D4ECA5AD08337A574BBB50-at-BN3PR0501MB1460.namprd05.prod.outlook.com} 10, 61 -- Accept-Language: en-US 10, 61 -- Content-Language: en-US 10, 61 -- X-MS-Has-Attach: 10, 61 -- X-MS-TNEF-Correlator: 10, 61 -- authentication-results: Microscopy.com; dkim=none (message not signed) 10, 61 -- header.d=none;Microscopy.com; dmarc=none action=none 10, 61 -- header.from=firstenergycorp.com; 10, 61 -- x-originating-ip: [148.108.208.35] 10, 61 -- x-ms-office365-filtering-correlation-id: d188861c-3464-41a6-53ff-08d349b21878 10, 61 -- x-microsoft-exchange-diagnostics: 1;BN3PR0501MB1460;5:70sG5iobBeRPhLll9pLVXXH0NoceVCvJ20uM0tkTfEhNHQAi5nRX9UzkpxSzXrysQ1Rze1hvaMmprZzzpDld6uSPk3T+5syXb7esEchBd+rpF49qeM5brZgceOTTnaRKeRiwPbF3ERzyt1Cc1RwoZA==;24:zKKcRJFXZ1TOYJ83xNekGWfNyosk2Kb+S37psNSPYv6UGqjwPZpcOI/lf8+J7zk4Gsu2WpwCdgXl+S3L0cBeP1Yi3wkVTSwDyEysXtKcaXc= 10, 61 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BN3PR0501MB1460; 10, 61 -- x-microsoft-antispam-prvs: {BN3PR0501MB1460DBBC6F906D5468D6D148BBB50-at-BN3PR0501MB1460.namprd05.prod.outlook.com} 10, 61 -- x-exchange-antispam-report-test: UriScan:; 10, 61 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(5005006)(8121501046)(3002001)(10201501046);SRVR:BN3PR0501MB1460;BCL:0;PCL:0;RULEID:;SRVR:BN3PR0501MB1460; 10, 61 -- x-forefront-prvs: 087894CD3C 10, 61 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(77096005)(122556002)(11100500001)(5003600100002)(33656002)(10400500002)(2900100001)(19580405001)(5002640100001)(81166005)(3660700001)(2906002)(450100001)(5008740100001)(229853001)(6116002)(1220700001)(102836003)(5004730100002)(99286002)(1096002)(586003)(3280700002)(92566002)(189998001)(3846002)(107886002)(110136002)(74316001)(76576001)(19580395003)(54356999)(50986999)(87936001)(86362001)(66066001);DIR:OUT;SFP:1101;SCL:1;SRVR:BN3PR0501MB1460;H:BN3PR0501MB1460.namprd05.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 10, 61 -- spamdiagnosticoutput: 1:23 10, 61 -- spamdiagnosticmetadata: NSPM 10, 61 -- Content-Type: text/plain; charset="us-ascii" 10, 61 -- MIME-Version: 1.0 10, 61 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 11 Mar 2016 13:36:05.1823 10, 61 -- (UTC) 10, 61 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 10, 61 -- X-MS-Exchange-CrossTenant-id: cf52e7e1-a48f-421c-898a-023b05eb044c 10, 61 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN3PR0501MB1460 10, 61 -- X-OriginatorOrg: firstenergycorp.com 10, 61 -- X-NAIMIME-Disclaimer: 1 10, 61 -- X-NAIMIME-Modified: 1 10, 61 -- Content-Transfer-Encoding: 8bit 10, 61 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2BDbcet014479 ==============================End of - Headers==============================
Working essentially as a one person SEM lab, long ago I came to the conclusion that unless the cleaning was very quick and easy, it was more practical to simply recycle the small stubs. I could not justify much time to clean them. I do think the larger items are a different consideration, however. My metallurgical mount holders, vices, multi-stub holders, etc., are pricey and well worth an effort to keep them looking good, both for myself and clients. For these, I remove paints and tape residues ultrasonically with an organic solvent. Once that is done, I've had decent success by ultrasonically cleaning them in a warm water solution with Alconox powder. This removes sputtered materials and other residuals nicely. The holders come out looking great again with no signs of etching or attack on the aluminum. I've never tried any of the other Alconox products for this, however.
(I have no stake in Alconox.)
Chris Holp Sr. Nuclear Specialist BETA Labs Mayfield Village OH 44143 440-604-9704, holpc-at-firstenergycorp.com
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Just a thought, but for a multiuser facility, it might not make economic sense for a microscopist to clean them, but it might be a good facility-level fix to put all the used stubs in a box and make it available to users with an SOP. That way, anybody needing and lacking one can grab one from the box and clean it themselves. It takes care of the labour aspect at least, and is essentially immediate recycling.
-Jake Jokisaari
On 03/10/2016 03:14 PM, oshel1pe-at-cmich.edu wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
We routinely clean off old stubs. Partly because they're easily cleaned and reused, partly because we have an nice source of free undergrad labor. Waiting for your next dehydration step? Clean stubs!
The chemical costs aren't great, and the cost-recovery of recycling the aluminum isn't great either, so cleaning seems best. Plus, there's no shipping and handling costs for cleaned stubs vs. new ones.
But so far we haven't managed to make insulating stubs ... you sure you didn't offend Mho, the Spirit of Conductivity, Tobias?
Phil
Colleagues, A year or two ago, we managed to make a set of insulating stubs by cleaning them. Our cleaning procedure was much as described in the last few days (razor blade scrabe, followed by solvent shaking, followed by grinding). I cannot really fathom how the stubs lost conductivity (and batches we cleaned previously were fine) but lose it they did. Set us back months troubleshooting that one. Perhaps a cautionary tale in addition to the economic argument brought up below?
As ever, Tobias Biology Department, UMass Amherst, MA, 01003, USA
On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.
Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.
One also has to factor in the environmental cost of chemicals and disposal. Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.
We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)
So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.
Mike Toalson VP Sales& Marketing Ted Pella, Inc. PO Box 492477 Redding, CA 96049 Tel: 530-243-2200 x205 Cell: 530-356-5921 mike_toalson-at-tedpella.com www.tedpella.com
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 7:15 AM To: Mike Toalson
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 3, 23 -- From baskin-at-bio.umass.edu Thu Mar 10 13:55:23 2016 3, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AJtNJ0002168 3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:55:23 -0600 3, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 3, 23 -- (authenticated bits=0) 3, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u2AJt7SL025437 3, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 3, 23 -- Thu, 10 Mar 2016 14:55:07 -0500 3, 23 -- Subject: Re: [Microscopy] RE: SEM: cleaning used specimen mounts 3, 23 -- To: mike_toalson-at-tedpella.com, microscnet {microscopy-at-microscopy.com} 3, 23 -- References: {201603101914.u2AJE2hj013333-at-ns.microscopy.com} 3, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 3, 23 -- Message-ID: {56E1D11B.1040804-at-bio.umass.edu} 3, 23 -- Date: Thu, 10 Mar 2016 14:55:07 -0500 3, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 3, 23 -- Gecko/20100101 Thunderbird/38.6.0 3, 23 -- MIME-Version: 1.0 3, 23 -- In-Reply-To: {201603101914.u2AJE2hj013333-at-ns.microscopy.com} 3, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Thu, 10 Mar 2016 14:55:07 -0500 (EST) 3, 23 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
We routinely clean off old stubs. Partly because they're easily cleaned and reused, partly because we have an nice source of free undergrad labor. Waiting for your next dehydration step? Clean stubs!
The chemical costs aren't great, and the cost-recovery of recycling the aluminum isn't great either, so cleaning seems best. Plus, there's no shipping and handling costs for cleaned stubs vs. new ones.
But so far we haven't managed to make insulating stubs ... you sure you didn't offend Mho, the Spirit of Conductivity, Tobias?
Phil
Colleagues, A year or two ago, we managed to make a set of insulating stubs by cleaning them. Our cleaning procedure was much as described in the last few days (razor blade scrabe, followed by solvent shaking, followed by grinding). I cannot really fathom how the stubs lost conductivity (and batches we cleaned previously were fine) but lose it they did. Set us back months troubleshooting that one. Perhaps a cautionary tale in addition to the economic argument brought up below?
As ever, Tobias Biology Department, UMass Amherst, MA, 01003, USA
On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.
Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.
One also has to factor in the environmental cost of chemicals and disposal. Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.
We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)
So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.
Mike Toalson VP Sales& Marketing Ted Pella, Inc. PO Box 492477 Redding, CA 96049 Tel: 530-243-2200 x205 Cell: 530-356-5921 mike_toalson-at-tedpella.com www.tedpella.com
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 7:15 AM To: Mike Toalson
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 3, 23 -- From baskin-at-bio.umass.edu Thu Mar 10 13:55:23 2016 3, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AJtNJ0002168 3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:55:23 -0600 3, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 3, 23 -- (authenticated bits=0) 3, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u2AJt7SL025437 3, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 3, 23 -- Thu, 10 Mar 2016 14:55:07 -0500 3, 23 -- Subject: Re: [Microscopy] RE: SEM: cleaning used specimen mounts 3, 23 -- To: mike_toalson-at-tedpella.com, microscnet {microscopy-at-microscopy.com} 3, 23 -- References: {201603101914.u2AJE2hj013333-at-ns.microscopy.com} 3, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 3, 23 -- Message-ID: {56E1D11B.1040804-at-bio.umass.edu} 3, 23 -- Date: Thu, 10 Mar 2016 14:55:07 -0500 3, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 3, 23 -- Gecko/20100101 Thunderbird/38.6.0 3, 23 -- MIME-Version: 1.0 3, 23 -- In-Reply-To: {201603101914.u2AJE2hj013333-at-ns.microscopy.com} 3, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Thu, 10 Mar 2016 14:55:07 -0500 (EST) 3, 23 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
Long ago, I came to the same conclusion for the stubs. Unless the cleaning was very quick and easy, I recycled the small stubs. I could not justify much time to clean them. I do think the larger items are a different consideration, however. My metallurgical mount holders, vices, multi-stub holders, etc. are well worth it. For these, I remove paints and tape residues ultrasonically with an organic solvent. Once that is done, I've had decent success by ultrasonically cleaning them in a warm water solution with Alconox powder. This removes sputtered materials well enough and seems to be inhibited enough to not attack the aluminum. I've never tried any of the other Alconox products for this, however.
(I have no stake in Alconox.)
Chris Holp Sr. Nuclear Specialist BETA Labs Mayfield Village OH 44143 440-604-9704, holpc-at-firstenergycorp.com
-----Original Message----- X-from: mike_toalson-at-tedpella.com [mailto:mike_toalson-at-tedpella.com] Sent: Thursday, March 10, 2016 2:34 PM To: Holp, Christopher R. {holpc-at-firstenergycorp.com}
This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.
Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.
One also has to factor in the environmental cost of chemicals and disposal. Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.
We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)
So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.
Mike Toalson VP Sales & Marketing Ted Pella, Inc. PO Box 492477 . Redding, CA 96049 Tel: 530-243-2200 x205 . Cell: 530-356-5921 mike_toalson-at-tedpella.com . www.tedpella.com
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 7:15 AM To: Mike Toalson
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59)
Colleagues, A year or two ago, we managed to make a set of insulating stubs by cleaning them. Our cleaning procedure was much as described in the last few days (razor blade scrabe, followed by solvent shaking, followed by grinding). I cannot really fathom how the stubs lost conductivity (and batches we cleaned previously were fine) but lose it they did. Set us back months troubleshooting that one. Perhaps a cautionary tale in addition to the economic argument brought up below?
As ever, Tobias Biology Department, UMass Amherst, MA, 01003, USA
On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.
Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.
One also has to factor in the environmental cost of chemicals and disposal. Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.
We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)
So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.
Mike Toalson VP Sales & Marketing Ted Pella, Inc. PO Box 492477 Redding, CA 96049 Tel: 530-243-2200 x205 Cell: 530-356-5921 mike_toalson-at-tedpella.com www.tedpella.com
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 7:15 AM To: Mike Toalson
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 3, 23 -- From baskin-at-bio.umass.edu Thu Mar 10 13:55:23 2016 3, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AJtNJ0002168 3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:55:23 -0600 3, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 3, 23 -- (authenticated bits=0) 3, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u2AJt7SL025437 3, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 3, 23 -- Thu, 10 Mar 2016 14:55:07 -0500 3, 23 -- Subject: Re: [Microscopy] RE: SEM: cleaning used specimen mounts 3, 23 -- To: mike_toalson-at-tedpella.com, microscnet {microscopy-at-microscopy.com} 3, 23 -- References: {201603101914.u2AJE2hj013333-at-ns.microscopy.com} 3, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 3, 23 -- Message-ID: {56E1D11B.1040804-at-bio.umass.edu} 3, 23 -- Date: Thu, 10 Mar 2016 14:55:07 -0500 3, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 3, 23 -- Gecko/20100101 Thunderbird/38.6.0 3, 23 -- MIME-Version: 1.0 3, 23 -- In-Reply-To: {201603101914.u2AJE2hj013333-at-ns.microscopy.com} 3, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Thu, 10 Mar 2016 14:55:07 -0500 (EST) 3, 23 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.
Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.
One also has to factor in the environmental cost of chemicals and disposal. Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.
We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)
So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.
Mike Toalson VP Sales & Marketing Ted Pella, Inc. PO Box 492477 Redding, CA 96049 Tel: 530-243-2200 x205 Cell: 530-356-5921 mike_toalson-at-tedpella.com www.tedpella.com
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 7:15 AM To: Mike Toalson
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59)
Hello, I just had the same problem today at a Jeol 5900LV at Munich. For the communication problem: I suppose your SEM also connects to the PC via SCSI cable? If yes, check at the Adaptec SCSI card at Bootup (CTRL+A) if the SCSI ID is the same like at the back of the SEM console. Adaptec card should list connect like "JeolSEM ID1" during bootup. If you get an error during launch of the SEM control program, check for the SCSI card to be bound to the appropriate "JEOL PCSEM SCSI Processor Device" driver to be loaded. Otherwise you won`t get a connect to the SEM. Driver should have come with your installation software by Jeol. Don`t forget to first switch the SEM on and then checking for the SCSI problems. ;-)
Best, Stefan
Am 10.03.2016 um 19:15 schrieb yqin-at-buffalo.edu: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Listers,
Recently our JEOL SEM PC motherboard failed, the PC is almost 20 years old, running Windows 2000. However, the PC still has communication problems with SEM after replacing the Mother Board. we are not sure whether the PC has other problems due to the failure of the Mother Board.
We were wondering whether anybody has a similar situation, and how you solve this problem? Can we just replace a new PC, and install the current SEM software to the new PC?
If anybody can help, please contact me. Thank you!
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Recently our JEOL SEM PC motherboard failed, the PC is almost 20 years old, running Windows 2000. However, the PC still has communication problems with SEM after replacing the Mother Board. we are not sure whether the PC has other problems due to the failure of the Mother Board.
We were wondering whether anybody has a similar situation, and how you solve this problem? Can we just replace a new PC, and install the current SEM software to the new PC?
If anybody can help, please contact me. Thank you! -- Dr. Yueling Qin Senior Research Support Specialist UB2020 Integrated Nanostructured Systems Initiative, University at Buffalo, SUNY, Buffalo, NY 14260, Phone (716) 645-8698
==============================Original Headers============================== 4, 32 -- From yqin-at-buffalo.edu Thu Mar 10 12:06:47 2016 4, 32 -- Received: from mtareserve1.acsu.buffalo.edu (mtareserve15.acsu.buffalo.edu [128.205.6.12]) 4, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AI6lBD001604 4, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 12:06:47 -0600 4, 32 -- Received: from localmailg.acsu.buffalo.edu (localmailg.acsu.buffalo.edu [128.205.4.25]) 4, 32 -- by mtareserve1.acsu.buffalo.edu (Postfix) with ESMTP id BA059E44D03 4, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:06:30 -0500 (EST) 4, 32 -- Received: from localmailg.acsu.buffalo.edu (localhost [127.0.0.1]) 4, 32 -- by localhost (Postfix) with SMTP id AD6ADE09B0 4, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:06:30 -0500 (EST) 4, 32 -- Received: from localmailg.acsu.buffalo.edu (localhost [127.0.0.1]) 4, 32 -- by localmailg.acsu.buffalo.edu (Postfix) with ESMTP id 8E381E09AF 4, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:06:30 -0500 (EST) 4, 32 -- Received: from smtp.buffalo.edu (smtp4.acsu.buffalo.edu [128.205.5.229]) 4, 32 -- by localmailg.acsu.buffalo.edu (Prefixe) with ESMTP id 8C586E09AE 4, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:06:30 -0500 (EST) 4, 32 -- Received: from ubmail.buffalo.edu (mail-lb-snat.acsu.buffalo.edu [128.205.6.28]) 4, 32 -- by smtp.buffalo.edu (Postfix) with ESMTP id 7D25CE2FEDF 4, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:06:30 -0500 (EST) 4, 32 -- MIME-Version: 1.0 4, 32 -- Content-Type: text/plain; charset=US-ASCII; 4, 32 -- format=flowed 4, 32 -- Content-Transfer-Encoding: 7bit 4, 32 -- Date: Thu, 10 Mar 2016 13:06:30 -0500 4, 32 -- From: Yueling Qin {yqin-at-buffalo.edu} 4, 32 -- To: Microscopy-at-microscopy.com 4, 32 -- Subject: JEOL SEM PC failed 4, 32 -- Organization: UB 4, 32 -- Message-ID: {9bbf4ebd2198900124839cc27a7a639c-at-buffalo.edu} 4, 32 -- X-Sender: yqin-at-buffalo.edu 4, 32 -- User-Agent: Roundcube Webmail/1.1.2 4, 32 -- X-PM-EL-Spam-Prob: : 8% ==============================End of - Headers==============================
For cleaning large quantities of mounts, shaker is very useful to minimize, if not eliminate the scrubbing.
Either "real" lab shaker if you have one, or pneumatic type used for paint mixing. Put used mounts into container (steel, SST, Al, PTFE, etc...), fill with acetone, put in the shaker, and leave running in fume hood either overnight or even over the weekend. Soaking for a day prior to putting in shaker may work better. Or run during the day and switch off overnight for safety. Can change acetone and shake little more if lots of carbon tape was used. Rinse with alcohol, dry, and re-use with virtually no scrubbing.
Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 3/10/2016 9:52 AM, LettJ-at-ent.wustl.edu wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u2AEd2OL014951 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 21fvmye7km-1 8, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 8, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 8, 35 -- Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 35 -- Subject: SEM: cleaning used specimen mounts 8, 35 -- Thread-Topic: SEM: cleaning used specimen mounts 8, 35 -- Thread-Index: AdF62+76Cjrsn7dqRqSD4UcP70OFVw== 8, 35 -- Date: Thu, 10 Mar 2016 14:48:49 +0000 8, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F0D084-at-pcfmbx01.wusm-pcf.wustl.edu} 8, 35 -- Accept-Language: en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [10.39.135.61] 8, 35 -- Content-Type: text/plain; charset="us-ascii" 8, 35 -- MIME-Version: 1.0 8, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.38,0.0.0000 8, 35 -- definitions=2016-03-10_08:2016-03-10,2016-03-10,1970-01-01 signatures=0 8, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 8, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 8, 35 -- engine=7.0.1-1601100000 definitions=main-1603100240 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2AEn6pA015697 ==============================End of - Headers==============================
Most of the adhesives and paints are soluble in acetone or methanol, including tape gums, carbon paint, silver paint, and cyanoacrylate glues. Isopropanol and ethanol can also work, but require more scrubbing, as it just softens the tape adhesive. I have had success in cleaning batches of SEM stubs by soaking them in acetone, wiping any tape off, then sonicating them in acetone or alcohol. Any harder residues can then be removed with a quick brush over fine sandpaper.
It is a bit time consuming, but it has worked pretty well for me.
Best,
Jake Jokisaari PhD Graduate University of Michigan
On 03/10/2016 09:11 AM, LettJ-at-ent.wustl.edu wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u2AEd2OL014951 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 21fvmye7km-1 8, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 8, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 8, 35 -- Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 35 -- Subject: SEM: cleaning used specimen mounts 8, 35 -- Thread-Topic: SEM: cleaning used specimen mounts 8, 35 -- Thread-Index: AdF62+76Cjrsn7dqRqSD4UcP70OFVw== 8, 35 -- Date: Thu, 10 Mar 2016 14:48:49 +0000 8, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F0D084-at-pcfmbx01.wusm-pcf.wustl.edu} 8, 35 -- Accept-Language: en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [10.39.135.61] 8, 35 -- Content-Type: text/plain; charset="us-ascii" 8, 35 -- MIME-Version: 1.0 8, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.38,0.0.0000 8, 35 -- definitions=2016-03-10_08:2016-03-10,2016-03-10,1970-01-01 signatures=0 8, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 8, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 8, 35 -- engine=7.0.1-1601100000 definitions=main-1603100240 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2AEn6pA015697 ==============================End of - Headers==============================
Hello Everyone, I scalp the stub with a single edge razor blade to remove as much as possible of the tape and adhesive and then polish the remaining adhesive with on a paper towel with a couple drops of CHCl3. I just place the paper on a table top, drop chloroform on it and holding the stub by the back end and polish the adhesive off. Change spots and replace solvent as needed.
Doing this as soon as possible after removing from the SEM is best. The adhesive and tape seems to harden in air after beam exposure.
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 10:09 AM To: Frank Karl
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u2AEd2OL014951 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 21fvmye7km-1 8, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 8, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 8, 35 -- Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 35 -- Subject: SEM: cleaning used specimen mounts 8, 35 -- Thread-Topic: SEM: cleaning used specimen mounts 8, 35 -- Thread-Index: AdF62+76Cjrsn7dqRqSD4UcP70OFVw== 8, 35 -- Date: Thu, 10 Mar 2016 14:48:49 +0000 8, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F0D084-at-pcfmbx01.wusm-pcf.wustl.edu} 8, 35 -- Accept-Language: en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [10.39.135.61] 8, 35 -- Content-Type: text/plain; charset="us-ascii" 8, 35 -- MIME-Version: 1.0 8, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.38,0.0.0000 8, 35 -- definitions=2016-03-10_08:2016-03-10,2016-03-10,1970-01-01 signatures=0 8, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 8, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 8, 35 -- engine=7.0.1-1601100000 definitions=main-1603100240 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2AEn6pA015697 ==============================End of - Headers==============================
Depending on what the mounting medium was, you may be able to remove it with a solvent, although it will probably still require some elbow grease. Sonicating the mounts may help speed up the process. Conductive tape is removable if you take it off after the SEM examination is complete, but the longer it stays on the mount, the more difficult it becomes to remove.
If you have access to metallography prep equipment, the easiest thing to do is just grind the top of the mount down on an abrasive disc, exposing fresh aluminum. Then, depending on the surface you want, you can leave it with a rough finish, or use finer grits to get a more polished surface.
Hope that helps some!
Jeff Jeffrey A. Hall | Microscopist SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA 1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Thursday, March 10, 2016 10:09 AM To: Jeff Hall {jhall-at-2spi.com}
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u2AEd2OL014951 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 21fvmye7km-1 8, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 8, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 8, 35 -- Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 35 -- Subject: SEM: cleaning used specimen mounts 8, 35 -- Thread-Topic: SEM: cleaning used specimen mounts 8, 35 -- Thread-Index: AdF62+76Cjrsn7dqRqSD4UcP70OFVw== 8, 35 -- Date: Thu, 10 Mar 2016 14:48:49 +0000 8, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F0D084-at-pcfmbx01.wusm-pcf.wustl.edu} 8, 35 -- Accept-Language: en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [10.39.135.61] 8, 35 -- Content-Type: text/plain; charset="us-ascii" 8, 35 -- MIME-Version: 1.0 8, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.38,0.0.0000 8, 35 -- definitions=2016-03-10_08:2016-03-10,2016-03-10,1970-01-01 signatures=0 8, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 8, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 8, 35 -- engine=7.0.1-1601100000 definitions=main-1603100240 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2AEn6pA015697 ==============================End of - Headers==============================
Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016 8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AEn6pA015697 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:49:06 -0600 8, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u2AEd2OL014951 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 8, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 21fvmye7km-1 8, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 8, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 8, 35 -- Thu, 10 Mar 2016 08:48:51 -0600 8, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 35 -- Subject: SEM: cleaning used specimen mounts 8, 35 -- Thread-Topic: SEM: cleaning used specimen mounts 8, 35 -- Thread-Index: AdF62+76Cjrsn7dqRqSD4UcP70OFVw== 8, 35 -- Date: Thu, 10 Mar 2016 14:48:49 +0000 8, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F0D084-at-pcfmbx01.wusm-pcf.wustl.edu} 8, 35 -- Accept-Language: en-US 8, 35 -- Content-Language: en-US 8, 35 -- X-MS-Has-Attach: 8, 35 -- X-MS-TNEF-Correlator: 8, 35 -- x-originating-ip: [10.39.135.61] 8, 35 -- Content-Type: text/plain; charset="us-ascii" 8, 35 -- MIME-Version: 1.0 8, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.38,0.0.0000 8, 35 -- definitions=2016-03-10_08:2016-03-10,2016-03-10,1970-01-01 signatures=0 8, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 8, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 8, 35 -- engine=7.0.1-1601100000 definitions=main-1603100240 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u2AEn6pA015697 ==============================End of - Headers==============================
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Email: andykloiber-at-yahoo.com Name: Andrew Kloiber
Organization: Charles River Laboratories - Pathology Associates
Title-Subject: [Filtered] Free TEM, ultramicrotomes,chiller, freezer, cryostat
Message: Hello Listers, We are in need of office space and have some unused equipment that needs to go.
- Zeiss EM 900 in good working condition except for the trackball mechanism is stuck and the stage doesn t move in the Y axis. - Haskris Chiller Model NO. R075S working status unknown. - Revco Ultima II -80 freezer needs compressors. -2 Reichert Austria 0m U2 ultramicrotomes. -Microm HM 505 E Cryostat cutting wheel does not stop.
All of this equipment is available for free pickup at our lab in Durham, NC or you must pay for the shipping and handling. Contact me for more information. andrew.kloiber-at-crl.com
Thanks, Andy
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Email: rms-at-angstrom.us Name: Bob Sommerville
Organization: Angstrom Scientific Inc
Title-Subject: [Filtered] Technical Product Support Specialist Position
Message: Technical Product Support Specialist Eastern Region, US
Angstrom Scientific seeks a Technical Product Support Specialist to provide service and hands-on technical support across our broad portfolio of sophisticated products. This will involve in-house, field, and remote support via both phone and email.
Angstrom Scientific is a sales, service and distribution company for manufacturers of electron microscopes, nano-manipulators, advanced specimen holders, and other scientific (capital) equipment related to nanotech science. We are looking to expand our service support capacity in the eastern US.
Essential Functions / Major Responsibilities: Install, service, maintain, and train customers at their locations on use of our instruments. Provide warranty service and post warranty support (i.e. service contracts) at the customer site, and (via phone and email when appropriate). Ensure all service calls are documented (start to completion) and Service Reports are properly completed and submitted in accordance with established procedures. Maintain own schedule with priorities driven by Service Manager. Provide tradeshow support and ensure demonstration equipment is prepared and fully functional. Assist in organizing, maintaining and tracking inventory (spares, consumables, demo equipment) and take all necessary precautions to safeguard company parts inventory, tools, and equipment.
Specific Job Requirements: Excellent written and verbal communication skills. Strong organizational, problem solving, and analytical skills. Must be detail oriented. Be flexible to needs of a small office environment. Ability to understand and organize technical documentation. Very strong PC and Mac computer skills Clean DMV record Drug testing and background check may be required Approximately 50% travel.
Preferred but not required: Technical degree (at least associates degree or equivalent) Hands-on Technical Field service experience Prior customer interface experience Experience with troubleshooting and diagnostic methods of scientific equipment.
In compliance with federal law, all persons hired will be required to verify identity and eligibility to work in the United States and to complete the required employment eligibility verification document form upon hire
Angstrom Scientific is an equal opportunity employer that does not discriminate on the basis of race, color, national origin, sex, disability, age, religion, sexual orientation, gender identity, gender expression, creed, disabled veteran status, marital status, or Vietnam-era veteran status. Interested Candidates should reply via e-mail to Bob Sommerville at rms -at-angstrom.us
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] Research and Development (R&D) Internships in UK
Message: Role: Two Research and Development (R&D) Internships Location: Abingdon, Oxfordshire, UK Duration: 6 months Internship Description: Gatan is an industry leader in the electron microscopy industry and the R&D Interns will work as part of a multidisciplinary team of scientists, engineers and production staff located in the UK. The roles will provide assistance to technical experts in the delivery of new technology and new product introductions projects. There will be a requirement for collaboration across all business functions working closely with the Manufacturing and Customer Service teams to assure the success of all new product introductions.
The main objective of these internships is learning, personal development, practical application of their scientific/engineering background as well as familiarizing the individuals with the day to day challenges of working in an industrial/commercial environment. The prime responsibility of the R&D Interns will be to engage and take ownership of small projects and product development tasks. Projects may involve sustaining engineering for mature and legacy products.
The product range and technical challenges are diverse and so the ideal candidates will need broad scientific knowledge and must be willing to take on new challenges. The successful candidates will be cable of working semi-autonomously on technical tasks, and will be comfortable working with existing and proven solutions. Training and valuable experience will be provided in the following areas: New product development/introduction (NPD/NPI) procedures and processes The preparation and release of engineering and other technical documentation within the confines of a quality management system (QMS) Engineering change control procedures and product data management (PDM) The roles will report to the Director of Engineering (UK) Some UK and international travel may be required. The following are essential requirements for the internships; At least a bachelor degree in an applied physical science; a higher postgraduate qualification is preferred Fluency in written and oral technical English combined with excellent presentation and general communication skills The ability to work collaboratively and cross-culturally The following skills and experience would be advantageous to the internship; Experience in the construction, operation and application of electron microscopy technology Knowledge of software/firmware programming or a high-level language such MATLAB(R) Knowledge of vacuum technology, cryogenics and thermometry. Optics and light detection Electron optics and electron detection Knowledge of electronics Familiarity with physics modelling software such as COMSOL(R) Familiarity with project management tools and techniques
Interested candidates should submit their CV & covering letter to hrus-at-gatan.com
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The Philadelphia Society for Microscopy will hold its first general meeting of 2016 on Thursday March 10th from 6:30 to 8:30 at Villanova University, Mendel Hall. There will be a short organization meeting followed by a talk by Dr. Robert Carlton (retired) on Pharmaceutical Microscopy. For further information, please visit our Facebook page.
I have a 100x100mm stage in a FEI Quanta ESEM. EDS works - with some problems from Oxygen at 15 Torr with water vapor.
If I did not need to serve a large group of users, I could likely do just fine with powders, particles on 12mm circular stubs or even 7-8mm lengths of curtain rod (Older small SEM's from a very reputable manufacturer) if the specimen was constant in dimensions - as in Quality Control .
Service for us is justified by the patterns of use and the criticality of the work that is done. We have service contracts on both the ESEM and the EDS (this is unusual for an EDS). The reason is that faculty in biology, chemistry and geology with laboratories have included individual student contact with the ESEM/EDS tool as part of laboratory schedule. For the EDS the service contract reduces a loss of detector from an 8-week turnaround to 1.
Considering a SEM to be a tool, a careful list of requirements will likely lead to an easy answer to your question.
Everything about a tool on a bench is about compromise. We have a 250 lb. 'Benchtop' X-Ray Diffractometer with a strip-CCD detector that can provide 60K peaks in a 30min scan over 20-125 degrees 2Theta - perfect for student laboratories. (~$100-150k) There is no service contract even though we use it for student laboratories. In the XRD everything inside is modular: computer, controller and goniometer, source and detector.
We also have a 250 lb. benchtop vacuum coater that can't be put on a bench against a wall, because 75% of its maintenance is done thru the rear of the unit. For our uses - no contract. Cleaning and servicing the TMP is relatively easy. The control system is modular and can easily be diagnosed over the phone and countered by exchange by overnight mail and screw driver and cable changes.
What I have seen of the small SEM with EDS is that data are the same - % by weight and atomic % + a couple others estimates. Currently, my only REAL consideration would be whether the benchtop I purchased had a SDD detector instead of the Si(Li) that all of us users want to replace with one.
Admittedly long, though I hope it helps,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work)
-----Original Message----- X-from: tkremer-at-ipstesting.com [mailto:tkremer-at-ipstesting.com] Sent: Wednesday, February 17, 2016 10:56 AM To: Monson, Frederick {FMonson-at-wcupa.edu}
Hello All,
Do any of you have experience with the newer benchtop or desktop SEM's? They seem quite capable and their cost to purchase and maintain, compared to the full scale SEM's, looks attractive. Their advertised performance is appealing. Do they maintain that performance? Do they require much service? Should I have a service contract? Do the accessories function well (e.g. rotating and tilting stage/holder, charge reduction holder)? Is the provided EDS system comprehensive and reliable? Your replies would be greatly appreciated.
Tom Kremer tkremer-at-ipstesting.com
==============================Original Headers============================== 4, 49 -- From tkremer-at-ipstesting.com Wed Feb 17 09:39:15 2016 4, 49 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0089.outbound.protection.outlook.com [207.46.100.89]) 4, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1HFdF5D011285 4, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Feb 2016 09:39:15 -0600 4, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=ipstesting.com; 4, 49 -- s=selector1; h=From:To:Date:Subject:Message-ID:Content-Type:MIME-Version; 4, 49 -- bh=VdDr4bUQN8m0btp8w4uzLQA45YNYDbgXt2ozzCkUZ2k=; 4, 49 -- b=N33TVBRZ2pA3MFCmJna4eJFnn+LLYBgGmwZa6KTJtq26U7UOIuupjRrcTi3c0TyCI6n3xyIHXpBG0t/Y/Wn/a8kIwuVemVnaMWZSXnS1Ex4clLxTwwuMDI6IWrFdWav8VreiCehu3yZNFj45GkEehKZ9zAi8hL9uPEMgnVOM0N8= 4, 49 -- Received: from BN1PR07MB389.namprd07.prod.outlook.com (10.141.58.141) by 4, 49 -- BN1PR07MB392.namprd07.prod.outlook.com (10.141.58.151) with Microsoft SMTP 4, 49 -- Server (TLS) id 15.1.403.16; Wed, 17 Feb 2016 15:39:00 +0000 4, 49 -- Received: from BN1PR07MB389.namprd07.prod.outlook.com ([169.254.6.115]) by 4, 49 -- BN1PR07MB389.namprd07.prod.outlook.com ([169.254.6.115]) with mapi id 4, 49 -- 15.01.0409.017; Wed, 17 Feb 2016 15:38:59 +0000 4, 49 -- From: Tom Kremer {tkremer-at-ipstesting.com} 4, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 49 -- Subject: SEM with EDS Comparing the new benchtop desktop instruments 4, 49 -- Thread-Topic: SEM with EDS Comparing the new benchtop desktop instruments 4, 49 -- Thread-Index: AdFpmVC8JLl6k7olThyW8RmBOWZLAw== 4, 49 -- Date: Wed, 17 Feb 2016 15:38:59 +0000 4, 49 -- Message-ID: {BN1PR07MB389AA742ACB826C23947175DBAE0-at-BN1PR07MB389.namprd07.prod.outlook.com} 4, 49 -- Accept-Language: en-US 4, 49 -- Content-Language: en-US 4, 49 -- X-MS-Has-Attach: 4, 49 -- X-MS-TNEF-Correlator: 4, 49 -- authentication-results: microscopy.com; dkim=none (message not signed) 4, 49 -- header.d=none;microscopy.com; dmarc=none action=none 4, 49 -- header.from=ipstesting.com; 4, 49 -- x-originating-ip: [96.11.14.178] 4, 49 -- x-microsoft-exchange-diagnostics: 1;BN1PR07MB392;5:G8+PY0J2GNNl8YzY/4J/6m/QzJ+lvK79Zudi6lHgOY8LMKDPViODaTPuhu9MCE/kfe5l2R7WX0OWH+t5rEfNkUll3P07lfJxfBBcst10ZNIcPQk24Qnt1Uooq/Sx+nouq/bSkrw6AskKMP5abIuw9w==;24:YTPJyYYh4Aeab+DeGjHe/ZXeQAnJjxjtmFbcsq8KuFmdQR5JLPVgVbJ2DmplFbhuH27/WQ4/8bhlp8O7id2GbyY07mulSdPROQZ49W4Qm40= 4, 49 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BN1PR07MB392; 4, 49 -- x-ms-office365-filtering-correlation-id: ce3d9565-3cf6-4da1-785c-08d337b0748a 4, 49 -- x-microsoft-antispam-prvs: {BN1PR07MB392B77C4215F666C8D74AE3DBAE0-at-BN1PR07MB392.namprd07.prod.outlook.com} 4, 49 -- x-exchange-antispam-report-test: UriScan:; 4, 49 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046);SRVR:BN1PR07MB392;BCL:0;PCL:0;RULEID:;SRVR:BN1PR07MB392; 4, 49 -- x-forefront-prvs: 085551F5A8 4, 49 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(53754006)(122556002)(19580405001)(5002640100001)(2900100001)(92566002)(3280700002)(66066001)(33656002)(99286002)(5003600100002)(19580395003)(2906002)(3660700001)(5004730100002)(5008740100001)(229853001)(450100001)(2351001)(10400500002)(1096002)(189998001)(54356999)(50986999)(6116002)(102836003)(1220700001)(87936001)(110136002)(3846002)(40100003)(86362001)(74316001)(107886002)(5001960100002)(76576001)(586003)(2501003);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR07MB392;H:BN1PR07MB389.namprd07.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 4, 49 -- spamdiagnosticoutput: 1:23 4, 49 -- spamdiagnosticmetadata: NSPM 4, 49 -- Content-Type: text/plain; charset="us-ascii" 4, 49 -- MIME-Version: 1.0 4, 49 -- X-OriginatorOrg: ipstesting.com 4, 49 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 17 Feb 2016 15:38:59.4631 4, 49 -- (UTC) 4, 49 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 49 -- X-MS-Exchange-CrossTenant-id: fcd656df-08de-421e-a3c5-e640523dfad4 4, 49 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR07MB392 4, 49 -- Content-Transfer-Encoding: 8bit 4, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1HFdF5D011285 ==============================End of - Headers==============================
You been one of the pillars of the community. We are sorry to see you go and hope to still see you occasionally post a wise word or two.
Enjoy retirement!
Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com
Microscopy/Microscopy Education is a division of The Microscopy & Imaging Place, Inc.
NEW! Getting involved in Raman or FTIR? MME is now offering courses in these areas specifically for microscopists! Now scheduling courses through the mid 2016. We can customize a course on nearly any topic, from fluorescence to confocal to image analysis to SEM/TEM. Call today for a free training evaluation.
At 05:44 PM 2/29/2016, you wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Tomorrow is my last day at Arizona State University. I am retiring after 44 years of microscopy. Well, we all know that electron microscopes are pretty cool, so my work years have been fun, and all the people around the microscopes are of a similar mind-set, so that has made the work cool also. Thanks to Professor Will Bigelow and Dr. Larry Allard at the University of Michigan for introducing me to this world and training me to do well in it, thanks to Professor Ray Carpenter for recruiting me to ASU, and thanks to Nestor Zaluzec for maintaining the Listserver for all these years. Well, I must have been fated to work in the field, because in the year I was born:
. JEOL was founded as the Japan Electron Optics Laboratory Company, Limited, Tokyo. . Philips First production Transmission Electron Microscope (TEM) system was released . Raimond Castaing showed the first functioning microprobe. . C.W. Oatley built an SEM based on Zworykin's microscope. . First microscopic analysis of thin metal foils and viewing dislocations was accomplished by Robert Heidenreich.
For anyone else who I did not mention explicitly (and there are too many to list), thanks also.
Nestor, do not disconnect me, because retirees from this institution retain their email accounts, so even though I won't be battling traffic to get to the lab, I will still read postings.
A. John Mardinly, Ph.D., P.E. Principal Materials Nanoanalysis Engineer EMail: John.Mardinly-at-ASU.edu John Cowley Center for HREM, LE-CSSS B134B Bateman Physical Sciences Building Arizona State University PO Box 871704 Tempe, AZ 85287-1704
==============================Original Headers============================== 7, 37 -- From John.Mardinly-at-asu.edu Mon Feb 29 17:24:16 2016 7, 37 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1TNOGRO023534 7, 37 -- for {Microscopy-at-Microscopy.com} ; Mon, 29 Feb 2016 17:24:16 -0600 7, 37 -- X-ASG-Debug-ID: 1456788215-06a86775a812dc0004-FOsErg 7, 37 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnet8.asu.edu with ESMTP id oeRCKbgQ6wYTjRuR (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Mon, 29 Feb 2016 16:23:59 -0700 (MST) 7, 37 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 7, 37 -- X-Barracuda-Effective-Source-IP: exhubw01.asurite.ad.asu.edu[129.219.4.199] 7, 37 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 7, 37 -- X-ASG-Whitelist: Client 7, 37 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 7, 37 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.03.0266.001; 7, 37 -- Mon, 29 Feb 2016 16:23:53 -0700 7, 37 -- From: John Mardinly {John.Mardinly-at-asu.edu} 7, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 7, 37 -- Subject: Retirement 7, 37 -- Thread-Topic: Retirement 7, 37 -- X-ASG-Orig-Subj: Retirement 7, 37 -- Thread-Index: AdFzSEAryoJsdNYZQGW4p53E2iOQ6Q== 7, 37 -- Date: Mon, 29 Feb 2016 23:23:52 +0000 7, 37 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE940248FEBA0-at-exmbt01.asurite.ad.asu.edu} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [129.219.103.4] 7, 37 -- Content-Type: text/plain; charset="iso-8859-1" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] 7, 37 -- X-Barracuda-Start-Time: 1456788239 7, 37 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 7, 37 -- X-Barracuda-URL: https://129.219.117.210:443/cgi-mod/mark.cgi 7, 37 -- X-Barracuda-Scan-Msg-Size: 1627 7, 37 -- X-Virus-Scanned: by bsmtpd at asu.edu 7, 37 -- X-Barracuda-BRTS-Status: 1 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1TNOGRO023534 ==============================End of - Headers==============================
Tomorrow is my last day at Arizona State University. I am retiring after 44 years of microscopy. Well, we all know that electron microscopes are pretty cool, so my work years have been fun, and all the people around the microscopes are of a similar mind-set, so that has made the work cool also. Thanks to Professor Will Bigelow and Dr. Larry Allard at the University of Michigan for introducing me to this world and training me to do well in it, thanks to Professor Ray Carpenter for recruiting me to ASU, and thanks to Nestor Zaluzec for maintaining the Listserver for all these years. Well, I must have been fated to work in the field, because in the year I was born:
. JEOL was founded as the Japan Electron Optics Laboratory Company, Limited, Tokyo. . Philips First production Transmission Electron Microscope (TEM) system was released . Raimond Castaing showed the first functioning microprobe. . C.W. Oatley built an SEM based on Zworykin's microscope. . First microscopic analysis of thin metal foils and viewing dislocations was accomplished by Robert Heidenreich.
For anyone else who I did not mention explicitly (and there are too many to list), thanks also.
Nestor, do not disconnect me, because retirees from this institution retain their email accounts, so even though I won't be battling traffic to get to the lab, I will still read postings.
A. John Mardinly, Ph.D., P.E. Principal Materials Nanoanalysis Engineer EMail: John.Mardinly-at-ASU.edu John Cowley Center for HREM, LE-CSSS B134B Bateman Physical Sciences Building Arizona State University PO Box 871704 Tempe, AZ 85287-1704
==============================Original Headers============================== 7, 37 -- From John.Mardinly-at-asu.edu Mon Feb 29 17:24:16 2016 7, 37 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1TNOGRO023534 7, 37 -- for {Microscopy-at-Microscopy.com} ; Mon, 29 Feb 2016 17:24:16 -0600 7, 37 -- X-ASG-Debug-ID: 1456788215-06a86775a812dc0004-FOsErg 7, 37 -- Received: from exhubw01.asurite.ad.asu.edu (exhubw01.asurite.ad.asu.edu [129.219.4.199]) by bcnet8.asu.edu with ESMTP id oeRCKbgQ6wYTjRuR (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Mon, 29 Feb 2016 16:23:59 -0700 (MST) 7, 37 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 7, 37 -- X-Barracuda-Effective-Source-IP: exhubw01.asurite.ad.asu.edu[129.219.4.199] 7, 37 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 7, 37 -- X-ASG-Whitelist: Client 7, 37 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 7, 37 -- exhubw01.asurite.ad.asu.edu ([129.219.4.199]) with mapi id 14.03.0266.001; 7, 37 -- Mon, 29 Feb 2016 16:23:53 -0700 7, 37 -- From: John Mardinly {John.Mardinly-at-asu.edu} 7, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 7, 37 -- Subject: Retirement 7, 37 -- Thread-Topic: Retirement 7, 37 -- X-ASG-Orig-Subj: Retirement 7, 37 -- Thread-Index: AdFzSEAryoJsdNYZQGW4p53E2iOQ6Q== 7, 37 -- Date: Mon, 29 Feb 2016 23:23:52 +0000 7, 37 -- Message-ID: {4FFC63ED830B1C41BACD3ABA182BE940248FEBA0-at-exmbt01.asurite.ad.asu.edu} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [129.219.103.4] 7, 37 -- Content-Type: text/plain; charset="iso-8859-1" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-Barracuda-Connect: exhubw01.asurite.ad.asu.edu[129.219.4.199] 7, 37 -- X-Barracuda-Start-Time: 1456788239 7, 37 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 7, 37 -- X-Barracuda-URL: https://129.219.117.210:443/cgi-mod/mark.cgi 7, 37 -- X-Barracuda-Scan-Msg-Size: 1627 7, 37 -- X-Virus-Scanned: by bsmtpd at asu.edu 7, 37 -- X-Barracuda-BRTS-Status: 1 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1TNOGRO023534 ==============================End of - Headers==============================
Thanks to the heads up from Lucille Giannuzzi a problem with the Listserver archives which was inadvertently created is now fixed.
Basically the problem was that messages were not being stored due to a mistake on my part which affected all of March, April and the the first 2 weeks of May.
The archives are now restored/fixed and the missing messages have been reinserted into the archive file. The only glitch is that when I inserted the missing messages into the archive, the temporal order is backwards for the restored messages.
This means if you happen to scan/dump the archives you will see messages in the temporal sequential order of: Jan, Feb, May(1-12), April, March, then back to May (13-} ).
Fixing the exact order is alot of work as I would have to manually cut and paste messages into the correct order. I'll put that off for now.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
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==============================Original Headers============================== 11, 49 -- From microscopy.listserver-at-gmail.com Thu May 12 20:15:54 2016 11, 49 -- Received: from mail-io0-f181.google.com (mail-io0-f181.google.com [209.85.223.181]) 11, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4D1Fs5Z031856 11, 49 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 20:15:54 -0500 11, 49 -- Received: by mail-io0-f181.google.com with SMTP id d62so115513626iof.2 11, 49 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 18:16:08 -0700 (PDT) 11, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 49 -- d=gmail.com; s=20120113; 11, 49 -- h=from:to:reply-to:subject:message-id:date:user-agent:mime-version 11, 49 -- :content-transfer-encoding; 11, 49 -- bh=lEiJRwd74Nqgo0L7kEvdVUVbQxLZg9SwTm5WjTNbO/0=; 11, 49 -- b=reovxdVZur6Y/UKoQWAPxfzcdf9Ka79vp+FX2U46Hqp51jfyfEAD1e6PJtcCTPV93v 11, 49 -- B8p5+Ttr0ngR2ekfLt2aKgXqWzR2AGeDKeToRUcruwTCL90YhoqweApczTlD4j9UaFXN 11, 49 -- I+k6Ib/0SGdD7xnDep39MSxqNX3X4icthWSRGX5J26P/xgn+d6l9DOEFW5ZjywJGWJoN 11, 49 -- JX9pgjdrxCVv8/uNR4bUCSOX8FYrbWxRnAQgtG/5TQMHOmLdsSOi2A6rmykCIwx6X4te 11, 49 -- Zp11JtlTSvts7hNE9mgmRCLyi5LwFfdrwTWT3CuzU+a5J3Ab4kXpIax+dAuNJ5UzTUA8 11, 49 -- QAog== 11, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 49 -- d=1e100.net; s=20130820; 11, 49 -- h=x-gm-message-state:from:to:reply-to:subject:message-id:date 11, 49 -- :user-agent:mime-version:content-transfer-encoding; 11, 49 -- bh=lEiJRwd74Nqgo0L7kEvdVUVbQxLZg9SwTm5WjTNbO/0=; 11, 49 -- b=UUEg8C5lhPhrPtC0sOpISXxrJDXFnhrCmh1wHaiWIITxYEBUySDIbIZJ0w0bmQZeEk 11, 49 -- NCTe1h7myyp6gxCKxBOyUTHW5lSe7o4plr6A1qN2kV2eDPEqhhUK+P3cIYhHdXSW+enz 11, 49 -- rg1hXd7oCYTl6h8G3gEiY7WoDDSmoUReG6In47jerfzNGU6VYTqBPkX90OTm3RpvwVbh 11, 49 -- ZWiktzcNz9Sq3WReAR4zBeLiKLnubYGynZPe1mxBPpLZTP4mLrtObmcJDdf5wEaSTjz+ 11, 49 -- UiN2Koz3rYRs/g7U72D1R8TTO2xhRu3KH8EjJk46OtOi2yyrw5G0n/YQRK0foI7Um1vG 11, 49 -- /PNw== 11, 49 -- X-Gm-Message-State: AOPr4FXZLizwfmO8Qo98vulsV0yHeFhVQgYIRW04sQvEQ1f5xmMXO7PmKXSOL+L/Rmqvfw== 11, 49 -- X-Received: by 10.36.139.71 with SMTP id g68mr157826ite.68.1463102168254; 11, 49 -- Thu, 12 May 2016 18:16:08 -0700 (PDT) 11, 49 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 11, 49 -- by smtp.googlemail.com with ESMTPSA id 198sm256648itk.5.2016.05.12.18.16.07 11, 49 -- for {microscopy-at-microscopy.com} 11, 49 -- (version=TLSv1/SSLv3 cipher=OTHER); 11, 49 -- Thu, 12 May 2016 18:16:07 -0700 (PDT) 11, 49 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 11, 49 -- X-Google-Original-From: MicroscopyListserver-NoReply 11, 49 -- {microscopylistserver-noreply-at-microscopy.com} 11, 49 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 49 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 11, 49 -- Subject: Administrivia: Listserver Archives back working 11, 49 -- Message-ID: {2087efde-4c1e-c31c-ccc6-2f1b07b530f7-at-microscopy.com} 11, 49 -- Date: Thu, 12 May 2016 20:16:07 -0500 11, 49 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 11, 49 -- Gecko/20100101 Thunderbird/45.0 11, 49 -- MIME-Version: 1.0 11, 49 -- Content-Type: text/plain; charset=utf-8; format=flowed 11, 49 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Have any of you checked the resolution of your EDS detector to compare it's resolution with what you bought versus what you have?
I discovered that the Mn FWHM is a known standard method, but the EDS makers don't necessarily actually use this. It seems that they use a radioactive Fe specimen and then correlate that to Mn. I'm wondering if the correlation/translation from Fe to Mn is valid. My current system is not.
The situation could be that you bought a 123eV detector but wound up with one that is greater than 123eV. The delivered spec might say {=123eV yet the resolution is actually worse than this.
Any feedback or comments?
gary g.
==============================Original Headers============================== 6, 32 -- From gary-at-gaugler.com Thu May 12 23:10:30 2016 6, 32 -- Received: from wavecable.net (cgfe-33-tuk.wavecable.net [66.235.59.136]) 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4D4AUrX023584 6, 32 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 23:10:30 -0500 6, 32 -- Received: from [172.16.141.14] (HELO wavecable.net) 6, 32 -- by cgfe-3-tuk.wavecable.net (CommuniGate Pro SMTP 6.1.8) 6, 32 -- with ESMTP id 5240642 for microscopy-at-microscopy.com; Thu, 12 May 2016 21:10:44 -0700 6, 32 -- X-Junk-Score: 0 [] 6, 32 -- X-Cloudmark-Score: 0 [] 6, 32 -- X-Cloudmark-Analysis: v=2.1 cv=YNxiskyx c=1 sm=1 tr=0 a=xcX17FhCtwk1rQrrxiCS2g==:117 a=IkcTkHD0fZMA:10 a=helqL4AUOeMA:10 a=SEOrLOue2igA:10 a=yrkiwgmsf1kA:10 a=v6QcmHujqooODp5vm94A:9 a=QEXdDO2ut3YA:10 6, 32 -- Received: from [172.16.141.12] (HELO wavecable.net) 6, 32 -- by cgfe-4-tuk.wavecable.net (CommuniGate Pro SMTP 6.1.8) 6, 32 -- with ESMTP id 1149616 for microscopy-at-microscopy.com; Thu, 12 May 2016 21:10:44 -0700 6, 32 -- X-Junk-Score: 0 [] 6, 32 -- X-Cloudmark-Score: 0 [] 6, 32 -- X-Cloudmark-Analysis: v=2.1 cv=U5pvdrfu c=1 sm=1 tr=0 a=2VnlTcbn8LW/DKys2mm+zQ==:117 a=IkcTkHD0fZMA:10 a=SEOrLOue2igA:10 a=yrkiwgmsf1kA:10 a=v6QcmHujqooODp5vm94A:9 a=QEXdDO2ut3YA:10 6, 32 -- Received: from [192.92.0.19] (HELO [127.0.0.1]) 6, 32 -- by wavecable.com (CommuniGate Pro SMTP 6.1.8) 6, 32 -- with ESMTP id 2580244 for microscopy-at-microscopy.com; Thu, 12 May 2016 21:10:43 -0700 6, 32 -- Reply-To: gary-at-gaugler.com 6, 32 -- To: microscopy-at-microscopy.com 6, 32 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 32 -- Subject: EDS detector resolution checking 6, 32 -- Message-ID: {573553BE.1080201-at-gaugler.com} 6, 32 -- Date: Thu, 12 May 2016 21:10:38 -0700 6, 32 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 6, 32 -- Thunderbird/38.3.0 6, 32 -- MIME-Version: 1.0 6, 32 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 32 -- Content-Transfer-Encoding: 7bit 6, 32 -- X-Antivirus: avast! (VPS 160512-1, 05/12/2016), Outbound message 6, 32 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
I bought a small standard block from Ted Pella that has Mn as one of the standards. It also has a BN standard useful for checking low end performance. I record a spectrum from the standard using my standard conditions, save it in MSA format, and then use the NIST-DTSA-II to process the spectrum. One first needs to configure a DTSA detector and then run the detector calibration “alien". It will calibrate from the Mn. The routine automatically computes the resolution.
It turns out that one can do a pretty good job with a piece of Cu tape or a Cu TEM grid mounted on an SEM stub. If one measures the Cu spectrum at 25 kV the intensity of the Cu K and L lines are pretty close. One can then run the detector calibration from the Cu spectrum. The results on our system are within experimental error (which DTSA automatically computes!) of the results from Mn. What else would you expect from Nicholas Ritchie and his colleagues at NIST!
I have no commercial interest in Ted Pella; just a long time satisfied customer. Also a grateful user of DTSA-II.
Best regards, John Minter Kodak Analytical Sciences
==============================Original Headers============================== 4, 46 -- From jrminter-at-gmail.com Fri May 13 17:44:22 2016 4, 46 -- Received: from mail-qg0-f47.google.com (mail-qg0-f47.google.com [209.85.192.47]) 4, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4DMiMKo003163 4, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2016 17:44:22 -0500 4, 46 -- Received: by mail-qg0-f47.google.com with SMTP id w36so65360941qge.3 4, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2016 15:44:40 -0700 (PDT) 4, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 46 -- d=gmail.com; s=20120113; 4, 46 -- h=from:content-transfer-encoding:subject:date:message-id:cc:to 4, 46 -- :mime-version; 4, 46 -- bh=bDsr+fo1gRr5MbtbPFFqe5rGPCO+PMorDYIUddt/PWc=; 4, 46 -- b=0Si4yAukNz3P/CjaaK8Krq7UNnDy/Nm/L+zucJ34ld8jsq/C6NuCDvyIPEWME2WrIj 4, 46 -- oaze8zYJRhRZR2vkVbCZVaP9ZHNRhXUEWm3XagaQVxyt8rxTPHl8YU6V6wGdp30nMxDd 4, 46 -- soq4YCFTuSQyuVfqjcmrqy2K4+8S8iGJ7qBC/UdHE8HonqUfAxbPKwBYaHTKW6Gs2beE 4, 46 -- 52bDhGD4OY1fY1n6kMKReiG+JQ6bJ+JZw4ps1arWJIwn6Mi44ngJ4q03Vh7am+Wp5QNC 4, 46 -- 2FLGqOp3Hpuj2yAXXg0+0FYqsgok+zj1mVJVhN6JOF27XoGq6S9ABuhduHx9GFomfOFF 4, 46 -- hFiw== 4, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 46 -- d=1e100.net; s=20130820; 4, 46 -- h=x-gm-message-state:from:content-transfer-encoding:subject:date 4, 46 -- :message-id:cc:to:mime-version; 4, 46 -- bh=bDsr+fo1gRr5MbtbPFFqe5rGPCO+PMorDYIUddt/PWc=; 4, 46 -- b=fyijPyExu0zoZFk8gY+f1FgQ87WZBV3/sLQ+62YMULkAwb+1gkD8gjDGKuS4xbT3O3 4, 46 -- 1e/f7KeWfysRgf7OmOo2YtDQqavValOg0GZuFZywgP0boC2X1qcTp8v9nWPTb7G7bWsS 4, 46 -- sxzzm8F2RFTRnDgS9GOWX69/H5D4kIMbDnen4enxphLOXas5U2zRDYIGeAQJZH3udSx+ 4, 46 -- gTGGjrf6c3zXoxvmMFFjlP1Imgczz3Qvsm2T3FaeL/3jYpdcgICRuBV5z3D7/nGvEoE9 4, 46 -- LH49nQYCB2faOmRFAyIlY0G1ZnGQYnuLLzwXoBLo3Y+4JRl61hL93pMmwyWcrJCRbyM3 4, 46 -- WR0g== 4, 46 -- X-Gm-Message-State: AOPr4FWRedcDqzp7Don23Es3/DJjxT+2UrYdIxbYwL1S+St9CHAuvFN9+uBZeSdE2FXnPQ== 4, 46 -- X-Received: by 10.140.101.37 with SMTP id t34mr18172036qge.57.1463179479948; 4, 46 -- Fri, 13 May 2016 15:44:39 -0700 (PDT) 4, 46 -- Received: from ?IPv6:2604:6000:1000:9a:8802:ccda:50e9:3af3? ([2604:6000:1000:9a:8802:ccda:50e9:3af3]) 4, 46 -- by smtp.gmail.com with ESMTPSA id 85sm9272161qhx.38.2016.05.13.15.44.38 4, 46 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 4, 46 -- Fri, 13 May 2016 15:44:39 -0700 (PDT) 4, 46 -- From: John Minter {jrminter-at-gmail.com} 4, 46 -- Content-Type: text/plain; charset=utf-8 4, 46 -- Subject: Re: EDS Detector Resolution 4, 46 -- Date: Fri, 13 May 2016 18:44:38 -0400 4, 46 -- Message-Id: {089E06CE-E002-4204-B89C-1260BDB0E4E7-at-gmail.com} 4, 46 -- Cc: gary-at-gaugler.com 4, 46 -- To: Microscopy-at-microscopy.com 4, 46 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 4, 46 -- X-Mailer: Apple Mail (2.3124) 4, 46 -- Content-Transfer-Encoding: 8bit 4, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4DMiMKo003163 ==============================End of - Headers==============================
} Have any of you checked the resolution of your EDS detector } to compare it's resolution with what you bought versus what } you have? } } I discovered that the Mn FWHM is a known standard method, but } the EDS makers don't necessarily actually use this. It seems } that they use a radioactive Fe specimen and then correlate } that to Mn. I'm wondering if the correlation/translation from } Fe to Mn is valid. My current system is not. } } The situation could be that you bought a 123eV detector but } wound up with one that is greater than 123eV. The delivered } spec might say {=123eV yet the resolution is actually worse } than this. }
Dear Gary --
I'm one of the guilty parties, going back about 4 decades.
First, the emission of an Fe55 radioactive source really is the Mn K lines. It is identical to the K emission of Mn under the beam, except there is no Brehmsstrahlung background. Instead of ionization by an electron beam, the excited Mn is created by electron capture from an Fe55 nucleus (nuclear proton grabs an electron and becomes a neutron, reducing the atomic number by 1). It is convenient and very safe, because no other radiation is emitted (*). Sources up to 100 uCuries can be sent by regular mail with no special packaging.
Detectors are normally tested in a controlled environment "on the bench" with a source. There are many reasons a detector might not meet spec on a column in the real world besides the detector itself. For example, CE marking be d*mned, we've discovered that one of our Tektronix digital oscilloscopes costs about 1.5 eV if it's anywhere near the BNC cables from the detector to the pulse processor. X-ray detectors are exquisitely sensitive EMI detectors too. You might find for that resolution varies slightly with the time of day, depending on what other equipment in the area is operating. I've spent many happy (?) hours at various installations trying to figure out what was causing resolution degradation. 123 eV is close to the absolute limit for a silicon-based detector, and it only takes microvolts of noise to kick that up an eV or so.
Anybody else have enough gray in their hair to remember when anything better than 150 eV was a pretty decent detector?
I second the kudos to Nicholas Ritchie! DTSA-II is an extraordinarily useful tool for all aspects of EDS analysis. Good luck figuring out what's going on.
Rick Mott, PulseTor LLC (formerly with PGT back when woolly mammoths still walked the hills of New Jersey)
(*) Ok, all you picky nuclear physicists out there. There are extremely low probability gammas up to 231 KeV, 5 to 7 orders of magnitude lower in intensity than the Mn K lines. Not worth losing sleep over.
==============================Original Headers============================== 13, 20 -- From rmott-at-pulsetor.com Sat May 14 11:19:38 2016 13, 20 -- Received: from p3plwbeout14-01.prod.phx3.secureserver.net (p3plsmtp14-01-2.prod.phx3.secureserver.net [173.201.192.182]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4EGJcI2030124 13, 20 -- for {microscopy-at-microscopy.com} ; Sat, 14 May 2016 11:19:38 -0500 13, 20 -- Received: from localhost ([173.201.192.150]) 13, 20 -- by p3plwbeout14-01.prod.phx3.secureserver.net with bizsmtp 13, 20 -- id uFBo1s0013F9LFh01FBoiM; Sat, 14 May 2016 08:11:48 -0700 13, 20 -- X-SID: uFBo1s0013F9LFh01 13, 20 -- Received: (qmail 28691 invoked by uid 99); 14 May 2016 15:11:48 -0000 13, 20 -- Content-Type: text/plain; charset="utf-8" 13, 20 -- X-Originating-IP: 50.182.112.87 13, 20 -- User-Agent: Workspace Webmail 6.2.9 13, 20 -- Message-Id: {20160514081147.26bcc40673c3b2d777d54ed9d517fad5.929a003db3.wbe-at-email14.godaddy.com} 13, 20 -- From: "Rick Mott" {rmott-at-pulsetor.com} 13, 20 -- To: microscopy-at-microscopy.com 13, 20 -- Subject: Re: [Microscopy] EDS detector resolution checking 13, 20 -- Date: Sat, 14 May 2016 08:11:47 -0700 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4EGJcI2030124 ==============================End of - Headers==============================
Dear Listservers it seems we're in big troubles with the EDS of our TEM. We have an old Oxford ISIS Series 300, and it isn't collecting signal any longer. It seems it's the XAC card, which has been discontinued by Oxford, unfortunately. I fear that we won't be able to gather the money for a new system, so it would be great if some of you had an old ISIS - or just the XAC card - no longer in use, to give us. Thanks
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Email: baxau-at-mail.nih.gov Name: Ulrich Baxa
Organization: Frederick National Laboratory for Cancer Research
Title-Subject: [Filtered] Scientist position
Message: Dear Colleagues,
The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Scientist I. Main duties will include: 1) management of a small user facility with sample processing lab and FEI T12 microscope, 2) biological sample preparation for electron microscopy (traditional plastic embedding, high pressure freezing/freeze substitution, CLEM, other approaches as necessary), 3) help users with routine imaging and training of new users. If interested, please apply directly using the following link:
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We would like to draw your attention to the upcoming Yale Microscopy Workshop, a 2-day event that offers open access to a wide variety of microscope.
Yale Microscopy Workshop June 14-15th, 2016
This year's symposia theme is “Novel Fluorescent Probes and Labeling Techniques". We will also offer a small group practical micro-course on “Spectral detection and spectra unmixing". As usual, there are a very broad range of technical lectures and demonstrations that are too numerous to mention here, but include a practical lecture on sample preparation for Correlative Light and EM. There will also be vendor presentations on commercially available probes to highlight cell structures or function.
Because this year’s symposia topic is focused on probes and labeling, there will not be any additional microscopes installed by vendors. Instead we will continue to take advantage of some of the excellent microscopes in facilities on campus, including two Leica confocals with spectral detectors in the CCMI facility, one of them with STED superresolution capabilities.
Something for everyone, for beginners as well as the advanced microscopists! Please see the website for a complete listing of events: http://medicine.yale.edu/lab/microscopy/schedule.
Organizers: Ann Haberman Derek Toomre Joerg Bewersdorf Xinran Liu
With this year's spring cleaning and microscope consolidation, I've ended up with a what I believe to be a brand new (or nearly so; I can't see any flaws) 63X/1.25 Zeiss Plan-Neofluar oil immersion objective. I more or less have these coming out of my ears, but am short a 20X objective. If anybody has a spare and gently used 20X/0.50 Zeiss Plan-Neofluar I'd happily lose magnification, numerical aperture and capital value in a straight swap.
Contact me off-list if interested.
Thanks, Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
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Email: michael.pidgeon-at-uhsystem.com Name: Michael Pidgeon
Organization: University Health / Louisiana State University
Title-Subject: [Filtered] TEM service
Message: Does any know of an independent TEM service rep or tech that offers service contracts?
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Email: skmeno1-at-nps.edu Name: SARATH MENON
Organization: NAVAL POSTGRADUATE SCHOOL
Title-Subject: [Filtered] Tenure Track Position at Naval Postgraduate School
Message: TENURE-TRACK ASSISTANT PROFESSOR Material Science The department of Mechanical and Aerospace Engineering (MAE) at the Naval Postgraduate School, in Monterey California, is seeking outstanding candidates for a tenure-track Assistant Professor position starting September 2016. The candidate should have a Ph.D. in Mechanical Engineering or in Material Science/Engineering. Demonstrated expertise in structural materials and advance materials characterization is required, preferentially with an emphasis on metal joining and corrosion. New faculty are expected to develop high quality externally-funded research programs and teach at the graduate levels. The candidate will have an exciting opportunity to collaborate with faculty in the Center for Materials Research that includes the Departments of MAE, Physics and Electrical and Computer Engineering. Facilities within MAE include a new Transmission Electron Microscope, a Scanning Electron Microscope, as well as a new Metallic Cold Spray facility, among others. Review of applicants will begin April 1, 2016. The Naval Postgraduate School is committed to enhancing the diversity of its faculty and staff and encourages applications from women, minorities, people with disabilities and veterans. As a tenure-track faculty member the applicant must be able to obtain a security clearance. The Naval Postgraduate School offers a competitive salary, start-up package, and benefits. Please visit our department website at http://www.nps.edu/Academics/GSEAS/MAE/
Garth V. Hobson Professor and Chair Department of Mechanical & Aerospace Engineering 700 Dyer Road, Bldg 245 Watkins Hall, WA-338 Naval Postgraduate School Monterey, CA 93943 TEL: 831-656-2586/831-656-2888 FAX: 831-656-2238 www.nps.edu/mae gvhobson-at-nps.edu
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Michael, I have info on an independent contratctor (Remi) that will service microscopes. We have not used him, but here is the contact email:
X-from: Tim Johnson [mailto:Tim.Johnson-at-theremigroup.com] Sent: Wednesday, April 06, 2016 9:26 AM To: 'mdelann1-at-jhmi.edu'
X-from: michael.pidgeon-at-uhsystem.com
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Email: michael.pidgeon-at-uhsystem.com Name: Michael Pidgeon
Organization: University Health / Louisiana State University
Title-Subject: [Filtered] TEM service
Message: Does any know of an independent TEM service rep or tech that offers service contracts?
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Message: Hi all, I need recommendations for a basic light microscope with a camera system. Let me know if you have a system in your lab that you love (Leica, Zeiss, Olympus or another company). thanks, Beth Richardson Georgia Electron Microscopy Lab University of Georgia Athens, GA 30602 USA
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Email: rgmorse-at-morse-associates.com Name: Roger G Morse
Organization: Morse Associates
Title-Subject: [Filtered] SEM free to good home
Message: I have an old (1970's vintage) Cambridge Stereoscan 600 that I would like to give to somebody who could use it. It last worked about 12 years ago, and is complete. I expect it could be very useful for parts for anybody that is trying to keep an old Stereoscan working.
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Dear All, can anybody help me with an operation manual and a manual documenting the API of the remote control on the Jeol 6500 FESEM in PDF?
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Title-Subject: [Filtered] Tannic acid staining for microtubule in brain
Message: Dear list,
I am experiencing sub-optimal microtubule staining in ependymal cells in mouse brain tissue by tannic acid, while the same protocol works fine on microtubules in cultured cells. I use 0.1% of tannic acid in 2.5% glutaraldehyde for 1 hour. I extended the incubation time to overnight for the brain experiments but it was still selective and weak. Any advice is appreciated.
Regards, Hiro ----------- Kunihiro Uryu, Ph.D., Research Assistant Professor Director of Electron Microscopy Resource Center (EMRC)
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Email: ypicard-at-cmu.edu Name: Yoosuf Picard
Organization: Carnegie Mellon University
Title-Subject: [Filtered] M&M 2016 Pre-Meeting Congress - TEM and SEM Diffraction Workshop
Message: EVENT: Pre-Meeting Congress on Exploiting the Diffractive Properties of Electrons for Solving Materials Problems
http://www.microscopy.org/MandM/2016/program/congress.cfm DATE: Sunday, July 24, 2016 preceding the Microscopy and Microanalysis 2016 meeting
LOCATION: Columbus, Ohio, USA at M&M 2016
1 Day Workshop: 8 In-Depth Tutorials featuring Practical Advice and Surveying Various Case Studies / Latest Research Highlights on SEM and TEM Diffraction-Based Methods
REGISTRATION: $149 for MAS/MSA/IMS members; $50 for students Link to register for M&M 2016 and this event: https://ww2.eventrebels.com/er/Registration/StepRegInfo.jsp?ActivityID=15715&StepNumber=1
Link if youve already registered for M&M 2016: https://ww2.eventrebels.com/er/Registration/UpdateInfo.jsp?ActivityID=15715
PROGRAM: Marc De Graef, Carnegie Mellon University - Introduction to crystallography and electron diffraction
John Mansfield, University of Michigan - Conventional TEM Diffraction Methods for Materials Analysis and Crystallography
Joe Michael, Sandia National Laboratories - Introduction and Survey of Electron Backscattered Diffraction
Ute Kolb, University of Mainz - Precession Diffraction for TEM-based Orientation Mapping
Ben Britton, Imperial College - Strain Mapping by HR-EBSD
PURPOSE OF EVENT: Diffraction is a fundamental mechanism for structural characterization of materials by both transmission electron microscopes (TEMs) and scanning electron microscopes (SEMs). For new users, diffraction methods in TEM and SEM are often taught in separate courses or workshops even though the underlying physics and materials applications overlap substantially. Also, there rarely exist educational opportunities that singularly cover the myriad options for TEM/SEM based characterization of crystal structure, orientation, strain and defects where electron diffraction is central.
To address this concern, a Pre-Meeting Congress entitled Exploiting the Diffractive Properties of Electrons for Solving Materials Problems will be held Sunday, July 24, 2016 in Columbus, OH, USA. This one-day meeting is being organized by us, the Electron Crystallography & Automated Mapping Techniques (ECAMT) Focused Interest Group (FIG), within the purview of the Microscopy Society of America (MSA). This one day event precedes the Microscopy and Microanalysis 2016 meeting held this coming July 25-28 in Columbus, OH.
This Pre-Meeting Congress reviews basic methodologies in the analysis of crystalline materials using electron diffraction (specific speakers and topics can be found listed above and in the attached flyer). Both SEM and TEM methods are featured. This Congress will feature eight invited speakers, each an internationally renowned expert in the utilization of electron diffraction methods for analyzing one or more of the following structural properties of crystalline materials: phase/symmetry, orientation, defects, and strain. Each speaker will describe the fundamental physics, explain the basic methodologies and approaches, and highlight key recent research findings and/or important technique developments. This session provides an excellent opportunity for electron microscopists to review SEM and TEM methodologies based on electron diffraction, and gain new insights on the latest advances and applications of state-of-the-art diffraction methods. This workshop is also extremely beneficial to students and researchers new to SEM/TEM.
Registration coincides with registration for the Microscopy and Microanalysis 2016 meeting at the link provided above. We look forward to your presence at this event and we greatly acknowledge and appreciate financial support from our sponsors.
ORGANIZERS: Microscopy Society of America (MSA) Focused Interest Group (FIG) on Electron Crystallography and Automated Mapping Techniques (ECAMT)
Jörg Wiezorek, University of Pittsburgh Yoosuf Picard, Carnegie Mellon University Sergei Rouvimov, University of Notre Dame Robert Stroud, nanoMegas
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Title-Subject: [Filtered] TEM- Looking for best particle counting software?
Message: Hi: I am looking for the best software option(s) for counting and sizing particles in a TEM micrograph. In my previous experiences, I found most software that I had experimented with to be cumbersome and unreliable at distinguishing particles, especially ones with low contrast. Any recommendations of software that users have found to be reliable and easy to use would be most welcome. SK
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Hello Dear List We have a problem with our Emitech K550X sputter coater (version2.1 -fully digitized): The argon inlet valve remains constantly open and vacuum cannot establish. We can still coat if we keep the argon regulator closed from the cylinder, so that no argon enters the chamber. Can the problem be something simple or there is an electronic fault behind? If anybody has any suggestions please advice. Best regards yorgos
I know this is a long shot, but does anyone out there have a manual for a Commonwealth Scientific IBS-250 or 600 Ion Beam Power supply? My usual sources have drawn a blank and Veeco has not gotten back to me.
-Jerry
==============================Original Headers============================== 2, 47 -- From jerry.biehler-at-gmail.com Fri May 27 14:27:29 2016 2, 47 -- Received: from mail-pa0-f45.google.com (mail-pa0-f45.google.com [209.85.220.45]) 2, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4RJRTu9017391 2, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2016 14:27:29 -0500 2, 47 -- Received: by mail-pa0-f45.google.com with SMTP id bz2so13042945pad.1 2, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2016 12:27:35 -0700 (PDT) 2, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 47 -- d=gmail.com; s=20120113; 2, 47 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 2, 47 -- :to; 2, 47 -- bh=nJff40262b5WzXS34QZ24fEeRarBVq2EfplzqDoutxY=; 2, 47 -- b=PpmPmG4JxDjrPgrs9JE3n6MRxMWOmZr2WHBPjCH+NwVhCwbeZFV1KzI4wgO4fjs8a1 2, 47 -- aHRsVn3E4nwkqw4WvjCdkob/25gO4x6fN72W80ndSVUx8S3cxyyNdGvg/3+kd7Oynouj 2, 47 -- ucENTTiPdWZ8IiV1w/AWRrVUwMpSNYmgKCKk3rMoHxywu82kcTMYWAdfaYUuw76AbqyS 2, 47 -- cZmVOZc1uZxqG8KvcmVFekUKI5mYG5EZC5YwMtSBDjxdhm/TGlPNnC3x4ifmxjzi5QYe 2, 47 -- /Zzj5II2iHDEHlGqcawEouwGZMPbd2vU7Ux4F0QXwuk3A3zHSVkyII5qXvFBl9K2gatm 2, 47 -- n/fg== 2, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 47 -- d=1e100.net; s=20130820; 2, 47 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 2, 47 -- :subject:message-id:date:to; 2, 47 -- bh=nJff40262b5WzXS34QZ24fEeRarBVq2EfplzqDoutxY=; 2, 47 -- b=Jv4xABTK49C2J3a7ZM+7M4mSUimekK/igpDiurp3DVKzsYqcG7/hOIh9ZVPFnRlXHs 2, 47 -- BXf2ffqNETl/crNt7274yqbL+8Qg7gQ09cKz8ZRlza57q2DGtvvWD+mKBFKWEcZGFvuP 2, 47 -- EcSR7IPr1P7sLzcXdiBhAQV2qh3o4hHAHr815eB3sOzLv11CvYm/Aeg7IUbvdIJkkRz9 2, 47 -- O4lcjPTbYdVOinpGmH/cbSHPHpcfe1W1q/v3srZOyydoRs1iPbLf4S5mzlFOWuYe0ymZ 2, 47 -- 7uZegUuRAuMpMNPw9oSF6u8zIJzugyz+qN7rrdxd82L6sP1+/FnzgEBKnJij1JSXFUwv 2, 47 -- gbOg== 2, 47 -- X-Gm-Message-State: ALyK8tKYjFnvh7IORwYYSLFyL4KrtgNNo57NOGY22EFjQEVKrfdkWeNlqK+NUU8Qh/0+XQ== 2, 47 -- X-Received: by 10.66.65.133 with SMTP id x5mr25154180pas.108.1464377254413; 2, 47 -- Fri, 27 May 2016 12:27:34 -0700 (PDT) 2, 47 -- Received: from [192.168.1.251] (c-73-25-87-83.hsd1.or.comcast.net. [73.25.87.83]) 2, 47 -- by smtp.gmail.com with ESMTPSA id m81sm2227520pfi.51.2016.05.27.12.27.33 2, 47 -- for {Microscopy-at-microscopy.com} 2, 47 -- (version=TLSv1/SSLv3 cipher=OTHER); 2, 47 -- Fri, 27 May 2016 12:27:33 -0700 (PDT) 2, 47 -- From: Jerry Biehler {jerry.biehler-at-gmail.com} 2, 47 -- Content-Type: text/plain; 2, 47 -- charset=us-ascii 2, 47 -- Mime-Version: 1.0 (1.0) 2, 47 -- Subject: Manual for Commonwealth IBS-250 2, 47 -- Message-Id: {1A9098A9-D375-4FDA-99E2-5267704F8F59-at-gmail.com} 2, 47 -- Date: Fri, 27 May 2016 12:27:33 -0700 2, 47 -- To: Microscopy-at-microscopy.com 2, 47 -- X-Mailer: iPhone Mail (13F69) 2, 47 -- Content-Transfer-Encoding: 8bit 2, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4RJRTu9017391 ==============================End of - Headers==============================
We managed to fix the problem by hammering (gently) the metal valve that was probably stuck Thanks to everybody who replied yorgos
-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: Friday, May 27, 2016 9:53 PM To: eikonika-at-otenet.gr
Hello Dear List We have a problem with our Emitech K550X sputter coater (version2.1 -fully digitized): The argon inlet valve remains constantly open and vacuum cannot establish. We can still coat if we keep the argon regulator closed from the cylinder, so that no argon enters the chamber. Can the problem be something simple or there is an electronic fault behind? If anybody has any suggestions please advice. Best regards yorgos
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Email: dsmrc.bulletin-at-gmail.com Name: Sithu Tun
Organization: myanmar medical research
Title-Subject: [Filtered] SEM STAGE IS NOT INITIALISED
Message: My evo ls 10 zeiss sem has a problem of stage is not initialised. please kindly answer me how to solve this. With respect
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Email: 13qw9-at-queensu.ca Name: Qiang
Organization: Queen's University
Title-Subject: [Filtered] FIB micropillar methods
Message: Dear all, Are there anybody can provide a good recipe for FIB micropillars? My circular pillar ends up with a ditch surrounding the bottom, which makes it difficult to measure the exact height of the pillar. Thank you!
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I would like to know if the plasma of XEI Evactron system is generated by induction or not.
Thanks
-- Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
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Email: rms-at-angstrom.us Name: Bob Sommerville
Organization: Angstrom Scientific Inc
Title-Subject: [Filtered] Technical Sales Position Available Message: Position: Technical Sales Representative Mid-Western US Position Description: This is an exciting and established territory with primary emphasis on selling and supporting the Hitachi TM series of table-top scanning electron microscopes. In addition, Angstrom Scientific Inc. represents a number of leading companies in the electron microscopy and related markets. The ideal candidate will preferably have a demonstrated track record of success ( 2-3 years) selling technical products into the academic, research and industrial markets. Optical microscopy or SEM/TEM related experience is a definite plus. Academic background should be at min an associates degree in science (BS or masters preferred) The successful candidate will live in OH MI or IL. Angstrom Scientific Inc is a growing distributor of products targeted to the nanotechnology, research and semiconductor markets with exciting new and novel technology Angstrom Scientific Inc offers competitive salary and benefits, commission, car allowance and expenses. We are an equal opportunity employer. Interested candidates should submit their resume together with salary history to: Bob Sommerville, General Sales Manager, Angstrom Scientific Inc. e-mail: rms-at-angstrom.us
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Email: cni-at-udel.edu Name: Chaoying Ni
Organization: University of Delaware
Title-Subject: [Filtered] Research associate position at microscopy center
Message: Research Associate - Materials Science and Engineering (W.M. Keck Center for Advanced Microscopy and Microanalysis, http://www.camm.udel.edu)
Pay Grade: 28E
Deadline: June 18, 2016
Under limited supervision, performs a variety of complex technical laboratory duties including the design and construction of test equipment. Supervises and trains personnel. The principal emphasis is on working independently and exercising personal initiative in conducting experiments using complex electron and light microscopes, scanning probe microscopes and associated sample preparation equipment and experiment devices.
MAJOR RESPONSIBILITIES:
* Performs a variety of technical duties involved in conducting electron microscopic characterization and analysis to obtain data in support of research activities. * Performs contract work or experimental consultation to support industrial collaborations. * Sets up instrumentation; calibrates equipment and repairs equipment problems. * Procures and maintains material stock. * Helps to set and maintain proper laboratory safety standards. * Develops and modifies experimental procedures. * Trains (written and oral) graduate students and post-doctors for using electron microscopes and related equipment. * Prepares tables, graphs and report. * Performs other related duties as assigned.
QUALIFICATIONS:
Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a relevant field as well as experience with the necessary physical, electronic and chemical systems. Supervisory experience preferred. Related progressive experience beyond a high school diploma or GED may be substituted for required education or additional related education may be substituted for required experience. Requires knowledge of laboratory techniques, procedures and instrumentation;
Requires abilities to read and interpret complex operations manuals, drawings and design, interpret problems and design equipment for the particular tasks, assume responsibility and take initiative to perform tasks, work independently, train and instruct others, and communicate effectively and interact well with people of all ages and diverse backgrounds.
OCCUPATIONAL EXPOSURES:
May be required to use personal protective equipment to prevent exposure to hazardous materials.
When applying please submit a one-page cover letter and your resume as one document. Also, please remember to provide names, addresses and telephone number of at least three references in the online application.
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Employment offers will be conditioned upon successful completion of a criminal background check. A conviction will not necessarily exclude you from employment.
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Postdoctoral Research Associate in Environmental Mineralogy
The Soil and Environmental Biogeochemistry Group in the Department of Ecosystem Science and Management at the University of Wyoming, Laramie, WY, USA is seeking candidates for a postdoctoral position in Environmental Mineralogy to work on mineral formation using in situ liquid cell transmission electron microscopy (LC-TEM). The 3-year project is funded by the U.S. Department of Energy (FY2016 – FY2019), which will start on August 1, 2016. The starting annual salary is $40,000 with full benefits with possible raises each year.
Manganese i(Mn) oxides are versatile minerals and have both economic and environmental significance, but little is known regarding their formation mechanisms. This project will determine nucleation and particle growth mechanisms of layered and tunneled manganese (Mn) oxides using cutting-edge in situ liquid cell transmission electron microscopy (LC‑TEM). Synchrotron X-ray techniques, such as X-ray atomic pair distribution function (PDF) analysis, will also be used.
Requirement for consideration of applications includes: 1) A Ph.D. degree in appropriate areas of Mineralogy, Materials Science, Geochemistry, Chemistry, or a closely related field. 2) Proficient in operating high-resolution TEM (HR-TEM) instruments and have extensive experience in HR-TEM characterization of inorganic materials. 3) LC-TEM experience is not required, but preferred. 4) Work independently. 5) Excellent written and verbal communication skills in English 6) Candidates must be legally able to work in the United States
This is a collaborative project with a staff scientist with expertise on LC-TEM analysis at the Pacific Northwest National Laboratory, Richland, WA. The major portion of the research will be conducted at the University of Wyoming. The post‑doc will be trained at PNNL for LC-TEM analysis. The post-doc will have opportunities to attend academic conferences, teach or give guest lectures for a Mineralogy course, and mentor graduate students in the group.
Applicants should submit a cover letter, curriculum vita, and names and contact information for at least 3 referees who can provide a letter of recommendation upon request, to Dr. Mengqiang (Mike) Zhu at mzhu6-at-uwyo.edu. In the cover letter, applicants need to state research interests and experience addressing the criteria listed above and give a date of availability. All documents must be submitted as a single pdf file. The application review will start immediately and continues until the position is filled. Anticipated project start date is August 1, 2016, but later start dates for the successful candidate can be considered. For more information regarding the position, please contact Dr. Mengqiang Zhu (307-766-5523, mzhu6-at-uwyo.edu).
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I have inherited an old Sorvall glass knife maker that we would like to use (modify, i fnecessary) for scoring and breaking 2mm thick glass into one inch squares. No one remembers how to use it... Do any of you have a manual for the thing?
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 3, 20 -- From tina-at-pbrc.hawaii.edu Wed Jun 1 22:19:40 2016 3, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u523JddU018879 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jun 2016 22:19:40 -0500 3, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 3, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u523K3U9005196 3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jun 2016 17:20:03 -1000 (HST) 3, 20 -- Received: from localhost (tina-at-localhost) 3, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u523K21m005192 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jun 2016 17:20:03 -1000 (HST) 3, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 3, 20 -- Date: Wed, 1 Jun 2016 17:20:02 -1000 (HST) 3, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 3, 20 -- X-X-Sender: tina-at-b1000 3, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 20 -- Subject: Instruction manual for Sorvall Glass Knife Maker 3, 20 -- Message-ID: {Pine.GSO.4.64.1606011656250.551-at-b1000} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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I have already suggested ImageJ, but perhaps the list knows of other good programs for doing this.
Name: Piragash Kumar R M School: Manipal Institute of Technology Grade/Education Level: Graduate Location: Manipal, IN Email: prakashkumar03-at-gmail.com
Dear Tina,
hopefully you got a lot of answers (??) .
Would it be possible to tell which one of all those found by Google (cf. https://www.google.com/search?q=sorvall+glass+knife+maker) you really mean? (since - if I remember correctly - also the former LKB - or later on - the Leica Knife Maker sometimes were called { Sorvall....} )...
For the LEICA EM KMR2, the REICHERT,LKB-2nd Generation as well as for the LKB knife breaker_7800B_, 7800, 7801, 2178 I do have manuals in my files....
But if ist really the "SORVALL Glass Knife Maker" as found at: http://microtomeserviceco.com/index.php?route=product/product&product_id=57 you might get info or perhaps also a copy of the original Manual via:
4072 Rusty Pine Lane Liverpool NY 13090 U.S.A. 315-451-1404 Mitch-at-MicrotomeServiceCo.com (Disclaimer: no affiliation with, nor any financial interest)
Unfortunately I can't help with an original manual but perhaps -at- http://facpub.stjohns.edu/~trombetl/lab%20manual.htm you can find sufficing information at least for a try....*) Best wishes, good luck , cordially yours, Wolfgang
Wolfgang MUSS Retired (still member of MSA?) SALZBURG, AUSTRIA
* PS: SORVALL GLASS KNIFE MAKER - INSTRUCTIONS
I - Wash glass rectangles with alconox and scrub brush.
II - Reducing glass rectangles to 2.54cm (1 in) squares.
1 - Remove plastic cover.
2 - Center glass using a ruler on the top plate against the back stop plate. Support ends of glass rectangle if the rectangle is very long otherwise excessive pressure will be placed on the score line.
3 - Check glass to ensure that the manufacturer's score is down (This side is identified by minute ridges along the edge)
4 - Turn the handwheel clockwise while holding the glass in place. After the bumpers have made contact with the glass, complete on more full turn of the handwheel.
The glass should be held securely. Try to rock the anvil to see if the glass is secure. If the anvil can be moved, continue turning the hand wheel RAPIDLY until the anvil is tight.
5 - Pull the plunger adjustment shaft out to prepare for the short stroke.
6 - Pull the scoring shaft all the way out. Depress the button and push the scoring shaft back with one even stroke to score the glass.
7 - SLOWLY, GENTLY, and CAREFULLY turn the breaking control knob clockwise until the glass breaks.
8 - Turn the handwheel counterclockwise to raise the clamp and anvil NEVER TURN THE BREAK CONTROL KNOB COUNTERCLOCKWISE IT WILL RESET AUTOMATICALLY NEVER RAISE THE CLAMP ARM TO ITS HIGHEST POSITION
9 - Remove glass by placing your fingers on top of the glass and sliding the pieces toward you.
Repeat all steps until glass is broken into 2.54 cm squares.
_______________________________________________________ III - Preparation of Knives
1 - Inspect the corners and sides of the square carefully. Select the corner that is the smoothest ( with the fewest score marks) and the most nearly perpendicular to the 2,54cm square.
2 - Place the glass square on the top plate with the selected corner seated in the back stop plate registration notch. The scored edges of the glass square should now be up
3 - Push the positioner slide lightly against the corner of the glass facing the operator.
4 - Hold the positioner slide lightly in place while turning the handwheel clockwise to clamp the glass to the top plate.
5 - Push the plunger adjustment shaft IN for a long stroke.
6 - Pull the positioner back so it is flush against the top plate surface.
7 - Pull the scoring shaft all the way out. Depress the button and push the scoring shaft back in one even stroke.
8 - Place plastic cover over the instrument.
9 - SLOWLY AND CAREFULLY turn the breaking control knob CLOCKWISE until the glass breaks.
10 - Remove the plastic cover.
11 - Turn the handwheel counterclockwise to raise the clamp arm and anvil. NEVER TURN THE BREAK CONTROL KNOB COUNTERCLOCKWISEIT WILL RESET AUTOMATICALLY NEVER RAISE THE CLAMP ARM TO ITS HIGHEST POSITION
Inspect each glass knife
The outer one-third of the knife edge that parallels the stress line will be the sharpest section.
The final test of the acceptability of the glass knife is to examine the knife edge for "whiskers". This is done under a binocular stereo microscope with bright field illumination from above against a dark background.
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I have inherited an old Sorvall glass knife maker that we would like to use (modify, i fnecessary) for scoring and breaking 2mm thick glass into one inch squares. No one remembers how to use it... Do any of you have a manual for the thing?
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 3, 20 -- From tina-at-pbrc.hawaii.edu Wed Jun 1 22:19:40 2016 3, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u523JddU018879 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jun 2016 22:19:40 -0500 3, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 3, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u523K3U9005196 3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jun 2016 17:20:03 -1000 (HST) 3, 20 -- Received: from localhost (tina-at-localhost) 3, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u523K21m005192 3, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 1 Jun 2016 17:20:03 -1000 (HST) 3, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 3, 20 -- Date: Wed, 1 Jun 2016 17:20:02 -1000 (HST) 3, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 3, 20 -- X-X-Sender: tina-at-b1000 3, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 20 -- Subject: Instruction manual for Sorvall Glass Knife Maker 3, 20 -- Message-ID: {Pine.GSO.4.64.1606011656250.551-at-b1000} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 46, 21 -- From wij.muss-at-aon.at Thu Jun 2 10:21:54 2016 46, 21 -- Received: from bsmtp3.bon.at (bsmtp3.bon.at [213.33.87.17]) 46, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52FLswv010473 46, 21 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 10:21:54 -0500 46, 21 -- Received: from MussTHINK (unknown [93.83.25.98]) 46, 21 -- by bsmtp3.bon.at (Postfix) with ESMTPSA id 3rL9vH2SYdz5tlJ; 46, 21 -- Thu, 2 Jun 2016 17:22:19 +0200 (CEST) 46, 21 -- From: "MUSS Wolfgang SCUR Secretary" {wij.muss-at-aon.at} 46, 21 -- To: {tina-at-pbrc.hawaii.edu} 46, 21 -- Cc: {microscopy-at-microscopy.com} 46, 21 -- Subject: [Microscopy] Re: Instruction manual for Sorvall Glass Knife Maker 46, 21 -- Date: Thu, 2 Jun 2016 17:22:20 +0200 46, 21 -- Message-ID: {005c01d1bce2$8f1601f0$ad4205d0$-at-aon.at} 46, 21 -- MIME-Version: 1.0 46, 21 -- Content-Type: text/plain; 46, 21 -- charset="iso-8859-1" 46, 21 -- X-Mailer: Microsoft Outlook 15.0 46, 21 -- Thread-Index: AdG84I8lvioDocnWTFS6UsPlREDazA== 46, 21 -- Content-Language: de-at 46, 21 -- Content-Transfer-Encoding: 8bit 46, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u52FLswv010473 ==============================End of - Headers==============================
This is actually an interesting question, as I am curious what people generally use for image analysis. It seems like there is a bewildering array of free software, manufacturer supplied software, and third-party software out there. The methods used by a lot of these software packages seem pretty black-box.
In terms of free software, I second the suggestion of ImageJ; it works well with regular optical micrographs. Another option is to look to the maker of the microscope you are using, I think that most of the makers provide at least rudimentary particle analysis software with the equipment. Nikon provides a package called 'NIS-Elements' (http://www.nikon.com/products/microscope-solutions/lineup/img_soft/), though it is not free.
Apart from that, there are other programs that are optimized for EM or AFM that might work, such as gwyddion (free), SPIP (not free), or with an EM focus, Digital Micrograph (from Gatan, for EM but takes any greyscale image and I believe there is a free-as-in-beer demo version available)
Other than those options, a quick google search turned up several third party commercial image analysis packages that claim to be able to do particle analysis. I have not looked at any of these in depth, so I cannot make any recommendations for these.
-Jake Jokisaari
On 06/02/2016 08:15 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } ************************************************************************************* } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } ************************************************************************************* } } I have already suggested ImageJ, but perhaps the list knows of other good programs for doing this. } } Name: } Piragash Kumar R M } School: } Manipal Institute of Technology } Grade/Education Level: } Graduate } Location: } Manipal, } IN } Email: } prakashkumar03-at-gmail.com } Subject: } Counting dots in an image; microscopy } Your Question: } I would like to know about the available methods and tools to count the number of dots in an optical microscope image and thus find the percentage area occupied by the dots in the image. I work with Nikon Eclipse optical microscope and the images are captured at 1000x magnification using Clemex Captiva image acquisition platform (Clemex CCD camera). Please help me and thanks in advance } } } ==============================Original Headers============================== } 4, 64 -- From oshel1pe-at-cmich.edu Thu Jun 2 07:54:24 2016 } 4, 64 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) } 4, 64 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52CsOCS018797 } 4, 64 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 07:54:24 -0500 } 4, 64 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged)) } 4, 64 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u52Csnc9031278 } 4, 64 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:54:50 -0400 } 4, 64 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by } 4, 64 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server } 4, 64 -- (TLS) id 14.3.248.2; Thu, 2 Jun 2016 08:54:50 -0400 } 4, 64 -- Received: from na01-by2-obe.outbound.protection.outlook.com (141.209.2.100) by } 4, 64 -- mail.cmich.edu (141.209.15.90) with Microsoft SMTP Server (TLS) id } 4, 64 -- 14.3.248.2; 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Thanks for all the replies which, as usual, asked even more questions. Wai Pang Chan sent a link to the full manual for the Sorvall/DuPont glass knife maker:
On the third page down there's a picture of the thing, for those who asked.
Now to see if we can make it break 2mm glass, because I've never been able to mod the LKB to do it.
Aloha, Tina
} I have inherited an old Sorvall glass knife maker that we would like to use } (modify, i fnecessary) for scoring and breaking 2mm thick glass into one } inch squares. No one remembers how to use it... Do any of you have a manual } for the thing? } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 22 -- From tina-at-pbrc.hawaii.edu Thu Jun 2 13:17:17 2016 8, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52IHHSd023891 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:17:17 -0500 8, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u52IHhID012105 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- Received: from localhost (tina-at-localhost) 8, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u52IHhxZ012101 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 8, 22 -- Date: Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 22 -- X-X-Sender: tina-at-b1000 8, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 22 -- Subject: Found - Re: Instruction manual for Sorvall Glass Knife Maker 8, 22 -- In-Reply-To: {Pine.GSO.4.64.1606011656250.551-at-b1000} 8, 22 -- Message-ID: {Pine.GSO.4.64.1606020812440.11926-at-b1000} 8, 22 -- References: {Pine.GSO.4.64.1606011656250.551-at-b1000} 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
We have one of these, but the sueded leather disc inside the handwheel assembly slips such that the glass is not held tightly enough against the anvil. Does anyone have a do-it-yourself solution?
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
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Hi, Everyone-
Thanks for all the replies which, as usual, asked even more questions. Wai Pang Chan sent a link to the full manual for the Sorvall/DuPont glass knife maker:
On the third page down there's a picture of the thing, for those who asked.
Now to see if we can make it break 2mm glass, because I've never been able to mod the LKB to do it.
Aloha, Tina
} I have inherited an old Sorvall glass knife maker that we would like } to use (modify, i fnecessary) for scoring and breaking 2mm thick glass } into one inch squares. No one remembers how to use it... Do any of you } have a manual for the thing? } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } ********************************************************************** } ****** }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 22 -- From tina-at-pbrc.hawaii.edu Thu Jun 2 13:17:17 2016 8, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52IHHSd023891 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:17:17 -0500 8, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u52IHhID012105 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- Received: from localhost (tina-at-localhost) 8, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u52IHhxZ012101 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 8, 22 -- Date: Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 22 -- X-X-Sender: tina-at-b1000 8, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 22 -- Subject: Found - Re: Instruction manual for Sorvall Glass Knife Maker 8, 22 -- In-Reply-To: {Pine.GSO.4.64.1606011656250.551-at-b1000} 8, 22 -- Message-ID: {Pine.GSO.4.64.1606020812440.11926-at-b1000} 8, 22 -- References: {Pine.GSO.4.64.1606011656250.551-at-b1000} 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 35 -- From LettJ-at-ent.wustl.edu Thu Jun 2 13:52:22 2016 18, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 18, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52IqMku016492 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:52:22 -0500 18, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 18, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u52IZJVP006105 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:52:49 -0500 18, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 18, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 239k9y2qpx-1 18, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 02 Jun 2016 13:52:49 -0500 18, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 18, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 18, 35 -- Thu, 2 Jun 2016 13:52:50 -0500 18, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 18, 35 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 18, 35 -- Subject: RE: Sorvall Glass Knife Maker 18, 35 -- Thread-Topic: Sorvall Glass Knife Maker 18, 35 -- Thread-Index: AdG8/+a4vHDDP7ODTSOqodfdI47G4Q== 18, 35 -- Date: Thu, 2 Jun 2016 18:52:48 +0000 18, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F42ECB-at-pcfmbx01.wusm-pcf.wustl.edu} 18, 35 -- Accept-Language: en-US 18, 35 -- Content-Language: en-US 18, 35 -- X-MS-Has-Attach: 18, 35 -- X-MS-TNEF-Correlator: 18, 35 -- x-originating-ip: [10.39.135.134] 18, 35 -- Content-Type: text/plain; charset="us-ascii" 18, 35 -- MIME-Version: 1.0 18, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.3,0.0.0000 18, 35 -- definitions=2016-06-02_05:2016-06-02,2016-06-02,1970-01-01 signatures=0 18, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 18, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 18, 35 -- engine=7.0.1-1603290000 definitions=main-1606020220 18, 35 -- Content-Transfer-Encoding: 8bit 18, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u52IqMku016492 ==============================End of - Headers==============================
No financial interest blah blah blah - but the Missouri shop seemed appropriate for Jaci.
Tamara
................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM
________________________________________ X-from: LettJ-at-ent.wustl.edu {LettJ-at-ent.wustl.edu} Sent: Thursday, June 02, 2016 12:54 PM To: Tamara Howard
We have one of these, but the sueded leather disc inside the handwheel assembly slips such that the glass is not held tightly enough against the anvil. Does anyone have a do-it-yourself solution?
Thank you,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lettj-at-ent.wustl.edu Website: http://otocore.wustl.edu
The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
Hi, Everyone-
Thanks for all the replies which, as usual, asked even more questions. Wai Pang Chan sent a link to the full manual for the Sorvall/DuPont glass knife maker:
On the third page down there's a picture of the thing, for those who asked.
Now to see if we can make it break 2mm glass, because I've never been able to mod the LKB to do it.
Aloha, Tina
} I have inherited an old Sorvall glass knife maker that we would like } to use (modify, i fnecessary) for scoring and breaking 2mm thick glass } into one inch squares. No one remembers how to use it... Do any of you } have a manual for the thing? } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } ********************************************************************** } ****** }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 22 -- From tina-at-pbrc.hawaii.edu Thu Jun 2 13:17:17 2016 8, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52IHHSd023891 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:17:17 -0500 8, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 8, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u52IHhID012105 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- Received: from localhost (tina-at-localhost) 8, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u52IHhxZ012101 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 8, 22 -- Date: Thu, 2 Jun 2016 08:17:43 -1000 (HST) 8, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 8, 22 -- X-X-Sender: tina-at-b1000 8, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 8, 22 -- Subject: Found - Re: Instruction manual for Sorvall Glass Knife Maker 8, 22 -- In-Reply-To: {Pine.GSO.4.64.1606011656250.551-at-b1000} 8, 22 -- Message-ID: {Pine.GSO.4.64.1606020812440.11926-at-b1000} 8, 22 -- References: {Pine.GSO.4.64.1606011656250.551-at-b1000} 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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==============================Original Headers============================== 18, 35 -- From LettJ-at-ent.wustl.edu Thu Jun 2 13:52:22 2016 18, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu (pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171]) 18, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u52IqMku016492 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:52:22 -0500 18, 35 -- Received: from pps.filterd (PCFPRFAGENT02.wusm-pcf.wustl.edu [127.0.0.1]) 18, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu (8.15.0.59/8.15.0.59) with SMTP id u52IZJVP006105 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Jun 2016 13:52:49 -0500 18, 35 -- Received: from mail.wusm-pcf.wustl.edu (pcfhub02.wusm-pcf.wustl.edu [10.39.163.73]) 18, 35 -- by PCFPRFAGENT02.wusm-pcf.wustl.edu with ESMTP id 239k9y2qpx-1 18, 35 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 18, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 02 Jun 2016 13:52:49 -0500 18, 35 -- Received: from PCFMBX01.wusm-pcf.wustl.edu ([10.39.163.61]) by 18, 35 -- PCFHUB02.wusm-pcf.wustl.edu ([10.39.163.73]) with mapi id 14.03.0181.006; 18, 35 -- Thu, 2 Jun 2016 13:52:50 -0500 18, 35 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 18, 35 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 18, 35 -- Subject: RE: Sorvall Glass Knife Maker 18, 35 -- Thread-Topic: Sorvall Glass Knife Maker 18, 35 -- Thread-Index: AdG8/+a4vHDDP7ODTSOqodfdI47G4Q== 18, 35 -- Date: Thu, 2 Jun 2016 18:52:48 +0000 18, 35 -- Message-ID: {60BCCE5EB396FA46B60FF9BEA2E1353225F42ECB-at-pcfmbx01.wusm-pcf.wustl.edu} 18, 35 -- Accept-Language: en-US 18, 35 -- Content-Language: en-US 18, 35 -- X-MS-Has-Attach: 18, 35 -- X-MS-TNEF-Correlator: 18, 35 -- x-originating-ip: [10.39.135.134] 18, 35 -- Content-Type: text/plain; charset="us-ascii" 18, 35 -- MIME-Version: 1.0 18, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.96,1.0.3,0.0.0000 18, 35 -- definitions=2016-06-02_05:2016-06-02,2016-06-02,1970-01-01 signatures=0 18, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 phishscore=0 18, 35 -- adultscore=0 bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 18, 35 -- engine=7.0.1-1603290000 definitions=main-1606020220 18, 35 -- Content-Transfer-Encoding: 8bit 18, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u52IqMku016492 ==============================End of - Headers==============================
Another place to look, though it’s aimed more at plant biologists, is http://www.plant-image-analysis.org/, although it’s assumed that most users are familiar with ImageJ, to some extent. The point-detection plugins are pretty easy in ImageJ.
Is there a similar directory of image analysis packages for other applications? My feeling would be, probably not, as it’d be enormous…
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU
T 61 2 6246 5475 E rosemary.white-at-csiro.au
On 3/06/2016 3:17 am, "joki-at-umich.edu" {joki-at-umich.edu} wrote:
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we recently obtained a second-hand Active Vibration Isolation system made by Integrated Dynamics Engineering (IDE). The system comes with a "MAXCON-1000" controller which is connected to the plate with 4 motor and 4 isolator cables. We think that the system also has transport protection clamps that need to be removed before use, but unfortunately we don't have a manual for the system.
If you have a manual, software or any other information for this system it would be great if you could send it to me.
Thanks you, Stefan
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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)
Message: Dear Colleagues,
I am a relatively new user of TEM, getting started with STEM now. As in STEM mode, the resolution depends on probe size, so I am really curious to calculate the probe size for my STEM images. I found a formula to calculate probe diameter using CBED discs in William's book but it requires bragg angle (thetaB) in calculations. Is there any simple way to calculate the probe size?
Thanks
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We have recently had a world leading microscopy client open up several retained opportunities throughout the US. The ideal candidate would have an expertise in microscopy/imaging, and a comfort working directly with customers to promote the sales of the companies microscopy product line (material science, forensic, and image analysis). Please contact me directly to learn more at - sk-at-personifysearch.com or (800) 875-6188, ext.153. Thanks! Sarah
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Email: dblackburn2000-at-yahoo.com Name: D Blackburn
Message: We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because they are so resistant to chemicals. However, we've recently realized that the bottles grow very brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others experienced this? Is it a common, well-known problem?
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My University is trying to decide whether it is appropriate to charge overhead on users of our facilities from both government agencies and other universities. I would like to know what the policy is for other universities, thanks, Ken
==============================Original Headers============================== 2, 34 -- From prvs=961dfd989=klivi-at-jhu.edu Thu Jun 9 11:10:52 2016 2, 34 -- Received: from IronMTW2.johnshopkins.edu (ironmtw2.johnshopkins.edu [128.220.39.120]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u59GAp1o013700 2, 34 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2016 11:10:52 -0500 2, 34 -- X-IronPort-AV: E=Sophos;i="5.26,445,1459828800"; 2, 34 -- d="scan'208";a="181965678" 2, 34 -- Received: from esgebex5.win.ad.jhu.edu ([10.15.89.10]) 2, 34 -- by IronMTW2.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 09 Jun 2016 12:11:44 -0400 2, 34 -- Received: from ESGEBEX1.win.ad.jhu.edu (10.15.89.6) by ESGEBEX5.win.ad.jhu.edu 2, 34 -- (10.15.89.10) with Microsoft SMTP Server (TLS) id 15.0.1156.6; Thu, 9 Jun 2, 34 -- 2016 12:11:43 -0400 2, 34 -- Received: from ESGEBEX1.win.ad.jhu.edu ([fe80::9468:4888:5772:702d]) by 2, 34 -- ESGEBEX1.win.ad.jhu.edu ([fe80::9468:4888:5772:702d%31]) with mapi id 2, 34 -- 15.00.1156.000; Thu, 9 Jun 2016 12:11:43 -0400 2, 34 -- From: KEN Livi {klivi-at-jhu.edu} 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 34 -- CC: KEN Livi {klivi-at-jhu.edu} 2, 34 -- Subject: EM rates and overhead 2, 34 -- Thread-Topic: EM rates and overhead 2, 34 -- Thread-Index: AQHRwmmcZciUiRTnPUSwUqx7iAANxw== 2, 34 -- Date: Thu, 9 Jun 2016 16:11:42 +0000 2, 34 -- Message-ID: {3A42920C-1EDE-44D5-9E67-29F1CC563C29-at-jhu.edu} 2, 34 -- Accept-Language: en-US 2, 34 -- Content-Language: en-US 2, 34 -- X-MS-Has-Attach: 2, 34 -- X-MS-TNEF-Correlator: 2, 34 -- x-ms-exchange-messagesentrepresentingtype: 1 2, 34 -- x-ms-exchange-transport-fromentityheader: Hosted 2, 34 -- x-originating-ip: [10.181.198.101] 2, 34 -- Content-Type: text/plain; charset="us-ascii" 2, 34 -- Content-ID: {910A49FB5627CF4485448F257CE719D3-at-exchange.johnshopkins.edu} 2, 34 -- MIME-Version: 1.0 2, 34 -- Content-Transfer-Encoding: 8bit 2, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u59GAp1o013700 ==============================End of - Headers==============================
I think, yes, it is entirely appropriate to charge external entities overhead to for use of your instruments. I'm surprised your university is just now trying to decided if this is proper policy. In many places it is required (such as at Central Michigan, where I am). The overhead is just recovering operating costs that the university covers for its in-house people. So it charges them to the out-house people.
Phil ________________________________________ X-from: klivi-at-jhu.edu {klivi-at-jhu.edu} Sent: Thursday, June 09, 2016 12:33 To: Oshel, Philip Eugene
Dear University Lab Managers and such,
My University is trying to decide whether it is appropriate to charge overhead on users of our facilities from both government agencies and other universities. I would like to know what the policy is for other universities, thanks, Ken
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I would like to inform you that the EELS and XAS database website has been completely rewritten and is now accessible at https://eelsdb.eu/ and can be used without registration.
We have performed many changes, in particular an improved design, a streamlined submission process and an online plotting function. An application-programming interface (API) has also been created, allowing external tools and software (such as hyperspy) to easily access the information held within the database.
I hope you will like this new version of the website and I encourage everyone to submit their spectra to the website!
Best,
Luc --
Postdoctoral researcher STEM-EELS specialist
Advanced Microscopy Laboratory (LMA) Instituto de Nanocienca de Aragon (INA) Zaragoza, España --- The EELS database --- https://eelsdb.eu
==============================Original Headers============================== 11, 23 -- From Luc.Lajaunie-at-cnrs-imn.fr Fri Jun 10 05:10:40 2016 11, 23 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5AAAdGn019360 11, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jun 2016 05:10:39 -0500 11, 23 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 11, 23 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id u5AABXPB005827 11, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jun 2016 12:11:33 +0200 11, 23 -- Received: from d-agenda.cnrs-imn.fr (d-agenda.cnrs-imn.fr [193.52.97.16]) 11, 23 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id u5AABWMu021648 11, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jun 2016 12:11:32 +0200 11, 23 -- from: "Luc Lajaunie" {Luc.Lajaunie-at-cnrs-imn.fr} 11, 23 -- X-Forward: 155.210.191.34 11, 23 -- content-type: text/plain; charset="utf-8" 11, 23 -- date: Fri, 10 Jun 2016 12:11:32 +0200 11, 23 -- subject: New version of the EELS and XAS Database 11, 23 -- MIME-Version: 1.0 11, 23 -- to: Microscopy-at-microscopy.com 11, 23 -- message-id: {c1d-575a9280-d-389c6240-at-145562469} 11, 23 -- User-Agent: SOGoMail 2.3.7a 11, 23 -- X-Miltered: at b-mail2 with ID 575A9255.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 11, 23 -- X-j-chkmail-Enveloppe: 575A9255.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Luc.Lajaunie-at-cnrs-imn.fr} 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u5AAAdGn019360 ==============================End of - Headers==============================
D Blackburn, You'll receive more accurate information from a Nalgene specialist, but yes, plastic bottles do age out as the plasticizers come out. If any of your bottles start to discolor or craze, I'd start to distrust them. I believe light accelerates the degradation, and the internet reports "denatured alchohol" as another possible accelerator. I'd also wonder if the platicizers are affecting your samples.
They don't 'explode' as much as they simply 'shatter'. It is startling, though.
Good luck, Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan, MCDB Dept.
On Wed, Jun 8, 2016 at 7:55 PM, {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: dblackburn2000-at-yahoo.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } dblackburn2000-at-yahoo.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: dblackburn2000-at-yahoo.com Name: D Blackburn } } Organization: Trinity College } } Title-Subject: [Filtered] exploding Nalgene bottles? } } Message: We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because } they are so resistant to chemicals. However, we've recently realized that the bottles grow very } brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a } result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others } experienced this? Is it a common, well-known problem? } } } Login Host: 24.250.10.154 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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Dear All, Here is a compilation of the responses to my question (minus the out-of-office responses)…
Original message:
Dear University Lab Managers and such,
My University is trying to decide whether it is appropriate to charge overhead on users of our facilities from both government agencies and other universities. I would like to know what the policy is for other universities, thanks, Ken ____________________________________________________ Responses (identification of places and names have been edited out)...
Here at *** we only charge overhead on industrial users, and Government users (if they should arise). Most of the time we extend the lower “in-house” rate to users from other Universities. We hope that this good will be reflected back on our users when they seek out use of other local university facilities that we may not have on campus. We do how ever not extend them a yearly maximum cap of usage fees as we would do for our internal users. If a user of our lab uses a certain number of hours per semester their billing is clipped once they’ve reached the cap. I believe this number is around 200 hours. This is easy since our microscopes are integrated into the clean room, and they may also use other systems in the lab. I hope this helps, and If you’d like more exact numbers let me know and I will search them out for you. ____________________________________________________ Our university facility has a fee structure for different user. Departmental users pay one rate, other departments and faculties another rate, and off campus users pay another higher rate. I suspect this type fee structure is similar to many other institutional facilities around the globe.
In the last round of university funding cuts (for the last 3 years) the University administration incorporated a surcharge to the fee for "Off Campus Users" of our facility. As a result, off campus users pay significantly more to use the facility and sadly the facility doesn't profit from the surcharge. ____________________________________________________ Yes to both. Every core on this campus is required to add overheads to anyone outside our institution unless there is a prior arrangement - e.g. our state *** partners.
You may want to get with the *** core. I ran the cell center there in the late 00s, and we had a schedule of charges based on where folks came from. Most were internal customers, but a couple were external, and we had to deal with the *** at ***. Another avenue is to follow a crada-like model. This allows you to set the charges for the user specifically based on a project. ____________________________________________________ *** charges overhead on all non-commercial users from outside *** except where there is a collaborative agreement to charge internal rates at each others campuses for investigators from the other campus. ____________________________________________________ Our administration is in discussion with our immediate neighbors ( *** and *** University) about this. The 3 schools share a graduate program and so arrangements about inter-campus Cores use has always been murky. That aside, we do charge overhead to other academic users an we double our rates to corporate users. I’ve not had to deal with government labs, so I can’t address that. ____________________________________________________ I think, yes, it is entirely appropriate to charge external entities overhead to for use of your instruments. I'm surprised your university is just now trying to decided if this is proper policy. In many places it is required (such as at ***, where I am). The overhead is just recovering operating costs that the university covers for its in-house people. So it charges them to the out-house people. ____________________________________________________ I work in a Core and The Boss actually hired someone to come and calculate overhead. We do include overhead in work with companies or non-educational facilities; we do not charge overhead to other universities or our internal users, although the pricing structure is different. We charge a base rate for *** Internal users, a 20% discounted rate for *** Center Members, 2x rate for outside universities and (approximately) 4x rate for corporate users. Companies are expecting overhead to be built-in. Most in academics ask “What’s overhead?” ? ____________________________________________________ We have no charges for outside academic users. We do charge non-academic users. ____________________________________________________ Just in case you (or the folks calculating your rates) were not aware of this resource:
Good luck. Accountants and Scientists certainly speak very different languages... ____________________________________________________ One governing rule that I thought was to guide the decision regarding this is that everyone is supposed to be charged the same rate. That means, at least for commercial entities, overhead has to be added to the hourly rate because research dollars have overhead charged on them when the grant comes into the University. Thus, it has to be added to those who don’t pay overhead up front.
However, other Universities have charged overhead on their research monies already. Following that logic, adding overhead to outside Universities would amount to charging double overhead on the research $ that a granting agency has given to any University. In effect, the granting agency and researchers would not be charged the same as others. I think this has the effect of discouraging cooperative research or forces researchers to find other ways to make rates more affordable. It also winds up hurting the core facilities since they don't often directly get the benefit of the overhead $ and use of the machines could be lessened.
However, from the viewpoint of a University’s administration, they would say that they are doing extra work without compensation. I can see validity of both sides. ____________________________________________________ We do charge hourly rates for both internal university use and outside university work, however you should look into your local regulations for these and other possible conditions: 1 We are required by state law to charge rates for outside university entities at a rate similar to a commercial analysis lab so that we are not unfairly competing. 2 Instruments acquired outside the US and imported 'duty-free' may not be used for commercial gain for a period of 5 years. 3 Instruments funded by federal grants may also have restrictions or limitations on who may be charged and what the charge may be. ____________________________________________________ At the university where I am at:
Inhouse users are charged for maintaining the instrument based on percent of usage and each user is responsible for there own preparation and supplies of materials.
Outsiders are charged extra for preparing samples (as some prep work is quick and easy while others are long and tedious) and for the time spent looking at there samples.
We conducted a study of cost and usage for In house and outside house EM work and found that universities who charge inhouse fees typically show a decrease in the amount of time the instrument is used while outside house is typically unaffected as a charge is being applied for the use of the instrument.
Many professors simply don't have the support or means to justify an EM study if there is a cheaper way to answer address the problem as EM work is often used as supporting evidence for the study that was already conducted. ____________________________________________________ We recharge off-campus users atleast 15% over the on-campus rate, and up to the market rate. If the funding source is not on-campus, then it is considered off-campus. Hence, having collaborators on-campus does not impact upon the rate.
-Ken
==============================Original Headers============================== 17, 36 -- From prvs=962daa4a8=klivi-at-jhu.edu Fri Jun 10 15:23:59 2016 17, 36 -- Received: from IronEB2.johnshopkins.edu (ironeb2.johnshopkins.edu [162.129.199.120]) 17, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5AKNwTE014255 17, 36 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2016 15:23:58 -0500 17, 36 -- X-IronPort-AV: E=Sophos;i="5.26,451,1459828800"; 17, 36 -- d="scan'208";a="323954195" 17, 36 -- Received: from esgebex11.win.ad.jhu.edu ([10.15.89.16]) 17, 36 -- by IronEB2.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 10 Jun 2016 16:24:54 -0400 17, 36 -- Received: from ESGEBEX1.win.ad.jhu.edu (10.15.89.6) by 17, 36 -- ESGEBEX11.win.ad.jhu.edu (10.15.89.16) with Microsoft SMTP Server (TLS) id 17, 36 -- 15.0.1156.6; Fri, 10 Jun 2016 16:24:54 -0400 17, 36 -- Received: from ESGEBEX1.win.ad.jhu.edu ([fe80::9468:4888:5772:702d]) by 17, 36 -- ESGEBEX1.win.ad.jhu.edu ([fe80::9468:4888:5772:702d%31]) with mapi id 17, 36 -- 15.00.1156.000; Fri, 10 Jun 2016 16:24:54 -0400 17, 36 -- From: KEN Livi {klivi-at-jhu.edu} 17, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 36 -- CC: KEN Livi {klivi-at-jhu.edu} , Ada Simari {asimari-at-jhu.edu} 17, 36 -- Subject: Re: EM rates and overhead 17, 36 -- Thread-Topic: EM rates and overhead 17, 36 -- Thread-Index: AQHRwmmcMfAw98hYRkeU0NI9OXpanZ/jaoQA 17, 36 -- Date: Fri, 10 Jun 2016 20:24:53 +0000 17, 36 -- Message-ID: {6B403DDE-59DD-4EC5-970F-EC2FB6980E9A-at-jhu.edu} 17, 36 -- References: {3A42920C-1EDE-44D5-9E67-29F1CC563C29-at-jhu.edu} 17, 36 -- In-Reply-To: {3A42920C-1EDE-44D5-9E67-29F1CC563C29-at-jhu.edu} 17, 36 -- Accept-Language: en-US 17, 36 -- Content-Language: en-US 17, 36 -- X-MS-Has-Attach: 17, 36 -- X-MS-TNEF-Correlator: 17, 36 -- x-ms-exchange-messagesentrepresentingtype: 1 17, 36 -- x-ms-exchange-transport-fromentityheader: Hosted 17, 36 -- x-originating-ip: [10.181.192.131] 17, 36 -- Content-Type: text/plain; charset="utf-8" 17, 36 -- Content-ID: {48805F70FD6C3E45BF1205165B9338CC-at-exchange.johnshopkins.edu} 17, 36 -- MIME-Version: 1.0 17, 36 -- Content-Transfer-Encoding: 8bit 17, 36 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u5AKNwTE014255 ==============================End of - Headers==============================
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Email: roy.geiss-at-colostate.edu Name: Roy Geiss
Organization: Colorado State University
Title-Subject: [Filtered] reliability of EDS detectors
Message: Would anybody have experience or care to comment on the reliability of SDD EDS detectors v. Si(Li) detectors? Feel free to respond off line. Thank you in advance.
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Research Fellowship available - Monash University, Australia
A research fellowship is available in the development and application of methods to characterise the atomic and electronic structure of nanoparticles using quantitative, aberration-corrected S/TEM and related techniques.
Full details and on-line application form can be found at:
Prof. Joanne Etheridge: joanne.etheridge-at-monash.edu Director, Monash Centre for Electron Microscopy and Professor, Department of Materials Science and Engineering
==============================Original Headers============================== 7, 46 -- From matthew.weyland-at-monash.edu Mon Jun 13 00:42:17 2016 7, 46 -- Received: from mail-pa0-f46.google.com (mail-pa0-f46.google.com [209.85.220.46]) 7, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5D5gHr9000718 7, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jun 2016 00:42:17 -0500 7, 46 -- Received: by mail-pa0-f46.google.com with SMTP id bz2so42292589pad.1 7, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 12 Jun 2016 22:43:23 -0700 (PDT) 7, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 46 -- d=monash-edu.20150623.gappssmtp.com; s=20150623; 7, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version 7, 46 -- :content-transfer-encoding; 7, 46 -- bh=Bh95B9u8d04kCNanGNEFOx+eJXLvaWfL2X4JS4yQ+kY=; 7, 46 -- b=taHGoUpmGXnS2/QK0AQA+Otk9ayyx0GJ93RbZ+IJ2utBzdyc2GaRiooxdIn/xl2j0x 7, 46 -- lIMbYTppP3KdCFkdJI5MLb2yWriVNqlmefHiHi9zjzxPLAWz2PSC+FQKZAVqRxLxHeBQ 7, 46 -- hrdjrWYyZw+wMhtwScQc27ck2XaRRwWHZHFTzmGF0JHjQjlVso7GYEX4ggFyeypNC3JA 7, 46 -- Zt83xpPriflhUlOErCt8jWj+f2qe07yaDVaBFbEV1Sm34eQ+yxMvVXPqgAlxAK4AV1cn 7, 46 -- I6ZruOhJshJHh1nMW4zcPHBUfe9UIVuTnZeYTb/JbA1Z2a3UpUi83lvgbtBEVM4/Gebk 7, 46 -- oi0w== 7, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 46 -- d=1e100.net; s=20130820; 7, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 7, 46 -- :mime-version:content-transfer-encoding; 7, 46 -- bh=Bh95B9u8d04kCNanGNEFOx+eJXLvaWfL2X4JS4yQ+kY=; 7, 46 -- b=EYcxbmQ2zuuuNNmOA86pCVMjeNWIvbPyYGDZpOUBg+tha2uXD08k5BRkdWFshE6j7U 7, 46 -- /qWhDbWy+HzTx8bx6jT+6h0L/zpOwPxvc4hVwrHu9eY9XgYtne9UYL1YC9EwsetRixt4 7, 46 -- 9Krwe2cG89SGVBje0aWd6ASiKG3qLZ0c9O/cb3yibymxHrI6LSWf3dRomARPuqkM3gzL 7, 46 -- QOhaxpWNjQ1XuX82hymbzJkPa/7HRU466TlC5MjUqwqSGaRE/bmwa1U7eu4SggmfcMy6 7, 46 -- 3iMwreAm32HMRaUwIsWkCCgTFoULfO7hh5H3NRltL7DczoXs6WWrkM9Neezp75O+yZXh 7, 46 -- Bz+g== 7, 46 -- X-Gm-Message-State: ALyK8tIpJcLSDKrTm6JJ9DN0hUpedY2RI7UuFgfjDE9tcdtj+S+OEZ0bggJRQeDv1bGEUTqI0J2S6ejk6h3OUBL4QYdWRA7uC4w3niSF+If9JwTeclqlxxussjnVahLOLDZjfao/XedKRO4qXrE1ZnUa6PxXwknuNTKd1VaDsOcmmFKewZpdWakEcd80ET9uJ9rJ 7, 46 -- X-Received: by 10.66.66.42 with SMTP id c10mr19973800pat.119.1465796602059; 7, 46 -- Sun, 12 Jun 2016 22:43:22 -0700 (PDT) 7, 46 -- Received: from [49.127.77.25] (dyn-49-127-77-25.its.monash.edu.au. [49.127.77.25]) 7, 46 -- by smtp.gmail.com with ESMTPSA id a85sm21557314pfj.42.2016.06.12.22.43.20 7, 46 -- for {Microscopy-at-microscopy.com} 7, 46 -- (version=TLSv1/SSLv3 cipher=OTHER); 7, 46 -- Sun, 12 Jun 2016 22:43:21 -0700 (PDT) 7, 46 -- To: Microscopy-at-microscopy.com 7, 46 -- From: Matthew Weyland {matthew.weyland-at-monash.edu} 7, 46 -- Subject: Research Fellowship available - Monash University, Australia 7, 46 -- Message-ID: {16b27e10-6328-082f-0b74-5d623f32864c-at-monash.edu} 7, 46 -- Date: Mon, 13 Jun 2016 15:43:25 +1000 7, 46 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:45.0) Gecko/20100101 7, 46 -- Thunderbird/45.1.1 7, 46 -- MIME-Version: 1.0 7, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed 7, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Basic Ultramicrotomy mini-course on July 18th and 19th, 2016. The course is designed to teach novice users of ultramicrotome operation, trimming and sectioning of biological specimen embedded in epoxy or acrylic resin. No prior experience is required.
The course will include lectures, demonstration and (lots of) hands on practice. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found in our website
An advanced cryo-ultramcrotomy course will be offered in October 17 and 18. For more information, please email coreimaging-at-umaryland.edu or visit our website http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/ .
Thanks.
Best regards,
Ru-ching
Ru-ching Hsia, Ph.D. rhsia-at-umaryland.edu Associate Professor and Director Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
==============================Original Headers============================== 2, 19 -- From hsia627-at-hotmail.com Tue Jun 14 08:37:00 2016 2, 19 -- Received: from BLU004-OMC3S22.hotmail.com (blu004-omc3s22.hotmail.com [65.55.116.97]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5EDb02j007200 2, 19 -- for {microscopy-at-microscopy.com} ; Tue, 14 Jun 2016 08:37:00 -0500 2, 19 -- Received: from BLU174-W30 ([65.55.116.72]) by BLU004-OMC3S22.hotmail.com over TLS secured channel with Microsoft SMTPSVC(7.5.7601.23008); 2, 19 -- Tue, 14 Jun 2016 06:38:10 -0700 2, 19 -- X-TMN: [jQE00DgOXCnETYnyt7bezYiskZC1sxVE] 2, 19 -- X-Originating-Email: [hsia627-at-hotmail.com] 2, 19 -- Message-ID: {BLU174-W30E0C096815829FB2C3C0EEF540-at-phx.gbl} 2, 19 -- From: Ruching hsia {hsia627-at-hotmail.com} 2, 19 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 19 -- Subject: Beginner Ultramicrotomy course at U Maryland Baltimore 2, 19 -- Date: Tue, 14 Jun 2016 09:38:09 -0400 2, 19 -- Importance: Normal 2, 19 -- Content-Type: text/plain; charset="iso-8859-1" 2, 19 -- MIME-Version: 1.0 2, 19 -- X-OriginalArrivalTime: 14 Jun 2016 13:38:10.0599 (UTC) FILETIME=[FDEFF370:01D1C641] 2, 19 -- Content-Transfer-Encoding: 8bit 2, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u5EDb02j007200 ==============================End of - Headers==============================
Greetings! Our Summer Conference went very well, and now we are geared up to start our Fall Conference.
The registration for attendees is now open as well as for exhibitors.
The “Diverse Fields of Study…. Steps Toward Medicine” themed conference will also include a student competition, as well as a whole roster of top notch speakers.
Attendees: Welcome from all educational institutions, including the student and post doc presentation competition.
$99 for first places and $50 for second places.
Information and registration can be found at: http://mrl.illinois.edu/mrl-biological-conference-2016
Exhibitors:
_ Table in our vendor fair for 2 days, 2nd day open to any contact you wish to have come to your exhibit: $250
_ A chance to give a 30 minute presentation, Upload abstracts at registration.
__ I will see with our boss if we can broadcast slides and audio to the vendor hall during the talks.
Information and registration can be found at: http://mrl.illinois.edu/mrl-biological-conference-2016
Please feel free to email me with any questions,
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia
Organization: University of Maryland, Baltimore
Title-Subject: [Filtered] Beginner ultramicrotomy course on July 18 and 19, 2016
Message: Dear Colleagues, We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Basic Ultramicrotomy mini-course on July 18th and 19th, 2016. The course is designed to teach novice users of ultramicrotome operation, trimming and sectioning of biological specimen embedded in epoxy or acrylic resin. No prior experience is required. The course will include lectures, demonstration and (lots of) hands on practice. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found in our website http://www.dental.umaryland.edu/2016basicultramicrotomycourse/
An advanced cryo-ultramcrotomy course will be offered in October 17 and 18.
Please email coreimaging-at-umaryland.edu for any inquiries.
Best regards,
Ru-ching
Ru-ching Hsia, Ph.D. Associate Professor and Director Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
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Title-Subject: [Filtered] GSA session T6 on microanalysis and ore mineralogy
Message: Dear all,
*** apologize for cross-posting ***
We would like to direct your attention to the upcoming Annual GSA meeting in Denver, and especially the session T6. Micro-Analytical Techniques in Ore Deposit Research. Please, consider submitting an abstract before July 12th!
http://www.geosociety.org/meetings/2016/sessions/topical.asp This session addresses recent developments and applications of microanalytical techniques (e.g., SEM, EPMA, QEMSCAN, SIMS, TEM, LA-ICP-MS, atom probe...) to refine our understanding of the geochemical and temporal evolution of mineral deposits, as well as to apply these tools to mineral exploration, mineral resource appraisal, and mining and ore extraction. Submissions are encouraged on the analytical techniques and methodologies themselves, as well as the results of their use in experimental, microanalytical, and field studies.
Invited speakers: - Dr. Alan Koenig (USGS, Denver CO) - Dr. Alexander Gysi (Colorado School of Mines) - Dr. Sean Regan (USGS, Montpelier VT)
This session is co-sponsored by the Society of Economic Geologists, the Microanalysis Society (MAS), and the GSA Mineralogy, Geochemistry, Petrology, and Volcanology Division.
Looking forward to see you in Denver!
Katharina Pfaff (Colorado School of Mines) Julien M. Allaz (University of Colorado Boulder)
============================= Dr. Julien Allaz Electron microprobe manager University of Colorado Boulder Dept. of Geological Sciences UCB 399 2200 Colorado Av. Boulder, CO 80309-0399
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Email: edward.calomeni-at-osumc.edu Name: Ed Calomeni
Organization: Ohio State University Wexner Medical Center
Title-Subject: [Filtered] bar coding/sample tracking
Message: Hi Folks,
Our institution is in the process of implementing a bar coding/sample tracking system. Are there any clinical EM labs out there that use such a system? What types of steps do you track? Have you found the system to be useful? (for what?). What systems do you have? Any information will be greatly appreciated. Currently we use a paper log book to keep track of samples. (Guess I am still old school)
Thanks,
Ed Login Host: 140.254.70.165 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: dkuehn-at-pittstate.edu Name: David Kuehn
Organization: Pittsburg State University
Title-Subject: [Filtered] Amray 1200 C
Message: Does anyone have or know where I can get manuals for the operation and maintenance of an Amray 1200 C SEM?
Thanks,
David Kuehn
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Christmas in June this year-time to replace the sputter coater! As we will have this for another 20 years, and it will be in the hands of a thousand or more inexperienced users coating tens of thousands of primarily biologic samples I am soliciting feedback on potential units. Anyone enamored with a recent unit? Find it particularly easy to use or to teach others to use? Or particularly dislike some aspect of their unit? As an example- my current instrument has been a very solid performer for a loooong time- but I just can't stand the overhanging thickness monitor.
A primary consideration- if I can't get 1 page or less ikea style instructions that 90% of the researchers can understand then it is too complicated!
Anyone using the Leica E200 with experiences to share?
I have been intrigued by the osmium plasma coaters- but they seem not to have taken off outside Japan. Assuming there is a reason why? Sure seems less wasteful that the metal foil magnetron heads even though I don't run FESEM- yet.
Thanks
Scott Whittaker Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Room W150 Washington DC 20013-7012 202-633-0891
==============================Original Headers============================== 12, 28 -- From WHITTAKS-at-si.edu Fri Jun 17 14:56:21 2016 12, 28 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5HJuLqF002123 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2016 14:56:21 -0500 12, 28 -- Received: from SI-MSECHT01.US.SINET.SI.EDU (si-msecht01.us.sinet.si.edu [160.111.49.153]) 12, 28 -- by si-mailout04.si.edu (Postfix) with ESMTP id 6C3CBE6168 12, 28 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2016 15:57:43 -0400 (EDT) 12, 28 -- Received: from SI-MSEDAG04.US.SINET.SI.EDU ([169.254.4.84]) by 12, 28 -- SI-MSECHT01.US.SINET.SI.EDU ([160.111.49.153]) with mapi id 14.03.0279.002; 12, 28 -- Fri, 17 Jun 2016 15:57:43 -0400 12, 28 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 12, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 12, 28 -- Subject: Sputter Coater recommendations non-FESEM 12, 28 -- Thread-Topic: Sputter Coater recommendations non-FESEM 12, 28 -- Thread-Index: AdHIsLMD9EzrkOaBTNGsMqm3OhxU7gAIYNUw 12, 28 -- Date: Fri, 17 Jun 2016 19:57:42 +0000 12, 28 -- Message-ID: {5D4F637E5C599649A11693E3B6EAC6293FB801AC-at-si-msedag04.US.SINET.SI.EDU} 12, 28 -- References: {5D4F637E5C599649A11693E3B6EAC6293FB7F26F-at-si-msedag04.US.SINET.SI.EDU} 12, 28 -- In-Reply-To: {5D4F637E5C599649A11693E3B6EAC6293FB7F26F-at-si-msedag04.US.SINET.SI.EDU} 12, 28 -- Accept-Language: en-US 12, 28 -- Content-Language: en-US 12, 28 -- X-MS-Has-Attach: 12, 28 -- X-MS-TNEF-Correlator: 12, 28 -- x-originating-ip: [160.111.49.170] 12, 28 -- Content-Type: text/plain; charset="iso-8859-1" 12, 28 -- MIME-Version: 1.0 12, 28 -- Content-Transfer-Encoding: 8bit 12, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u5HJuLqF002123 ==============================End of - Headers==============================
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] wrinkles/folds in LR White sections
Message: this subject may have been discussed in this forum before, but I cannot locate anything in the archives. The issue is sometimes when working with LR White sections, there are thin dark black wrinkles or folds over parts of the specimen. The blanks areas of resin do not have them, only over the specimen and mainly in organelles. I have also seen them in epoxy sections over large lipid bodies in specimens. I'd like to know if the cause of this problem has been resolved. Thank you- Login Host: 149.169.172.149 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Alliance Technologies, a contact laboratory in NJ, has an immediate opening for a Sr. Microscopist who will characterize widely diverse samples, including organic and inorganic chemicals, household goods, pharmaceuticals, foods, plastics, etc. Analyst must execute experimental studies and deliver high quality data using a broad range of analytical methods including SEM, EDX, FTIR microscopy, Polarized Light microscopy, Fluorescence, Image analysis. Proficiency with sample preparation methods (sectioning, mounting microtome, etc.) required. Familiarity with GMP/GLP and excellent writing skills. PhD in Analytical Chemistry - 3+ years industrial experience, M.S. - 5+ years industrial experience. Background in Mineralogy a plus. Please email resume, job code, and salary history to jobs-at-alliancetechgroup.com for consideration. [16-LM-EM] Senior Scientist, Microscopy & Microanalysis at Alliance Technologies (NJ). Alliance Technologies is a DEA licensed and FDA registered contract testing laboratory in NJ which offers a wide range of chemical analysis and material testing services to a diverse, international client base. www.alliancetechgroup.com
================== Jonathan Chun, Ph.D. Alliance Technologies 9 Deer Park Dr. Suite B Monmouth Jct., NJ 08852 732-355-1234 www.alliancetechgroup.com
==============================Original Headers============================== 6, 25 -- From jkchun-at-alliancetechgroup.com Fri Jun 24 10:44:45 2016 6, 25 -- Received: from remote.alliancetechgroup.com (50-198-237-17-static.hfc.comcastbusiness.net [50.198.237.17]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5OFijsg015700 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 24 Jun 2016 10:44:45 -0500 6, 25 -- Received: from ALLIANCE-SBS11.alliancetechgroup.lan 6, 25 -- ([fe80::c084:c134:20b3:df75]) by ALLIANCE-SBS11.alliancetechgroup.lan 6, 25 -- ([fe80::c084:c134:20b3:df75%11]) with mapi id 14.03.0301.000; Fri, 24 Jun 6, 25 -- 2016 11:46:31 -0400 6, 25 -- From: Jonathan K Chun {jkchun-at-alliancetechgroup.com} 6, 25 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 25 -- CC: Jonathan Chun {jchun-at-alliancetechgroup.com} 6, 25 -- Subject: SEM - Sr Microscopist Opening at Alliance Technologies (NJ) 6, 25 -- Thread-Topic: SEM - Sr Microscopist Opening at Alliance Technologies (NJ) 6, 25 -- Thread-Index: AdHJ6mxf0FOCsNdkRl+sX52yknDgRwERLMyQ 6, 25 -- Date: Fri, 24 Jun 2016 15:46:31 +0000 6, 25 -- Message-ID: {DA10072AA6712D45A799D147F1C956CC18177A9F-at-ALLIANCE-SBS11.alliancetechgroup.lan} 6, 25 -- Accept-Language: en-US 6, 25 -- Content-Language: en-US 6, 25 -- X-MS-Has-Attach: 6, 25 -- X-MS-TNEF-Correlator: 6, 25 -- x-originating-ip: [192.168.1.58] 6, 25 -- Content-Type: text/plain; charset="us-ascii" 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u5OFijsg015700 ==============================End of - Headers==============================
Could somebody suggest an anti-mCherry antibody that would work in immunocytochemistry on,frozen sections (Tokuyasu)? Rabbit polyclonal preferred, but not critical.
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Email: lavoie-at-uw.edu Name: ellen lavoie
Organization: University of Washington
Title-Subject: [Filtered] Hysitron picoindenter
Message: I'm interested in getting any feedback from either current owners/users or prospective owners/users of the Hysitron Picoindenter TEM holder. I've had an inquiry from a faculty member and honestly I've never heard of the company. Am not necessarily exploring owning one but would like to gather info. Please message me individually at lavoie-at-uw.edu.
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Email: hexi-at-missouri.edu Name: Xiaoqing He
Organization: Electron microscopy core at University of Missouri
We have an old Gatan PIPS 691 bought 24 years ago that we are trying to get back to work as normal. It looks pretty much like the modern PIPS 691 except the knobs that are used to control the incident angles of the guns have no readings indicating the exact angles and the gun being in the top or bottom position. We are told that the guns are fixed and the angles relative to the sample is about 5 degrees. We are wondering if anyone in this community has some experience with upgrading the fixed gun configurations to the adjustable ones. Any feedback is greatly appreciated.
Xiaoqing He Senior Research Specialist at EMC core at University of Missouri
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I have a user who is interested in looking at iron nanoparticles in the TEM. I would like to know what people have done to secure the particles so that they do not end up on my pole pieces.
I've suggested three things, but I wanted to know if there might be better ways or which of these might be the best.
1) A long time ago, Tom Nuhfer told me that they used low mag mode and their GIF to preserve magnetic domains in thin films, but I'm not sure that will be high enough magnification for our researcher.
2) Use two grids of thin SiN films above and below the holey carbon films containing the nanoparticles.
3) Embed the nanoparticles and microtome them.
-Scott
Scott D. Walck, Ph.D. 100 Matte Ln Havre de Grace, MD 21078
S.Walck-at-comcast.net SWalck65-at-gmail.com
(760) 685-2815 (Cell) (443) 502-5723 (Home)
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You can just go into the SmartSEM menu, open the stage tab, and initialise it manually. cheers, Rosemary
Dr Rosemary White CSIRO Agriculture GPO Box 1600 Canberra, ACT 2601 Australia Adjunct Professor, EH Graham Centre, CSU & Research School of Biology, ANU T 61 2 6246 5475 E rosemary.white-at-csiro.au
On 30/05/2016 6:29 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
} dsmrc.bulletin-at-gmail.com
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I have not experienced this exact problem, but I do know that some plastic containers, such as tissue culture water bottles, do indeed deteriorate and can crack, split or break into small shards. I'm pretty sure this happens with age regardless of the fluids stored in the bottles; nor does it seem temperature-dependent. Whether or not this is a common problem, I am not sure.
I used 20ml borosilicate glass vials to store tissues. Through VWR, they are relatively inexpensive and more reliable than plastics.
Best regards, Debra M. Townley One Baylor Plaza, Rm. 125A Houston Tx 77030 713-798-4679
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Dear Larry,
Thanks for your answer. I agree that the image resolution can be measured using other ways as mentioned by you, but actually I want to calculate the rpobe size in this case. I want to take some EELS spectra for a range of different probe sizes, say from 50nm-1nm. It would be very interesting for me to exactly know which probe size I have for my current image or spectra. Can I calculate the probe size from CBED discs in a simpler way? Thanks
Regards
Hasan Ali ________________________________________ X-from: les-at-zsgenetics.com [les-at-zsgenetics.com] Sent: Tuesday, June 07, 2016 5:55 PM To: microscopy.listserver-at-gmail.com Cc: Hasan Ali
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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)
Message: Dear Colleagues,
I am a relatively new user of TEM, getting started with STEM now. As in STEM mode, the resolution depends on probe size, so I am really curious to calculate the probe size for my STEM images. I found a formula to calculate probe diameter using CBED discs in William's book but it requires bragg angle (thetaB) in calculations. Is there any simple way to calculate the probe size?
Thanks
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Some bottles marketed as Nalgene are flouropolymers (FEP) and very resistant to chemicals. Some are LDPE, which can be less so. I would suggest checking into exactly which type of bottles you have. A different polymer might work better. Alternatively, are the bottles exposed to strong solvents or acids? Perhaps within the same cabinet or fume hood? Acids in particular can do severe damage to plastic bottles.
-Jake Jokisaari
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I have had several low density polyethylene bottles (I dont know if they are Nalgene brand or not) that have become brittle and will crack when squeezed. One had had dilute nitric acid in it for a while, one had held acetone, and others were empty on the shelf, but had been previously used. I now check the flexibility of any LDPE bottles that I know are not new.
Jim
James B. Murowchick, Ph.D. Associate ProfessorPrincipal Graduate Advisor and IPhD Coordinator Department of Geosciences University of Missouri-Kansas City 420 Flarsheim Hall 5110 Rockhill Rd Kansas City, MO 64110 816 235-2979
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Dear Ali, Do you want to know the probe size or the image resolution? The resolution can be measured, such as by lattice imaging or from a power spectrum. The resolution is related to - but not identical to - the probe size. The probe shape can be modeled in a program like QSTEM.
Regards, Larry Scipioni
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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)
Message: Dear Colleagues,
I am a relatively new user of TEM, getting started with STEM now. As in STEM mode, the resolution depends on probe size, so I am really curious to calculate the probe size for my STEM images. I found a formula to calculate probe diameter using CBED discs in William's book but it requires bragg angle (thetaB) in calculations. Is there any simple way to calculate the probe size?
Thanks
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Just a word of caution when trying to calculate spot size on a scanning probe system! The resolution should not be taken as a spot size measurement. In order to resolve a structure a number of "spots" are required; a typical figure banded around is 4 spots.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: hasan.ali-at-angstrom.uu.se [mailto:hasan.ali-at-angstrom.uu.se] Sent: 25 June 2016 14:03 To: protrain-at-emcourses.com
Dear Larry,
Thanks for your answer. I agree that the image resolution can be measured using other ways as mentioned by you, but actually I want to calculate the rpobe size in this case. I want to take some EELS spectra for a range of different probe sizes, say from 50nm-1nm. It would be very interesting for me to exactly know which probe size I have for my current image or spectra. Can I calculate the probe size from CBED discs in a simpler way? Thanks
Regards
Hasan Ali ________________________________________ X-from: les-at-zsgenetics.com [les-at-zsgenetics.com] Sent: Tuesday, June 07, 2016 5:55 PM To: microscopy.listserver-at-gmail.com Cc: Hasan Ali
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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)
Message: Dear Colleagues,
I am a relatively new user of TEM, getting started with STEM now. As in STEM mode, the resolution depends on probe size, so I am really curious to calculate the probe size for my STEM images. I found a formula to calculate probe diameter using CBED discs in William's book but it requires bragg angle (thetaB) in calculations. Is there any simple way to calculate the probe size?
Thanks
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The 'probe size' in your instrument will depend on a number of factors. At the nm level (not valid for aberration corrected, or even particularly good for high end FEGTEM's) these can be summarised by the effect of diffraction (convergence angle), spherical aberration (from your lens) and the source limit (gun). For a simple look at all these approximations I suggest checking out Michael and Williams, Journal of Microscopy 147 (3), p289 (1987).
As other responders have suggested, this takes no account of your sample and the broadening of the probe. So this is NOT the resolution. In addition once you get into crystals and aberration corrected instruments this is not any use.
If the concept of measuring your convergence angle via diffraction causes you trouble (it is VERY simple, just use a standard gold specimen..) I would suggest you approach STEM on any quantitative level with caution!
Regards
Matthew
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--
*Dr Matthew Weyland * Associate Professor
*Monash Centre for Electron Microscopy and Department of Materials Science and Engineering * 10 Innovation Walk, Clayton Campus Monash University Clayton VIC 3800 Australia
Dear All, we are in process to building a low humidity (~20% RH) room to conduct cryo-tem. Due to low humidity, we are advised by others to include anti-static flooring. I would like to ask if there is any specification we need to look for anti-static flooring? Some propose one with a static electrical propensity of {2.0kV (EN 1815) and electrical resistance {10^10 ohm,may i know if is this good enough protect both human and instrument?
Best Regards Yee Yan, Tay FACTS, NTU Singapore p/s: Earlier i wrote this email in 3DEM but didn't seem to get a reply. Hope no one find it irritating for me to repost this in the microscopy channel.
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I’m looking for a table of ionization cross sections for different elements to use in some EDS calculations. Can anyone recommend a good book or paper that I might refer to?
Thanks for your help!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
You did not mention the electron beam energy. However, Cedric Powell’s most recent review paper is very good as are most of his papers on the topic..
Use of the Bethe equation for inner-shell ionization by electron impact (2016) Cedric J. Powell, Xavier Llovet and Francesc Salvat
American Institute of Physics (AIP) Journal of Applied Physics Show Details Fulltext Permalink Publication Date: 2016-05-14
Description: We analyzed calculated cross sections for K-, L-, and M-shell ionization by electron impact to determine the energy ranges over which these cross sections are consistent with the Bethe equation for inner-shell ionization. Our analysis was performed with K-shell ionization cross sections for 26 elements, with L-shell ionization cross sections for seven elements, L3-subshell ionization cross sections for Xe, and M-shell ionization cross sections for three elements. The validity (or otherwise) of the Bethe equation could be checked with Fano plots based on a linearized form of the Bethe equation. Our Fano plots, which display theoretical cross sections and available measured cross sections, reveal two linear regions as predicted by de Heer and Inokuti [in Electron Impact Ionization, edited by T. D. Märk and G. H. Dunn, (Springer-Verlag, Vienna, 1985), Chap. 7, pp. 232–276]. For each region, we made linear fits and determined values of the two element-specific Bethe parameters. We found systematic variations of these parameters with atomic number for both the low- and the high-energy linear regions of the Fano plots. We also determined the energy ranges over which the Bethe equation can be used.
Print ISSN: 0021-8979 Electronic ISSN: 1089-7550 Topics: Physics Published by American Institute of Physics (AIP)
Nestor Your Friendly Neighborhood SysOp
} On Jun 27, 2016, at 4:17 PM CDT, steven.spurgeon-at-pnnl.gov wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } I’m looking for a table of ionization cross sections for different elements to use in some EDS calculations. Can anyone recommend a good book or paper that I might refer to? } } Thanks for your help! } } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Physical and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } } ==============================Original Headers============================== } 8, 26 -- From prvs=979623209=steven.spurgeon-at-pnnl.gov Mon Jun 27 16:17:05 2016 } 8, 26 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov [192.101.109.63]) } 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u5RLH4GF032105 } 8, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 27 Jun 2016 16:17:05 -0500 } 8, 26 -- Received: from ex10cashub05.pnnl.gov ([130.20.128.106]) } 8, 26 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 27 Jun 2016 14:19:03 -0700 } 8, 26 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.248]) by EX10CASHUB05.pnnl.gov } 8, 26 -- ([130.20.128.106]) with mapi id 14.03.0248.002; Mon, 27 Jun 2016 14:19:04 } 8, 26 -- -0700 } 8, 26 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 8, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 8, 26 -- Subject: Ionization Cross Sections for EDS } 8, 26 -- Thread-Topic: Ionization Cross Sections for EDS } 8, 26 -- Thread-Index: AQHR0LmH7Z1HLyE8NEaYy0AvOZJRGg== } 8, 26 -- Date: Mon, 27 Jun 2016 21:19:03 +0000 } 8, 26 -- Message-ID: {4926332B-8AA3-4F13-A023-02E9F6CB2B15-at-pnnl.gov} } 8, 26 -- Accept-Language: en-US } 8, 26 -- Content-Language: en-US } 8, 26 -- X-MS-Has-Attach: } 8, 26 -- X-MS-TNEF-Correlator: } 8, 26 -- x-originating-ip: [130.20.128.10] } 8, 26 -- Content-Type: text/plain; charset="utf-8" } 8, 26 -- Content-ID: {C0ECA2EE68235741B1824EB7B8342F46-at-pnnl.gov} } 8, 26 -- MIME-Version: 1.0 } 8, 26 -- Content-Transfer-Encoding: 8bit } 8, 26 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u5RLH4GF032105 } ==============================End of - Headers==============================
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center NanoScience and Technology Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
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Email: ehaller-at-health.usf.edu Name: Edward Haller
Organization: University of South Florida
Title-Subject: [Filtered] Need FEI Morgagni or EM 208 specimen holders
Message: We had a student destroy our last good specimen holder (tip) for our FEI Morgagni TEM in our column last week. We're using some old tips from older TEM's that we had in the lab, but they don't work as well for sample exchange. We're looking for (hopefully a donation) of some usable FEI Morgagni or FEI EM 208 specimen tips, if you have any sitting around and have replaced your TEM.
Edward Haller, Lab Manager University of South Florida Department of Integrative Biology Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 (813)974-2676
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I have had no problems containing small magnetic NP's inbetween 2 amorphous SiN windows. You can get very thin SiN now (10 nm) from various vendors, however, if you are going to double them up make sure you verify the thickness of the Si support frame.
I like 100 micron frames, abit fragile but fit every holders. You can also run into 200 and 300 micron thick frames. Those may cause problems depending upon the depth of the "cup" in your specimen holder.
Nestor You Friendly Neighborhood SysOp
On 6/15/16 7:27 AM CDT, Scott Walck wrote: } I have a user who is interested in looking at iron nanoparticles in the TEM. } I would like to know what people have done to secure the particles so that } they do not end up on my pole pieces. } } I've suggested three things, but I wanted to know if there might be better } ways or which of these might be the best. } } 1) A long time ago, Tom Nuhfer told me that they used low mag mode and their } GIF to preserve magnetic domains in thin films, but I'm not sure that will } be high enough magnification for our researcher. } } 2) Use two grids of thin SiN films above and below the holey carbon films } containing the nanoparticles. } } 3) Embed the nanoparticles and microtome them. } } } } -Scott } } Scott D. Walck, Ph.D. } 100 Matte Ln } Havre de Grace, MD 21078 } } S.Walck-at-comcast.net } SWalck65-at-gmail.com } } (760) 685-2815 (Cell) } (443) 502-5723 (Home) } } }
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I have looked at iron nano-particles introduced into cells in culture. I processed the culture in my normal way and embedded in Spurr's Low Viscosity resin, with extra-long in filtration times. I had no trouble with the FE2++ particles staying in place during the cutting of 60nm sections and got good images on the TEM. I will say that cutting iron particles on a diamond knife will leave you with some new scratch marks on your knife :(
Debra M. Townley One Baylor Plaza, Rm. 125A Houston Tx 77030 713-798-4679
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, June 25, 2016 8:48 AM To: Townley, Debra
X-from: adam.fries-at-ucsf.edu
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Email: adam.fries-at-ucsf.edu Name: Adam Fries
Organization: UCSF
Title-Subject: [Filtered] SEM mouse skin images appear melted
Message: Hello All,
I've been working with a researcher that follows the following generic protocol to prep their mouse skin sample:
Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)
1% OsO4 in water 60min Rinse in water 5 min
ethanol 35% - 100% in steps
CPD with LCO2
and sputter coating
Problem: when imaging, some of their samples turn out great, a little less than half look melted and 'blobby'. using the same magnification, probe current, etc.
They have noticed that once out the sputter coater, all specimens appear the same. However, after sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's happening somewhere in the prep, just can't track it down.
Thanks! Adam Login Host: 128.218.238.131 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: adam.fries-at-ucsf.edu Name: Adam Fries
Organization: UCSF
Title-Subject: [Filtered] SEM mouse skin images appear melted
Message: Hello All,
I've been working with a researcher that follows the following generic protocol to prep their mouse skin sample:
Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)
1% OsO4 in water 60min Rinse in water 5 min
ethanol 35% - 100% in steps
CPD with LCO2
and sputter coating
Problem: when imaging, some of their samples turn out great, a little less than half look melted and 'blobby'. using the same magnification, probe current, etc.
They have noticed that once out the sputter coater, all specimens appear the same. However, after sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's happening somewhere in the prep, just can't track it down.
Thanks! Adam Login Host: 128.218.238.131 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Adam, Do you have an image you can share? How are you mounting the specimens? Too much adhesive (cyanoacrylate) can ride up the sides and top surface of small tissue pieces covering them to give a "melted" appearance. The only other thing I am leery about is unbuffered osmium (after primary fixation buffer rinses). Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, June 28, 2016 8:38 PM To: mdelann1-at-jhmi.edu
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Email: adam.fries-at-ucsf.edu Name: Adam Fries
Organization: UCSF
Title-Subject: [Filtered] SEM mouse skin images appear melted
Message: Hello All,
I've been working with a researcher that follows the following generic protocol to prep their mouse skin sample:
Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)
1% OsO4 in water 60min Rinse in water 5 min
ethanol 35% - 100% in steps
CPD with LCO2
and sputter coating
Problem: when imaging, some of their samples turn out great, a little less than half look melted and 'blobby'. using the same magnification, probe current, etc.
They have noticed that once out the sputter coater, all specimens appear the same. However, after sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's happening somewhere in the prep, just can't track it down.
Thanks! Adam Login Host: 128.218.238.131 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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First, how thick and consistent are the skin preps? How much of the dermis is included? Skin is mostly protein, keratin in particular in the outer layers, which does not take up much osmium. Dermis will contain more or less fat, which will take up osmium (especially if the fat has double-bonded carbon) and turn black. But, they'll be black coming out of the osmium. Maybe wash longer. Also: what PO4 buffer? Na/Na2 or Na/K2? Or ... ? If you're going to use osmium, avoid K and use Sorenson's phosphate (Na/Na2), you'll have less trouble with precipitate formation. Or use Na-cacodylate buffer.
Also, try some samples without the osmium post-fix. It imparts some conductivity, but probably isn't needed for this study otherwise.
Further, the fat in the samples may be more or less extracted by the dehydration procedure. How long in each ethanol step? What steps? Don't skip the 95% step (i.e. don't go 90 =} 100%).
Variations in fat content is the most likely reason for the differences in how blackened the samples are.
But. Samples turning color in storage ... that's different. How exactly are the samples stored? When samples turn color, do all the samples in that container turn color, or just a few?
I assume you're looking at the surface of the skin samples. Try taking some "good" ones and some "abnormal" ones (hey, right now, you don't really know which are the real ones and which are artifactual) - the dried samples, post storage, post "huh? they've turned black", and throw them in liquid nitrogen. Fracture them and VERY QUICKLY put them in a desiccator (maybe prefilled with dry N2 from a tank). You *don't* want water condensing on them. Be magical - in liquid nitrogen to in desiccator without seeing the air. Look at the cross-section of the samples - what are the differences? This may help diagnose the problem. If nothing else, they'll be nifty to look at.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
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Email: adam.fries-at-ucsf.edu Name: Adam Fries
Organization: UCSF
Title-Subject: [Filtered] SEM mouse skin images appear melted
Message: Hello All,
I've been working with a researcher that follows the following generic protocol to prep their mouse skin sample:
Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)
1% OsO4 in water 60min Rinse in water 5 min
ethanol 35% - 100% in steps
CPD with LCO2
and sputter coating
Problem: when imaging, some of their samples turn out great, a little less than half look melted and 'blobby'. using the same magnification, probe current, etc.
They have noticed that once out the sputter coater, all specimens appear the same. However, after sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's happening somewhere in the prep, just can't track it down.
Thanks! Adam
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Hello everyone, We have a positon open here at the Johns Hopkins School of Medicine Microscopy Facility for a Microscopy Facility Assistant (Requisition #: 309189). We seek a person talented in both electron microscopy, particularly sample preparation and ultrathin sectioning, and fluorescence confocal microscopy. All interested candidates should apply on-line at :
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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert
Organization: University of Limerick
Title-Subject: [Filtered] Post in Transmission Electron Microscopy
Message: Applications are invited for the following position in the Faculty of Science + Engineering, Department of Physics & Energy, University of Limerick, Ireland:
TITLE OF POST: Lecturer below the bar in Microscopy CONTRACT TYPE: Tenure Track (five year fixed term). During the term of the contract the successful applicant will have the opportunity to apply for tenure in accordance with the University's Policy and Procedures for Granting Multi-annual Status to Entry-level Academic Staff SALARY SCALE: 37,348 - 51,722 p.a. QUALIFICATIONS: A doctoral degree (level 10NFQ) in physics, chemistry, materials science or a related area. OVERALL PURPOSE OF THE JOB: To develop new and complement existing research expertise in electron microscopy, and undertake lecturing and administrative duties at the direction of the Head of Department. DESCRIPTION: The Department of Physics and Energy offers an MSc programme in Applied Physics and undergraduate courses in Applied Physics, Mathematics & Physics, Energy, and Physics education with either Chemistry or Biology. The Department is involved in wide-ranging research and development programmes funded by national and European agencies. In addition to its own modern laboratories, it has direct access to the state-of-the-art facilities of the Bernal Institute. The Bernal Institute represents a multi-million euro investment at the University of Limerick (www.ul.ie) in Science and Engineering (www.scieng.ul.ie), which houses world leading researchers in an appropriately equipped experimental facility. The Department of Physics & Energy is host to the Bernal Chairs in Microscopy and Imaging, and Energy. The successful candidate will be expected to complement and support existing and new, emerging research directions in transmission electron microscopy and must be experienced in the application of state-of-the-art TEM analysis to the characterisation of novel materials for example nano-, bio-, pharma-, and medical materials. ESSENTIAL: A doctoral degree (level 10NFQ) in physics, chemistry, materials science or a related area. Have significant research experience since obtaining their PhD (or equivalent degree) and published in first class journals. The appointee will be capable of lecturing on first and second year general physics and/or chemistry or materials science and in delivering advanced courses in specialized areas of modern measurement. Candidates must have experience in the field of TEM electron tomography and/or holography. DESIRABLE: Experience in X-ray crystallography and X-ray coherent diffractive imaging would be beneficial. The successful candidate will be expected to secure national and European funding and continue to publish in first class journals. The appointee will undertake administrative and promotional roles within the Department and Faculty as and when required. Applicants must demonstrate how their research and teaching would complement existing strengths at the University of Limerick.
Further information for applicants and application material is available online from: http://www.ul.ie/hrvacancies/ The closing date for receipt of applications via online completion is Friday, 22nd July 2016, by 12 noon, Irish Standard Time.
Please email erecruitment-at-ul.ie if you experience any difficulties; queries can also be directed to Ursel Bangert, Bernal Chair in Microscopy and Imaging, Ursel.Bangert-at-ul.ie
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Is there an easy way to add a color scale to images in Digital Micrograph (GMS 3 in particular)? I have some results from a GPA plugin represented as a color map, but no way to produce a scale bar for that color map.
Thank you! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
Dear All, I’m helping a lab convert an old Nikon microscope to DSLR. It has the tube and shutter from a Nikon UFX/HFX film camera system but the controller was apparently surplused years ago. Does anyone know the pins and voltage for the HFX shutter so it can be opened? Then I can lock it open and a DSLR attached to the F-mount used by the old film camera.
Thanks, Glen MacDonald Digital Microscopy Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-uw.edu
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Email: dhowd-at-cvpath.org Name: Deborah Howd
Organization: CVPath Institute
Title-Subject: [Filtered] Long term storage of biological SEM samples
Message: If biological SEM samples (dried,mounted & coated) are stored under controlled temperature and humidity, how long would they still be usable? There is the possibility of needing to look at some samples after up to 5 years of storage. Thank you.
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It is a feature that has been requested a few times and Gatan is working on it. You can create a color scale bar with the following procedure: 1) Go to menu} file} new 2) create an image of the type "ramp Y" 3) In the image option, select the same color scheme as your image 4) Copy past the new image into your original image and use the text tool to add min, max or other values.
Regards,
Dr. Stefano Rubino, Soquelec Limited, Canada
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: June 30, 2016 10:35 AM To: Stefano Rubino {stefano-at-soquelec.com}
Hi everyone,
Is there an easy way to add a color scale to images in Digital Micrograph (GMS 3 in particular)? I have some results from a GPA plugin represented as a color map, but no way to produce a scale bar for that color map.
Thank you! ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
I didn't have the opportunity to use the Hysitron Picoindenter for my graduate work, but the University of Illinois Materials Research Laboratory does own one that is open to all academic researchers. The scientist in charge of that instrument is Dr. CQ Chen. I'm sure you could contact him for more information about his experience with the stage: cqchen-at-illinois.edu.
Wishing you continued success in your research work, -Allen Hall Prairie Nanotechnology
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Email: dyel-at-mail.nih.gov Name: Chip Dye
Organization: NIH
Title-Subject: [Filtered] Glycocalyx Protocol for TEM
Message: Hello,
Does anyone have a good protocol, for imaging using TEM, the glycocalyx in lysosomes in mouse brain tissue (example-Purkinje cells)? Thank you very much! Chip Dye Microscopy & Imaging Core NIH/NICHD 35A Convent Drive Bldg 35A, Rm GD931 Bethesda, MD 20892-3751 (301) 496-3627 voice
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We used plastic bottles, some were culture medium bottles, to store heavy metal containing fluids and other trash from EM for years, almost 10 years to be exact. Some solutions were agressive, containing formaldehyde, glutaraldehyde, osmium tetroxyde or diluted acetone/ethanol. We had no Problem at all. (the bottles were placed on trough to avoid spilling of the content in case of cracks, explosion or accident).
Regards, Stephane
-------------------------------------------- On Sat, 6/25/16, debrat-at-bcm.edu {debrat-at-bcm.edu} wrote:
Subject: [Microscopy] RE: viaWWW:exploding Nalgene bottles? To: nizets2-at-yahoo.com Date: Saturday, June 25, 2016, 3:10 PM
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Hello D. Blackburn,
I have not experienced this exact problem, but I do know that some plastic containers, such as tissue culture water bottles, do indeed deteriorate and can crack, split or break into small shards. I'm pretty sure this happens with age regardless of the fluids stored in the bottles; nor does it seem temperature-dependent. Whether or not this is a common problem, I am not sure.
I used 20ml borosilicate glass vials to store tissues. Through VWR, they are relatively inexpensive and more reliable than plastics.
Best regards, Debra M. Townley One Baylor Plaza, Rm. 125A Houston Tx 77030 713-798-4679
Sent: Wednesday, June 08, 2016 7:07 PM To: Townley, Debra Subject: [Microscopy] viaWWW:exploding Nalgene bottles?
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Email: dblackburn2000-at-yahoo.com Name: D Blackburn
Message: We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because they are so resistant to chemicals. However, we've recently realized that the bottles grow very brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others experienced this? Is it a common, well-known problem?
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Email: dennis-at-ridesoft.com Name: Dennis Tillman
Title-Subject: [Filtered] Tin Balls Message: Does anyone have a "recipe" for making Tin Balls on carbon? These are useful as test specimens. We wanted to try our hand at making our own Tin Balls. For examples of Tin Ball test specimens I mean see https://www.tedpella.com/calibration_html/SEM_Resolution_Test_Specimens_Tin_on_Carbon.htm or http://www.canemco.com/product-catalog/calibration-standards/18-products/210-sem-calibration-standards#Tin_on_Carbon_Resolution_Test_Specimen.
Thank you, Dennis Tillman (dennis-at-ridesoft.com)
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Chip, Look at this reference pertaining to reduced osmium and the glycocalyx:
http://www.ncbi.nlm.nih.gov/pubmed/6202662
good luck, Michael Delannoy -----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Sunday, July 03, 2016 12:07 AM To: delannoy-at-jhmi.edu
X-from: dyel-at-mail.nih.gov
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dyel-at-mail.nih.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: dyel-at-mail.nih.gov Name: Chip Dye
Organization: NIH
Title-Subject: [Filtered] Glycocalyx Protocol for TEM
Message: Hello,
Does anyone have a good protocol, for imaging using TEM, the glycocalyx in lysosomes in mouse brain tissue (example-Purkinje cells)? Thank you very much! Chip Dye Microscopy & Imaging Core NIH/NICHD 35A Convent Drive Bldg 35A, Rm GD931 Bethesda, MD 20892-3751 (301) 496-3627 voice
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Have your thought of polystyrene latex, lots of sizes and as a fluid cheap compared with tin balls? Gives you lots of options for making your own test specimens.
Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
---------------------------------------------------------------------------- ----- Email: dennis-at-ridesoft.com Name: Dennis Tillman
Title-Subject: [Filtered] Tin Balls Message: Does anyone have a "recipe" for making Tin Balls on carbon? These are useful as test specimens. We wanted to try our hand at making our own Tin Balls. For examples of Tin Ball test specimens I mean see https://www.tedpella.com/calibration_html/SEM_Resolution_Test_Specimens_Tin_ on_Carbon.htm or http://www.canemco.com/product-catalog/calibration-standards/18-products/210 -sem-calibration-standards#Tin_on_Carbon_Resolution_Test_Specimen.
Thank you, Dennis Tillman (dennis-at-ridesoft.com)
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A long while back (about a year) I got some very useful advise from you guys regarding the mounting of Trichomonas samples for SEM. Thank you. I found that applying glut fixed cells to a poly-L-lysine (or superfrost+) coated glass slide for 10 minutes, followed by standard SEM processing through osmium, ethanol and HMDS was fine for visualisation of the cells for SEM.
However, while the control strain (T.tenax) processed through perfectly, the test sample (unknown species of dog saliva trichomonas protists) has been a nightmare. The samples can be cultured in variety of media types, protozoan specific media and DMEM based growth medium. pH 8.5 or pH 7.2 seems to have little difference in growth rates, and although they apparently prefer a pH of 8.5, they survive adequately at a standard 7.2.
Initially, we processed the samples through 2.5%GA in DPBS PH7.2, washed 1% aqueous osmium, embedded in agar gel, then dehydrated through an ethanol series before embedding in Spurr's resin, or processed for SEM as described above. The samples weren't great - poor ultrastructure and crenelated cell membrane for the TEM, perforated membranes and collapsed appearance for the SEM, but at least they were intact.
We have since tried:
- the same process, but with a buffer pH of 8.4 (samples looked worse) - initial fix in 0.5%GA followed by the same TEM embedding/SEM dehydration procedure but using buffered osmium (worse still) - initial fix in Bouins fixative followed by the same procedures with buffered osmium (even worse again, as you'd expect) - 2.5% or 5%GA in Millonigs buffer, buffered osmium at 4oC throughout (even worse) - 2%GA/2%osmium mixture in phosphate buffer, followed by 1% osmium and usual processing all at 4oC (really really bad - not a single intact cell to be found) - We've also tried critically point drying the cells for SEM with no discernible difference - the problem clearly lies before this step
It's quite depressing. I feel I'm probably missing something really obvious. The cells that we have observed seem to be collapsed (or sometimes inflated like a balloon), with very poorly intact membranes, and strange lamellar bodies inside the cell with no recognisable organelles (I'm assuming the lamellar bodies are what's remaining of the ultrastructural membranes). I thought it must be the glut affecting the lipid membranes, or maybe some osmotic or ion pump failure after the initial glut fix, but before the osmium stabilised the membranes ( hence the Millonigs buffer and the glut/osmium mixture in the final attempt), but every attempt seems to generate results worse than the last. Sometimes I have received the sample with no apparent cells at all, where I think that maybe the cells have disintegrated completely before reaching the EM lab. I might consider that it's the osmium causing the problems, apart from the fact that the cells have sometimes been clearly damaged before osmium exposure.
Does anyone have any suggestions of an alternative fixation protocol that may stabilise the cells? Is tannic acid our next option. Higher concentrations of sucrose?
Any suggestions are welcome!
TIA, and sorry for the long email.
Natalie
Miss Natalie Allcock EM Facility Manager Electron Microscopy Facility, Centre for Core Biotechnology Services, University of Leicester, University Road, Leicester, LE1 7RH, UK
==============================Original Headers============================== 13, 30 -- From nsa2-at-leicester.ac.uk Tue Jul 5 12:25:36 2016 13, 30 -- Received: from gemsbok.le.ac.uk (gemsbok.le.ac.uk [143.210.16.37]) 13, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u65HPZQB028745 13, 30 -- for {Microscopy-at-Microscopy.com} ; Tue, 5 Jul 2016 12:25:36 -0500 13, 30 -- Received: from exp-casht-i3.le.ac.uk ([143.210.133.195]:37604 helo=exp-casht-i3.uol.le.ac.uk) 13, 30 -- by gemsbok.le.ac.uk with esmtps (TLS1.0:ECDHE_RSA_AES_256_CBC_SHA1:256) 13, 30 -- (Exim 4.84_2) 13, 30 -- (envelope-from {nsa2-at-leicester.ac.uk} ) 13, 30 -- id 1bKU8i-0000LY-3i 13, 30 -- for Microscopy-at-Microscopy.com; Tue, 05 Jul 2016 18:28:04 +0100 13, 30 -- Received: from EXP-DAG1-N2.uol.le.ac.uk ([::1]) by exp-casht-i3.uol.le.ac.uk 13, 30 -- ([143.210.133.195]) with mapi id 14.03.0294.000; Tue, 5 Jul 2016 18:28:04 13, 30 -- +0100 13, 30 -- From: "Allcock, Natalie S." {nsa2-at-leicester.ac.uk} 13, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 13, 30 -- Subject: EM of trichomonas protists 13, 30 -- Thread-Topic: EM of trichomonas protists 13, 30 -- Thread-Index: AdHW4nYFANraKCCfRbqMyNGBAV/00w== 13, 30 -- Date: Tue, 5 Jul 2016 17:28:02 +0000 13, 30 -- Message-ID: {C31045C47F49444BA76584B3DC6020CE16E94E62-at-exp-dag1-n2.uol.le.ac.uk} 13, 30 -- Accept-Language: en-GB, en-US 13, 30 -- Content-Language: en-US 13, 30 -- X-MS-Has-Attach: 13, 30 -- X-MS-TNEF-Correlator: 13, 30 -- x-originating-ip: [143.210.155.106] 13, 30 -- Content-Type: text/plain; charset="us-ascii" 13, 30 -- MIME-Version: 1.0 13, 30 -- X-UoL-Id: 18dea0fe068e68fa5832aebb1a3a68c1-at-1bKU8i-0000LY-3i-at-gemsbok.le.ac.uk 13, 30 -- Content-Transfer-Encoding: 8bit 13, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u65HPZQB028745 ==============================End of - Headers==============================
The collapsed appearance for SEM and the crenelated cell membrane for TEM suggest that osmolarity of the fixative might be the problem, or at least, part of the problem. The unknown species might just be more sensitive to osmotic pressure.
Here is something you can try:
Place cells in fixative, then check cells with a light microscope right away - IF you see cytoplasm "leaking" out of cells, the osmolarity is terribly wrong. GA tends to have very high osmolarity, so increase the GA concentration does not help. IF that is the case, you might want to start with paraformaldehyde. Once cells are dead and not sensitive to osmotic pressure, then switch to GA. The secondary fixative (osmium) should be less a problem.
Have you tried to use the culture medium as the "buffer" for fixative?
Hope this helps and best luck for your adventure.
Zhaojie
Zhaojie Zhang, Ph.D. Director, Jenkins Microscopy Facility University of Wyoming PHONE: 307-766-3038
-----Original Message----- X-from: nsa2-at-leicester.ac.uk [mailto:nsa2-at-leicester.ac.uk] Sent: Tuesday, July 05, 2016 11:35 AM To: Z.J. Zhang {ZZhang-at-uwyo.edu}
Dear All,
A long while back (about a year) I got some very useful advise from you guys regarding the mounting of Trichomonas samples for SEM. Thank you. I found that applying glut fixed cells to a poly-L-lysine (or superfrost+) coated glass slide for 10 minutes, followed by standard SEM processing through osmium, ethanol and HMDS was fine for visualisation of the cells for SEM.
However, while the control strain (T.tenax) processed through perfectly, the test sample (unknown species of dog saliva trichomonas protists) has been a nightmare. The samples can be cultured in variety of media types, protozoan specific media and DMEM based growth medium. pH 8.5 or pH 7.2 seems to have little difference in growth rates, and although they apparently prefer a pH of 8.5, they survive adequately at a standard 7.2.
Initially, we processed the samples through 2.5%GA in DPBS PH7.2, washed 1% aqueous osmium, embedded in agar gel, then dehydrated through an ethanol series before embedding in Spurr's resin, or processed for SEM as described above. The samples weren't great - poor ultrastructure and crenelated cell membrane for the TEM, perforated membranes and collapsed appearance for the SEM, but at least they were intact.
We have since tried:
- the same process, but with a buffer pH of 8.4 (samples looked worse) - initial fix in 0.5%GA followed by the same TEM embedding/SEM dehydration procedure but using buffered osmium (worse still) - initial fix in Bouins fixative followed by the same procedures with buffered osmium (even worse again, as you'd expect) - 2.5% or 5%GA in Millonigs buffer, buffered osmium at 4oC throughout (even worse) - 2%GA/2%osmium mixture in phosphate buffer, followed by 1% osmium and usual processing all at 4oC (really really bad - not a single intact cell to be found) - We've also tried critically point drying the cells for SEM with no discernible difference - the problem clearly lies before this step
It's quite depressing. I feel I'm probably missing something really obvious. The cells that we have observed seem to be collapsed (or sometimes inflated like a balloon), with very poorly intact membranes, and strange lamellar bodies inside the cell with no recognisable organelles (I'm assuming the lamellar bodies are what's remaining of the ultrastructural membranes). I thought it must be the glut affecting the lipid membranes, or maybe some osmotic or ion pump failure after the initial glut fix, but before the osmium stabilised the membranes ( hence the Millonigs buffer and the glut/osmium mixture in the final attempt), but every attempt seems to generate results worse than the last. Sometimes I have received the sample with no apparent cells at all, where I think that maybe the cells have disintegrated completely before reaching the EM lab. I might consider that it's the osmium causing the problems, apart from the fact that the cells have sometimes been clearly damaged be! fore osmium exposure.
Does anyone have any suggestions of an alternative fixation protocol that may stabilise the cells? Is tannic acid our next option. Higher concentrations of sucrose?
Any suggestions are welcome!
TIA, and sorry for the long email.
Natalie
Miss Natalie Allcock EM Facility Manager Electron Microscopy Facility, Centre for Core Biotechnology Services, University of Leicester, University Road, Leicester, LE1 7RH, UK
==============================Original Headers============================== 13, 30 -- From nsa2-at-leicester.ac.uk Tue Jul 5 12:25:36 2016 13, 30 -- Received: from gemsbok.le.ac.uk (gemsbok.le.ac.uk [143.210.16.37]) 13, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u65HPZQB028745 13, 30 -- for {Microscopy-at-Microscopy.com} ; Tue, 5 Jul 2016 12:25:36 -0500 13, 30 -- Received: from exp-casht-i3.le.ac.uk ([143.210.133.195]:37604 helo=exp-casht-i3.uol.le.ac.uk) 13, 30 -- by gemsbok.le.ac.uk with esmtps (TLS1.0:ECDHE_RSA_AES_256_CBC_SHA1:256) 13, 30 -- (Exim 4.84_2) 13, 30 -- (envelope-from {nsa2-at-leicester.ac.uk} ) 13, 30 -- id 1bKU8i-0000LY-3i 13, 30 -- for Microscopy-at-Microscopy.com; Tue, 05 Jul 2016 18:28:04 +0100 13, 30 -- Received: from EXP-DAG1-N2.uol.le.ac.uk ([::1]) by exp-casht-i3.uol.le.ac.uk 13, 30 -- ([143.210.133.195]) with mapi id 14.03.0294.000; Tue, 5 Jul 2016 18:28:04 13, 30 -- +0100 13, 30 -- From: "Allcock, Natalie S." {nsa2-at-leicester.ac.uk} 13, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 13, 30 -- Subject: EM of trichomonas protists 13, 30 -- Thread-Topic: EM of trichomonas protists 13, 30 -- Thread-Index: AdHW4nYFANraKCCfRbqMyNGBAV/00w== 13, 30 -- Date: Tue, 5 Jul 2016 17:28:02 +0000 13, 30 -- Message-ID: {C31045C47F49444BA76584B3DC6020CE16E94E62-at-exp-dag1-n2.uol.le.ac.uk} 13, 30 -- Accept-Language: en-GB, en-US 13, 30 -- Content-Language: en-US 13, 30 -- X-MS-Has-Attach: 13, 30 -- X-MS-TNEF-Correlator: 13, 30 -- x-originating-ip: [143.210.155.106] 13, 30 -- Content-Type: text/plain; charset="us-ascii" 13, 30 -- MIME-Version: 1.0 13, 30 -- X-UoL-Id: 18dea0fe068e68fa5832aebb1a3a68c1-at-1bKU8i-0000LY-3i-at-gemsbok.le.ac.uk 13, 30 -- Content-Transfer-Encoding: 8bit 13, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u65HPZQB028745 ==============================End of - Headers==============================
Dear listers, It looks like our venerable 30-year old JEOL 2000FX has come to the end of its useful life and we need to replace it. One thing this old machine could do, which I have not been able to do in any modern microscope, is take beautiful dark field images at low magnifications ( {2000x), with perfectly parallel illumination and a large field of view. It seems that most modern microscopes don't even go down to such low magnifications and allow dark field imaging, they just turn off the objective lens for 'low mag' mode. It is impossible to obtain low mag, diffraction contrast images on our 5-year old JEOL 2100 TEM; either the beam is too convergent, turning the diffraction condition into a stripe across the image, or the field of view is too small. (The only solution I have found to date is take lots of images and stick them together with photoshop. Time consuming for 30+ images though!)
So my question is - does anyone make a TEM that can do this any more? If so, which one? Can anyone send me a dark field image taken at 1500x on a modern TEM?
I am hoping I'm just ignorant and there's an easy answer...
Thanks
Richard
Richard Beanland Director, Electron Microscopy University of Warwick, UK
==============================Original Headers============================== 11, 32 -- From contact-at-integrityscientific.com Tue Jul 5 17:25:02 2016 11, 32 -- Received: from mo4-p01-ob.smtp.rzone.de (mo4-p01-ob.smtp.rzone.de [81.169.146.164]) 11, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u65MP130025396 11, 32 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jul 2016 17:25:02 -0500 11, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; t=1467757650; l=1280; 11, 32 -- s=domk; d=integrityscientific.com; 11, 32 -- h=Mime-Version:Content-Transfer-Encoding:To:Date:Content-Type:From: 11, 32 -- Subject; 11, 32 -- bh=ZO3s27S+VZ22Aw9HnPqgCJSOBtNQbIggwITK4ICh8nM=; 11, 32 -- b=K2dHLWS0hZLA2ixDoiFEhlBTWME7SQ4JM5ik0RJXHZ9fEBxV93uzQXzUkleIziJmOvG 11, 32 -- On9loAnl41Sb1V0b5xQQw+rireO1AFzO+Ys+wzXGDIPUERUkX2a8Zlpw1GuwGjfcfcKSf 11, 32 -- CXjF2YTz1a67fIviD1W6vOmeeP6kjTZ42u0= 11, 32 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSdqsy65lHRHJMpVnLknqn+F3io/Sw== 11, 32 -- X-RZG-CLASS-ID: mo01 11, 32 -- Received: from [192.168.1.12] (80-42-49-62.dynamic.dsl.as9105.com [80.42.49.62]) 11, 32 -- by smtp.strato.com (RZmta 38.10 DYNA|AUTH) 11, 32 -- with ESMTPSA id Z04877s65MRUEw8 11, 32 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (curve secp521r1 with 521 ECDH bits, eq. 15360 bits RSA)) 11, 32 -- (Client did not present a certificate) 11, 32 -- for {microscopy-at-microscopy.com} ; 11, 32 -- Wed, 6 Jul 2016 00:27:30 +0200 (CEST) 11, 32 -- Subject: Diffraction contrast TEM 11, 32 -- From: Integrity Scientific Ltd {contact-at-integrityscientific.com} 11, 32 -- Content-Type: text/plain; 11, 32 -- charset=us-ascii 11, 32 -- X-Mailer: iPad Mail (13F69) 11, 32 -- Message-Id: {E346A1C1-8736-43F9-934A-F816B06C3B24-at-integrityscientific.com} 11, 32 -- Date: Tue, 5 Jul 2016 23:27:29 +0100 11, 32 -- To: microscopy-at-microscopy.com 11, 32 -- Mime-Version: 1.0 (1.0) 11, 32 -- Content-Transfer-Encoding: 8bit 11, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u65MP130025396 ==============================End of - Headers==============================
We have a JEOL 2100F TEM connected to a Gatan system running Digital Micrograph (GMS 1.86). The computers are all 32 bit running Windows XP and we are not connected to a network. We converted the system to run at 80 kV for a few weeks and then brought it back up to 200 kV. When we did, the power supply on our GIF was dead and it needs repair. We currently have a loaner. I don't know when it blew and don't know whether it was related to the voltage change. The other thing that we noticed was that the microscope status was slow to update on DM. If we rebooted all of the computers, the status of mag would initially change in about a second. As you use the microscope, changing mag and modes, the update would get slower and slower until it takes several minutes for the parameters to be updated to the correct ones. All modes are slow, TEM, STEM, Diff, GIF, etc. This was not happening when we were at 80 kV, only after we were back up to 200 kV.
The Gatan service engineer was not able to fix it, but he seems to remember something like this happening before. He's checking on it. I have a service call in to JEOL, and they seem to remember something like this happening on another system with an Omega filter. We don't have an Omega filter. Something is hogging the communication line between the two systems.
Does anyone have a clue as to what might be happening?
-Scott Walck
==============================Original Headers============================== 8, 33 -- From s.walck-at-comcast.net Tue Jul 5 23:57:13 2016 8, 33 -- Received: from resqmta-ch2-02v.sys.comcast.net (resqmta-ch2-02v.sys.comcast.net [69.252.207.34]) 8, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u664vDbT003110 8, 33 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jul 2016 23:57:13 -0500 8, 33 -- Received: from resomta-ch2-16v.sys.comcast.net ([69.252.207.112]) 8, 33 -- by resqmta-ch2-02v.sys.comcast.net with SMTP 8, 33 -- id Kew3bEMKjQRyhKew3bd1WM; Wed, 06 Jul 2016 04:59:43 +0000 8, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 8, 33 -- s=q20140121; t=1467781183; 8, 33 -- bh=sRC+OFeomxqk3j5Ld6DokYzALsPxqLYxMjOIlUq0JPw=; 8, 33 -- h=Received:Received:From:To:Subject:Date:Message-ID:MIME-Version: 8, 33 -- Content-Type; 8, 33 -- b=boE9b4Nv/cA2ecMFjgrJKrIBnfk+5av2GdTQfGlvAWdjwozMecTl0QqnpNgLpS072 8, 33 -- Gejigw1qXpfdTfz+1sMbhSEG6tYlTF3kfJoURgUJDzn9bBtO8mK8/PuuvrJwQ5aEct 8, 33 -- WaHqqllK1ywPq8wLyC/rOQIXRI1RKeBPdtAndM2Zpwp6rm0qF+mH83YTrbqMxaSt07 8, 33 -- ZFxWOfTp3tgtUoB3aNPS2D/joO+erjI63Uktdqdt+UE2vNaecYauOmj2x6sM2e7Rcl 8, 33 -- MI2uumTqNWFSiMRlzJxJrLnabpBgM1tl7XtpTL4GY3TtrqYHLXnBgaKyO3CJX8YpYY 8, 33 -- BElVRP/DP8V1A== 8, 33 -- Received: from WalckPC ([IPv6:2601:140:4000:c2f5:759e:4204:6d9:794a]) 8, 33 -- by resomta-ch2-16v.sys.comcast.net with comcast 8, 33 -- id FGzj1t0043dJH1s01GzjtL; Wed, 06 Jul 2016 04:59:43 +0000 8, 33 -- From: "Scott Walck" {S.Walck-at-comcast.net} 8, 33 -- To: {microscopy-at-microscopy.com} 8, 33 -- Subject: Slow down in communication between TEM and Digital Micrograph 8, 33 -- Date: Wed, 6 Jul 2016 00:59:42 -0400 8, 33 -- Message-ID: {002501d1d743$35845260$a08cf720$-at-comcast.net} 8, 33 -- MIME-Version: 1.0 8, 33 -- Content-Type: text/plain; 8, 33 -- charset="us-ascii" 8, 33 -- Content-Transfer-Encoding: 7bit 8, 33 -- X-Mailer: Microsoft Outlook 14.0 8, 33 -- thread-index: AdHXQNgHtRDgdi16QG6kQs9hMY3TgQ== 8, 33 -- Content-Language: en-us ==============================End of - Headers==============================
I had a similar issue once on a (non-JEOL) SEM running Gatan DM, and it turned out to be a corrupted driver in the Gatan computer. Deleting and reinstalling the driver fixed the problem.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
________________________________________ X-from: S.Walck-at-comcast.net {S.Walck-at-comcast.net} Sent: Wednesday, July 6, 2016 01:17 To: Oshel, Philip Eugene
Dear Listers,
We have a JEOL 2100F TEM connected to a Gatan system running Digital Micrograph (GMS 1.86). The computers are all 32 bit running Windows XP and we are not connected to a network. We converted the system to run at 80 kV for a few weeks and then brought it back up to 200 kV. When we did, the power supply on our GIF was dead and it needs repair. We currently have a loaner. I don't know when it blew and don't know whether it was related to the voltage change. The other thing that we noticed was that the microscope status was slow to update on DM. If we rebooted all of the computers, the status of mag would initially change in about a second. As you use the microscope, changing mag and modes, the update would get slower and slower until it takes several minutes for the parameters to be updated to the correct ones. All modes are slow, TEM, STEM, Diff, GIF, etc. This was not happening when we were at 80 kV, only after we were back up to 200 kV.
The Gatan service engineer was not able to fix it, but he seems to remember something like this happening before. He's checking on it. I have a service call in to JEOL, and they seem to remember something like this happening on another system with an Omega filter. We don't have an Omega filter. Something is hogging the communication line between the two systems.
Does anyone have a clue as to what might be happening?
-Scott Walck
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Richard; It’s resolution! That’s the spec that sells, and that is what they design and build for. Pole piece designs for higher resolution tends to have smaller gaps, and then to make up for field of view loss due the smaller gaps, they have added “mini-lenses” before and after the OL. On top of that, the OL aperture is not in the back focal plane, but in a hole drilled in the lower half of the OL pole piece. These new microscopes can’t do decent dark field at any magnification, in my experience, but that is not what sells, so say adios to that capability.
John Mardinly, Retired
} On Jul 5, 2016, at 3:42 PM, contact-at-integrityscientific.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } It looks like our venerable 30-year old JEOL 2000FX has come to the end of its useful life and we need to replace it. } One thing this old machine could do, which I have not been able to do in any modern microscope, is take beautiful dark field images at low magnifications ( {2000x), with perfectly parallel illumination and a large field of view. } It seems that most modern microscopes don't even go down to such low magnifications and allow dark field imaging, they just turn off the objective lens for 'low mag' mode. It is impossible to obtain low mag, diffraction contrast images on our 5-year old JEOL 2100 TEM; either the beam is too convergent, turning the diffraction condition into a stripe across the image, or the field of view is too small. (The only solution I have found to date is take lots of images and stick them together with photoshop. Time consuming for 30+ images though!) } } So my question is - does anyone make a TEM that can do this any more? If so, which one? Can anyone send me a dark field image taken at 1500x on a modern TEM? } } I am hoping I'm just ignorant and there's an easy answer... } } Thanks } } Richard } } } } Richard Beanland } Director, Electron Microscopy } University of Warwick, UK } } } } } ==============================Original Headers============================== } 11, 32 -- From contact-at-integrityscientific.com Tue Jul 5 17:25:02 2016 } 11, 32 -- Received: from mo4-p01-ob.smtp.rzone.de (mo4-p01-ob.smtp.rzone.de [81.169.146.164]) } 11, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u65MP130025396 } 11, 32 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jul 2016 17:25:02 -0500 } 11, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; t=1467757650; l=1280; } 11, 32 -- s=domk; d=integrityscientific.com; } 11, 32 -- h=Mime-Version:Content-Transfer-Encoding:To:Date:Content-Type:From: } 11, 32 -- Subject; } 11, 32 -- bh=ZO3s27S+VZ22Aw9HnPqgCJSOBtNQbIggwITK4ICh8nM=; } 11, 32 -- b=K2dHLWS0hZLA2ixDoiFEhlBTWME7SQ4JM5ik0RJXHZ9fEBxV93uzQXzUkleIziJmOvG } 11, 32 -- On9loAnl41Sb1V0b5xQQw+rireO1AFzO+Ys+wzXGDIPUERUkX2a8Zlpw1GuwGjfcfcKSf } 11, 32 -- CXjF2YTz1a67fIviD1W6vOmeeP6kjTZ42u0= } 11, 32 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSdqsy65lHRHJMpVnLknqn+F3io/Sw== } 11, 32 -- X-RZG-CLASS-ID: mo01 } 11, 32 -- Received: from [192.168.1.12] (80-42-49-62.dynamic.dsl.as9105.com [80.42.49.62]) } 11, 32 -- by smtp.strato.com (RZmta 38.10 DYNA|AUTH) } 11, 32 -- with ESMTPSA id Z04877s65MRUEw8 } 11, 32 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (curve secp521r1 with 521 ECDH bits, eq. 15360 bits RSA)) } 11, 32 -- (Client did not present a certificate) } 11, 32 -- for {microscopy-at-microscopy.com} ; } 11, 32 -- Wed, 6 Jul 2016 00:27:30 +0200 (CEST) } 11, 32 -- Subject: Diffraction contrast TEM } 11, 32 -- From: Integrity Scientific Ltd {contact-at-integrityscientific.com} } 11, 32 -- Content-Type: text/plain; } 11, 32 -- charset=us-ascii } 11, 32 -- X-Mailer: iPad Mail (13F69) } 11, 32 -- Message-Id: {E346A1C1-8736-43F9-934A-F816B06C3B24-at-integrityscientific.com} } 11, 32 -- Date: Tue, 5 Jul 2016 23:27:29 +0100 } 11, 32 -- To: microscopy-at-microscopy.com } 11, 32 -- Mime-Version: 1.0 (1.0) } 11, 32 -- Content-Transfer-Encoding: 8bit } 11, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u65MP130025396 } ==============================End of - Headers==============================
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Yale University is looking for a candidate to direct the new CryoEM resource. Please see below for detail.
Thank you for your attention.
Xinran Nick Liu, M.D. & Ph.D. Director of Center for Cellular and Molecular Imaging Bio & Cryo Electron Microscopy Core Facility Yale University School of Medicine Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
-----------------------------------
Director, Yale University Cryo-EM Resource
Continuing a distinguished history of excellence in structural biology, Yale University now seeks a Research Scientist to direct its new University Electron Cryomicroscopy Resource. The Resource will house a Titan Krios microscope with energy filter, direct electron detectors and phase plate; the addition of a Talos Arctica is anticipated in the near future.
The Director will provide scientific leadership, management, and day-to-day direction of the Resource. The incumbent will collaborate with and support researchers from across the campus, many of whom already employ cryo-EM, by providing support and guidance in experimental design, data collection, data quality, and computational analysis as well as assisting in detailed structural analyses of biological macromolecules and complexes.
The Director will report to the offices of the Deputy Provost for Research and the YSM Deputy Dean for Academic and Scientific Affairs, with oversight by an Advisory Committee composed of senior faculty.
The Director should hold a PhD or equivalent degree in biology, chemistry, physics, biophysics, structural biology or a closely related field at the time of hire. The successful candidate should have previous experience in high-resolution structural analysis of macromolecules using cryo-EM including sample preparation and computational analysis with current and customized software; demonstrated competence in setting up, maintaining, and coordinating computational support for cryo-EM data acquisition and analysis; and experience in the optimization and use of current generation electron microscopes such as the Titan Krios.
Interested applicants should submit a CV, cover letter and three professional references to: Cryo-EM Committee, cryo.em-at-yale.edu. Review of applications will begin on August 1, 2016 and continue until the position is filled.
Yale University is an Affirmative Action/Equal Opportunity employer. Yale values diversity among its students, staff, and faculty and strongly welcomes applications from women, persons with disabilities, protected veterans, and underrepresented minorities.
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Hi everyone, I am looking for a third party solution to replace the bellows on a SDD EDX detector. Suggestions, experiences or contact from interested vendors welcome
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
==============================Original Headers============================== 5, 34 -- From gilpin-at-purdue.edu Fri Jul 8 10:14:28 2016 5, 34 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 5, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u68FESn6007168 5, 34 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jul 2016 10:14:28 -0500 5, 34 -- Received: from exchange.purdue.edu (exchange.purdue.edu [128.210.1.29]) 5, 34 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id u68FH7xl001733 5, 34 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 5, 34 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jul 2016 11:17:07 -0400 5, 34 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc15.purdue.lcl 5, 34 -- (172.30.136.188) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Fri, 8 Jul 5, 34 -- 2016 11:17:07 -0400 5, 34 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 5, 34 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 5, 34 -- 15.00.1178.000; Fri, 8 Jul 2016 11:17:07 -0400 5, 34 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 5, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 34 -- Subject: SDD EDX bellows replacement 5, 34 -- Thread-Topic: SDD EDX bellows replacement 5, 34 -- Thread-Index: AdHZKybRGUKCPOB/ReiDgI5fXSoPlQ== 5, 34 -- Date: Fri, 8 Jul 2016 15:17:06 +0000 5, 34 -- Message-ID: {1cc54142f06743e3915021ad3c5d74c8-at-wppexc03.purdue.lcl} 5, 34 -- Accept-Language: en-US 5, 34 -- Content-Language: en-US 5, 34 -- X-MS-Has-Attach: 5, 34 -- X-MS-TNEF-Correlator: 5, 34 -- x-ms-exchange-transport-fromentityheader: Hosted 5, 34 -- x-originating-ip: [10.163.23.192] 5, 34 -- Content-Type: text/plain; charset="utf-8" 5, 34 -- MIME-Version: 1.0 5, 34 -- X-PMX-Version: 6.0.2.2308539 5, 34 -- X-PerlMx-URL-Scanned: Yes 5, 34 -- X-PerlMx-Virus-Scanned: Yes 5, 34 -- Content-Transfer-Encoding: 8bit 5, 34 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u68FESn6007168 ==============================End of - Headers==============================
BMPC's Physical Chemistry team is seeking an experienced Atom Probe Scientist. The selected candidate will perform atom probe research with an emphasis on data collection, data evaluation, and overall experimental interpretation as part of a collaborative team to identify material characteristics, improve fundamental understanding of material behavior, and to support development of improved materials.
Follow link for Job description and information on how to apply:
All candidates must be U.S. citizens. Applicants selected will be subject to a Federal background investigation and must meet eligibility requirements.
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Email: biorlb-at-hofstra.edu. Name: Russ Burke
Organization: Hofstra University
Title-Subject: [Filtered] Electron Microscopy Technician - Job Opening
Message: School/Division: College of Liberal Arts and Sciences
Office: Biology
Full-Time or Part-Time: Part-Time
Description: The Hofstra University Department of Biology is seeks to fill an anticipated Electron Microscopy Technician. The duties of the Electron Microscopy Technician include but are not limited to maintaining and using Hofstra Universitys electron microscope and other advanced scientific equipment, such as Macroscopic Solutions camera system, GPS, particle size analyzer, elemental analyzer (XRF) and field equipment used for teaching and research. He/she will assist faculty with equipment use and perform fixation on samples from any departments (primarily Biology, Chemistry, Geology and Bioengineering) that need require electron micrographs. Preparation of manuals may be required periodically. Assistance in other laboratories doing more general work will occasionally be required.
The technician is expected to work 20 hours/week, although the exact times will vary as needed.
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Conceptually getting rid of the ditch next to the pillar edge is straight-forward. Orient your pillar toward the SEM, and come with FIB from the side at a glancing angle and rotate sample while shaping the pillar.. You would not have any ditch, but instead there will be a pyramid under the pillar. Angle of the pyramid would depend on the angle of pillar relatively to FIB - you can even tilt the sample to a negative angle, if you want the pyramid to be really obtuse. I could dig up a reference on the next week - don't have access to my work computer right now.
Practical implementation will require use of drift correction to maintain relative position of milling box to the center of the pillar, while sample with the pillar is rotated.
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 7/10/2016 4:04 PM, Jason Wang wrote: } Yes, I was using this way. Always having a ditch around the pillar. It's also common in some others pillars but I am thinking to remove it. } } On Jul 10, 2016 3:59 PM, Valery Ray {vray-at-partbeamsystech.com} wrote: } } } } You probably using top-down approach for shaping the pillars? } } } } Valery Ray } } ============================== } } PBS&T, MEO Engineering Company } } 290 Broadway, Suite 298 } } Methuen, MA 01844, USA } } Phone: +1-978-305-0479 - leave a message } } Mobie: +1-978-305-0479 - leave a message } } E-mail: vray-at-partbeamsystech.com } } Web: www.partbeamsystech.com } } Web: www.freudlabs.com } } } } On 7/10/2016 3:49 PM, Jason Wang wrote: } } } Actually not yet, any suggestions? } } } } } } On Jul 10, 2016 3:48 PM, Valery Ray {vray-at-partbeamsystech.com} wrote: } } } } } } Hi Qiang, } } } } } } I am catching up with my mailbox - did you get explanation/reference on } } } micropillars? } } } -- } } } Valery Ray } } } ============================== } } } www.linkedin.com/in/valeryray {http://www.linkedin.com/in/valeryray} } } } PBS&T, MEO Engineering Company } } } 290 Broadway, Suite 298 } } } Methuen, MA 01844, USA } } } Phone: +1-978-305-0479 - leave a message } } } Mobie: +1-978-305-0479 - leave a message } } } E-mail: vray-at-partbeamsystech.com } } } Web: www.partbeamsystech.com {http://www.partbeamsystech.com} } } } Web: www.freudlabs.com {http://www.freudlabs.com} } } }
Hi Scott, we face similar problem where the magnification update becomes very slow (etc takes about 10-50s to update) but we are using DM 2.xx version. What we did is to restart the VME from our TEM side. The VME button is at the back our JEOL 2100F TEM. So just a routine is to shutdown the JEOL PC, Gatan PC, restart VME and start the JEOL PC and subsequently the Gatan PC.
Hope it helps.
Best Regards, YY FACTS NTU
On Wednesday, 6 July 2016, 21:05, "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu} wrote:
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Scott,
I had a similar issue once on a (non-JEOL) SEM running Gatan DM, and it turned out to be a corrupted driver in the Gatan computer. Deleting and reinstalling the driver fixed the problem.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
________________________________________ X-from: S.Walck-at-comcast.net {S.Walck-at-comcast.net} Sent: Wednesday, July 6, 2016 01:17 To: Oshel, Philip Eugene
Dear Listers,
We have a JEOL 2100F TEM connected to a Gatan system running Digital Micrograph (GMS 1.86). The computers are all 32 bit running Windows XP and we are not connected to a network. We converted the system to run at 80 kV for a few weeks and then brought it back up to 200 kV. When we did, the power supply on our GIF was dead and it needs repair. We currently have a loaner. I don't know when it blew and don't know whether it was related to the voltage change. The other thing that we noticed was that the microscope status was slow to update on DM. If we rebooted all of the computers, the status of mag would initially change in about a second. As you use the microscope, changing mag and modes, the update would get slower and slower until it takes several minutes for the parameters to be updated to the correct ones. All modes are slow, TEM, STEM, Diff, GIF, etc. This was not happening when we were at 80 kV, only after we were back up to 200 kV.
The Gatan service engineer was not able to fix it, but he seems to remember something like this happening before. He's checking on it. I have a service call in to JEOL, and they seem to remember something like this happening on another system with an Omega filter. We don't have an Omega filter. Something is hogging the communication line between the two systems.
Does anyone have a clue as to what might be happening?
-Scott Walck
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==============================Original Headers============================== 28, 33 -- From rongchigram79-at-yahoo.com.sg Mon Jul 11 04:32:13 2016 28, 33 -- Received: from nm41-vm1.bullet.mail.gq1.yahoo.com (nm41-vm1.bullet.mail.gq1.yahoo.com [67.195.87.88]) 28, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6B9WC8O030027 28, 33 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2016 04:32:12 -0500 28, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1468229701; bh=9Cs0MGZfuPAMqModv+mEZqDWO5iAe3wlRhQ5C8qR4LY=; h=Date:From:Reply-To:To:In-Reply-To:References:Subject:From:Subject; b=INJnZQT3O6HY2t4UM9rv3jVicrJcISj3oOyK0cPHKnP279oL+AUJXKvMLTj6kZzs6+AXfcfjpxe5fQnvdAk4dXAqxnVMvXwAjSI9ir+EhX3c8cgO8wEpL9NMXF27RKma7t/z3ZnEMrlmBdNZImyxwLRbgymrCVzjamZMXgQudnSnDi5irpoHN/gea43er+P4vQypRmvnZRLBx/Zb+aEcCpWj6H1+At3pX92QsiP/yXtG+8OCRWHmUh7/r8zAubD3yaTcOlormkr1Ng3n6+ZZy33dLT6HyjSQOxULmfymTKbhVmJwaykIk14eaDORBqDQ6pqToRVigyWybWmL9X2fCQ== 28, 33 -- Received: from [127.0.0.1] by nm41.bullet.mail.gq1.yahoo.com with NNFMP; 11 Jul 2016 09:35:01 -0000 28, 33 -- Received: from [98.137.12.56] by nm41.bullet.mail.gq1.yahoo.com with NNFMP; 11 Jul 2016 09:32:17 -0000 28, 33 -- Received: from [106.10.166.123] by tm1.bullet.mail.gq1.yahoo.com with NNFMP; 11 Jul 2016 09:32:16 -0000 28, 33 -- Received: from [106.10.151.170] by tm12.bullet.mail.sg3.yahoo.com with NNFMP; 11 Jul 2016 09:32:16 -0000 28, 33 -- Received: from [127.0.0.1] by omp1010.mail.sg3.yahoo.com with NNFMP; 11 Jul 2016 09:32:16 -0000 28, 33 -- X-Yahoo-Newman-Property: ymail-4 28, 33 -- X-Yahoo-Newman-Id: 370021.87341.bm-at-omp1010.mail.sg3.yahoo.com 28, 33 -- X-YMail-OSG: 0Vj1wfcVM1mS6SgCBe2WpP5EEyxnzTMqDDoY4hddtuyYRkMLQgirvUfOgxFXjfW 28, 33 -- Q4yWMyU5FAGgsu..kpsxwWgexc9BRZCFWmCg0Kqvb2KmZBSGWgN_WV2jAV2VZgPhHbi0rhrP6V39 28, 33 -- o8m3lYDqza5._.twBhsUvva_8Gl6FP6UZYLySEzdD5isVFy_yMda08O4_jVBZUTd26k9vyr8rmac 28, 33 -- KoJk2hCcyDxYcBqv9kuVsft51H1aZES7yiggqtwlKerkdMOKzt28aSEyivmP8IwKoEJmWW6uuR49 28, 33 -- PaSfCPEl97M.gG9lzfICiG0CApe7DujwjBgeqe21TNbxT7o0Czc51OSlX43dafq_MDchbL_PHZQU 28, 33 -- ZtuTaqoSEElD6Ym.MnDilt2MAUDex1R_QokQ_V6Hs_f_qsOJLMmRuaF.BCa_siKfDWMmX5f9wUAJ 28, 33 -- CYG.xUPMoE5l72KBxvapF1vMLM3AeJt1P8ShxB9RbjUBiGrsn0Oa0vnpvO7EiSDUs6dLZJcpg5bQ 28, 33 -- tOsFhdvUj_vV4x1ztww-- 28, 33 -- Received: from jws10909.mail.sg3.yahoo.com by sendmailws114.mail.sg3.yahoo.com; Mon, 11 Jul 2016 09:32:15 +0000; 1468229535.908 28, 33 -- Date: Mon, 11 Jul 2016 09:32:15 +0000 (UTC) 28, 33 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 28, 33 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 28, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 28, 33 -- Message-ID: {1362648128.694431.1468229535525.JavaMail.yahoo-at-mail.yahoo.com} 28, 33 -- In-Reply-To: {201607061302.u66D2voQ012174-at-ns.microscopy.com} 28, 33 -- References: {201607061302.u66D2voQ012174-at-ns.microscopy.com} 28, 33 -- Subject: Re: [Microscopy] Re: Slow down in communication between TEM and 28, 33 -- Digital 28, 33 -- MIME-Version: 1.0 28, 33 -- Content-Type: text/plain; charset=UTF-8 28, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are in need of a used Gatan 795IF CCD controller box for our GIF 200 EELS. If anyone has an old box lying around or you are replacing your old GIF with a new one, please contact me. Thanks, Ken
==============================Original Headers============================== 1, 34 -- From prvs=9934b6f92=klivi-at-jhu.edu Mon Jul 11 11:48:57 2016 1, 34 -- Received: from IronEB2.johnshopkins.edu (ironeb2.johnshopkins.edu [162.129.199.120]) 1, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6BGmv27018054 1, 34 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2016 11:48:57 -0500 1, 34 -- X-IronPort-AV: E=Sophos;i="5.28,347,1464667200"; 1, 34 -- d="scan'208";a="330036252" 1, 34 -- Received: from esgmtwex3.win.ad.jhu.edu ([10.181.25.138]) 1, 34 -- by IronEB2.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 11 Jul 2016 12:51:47 -0400 1, 34 -- Received: from ESGEBEX1.win.ad.jhu.edu (10.15.89.6) by 1, 34 -- ESGMTWEX3.win.ad.jhu.edu (10.181.25.138) with Microsoft SMTP Server (TLS) id 1, 34 -- 15.0.1156.6; Mon, 11 Jul 2016 12:51:47 -0400 1, 34 -- Received: from ESGEBEX1.win.ad.jhu.edu ([fe80::9468:4888:5772:702d]) by 1, 34 -- ESGEBEX1.win.ad.jhu.edu ([fe80::9468:4888:5772:702d%31]) with mapi id 1, 34 -- 15.00.1156.000; Mon, 11 Jul 2016 12:51:46 -0400 1, 34 -- From: KEN Livi {klivi-at-jhu.edu} 1, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 1, 34 -- CC: KEN Livi {klivi-at-jhu.edu} 1, 34 -- Subject: GIF Controller Box 1, 34 -- Thread-Topic: GIF Controller Box 1, 34 -- Thread-Index: AQHR25SCFmPG7qD8Y0G7E0CCtnthvw== 1, 34 -- Date: Mon, 11 Jul 2016 16:51:46 +0000 1, 34 -- Message-ID: {CBCC8278-9093-4284-B933-B691666DC339-at-jhu.edu} 1, 34 -- Accept-Language: en-US 1, 34 -- Content-Language: en-US 1, 34 -- X-MS-Has-Attach: 1, 34 -- X-MS-TNEF-Correlator: 1, 34 -- x-ms-exchange-messagesentrepresentingtype: 1 1, 34 -- x-ms-exchange-transport-fromentityheader: Hosted 1, 34 -- x-originating-ip: [10.181.192.211] 1, 34 -- Content-Type: text/plain; charset="us-ascii" 1, 34 -- Content-ID: {61036DF4539D67429C0D143079F796E6-at-exchange.johnshopkins.edu} 1, 34 -- MIME-Version: 1.0 1, 34 -- Content-Transfer-Encoding: 8bit 1, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u6BGmv27018054 ==============================End of - Headers==============================
This might be a simple question, but is there a way to crop part of an EEL spectrum in Digital Micrograph / GMS 3? It would be great if I could select a region and delete all other parts of the spectrum. Any thoughts?
Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
You can use a ROI to select part of your spectrum and copy/paste it back into the same window. The regions outside the ROI will be cropped (use "show legend" to select and remove the original spectrum).
Regards,
Dr. Stefano Rubino, Sales and Services 604-828-9731 /stefano-at-soquelec.com
Office: 5524 rue Saint Patrick, Suite 405, Montreal, Quebec H4E 1A8, Canada 514-482-6427ext 230/ Fax:514-482-1929/https://www.soquelec.com https://en.wikipedia.org/wiki/Soquelec / https://stefanorubinophd.wordpress.com/
-----Original Message----- X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov] Sent: July 11, 2016 12:20 PM To: Stefano Rubino {stefano-at-soquelec.com}
Hi everyone,
This might be a simple question, but is there a way to crop part of an EEL spectrum in Digital Micrograph / GMS 3? It would be great if I could select a region and delete all other parts of the spectrum. Any thoughts?
Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
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Title-Subject: [Filtered] Position open for Materials Characterization Technician
Message: We have an opening for a FIB/SEM technician, including EDX and 3D reconstruction, at IBM Research-Almaden in San Jose, CA. Applications accepted only through the website.
https://krb-sjobs.brassring.com/TGnewUI/Search/home/HomeWithPreLoad?PageType=JobDetails&partnerid=26059&siteid=5016&jobid=61234&AReq=52775BR&Codes=SmartRecruiters Materials Characterization Technician 52775BR Job Description The Materials characterization team at IBM Almaden Research Center in San Jose, CA has an immediate opening for an experienced SEM/FIB engineer or technician. The work will include SEM imaging, EDX analysis, FIB/TEM preparation, and 3D reconstruction of images using serial acquisition techniques on an FEI Helios 400S Nanolab Dualbeam system. The position is a long-term supplemental (LTS) , and partially off-shift (includes evening hours) with some schedule flexibility as to full or part time. Mechanical sample preparation skills for SEM or TEM is desirable, as well as additional characterization experience (Auger, XPS, SIMS, AFM). US Citizenship is required for this position.
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I would like to draw your attention that a research assistant position at the Electron Microscopy Core Imaging Facility (EMCIF) of University of Maryland Baltimore is currently open for application. If you are interested, please go to the link below for the job description and apply online.
Ru-ching Hsia, Ph.D. rhsia-at-umaryland.edu Associate Professor and Director Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
==============================Original Headers============================== 7, 19 -- From hsia627-at-hotmail.com Tue Jul 12 07:53:06 2016 7, 19 -- Received: from BLU004-OMC3S34.hotmail.com (blu004-omc3s34.hotmail.com [65.55.116.109]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6CCr6ec005238 7, 19 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 07:53:06 -0500 7, 19 -- Received: from BLU174-W17 ([65.55.116.74]) by BLU004-OMC3S34.hotmail.com over TLS secured channel with Microsoft SMTPSVC(7.5.7601.23008); 7, 19 -- Tue, 12 Jul 2016 05:55:59 -0700 7, 19 -- X-TMN: [jhkpbnZMxgTP5Lw8OHA1h68fI9EmswzNUx76PgOHa58=] 7, 19 -- X-Originating-Email: [hsia627-at-hotmail.com] 7, 19 -- Message-ID: {BLU174-W175977208CC306FC425CC2EF300-at-phx.gbl} 7, 19 -- From: Ruching hsia {hsia627-at-hotmail.com} 7, 19 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 19 -- Subject: EM Facility research assistant position open 7, 19 -- Date: Tue, 12 Jul 2016 08:55:59 -0400 7, 19 -- Importance: Normal 7, 19 -- Content-Type: text/plain; charset="iso-8859-1" 7, 19 -- MIME-Version: 1.0 7, 19 -- X-OriginalArrivalTime: 12 Jul 2016 12:55:59.0799 (UTC) FILETIME=[BD078470:01D1DC3C] 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u6CCr6ec005238 ==============================End of - Headers==============================
The Wistar Institute in Philadelphia currently has an opening for a Research Assistant in the Imaging Core Facility: The Imaging Shared Resource at The Wistar Institute is a growing core facility in a dynamic research institution that provides access to standard and advanced optical imaging systems, custom image analysis solutions, individualized training and technical support for a wide range of advanced imaging equipment. The Facility offers a complete soup to nuts approach, offering recommendations on experimental design, best practices for image acquisition, image analysis and data processing, as well as final presentation options. We are currently searching for a dedicated and enthusiastic Research Assistant to join our imaging team to help support cutting-edge cancer research and vaccine development and get the most out of modern imaging methods.
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We are looking for a bachelors or masters level applicant in biological or physical sciences with at least 1 year of experience in a research environment, a passion for learning, helping, and running cutting-edge technology. Experience with fluorescence and luminescence systems is highly desirable, with a multidisciplinary skill set beneficial (cell biology, image analysis, biomedical engineering, biochemistry, life sciences, biophysics and programming). Confidence and experience assisting with small animals (primarily mice) in a barrier facility environment, experience with imaging systems and image analysis, strong computing skills are preferred, experience in fluorescence imaging techniques, and in confocal microscopy are a plus.
Major roles include, but are not limited to: advising, training and assisting researchers in small animal imaging techniques, assistance with widefield and confocal microscopy systems, brightfield and fluorescence microscopy, traditional macro and photographic documentation, assistance with quantitative image analysis, collaboration with researchers to troubleshoot and optimize experimental design, maintaining/calibrating/optimizing imaging equipment, tracking equipment usage data.
The Wistar Institute is located in the University City area of Philadelphia, in the heart of the University of Pennsylvania Campus. Wistar provides resources to its faculty and staff that enable them to conduct cutting edge collaborative research and provides for outstanding intellectual environments and state-of-the-art facilities. Research discoveries conducted at Wistar have led to the development of vaccines; the identification of genes associated with cancers; and the development of many other significant research technologies and tools.
For more information and to apply, please visit our website at www.wistar.org and check in the employment link.
********************************* James Hayden, RBP, FBCA Managing Director, Imaging Shared Resource The Wistar Institute 3601 Spruce St. Philadelphia, PA 19104 (215)898-3887 jhayden-at-wistar.org
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this is just to add a comment about our microscope ... also a JEOL JEM2100F (2012), running the JEOL TEMCON software for controlling, with TVIPS camera and Software, and also SerialEM installed. No Gatan Software / hardware involved (except the Gatan 626 cryo-transfer holder).
Occasionally, - once in a month or two - we face similar problems that on the JEOL PC in TEMCON, execution of "loading the alignment file" can be very very slow (} } 30 sec or much longer), instead of 2 to 5 sec, as it should be. What we do is to restart the PC (not the VME) controlling the JEOL - usually, this is sufficient. Rarely, we also have to re-boot the VME of the JEOL. Then, the routine is to shutdown the TVIPS PC, then the JEOL PC, restart VME and start the JEOL PC and subsequently the TVIPS PC. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: - 16th Europ Microsc Congress EMC http://www.emc2016.fr/en/ 28 Aug - 2 Sept 2016 in Lyon, FR - Microscopy Conference 2017 Dreilaendertagung Lausanne, CH 20-25 August 2017 - next Microbiol. conferences: VAAM/DGHM - Annual Conf March 5-8, 2017, Wuerzburg
==============================Original Headers============================== 7, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Jul 12 12:15:13 2016 7, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6CHFC2l028326 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 12:15:13 -0500 7, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 7, 25 -- by localhost (Postfix) with SMTP id E4BB057792 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 19:18:01 +0200 (CEST) 7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id C44385778B 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 19:18:01 +0200 (CEST) 7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 25 -- with Novell_GroupWise; Tue, 12 Jul 2016 19:18:01 +0200 7, 25 -- Message-Id: {5785426702000054000605A3-at-gwsmtp1.uni-regensburg.de} 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 14.2.1 7, 25 -- Date: Tue, 12 Jul 2016 19:17:59 +0200 7, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 7, 25 -- To: "microscopy server" {Microscopy-at-microscopy.com} 7, 25 -- Subject: Slow down in communication between TEM and 7, 25 -- References: {201607110932.u6B9WpO9030382-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {201607110932.u6B9WpO9030382-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=US-ASCII 7, 25 -- Content-Disposition: inline 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u6CHFC2l028326 ==============================End of - Headers==============================
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Email: ming_j_zhang-at-amat.com Name: Ming Zhang
Organization: Applied Materials Company
Title-Subject: [Filtered] FIB Technician Opening
Message: Applied Materials Company has an immediate opening for an FIB technician in Portland, Oregon.
Applied Materials Company is the world's leading semiconductor equipment manufacturing company and one of top 500 U.S. Companies.
The successful candidate will cover weekend and early night shifts and mainly focus on TEM sample preparations. A minimum of associate degree in Engineering, Materials Science, Chemistry, or Physics, etc. is required. US citizen or green card holder preferred.
Interested individuals should send their resumes to Drew_Goettler-at-amat.com
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Reinhard, Is your computer networked? Is it a Windows system? I experience interruptions with my camera and other hardware drivers when certain automatic updates try to load on my confocal and other microscope computers. For a few years, I had regular "camera not found" messages on one of my research microscopes, and our computer people believe it was certain updates, which disconnect hardware while it installs. I blame Microsoft the most, but never tracked which vendor updates caused the problem. The problem occurs when the update is downloaded, but installation is not complete.
Look for the yellow triangle at the lower right corner of your monitor on the start bar, and reboot whenever you see it. I've had scanning confocal imaging systems stop in the middle of a scan because the update just happened to come at that time.
Your problem may be completely different, since it's an alignment file issue. My problems mostly went away when those equipment computers were taken off the network.
Good luck, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan, MCDB Dept. Ann Arbor, Michigan, USA
On Tue, Jul 12, 2016 at 1:28 PM, {Reinhard.Rachel-at-biologie.uni-regensburg.de} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Colleagues, } } this is just to add a comment about our microscope ... also a JEOL JEM2100F (2012), running the JEOL TEMCON software for controlling, with TVIPS camera and Software, and also SerialEM installed. No Gatan Software / hardware involved (except the Gatan 626 cryo-transfer holder). } } Occasionally, - once in a month or two - we face similar problems that on the JEOL PC in TEMCON, execution of "loading the alignment file" can be very very slow (} } 30 sec or much longer), instead of 2 to 5 sec, as it should be. } What we do is to restart the PC (not the VME) controlling the JEOL - usually, this is sufficient. } Rarely, we also have to re-boot the VME of the JEOL. } Then, the routine is to shutdown the TVIPS PC, then the JEOL PC, restart VME and start the JEOL PC and subsequently the TVIPS PC. } kind regards, } Reinhard } } -- } Prof. Dr. Reinhard Rachel } University of Regensburg } Centre for EM / Anatomy } Faculty of Biology & Preclin. Med. } Universitaetsstrasse 31 } D-93053 Regensburg - Germany } tel +49 941 943 -2837, -1720 } mail reinhard.rachel-at-biologie.uni-regensburg.de } office: VKL 3.1.29 } } Next microscopy conferences: } - 16th Europ Microsc Congress EMC } http://www.emc2016.fr/en/ } 28 Aug - 2 Sept 2016 in Lyon, FR } - Microscopy Conference 2017 } Dreilaendertagung Lausanne, CH } 20-25 August 2017 } - next Microbiol. conferences: } VAAM/DGHM - Annual Conf } March 5-8, 2017, Wuerzburg } } } } } ==============================Original Headers============================== } 7, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Jul 12 12:15:13 2016 } 7, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) } 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6CHFC2l028326 } 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 12:15:13 -0500 } 7, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) } 7, 25 -- by localhost (Postfix) with SMTP id E4BB057792 } 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 19:18:01 +0200 (CEST) } 7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) } 7, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id C44385778B } 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2016 19:18:01 +0200 (CEST) } 7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de } 7, 25 -- with Novell_GroupWise; Tue, 12 Jul 2016 19:18:01 +0200 } 7, 25 -- Message-Id: {5785426702000054000605A3-at-gwsmtp1.uni-regensburg.de} } 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 14.2.1 } 7, 25 -- Date: Tue, 12 Jul 2016 19:17:59 +0200 } 7, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} } 7, 25 -- To: "microscopy server" {Microscopy-at-microscopy.com} } 7, 25 -- Subject: Slow down in communication between TEM and } 7, 25 -- References: {201607110932.u6B9WpO9030382-at-ns.microscopy.com} } 7, 25 -- In-Reply-To: {201607110932.u6B9WpO9030382-at-ns.microscopy.com} } 7, 25 -- Mime-Version: 1.0 } 7, 25 -- Content-Type: text/plain; charset=US-ASCII } 7, 25 -- Content-Disposition: inline } 7, 25 -- Content-Transfer-Encoding: 8bit } 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u6CHFC2l028326 } ==============================End of - Headers==============================
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Email: stlervin-at-yeah.net Name: Ervin
Title-Subject: [Filtered] Is two-photon microscopy maintenance costs high?
Message: Hi! Is anybody knows the mantennace costs of the two-photon microscopy? How about it compared with point scanning confocal? And last one, how long is the lifetime of the laser line generator. Thanks!
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COTA recently instituted a dedicated bus route to/from the airport which goes to the Convention Center and some hotels. Cost is $2.75. You can find details at: http://www.cota.com/Riding-COTA/AirConnect.aspx
Note that they also have a free bus loop that goes along High Street past the Convention Center. It's an easy way to get to nearby restaurants, etc. Again details are at: http://www.cota.com/Riding-COTA/CBUS.aspx
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
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Email: danb-at-alleninstitute.org Name: Dan Bumbarger
Organization: Allen Institute for Brain Science
Title-Subject: [Filtered] Scientist II position available
Message: Our group has a scientist II position available immediately. We are scaling up high throughput transmission electron microscopy techniques to produce petabyte scale datasets of vertebrate cortex synaptic wiring. The advertised position could fill several different roles in our team. We would strongly prefer someone that would be passionate about helping to maximize uptime on our highly customized JEOL microscopes. We strongly prefer experience diagnosing problems and a lack of fear of taking a wrench to the microscopes. Also highly desireable is someone with skills in dealing with the computational and software side of our pipeline. Another type of role that could be filled would be to manage contractor work geared towards modifying the electron optics and other aspects of the system. We are gearing up to do some very big things, it would be a great time to join our team! Please check the Allen Institute website to apply, or contact me directly. Login Host: 207.46.13.116 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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I will be driving to M&M this weekend, and if anyone needs or has a use for negative holders and cartridges for Philips 300/400 and CM series TEMs, I have lots of them. Free to good homes. But ... I have to know by Friday afternoon (the 22nd).
Also, if anyone is still developing negatives, I have a pile of negative hangers.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 67 -- From oshel1pe-at-cmich.edu Wed Jul 20 12:09:18 2016 5, 67 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 67 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6KH9IRw006499 5, 67 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2016 12:09:18 -0500 5, 67 -- Received: from cas.cmich.edu ([141.209.15.90]) 5, 67 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u6KHCept031236 5, 67 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2016 13:12:40 -0400 5, 67 -- Received: from cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) by 5, 67 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 5, 67 -- (TLS) id 14.3.248.2; Wed, 20 Jul 2016 13:12:40 -0400 5, 67 -- Received: from NAM01-SN1-obe.outbound.protection.outlook.com (141.209.2.100) 5, 67 -- by mail.cmich.edu (141.209.15.141) with Microsoft SMTP Server (TLS) id 5, 67 -- 14.3.248.2; Wed, 20 Jul 2016 13:12:40 -0400 5, 67 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 67 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; 5, 67 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 5, 67 -- bh=CjsRYsvjma9CxqKw5qEo7P7ZO5cM/gjxkt1igKaeIwc=; 5, 67 -- b=FRit/sBIDy+Nb/UgHKfNgcg5ACh0pPX4EIKI9AE233INEzzBAqoOYTaVUlaMlyP7B7vvmpcSyH0mfA6Gd3WzjEHLeWv++YokIxk/ffWuKY45q6JK11d6lO2h4MUVPEotDd14Bm+aa4zlkZXqvVFG2X/FoVGE86z4PvRRwd434Pk= 5, 67 -- Received: from CY1PR05MB1996.namprd05.prod.outlook.com (10.162.216.30) by 5, 67 -- CY1PR05MB1994.namprd05.prod.outlook.com (10.162.216.28) with Microsoft SMTP 5, 67 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 5, 67 -- 15.1.549.5; Wed, 20 Jul 2016 17:12:38 +0000 5, 67 -- Received: from CY1PR05MB1996.namprd05.prod.outlook.com ([10.162.216.30]) by 5, 67 -- CY1PR05MB1996.namprd05.prod.outlook.com ([10.162.216.30]) with mapi id 5, 67 -- 15.01.0549.003; Wed, 20 Jul 2016 17:12:37 +0000 5, 67 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 5, 67 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 67 -- Subject: Anyone need film carriers and cartridges for Philips TEMs? 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Email: danb-at-alleninstitute.org Name: Dan Bumbarger
Organization: Allen Institute for Brain Science
Title-Subject: [Filtered] SAP pole piece JEOL 1200
Message: Does anyone have a SAP pole piece that fits a JEOL 1200 TEM? We'd like to do some experiments where we widen the apertures for specimen insertion to further our development of high capacity/high throughput specimen holders. Difficult to find one without purchasing an entire column.. Thanks in advance,
-Dan
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Email: xuanhao.sun-at-uconn.edu Name: Xuanhao Sun
Organization: University of Connecticut
Title-Subject: [Filtered] SEM need help to identify weird things on pollen surface
Message: Hi all,
We need some help from all you veteran microscopists on this listserver to identify some weird structures we saw on the pollen surface, which kinda like twist wrapping candies The pollen grains were dissected out from a closed compartment of the plant before mounting, so these structure should come from inside the plant along with the pollen. Please see the links below. Any suggestion will be appreciated!
In morphology, they look like tiny pollen, which does come in that sort of shape. Forget-me-not pollen is this small. But then the flower was unopened. A mystery.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 E rosemary.white-at-csiro.au
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X-from: xuanhao.sun-at-uconn.edu
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Email: xuanhao.sun-at-uconn.edu Name: Xuanhao Sun
Organization: University of Connecticut
Title-Subject: [Filtered] SEM need help to identify weird things on pollen surface
Message: Hi all,
We need some help from all you veteran microscopists on this listserver to identify some weird structures we saw on the pollen surface, which kinda like twist wrapping candies The pollen grains were dissected out from a closed compartment of the plant before mounting, so these structure should come from inside the plant along with the pollen. Please see the links below. Any suggestion will be appreciated!
We have an opening for a PostDoc / Engineer to support the high-resolution data collection on a Titan Krios and a Polara, using manual and automated operation. Various membrane protein systems are to be imaged in single particle and electron tomography approaches.
Our lab is part of the Biozentrum of the University of Basel in Switzerland, at the border towards Germany and France. The Biozentrum of the University of Basel is one of the leading institutes worldwide for molecular and biomedical basic research and teaching. It is home to more than 30 research groups with scientists from over 40 countries. Research at the Biozentrum focuses on the areas of Cell Growth & Development, Infection Biology, Neurobiology, Structural Biology & Biophysics and Computational & Systems Biology. With its more than 550 employees, the Biozentrum is the largest department at the University of Basel's Faculty of Science.
The Stahlberg group at the Center for Cellular Imaging and NanoAnalytics (C-CINA) of the Biozentrum studies Parkinson's disease and the structure of membrane protein systems by cryo-transmission electron microscopy (EM). Our methods include high-resolution cryo-EM imaging and image processing, to determine the structure of neuronal tissue by electron tomography, or to determine the atomic structure of the investigated ion channels and transporter membrane protein systems. For this purpose, we operate a FEI Titan Krios, equipped with a Gatan GIF-LS and K2-Summit detector, and also a FEI Polara, equipped with another K2 Summit.
To support operation of these two instruments, and to perform manual datacollection and to setup and run automated datacollection, we are looking to recruit a highly motivated PostDoc or Engineer. Your responsibilities are - Vitrification of cryo-EM samples (FEI Vitrobot, Leica GP). - Operation of the Titan Krios in alignment and manual data collection. - Setup and run SerialEM to collect electron tomography dataset and single particle cryo-EM datasets in an automated manner. - Training of students in the above methods. - Optionally: Data processing: Drift correction, particle picking, 3D reconstruction. These steps are partly implemented in an automated pipeline, based on Zorro (Robb McLeod et al.).
Your profile Applicants should have experience in cryo-EM data collection. Knowledge of computer automation of workflows (e.g., SerialEM) is also required. The primary language in the laboratory is English.
The positions are funded for 2 years, an extension is possible. For further details, please contact me offline.
Henning Stahlberg Center for Cellular Imaging and NanoAnalytics (C-CINA) Biozentrum, University of Basel Switzerland Henning.Stahlberg-at-unibas.ch http://c-cina.org
==============================Original Headers============================== 8, 37 -- From henning.stahlberg-at-unibas.ch Mon Jul 25 03:20:28 2016 8, 37 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u6P8KRlk008399 8, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jul 2016 03:20:28 -0500 8, 37 -- Received: from localhost (localhost [127.0.0.1]) 8, 37 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 91AE9220154 8, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jul 2016 10:24:07 +0200 (CEST) 8, 37 -- X-Virus-Scanned: amavisd-new at unibas.ch 8, 37 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 8, 37 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 8, 37 -- with LMTP id 1wM8lVyvaHJJ for {Microscopy-at-microscopy.com} ; 8, 37 -- Mon, 25 Jul 2016 10:24:07 +0200 (CEST) 8, 37 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 8, 37 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 81F62220110 8, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jul 2016 10:24:07 +0200 (CEST) 8, 37 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 8, 37 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::6c18:86f8:9475:4f76%15]) with mapi id 8, 37 -- 14.03.0301.000; Mon, 25 Jul 2016 10:24:07 +0200 8, 37 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 8, 37 -- To: Microscopy {Microscopy-at-microscopy.com} 8, 37 -- Subject: Postdoc / Engineer's position in high-end cryo-EM data collection 8, 37 -- available in Basel, Switzerland 8, 37 -- Thread-Topic: Postdoc / Engineer's position in high-end cryo-EM data 8, 37 -- collection available in Basel, Switzerland 8, 37 -- Thread-Index: AQHR5k3oe/mmEqB3xE+OHuGe6foYng== 8, 37 -- Date: Mon, 25 Jul 2016 08:24:06 +0000 8, 37 -- Message-ID: {09C87E3C-5E0D-495E-AA27-602F6BD3CADF-at-unibas.ch} 8, 37 -- Accept-Language: en-US, de-CH 8, 37 -- Content-Language: en-US 8, 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- x-originating-ip: [131.152.226.242] 8, 37 -- Content-Type: text/plain; charset="us-ascii" 8, 37 -- Content-ID: {9D3EFB23301DBF4B9F0CEB041381738F-at-zuv.ads.unibas.ch} 8, 37 -- MIME-Version: 1.0 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u6P8KRlk008399 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in Message: Hi: Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj. aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj. x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards Login Host: 5.216.254.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 10, 53 -- From microscopy.listserver-at-gmail.com Mon Aug 1 18:47:13 2016 10, 53 -- Received: from mail-it0-f50.google.com (mail-it0-f50.google.com [209.85.214.50]) 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u71NlBmt016867 10, 53 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2016 18:47:11 -0500 10, 53 -- Received: by mail-it0-f50.google.com with SMTP id j124so187994007ith.1 10, 53 -- for {microscopy-at-microscopy.com} ; Mon, 01 Aug 2016 16:51:21 -0700 (PDT) 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; s=20120113; 10, 53 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 10, 53 -- :mime-version:in-reply-to:content-transfer-encoding; 10, 53 -- bh=lDPZ4k0Wks/EejPwoOBV6cJOMEAhZrhDBLthGI826P4=; 10, 53 -- b=dt0i3K1A/G1LuFcra8agEpwMAjSC9ZJypy5bxpPbAClSOQ8f6v+Zj88RX8Soh2OmdE 10, 53 -- zwUFEd0EP/RRQ+GyZYmjBhwMpp+Xop73H9067FTgrJ/y/wQ3Nhp5CJ8eihahr039sp+J 10, 53 -- J/R2ufnjW92+FG+GDRJD5DpdwObs9P9uGOYi+R07Lfd+Sh8Ad73D5X8HGCr59B6IWb2G 10, 53 -- /yTDXUaZ6qZYW+6yuTTHXPamzMNDuSgS67kDC/pAbJLtxm1Ez7HGzut0GG6oDlZAGr3j 10, 53 -- /DLRQVE7zG87MkwlehoXWn23LjtdYCnYEes489O3ZHMJ9xVyO3F9FRe3DcRsUwRzpTMH 10, 53 -- rXiQ== 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; s=20130820; 10, 53 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 10, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 10, 53 -- bh=lDPZ4k0Wks/EejPwoOBV6cJOMEAhZrhDBLthGI826P4=; 10, 53 -- b=SXYS6S+vjR3vZTinB4nJ8LDxtKGLmkpIbRpXSHtF4rHzQ9cTskwmR+no82h146M+Fs 10, 53 -- RNUgLycFFSjDKDgPdzF+Vqc07GIF/rzAe/Hmk3Y6kXQzezgCPH3eoCTjHWOviqWFlFmJ 10, 53 -- MnfqWJ5KJtAU0iBnHWv+yyiIfoMA2Ll8HS1V4WckWQwfbd8G5Cnsw8rNaADZ+FrSNRF+ 10, 53 -- SIVjP99j4Cy+bgAZcTC4B4ulke0zH4t5V+2HfFG3u1EoUribL2+9DheU2eD5cLnMWz6D 10, 53 -- J5jZiOtXR8noa/9qOduqzIEVepTbLg7f0g2rG1d8KMvmxB1eJrXjYx26Mm67+b2auhn+ 10, 53 -- rJhw== 10, 53 -- X-Gm-Message-State: AEkoouuamLqd9ty5KOU0vaRUbYFtyAlzar8Mcr4hg3Rw6a3eQ5fycIDo7PvkVwLIOuDWnA== 10, 53 -- X-Received: by 10.36.87.140 with SMTP id u134mr62579644ita.38.1470095480495; 10, 53 -- Mon, 01 Aug 2016 16:51:20 -0700 (PDT) 10, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([24.15.143.135]) 10, 53 -- by smtp.googlemail.com with ESMTPSA id i80sm118518ita.5.2016.08.01.16.51.19 10, 53 -- for {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 10, 53 -- Mon, 01 Aug 2016 16:51:19 -0700 (PDT) 10, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 10, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply 10, 53 -- {microscopylistserver-noreply-at-microscopy.com} 10, 53 -- Subject: viaWWW: Advice Needed Transmission electron microscope cm-10 10, 53 -- alignment, beam disappear in M magnification range with object aperture in 10, 53 -- References: {201608011529.u71FTD7o025749-at-ns.microscopy.com} 10, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- X-Forwarded-Message-Id: {201608011529.u71FTD7o025749-at-ns.microscopy.com} 10, 53 -- Message-ID: {cb5f9d32-811c-3454-af15-16c4ae5eeb5d-at-microscopy.com} 10, 53 -- Date: Mon, 1 Aug 2016 18:51:18 -0500 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 10, 53 -- Gecko/20100101 Thunderbird/45.2.0 10, 53 -- MIME-Version: 1.0 10, 53 -- In-Reply-To: {201608011529.u71FTD7o025749-at-ns.microscopy.com} 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
With our CM-12 filaments, I sometimes have to take out and readjust new filaments after they have initially been installed. The filament alignment can change too much with initial heating to adjust it with just the shift x,y knobs. Also, I would try manually adjusting the filament a little further back from the aperture, and then using the emission settings to bring it closer as you do alignment. Sometimes wI find our filaments are set a little too close to the aperture.
Crystal Replogle TEM Technical Manager Asbestos TEM Laboratories
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Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in Message: Hi: Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj. aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj. x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards Login Host: 5.216.254.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 19, 33 -- From CReplogle-at-asbestostemlabs.com Tue Aug 2 13:31:23 2016 19, 33 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u72IVLiZ019656 19, 33 -- for {Microscopy-at-Microscopy.com} ; Tue, 2 Aug 2016 13:31:21 -0500 19, 33 -- Received: from [173.11.101.17] (helo=remote.asbestostemlabs.com) 19, 33 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 19, 33 -- (envelope-from {CReplogle-at-asbestostemlabs.com} ) 19, 33 -- id 1bUeXH-0001aQ-UE; Tue, 02 Aug 2016 14:35:28 -0400 19, 33 -- Received: from CHRYS2011.AsbestosTEMLabs.local ([fe80::d359:a127:6f77:ef41]) 19, 33 -- by CHRYS2011.AsbestosTEMLabs.local ([fe80::d359:a127:6f77:ef41%14]) with mapi 19, 33 -- id 14.03.0294.000; Tue, 2 Aug 2016 11:35:21 -0700 19, 33 -- From: Crystal Replogle {CReplogle-at-asbestostemlabs.com} 19, 33 -- To: "researchers4u-at-gmail.com" {researchers4u-at-gmail.com} , 19, 33 -- "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 19, 33 -- Subject: RE: [Microscopy] viaWWW: Advice Needed Transmission electron 19, 33 -- microscope cm-10 19, 33 -- Thread-Topic: [Microscopy] viaWWW: Advice Needed Transmission electron 19, 33 -- microscope cm-10 19, 33 -- Thread-Index: AQHR7IM3gnxvGHj6aEKfSfvwlhPy8KA1+5zw 19, 33 -- Date: Tue, 2 Aug 2016 18:35:20 +0000 19, 33 -- Message-ID: {A7782DA7FCE133448288F03AAC0B9D6286D6257C-at-CHRYS2011.AsbestosTEMLabs.local} 19, 33 -- References: {201608020556.u725uX91019706-at-ns.microscopy.com} 19, 33 -- In-Reply-To: {201608020556.u725uX91019706-at-ns.microscopy.com} 19, 33 -- Accept-Language: en-US 19, 33 -- Content-Language: en-US 19, 33 -- X-MS-Has-Attach: 19, 33 -- X-MS-TNEF-Correlator: 19, 33 -- Content-Type: text/plain; charset="us-ascii" 19, 33 -- MIME-Version: 1.0 19, 33 -- X-ELNK-Trace: ed1fc8fa8d4c3188d780f4a490ca6956527bd5036cbc8ac7620b2f0c83cb9a55681a64e6f951bf60350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 19, 33 -- X-Originating-IP: 173.11.101.17 19, 33 -- Content-Transfer-Encoding: 8bit 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u72IVLiZ019656 ==============================End of - Headers==============================
Also, if you've exhausted all the tricks, the following should definitely work provided there are no failed parts:
1. All the apertures out (they should surely be out); 2. Bottom up, turn off major lenses one by one till you see the beam or a weak flickering spot (needle size); 3. Top down, turn on the lenses one by one, and maximize the brightness at each step.
******************************************************* Chaoying Ni, PhD Director, W. M. Keck Center for Advanced Microscopy and Microanalysis Professor, Department of Materials Science and Engineering http://www.camm.udel.edu; http://www.mseg.udel.edu (302) 831-6359(Phone); (302) 831-4545 (Fax)
Mailing address: University of Delaware 201 duPont Hall, Newark, DE 19716 *******************************************************
-----Original Message----- X-from: CReplogle-at-asbestostemlabs.com [mailto:CReplogle-at-asbestostemlabs.com] Sent: Tuesday, August 2, 2016 2:57 PM To: Ni, Chaoying {cni-at-udel.edu}
With our CM-12 filaments, I sometimes have to take out and readjust new filaments after they have initially been installed. The filament alignment can change too much with initial heating to adjust it with just the shift x,y knobs. Also, I would try manually adjusting the filament a little further back from the aperture, and then using the emission settings to bring it closer as you do alignment. Sometimes wI find our filaments are set a little too close to the aperture.
Crystal Replogle TEM Technical Manager Asbestos TEM Laboratories
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, August 01, 2016 10:57 PM To: Crystal Replogle
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Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in Message: Hi: Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj. aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj. x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards Login Host: 5.216.254.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 19, 33 -- From CReplogle-at-asbestostemlabs.com Tue Aug 2 13:31:23 2016 19, 33 -- Received: from elasmtp-dupuy.atl.sa.earthlink.net (elasmtp-dupuy.atl.sa.earthlink.net [209.86.89.62]) 19, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u72IVLiZ019656 19, 33 -- for {Microscopy-at-Microscopy.com} ; Tue, 2 Aug 2016 13:31:21 -0500 19, 33 -- Received: from [173.11.101.17] (helo=remote.asbestostemlabs.com) 19, 33 -- by elasmtp-dupuy.atl.sa.earthlink.net with esmtpa (Exim 4.67) 19, 33 -- (envelope-from {CReplogle-at-asbestostemlabs.com} ) 19, 33 -- id 1bUeXH-0001aQ-UE; Tue, 02 Aug 2016 14:35:28 -0400 19, 33 -- Received: from CHRYS2011.AsbestosTEMLabs.local ([fe80::d359:a127:6f77:ef41]) 19, 33 -- by CHRYS2011.AsbestosTEMLabs.local ([fe80::d359:a127:6f77:ef41%14]) with mapi 19, 33 -- id 14.03.0294.000; Tue, 2 Aug 2016 11:35:21 -0700 19, 33 -- From: Crystal Replogle {CReplogle-at-asbestostemlabs.com} 19, 33 -- To: "researchers4u-at-gmail.com" {researchers4u-at-gmail.com} , 19, 33 -- "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 19, 33 -- Subject: RE: [Microscopy] viaWWW: Advice Needed Transmission electron 19, 33 -- microscope cm-10 19, 33 -- Thread-Topic: [Microscopy] viaWWW: Advice Needed Transmission electron 19, 33 -- microscope cm-10 19, 33 -- Thread-Index: AQHR7IM3gnxvGHj6aEKfSfvwlhPy8KA1+5zw 19, 33 -- Date: Tue, 2 Aug 2016 18:35:20 +0000 19, 33 -- Message-ID: {A7782DA7FCE133448288F03AAC0B9D6286D6257C-at-CHRYS2011.AsbestosTEMLabs.local} 19, 33 -- References: {201608020556.u725uX91019706-at-ns.microscopy.com} 19, 33 -- In-Reply-To: {201608020556.u725uX91019706-at-ns.microscopy.com} 19, 33 -- Accept-Language: en-US 19, 33 -- Content-Language: en-US 19, 33 -- X-MS-Has-Attach: 19, 33 -- X-MS-TNEF-Correlator: 19, 33 -- Content-Type: text/plain; charset="us-ascii" 19, 33 -- MIME-Version: 1.0 19, 33 -- X-ELNK-Trace: ed1fc8fa8d4c3188d780f4a490ca6956527bd5036cbc8ac7620b2f0c83cb9a55681a64e6f951bf60350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 19, 33 -- X-Originating-IP: 173.11.101.17 19, 33 -- Content-Transfer-Encoding: 8bit 19, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u72IVLiZ019656 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 28 -- From cni-at-udel.edu Tue Aug 2 16:39:42 2016 24, 28 -- Received: from NSS-EXDR03.win.udel.edu (owa.win.udel.edu [128.175.4.102]) 24, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u72LddxP005469 24, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 2 Aug 2016 16:39:39 -0500 24, 28 -- Received: from nss-exdr02.win.udel.edu (128.175.4.101) by 24, 28 -- NSS-EXDR03.win.udel.edu (128.175.4.102) with Microsoft SMTP Server (TLS) id 24, 28 -- 15.1.466.34; Tue, 2 Aug 2016 17:43:44 -0400 24, 28 -- Received: from nss-exdr02.win.udel.edu ([fe80::3d2d:628d:b19c:5185]) by 24, 28 -- nss-exdr02.win.udel.edu ([fe80::3d2d:628d:b19c:5185%13]) with mapi id 24, 28 -- 15.01.0466.034; Tue, 2 Aug 2016 17:43:44 -0400 24, 28 -- From: "Ni, Chaoying" {cni-at-udel.edu} 24, 28 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 24, 28 -- Subject: RE: [Microscopy] RE: viaWWW: Advice Needed Transmission electron 24, 28 -- Thread-Topic: [Microscopy] RE: viaWWW: Advice Needed Transmission electron 24, 28 -- Thread-Index: AQHR7PBNe47DnOc7lkaiN0Uu2SHf06A2MYEQ 24, 28 -- Date: Tue, 2 Aug 2016 21:43:44 +0000 24, 28 -- Message-ID: {eb0d8f24d0164bf6a114bf3ef8a3b44b-at-udel.edu} 24, 28 -- References: {201608021857.u72IvMCU022388-at-ns.microscopy.com} 24, 28 -- In-Reply-To: {201608021857.u72IvMCU022388-at-ns.microscopy.com} 24, 28 -- Accept-Language: en-US 24, 28 -- Content-Language: en-US 24, 28 -- X-MS-Has-Attach: 24, 28 -- X-MS-TNEF-Correlator: 24, 28 -- x-originating-ip: [74.103.155.52] 24, 28 -- Content-Type: text/plain; charset="iso-8859-1" 24, 28 -- MIME-Version: 1.0 24, 28 -- Content-Transfer-Encoding: 8bit 24, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u72LddxP005469 ==============================End of - Headers==============================
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Email: marie.cantino-at-uconn.edu Name: Marie Cantino
Organization: Dr.
Title-Subject: [Filtered] section folds
Message: Does anyone know how to prevent section folds in 1 or 2 micron thick sections of plant stem material embedded in Spurrs resin? The sections are fairly large, about 4 x 7 mm, cut on a histo diamond, then placed on water drops on Fisher Superfrost Plus slides and dried on a slide warmer at about 70 degrees C. We get multiple section folds parallel to the short axis of the rectangle, that start at the resin surrounding the tissue and project into the tissue at the center of the block. Thanks! -Marie
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Simulation of TEM EDS w/DTSA
Message: Greetings,
I have a Faculty member using DTSA to simulate EDS spectra collected with TEM EDS. [using Sim Alien]. Sim samples are silicate minerals as "grain mounts" on a Cu grid [assumed config]. We are working our way through the Preferences set up for instrument, detector, sample. Using the general parameters of my TEM SDD detector [Thermo SDD] and using 100 & 200 kV acc voltages. Question: [ignoring the somewhat steep DTSA learning curve] are there specific issues or problems with using DTSA to simulate TEM EDS spectra? Assuming anyone out there does this kind of thing!
Any sage advice would be appreciated.
Thanks, Tom
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Title-Subject: [Filtered] Embedding gram positive bacteria for sectioning
Message: I had a batch of gram positive bacteria that I embedded in Medium hardness EM Sciences Spurr's resin. I find most bacteria have holes despite long infiltration under vacuum.
Would a softer mix infiltrate better? Or is there a better resin for gram positive bacteria?
thanks
Joe
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Email: gncherr-at-ucdavis.edu Name: Gary Cherr
Organization: UC Davis Bodega Marine Lab
Title-Subject: [Filtered] need Olympus Fluoview 500 controller card
Message: We have an Olympus Fluoview 500 scanning laser confocal microscope running on WinXP. The controller card (2 PCI cards linked together) has failed. Does anybody know where I can purchase one? Olympus is looking but not supported anymore.
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Sorry to see your problems but maybe I can help. After 50 years with these instruments the problem you have I have seen very many times, I hope we can fix it for you? Set out below are the very basic principles for alignment of a TEM.
1. All lenses OFF, all apertures out and specimen out 2. High voltage on, filament heated to a position just short of the position that you would expect to be normal (de saturated source is bigger than a saturated source so often easier to find) 3. With gun alignment shift controls search for the beam. (if this is difficult use a raster motion of stepping one control in small steps and each step take the other control through its full range. 4. Once the beam is found adjust the gun tilt to obtain a maximum signal. 5. Switch on the first condenser lens, set the lens to a large spot size and adjust the gun tilt and shift for a maximum signal. 6. Switch on the second condenser lens and adjust Condenser 2 (often called brightness) to obtain the brightest spot and centre this with you illumination shift controls. 7. Switch on the objective lens and adjust Condenser 2 and the illumination shift controls again. 8. Set the magnification around 2,000X and switch on each of the other imaging lenses. Adjust Condenser 2 and the illumination shift controls to obtain the maximum intensity. 9. Switch to diffraction and obtain the diffraction point, shift this with the last lens in the column to the centre of the screen. This may be a mechanical or deflection coil alignment for image centre or equivalent name. 10. Insert a condenser aperture and with the Condenser 2 at focus and centred, expand the Condenser (clockwise) and adjust the aperture shadow to fit the screen with the aperture controls. Take the Condenser back to crossover and centre with the illumination shift if required, then repeat this section for a constant centre. Always expand the beam by turning the Condenser clockwise. 11. Adjust Condenser 2 to crossover and you will visualise the virtual source. Use the gun alignment tilt to set the "spot and halo" to be even on opposite sides, centre with the gun alignment shift;. Heat the filament until it is ALMOST a solid spot. (The folk law which says you must over saturate the filament slightly became out of date about 30 years ago!) 12. Insert a specimen and see that everything is operating near normal. As you focus you may find the image and the illumination alignment move, this is to be expected so correct the illumination with the illumination shift controls. Check section 10 again. 13. Switch on the objective alignment pulse system (I do not remember what it is called on an EM10), it will make the image pulse to help you find the objective axis centre. Use the illumination alignment tilt to place the centre of the pulse on the centre of the screen, correcting illumination movement with the illumination shift controls. 13. Switch to diffraction and reset the diffraction centre, insert an objective aperture.
I hope I have thought of everything, if not please come back to be.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in Message: Hi: Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj. aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj. x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards Login Host: 5.216.254.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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==============================Original Headers============================== 17, 20 -- From protrain-at-emcourses.com Thu Aug 4 04:52:29 2016 17, 20 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u749qS3O031850 17, 20 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2016 04:52:28 -0500 17, 20 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 17, 20 -- id {B57a3114c0000} ; Thu, 04 Aug 2016 10:56:28 +0100 17, 20 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Thu, 4 Aug 2016 10:56:27 +0100 17, 20 -- From: "Steve Chapman" {protrain-at-emcourses.com} 17, 20 -- To: {'researchers4u-at-gmail.com'} 17, 20 -- Cc: {microscopy-at-microscopy.com} 17, 20 -- Subject: FW: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10 17, 20 -- Date: Thu, 4 Aug 2016 10:56:38 +0100 17, 20 -- Message-ID: {006f01d1ee36$7e3df790$7ab9e6b0$-at-com} 17, 20 -- MIME-Version: 1.0 17, 20 -- Content-Type: text/plain; 17, 20 -- charset="us-ascii" 17, 20 -- Content-Transfer-Encoding: 7bit 17, 20 -- X-Mailer: Microsoft Office Outlook 12.0 17, 20 -- Thread-Index: AdHsUtvT1I4RFoj8TK2PV1b0+6aOkQB2uqnwAAId8vA= 17, 20 -- Content-Language: en-gb ==============================End of - Headers==============================
Sorry to see your problems but maybe I can help. After 50 years with these instruments the problem you have I have seen very many times, I hope we can fix it for you? Set out below are the very basic principles for alignment of a TEM.
1. All lenses OFF, all apertures out and specimen out 2. High voltage on, filament heated to a position just short of the position that you would expect to be normal (de saturated source is bigger than a saturated source so often easier to find) 3. With gun alignment shift controls search for the beam. (if this is difficult use a raster motion of stepping one control in small steps and each step take the other control through its full range. 4. Once the beam is found adjust the gun tilt to obtain a maximum signal. 5. Switch on the first condenser lens, set the lens to a large spot size and adjust the gun tilt and shift for a maximum signal. 6. Switch on the second condenser lens and adjust Condenser 2 (often called brightness) to obtain the brightest spot and centre this with you illumination shift controls. 7. Switch on the objective lens and adjust Condenser 2 and the illumination shift controls again. 8. Set the magnification around 2,000X and switch on each of the other imaging lenses. Adjust Condenser 2 and the illumination shift controls to obtain the maximum intensity. 9. Switch to diffraction and obtain the diffraction point, shift this with the last lens in the column to the centre of the screen. This may be a mechanical or deflection coil alignment for image centre or equivalent name. 10. Insert a condenser aperture and with the Condenser 2 at focus and centred, expand the Condenser (clockwise) and adjust the aperture shadow to fit the screen with the aperture controls. Take the Condenser back to crossover and centre with the illumination shift if required, then repeat this section for a constant centre. Always expand the beam by turning the Condenser clockwise. 11. Adjust Condenser 2 to crossover and you will visualise the virtual source. Use the gun alignment tilt to set the "spot and halo" to be even on opposite sides, centre with the gun alignment shift;. Heat the filament until it is ALMOST a solid spot. (The folk law which says you must over saturate the filament slightly became out of date about 30 years ago!) 12. Insert a specimen and see that everything is operating near normal. As you focus you may find the image and the illumination alignment move, this is to be expected so correct the illumination with the illumination shift controls. Check section 10 again. 13. Switch on the objective alignment pulse system (I do not remember what it is called on an EM10), it will make the image pulse to help you find the objective axis centre. Use the illumination alignment tilt to place the centre of the pulse on the centre of the screen, correcting illumination movement with the illumination shift controls. 13. Switch to diffraction and reset the diffraction centre, insert an objective aperture.
I hope I have thought of everything, if not please come back to be.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in Message: Hi: Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj. aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj. x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards Login Host: 5.216.254.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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============================================
==============================Original Headers============================== 10, 53 -- From microscopy.listserver-at-gmail.com Mon Aug 1 18:47:13 2016 10, 53 -- Received: from mail-it0-f50.google.com (mail-it0-f50.google.com [209.85.214.50]) 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u71NlBmt016867 10, 53 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2016 18:47:11 -0500 10, 53 -- Received: by mail-it0-f50.google.com with SMTP id j124so187994007ith.1 10, 53 -- for {microscopy-at-microscopy.com} ; Mon, 01 Aug 2016 16:51:21 -0700 (PDT) 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; s=20120113; 10, 53 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 10, 53 -- :mime-version:in-reply-to:content-transfer-encoding; 10, 53 -- bh=lDPZ4k0Wks/EejPwoOBV6cJOMEAhZrhDBLthGI826P4=; 10, 53 -- b=dt0i3K1A/G1LuFcra8agEpwMAjSC9ZJypy5bxpPbAClSOQ8f6v+Zj88RX8Soh2OmdE 10, 53 -- zwUFEd0EP/RRQ+GyZYmjBhwMpp+Xop73H9067FTgrJ/y/wQ3Nhp5CJ8eihahr039sp+J 10, 53 -- J/R2ufnjW92+FG+GDRJD5DpdwObs9P9uGOYi+R07Lfd+Sh8Ad73D5X8HGCr59B6IWb2G 10, 53 -- /yTDXUaZ6qZYW+6yuTTHXPamzMNDuSgS67kDC/pAbJLtxm1Ez7HGzut0GG6oDlZAGr3j 10, 53 -- /DLRQVE7zG87MkwlehoXWn23LjtdYCnYEes489O3ZHMJ9xVyO3F9FRe3DcRsUwRzpTMH 10, 53 -- rXiQ== 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; s=20130820; 10, 53 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 10, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 10, 53 -- bh=lDPZ4k0Wks/EejPwoOBV6cJOMEAhZrhDBLthGI826P4=; 10, 53 -- b=SXYS6S+vjR3vZTinB4nJ8LDxtKGLmkpIbRpXSHtF4rHzQ9cTskwmR+no82h146M+Fs 10, 53 -- RNUgLycFFSjDKDgPdzF+Vqc07GIF/rzAe/Hmk3Y6kXQzezgCPH3eoCTjHWOviqWFlFmJ 10, 53 -- MnfqWJ5KJtAU0iBnHWv+yyiIfoMA2Ll8HS1V4WckWQwfbd8G5Cnsw8rNaADZ+FrSNRF+ 10, 53 -- SIVjP99j4Cy+bgAZcTC4B4ulke0zH4t5V+2HfFG3u1EoUribL2+9DheU2eD5cLnMWz6D 10, 53 -- J5jZiOtXR8noa/9qOduqzIEVepTbLg7f0g2rG1d8KMvmxB1eJrXjYx26Mm67+b2auhn+ 10, 53 -- rJhw== 10, 53 -- X-Gm-Message-State: AEkoouuamLqd9ty5KOU0vaRUbYFtyAlzar8Mcr4hg3Rw6a3eQ5fycIDo7PvkVwLIOuDWnA== 10, 53 -- X-Received: by 10.36.87.140 with SMTP id u134mr62579644ita.38.1470095480495; 10, 53 -- Mon, 01 Aug 2016 16:51:20 -0700 (PDT) 10, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([24.15.143.135]) 10, 53 -- by smtp.googlemail.com with ESMTPSA id i80sm118518ita.5.2016.08.01.16.51.19 10, 53 -- for {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 10, 53 -- Mon, 01 Aug 2016 16:51:19 -0700 (PDT) 10, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 10, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply 10, 53 -- {microscopylistserver-noreply-at-microscopy.com} 10, 53 -- Subject: viaWWW: Advice Needed Transmission electron microscope cm-10 10, 53 -- alignment, beam disappear in M magnification range with object aperture in 10, 53 -- References: {201608011529.u71FTD7o025749-at-ns.microscopy.com} 10, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- X-Forwarded-Message-Id: {201608011529.u71FTD7o025749-at-ns.microscopy.com} 10, 53 -- Message-ID: {cb5f9d32-811c-3454-af15-16c4ae5eeb5d-at-microscopy.com} 10, 53 -- Date: Mon, 1 Aug 2016 18:51:18 -0500 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 10, 53 -- Gecko/20100101 Thunderbird/45.2.0 10, 53 -- MIME-Version: 1.0 10, 53 -- In-Reply-To: {201608011529.u71FTD7o025749-at-ns.microscopy.com} 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 17, 20 -- From protrain-at-emcourses.com Thu Aug 4 04:54:07 2016 17, 20 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u749s693031930 17, 20 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2016 04:54:06 -0500 17, 20 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 17, 20 -- id {B57a311af0000} ; Thu, 04 Aug 2016 10:58:07 +0100 17, 20 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Thu, 4 Aug 2016 10:58:06 +0100 17, 20 -- From: "Steve Chapman" {protrain-at-emcourses.com} 17, 20 -- To: {researchers4u-at-gmail.com} 17, 20 -- Cc: {microscopy-at-microscopy.com} 17, 20 -- Subject: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10 17, 20 -- Date: Thu, 4 Aug 2016 10:58:17 +0100 17, 20 -- Message-ID: {007001d1ee36$b9859880$2c90c980$-at-com} 17, 20 -- MIME-Version: 1.0 17, 20 -- Content-Type: text/plain; 17, 20 -- charset="us-ascii" 17, 20 -- Content-Transfer-Encoding: 7bit 17, 20 -- X-Mailer: Microsoft Office Outlook 12.0 17, 20 -- Thread-Index: AdHsUtvT1I4RFoj8TK2PV1b0+6aOkQB2uqnwAAId8vAAABjbwA== 17, 20 -- Content-Language: en-gb ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpshield-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] October Biological TEM Workshop
Message: This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Monday, October 24 through Wednesday, October 26, 2016, 8am-5pm each day
Where: Georgia Electron Microscopy Labs, 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. gem.uga.edu
Deadline: October 15, 2016
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Anyone know who is service or providing parts for Bal-Tec equipment? Both my CPD and Med -20 evaporator are shot and require some TLC to remain operational.
Thanks,
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY
==============================Original Headers============================== 9, 26 -- From WHITTAKS-at-si.edu Thu Aug 4 11:08:46 2016 9, 26 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u74G8cl0006585 9, 26 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2016 11:08:40 -0500 9, 26 -- Received: from SI-MSECHT03.US.SINET.SI.EDU (si-msecht03.us.sinet.si.edu [160.111.49.155]) 9, 26 -- by si-mailout03.si.edu (Postfix) with ESMTP id 3B3DD6CE0E4 9, 26 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2016 12:12:56 -0400 (EDT) 9, 26 -- Received: from SI-MSEDAG04.US.SINET.SI.EDU ([169.254.4.84]) by 9, 26 -- SI-MSECHT03.US.SINET.SI.EDU ([160.111.49.149]) with mapi id 14.03.0279.002; 9, 26 -- Thu, 4 Aug 2016 12:12:55 -0400 9, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 9, 26 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 26 -- Subject: Bal-Tec/Balzers coater and cpd service 9, 26 -- Thread-Topic: Bal-Tec/Balzers coater and cpd service 9, 26 -- Thread-Index: AdHuarv9mIFWSGa8QAmN5Tj+JZ9J3w== 9, 26 -- Date: Thu, 4 Aug 2016 16:12:55 +0000 9, 26 -- Message-ID: {5D4F637E5C599649A11693E3B6EAC6293FB9AD9F-at-si-msedag04.US.SINET.SI.EDU} 9, 26 -- Accept-Language: en-US 9, 26 -- Content-Language: en-US 9, 26 -- X-MS-Has-Attach: 9, 26 -- X-MS-TNEF-Correlator: 9, 26 -- x-originating-ip: [160.111.49.170] 9, 26 -- Content-Type: text/plain; charset="us-ascii" 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Transfer-Encoding: 8bit 9, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u74G8cl0006585 ==============================End of - Headers==============================
I would like to hear privately from anyone who has a Pelco easiGlow Dual Chamber Glow Discharge system or has a EMS GLOQUBE-D Dual Camber Glow Discharge System. I would like to know your experiences with either one of these systems.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 6, 36 -- From tbargar-at-unmc.edu Mon Aug 8 09:18:24 2016 6, 36 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u78EINco010257 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Aug 2016 09:18:23 -0500 6, 36 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 6, 36 -- by Outbound.unmc.edu (Proprietary) with SMTP id 1C6492A1620 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Aug 2016 08:49:43 -0500 (CDT) 6, 36 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 6, 36 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 6, 36 -- (No client certificate requested) 6, 36 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id 53E0D2A161A 6, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Aug 2016 08:49:42 -0500 (CDT) 6, 36 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 6, 36 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 6, 36 -- 14.03.0301.000; Mon, 8 Aug 2016 09:18:14 -0500 6, 36 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Subject: Glow discharge systems 6, 36 -- Thread-Topic: Glow discharge systems 6, 36 -- Thread-Index: AdHxf0kYfkUnjrMiQF2cbNAJuQ6kaQ== 6, 36 -- Date: Mon, 8 Aug 2016 14:18:13 +0000 6, 36 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE87A780-at-UNMCEX2.unmcresforest.org} 6, 36 -- Accept-Language: en-US 6, 36 -- Content-Language: en-US 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-originating-ip: [10.8.64.15] 6, 36 -- Content-Type: text/plain; charset="us-ascii" 6, 36 -- MIME-Version: 1.0 6, 36 -- X-VPM-MSG-ID: 001e2d40-e00d-45a2-93da-f2e7983d666a 6, 36 -- X-VPM-HOST: zixvpm02.unmc.edu 6, 36 -- X-VPM-GROUP-ID: f9066000-40d6-4844-a1c1-842b7b876e98 6, 36 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 6, 36 -- X-VPM-IS-HYBRID: 0 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u78EINco010257 ==============================End of - Headers==============================
Our lab is trying to optimize the reaction conditions for the photooxidation of DAB using the MiniSOG construct, but we are encountering difficulties getting a good electron-dense reaction product to form. Does anyone have first-hand experience working with the MiniSOG construct, or even analyzing samples for others who have done this reaction?
Currently, we are transfecting HEK 293-T cells or HeLa cells with 200ng of plasmid using the FuGENE transfection reagent on Thermanox coverslips in 6-well cell culture dishes. We have been examining the fluorescent signal before fixation, and the plasmid that gives us the most promising signal is a peroxisome-localized MiniSOG/mCherry fusion. Therefore, we are focusing on optimizing peroxisome labeling. We are doing a 30 minute fix in 2% glutaraldehyde in 0.1M cacodylate buffer, 3 x 5min buffer washes, then a 1 hour blocking in 50mM glycine, 10mM KCN, 5mM aminotriazole (buffered) followed by 3 x 5 min buffer washes. The light exposure is being performed on a Leica wide-field inverted microscope using the EL6000 light source, which has a 120W metal halide bulb from what I can find online. We are going through the 20x long working distance objective (I don't have the NA handy), and exposure is 2-5 minutes/FOV. Oxygen is being supplied by a portable tank and is both bubbled into the DAB solution beforehand and passed over the well during exposure.
Right now the fluorescent signal looks good, but when we are finished processing and embedding, there is little to no electron-dense product in the thin sections. Any suggestions are welcome.
Thank you, Nick
Nicholas Conoan Electron Microscopy Specialist University of Nebraska Medical Center Wittson Hall 2013 402-559-7347
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==============================Original Headers============================== 10, 36 -- From nicholas.conoan-at-unmc.edu Mon Aug 8 11:39:55 2016 10, 36 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u78GdthA004348 10, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Aug 2016 11:39:55 -0500 10, 36 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 10, 36 -- by Outbound.unmc.edu (Proprietary) with SMTP id D8AD824A6ED 10, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Aug 2016 11:38:32 -0500 (CDT) 10, 36 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 10, 36 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 10, 36 -- (No client certificate requested) 10, 36 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 1355224A3A2 10, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Aug 2016 11:38:32 -0500 (CDT) 10, 36 -- Received: from UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93]) by 10, 36 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 10, 36 -- 14.03.0301.000; Mon, 8 Aug 2016 11:39:46 -0500 10, 36 -- From: "Conoan, Nicholas H" {nicholas.conoan-at-unmc.edu} 10, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 36 -- Subject: Need advice to optimize MiniSOG assay 10, 36 -- Thread-Topic: Need advice to optimize MiniSOG assay 10, 36 -- Thread-Index: AdHxjuDbkkwGUJIuQlWiEl8+sVBd6w== 10, 36 -- Date: Mon, 8 Aug 2016 16:39:46 +0000 10, 36 -- Message-ID: {819E78F6D4C7264C973436613769DED18E0089F2-at-UNMCEX3.unmcresforest.org} 10, 36 -- Accept-Language: en-US 10, 36 -- Content-Language: en-US 10, 36 -- X-MS-Has-Attach: 10, 36 -- X-MS-TNEF-Correlator: 10, 36 -- x-originating-ip: [10.8.64.14] 10, 36 -- Content-Type: text/plain; charset="us-ascii" 10, 36 -- MIME-Version: 1.0 10, 36 -- X-VPM-MSG-ID: df5d2b02-d197-41e9-8926-83af00b85cf7 10, 36 -- X-VPM-HOST: zixvpm01.unmc.edu 10, 36 -- X-VPM-GROUP-ID: 59ab7aa8-784d-4386-85cc-47beaad3e242 10, 36 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 10, 36 -- X-VPM-IS-HYBRID: 0 10, 36 -- Content-Transfer-Encoding: 8bit 10, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u78GdthA004348 ==============================End of - Headers==============================
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Title-Subject: [Filtered] EELS Training in October 2016
Message: EELS & EFTEM Analysis Training School October 2016 October 11-14, 2016 Gatan R&D Headquarters Pleasanton, CA USA
Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional and chemical information from sub-nanometer areas in the sample. As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
-Fundamentals of EELS and energy-filtered imaging in TEM -Principles of operation of EFTEM and EELS systems -Optimization of EFTEM and EELS data acquisition -Quantification of elemental composition -Other information provided by EFTEM/EELS and how best to extract it -Use of EELS signals to form maps of elemental and chemical composition -EFTEM and STEM EELS spectrum imaging techniques -Identification of material phases via EELS fine structure mapping -Applications to biological and physical science specimens
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Email: yankova-at-uchc.edu Name: Maya Yankova
Organization: University of Connecticut Health Center
Title-Subject: [Filtered] Immunogold Negative staining of Exosomes
Message: Dear All, We have a researcher who is interested in immunogold labeleing and negative staining of exosomes isolated from Mouse macrophages.So far we have done immunogold and negative staining with CD63 rabbit from SBI and didn't get any labeling. I would like to ask you, if you can recommend other companies we can get exosomal markers : CD63,CD9 etc. and do immunogold negative staining on whole mount exosomes. Thank you in advance for your help!
Maya Yankova, Research Associate Central Electron Microscopy Facility Department of Molecular, Microbial & Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3305 ph: 860.679.2395 yankova-at-neuron.uchc.edu
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Email: chiyen-at-uw.edu Name: Chiyen Miller
Organization: University of Washington
Title-Subject: [Filtered] Job opening: TEM electron microscopy
Message: University of Washington Medical Center Anatomic Pathology has a job opening for TEM electron microscopy. For inquiries please go to the University of Washington employment opportunities website and under find a job, search the req #124675. Thank you!
Chiyen Miller Lead, EM Lab UWMC
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Title-Subject: [Filtered] Correlative Light and Electron Microscopy
Message: Hi everyone, Have you ever tried the CLEM software of TESCAN, named AURORA?
I would like to hear your comments about this system, functionality & benefits.
Thanks a Lot.
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Email: john.wailes-at-zeiss.com Name: John Wailes
Organization: Carl Zeiss
Title-Subject: [Filtered] EM/XRM Specialist position with Carl Zeiss
Message: Carl Zeiss Microscopy is looking for a Life Sciences, EM/XRM systems specialist, to cover a territory in the eastern half of the United States. If you are interested, please contact John Wailes using the details below.
John Wailes Carl Zeiss Microscopy, LLC Systems Sales Manager, Academia EM/XRM Mobile: (845) 553-5339 john.wailes-at-zeiss.com
YOUR JOB
* Selling and support of ZEISS instrumentation and related EM/XRM components.
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OUR REQUIREMENTS
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With just under 25,000 employees, ZEISS is one of the global leaders in the optical and optoelectronic industries and has been contributing to technological progress for over 165 years. ZEISS develops and distributes lithography optics, measuring technology, microscopes, medical technology, eyeglass lenses, camera and cine lenses, binoculars and planetarium technology.
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Email: see.wee.chee-at-gmail.com Name: See Wee
Title-Subject: [Filtered] Lego Ideas: SEM, TEM, XRD
Message: Let's all vote for this guy and make these into real Lego products. :)
https://ideas.lego.com/projects/102281
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Email: marie.cantino-at-uconn.edu Name: Marie Cantino
Organization: Dr.
Title-Subject: [Filtered] section folds
Message: Does anyone know how to prevent section folds in 1 or 2 micron thick sections of plant stem material embedded in Spurrs resin? The sections are fairly large, about 4 x 7 mm, cut on a histo diamond, then placed on water drops on Fisher Superfrost Plus slides and dried on a slide warmer at about 70 degrees C. We get multiple section folds parallel to the short axis of the rectangle, that start at the resin surrounding the tissue and project into the tissue at the center of the block. Thanks! -Marie
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Well, they don't have the correct graphics on some of the controls, but years ago based on a challenge to my son, he made me these out of his own Lego stash:
http://www.mta.ca/dmf/lego.html
Somewhat sadly, many visitors to the lab find these more interesting than the actual instruments! Sigh.
Jim
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--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
I have used DTSA for modeling S/TEM spectra using the MC software. I'm not sure I would use the software for high precision work, but it should be OK for things like detection limits, etc.
There are 2 main differences between TEM and SEM modeling: beam voltage and the thin film sample. DTSA allows for a thin film sample where you can set the substrate to nothing. The kV should affect only the ionization cross-section. Once the overvoltage gets above 4-5, there is a relatively slow variation in the cross-section. (see for example, Williams & Carter 2d ed fig 4.4). Raynald Gauvin's work (M&M 2012 #1000) suggests that there should be little difference in the models at the higher kVs. The agreement of the models gives me a bit more confidence that the cross-sections are meaningful.
You can always collect spectra from known samples and compare them to the MC models and see how close they come.
If anyone has more detailed info about the modeling validity, I'd like to know too!
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} , yankova-at-uchc.edu {yankova-at-uchc.edu}
Hi Maya The gold labeling will only work if the epitope is on the external side of the exosome membrane. Remember, they are like tiny balloons and only the external surface is exposed to the antibody. Not a solution, just something to consider. Lee
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Email: yankova-at-uchc.edu Name: Maya Yankova
Organization: University of Connecticut Health Center
Title-Subject: [Filtered] Immunogold Negative staining of Exosomes
Message: Dear All, We have a researcher who is interested in immunogold labeleing and negative staining of exosomes isolated from Mouse macrophages.So far we have done immunogold and negative staining with CD63 rabbit from SBI and didn't get any labeling. I would like to ask you, if you can recommend other companies we can get exosomal markers : CD63,CD9 etc. and do immunogold negative staining on whole mount exosomes. Thank you in advance for your help!
Maya Yankova, Research Associate Central Electron Microscopy Facility Department of Molecular, Microbial & Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3305 ph: 860.679.2395 yankova-at-neuron.uchc.edu
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X-from: Cantino, Marie {marie.cantino-at-uconn.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
We have tried this technique with dichloroethylene without success. I think the sections are really too big, but we have gotten some other suggestions that have reduced (but not eliminated) the folds. Thanks for your suggestion!
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} On Aug 8, 2016, at 10:43 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } Subject: RE: [Microscopy] viaWWW:how to prevent section folds } Date: Wed, 3 Aug 2016 11:58:38 +0000 } X-from: Joachim Siegmund {jsiegmund-at-7thwavelabs.com} } To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} } } } } Did you try Ether on a Q-Tip, hovering over the section on the water drop? } } Regards, } } Joachim Siegmund } Image Analyst } Seventh Wave Laboratories LLC } 19 Worthington Access Drive } Maryland Heights, MO 63043 } jsiegmund-at-7thwavelabs.com } www.7thwavelabs.com } } } -----Original Message----- } X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] } Sent: Tuesday, August 02, 2016 10:49 PM } To: Joachim Siegmund } Subject: [Microscopy] viaWWW:how to prevent section folds } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: marie.cantino-at-uconn.edu } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } marie.cantino-at-uconn.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: marie.cantino-at-uconn.edu Name: Marie Cantino } } Organization: Dr. } } Title-Subject: [Filtered] section folds } } Message: Does anyone know how to prevent section folds in 1 or 2 micron thick sections of plant stem material embedded in Spurrs resin? The sections are fairly large, about 4 x 7 mm, cut on a histo diamond, then placed on water drops on Fisher Superfrost Plus slides and dried on a slide warmer at about 70 degrees C. We get multiple section folds parallel to the short axis of the rectangle, that start at the resin surrounding the tissue and project into the tissue at the center of the block. } Thanks! -Marie } } Login Host: 137.99.47.142 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. 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Joseph, You can also use a 1:1 mixture of the Epon and Spurr's resin. Use only half the concentration of each catalyst (0.75% of DMP-30 and DMAE). Use extended parts of your transition solvent to resin (30-50-90% in resin-2-3 hrs./overnight rocking ), then when you get to pure changes use a 15 psi vacuum for at least one of these pure resin changes. I then add an extra day of pure resin changes. I agree with Lou Ann that you must mix and centrifuge the pellet for each change. I have found that some bacteria will Infiltrate well while others (KO) may not. Good luck.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, August 08, 2016 10:53 PM To: mdelann1-at-jhmi.edu
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Although the remark is generally true, when it comes to CD it only makes sense if it labels external epitopes. For this very reason I would suggest to do pre-embeddings labeling. Also you may consider a positive control to control if you are able to label the marker when you are sure that one is present.
regards Stephane -------------------------------------------- On Wed, 8/10/16, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
Subject: [Microscopy] viaWWW: Immunogold Negative staining of Exosomes To: nizets2-at-yahoo.com Date: Wednesday, August 10, 2016, 2:18 PM
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X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} , yankova-at-uchc.edu {yankova-at-uchc.edu}
Hi Maya The gold labeling will only work if the epitope is on the external side of the exosome membrane. Remember, they are like tiny balloons and only the external surface is exposed to the antibody. Not a solution, just something to consider. Lee
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Email: yankova-at-uchc.edu Name: Maya Yankova
Organization: University of Connecticut Health Center
Title-Subject: [Filtered] Immunogold Negative staining of Exosomes
Message: Dear All, We have a researcher who is interested in immunogold labeleing and negative staining of exosomes isolated from Mouse macrophages.So far we have done immunogold and negative staining with CD63 rabbit from SBI and didn't get any labeling. I would like to ask you, if you can recommend other companies we can get exosomal markers : CD63,CD9 etc. and do immunogold negative staining on whole mount exosomes. Thank you in advance for your help!
Maya Yankova, Research Associate Central Electron Microscopy Facility Department of Molecular, Microbial & Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3305 ph: 860.679.2395 yankova-at-neuron.uchc.edu
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Does anyone have a protocol for processing Cells grown in hydrogel for TEM, that will not shrink the hydrogel?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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Email: Marie.cantino-at-uconn.edu Name: Marie Cantino
Organization: University of Connecticut, Biosciences Electron Microscopy Laboratory
Title-Subject: [Filtered] how to prevent section folds
Message: Thanks to the MANY people who took time to respond to my question about section folds. Unfortunately the person who is actually testing these tips is on vacation, so I can't comment yet on which ones worked best for us, but since several people requested a summary of responses, I have organized them below. FIXATION AND EMBEDDING 1. Increase fixation time. If folds are at the centers of the sections there may be a change in tissue fixation (fixed at the periphery but not at center). 2. Use long infiltration times, especially if cell walls are thick. For resin-solvent steps and first 100% step, use Spurrs WITHOUT catalyst (overnight) followed by two changes of 100% Spurrs WITH catalyst for a full day. 3. Try matching the resin hardness to the tissue hardness by using a different resin mixture.
TRIMMING 4. Make razor blade cuts in the embedding media around the sample then mount sections in the embedding medium to make the cuts disappear (not clear whether the respondent had tried this) 5. Make the block face smaller!! [the block face seemed very large to me, but Im an electron microscopist so anything larger than 1 mm seems large. However, it was helpful to get conformation that this is large even for semithin sections]. 6. Trim a trapezoid, not a rectangle. 7. Trim the tissue at the bottom edge or on all sides to eliminate empty resin
MICROTOMY 8. Try cutting sections thinner, 0.4-0.5 microns, and stain with 1% toluidine blue stain. 9. Try cutting sections thicker. 10. Apply solvent vapors to sections still in the knife boat by waving a Q-tip or sliver of filter paper soaked with solvent over the sections. Solvents recommended were chloroform or ether (we have also used trichloroethylene). Note toxicity of these solvents by inhalation! 11. Use a heat pen to achieve the same effect as above. 12. Wet a plastic bottle cap with chloroform and hold the cap over the boat as the vapors evaporated. Chloroform vapor is heavier than air, and creates a downward force on the surface of water in your boat. PICK-UP 13. Pick sections up and float them on a container of hot water to stretch them before transferring them to the slide to dry them. 14. Pick sections up with a loop (e.g., Perfect Loop) and immerse them in the water droplet rather than trying to roll the loop over to get the sections onto the slide. Come straight down on top of the water droplet with the loop (section side up) and let the sections float off of the loop and onto the water surface. 15. Add ethylene glycol (maybe 1%?) or acetone ( { 10%?) to the water drop on the slide (not in the trough). However, be aware that section dimensions may change. One respondent also suggested adding ethanol at this step, but had not tried this with plastics. 16. Switch to untreated glass slides. Superfrost Plus slides have a surface treatment that will make sections adhere more readily, not allowing them to spread out as they dry down and causing section puckering.
DRYING 17. Put a small open glass Petrie dish on the hot plate. Heat it. Put the slide, drip side down over the dish to evaporate. If necessary, flip dry slide over and heat to a higher temp to promote adhesion. 18. Apply solvent vapors (chloroform, xylene) while drying the sections on the slide warmer. Several suggestions of how to do this were to place a drop of solvent in the lid of a petri dish over the sections while drying or to hover a chloroform soaked Q-tip over slide during drying 19. Lower the temperature on the slide warmer so that sections will dry VERY slowly, then reheat after all water is gone to improve adhesion 20. Raise the temperature on the slide warmer slightly then gently agitate the slide while it is on the hotplate to evenly dry the water. 21. Place a few drops of acetone on a Q-tip on the frosted end of your slide, cover your slide with a Petri dish and allow the water to dry down on your hotplate. The increased pressure in the dome produced by the Petri dish from the evaporated acetone helps your sections to dry flat. Have the hotplate set hot enough to boil water. 22. Heat a small open glass Petri dish on the hot plate. Put the slide, drip side down over the Petri dish during drying. 23. Look at https://www.researchgate.net/publication/19412292_Flat_Adherent_Well-Contrasted_Semithin_Plastic_Sections_for_Light_Microscopy
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Email: Desertrat99-at-verizon.net Name: Eric Rosen
Organization: Ucla
Title-Subject: [Filtered] Training an EM tech from zero experience. Message: Howdy all, I was curious to know how difficult is it to train someone to be an TEM tech when starting at zero experience? Thanks
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==============================Original Headers============================== 13, 52 -- From microscopy.listserver-at-gmail.com Tue Aug 16 07:33:46 2016 13, 52 -- Received: from mail-it0-f44.google.com (mail-it0-f44.google.com [209.85.214.44]) 13, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u7GCXkRA014424 13, 52 -- for {microscopy-at-microscopy.com} ; Tue, 16 Aug 2016 07:33:46 -0500 13, 52 -- Received: by mail-it0-f44.google.com with SMTP id e63so32059848ith.1 13, 52 -- for {microscopy-at-microscopy.com} ; Tue, 16 Aug 2016 05:34:07 -0700 (PDT) 13, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 52 -- d=gmail.com; s=20120113; 13, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 13, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 13, 52 -- bh=mzP3LQGt1EtDrC54LEpVosapWbuaaSPlYm2CU0Chm0c=; 13, 52 -- b=p1eMuMA06mIMA+J5TXY+2vEJrOV48JeW2gmueXOdh4PHu6B9BKcWBy2jE2ZAy2sE8N 13, 52 -- +nRqfdKGA/9OyTpkXpn5SwvcJexl+18zOP5S4pFX/IFM9yhRZiionJVEpHux5ineDMOf 13, 52 -- C67IbS0visT6xLKOA1VXEv4CWpo8V24o1ZYpu3WCDXNwOKaRjq1b2TsH4lMijued94M9 13, 52 -- WgrbX6JTXSqTE2HdGYxqIBIdDYkQ9qNViBxBQvQNyyn6vgdsOG7eEDnPnS4iR3AQ3oa9 13, 52 -- aysRjZJGBRfXTLV5PcBrCGEZnugjRNH1pGhn131Al/MDIOuWSM4ccGyjRQspj6Jw/3uK 13, 52 -- c9gA== 13, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 52 -- d=1e100.net; s=20130820; 13, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 13, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 13, 52 -- bh=mzP3LQGt1EtDrC54LEpVosapWbuaaSPlYm2CU0Chm0c=; 13, 52 -- b=O51/FLR6QIQF+yvEhKAJAUk6XJhnzl+Ccxd9zn1Zrg6mQT00GWu6IOPJ3NUTPhxKOz 13, 52 -- bbDeRVDz1ISmbTZa+z0w163wurogZjVUPcQZgumdO6dMmtmSNbo1XlZer/0ihO0kG+AN 13, 52 -- XFLRfgfEl+xjPsXaVV06EKRwHlyafF9Lyv7oWh1jU4soRsKuYhA/HjLdc1jFmVo2IBK4 13, 52 -- 80BL9ZOkLhNIzIJfqhnrVuhAsJ2Dm43Wdorz30/0CR1YNJl9sURtTUg3sYsRYtjjAuIe 13, 52 -- r/SpDOtDME8x3z54b6wiSBOQC3s2UfOH+TaA4nSl+OBKY8BNiyNkkJvbmvLVPzCf9FRS 13, 52 -- qAfA== 13, 52 -- X-Gm-Message-State: AEkooutM9hz5OuY8Mj+OU6++ua9mtDHpQk8NpKNdW1KKpoQS1luEAgrGCJJppfwpfeGH2A== 13, 52 -- X-Received: by 10.36.16.138 with SMTP id 132mr22079684ity.60.1471350846952; 13, 52 -- Tue, 16 Aug 2016 05:34:06 -0700 (PDT) 13, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([24.15.143.135]) 13, 52 -- by smtp.googlemail.com with ESMTPSA id x72sm6874061ite.0.2016.08.16.05.34.06 13, 52 -- for {microscopy-at-microscopy.com} 13, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 52 -- Tue, 16 Aug 2016 05:34:06 -0700 (PDT) 13, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 52 -- {microscopylistserver-noreply-at-microscopy.com} 13, 52 -- Subject: viaWWW: Training an EM tech from zero experience 13, 52 -- References: {201608160217.u7G2H8xb021174-at-ns.microscopy.com} 13, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 52 -- X-Forwarded-Message-Id: {201608160217.u7G2H8xb021174-at-ns.microscopy.com} 13, 52 -- Message-ID: {c9849316-42e9-db8c-69ad-591fb4f3f2b3-at-microscopy.com} 13, 52 -- Date: Tue, 16 Aug 2016 07:34:05 -0500 13, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 13, 52 -- Gecko/20100101 Thunderbird/45.2.0 13, 52 -- MIME-Version: 1.0 13, 52 -- In-Reply-To: {201608160217.u7G2H8xb021174-at-ns.microscopy.com} 13, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: kbustillo-at-lbl.gov Name: Karen Bustillo
Organization: NCEM, Molecular Foundry, LBNL
Title-Subject: [Filtered] TEM Open Position Technical Staff NCEM, Berkeley, CA
Message: The National Center for Electron Microscopy (NCEM), at the Molecular Foundry, Lawrence Berkeley National Lab is seeking candidates to fill an open position as a member of the technical staff. The Molecular Foundry is a DOE funded User Facility.
The technical staff position is in the area of user support for TEM sample preparation and in situ TEM operation. We are looking for an outstanding candidate to support and contribute to the user and science programs at the Molecular Foundry. We are specifically encouraging all qualified women and candidates from underrepresented groups to apply. The link to the job posting is below:
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Email: mattinson-at-Geology.cwu.EDU Name: Chris Mattinson
Organization: Central Washington University
Title-Subject: [Filtered] SEM instrument tech job, CWU
Message: Hi All,
We're currently searching for an instrument tech whose primary charge will be our new SEM, and would appreciate your help to forward this ad to qualified potential applicants. Screening begins Sept. 1.
Chris
Engineering Technician 3 - Geological Sciences Department at Central Washington University (CWU)
The Engineering Technician 3 oversees the Geological Sciences Departments major instrumentation and associated laboratory facilities in the new science building at Central Washington University. This oversight includes: design and modification of systems and peripherals to achieve desired geological applications; maintenance, operation and repair of instruments; training users; and overseeing safety protocol. This position oversees the operation and maintenance of a FEI Quanta 250FEG scanning electron microscope (SEM) with users from multiple departments at CWU. The position will also oversee other instruments in the Geological Sciences instrumentation laboratory. For more information and to apply, see https://careers.cwu.edu (Job ID 729). CWU is an AA/EEO/Title IX/Veteran/Disability Employer.
__ Chris Mattinson Associate Professor & Graduate Program Coordinator Department of Geological Sciences Central Washington University 400 E. University Way, Ellensburg, WA 98926-7418 Tel: 509.963.1628
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Email: the88gtu-at-yahoo.com Name: Joel Leyva
Organization: SJDC Graduate
Title-Subject: [Filtered] TEM fluorescent screen metal plate stuck at 90 degrees
Message: Hello fellow microscopists, I have a problem with a JEOL JEM-CX II. I replaced the binoculars and attempted to view the sample on the fluorescent screen but when the plate was moved to the 45 degree it wouldn't go back down. I then pushed the 90 (vertical) to see if it will go back that way but it got stuck. I could hear a motor running for 20 seconds before it died off. I can personally send images so you can see what is meant by fluorescent plate. Thank you.
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Email: johanna.hoog-at-gu.se Name: Johanna Hoog
Organization: University of Gothenburg
Title-Subject: [Filtered] Wanted: microtome
Message: Dear all, I am starting a EM lab from scratch here in Gothenburg and would like to ask you if anyone has a used microtome that functions but is no longer used in your labs? I would pay for transport and if it is a newer version I could also pay something for the machine itself. Please contact me with info. Many thanks in advance!
Johanna Hoog Assistant professor Department of Chemistry and Molecular Biology University of Gothenburg
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Exciting opportunity for an experienced electron microscopist to join MRC-LMB Cambridge, UK.
This position provides a rare opportunity for an experienced researcher or engineer to combine laboratory/technical management with research or technical development. You will undertake independent scientific or technical research in the area of cryo-electron tomography and subtomogram averaging, and if appropriate, FIB milling. You will also implement methods and instrumentation for cryo-electron tomography, helping to maintain, develop and support cryo-electron tomography capabilities at the LMB.
The EM facility at LMB currently includes two Titan Krios microscopes, Polara, Scios dual-beam, two 200kV FEG microscopes and 3 T12/Spirit microscopes. In addition, the EM facility has a suite of associated equipment for sample preparation. You will be a member of the lab of John Briggs, which is currently based at the EMBL in Heidelberg, but will be based at the LMB from Jan 2017. Information about the Briggs group is available here: http://www.embl.de/research/units/scb/briggs/index.html
More information about the job is available here: mrc.io/2bzQOR1 The closing date for applications is 18th September.
Feel free to get in touch with John Briggs if you have questions. john.briggs-at-embl.de
==============================Original Headers============================== 5, 39 -- From wim.hagen-at-me.com Fri Aug 26 06:18:03 2016 5, 39 -- Received: from pv33p04im-asmtp002.me.com (pv33p04im-asmtp002.me.com [17.143.181.11]) 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u7QBI3xr017663 5, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Aug 2016 06:18:03 -0500 5, 39 -- Received: from process-dkim-sign-daemon.pv33p04im-asmtp002.me.com by 5, 39 -- pv33p04im-asmtp002.me.com 5, 39 -- (Oracle Communications Messaging Server 7.0.5.38.0 64bit (built Feb 26 2016)) 5, 39 -- id {0OCI00N00KNOD100-at-pv33p04im-asmtp002.me.com} for Microscopy-at-microscopy.com; 5, 39 -- Fri, 26 Aug 2016 11:18:48 +0000 (GMT) 5, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=me.com; s=4d515a; 5, 39 -- t=1472210328; bh=5b8dEJQcWTRoUgUoWCT5vYCXoAKVnzJDANdFwXcyLoQ=; 5, 39 -- h=From:Content-type:Subject:Message-id:Date:To:MIME-version; 5, 39 -- b=pWvns3yiVe06b/+PoLjd+5kdCDAYzLCEEhYSzv2R6a0GSiqnifA7GJctZo+yId2mu 5, 39 -- wqu4xpABBloWNe1HP6BaALLHtdF6V0Rzn/FbABbCSdEmLBZxx7JLKnWxxGqU37ljjR 5, 39 -- 2TnvGqTKAxpyxYXXdh/J4YKJ/zVyVXrtAf220PnEC+7JpBZ/kfpzAlEv5cp4aN3Sh2 5, 39 -- 2yQ9WniGxoNg0AJftyVVYbnmwyoVVjjO4VOgxVRzd4s+x/oXXDMQV/9vEOA5ijLq/5 5, 39 -- 3MTiyxQFznOq9eI57RcZbW0k/Uqbf0QjfqZKXr8dDGi9ycok2eyzt6xUxTh1v+ykMQ 5, 39 -- rG9dFxKJm1XIw== 5, 39 -- Received: from wim-hagens-mbp.wlan.embl.de (unknown [194.94.44.220]) 5, 39 -- by pv33p04im-asmtp002.me.com 5, 39 -- (Oracle Communications Messaging Server 7.0.5.38.0 64bit (built Feb 26 2016)) 5, 39 -- with ESMTPSA id {0OCI00KGIKR9A600-at-pv33p04im-asmtp002.me.com} for 5, 39 -- Microscopy-at-microscopy.com; Fri, 26 Aug 2016 11:18:48 +0000 (GMT) 5, 39 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, 5, 39 -- definitions=2016-08-26_07:,, signatures=0 5, 39 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 5, 39 -- clxscore=1015 suspectscore=1 malwarescore=0 phishscore=0 adultscore=0 5, 39 -- bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 5, 39 -- engine=8.0.1-1603290000 definitions=main-1608260134 5, 39 -- From: Wim Hagen {wim.hagen-at-me.com} 5, 39 -- Content-type: text/plain; charset=us-ascii 5, 39 -- Subject: position for experienced electron microscopist at MRC-LMB 5, 39 -- Message-id: {2C4263E6-8C92-4C4D-8D57-0BF71B82406F-at-me.com} 5, 39 -- Date: Fri, 26 Aug 2016 13:18:45 +0200 5, 39 -- To: Microscopy-at-microscopy.com 5, 39 -- MIME-version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 5, 39 -- X-Mailer: Apple Mail (2.3124) 5, 39 -- Content-Transfer-Encoding: 8bit 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u7QBI3xr017663 ==============================End of - Headers==============================
We currently have a permanent position available for a structure-oriented early career scientist at AgResearch Lincoln in the South Island of New Zealand.
Full details here https://careers.sciencenewzealand.org/jobdetails/ajid/r7WV7/Fibre-Structural-Biology-Scientist,18510.html
Our research is often uses a variety of techniques, and the substrates involve both soft tissue (e.g., skin and hair follicles) as well as keratin fibres (which are effectively a complex nano-composite biomaterial). While we don't expect applicants to be experts on keratin fibres or follicle biology, we are looking for someone who can fit into this role easily and brings good experimental design capability and penchant for technical/instrumentation/analysis innovation.
Queries should be directed via the web page above.
Kind regards Duane
____________________________ Dr Duane P Harland Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz AgResearch Limited Lincoln Research Centre Cnr Springs Rd & Gerald Street, Lincoln Private Bag 4749 Christchurch 8140, New Zealand T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz Farming Food and Health. First Te Ahuwhenua Te Kai me te Whai Ora. Tuatahi
======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. =======================================================================
==============================Original Headers============================== 9, 38 -- From Duane.Harland-at-agresearch.co.nz Fri Aug 26 18:54:29 2016 9, 38 -- Received: from out1102.nz.smxemail.com (out1102.nz.smxemail.com [203.84.134.33]) 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u7QNsS2h007296 9, 38 -- for {microscopy-at-microscopy.com} ; Fri, 26 Aug 2016 18:54:29 -0500 9, 38 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 9, 38 -- q=dns/txt; i=-at-nz.smxemail.com; t=1472255727; 9, 38 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 9, 38 -- bh=r9v61weuEYoiF0bANnmoTBKYhMr/2T7OVTbXiSx/UJg=; 9, 38 -- b=zZiI9SXwng2A6AJT1jSJWDY/yI32FZsvAcI6YvXwtsDY7Tg3ke5maeeKpg5szv5O 9, 38 -- N3hBNydNAq0WqmSPFNJ5SrXzy9BK0mjewFDFzBRCM2F4sKkIc4Gp9TiBYpHmDYht 9, 38 -- hXHn2N5xRLVHdVAc/w8IH7pJ/zTnAfWgaTrTQc0aTuM=; 9, 38 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 9, 38 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 9, 38 -- with cipher AES128-SHA (128/128 bits)) 9, 38 -- id 57C0D6EF-32E40B90-at-mta1106.omr; 9, 38 -- Fri, 26 Aug 2016 23:55:27 +0000 9, 38 -- Received: from RUAEXCPV02.agresearch.co.nz (147.158.67.24) by 9, 38 -- invexcpv03.agresearch.co.nz (147.158.130.2) with Microsoft SMTP Server (TLS) 9, 38 -- id 14.3.294.0; Sat, 27 Aug 2016 11:55:24 +1200 9, 38 -- Received: from RUAEXCPV01.agresearch.co.nz ([169.254.2.201]) by 9, 38 -- ruaexcpv02.agresearch.co.nz ([147.158.67.24]) with mapi id 14.03.0294.000; 9, 38 -- Sat, 27 Aug 2016 11:55:23 +1200 9, 38 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 9, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 38 -- Subject: Early career fibre structure scientist position in New Zealand 9, 38 -- Thread-Topic: Early career fibre structure scientist position in New Zealand 9, 38 -- Thread-Index: AdH/7Ytl8XvH3dClS/6okE2MQROGvg== 9, 38 -- Date: Fri, 26 Aug 2016 23:55:22 +0000 9, 38 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF1011FD8BE96-at-ruaexcpv01.agresearch.co.nz} 9, 38 -- Accept-Language: en-GB, en-NZ, en-US 9, 38 -- Content-Language: en-GB 9, 38 -- X-MS-Has-Attach: 9, 38 -- X-MS-TNEF-Correlator: 9, 38 -- x-originating-ip: [147.158.130.77] 9, 38 -- Content-Type: text/plain; charset="iso-8859-1" 9, 38 -- MIME-Version: 1.0 9, 38 -- Content-Transfer-Encoding: 8bit 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u7QNsS2h007296 ==============================End of - Headers==============================
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: U Alaska Fairbanks
Title-Subject: [Filtered] Auxiliary Gas for FEI Quanta 200 (older version)
Message: I'd like to run with nitrogen at in the low vac (1 Torr) range but am having a heck of a time getting a stable pressure, with the result being constant changes is the image brightness. If anyone has a solution or a regulator setup that works I'd like to know about it so I can "copy exact."
The system is perfectly stable when using water vapor so I don't think it is a problem with the instrument.
Thanks all!
Ken Severin University of Alaska Fairbanks
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We have a Quanta FEG 250 from about 6 years ago. We have the nitrogen vent line teed into the auxiliary gas feed. We have the two-stage regulator set to 1 psi or less. We don't have troubles as you describe. You didn't mention what pressure you have set. I would wonder if the regulator itself has gotten flaky. I would try changing it out for another. I also trust that your nitrogen tank is not near empty.
I can certainly relate to what you mention about the brightness being very sensitive to the pressure level. We've done some work with the Peltier stage on ours and found that we are better off controlling the temperature to change the humidity and get condensation than trying to control pressure. The temperature is better controlled and does not effect brightness.
I can't say I know how FEI runs their control system. It seems they could get by with a single control valve and just choose between the gas source. If they do, then I suppose the variability using N2 could be due to a leak in the tubing somewhere or a leaky solenoid valve. I would probably leave that up to an engineer to troubleshoot.
We had a different brand of VP-SEM that used to have trouble settling in on the set pressure. We could watch the control servo keep cranking the needle valve back and forth. It never settled down. To me, that suggested a control circuit issue - that the system was overcontrolling. Indeed, an intrepid service engineer was able to change out a component in the control board to get more ideal control. He no longer had to come back yearly to replace the valve when it seized up from the constant turning.
Hope it helps some, Warren Straszheim Materials Analysis and Research Lab Iowa State Univeristy
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X-from: kpseverin-at-alaska.edu
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: U Alaska Fairbanks
Title-Subject: [Filtered] Auxiliary Gas for FEI Quanta 200 (older version)
Message: I'd like to run with nitrogen at in the low vac (1 Torr) range but am having a heck of a time getting a stable pressure, with the result being constant changes is the image brightness. If anyone has a solution or a regulator setup that works I'd like to know about it so I can "copy exact."
The system is perfectly stable when using water vapor so I don't think it is a problem with the instrument.
Thanks all!
Ken Severin University of Alaska Fairbanks
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My Angel who repairs the electronic failures of my SEM for free, has created a stopwatch for counting the SEM filament time (we couldn't find anything similar on the web). The timer is simple and aesthetic and whoever is interested can download it at:
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Title-Subject: [Filtered] Project Scientist Series -Electron Microscopy Scientific Staff
Message: Dear Microscopists in Cryo-TEM: You might see the similar announcement a few months ago. Last announcement that looked for two TEM specialists resulted in the hiring of one staff member in materials science. Because of policy reason, the another position for cryo-TEM specialist for biological and soft materials was closed before the formal review of the candidates. If you are still interested in the TEM scientific staff position for cryo-TEM specialist for biological and soft materials, please resubmit your application. Surely, new submission is warmly welcome. Thanks,
Jian-Guo Zheng IMRI, UCI
Project Scientist Series -Electron Microscopy Scientific Staff
Salary-Commensurate with experience POSTING DATE: August 22, 2016 closing date: open until filled DESCRIPTION Manage the day-to-day operations and help guide the strategic build-out of the new transmission electron microscopy (TEM) facility at the UC Irvine Materials Research Institute (IRMI). IMRI has a large and growing user community of over 300 academic and industry users, with advanced materials characterization instruments that include state-of-the-art TEMs, scanning electron microscopes (SEMs), focused ion beam (FIB) systems, and a Kratos AXIS Supra surface science instrument (for details see http://lexi.eng.uci.edu). IMRI has recently built a new TEM facility housing five major instruments, including four high-end TEMs (Nion UltraSTEM 200 HERMES, JEOL JEM-ARM300CF Grand ARM, JEM-2800, and JEM-2100F Cryo-TEM) and a dual-beam FIB (Tescan GAIA-3 XMH FIB-SEM). Both the Grand ARM and the 2100F Cryo-TEM will be equipped with Gatans K2 cameras. IMRI is also equipped with a variety of TEM holders and specimen preparation tools. A TEM scientific staff position for cryo-TEM specialist for biological and soft materials is available. The successful applicants must hold PhD or equivalent degrees in the areas of Biology, Medicine, Materials Science, Physics, or Chemistry, and be TEM experts with at least five years of in-depth, hands-on experience with cryo-TEM imaging, data analysis, biological sample preparation, and maintenance of TEM equipment. The applicant must have mastery of modern TEM instrumentation and methods, data collection, analysis, and interpretation. Candidates with experience operating user facilities and expertise with advanced TEM techniques are of particular interest. Duties include: operate, maintain, and improve the TEMs; work with the IMRI leadership to identify and obtain accessories and additional equipment for a phased build-out of the TEM facility; train, supervise, and work directly with users to carry out research; co-manage all aspects of day-to-day IMRI operations; participate in teaching and outreach activities; promote IMRI on and off campus; secure grants, perform original research, attend scientific conferences, and publish scientific research in peer-reviewed journals.
The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy. TO LEARN MORE AND APPLY Apply by submitting your application to our online RECRUIT system at: https://recruit.ap.uci.edu/apply/JPF03682
REQUIREMENTS:
DOCUMENTS Cover Letter Curriculum Vitae - Your most recently updated C.V. Statement of Research Statement of Teaching (Optional) Statement of Contributions to Diversity - Statement addressing how past and/or potential contributions to diversity will advance UCI's Commitment to Inclusive Excellence. (Optional) Misc / Additional (Optional)
REFERENCES 3-5 letters of reference required
HOW TO APPLY 1. Create an ApplicantID 2. Provide required information and documents
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Message: We recently started using a processor for dehydration and initial infiltration steps. We are processing very large samples-1 to 1.5 mm. Since trying the processor, we have gotten poorly infiltrated samples, mainly in the center of the block. This happened even after we doubled the dehydration times. We use ethanol series followed by acetonitrile as an intermediate and Hard Plus resin for embedding. We only do a 2p solvent to 1 Part resin in the processor. We don't have this problem with bench processing.
Since our protocol is 9 days long, a processor for dehydration really helps. I'd really like to be able to use the processor for this. and infiltration.
Any troubleshooting ideas or suggestions are welcome. Thanks in advance,
JoAnn
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Title-Subject: [Filtered] Strange Pattern on SEM Images
Message: Hello everyone,
I have a question about a strange pattern that shows up on some of my SEM images. I have a SiOC specimen which does not have a good conductivity but since I need the specimen for other tests I do not want to coat it. As a result the specimen charges up under SEM and sometimes I can clearly see the zoom window being brighter than other areas. Until now everything seem reasonable but as soon as I click on auto brightness/contrast button, a strange pattern appears on the image. The observed pattern is similar to a few intermittent infinity symbols or something that you draw with a harmonograph. The pattern only shows up on this sample and not on the other ones. I was wondering if anyone has any idea about the origin of the observed pattern. I was not able to attach the image here but I am whiling to share it via e-mail. Thank you for your attention.
My best, Arezoo
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You are seeing residual charging from the beam as it is drawn during the auto brightness and contrast routine.
When you press the auto brightness and contrast button, your SEM likely does the following:
1) Draw a figure 8 and sample the signal from it within the field of view 2) Adjust contrast and brightness 3) Repeat from step 1 until acceptable contrast and brightness is achieved (output waveform min/max within parameters)
This allows the SEM to get a representative sample of the entire imaging area at an acceptable per pixel dwell time without having to collect a whole image.
-Jacob
On 16-08-31 04:26 PM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: arezoo.zare-at-okstate.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } arezoo.zare-at-okstate.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: arezoo.zare-at-okstate.edu Name: Arezoo Zare } } Organization: Oklahoma State University } } Title-Subject: [Filtered] Strange Pattern on SEM Images } } Message: Hello everyone, } } I have a question about a strange pattern that shows up on some of my SEM images. I have a SiOC } specimen which does not have a good conductivity but since I need the specimen for other tests I do } not want to coat it. As a result the specimen charges up under SEM and sometimes I can clearly see } the zoom window being brighter than other areas. Until now everything seem reasonable but as soon as } I click on auto brightness/contrast button, a strange pattern appears on the image. The observed } pattern is similar to a few intermittent infinity symbols or something that you draw with a } harmonograph. The pattern only shows up on this sample and not on the other ones. I was wondering if } anyone has any idea about the origin of the observed pattern. I was not able to attach the image } here but I am whiling to share it via e-mail. Thank you for your attention. } } My best, } Arezoo } } Login Host: 139.78.252.128 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
-- Jacob Kabel Electron Microscopist Department of Materials Engineering The University of British Columbia 6350 Stores Road, Room 419 Vancouver, British Columbia Canada V6T 1Z4 Phone: (604) 822-5648 Fax: (604) 822-3619 http://emlab.mtrl.ubc.ca/
==============================Original Headers============================== 10, 33 -- From jacob.kabel-at-ubc.ca Wed Aug 31 18:40:52 2016 10, 33 -- Received: from vmaprod1.mail-relay.ubc.ca (vmaprod1.mail-relay.ubc.ca [142.103.117.132]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u7VNeqWm010254 10, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Aug 2016 18:40:52 -0500 10, 33 -- Received: from vmaprod1.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 10, 33 -- by localhost (Email Security Appliance) with SMTP id D9B76C32A7_7C76AFBB; 10, 33 -- Wed, 31 Aug 2016 23:40:43 +0000 (GMT) 10, 33 -- Received: from mx3.mail-relay.ubc.ca (lb_mx-out.mail-relay.ubc.ca [10.92.8.24]) 10, 33 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 33 -- (Client CN "mx3.mail-relay.ubc.ca", Issuer "mx3.mail-relay.ubc.ca" (not verified)) 10, 33 -- by vmaprod1.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id 80123C05CC_7C76AFBF; 10, 33 -- Wed, 31 Aug 2016 23:40:43 +0000 (GMT) 10, 33 -- Received: from smtp.mail.ubc.ca (s-itsv-hub03p.ead.ubc.ca [137.82.151.73]) 10, 33 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 10, 33 -- (Client did not present a certificate) 10, 33 -- by mx3.mail-relay.ubc.ca (Postfix) with ESMTPS id 6C53015F685; 10, 33 -- Wed, 31 Aug 2016 16:40:43 -0700 (PDT) 10, 33 -- Received: from [137.82.16.20] (137.82.16.20) by smtp.mail.ubc.ca 10, 33 -- (137.82.151.73) with Microsoft SMTP Server id 14.3.301.0; Wed, 31 Aug 2016 10, 33 -- 16:40:42 -0700 10, 33 -- Subject: Re: [Microscopy] viaWWW:Strange Pattern on SEM Images 10, 33 -- To: {arezoo.zare-at-okstate.edu} , {Microscopy-at-microscopy.com} 10, 33 -- References: {201608312326.u7VNQMvT008725-at-ns.microscopy.com} 10, 33 -- From: Jacob Kabel {jacob.kabel-at-ubc.ca} 10, 33 -- Message-ID: {0c77a3b7-5feb-ab5d-b792-29165720cfb3-at-ubc.ca} 10, 33 -- Date: Wed, 31 Aug 2016 16:40:42 -0700 10, 33 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:45.0) Gecko/20100101 10, 33 -- Thunderbird/45.2.0 10, 33 -- MIME-Version: 1.0 10, 33 -- In-Reply-To: {201608312326.u7VNQMvT008725-at-ns.microscopy.com} 10, 33 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 10, 33 -- Content-Transfer-Encoding: 7bit 10, 33 -- X-Originating-IP: [137.82.16.20] ==============================End of - Headers==============================
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Hi Arezoo,
You are seeing residual charging from the beam as it is drawn during the auto brightness and contrast routine.
When you press the auto brightness and contrast button, your SEM likely does the following:
1) Draw a figure 8 and sample the signal from it within the field of view 2) Adjust contrast and brightness 3) Repeat from step 1 until acceptable contrast and brightness is achieved (output waveform min/max within parameters)
This allows the SEM to get a representative sample of the entire imaging area at an acceptable per pixel dwell time without having to collect a whole image.
-Jacob
On 16-08-31 04:26 PM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: arezoo.zare-at-okstate.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } arezoo.zare-at-okstate.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: arezoo.zare-at-okstate.edu Name: Arezoo Zare } } Organization: Oklahoma State University } } Title-Subject: [Filtered] Strange Pattern on SEM Images } } Message: Hello everyone, } } I have a question about a strange pattern that shows up on some of my SEM images. I have a SiOC } specimen which does not have a good conductivity but since I need the specimen for other tests I do } not want to coat it. As a result the specimen charges up under SEM and sometimes I can clearly see } the zoom window being brighter than other areas. Until now everything seem reasonable but as soon as } I click on auto brightness/contrast button, a strange pattern appears on the image. The observed } pattern is similar to a few intermittent infinity symbols or something that you draw with a } harmonograph. The pattern only shows up on this sample and not on the other ones. I was wondering if } anyone has any idea about the origin of the observed pattern. I was not able to attach the image } here but I am whiling to share it via e-mail. Thank you for your attention. } } My best, } Arezoo } } Login Host: 139.78.252.128 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
-- Jacob Kabel Electron Microscopist Department of Materials Engineering The University of British Columbia 6350 Stores Road, Room 419 Vancouver, British Columbia Canada V6T 1Z4 Phone: (604) 822-5648 Fax: (604) 822-3619 http://emlab.mtrl.ubc.ca/
==============================Original Headers============================== 10, 33 -- From jacob.kabel-at-ubc.ca Wed Aug 31 18:40:52 2016 10, 33 -- Received: from vmaprod1.mail-relay.ubc.ca (vmaprod1.mail-relay.ubc.ca [142.103.117.132]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u7VNeqWm010254 10, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Aug 2016 18:40:52 -0500 10, 33 -- Received: from vmaprod1.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 10, 33 -- by localhost (Email Security Appliance) with SMTP id D9B76C32A7_7C76AFBB; 10, 33 -- Wed, 31 Aug 2016 23:40:43 +0000 (GMT) 10, 33 -- Received: from mx3.mail-relay.ubc.ca (lb_mx-out.mail-relay.ubc.ca [10.92.8.24]) 10, 33 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 33 -- (Client CN "mx3.mail-relay.ubc.ca", Issuer "mx3.mail-relay.ubc.ca" (not verified)) 10, 33 -- by vmaprod1.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id 80123C05CC_7C76AFBF; 10, 33 -- Wed, 31 Aug 2016 23:40:43 +0000 (GMT) 10, 33 -- Received: from smtp.mail.ubc.ca (s-itsv-hub03p.ead.ubc.ca [137.82.151.73]) 10, 33 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 10, 33 -- (Client did not present a certificate) 10, 33 -- by mx3.mail-relay.ubc.ca (Postfix) with ESMTPS id 6C53015F685; 10, 33 -- Wed, 31 Aug 2016 16:40:43 -0700 (PDT) 10, 33 -- Received: from [137.82.16.20] (137.82.16.20) by smtp.mail.ubc.ca 10, 33 -- (137.82.151.73) with Microsoft SMTP Server id 14.3.301.0; Wed, 31 Aug 2016 10, 33 -- 16:40:42 -0700 10, 33 -- Subject: Re: [Microscopy] viaWWW:Strange Pattern on SEM Images 10, 33 -- To: {arezoo.zare-at-okstate.edu} , {Microscopy-at-microscopy.com} 10, 33 -- References: {201608312326.u7VNQMvT008725-at-ns.microscopy.com} 10, 33 -- From: Jacob Kabel {jacob.kabel-at-ubc.ca} 10, 33 -- Message-ID: {0c77a3b7-5feb-ab5d-b792-29165720cfb3-at-ubc.ca} 10, 33 -- Date: Wed, 31 Aug 2016 16:40:42 -0700 10, 33 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:45.0) Gecko/20100101 10, 33 -- Thunderbird/45.2.0 10, 33 -- MIME-Version: 1.0 10, 33 -- In-Reply-To: {201608312326.u7VNQMvT008725-at-ns.microscopy.com} 10, 33 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 10, 33 -- Content-Transfer-Encoding: 7bit 10, 33 -- X-Originating-IP: [137.82.16.20] ==============================End of - Headers==============================
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Message: We recently started using a processor for dehydration and initial infiltration steps. We are processing very large samples-1 to 1.5 mm. Since trying the processor, we have gotten poorly infiltrated samples, mainly in the center of the block. This happened even after we doubled the dehydration times. We use ethanol series followed by acetonitrile as an intermediate and Hard Plus resin for embedding. We only do a 2p solvent to 1 Part resin in the processor. We don't have this problem with bench processing.
Since our protocol is 9 days long, a processor for dehydration really helps. I'd really like to be able to use the processor for this. and infiltration.
Any troubleshooting ideas or suggestions are welcome. Thanks in advance,
JoAnn
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Email: marco.cantoni-at-epfl.ch Name: Marco Cantoni
Organization: EPFL-CIME
Title-Subject: [Filtered] SSOM 3D Symposium 2016
Message: Abstract and registration deadline is approaching! Check the updated program. Dont miss this event. SSOM Interdisciplinary Symposium on 3D Microscopy 2016 in Les Diablerets. http://cime.epfl.ch/3D-Symposium2016
Best regards
Marco Cantoni
______________________________ Dr. Marco Cantoni EPFL-SB-CIME Bat. MXC-133, Station 12 1015 Lausanne, Switzerland phone (office and mobile): +41 21 6934816 fax: +41 21 6934401 ______________________________
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Email: studentstef-at-googlemail.com Name: Stefan
Organization: University
Title-Subject: [Filtered] X-Ray Shielding in SEM
Message: Hallo everyone,
I have a question about X-Ray shielding in a scanning electron microscope and I hope you can help me! The topic is that when electrons hit the specimen, characteristic radiation up to 10keV is generated. This radiation is blocked/decelerated my the surrounding tower and chamber that are shielded with mu-metal (how thick is this coating). If I would just use pure Aluminium the X-Ray shielding wouldn't be enough. So far, is this correct? Because Aluminium has a half-value thickness of about 112mm for 10keV x-radiation. I am a bit concerned that some new-built parts of our SEM-chamber-cover aren't enough to shield the generated x-radiation. They are made out of alluminium with a thin mu-metal coating. It may be enough for magnetic field but I don't know if it is enough for the x-rays.. Can anyone tell me if my concerns are legitimate or is the X-radiation easily shielded with such layers?
Thank you for your help!
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Title-Subject: [Filtered] Thank you for responses infiltration/dehydration issues
Message: Dear listers. i appreciate all the responses I got to my question. After I posted, I took a closer look at the instrument and saw that the caps were not aligned properly with the vials, causing a gap when the lid was lowered.
I sent an image to the company and it was quickly ascertained that it is likely a defect in the equipment. Hopefully, our problems will be solved by having the lid repaired or the unit replaced. JoAnn
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Email: sripad.ram-at-gmail.com Name: Sripad Ram
Organization: Quantitative BioImaging Conference
Title-Subject: [Filtered] Call for abstracts: 2017 Quantitative BioImaging Conference
Message: Can you please post the following conference announcement in the listerv?
Dear colleague, After a successful meeting at Delft, The Netherlands in 2016, we are pleased to announce that the 5th international quantitative bioimaging conference will be held on Jan 5- 7, 2017 at Texas A&M University, College Station, TX. The upcoming conference will continue to focus on the quantitative analysis of bioimaging data in an interdisciplinary manner. Abstract submission: We seek contributions in any area of quantitative microscopy. Presentations that demonstrate in detail new approaches are particularly welcome, including but not limited to algorithmic and software developments, physical modeling approaches etc. The use of quantitative imaging techniques in biological applications is also of great interest. For submission details please see the conference website (www.QuantitativeBioImaging.com).
ABSTRACTS ARE DUE BY SEPTEMBER 15, 2016
Key note speakers: Ernst Stelzer, Buchmann Institute for Molecular Life Sciences, Frankfurt, Germany Marino Zerial, Max Planck Institute Dresden, Germany Robert Singer, Albert Einstein College of Medicine, New York, USA
Invited Speakers (confirmed): C. Calderon (Denver, USA); C. Cogswell (Boulder, USA); S. Cox (London, UK); Y. Kalaidzidis (Dresden, Germany); P. Kner (Athens, Georgia, USA); U. Kubitscheck (Bonn, Germany); E. Lemke (Heidelberg, Germany); K. Lidke (Albuquerque, NM, USA); J. Liphardt (Stanford, USA); J-C. Olivo-Marin (Paris, France); B. Rieger (Delft, The Netherlands); J. Rittscher (Oxford, UK); N. Stuurman (San Francisco, USA); G. Rohde (Charlottesville, VA, USA); K. Rohr (Heidelberg, Germany); Y. Shechtman (Stanford, USA); S. Stallinga (Delft, The Netherlands); N. Thompson (Chapel Hill, HC, USA); W. Wiegraebe (Seattle, USA).
Registration fees: In line with prior years, there will be no registration fees for academic attendees. We look forward to seeing you in College Station in January. Sincerely, The organizers of QBI2017
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X-rays up to the primary electron beam energy are generated inside the SEM - if that is 30 kev, then some of the X-rays generated will be at 30 keV. If it’s 10 keV, then X-rays to 10 keV will be generated.
Mu-metal is for stopping stray external electromagnetic fields influencing the SEM incident electron beam - it has nothing directly to do with X-ray shielding, although it will obviously absorb some X-rays but that isn’t its primary purpose. For optimum shielding, the mu-metal needs to be on the outside of the chamber/column in which position it really isn’t going to be of any consequence for X-ray shielding - if the X-rays have got that far, you have a real problem.
Aluminium is used to stop electrons - 30 keV electrons hitting Al generate Al X-rays, predominatly at around 1.48 keV, which are easily absorbed. 30 keV electrons hitting steel are going to generate mainly Fe X-rays at around 7 kev - not so easy to absorb. Hitting lead, they generate Pb L X-rays at up to 12 keV, more difficult still to absorb.
I would suggest that an Al-only port cover for an SEM is not acceptable from a radiation safety viewpoint. Have a look at the other port covers on the SEM chamber and replicate them in design, materials and thickness and even if you are only using the SEM at up to 10 keV, consider that it is probably capable of 30 keV? A straightforward 15 mm thick steel plate is probably going to do the job. For something more sophisticated, 2 mm of Al on the inside and some mu-metal on the outside.
Larry Stoter
} On 3 Sep 2016, at 14:42, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: studentstef-at-googlemail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } studentstef-at-googlemail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: studentstef-at-googlemail.com Name: Stefan } } Organization: University } } Title-Subject: [Filtered] X-Ray Shielding in SEM } } Message: Hallo everyone, } } I have a question about X-Ray shielding in a scanning electron microscope and I hope you can help } me! The topic is that when electrons hit the specimen, characteristic radiation up to 10keV is } generated. This radiation is blocked/decelerated my the surrounding tower and chamber that are } shielded with mu-metal (how thick is this coating). If I would just use pure Aluminium the X-Ray } shielding wouldn't be enough. So far, is this correct? } Because Aluminium has a half-value thickness of about 112mm for 10keV x-radiation. I am a bit } concerned that some new-built parts of our SEM-chamber-cover aren't enough to shield the generated } x-radiation. They are made out of alluminium with a thin mu-metal coating. It may be enough for } magnetic field but I don't know if it is enough for the x-rays.. Can anyone tell me if my concerns } are legitimate or is the X-radiation easily shielded with such layers? } } Thank you for your help!
==============================Original Headers============================== 8, 48 -- From larry.stoter-at-gmail.com Sat Sep 3 09:10:14 2016 8, 48 -- Received: from mail-wm0-f43.google.com (mail-wm0-f43.google.com [74.125.82.43]) 8, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u83EAEQp026875 8, 48 -- for {Microscopy-at-microscopy.com} ; Sat, 3 Sep 2016 09:10:14 -0500 8, 48 -- Received: by mail-wm0-f43.google.com with SMTP id w2so65653114wmd.0 8, 48 -- for {Microscopy-at-microscopy.com} ; Sat, 03 Sep 2016 07:10:15 -0700 (PDT) 8, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 48 -- d=gmail.com; s=20120113; 8, 48 -- h=mime-version:subject:from:in-reply-to:date 8, 48 -- :content-transfer-encoding:message-id:references:to; 8, 48 -- bh=AULZM/NQ8OHXVT7BuIj5E9jHIUaQhNx20mADJcEht0s=; 8, 48 -- b=iiQkWflOYXPR1X7WO1LObx7r8CaDLvOm9Vvj3M/ySsZkyxbd9zhTfuNShNaY+0xMM4 8, 48 -- nyXDc5Ju88kvlbPDPPPm2DL59BGbIMNO5iNh2F3gjlDp5zvILS6B2lysV4vgYjT0L2N0 8, 48 -- pxveIz+dhd87Kxcoo49mNlKho9u/Oax8jC2MsBkberzdM1HHv9bkxdWNdMArL2+d0Iuh 8, 48 -- STvLnHDm+Zqrr5SLldWkSE2bP4QZD9x8pAUZNRjQYyhXCAlNAFNFaUgRIkz0dz6y9f2P 8, 48 -- p8N7JpnGHjLssAz8FdfRRKQqRhB5OfASBkD3xYYfZe46HklghFVR3EK2gIfdkECI4Czw 8, 48 -- Ju+Q== 8, 48 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 48 -- d=1e100.net; s=20130820; 8, 48 -- h=x-gm-message-state:mime-version:subject:from:in-reply-to:date 8, 48 -- :content-transfer-encoding:message-id:references:to; 8, 48 -- bh=AULZM/NQ8OHXVT7BuIj5E9jHIUaQhNx20mADJcEht0s=; 8, 48 -- b=NNpBzhYSKyYuxXtqTSZLdKD1ZR0KvVL5ru+V/t2xwIckjYm5WLnLEY43xMa3HT4a7s 8, 48 -- 9YSUmiMJxWG4TwOlLRlPKpOTagOrUpPXbVveCUixBlPkGp1OQWb8mw/28kvPUCs2mAe6 8, 48 -- Tuss1KgGxeETsWRlZMJcuPUA++gMvh9DIWds74fQ5UD8xPCgR8oDkWCDZfUQcYnwynOm 8, 48 -- DVoRR1SZ4hmNI8veQZRCP3j6LNErIVwjzo3xa6wREpov0BOwXYz7wtYGo1TgSasrysAQ 8, 48 -- R4g14F4lsGumWBeyqCQh+Jk9TP1/TdhxQStZC8cABHv+zd7X2GhYH5xzQ6dW6e39DybG 8, 48 -- mEwA== 8, 48 -- X-Gm-Message-State: AE9vXwNkzCr2RklA0c1DHSVSp3/GHkzj0JpKwpnFfgyHxOzophs29UnX8G/UWmZSk51zBg== 8, 48 -- X-Received: by 10.28.47.199 with SMTP id v190mr7510591wmv.28.1472911815283; 8, 48 -- Sat, 03 Sep 2016 07:10:15 -0700 (PDT) 8, 48 -- Received: from [10.175.0.2] (host86-169-169-48.range86-169.btcentralplus.com. [86.169.169.48]) 8, 48 -- by smtp.gmail.com with ESMTPSA id h7sm15713645wjd.17.2016.09.03.07.10.14 8, 48 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 8, 48 -- Sat, 03 Sep 2016 07:10:14 -0700 (PDT) 8, 48 -- Content-Type: text/plain; charset=utf-8 8, 48 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 8, 48 -- Subject: Re: [Microscopy] viaWWW:X-Ray Shielding in SEM 8, 48 -- From: Larry Stoter {larry.stoter-at-gmail.com} 8, 48 -- In-Reply-To: {201609031342.u83DgqDZ001617-at-ns.microscopy.com} 8, 48 -- Date: Sat, 3 Sep 2016 15:09:32 +0100 8, 48 -- Message-Id: {2B9A3022-5406-4C85-9493-8EFA1E9D82A1-at-gmail.com} 8, 48 -- References: {201609031342.u83DgqDZ001617-at-ns.microscopy.com} 8, 48 -- To: MSA Microscopy List {Microscopy-at-microscopy.com} , 8, 48 -- studentstef-at-googlemail.com 8, 48 -- X-Mailer: Apple Mail (2.3124) 8, 48 -- Content-Transfer-Encoding: 8bit 8, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u83EAEQp026875 ==============================End of - Headers==============================
I was asked to spread the word about an EM Researcher position at the National Centre for Cellular Imaging at the University of Gothenburg, Sweden. Details about the institution, the job profile, and the required qualification can be found at:
Dear colleagues, This is slightly off-topic for this microscopy list, but I would appreciate your help in sharing this job announcement with your faculty, post-docs, and graduate students who might be interested. Thank you, Stefanie
FACULTY POSITION IN SUSTAINABILITY SCIENCE The Department of Earth and Environmental Studies invites applications for a full-time (10-month) tenure-track Assistant Professor position in Sustainability Science. Applicants with expertise in areas including but not limited to urban sustainability, urban design and regional planning, climate change mitigation and adaptation, industrial ecology, renewable energy, carbon auditing, and life cycle assessment, are encouraged to apply. The successful candidate will develop a vigorous externally funded research program and have a strong commitment to excellence in teaching. The candidate will be expected to play a critical role in our bachelors, masters, and professional science masters programs in Sustainability Science, and contribute to teaching and mentoring in our Geography, Earth and Environmental Sciences, and Environmental Management bachelors, masters, and doctoral programs. Teaching responsibilities will include introductory courses as well as upper-level undergraduate and graduate courses within the applicants areas of expertise. Service to the department, university, and larger professional community is also expected.
QUALIFICATIONS:Applicants are expected to have a Ph.D. in sustainability science, environmental science, urban planning, geosciences, geography, resource management, or other appropriate field, a record of peer-reviewed scholarship in sustainability, and evidence of potential for success in grant activity. Post-doctoral experience is desirable.
SALARY RANGE: Salary and range is dependent upon qualifications STARTING DATE: September 1, 2017
APPLICATIONS:Electronic applications assembled into a single combined .pdf or .doc file and containing a cover letter, CV, statements of teaching and research interests, and the names and contact information for three professional references should be sent to EAESsearch-at-mail.montclair.edu. Applications are due byOctober 15, 2016.
********************* Dr. Stefanie A. Brachfeld Professor and Chair,Department of Earth & Environmental Studies Director, PhD Program in Environmental Management Montclair State University Montclair, NJ 07043 phone: (973) 655-5129 fax: 973-655-4072 brachfelds-at-mail.montclair.edu *********************
==============================Original Headers============================== 7, 29 -- From brachfelds-at-mail.montclair.edu Sun Sep 4 13:24:55 2016 7, 29 -- Received: from smtp.montclair.edu (smtp.montclair.edu [130.68.1.64]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u84IOsOV024038 7, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 4 Sep 2016 13:24:54 -0500 7, 29 -- Received: from convergence2.montclair.edu (unknown [130.68.1.64]) 7, 29 -- by smtp.montclair.edu (Postfix) with ESMTP id 5A4B9214F1 7, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 4 Sep 2016 14:25:00 -0400 (EDT) 7, 29 -- MIME-version: 1.0 7, 29 -- Content-disposition: inline 7, 29 -- Content-type: text/plain; charset=windows-1252 7, 29 -- Received: from montclair.edu ([127.0.0.1]) by convergence2.montclair.edu 7, 29 -- (Oracle Communications Messaging Server 7u4-25.01(7.0.4.25.0) 64bit (built Feb 7, 29 -- 29 2012)) with ESMTPA id {0OCZ00A99SHOEY00-at-convergence2.montclair.edu} for 7, 29 -- Microscopy-at-microscopy.com; Sun, 04 Sep 2016 14:25:00 -0400 (EDT) 7, 29 -- Received: from [127.0.0.1] (Forwarded-For: 76.117.178.61) 7, 29 -- by convergence2.montclair.edu (mshttpd); Sun, 04 Sep 2016 14:25:00 -0400 7, 29 -- From: "Stefanie A. Brachfeld" {brachfelds-at-mail.montclair.edu} 7, 29 -- To: Microscopy-at-microscopy.com 7, 29 -- Message-id: {b9531761752469f8.57cc2ebc-at-montclair.edu} 7, 29 -- Date: Sun, 04 Sep 2016 14:25:00 -0400 7, 29 -- X-Mailer: Oracle Communications Messenger Express 7u4-25.01(7.0.4.25.0) 64bit 7, 29 -- (built Feb 29 2012) 7, 29 -- Content-language: en 7, 29 -- Subject: Tenure Track Position in Sustainability Science at Montclair State 7, 29 -- University 7, 29 -- X-Accept-Language: en 7, 29 -- Priority: normal 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u84IOsOV024038 ==============================End of - Headers==============================
Does anyone out there have a good protocol for staining 1 micron thick resin sections (in my case it is Araldite), using Hematoxylin and Eosin?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 36 -- From tbargar-at-unmc.edu Tue Sep 6 15:31:06 2016 7, 36 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u86KV6ha029942 7, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2016 15:31:06 -0500 7, 36 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 36 -- by Outbound.unmc.edu (Proprietary) with SMTP id 025EA2A16E1 7, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2016 15:01:06 -0500 (CDT) 7, 36 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 36 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 7, 36 -- (No client certificate requested) 7, 36 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id 42D962A16DC 7, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2016 15:01:04 -0500 (CDT) 7, 36 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 36 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 36 -- 14.03.0301.000; Tue, 6 Sep 2016 15:31:17 -0500 7, 36 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 36 -- Subject: a H & E protocol for 1 micron thick araldite sections 7, 36 -- Thread-Topic: a H & E protocol for 1 micron thick araldite sections 7, 36 -- Thread-Index: AdIIfTi1GAoWgRM8SjyeEM6WzAXXtA== 7, 36 -- Date: Tue, 6 Sep 2016 20:31:16 +0000 7, 36 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0B5AFB3B8-at-UNMCEX2.unmcresforest.org} 7, 36 -- Accept-Language: en-US 7, 36 -- Content-Language: en-US 7, 36 -- X-MS-Has-Attach: 7, 36 -- X-MS-TNEF-Correlator: 7, 36 -- x-originating-ip: [10.8.64.15] 7, 36 -- Content-Type: text/plain; charset="us-ascii" 7, 36 -- MIME-Version: 1.0 7, 36 -- X-VPM-MSG-ID: aa993c2f-21ab-4460-bab2-7c99b07ae261 7, 36 -- X-VPM-HOST: zixvpm02.unmc.edu 7, 36 -- X-VPM-GROUP-ID: 2dad33f6-32ad-4f0f-9262-f37ec8d9547a 7, 36 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 36 -- X-VPM-IS-HYBRID: 0 7, 36 -- Content-Transfer-Encoding: 8bit 7, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u86KV6ha029942 ==============================End of - Headers==============================
just as a quick shot....therefore hopefully as short as acceptable in Listserver communication:
as to my knowledge H&E on (hydrophobic) Araldite (or also Epon/epoxy semithin) resin sections can be done only after harsh chemical treatment (like either etching, or - sometimes also removal of resin)...i. e. use of oxidants (like H2O2, or epoxy "solvents" like sodium (or potassium-)methylate
Google.com Search for: {Araldite AND resin AND section AND staining AND H&E OR hematoxylin OR haematoxylin AND eosin} reveals many references.... Google.com Search for: {Araldite AND resin AND section AND staining AND H&E OR hematoxylin OR haematoxylin AND eosin AND ETCHING} approx. 925 results Search https://scholar.google.com: for: {Araldite AND resin AND section AND staining AND H&E OR hematoxylin OR haematoxylin AND eosin} approx. 2950 results Search https://scholar.google.com: for: { Araldite AND resin AND section AND staining AND H&E OR hematoxylin OR haematoxylin AND eosin AND ETCHING} approx.117 results
Perhaps as a {standard} recipe (which might not be very successful for several reasons...: Application of standard micro-anatomical staining methods to epoxy resin-embedded sections S. R. Aparicio and P. Marsden ( PMCID: PMC474254) J Clin Pathol. 1969 Sep; 22(5): 589592. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC474254/ or pdf: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC474254/pdf/jclinpath00382-0079.p df
and/or WARMKE H.E. & LEE S-L.J. : Improved staining procedures for semithin epoxy sections of plant tissues Stain Technol 51 No3, 179-185, 1976
Knowing that there has been described (by references) H&E-staining applied to resin sections which on serious insight turned out to be wrong descriptions or false supposition (e. g. naming of procedure)... Ending for now - but not at the End of knowledge... Best wishes-good luck- regards,
Wolfgang Muss PhD Retired, Member of MSA SALZBURG, AUSTRIA
-----Ursprngliche Nachricht----- Von: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Gesendet: Dienstag, 6. September 2016 23:04 An: wij.muss-at-aon.at Betreff: [Microscopy] a H & E protocol for 1 micron thick araldite sections
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Dear Listers,
Does anyone out there have a good protocol for staining 1 micron thick resin sections (in my case it is Araldite), using Hematoxylin and Eosin?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 36 -- From tbargar-at-unmc.edu Tue Sep 6 15:31:06 2016 7, 36 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u86KV6ha029942 7, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2016 15:31:06 -0500 7, 36 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 36 -- by Outbound.unmc.edu (Proprietary) with SMTP id 025EA2A16E1 7, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2016 15:01:06 -0500 (CDT) 7, 36 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 36 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 7, 36 -- (No client certificate requested) 7, 36 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id 42D962A16DC 7, 36 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Sep 2016 15:01:04 -0500 (CDT) 7, 36 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 36 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 36 -- 14.03.0301.000; Tue, 6 Sep 2016 15:31:17 -0500 7, 36 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 36 -- Subject: a H & E protocol for 1 micron thick araldite sections 7, 36 -- Thread-Topic: a H & E protocol for 1 micron thick araldite sections 7, 36 -- Thread-Index: AdIIfTi1GAoWgRM8SjyeEM6WzAXXtA== 7, 36 -- Date: Tue, 6 Sep 2016 20:31:16 +0000 7, 36 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0B5AFB3B8-at-UNMCEX2.unmcresforest.org} 7, 36 -- Accept-Language: en-US 7, 36 -- Content-Language: en-US 7, 36 -- X-MS-Has-Attach: 7, 36 -- X-MS-TNEF-Correlator: 7, 36 -- x-originating-ip: [10.8.64.15] 7, 36 -- Content-Type: text/plain; charset="us-ascii" 7, 36 -- MIME-Version: 1.0 7, 36 -- X-VPM-MSG-ID: aa993c2f-21ab-4460-bab2-7c99b07ae261 7, 36 -- X-VPM-HOST: zixvpm02.unmc.edu 7, 36 -- X-VPM-GROUP-ID: 2dad33f6-32ad-4f0f-9262-f37ec8d9547a 7, 36 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 36 -- X-VPM-IS-HYBRID: 0 7, 36 -- Content-Transfer-Encoding: 8bit 7, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u86KV6ha029942 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 23 -- From wij.muss-at-aon.at Wed Sep 7 04:44:11 2016 24, 23 -- Received: from bsmtp5.bon.at (bsmtp5.bon.at [195.3.86.187]) 24, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u879bsTo025627 24, 23 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2016 04:38:14 -0500 24, 23 -- Received: from bsmtp3.bon.at (unknown [192.168.181.102]) 24, 23 -- by bsmtp5.bon.at (Postfix) with ESMTPS id 3sTdSK5k6bz5v2p 24, 23 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2016 11:28:33 +0200 (CEST) 24, 23 -- Received: from MussTHINK (unknown [93.83.25.98]) 24, 23 -- by bsmtp3.bon.at (Postfix) with ESMTPSA id 3sTdSK00gkz5tln; 24, 23 -- Wed, 7 Sep 2016 11:28:32 +0200 (CEST) 24, 23 -- From: "MUSS Wolfgang SCUR Secretary" {wij.muss-at-aon.at} 24, 23 -- To: {microscopy-at-microscopy.com} 24, 23 -- Subject: [Microscopy] Re: a H & E protocol for 1 micron thick araldite sections 24, 23 -- Date: Wed, 7 Sep 2016 11:28:34 +0200 24, 23 -- Message-ID: {000001d208ea$353b8bb0$9fb2a310$-at-aon.at} 24, 23 -- MIME-Version: 1.0 24, 23 -- Content-Type: text/plain; 24, 23 -- charset="iso-8859-1" 24, 23 -- X-Mailer: Microsoft Outlook 15.0 24, 23 -- Thread-Index: AdII4lxFWyxC8ozSSOmClY2cFuJDEg== 24, 23 -- Content-Language: de 24, 23 -- Content-Transfer-Encoding: 8bit 24, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u879bsTo025627 ==============================End of - Headers==============================
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a cryo-ultramicrotomy mini-course on October 17th and 18th, 2016. The course is designed to teach advanced users of cryo-ultramicrotomy techniques. The format includes lectures, demonstrations and hands on practice. Participants must be familiar with room temperature ultramicrotome operation and techniques. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found in our website
A companion Immuno Gold Silver Staining (IGSS) workshop sponsored by EMS-Diatome and Aurion will be held at EMCIF on October 19th to 21st after the cryo ultramicrotomy minicourse. New to this years program, the workshop will also include Tokuyasu immunolabeling method. Individuals who are interested to learn Tokuyasu immunogold labeling techniques are strongly encouraged to participate both workshops. A $50 discount will be offered for registration of both courses.
For more information about the IGSS workshop, visit:
Please email coreimaging-at-umaryland.edu or visit our website http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/ for more information.
Thanks.
Ru-ching
Ru-ching Hsia, Ph.D. rhsia-at-umaryland.edu Associate Professor and Director Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
Does anyone know of a good way to export EDS spectrum images from the Noran System 7 software and then import them into Digital Micrograph? I’ve found a way to export the EDS data in raw format, but I don’t know how to load it into DM properly.
Any thoughts? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
we are using a older model SEM to image samples with copper structures on the surface and just a few nm below the surface at the same time (typically buried under a thin layer of glass). Since the copper layer below the surface is just covered by this very thin glass layer, even with lower acceleration voltages it is very hard to distinguish on the images whether the imaged copper structures are located on the surface or below it. As our goal would be that the copper on the surface clearly looks different from the copper buried under the thin glass layer, we were wondering whether we could use a staining procedure.
For instance, we are aware of a wet chemical procedure allowing to tin-plate copper. However, without trying it we don't know if it would result in a considerable different contrast in the SEM as the atomic mass of Sn is very close to the one of Cu. Wet chemical gold plating on the other hand involves not only expensive but also highly toxic chemicals.
Are you aware of (wet chemical) staining procedures for copper that produce a considerable contrast and are preferably inexpensive as well as non highly toxic ?
Is there maybe another technique we could use ?
Kind regards, Stefan
==============================Original Headers============================== 6, 20 -- From dev.c0debabe-at-gmail.com Wed Sep 7 12:07:22 2016 6, 20 -- Received: from mail.tbmn.org (mail.tbmn.org [87.118.84.39]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u87H7KSV020041 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Sep 2016 12:07:20 -0500 6, 20 -- Received: from [192.168.1.5] (80-108-8-29.cable.dynamic.surfer.at [80.108.8.29]) 6, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) 6, 20 -- (No client certificate requested) 6, 20 -- (Authenticated sender: mne-at-tbmn.org) 6, 20 -- by mail.tbmn.org (Postfix) with ESMTPSA id 59751276C262 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Sep 2016 19:07:35 +0200 (CEST) 6, 20 -- To: Microscopy-at-microscopy.com 6, 20 -- From: Stefan Schoenleitner {dev.c0debabe-at-gmail.com} 6, 20 -- Subject: Staining of Cu for SEM imaging 6, 20 -- Message-ID: {0aaaa747-f2e7-6478-f22b-ad672dc237c4-at-gmail.com} 6, 20 -- Date: Wed, 7 Sep 2016 19:07:34 +0200 6, 20 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 6, 20 -- Thunderbird/45.2.0 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; charset=utf-8 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone know of a good way to export EDS spectrum images from the Noran System 7 software and then import them into Digital Micrograph? I’ve found a way to export the EDS data in raw format, but I don’t know how to load it into DM properly.
Any thoughts? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
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Organization: Centers for Disease Control and Prevention
Title-Subject: [Filtered] Fungi
Message: Does anyone know of an ultrastructural atlas of fungi? Even if its old, I could probably find it online. Or, perhaps a fairly comprehensive manuscript?
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Email: n.i.clarke-at-warwick.ac.uk Name: Nick Clarke
Message: Hello, I was wondering if you could provide me with a good reference for immunogold spatial resolution i.e. distance from antigen to centre of gold particle. Any help would be really appreciated with this. Kind regards, Nick.
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Email: brad-at-quant-corp.com Name: Brad Lindner
Organization: Quant Corporation
Title-Subject: [Filtered] Hitachi S-2700 SEM
Message: I recently purchased a used Hitachi S-2700 SEM from a local university but do not have any of the user guides / manuals. Does anyone have these that would be willing to send in PDF form?
Additionally, I would like to verify that I've set up all of the components correctly before I turn the system on - are there any independent SEM/EDS service companies in the Milwaukee, WI area that I could hire for this and regular maintenance?
Thanks for your time, Brad
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Email: marie.cantino-at-gmail.com Name: Marie Cantino
Organization: University of Connecticut
Title-Subject: [Filtered] TEM of mouse neuromuscular junction
Message: I have been asked to help a student who would like to do TEM of mouse neuromuscular junctions (NMJ). Ive done plenty of TEM on muscle, but never looking specifically for NMJ. This strikes me as a bit of a needle in a haystack type of effort, since there is generally only one, or possibly a few per fiber, depending on the fiber type. Does anyone have experience with this? I see some studies use cholinesterase staining to find the NMJ first at the light level. Is this technique tricky? Are there other tricks i should know about? Will this require hundreds of hours of microtomy? Thanks for any help or advice you can offer.
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Does anybody have schematics or a service guide available for an older Noran ULGS-48 stage? I really just need the pinouts for the connections to the motors. I would like to replace the dying electronics with something a little more modern. It’s a 5-axis replacement stage in a JEOL 848.
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Email: lucy.avati-at-sydney.edu.au Name: Lucy Avati
Organization: University of Sydney
Title-Subject: [Filtered] Job opportunity: Microscopy Officer at the University of Sydney
Message: Microscopy Officer School of Medical Sciences - Bosch Institute Sydney Medical School Reference No. 1519/0916
Located at Camperdown Campus Part-time 0.5 FTE, fixed term for 12 months Remuneration package: $95K-$104K p.a. pro rata which includes leave loading and up to 17% super
The University of Sydney is Australia's first university and has an outstanding global reputation for academic and research excellence. It employs over 7600 permanent staff, supporting over 60,000 students.
The Bosch Institute is the research arm of the School of Medical Sciences, Sydney Medical School. Its role is to add value to the research activities of the SMS through the provision of well-managed core facilities that include new and emerging technologies and provide excellent training for junior scientists.
We are seeking a Microscopy Officer to provide direct support to the Advanced Microscopy Facility (AMF) Core Facilities Manager at the Bosch Institute.
You will: be a point of contact for the ZEISS AxioScan high-throughput digital slide-scanning microscope and provide a slide-scanning service for the Institute train students and researchers on the operation of the transmitted light, fluorescence and confocal microscopes in the Facility through individual and group teaching sessions carry out administrative duties including ordering, processing AMF registration and access and submission of grant applications.
You will need: PhD or equivalent soon to be awarded or thesis submitted may be acceptable, depending on other knowledge, skills, experience demonstrated experience having worked in a biomedical research environment experience with light, fluorescence and confocal microscopy experience with general administrative duties experience in teaching/demonstrating at tertiary level experience with publication of scientific papers in peer-reviewed journals knowledge of basic and advanced microscope methods experience with the operation of basic and advanced fluorescence microscopes ability to interact positively with members of the university community, including academic staff, research students, general staff understanding of computer operation, including software applications.
Highly desirable: experience with digital slide slide-scanning microscopy experience with laser microdissection and/or optical tweezers experience with multi-photon microscopy experience with preparation of applications to funding bodies experience with University of Sydney structure, environment, operations knowledge of image analysis and processing software
All applications must be submitted via the University of Sydney careers website. Visit sydney.edu.au/recruitment and search by the reference number for more information and to apply.
The University reserves the right not to proceed with any appointment.
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There are plans for several very large wind turbines close to our Tecnai and Titan TEM systems. There are reports about low frequency (1-10 Hz) ground vibrations generated by wind turbines. The typical passive dampening systems on TEM systems have a resonance frequency of around 7 Hz and then dampening starts.
I would be interested to hear about any experiences with wind turbines close to (~800 meters) high-end TEM systems.
Thanks in advance,
Wim
==============================Original Headers============================== 5, 39 -- From wim.hagen-at-me.com Thu Sep 8 21:31:25 2016 5, 39 -- Received: from pv33p04im-asmtp002.me.com (pv33p04im-asmtp002.me.com [17.143.181.11]) 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u892VNZr004856 5, 39 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Sep 2016 21:31:24 -0500 5, 39 -- Received: from process-dkim-sign-daemon.pv33p04im-asmtp002.me.com by 5, 39 -- pv33p04im-asmtp002.me.com 5, 39 -- (Oracle Communications Messaging Server 7.0.5.38.0 64bit (built Feb 26 2016)) 5, 39 -- id {0OD600E00SIIJ100-at-pv33p04im-asmtp002.me.com} for Microscopy-at-microscopy.com; 5, 39 -- Thu, 08 Sep 2016 13:30:51 +0000 (GMT) 5, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=me.com; s=4d515a; 5, 39 -- t=1473341451; bh=jfI1d3PZY3oNX9zOkn8E9A56aLUoYuvLwIRDwR8KtWM=; 5, 39 -- h=From:Content-type:Subject:Message-id:Date:To:MIME-version; 5, 39 -- b=lThx7juPsh632sCxYaI/lwfX2tKPqreeWGLpz7ssR/Re1JlTJ+/k2czSH/HDCZWbp 5, 39 -- L1Xm0s+fpMWakC9bmaOaD57EQ1wykwqj6zfVre/NLeBm35PJ7G0+ORAbpTq/NcaRC7 5, 39 -- TizWzQZnlsREfQt81lE5FlwVvEdv6a5+SFISnPT3JMYOi6Wxhjs0Fi0UQgfBs9keNC 5, 39 -- 6xIDopCzWFHfdLvarSsF6mwwf1QprHo/w3W5T2xu7Oj9U91pePtVt2B+Yt1yTLiDUR 5, 39 -- 28lQw9IBHHs2aCKXjC0TCt5qeLjfjFJjnK7Ip4koo8ss635yOy0dODBKpgzSMKkwjb 5, 39 -- PFzu8zPgpau2g== 5, 39 -- Received: from wim-hagens-mbp.wlan.embl.de (unknown [194.94.44.220]) 5, 39 -- by pv33p04im-asmtp002.me.com 5, 39 -- (Oracle Communications Messaging Server 7.0.5.38.0 64bit (built Feb 26 2016)) 5, 39 -- with ESMTPSA id {0OD600DVBTJA4720-at-pv33p04im-asmtp002.me.com} for 5, 39 -- Microscopy-at-microscopy.com; Thu, 08 Sep 2016 13:30:49 +0000 (GMT) 5, 39 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, 5, 39 -- definitions=2016-09-08_07:,, signatures=0 5, 39 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 5, 39 -- clxscore=1015 suspectscore=1 malwarescore=0 phishscore=0 adultscore=0 5, 39 -- bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 5, 39 -- engine=8.0.1-1603290000 definitions=main-1609080196 5, 39 -- From: Wim Hagen {wim.hagen-at-me.com} 5, 39 -- Content-type: text/plain; charset=us-ascii 5, 39 -- Subject: Wind turbines & TEM systems 5, 39 -- Message-id: {8048F8C2-46BF-44CC-81C0-CED344C0843A-at-me.com} 5, 39 -- Date: Thu, 08 Sep 2016 15:30:46 +0200 5, 39 -- To: Microscopy-at-microscopy.com 5, 39 -- MIME-version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 5, 39 -- X-Mailer: Apple Mail (2.3124) 5, 39 -- Content-Transfer-Encoding: 8bit 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u892VNZr004856 ==============================End of - Headers==============================
Prof Rob Parton from Brisbane is one of the people who looked into this. I have no papers at hand at the moment, but I am sure a search will easily reveal his work in this area,
Good luck
Jan Leunissen Aurion
} On 9/09/2016, at 12:09, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: n.i.clarke-at-warwick.ac.uk } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } n.i.clarke-at-warwick.ac.uk as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: n.i.clarke-at-warwick.ac.uk Name: Nick Clarke } } Organization: University of Warwick } } Title-Subject: [Filtered] Immunogold spatial resolution. } } Message: Hello, I was wondering if you could provide me with a good reference for immunogold spatial } resolution i.e. distance from antigen to centre of gold particle. 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==============================Original Headers============================== 9, 34 -- From leunissen-at-aurion.nl Thu Sep 8 21:59:57 2016 9, 34 -- Received: from nm34-vm3.bullet.mail.bf1.yahoo.com (nm34-vm3.bullet.mail.bf1.yahoo.com [72.30.239.75]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u892xqAq012450 9, 34 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2016 21:59:54 -0500 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1473390013; bh=JdqtsnaU52I4pYpU4PXhn9/WJYbwg66+Y+RcEjmxyd0=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=nTGeUmTtsIcniQbz/Dmbd+4oruD6p9zaOD8XWUuK+BMMBJS3PUyNs3uelEm7mPjHRbb7n1da0AbHzLkKFd1njOIUQS3HwDu0A5Cg/8ca4QfEeyQ02BVI8DlL8q8LZMzoUCumyR1rsCGFRWqaowieBqArDkbIezkCvE2EckC9KyxPZjEponJH+Z1eEpZiYFMV8Pb1K2wUe4bYgCqQZUD2H9xYWDqcSs332tCaqEaImfcXL2htDJ6uiO89UEal1kpVJwrKhRpzJAL15ePjqGeQ2ZdjX3kWtW5ceMJLc7Wbl1z3X08vzUfla7oV9GL9uIfcexgQmtQ555Fr13TtPKk1ng== 9, 34 -- Received: from [98.139.170.181] by nm34.bullet.mail.bf1.yahoo.com with NNFMP; 09 Sep 2016 03:00:13 -0000 9, 34 -- Received: from [98.139.213.8] by tm24.bullet.mail.bf1.yahoo.com with NNFMP; 09 Sep 2016 03:00:13 -0000 9, 34 -- Received: from [127.0.0.1] by smtp108.mail.bf1.yahoo.com with NNFMP; 09 Sep 2016 03:00:13 -0000 9, 34 -- X-Yahoo-Newman-Id: 499633.97127.bm-at-smtp108.mail.bf1.yahoo.com 9, 34 -- X-Yahoo-Newman-Property: ymail-3 9, 34 -- X-YMail-OSG: lUXHhDkVM1ljJGExZ0Cbdmnohawa8KttyK26.At7rhehZVI 9, 34 -- r7m6PwgE2pqQgL6cdvoTYxXQcubRBz.2PVaK0PKFXhos1PR8rOSL8NKDgpZj 9, 34 -- Ql2L0ZLnVX4YISLSKeWPaqdt.65qXtPj40PHkgSVpOfUlEDMkGs.OFlkbaiS 9, 34 -- NxF6q.STav1AhdqEgg2gGVDESg9dyJfkN8ko307blKC6Wiez_CMOFNZRXjGi 9, 34 -- 0m4236SrP8eE424z6N2pzGBtXG1M5NsCIQTXb1AgvkVMWcoHbxMj84wODOfm 9, 34 -- s6VpSu9GQqAMgWyFvhmhAgoxYrmi7XB4_kpr3Sdil8fIQEZlVX_86h7x4onz 9, 34 -- 6Jz5Uw37DRiMIOFiTHLZLSvqEN91HwOBhMZAIUoubiId.xqB3bkyqaCjjnwG 9, 34 -- eVXuzp8kljUt_sMrghbK1z9JsdpjRriEKgTaGyIlWXQA3AoVwcOdG5nezUSe 9, 34 -- 258uEBxQjsrrvCpxmsMCpZr5O1EkUUCAgbfxRnEuczLJvdlsFw7G8VUvOJob 9, 34 -- nKP6sp8ekWkgqut_d9unUc1ILwMKGuNXGgx_ghISXbsAH6g5TKaKGyLnIpBe 9, 34 -- dKTfmO3tq 9, 34 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 9, 34 -- Content-Type: text/plain; charset=us-ascii 9, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 9, 34 -- Subject: Re: [Microscopy] via WWW: Immunogold spatial resolution 9, 34 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 34 -- In-Reply-To: {201609090009.u8909uG1019533-at-ns.microscopy.com} 9, 34 -- Date: Fri, 9 Sep 2016 15:00:08 +1200 9, 34 -- Message-Id: {665BFA71-A0B6-48BE-9D3F-A9945AC2D9DD-at-aurion.nl} 9, 34 -- References: {201609090009.u8909uG1019533-at-ns.microscopy.com} 9, 34 -- To: microscopy-at-microscopy.com 9, 34 -- X-Mailer: Apple Mail (2.3124) 9, 34 -- Content-Transfer-Encoding: 8bit 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u892xqAq012450 ==============================End of - Headers==============================
I investigated the fine structure of NMJ's in the mouse and would propose: perfuse the animal by standard procedure and dissect _before _osmification step. Try either:
(a) m. sternocostalis (transversus thoracis muscle) on the inner side of the rib cage, not easy to dissect since only 2-4 layers of muscle fibers thick and extremely delicate, however all nmj's are positioned in one "line" and once you know where to look, you have a high number of NMJ's in one section. Pin down the flattened muscle in a Sylgard dish with fine insect needles and osmicate. Flat-embed the specimen and cut out target region as a rectangular strip under binocular and polymerize on an empty resin block.
(b) dissect the thin strip of intercostal muscles ("rib muscles") between two ribs, NMJ's are typically located in the "mid-line" between the two ribs
(c) dissect the muscle part of the diaphragm ( in this case don't hurt the diaphragm during perfusion), wrap it up into a "roll", most nmj's are more or less in the mid of the length of the muscle fibers, and osmicate
(d) if a-c fails, dissect the tongue, osmicate, embed, cut and look in the EM for nerves resp axons (you can spot them beforehand on a semithin section). if you see a small nerve or individual (myelinated) axons, then usually a NMJ is not far away, it takes however some time to search ....
- in general: on a good Richadson-blue-stained semi-thin section you can identify the NMJ's easily with a x63 objective - always first check on a parallel specimen location of NMJ's by acetylcholine-esterase (ACHE) staining, takes 20 minutes at most (ACHE works good on formaldehyde fixed tissue too). ACHE staining is robust, stable and uncritical and MUST work, just take care that you store the acetylthiocholinejodide dry and at -20 Celsius ....
good luck !
Peter
+++++++++++++++++++++++++++++++++
Email: marie.cantino-at-gmail.com Name: Marie Cantino } Organization: University of Connecticut } } Title-Subject: [Filtered] TEM of mouse neuromuscular junction } } Message: I have been asked to help a student who would like to do TEM of mouse neuromuscular } junctions (NMJ). Ive done plenty of TEM on muscle, but never looking specifically for NMJ. This } strikes me as a bit of a needle in a haystack type of effort, since there is generally only one, or } possibly a few per fiber, depending on the fiber type. Does anyone have experience with this? I } see some studies use cholinesterase staining to find the NMJ first at the light level. Is this } technique tricky? Are there other tricks i should know about? Will this require hundreds of } hours of microtomy? Thanks for any help or advice you can offer. } } } Login Host: 137.99.47.108 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------------
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There are few things you can try to distinguish between exposed and slightly-buried Cu conductors:
1. Based on your mentioning of "atomic mass of Sn is very close to the one of Cu" I am suspecting you may be using backscattered electrons for imaging - try secondary electron detector imaging at lowest acceleration you can afford without loosing too much resolution.
2. Apply negative bias to the sample (retarding field) to reduce landing energy of primary electrons. This could work or not, depending on geometry of your instrument, stage, and your instrumentation skills/capabilities.
3. Electroless nickel plating chemicals cost less then Au plating kits, though either one is safe enough to do in ordinary fume hood with gloves and common sense.
4. Exposure to iodine vapor in humid atmosphere (room air) would corrode exposed Cu fairly quickly, making it look "fluffy" or "rough," while buried conductors will remain intact for a while. The tricky part would be to image/scan the sample fairly quickly - once started the corrosion would eventually consume all the Cu layers. You want to image exposed layer once it has been just so slightly corroded on the surface, and immediately polish it away to prevent damage for the underlying layers.
5. Either sulfuric or nitric acid with H2O2 should etch Cu crystallographically, making exposed surface look differently then metal protected by dielectric. You would want to experiment with "disposable" devices and develop reliable procedure prior to immersing "real" one. Same is true for plating, by the way.
6. Image the chip, etch away Cu with FeCl3, wash-rinse, and the image same area again. Buried under dielectric Cu would be on both images, while exposed would disappear after etching. You would need to develop procedure and control time/temperature/concentration of etchant to get repeatable results.
7. Do forward-scattered imaging, or even regular Se imaging at glancing angle - it would be much more sensitive to presence of thin dielectric layer. Foreshortening correction would be required....
Happy Imaging :)
Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/7/2016 2:24 PM, dev.c0debabe-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } we are using a older model SEM to image samples with copper structures } on the surface and just a few nm below the surface at the same time } (typically buried under a thin layer of glass). } Since the copper layer below the surface is just covered by this very } thin glass layer, even with lower acceleration voltages it is very hard } to distinguish on the images whether the imaged copper structures are } located on the surface or below it. } As our goal would be that the copper on the surface clearly looks } different from the copper buried under the thin glass layer, we were } wondering whether we could use a staining procedure. } } For instance, we are aware of a wet chemical procedure allowing to } tin-plate copper. However, without trying it we don't know if it would } result in a considerable different contrast in the SEM as the atomic } mass of Sn is very close to the one of Cu. } Wet chemical gold plating on the other hand involves not only expensive } but also highly toxic chemicals. } } Are you aware of (wet chemical) staining procedures for copper that } produce a considerable contrast and are preferably inexpensive as well } as non highly toxic ? } } Is there maybe another technique we could use ? } } Kind regards, } Stefan } } ==============================Original Headers============================== } 6, 20 -- From dev.c0debabe-at-gmail.com Wed Sep 7 12:07:22 2016 } 6, 20 -- Received: from mail.tbmn.org (mail.tbmn.org [87.118.84.39]) } 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u87H7KSV020041 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Sep 2016 12:07:20 -0500 } 6, 20 -- Received: from [192.168.1.5] (80-108-8-29.cable.dynamic.surfer.at [80.108.8.29]) } 6, 20 -- (using TLSv1 with cipher DHE-RSA-AES128-SHA (128/128 bits)) } 6, 20 -- (No client certificate requested) } 6, 20 -- (Authenticated sender: mne-at-tbmn.org) } 6, 20 -- by mail.tbmn.org (Postfix) with ESMTPSA id 59751276C262 } 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Sep 2016 19:07:35 +0200 (CEST) } 6, 20 -- To: Microscopy-at-microscopy.com } 6, 20 -- From: Stefan Schoenleitner {dev.c0debabe-at-gmail.com} } 6, 20 -- Subject: Staining of Cu for SEM imaging } 6, 20 -- Message-ID: {0aaaa747-f2e7-6478-f22b-ad672dc237c4-at-gmail.com} } 6, 20 -- Date: Wed, 7 Sep 2016 19:07:34 +0200 } 6, 20 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 } 6, 20 -- Thunderbird/45.2.0 } 6, 20 -- MIME-Version: 1.0 } 6, 20 -- Content-Type: text/plain; charset=utf-8 } 6, 20 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course October 11-13, 2016. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further TEM training details and registration information, please follow the link below:
The Pacific Northwest Microscopy Society will hold its 2016 Fall symposium on Thursday November 3rd, continuing Friday November 4th. The program will showcase innovative area scientists pioneering the latest technologies in microscopy. Both invited and contributed talks will be presented at the Portland Shriners Hospital for Children. Workshops will be conducted at the nearby Center for Spatial Systems Biology at the new Oregon Health Sciences University waterfront campus. Here is an opportunity to interact with those at the forefront of emerging technologies in a friendly atmosphere with many opportunities for conversation and collaboration. The preliminary program and registration can be found on the Pacific Northwest Microscopy Society webpage at:
http://pacificnwmicroscopy.org
Please plan to attend this informative program and meet with your local coleagues and vendors.
Thank you,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3101 SW Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Mobile: 503-819-3600
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Email: jdsugar-at-sandia.gov Name: Josh Sugar
Organization: Sandia National Labs
Title-Subject: [Filtered] Microscopy Technologist Positions at Sandia Livermore
Message: Sandia National Labs in Livermore, CA is looking for experienced and entry level microscopy technologists. Please apply at the links below.
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You are correct that negative biasing of the sample would decelerate primary electrons thus reducing landing energy and penetration depth, but it would take much higher then the 10V - more like a few kV of negative bias.
But here is another bias idea which may work with potentials in 10V range. When you collecting images from areas of Cu covered by dielectric, not only the primary electron beam penetrates the glass, but also secondary electrons must penetrate the same layer of glass to escape. There is electron beam induced conductivity that helps them, but... if you apply small *positive* bias to your sample, it would hold down secondary electrons. Dielectric layer, however thin, would act as additional filter for the secondaries coming from Cu covered by the glass. It is very likely that you would find "sweet spot" where combination of positive bias and attenuation from thin layer of glass would made covered areas of Cu visibly darker then the open areas.....
Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/13/2016 5:00 AM, Stefan Schoenleitner wrote: } Dear Valery, } } first of all I would like to thank you for your extensive list of tips. } This is definitely encouraging and provides me with a bunch of } techniques I could try and evaluate. } } On 09/11/2016 07:45 AM, Valery Ray wrote: } } Hi Stefan, } } } } There are few things you can try to distinguish between exposed and } } slightly-buried Cu conductors: } } } } 1. Based on your mentioning of "atomic mass of Sn is very close to the } } one of Cu" I am suspecting you may be using backscattered electrons for } } imaging - try secondary electron detector imaging at lowest } } acceleration you can afford without loosing too much resolution. } } That was my error. I looked at the periodic table and looked up "Zn" } instead of "Sn" ;) } Usually I like to use the BSE since it allows me to differentiate } between different materials. However, as our SEM is rather old, the BSE } detector is rather insensitive so that I can't go below 10kV. } Yet, even at 5kV the electron beam penetration depth for SiO2 is roughly } 300nm while my glass layers are probably around 100nm. } As a result, even with the SE detector, I can see through. } In fact, this is not a problem as long as I can see the difference of } exposed copper on the surface and buried copper below the surface. } } } 2. Apply negative bias to the sample (retarding field) to reduce landing } } energy of primary electrons. This could work or not, depending on } } geometry of your instrument, stage, and your instrumentation } } skills/capabilities. } } I have an electrical feed through. So the idea would be to bias the } sample with a negative voltage in relation to the GND of the SEM so that } the electrons from the beam are effectively decelerated, right ? } What kind of voltages should I try for that ? Is a couple of volts } ( {10V) sufficient or does it typically have to be more ? } } } 3. Electroless nickel plating chemicals cost less then Au plating kits, } } though either one is safe enough to do in ordinary fume hood with gloves } } and common sense. } } What are the advantages and disadvantages of different plating metals ? } Apparently the options are at least tin-plating, nickel-plating and } Au-plating ? } So my naive approach would be to choose a metal with an atomic mass as } far away from Cu as possible. } I suppose the difference is the thickness of the plating which might } indirectly have an influence on the resolution meaning that copper } structures with a small diameter (i.e. 100nm) would probably either be } not coated or coated with excessive amounts of material ? } } } } 4. Exposure to iodine vapor in humid atmosphere (room air) would corrode } } exposed Cu fairly quickly, making it look "fluffy" or "rough," while } } buried conductors will remain intact for a while. The tricky part would } } be to image/scan the sample fairly quickly - once started the corrosion } } would eventually consume all the Cu layers. You want to image exposed } } layer once it has been just so slightly corroded on the surface, and } } immediately polish it away to prevent damage for the underlying layers. } } Do you have an example SEM image how that would look like ? } Wouldn't the etching process stop rather quickly as soon as the sample } is no longer exposed to the vapor ? Could it be rinsed ? } I think I would prefer a wet chemical approach if possible. } } } 5. Either sulfuric or nitric acid with H2O2 should etch Cu } } crystallographically, making exposed surface look differently then metal } } protected by dielectric. You would want to experiment with "disposable" } } devices and develop reliable procedure prior to immersing "real" one. } } Same is true for plating, by the way. } } Sure. } } } 6. Image the chip, etch away Cu with FeCl3, wash-rinse, and the image } } same area again. } } Is there a reason for using FeCl3 ? So far I performed copper etches } either with HCl+H2O2 or with low HNO3 concentrations. } } } Buried under dielectric Cu would be on both images, } } while exposed would disappear after etching. You would need to develop } } procedure and control time/temperature/concentration of etchant to get } } repeatable results. } } OK, that would be a differential approach where we take image two times, } align the images and create a difference image. Could work ;) } } } } 7. Do forward-scattered imaging, or even regular Se imaging at glancing } } angle - it would be much more sensitive to presence of thin dielectric } } layer. Foreshortening correction would be required.... } } I will try imaging at an angle. Sounds interesting ! } } } Happy Imaging :) } } Thank you so much ! } } Stefan } } } } } Valery } } } } } } Valery Ray } } www.linkedin.com/in/valeryray } } ============================== } } PBS&T, MEO Engineering Company } } 290 Broadway, Suite 298 } } Methuen, MA 01844, USA } } Phone: +1-978-305-0479 - leave a message } } Mobie: +1-978-305-0479 - leave a message } } E-mail: vray-at-partbeamsystech.com } } Web: www.partbeamsystech.com } } Web: www.freudlabs.com } }
==============================Original Headers============================== 6, 39 -- From vray-at-partbeamsystech.com Tue Sep 13 11:18:56 2016 6, 39 -- Received: from nm44-vm9.bullet.mail.gq1.yahoo.com (nm44-vm9.bullet.mail.gq1.yahoo.com [67.195.87.214]) 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u8DGIuFo017610 6, 39 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2016 11:18:56 -0500 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1473783573; bh=lqiOFfTIDPC8aoqwl64SoPK5AAId0j70VSTMSMF/zF8=; h=From:Subject:To:References:Cc:Date:In-Reply-To:From:Subject; b=lZuJ7qOvIb0s6NejfBoydVBe2qIJN8AVi0UETb2yUBXNXNUHxuUxgRwPKSH4f2d9DlRevG27t6v+muJgxIxEWRN5nXocYb0GqIzsb1AngE+pkKDuWOQsQoFwCXts9GIuqIf5EoJHr7hrfXJSRAuXHXW0vTZ0OT2TVd1lbgoL0VBLVe8SviY0DUaQn/wnIhUIbeHhEK+W4SFsUh2wj1sYavWFuZR+Qa9ulJpe1M7NlR0FLbue/yKb/UuIiYiGo5bILgiHJlFD0oFZUAhmbzL9cpVcj5hBd/uLulpUzxEGKHtXFV+B7uefytUJ7tbNIDw8mX1Y69R4xKkJSCIp/eUNfA== 6, 39 -- Received: from [127.0.0.1] by nm44.bullet.mail.gq1.yahoo.com with NNFMP; 13 Sep 2016 16:19:33 -0000 6, 39 -- Received: from [98.137.12.188] by nm44.bullet.mail.gq1.yahoo.com with NNFMP; 13 Sep 2016 16:16:42 -0000 6, 39 -- Received: from [66.196.81.174] by tm9.bullet.mail.gq1.yahoo.com with NNFMP; 13 Sep 2016 16:16:41 -0000 6, 39 -- Received: from [98.139.211.204] by tm20.bullet.mail.bf1.yahoo.com with NNFMP; 13 Sep 2016 16:16:41 -0000 6, 39 -- Received: from [127.0.0.1] by smtp213.mail.bf1.yahoo.com with NNFMP; 13 Sep 2016 16:16:41 -0000 6, 39 -- X-Yahoo-Newman-Id: 674118.54205.bm-at-smtp213.mail.bf1.yahoo.com 6, 39 -- X-Yahoo-Newman-Property: ymail-4 6, 39 -- X-YMail-OSG: 1sgeT7QVM1mQT4Vi82WatehYJbMa5rhc2jTJaMiE.ioBhhN 6, 39 -- ElWbxH1GxY1mggCL8EOIBYj54deg3AKcyUC87CR2xLVAPnztiglcItnmk3_H 6, 39 -- s90TRVTwLDMUGTH.8n_CluvRtPuHLakiDci0F0zvr8gEdHPMuLqvAF7Urrpt 6, 39 -- DAYdVaQxVjbrHohp69zs4_R5m.ROy9csZoKqZtjf4TFzVTKRXTJbCaKWxHQx 6, 39 -- 8lGN6pV7uGpdoP0PrrLK5Iz.90X2XDbb3W_DHP.EagjGdgyTO4bLYhkdPPMm 6, 39 -- lKSNeFCYtErQXpuVstsotn_PtBTSetrmfKaNGgXS7JjPEMeeo096.hx6cM9u 6, 39 -- AytGhvqfW2b7WVzx2L43KVclbLgQEWb5Io2ovFvQqAVAKJbTPMgV_FvIpacK 6, 39 -- DYdX5KXxMh2QIeb8eUZvMC2RPC4Y3iKYEGUCL6riP1ewi5irWntgvpbOAW3y 6, 39 -- 0lwvI56jJ072kiKcBdbsyvbt6F9Q3QKUOBCzPoWL5C0SjqvJ2haILcoUpjt2 6, 39 -- JPFWP74RryLxP0Hx4jtc0h4UNaUBMkTNedilgDYYjSKtJWQrxo7ptD.UkxDX 6, 39 -- 9VImarbhJoQqYMj.n65kV 6, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 39 -- From: Valery Ray {vray-at-partbeamsystech.com} 6, 39 -- Subject: Re: [Microscopy] Staining of Cu for SEM imaging 6, 39 -- To: Stefan Schoenleitner {dev.c0debabe-at-gmail.com} 6, 39 -- References: {201609071824.u87IOCcM024857-at-ns.microscopy.com} 6, 39 -- {2d911adf-b0bb-ea18-53ff-7d72aed78e03-at-partbeamsystech.com} 6, 39 -- {c1aeb1dd-29e3-1305-455d-bf35878cc796-at-gmail.com} 6, 39 -- Cc: microscopy-at-microscopy.com 6, 39 -- Message-ID: {d0c06ec9-913a-c3c1-0ad1-a48d3346c044-at-partbeamsystech.com} 6, 39 -- Date: Tue, 13 Sep 2016 12:16:37 -0400 6, 39 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 6, 39 -- Thunderbird/45.2.0 6, 39 -- MIME-Version: 1.0 6, 39 -- In-Reply-To: {c1aeb1dd-29e3-1305-455d-bf35878cc796-at-gmail.com} 6, 39 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jdsugar-at-sandia.gov Name: Josh Sugar
Organization: Sandia National Labs
Title-Subject: [Filtered] Microscopy Technologist Positions at Sandia Livermore
Message: (Apologies to the microscopy community, previous post had bad links, these should be better.) Sandia National Labs is looking for experienced and entry level microscopy technologists. Please see the job using the links below or searching for the Job ID in the Sandia careers website. Please do not email me directly, applications must be submitted through the website to be considered.
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Title-Subject: [Filtered] Electron Microscopy Sciences sponsors Fall 2016 Workshop
Message: Aurion and Electron Microscopy Sciences are pleased to announce they will hold another of their immensely popular workshops on ImmunoGold Silver Staining at the University of Maryland, Baltimore, Core Imaging Facility, from October 19-21, 2016. The course objectives are to provide researchers the opportunity to learn the theory and practice of immunogold labeling and to promote technology exchange and research collaboration by allowing participants to process their own samples under expert guidance. The ImmunoGold Silver Staining Workshop is designed and taught by Mr. Peter van de Plas who has been with Aurion since 1991. Mr. van de Plas worked closely together with Dr. Leunissen in founding a firm basis for Aurion including development of product applications. He has been invited to many international microscopy conferences and workshops and is especially experienced in providing hands-on training. The Electron Microscopy Core Imaging Facility (EMCIF) provides electron microscopy related research and imaging services to all faculty and staff of the University of Maryland campus and the academic and industrial community in the Washington D.C. and Baltimore areas. The three day course will cover the properties of gold particles and their protein conjugates, theories underlying immunogold labeling protocols, and silver enhancement of gold particles. Also covered in the course will be immunogold labeling on a variety of sample preparations for LM, immunogold labeling for EM, pre/post-embedding double immunogold labeling, and background minimization in immunogold labeling. Please visit the course webpage for additional information, as well as to register and pay online, at www.emsdiasum.com/microscopy/products/immunogold/workshop.aspx. Or download our brochure at www.emsdiasum.com/workshop. Registration is limited to 16 participants. Event contact: Stacie Kirsch, stacie-at-ems-secure.com, 800-523-5874.
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Email: lpq023-at-mail.com Name: PQ
Organization: -
Title-Subject: [Filtered] Cryo TEM - Blotting with filter paper
Message: Dear all,
I have a question with regards to cryo TEM sample preparation.
I was wondering, what will be the effect of different filter papers used for blotting the samples? From what I know, the biggest difference between filter papers is their particle retention. Is this a critical criteria when it comes to blotting the samples?
Also, most papers report I come across usually report using Whatman Grade #1 filter papers to do the blotting. Currently, I only have access to Advantech Grade #1 filter papers. Will this affect the result of my blotting and sample preparation?
I am a beginner in terms of the cryo TEM method and would really like to work on my technique to better my cryo TEM sample preparation skills. Any feedback/comments/suggestions/advice would be greatly appreciated.
Thank you in advance!
Sincerely, PQ
P.S. I am not sure if it could be relevant, but just in case, I am using a Gatan cryoplunge for my sample preparation.
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Hello all! Our facility has a secondhand Zeiss EM109 TEM, which we've recently had serviced. Part of this servicing included getting the coils adjusted mechanically, and it should be in peak condition. However, I have a lot of difficulty getting things into clear focus. I've gotten (I think) quite experienced with aligning and stigmating it, but that doesn't seem to fix things -- indeed, after capturing a blurry image and realigning, the next image of the same sample often looks worse. I suspect that the stigmation is off, but I'm not sure; often I can get better results by systematically capturing images at different focus settings (though of course even with a digital camera this takes a long time, and doesn't do the specimen any favours). Are there any tricks to this?
One question that I have in particular concerns the projector stigmation. I set this using the caustic, which I try to get to look like a Mercedes-Benz symbol (a perfectly symmetrical three-pointed star in a circle). However, changing the low-mag focus (the taller inner knob that doesn't click) changes the appearance of the caustic, such that at one focus setting (say, with the caustic spread out) the caustic is symmetrical, and at another (in this case when it's smaller) it isn't. Is there a "sweet spot" that I should have that focus set to (i.e., should I make the caustic as small as possible while still visible, or as large)?
I should note that I have experimented a little with the stigmation while viewing specimens, and haven't noticed any effect (unlike my experience with a Tecnai-12, on which I could correct the astigmatism just looking at the image). But I do suspect there is a stigmation problem somewhere in the scope, and just need to know how to fix it.
Any advice on the care and feeding of this 'scope would be much appreciated.
Thanks! Aaron
Aaron A. Heiss, Ph.D. Research Scientist and Simons Foundation Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
What are the solutions you have to record videos on the microscope? I'm using a Tecnai F20 (S)TEM with Gatan DM1.83.842, slow scan CCD 1kx1k 794. I'm open to all sorts of ways to capture videos during the observation, either by software to capture the screen display, or by DM scripts. All the best, Zhou
Dr Z Zhou Research Fellow Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
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It is very many years since I used a Zeiss 109, so my memory of its layout is long gone, but I may be able to help you? The image in any TEM is created "in focus" by interaction between the objective lens and the lens which immediately follows it. Any problem with the clarity of this image will be related to the objective lens setting and the setting of the objective lens stigmators. Problems in any other lens (other than instabilities) will not have any influence on the image sharpness. You say that the instrument has been serviced by a person who knows the 109, so we must expect it to be free of high voltage or lens problems. That brings us back to the objective lens, but most of all in your case, the objective astigmatism. You note that with a through focal series the image improves, this goes hand in hand with astigmatism. At true focus the astigmatism will be minimised but as you move out of focus it will destroy the image quality. Please look into the objective stigmator, it may be a double strength system like all modern instruments, or it may be in the style of early instruments. Early instruments used a stigmator which provided a device to change the stigmator field strength, combined with the ability to change the direction of that field. This system was called strength and rotate and it may be the style on the 109. From the point of best focus change the strength control until you see it change the image. Then change the rotate control until you find the point that improves the image quality. Then back off the strength slightly, check the rotation, check the focus. Repeat this procedure until the image is satisfactory.
Best of luck with your problem.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: aheiss-at-amnh.org [mailto:aheiss-at-amnh.org] Sent: 14 September 2016 15:01 To: protrain-at-emcourses.com
Hello all! Our facility has a secondhand Zeiss EM109 TEM, which we've recently had serviced. Part of this servicing included getting the coils adjusted mechanically, and it should be in peak condition. However, I have a lot of difficulty getting things into clear focus. I've gotten (I think) quite experienced with aligning and stigmating it, but that doesn't seem to fix things -- indeed, after capturing a blurry image and realigning, the next image of the same sample often looks worse. I suspect that the stigmation is off, but I'm not sure; often I can get better results by systematically capturing images at different focus settings (though of course even with a digital camera this takes a long time, and doesn't do the specimen any favours). Are there any tricks to this?
One question that I have in particular concerns the projector stigmation. I set this using the caustic, which I try to get to look like a Mercedes-Benz symbol (a perfectly symmetrical three-pointed star in a circle). However, changing the low-mag focus (the taller inner knob that doesn't click) changes the appearance of the caustic, such that at one focus setting (say, with the caustic spread out) the caustic is symmetrical, and at another (in this case when it's smaller) it isn't. Is there a "sweet spot" that I should have that focus set to (i.e., should I make the caustic as small as possible while still visible, or as large)?
I should note that I have experimented a little with the stigmation while viewing specimens, and haven't noticed any effect (unlike my experience with a Tecnai-12, on which I could correct the astigmatism just looking at the image). But I do suspect there is a stigmation problem somewhere in the scope, and just need to know how to fix it.
Any advice on the care and feeding of this 'scope would be much appreciated.
Thanks! Aaron
Aaron A. Heiss, Ph.D. Research Scientist and Simons Foundation Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
To answer your question more precisely it would be interesting to know at {which magnification level [ZEISS TEM109 ....the old analog= I,II,III+) or still a recently digitalized version? } you have the focusing problems. This might be combined also with your problem concerning the "caustic figure" : ["However, changing the low-mag focus (the taller inner knob that doesn't click) changes the appearance of the caustic ++), such that at one focus setting (say, with the caustic spread out) the caustic is symmetrical, and at another (in this case when it's smaller) it isn't.]
+) I ="low-mag level": Range x 150 - x 1100; II = "middle-mag level" = x 3,000 - x 20,000; III = "High mag level"= x 30,000 - x250,000 and (HM) = x 400,000 ++) that's right.... but focusing at the middle-, and high- mag-level as compared to focusing at the low-mag level is another { { procedure/another "knob" } } if I remember correctly....
Normally, if "my" TEM would have been serviced (as yours) and I can't get easily {micrographs in focus} at any mag-level - I am sure I would have { seriously invited again } the service man coming in again to have a look into the machine and poti's (potentiometers) again (not knowing whether your 109 is in classical (analog) or a modernized fashion. It would also be interesting IF you own a - and if yes, WHICH - Operating Manual you have to reread the focusing procedure which for the low-mag-level is different from the middle-mag-level). If you could upload some images of the "knobs" etc. it would be of benefit in order to help you... (admitting that I have worked with a ZEISS-TEM109 vintage 1978 until 2015 without any focusing problem you described).
Regards and best wishes, Wolfgang MUSS PhD. Emeritus member of MSA SALZBURG, Austria
============================================================================ ========= -----Ursprngliche Nachricht----- Von: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Gesendet: Donnerstag, 15. September 2016 11:08 An: wij.muss-at-aon.at Betreff: [Microscopy] RE: Help with Focussing a Zeiss EM109 ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Aaron
It is very many years since I used a Zeiss 109, so my memory of its layout is long gone, but I may be able to help you? The image in any TEM is created "in focus" by interaction between the objective lens and the lens which immediately follows it. Any problem with the clarity of this image will be related to the objective lens setting and the setting of the objective lens stigmators. Problems in any other lens (other than instabilities) will not have any influence on the image sharpness.
You say that the instrument has been serviced by a person who knows the 109, so we must expect it to be free of high voltage or lens problems. That brings us back to the objective lens, but most of all in your case, the objective astigmatism. You note that with a through focal series the image improves, this goes hand in hand with astigmatism. At true focus the astigmatism will be minimised but as you move out of focus it will destroy the image quality.
Please look into the objective stigmator, it may be a double strength system like all modern instruments, or it may be in the style of early instruments. Early instruments used a stigmator which provided a device to change the stigmator field strength, combined with the ability to change the direction of that field. This system was called strength and rotate and it may be the style on the 109. From the point of best focus change the strength control until you see it change the image. Then change the rotate control until you find the point that improves the image quality. Then back off the strength slightly, check the rotation, check the focus. Repeat this procedure until the image is satisfactory.
Best of luck with your problem.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: aheiss-at-amnh.org [mailto:aheiss-at-amnh.org] Sent: 14 September 2016 15:01 To: protrain-at-emcourses.com
One question that I have in particular concerns the projector stigmation. I set this using the caustic, which I try to get to look like a Mercedes-Benz symbol (a perfectly symmetrical three-pointed star in a circle). However, changing the low-mag focus (the taller inner knob that doesn't click) changes the appearance of the caustic, such that at one focus setting (say, with the caustic spread out) the caustic is symmetrical, and at another (in this case when it's smaller) it isn't. Is there a "sweet spot" that I should have that focus set to (i.e., should I make the caustic as small as possible while still visible, or as large)?
I should note that I have experimented a little with the stigmation while viewing specimens, and haven't noticed any effect (unlike my experience with a Tecnai-12, on which I could correct the astigmatism just looking at the image). But I do suspect there is a stigmation problem somewhere in the scope, and just need to know how to fix it.
Any advice on the care and feeding of this 'scope would be much appreciated.
Thanks! Aaron
Aaron A. Heiss, Ph.D. Research Scientist and Simons Foundation Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
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Title-Subject: [Filtered] Spring clamp ring - TEM single tilt holder. Message: Hi All, A ring of spring clamp in FEI single tilt holder is coming out very frequently due to one of the arm of spring clamp is loosen. I am afraid, it may fall apart in near future. Unfortunately our TEM is not cover with contract currently. Is there any home remedy to fix this problem ?
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Organization: University of Connecticut, Biosciences Electron Microscopy Laboratory
Title-Subject: [Filtered] section folds
Message: Several people asked me to report on what methods worked best for us, after I posted a question about eliminating section folds. I posted a summary of the responses we got, but had to wait for our microtomy expert to return and answer this question. Here is the response from Dr. Maritza Abril in our lab: "I tested the tips generously provided for the trimming, microtomy, picking up sections from the knife boat and for drying the sections on the glass slide as our samples were already fixed and embedded. I tried individual suggestions as well as several combinations including: cutting thinner (0.5 m) sections; applying solvent vapors (trichloroethylene and chloroform) to sections still in the knife boat by waving a Q-tip over the sections; using untreated slides, trimming away the excess resin; placing a few drops of acetone on a Q-tip on the frosted end of the slide and covering the slide with a Petri dish until the water drop containing the section dried; adding 1% ethylene glycol to the water drop on the slide; decreasing/increasing the temperature (70C/ 90C) or drying at room temperature; and staining at 70C and 90C. These are the techniques that minimized wrinkles. A word of caution, when drying untreated slides at room temperature make sure to reheat after all water and/or solvent is gone to ensure adhesion of the sections. The optimal minimization of wrinkles was obtained by cutting 1 m thick sections, trimming away the empty resin on all sides of the block (as we could not make the block smaller since we were looking at a tobacco graft), and picking up the sections on 10% acetone (instead of distilled water) on Superfrost Plus slides. Sections were dried by putting the slide, section side down, over a small open Petri dish (previously heated) to evaporate on the hotplate. Once dry they were placed directly on the hotplate, section side up, and stained at 70C."
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Organization: Max Planck Florida Institute for Neuroscience
Title-Subject: [Filtered] MPFI EM workshop 2016 - CLEM workflows -
Message: The Electron Microscopy Core Facility from Max Planck Florida Institute for Neuroscience will host this exciting 2-day workshop, providing you with educational lectures by experts in the field and hands-on training that you can adapt to your own projects. Dates: November 2-3, 2016
Workshop Highlights 1. Presentations by specialists reviewing CLEM technologies and applications 2. Casual reception with specialists for Q & A 3. Interactive tour of EM Core facilities and brief demo of the latest equipment 4. Hands-On Session (2nd Day) for ATUMtome (RMC/Boeckeler), Atlas5 AT with Merlin VP Compact & Gemini SEM300 (Carl Zeiss), and others. Registration Deadline; October 13, 2016
Registration fees; First day lectures only, Free Lectures and hands-on demonstration (Limited to 12 seats), $30
Please see details; https://www.maxplanckflorida.org/education/courses/EM-course/EMC-2016/
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The University of Illinois at Urbana-Champaign, Materials Research Laboratory is putting on it’s annual Biological Fall Conference on November 2-3rd. The title this year is “Diverse Fields of Study.. Steps Toward Medicine"
Everyone is invited to come, the registration is only $25 (for food) and it includes a Vendor Fair, Student Competition, Tours and a whole line up of speakers.
We have speakers on hydrogel printing, nano gold research, prosthetic hands that move and can feel pressure, bone implants etc.
Registration, directions to be up shortly, competition rules and more information can be found at: http://mrl.illinois.edu/mrl-biological-conference-2016
The student competition is open to all academia! We also have a division in the competition for post docs.
Simply have a topic that involves something biological, can use some of the same instruments as are found in our core facilities, talk for 15 minutes and present a poster. Those competing will get their registration fee refunded. Winners take home $99 first place, $50 second place and have their image on our website for several months.
Registration for the competition is part of the regular registration for the conference. Upload a half page abstract during the registration. DEADLINE FOR REGISTRATION to compete is OCTOBER 1st.
Hope you can come!
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
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Email: Desertrat99-at-verizon.net Name: Eric Rosen
Organization: UCLA
Title-Subject: [Filtered] Transition from Biological to Material science EM? Message: Hi all, Curious to know how difficult it would be to transition from Bioogical Em to Material Science? I have want d to learn Material science EM for a while but there are no courses offered down here in the LA area I know of.
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Title-Subject: [Filtered] JEOL 840 with JEOL Orion software
Message: We acquired an upgrade (from our T300) that was working when we bought it. We cannot get the software to work though. From what we can tell all connections seem correct. When we click to load the Orion program the main loading screen pops up "Loading imaging system" then the computer completely and only a hard reset gets it going again. Even the keyboard locks up (caps/numbers lock does nothing). We are running Windows XP and version 1.72.08 Any help is greatly appreciated!!
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Title-Subject: [Filtered] Job opening at UC Riverside
Message: The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of California at Riveside has an open position for electron microscopy laboratory technician. Position responsibilities include operation and routine maintenance of electron microscopes, FIB, ancillary equipment, and all phases of sample preparation and analysis of various types of materials and tissue. This position includes assisting and training facility users in specimen preparation, instrument operation, and related techniques.
To apply for the job visit: https://irecruitportal.ucr.edu/irecruit/!Controller?action=jobs_webui.show_page&page=jobs_browser&public=true&module=jobs.
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The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of California at Riveside has an open position for electron microscopy laboratory technician. Position responsibilities include operation and routine maintenance of electron microscopes, FIB, ancillary equipment, and all phases of sample preparation and analysis of various types of materials and tissue. This position includes assisting and training facility users in specimen preparation, instrument operation, and related techniques.
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The job number in the listing is : 201609161802.
//////////////////////////////////////////////////////////////////////////////// Krassimir N. Bozhilov
Central Facility for Advanced Microscopy and Microanalysis - Research and Econ. Development Office Materials Science & Engineering - BCOE University of California Riverside, CA 92521
No laughing, I am curious to know what is the best kind of bottle/container for various EM stuff.
I have always been a glass kind of guy. Thought plastic might have issues with some chemicals and I have had very old plastic bottles get brittle and go to pieces on the shelf.
But, I am willing to reconsider. Plastic is lighter, maybe cheaper, and less breakable while young.
Is plastic OK for most things. I’m thinking about storing stock solutions of buffers, acetone, alcohol, fixatives etc.
I need to buy some new supplies and am wondering which way to go. Not exactly the paper or plastic question at the grocery store, but what are your thoughts?
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
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Email: LindaG-at-adhb.govt.nz Name: Linda Graham
Organization: LabPlus
Title-Subject: [Filtered] NZ Microscopy Conference - 31 Jan - 3 Feb 2017
Message: Notice of Conference on behalf of the Conference Committee.
Registration and abstract submission are now open for this upcoming 2017 conference with more details on the conference website www.microscopy2017.co.nz
There are a number of interesting workshops being held on the day before the conference proper. These workshops have limited numbers so please register early if you are interested in coming to a workshop.
We have pleasure in confirming our invited international speakers listed below;
Professor Julie Cairney, University of Sydney, Materials at the atomic level
Scientia Professor Katharina Gaus, University of New South Wales, Super-resolution and single molecule science
Dr Arun Sampathkumar, Max Planck Institute of Molecular Plant Physiology, Plant cell biology
Dr Lucia Burgio, Department of Conservation Science, Victoria and Albert Museum, Analysis and technical examination of museum objects, with Raman microscopy, X-ray fluorescence and optical microscopy
Dates: Workshops 31st January 2017. Conference: 1-3 February 2017. Venue AUT, Auckland, NZ.
Dr Ian Hallett Conference Convener - Microscopy 2017
Senior Scientist Team Leader - Microscopy and Cell Walls
T: +64 9 925 7027 F: +64 9 925 7001 ian.hallett-at-plantandfood.co.nz www.plantandfood.co.nz The New Zealand Institute for Plant & Food Research Limited
Regards Linda M Graham Electron Microscopy Technical Specialist Anatomical Pathology LabPlus Phone 09 3737 599 (or 9090 from A+) extension 86474 or 86052 Mobile: 021 915025 email: lindag-at-adhb.govt.nz
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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] B field strength in JEOL 2100FX
Message: We are looking for the B field strength in a JEOL 2100.
Do you know someone who might have measured it or have a value that is at least by 10% close to the real value? Thanks
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See CHRISTENSON, K., & EADES, J. (1986). On “parallel” illumination in the transmission electron microscope. Ultramicroscopy, 19(2), 191–194. http://doi.org/10.1016/j.ultramic.2006.04.029
You could do the experiment described in this paper to measure the Larmor frequency. With near-parallel illumination, take a series of images over a large Z range, then align the images in e.g. MIDAS which is part of IMOD software (http://bio3d.colorado.edu/imod/), this will give the image rotation per focus step used, the Larmor frequency.. Once you have the Larmor frequency, you can calculate the field strength in the immersion lens.
This paper from the same authors has a bit more detail: CHRISTENSON, K., & EADES, J. (1988). Skew Thoughts on Parallelism. Ultramicroscopy, 26, 113–132.
Best,
Wim Hagen EMBL Heidelberg
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==============================Original Headers============================== 9, 41 -- From wim.hagen-at-me.com Thu Sep 22 10:47:14 2016 9, 41 -- Received: from pv33p04im-asmtp001.me.com (pv33p04im-asmtp001.me.com [17.143.181.10]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u8MFlE0u017865 9, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Sep 2016 10:47:14 -0500 9, 41 -- Received: from process-dkim-sign-daemon.pv33p04im-asmtp001.me.com by 9, 41 -- pv33p04im-asmtp001.me.com 9, 41 -- (Oracle Communications Messaging Server 7.0.5.38.0 64bit (built Feb 26 2016)) 9, 41 -- id {0ODW00E00WHFLK00-at-pv33p04im-asmtp001.me.com} for Microscopy-at-microscopy.com; 9, 41 -- Thu, 22 Sep 2016 15:48:09 +0000 (GMT) 9, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=me.com; s=4d515a; 9, 41 -- t=1474559289; bh=HgxNNZHIgcafdXkbzCE/hE9RnorHLD5jvwBNdHIYlW0=; 9, 41 -- h=Content-type:MIME-version:Subject:From:Date:Message-id:To; 9, 41 -- b=Sg3ozqC/jfS94OYpuSiwsehxJXIhxK/PBIbuG3JgqUJLpbfLyHXmOjR0btHgOPcnp 9, 41 -- sGyGgnY7yjllu6dowiPc8wP+G1+4XVBm28D75QWWAznXBXvV7zv0Nc+CwToIq5GpmY 9, 41 -- Q3mtRJ/1UHlnhEUU1K+ULU75QOXxtqm8SRsVKqFUpXuvrgfFqwoJWkdS2q6o1hhhJi 9, 41 -- A1+HRN+ODGobaVDwnI3HKLq12tDG3X8UOPgwZH7aVkp9fxfGKHV/e4En+eBqsc5U+Q 9, 41 -- bfV8zoRs40iJjCKddX9IOFkm4o8enag/UKrQI7E4CB7Y6YGDvxDSp5TLmBxbdzpkSA 9, 41 -- rlIXe68qm1Gfg== 9, 41 -- Received: from wim-hagens-mbp.wlan.embl.de (unknown [194.94.44.220]) 9, 41 -- by pv33p04im-asmtp001.me.com 9, 41 -- (Oracle Communications Messaging Server 7.0.5.38.0 64bit (built Feb 26 2016)) 9, 41 -- with ESMTPSA id {0ODW005MTX85N750-at-pv33p04im-asmtp001.me.com} ; Thu, 9, 41 -- 22 Sep 2016 15:48:08 +0000 (GMT) 9, 41 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, 9, 41 -- definitions=2016-09-22_08:,, signatures=0 9, 41 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 9, 41 -- clxscore=1034 suspectscore=2 malwarescore=0 phishscore=0 adultscore=0 9, 41 -- bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 9, 41 -- engine=8.0.1-1603290000 definitions=main-1609220282 9, 41 -- Content-type: text/plain; charset=utf-8 9, 41 -- MIME-version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 9, 41 -- Subject: Re: [Microscopy] viaWWW:B field strength in JEOL 2100FX 9, 41 -- From: Wim Hagen {wim.hagen-at-me.com} 9, 41 -- In-reply-to: {201609221240.u8MCevPU031127-at-ns.microscopy.com} 9, 41 -- Date: Thu, 22 Sep 2016 17:48:04 +0200 9, 41 -- Message-id: {1A05FA36-2CCC-4AA5-8F7F-293407833933-at-me.com} 9, 41 -- References: {201609221240.u8MCevPU031127-at-ns.microscopy.com} 9, 41 -- To: Microscopy-at-microscopy.com, hasan.ali-at-angstrom.uu.se 9, 41 -- X-Mailer: Apple Mail (2.3124) 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u8MFlE0u017865 ==============================End of - Headers==============================
Best Regards Hasan Ali ________________________________________ X-from: Wim Hagen [wim.hagen-at-me.com] Sent: Thursday, September 22, 2016 5:48 PM To: Microscopy-at-microscopy.com; Hasan Ali
Oh Pamela,
Your question raised the hair on the back of my irony bone. I have never chipped a Coplin jar, because I always clean them by hand. Thus, if one shows up with a chip, I assume it is feeling suicidal. I did, once, try to repair a chipped Coplin, but it was insistent and just chipped elsewhere. I can clearly recall that day, oh so long ago, when I finally gave up and ordered a case of the things. There are few items in the armamentum of the histologists lab that can be as ornery as chippy Coplin jars. I can still remember the days when I gave up and went home early.
On the practical side, I can only offer advices that I will not put in print, other than to say, "Don't bump them on anything anymore! They must feel pain or aggravation about the way they are treated in the workplace. Or, it might be that they are upset by failing to have authority over only 10 slides, and often only ONE at a time. It's a wonder that they haven't unionized."
Wishing you luck,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work)
-----Original Message----- X-from: Romundstad, Pamela K via Histonet [mailto:histonet-at-lists.utsouthwestern.edu] Sent: Friday, September 23, 2016 2:32 PM To: histonet-at-lists.utsouthwestern.edu
X-from: joaoanatomia-at-gmail.com
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Email: joaoanatomia-at-gmail.com Name: Joao Paulo Rodrigues Marques
Organization: University of Sao Paulo
Title-Subject: [Filtered] CryoJane Slides - how to attach the sections to slides?
Message: I am trying to attach my sections from Leica Cryostat to the slides using CryoJane Tape Transfer System (1x slides, tapes and UV light). My sections are good but when I stained the slides my sections detaches and this is causing some problems because I need to wash my slides several times (In situ hybridization and immunohistochemistry). What can I do to solve this problem? How keep the sections attached to the slides? Does someone has experience with CryoJane slides can give me more information?
Thanks and regards,
Joao Paulo R. Marques
Louisiana State University University of Sao Paulo
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} On Sep 21, 2016, at 1:43 PM, jkrupp-at-deltacollege.edu wrote: } } No laughing, I am curious to know what is the best kind of bottle/container for various EM stuff. } } I have always been a glass kind of guy. Thought plastic might have issues with some chemicals and I have had very old plastic bottles get brittle and go to pieces on the shelf. } } But, I am willing to reconsider. Plastic is lighter, maybe cheaper, and less breakable while young. } } Is plastic OK for most things. I’m thinking about storing stock solutions of buffers, acetone, alcohol, fixatives etc. } } I need to buy some new supplies and am wondering which way to go. Not exactly the paper or plastic question at the grocery store, but what are your thoughts? } } Jon } } Jonathan Krupp } ASB&T } San Joaquin Delta College } 5151Pacific Ave. } Stockton, CA 95207 } (209) 954-5284 } jkrupp-at-deltacollege.edu
Hi Jon, It is not enough to specify “plastic”. Some plastics are softened or dissolved by acetone, for example. On the other hand, NaOH dissolves glass (over a long period of time), so the container should be selected for each specific reagent. Polyethylene and polypropylene are among the most stable plastics and should hold compatible reagents safely for a very long time, and they are unlikely to be damaged with even the roughest handling students will dish out. OsO4 can be problematic, but I think that polyethylene is OK for it—perhaps someone with more experience than I can correct me if I’m wrong. There are charts that list materials-reagents compatibility, and I’m sure they are available on the web. It’s these simple things that can play a role in maintaining lab safety, and you are to be commended for taking it seriously. Yours, Bill
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We have a fantastic job opportunity for a cryo-TEM specialist to help internal and external users with our Tecnai Polara, Talos Arctica and two Titan Krios microscopes.
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The Department of Physiology and Neurobiology seeks applications from outstanding scientists for a tenure-track position at the assistant, associate, or full professor level. The successful candidate will be expected to maintain an independent research program addressing fundamentally important questions in any area of Physiology and Neurobiology and serve as Faculty Director of the Bioscience Electron Microscopy Laboratory (BEML; emlab.uconn.edu). Applicants must have a Ph.D. or equivalent degree in Physiology, Neurobiology, Biophysics, Cell Biology, Structural Biology or related area; significant post-degree research experience using Electron Microscopy; an outstanding scholarly record including publications in leading journals; deep commitment to effective instruction at the undergraduate and graduate levels; strong communication skills; and the ability to promote diversity through their teaching and research programs. The University of Connecticut (UConn) is in the midst of a transformational period of growth supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/), the $1B Bioscience Connecticut (http://health.uconn.edu/bioscience-ct/) investments, and a bold new Academic Plan: Path to Excellence (https://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1). Cutting-edge STEM research is further supported by the Center for Open Research Resources and Equipment (http://core.uconn.edu/) , which oversees an exceptional collection of research support facilities. We are pleased to continue these investments by inviting applications for this tenure-track faculty position at the rank of Assistant Professor, one of two positions currently open in the Department of Physiology and Neurobiology (http://www.pnb.uconn.edu/).
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Message: Does anyone have a supply of Cupromeronic blue? I have a researcher that wants to use it to enhance proteoglycans in cornea for examination under TEM. I have been unsuccessful in locating a company that supplies it. I will need 0.5g
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Title-Subject: [Filtered] Desktop carbon coater
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} On Sep 26, 2016, at 3:59 AM, MUSS Wolfgang (SCUR Board Member) {wij.muss-at-aon.at} wrote: } } Dear Bill: } (Dear Jon), } } Thanks for your posting on "Best kind of bottles" which -as you stated -naturally always depends on "what" you are intending to "fill and store into.....". } Most of your proposals I can second (e.g. NaOH as base in Polyethylene/Polypropylene bottles , vs. Acids in (Lab)Glass bottles.... but: } } I would like to comment first to you personally regarding OsO4 storage in polypropylene (generally in "Plastic") bottles: } } I have information ( as it also was my personal experience ) that any kind of plastic containers/bottles (polyethylene, polypropylene, polycarbonate, polystyrene/polystyrole, etc.) with regard to OsO4 (even recycled and therefore highly "pure" OsO2-precipitate) should be avoided. } } OsO4 solution/its vapors (also true for the OsO2 powder one might store after Reprocessing/recycling from used solutions) is highly reactive with "plastic" (which you and others for sure have seen as "black precipitate" inside a refrigerator if storage was not properly done. } } Proper storage of OsO4-solution IMHO only can be provided in a "Double-capped acid-flask/bottle (with grinded stopper/cap fits/seats)" made at least from (brown lab)glass as one can find in some glassware companies' catalogues. } } Even if you use such a flask you have to expect outgassing of the OsO4-vapor , so you might protect users as well as yourself in } i) always using sufficient Parafilm- wrapping of both the stopper/sealing plug as well as the grinded outer glass cap, } ii) placing the "Double-capped acid-flask/bottle" in a suitable fume cupboard / exhauster (if you store at room temperature for rapid use or short-dated applications), or better, } ii) placing and freezing the "Double-capped acid-flask/bottle", wrapped by Parafilm in a separate refrigerator (at least at - 25°C - you can store in frozen state much more longer for later use or long-dated applications and just have to warm up to room temperature in a suitable fume cupboard / exhauster, which might take some 20-40 minutes without further stirring/vortexing) } } I am sorry and therefore apologize for lengthiness and Germanisms in my English writing style....If you would like to supplement your comment on OsO4-storage with what I brought to your attention, please feel free to use any of my words. } } Best wishes and regards, } Wolfgang } } PS: any catalogue of plastic producing company/-ies at least in the last 10 years I found to contain valuable data on properties of diverse 'plastic brands' , for example: } Google search for: " resistance of plastics to solvents " (more than 10.000,000 results), the first address being: http://www.plasticsintl.com/plastics_chemical_resistence_chart.html } } Wolfgang MUSS PhD/Dr. phil. } Former Head of EM-Lab Pathology Inst. SALK-LKH and PMU (Paracelsus Medical Univ.) Salzburg } PRIVATIER / private gentleman / Retiree } since 1st of Dec. 2015 } PRIVATE ADRESS: } Dr. phil. (PhD) Wolfgang (and Ingrid) Muß } Ignaz-Rieder-Kai 19/6 } 5020 SALZBURG AUSTRIA/AUTRICHE/Österreich } Mobile Phone: +43(0)676 5 369 456 } e-mail: wij.muss-at-aon.at } MSA-Member (retired) } Board Member of SCUR (until June 2017)[www.scur.org] } Cf.: http://www.researchgate.net/profile/Wolfgang_MUSS } } The Society for Cutaneous Ultrastructure Research SCUR, } The Skin Imaging Society at: www.scur.org } } Member of } FB-ARMY of Electron Microscopists } https://www.facebook.com/groups/2339731127/ } and } FB-Delta College San Jose Electron microsopy } https://www.facebook.com/groups/2339731127/ } } } } -----Ursprüngliche Nachricht----- } Von: wtivol-at-sbcglobal.net [mailto:wtivol-at-sbcglobal.net] } Gesendet: Montag, 26. September 2016 01:35 } An: wij.muss-at-aon.at } Betreff: [Microscopy] Re: Best kind of bottles? } } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Jon, } It is not enough to specify "plastic". Some plastics are softened or dissolved by acetone, for example. } On the other hand, NaOH dissolves glass (over a long period of time), so the container should be selected for each specific reagent. } Polyethylene and polypropylene are among the most stable plastics and should hold compatible reagents safely for a very long time, and they are unlikely to be damaged with even the roughest handling students will dish out. } OsO4 can be problematic, but I think that polyethylene is OK for it—perhaps someone with more experience than I can correct me if I’m wrong. } There are charts that list materials-reagents compatibility, and I’m sure they are available on the web. It’s these simple things that can play a role in maintaining lab safety, and you are to be commended for taking it seriously. } Yours, } Bill } } } } } On Sep 21, 2016, at 1:43 PM, jkrupp-at-deltacollege.edu wrote: } } No laughing, I am curious to know what is the best kind of bottle/container for various EM stuff. } } I have always been a glass kind of guy. Thought plastic might have issues with some chemicals and I have had very old plastic bottles get brittle and go to pieces on the shelf. } } But, I am willing to reconsider. Plastic is lighter, maybe cheaper, and less breakable while young. } } Is plastic OK for most things. I’m thinking about storing stock solutions of buffers, acetone, alcohol, fixatives etc. } } I need to buy some new supplies and am wondering which way to go. Not exactly the paper or plastic question at the grocery store, but what are your thoughts? } } Jon } } } Jonathan Krupp } } ASB&T } } San Joaquin Delta College } } 5151Pacific Ave. } } Stockton, CA 95207 } } (209) 954-5284 } } jkrupp-at-deltacollege.edu
Dear Wolfgang, I second your advice about OsO4. As I think about it, even PE or PP could have plasticizer with double bonds, and Os black ppt could end up in micro-cracks that develop as a result of reaction with OsO4. Yours, Bill
==============================Original Headers============================== 7, 35 -- From wtivol-at-sbcglobal.net Sat Oct 1 21:28:45 2016 7, 35 -- Received: from nm18-vm4.access.bullet.mail.bf1.yahoo.com (nm18-vm4.access.bullet.mail.bf1.yahoo.com [216.109.115.83]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u922Sj6d024266 7, 35 -- for {microscopy-at-microscopy.com} ; Sat, 1 Oct 2016 21:28:45 -0500 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1475375429; bh=g2atfXs9FsNcLznHK1S8j/d2aonZbKZ8qoz80MlXHY8=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=XOQSvNByBo3CfCaK2B1L31Fz3mWqH2/WxOs40NWCyHuaBWN+tvhspcjy2J4XWXxrXhnFqRcqGK5SFWzAtMgUfNaihcTLNpdf57iumaFPvD77GWm+omAO1KBz7+r01of95gtuJDH+weLSGAJgLFnJphmLfym/ahbX1SPv13M85apNGLUr9RXF/iINzEHwXcD45/AI+TXfE2xbV2ySmfxqCfa9DaVvq5u66nl0dtNKIdI2XzrtkPe7lD27gXHu8m099bTBnaoRLC95C03wDTyJotn8lVK2IkM5CaPr1FhXArVnjno9j+iJZqpyhwoU0vRjreUmhwjLWZn3GJf6Lp93XA== 7, 35 -- Received: from [66.196.81.164] by nm18.access.bullet.mail.bf1.yahoo.com with NNFMP; 02 Oct 2016 02:30:29 -0000 7, 35 -- Received: from [98.139.221.250] by tm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 02 Oct 2016 02:30:29 -0000 7, 35 -- Received: from [127.0.0.1] by smtp120.sbc.mail.bf1.yahoo.com with NNFMP; 02 Oct 2016 02:30:29 -0000 7, 35 -- X-Yahoo-Newman-Id: 518401.39533.bm-at-smtp120.sbc.mail.bf1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: njf8_rUVM1nucFxiSvadDQ64cvcHJ89fYUiN4xzR4YXyHNH 7, 35 -- uk_F2PpAVqTcyHcXxWT7cbWbdsJxLC7vk_sfS_ttVCuPcL0aMNe7S9XF5Ai3 7, 35 -- caxNRLCsdJjfnz4A0seFggPTPSgprikTnVx75Wg79R5grjy6KN1N.D9T08fG 7, 35 -- 6TFq.mxlihIFOgIyhd5k8fFh.4mdciUTjsUZxPIqrVPt71c9Da0zF16JJVa4 7, 35 -- O_i40e4x8GQyTyo6TefH6nmGNBpiCCoEgBQTA3VxjBsaT39yD.rxwbyVliRp 7, 35 -- mxzhraOiLvaGpAWuuGmVACN_Oo7ARj_21uNrV9lgC2vbGrGnNj6xMVSamqI7 7, 35 -- 5VmA0P43cCKxe2NeRxiP7xptRSjicXiFlOQPFez2GxfA67Le8BMVRFoqqTwa 7, 35 -- sOPgQlevkoss.JcsXBNwd_RU1RvANCsyd2jFvjtZ9un.rf9t0LO1.CjLFYdG 7, 35 -- _N5v55YTwZBY5VqdteQAV2XkVkdPzDTa.QUobug7K8Mw.M00XDmIbgQNkoc7 7, 35 -- GgBBJgWkZa9r2YrTBPvjK_pmkhrTiLJVMGuH.QJ8ZFJpsYq9UA8vS.TYthaH 7, 35 -- gOkmFQouRHv8- 7, 35 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 7, 35 -- Content-Type: text/plain; charset=utf-8 7, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 7, 35 -- Subject: Re: Re personal [Microscopy] Re: Best kind of bottles? 7, 35 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 7, 35 -- In-Reply-To: {003501d217e5$172ed320$458c7960$-at-aon.at} 7, 35 -- Date: Sat, 1 Oct 2016 19:30:27 -0700 7, 35 -- Message-Id: {F6E47CB5-DF7A-43D3-93B6-2E1A6849BCE4-at-sbcglobal.net} 7, 35 -- References: {003501d217e5$172ed320$458c7960$-at-aon.at} 7, 35 -- To: "MUSS Wolfgang (SCUR Board Member)" {wij.muss-at-aon.at} , 7, 35 -- microscopy-at-microscopy.com 7, 35 -- X-Mailer: Apple Mail (2.3124) 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u922Sj6d024266 ==============================End of - Headers==============================
Greetings, Our Boston- area company is in immediate need of a Tecnai TEM operator to help cover demand on a current project. If you are in the area and interested, feel free to contact me via the email below.
Dear Listers, we have a small problem with the rotary pump serving our Jeol JEM-3010. Given the age of the pump, we are evaluating whether try to fix the problem or buy a new pump. In the latter case, we would prefer to buy a different brand than the original one provided with the TEM. But we're not sure about the compatibility of the pumps when dealing with the control signal used by the TEM electronics to operate the rotary pump. Does anyone have experience with such an issue? Any contribution will be greatly appreciated.
The Pacific Northwest Microscopy Society will hold its 2016 Fall symposium on Thursday November 3rd, continuing Friday November 4th. The program will showcase innovative area scientists pioneering the latest technologies in microscopy. Both invited and contributed talks will be presented at the Portland Shriners Hospital for Children. Workshops will be conducted at the nearby Center for Spatial Systems Biology at the new Oregon Health Sciences University waterfront campus. Here is an opportunity to interact with those at the forefront of emerging technologies in a friendly atmosphere with many opportunities for conversation and collaboration. We encourage you to present your work in the Friday afternoon session! The preliminary program and registration can be found on the Pacific Northwest Microscopy Society webpage at:
http://pacificnwmicroscopy.org
Please plan to attend this informative program and meet with your friends, colleagues and vendors.
Thank you,
Doug
Douglas R. Keene President, Pacific NW Microscopy Society Micro-Imaging Center Shriners Hospital for Children 3101 SW Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Mobile: 503-819-3600
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Hi, we are using JEOL 2100F electron microscope. From past 2-3 days we can see a sudden "flash" of light on flourescent screen along with a faint "click" sound. Rest of tem works as normal. This id more prevalent if I meddle too much with brightness knob. Can anyone suggest a reason for this?
==============================Original Headers============================== 1, 37 -- From amit.welcomes.u-at-gmail.com Tue Oct 4 00:02:43 2016 1, 37 -- Received: from mail-yb0-f194.google.com (mail-yb0-f194.google.com [209.85.213.194]) 1, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9452hxu005158 1, 37 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 00:02:43 -0500 1, 37 -- Received: by mail-yb0-f194.google.com with SMTP id e2so4125709ybi.0 1, 37 -- for {microscopy-at-microscopy.com} ; Mon, 03 Oct 2016 22:04:35 -0700 (PDT) 1, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 37 -- d=gmail.com; s=20120113; 1, 37 -- h=mime-version:from:date:message-id:subject:to; 1, 37 -- bh=yEXnm1BjITlAyvQM+DuWh72HykWAp7D5DdcExpcdwMk=; 1, 37 -- b=GAP+25GEo+tlhIAS68GN316JZjNkf3M/RhrztUmA51BK12mBFaTjqHOC7DSwU3FWoq 1, 37 -- TLktoOXimJxiQYpdCHG3iIZWkz3rwNtzQ4c8oVQQMNmFZxAq94RPDCJbtJe3/KJmKaVQ 1, 37 -- 9i/z3vTRYSFfK/Hje56v53mHB/aIUFfVdMNwoBC6VaNph9kLBuPT3TSfVpHedluQycYZ 1, 37 -- PLjoM6v9IqswIMFhEBSgIaAqLYkKc/Ws9Cswy1Vl4KG5HlyXOc7OmtxTsguubnOexYRB 1, 37 -- kwvcnx3stsEmkfc8A1PYdn8iUGK53YGQyb+x2yGYqJ4oVSSQiAxlPfcQ1hS8HSYQxEoU 1, 37 -- GPcQ== 1, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 37 -- d=1e100.net; s=20130820; 1, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 1, 37 -- bh=yEXnm1BjITlAyvQM+DuWh72HykWAp7D5DdcExpcdwMk=; 1, 37 -- b=F+5r7T6aKvKiMnHYu93UWjOT2LnrvsywaOi+vQ1UXE9t6y0ijqpctSE/pYEpu0EnQh 1, 37 -- RS2Qq9neXD56582oC56l9spcSm7TFQ93Pr180NDwQzdua2lTjyPt61EqYeaaaTPZ11TJ 1, 37 -- 9lgHJTVvVtbkFjWwrv+cq5TJh2LB9o4slHOmzlbZYhWE03DEZBZi9UQpPHu1o3P3vmV7 1, 37 -- q/T67cVT7XN86C3vyA+dUGvQwjFH4/qtvKb3ABLUYdlOtsp4xGa3nGGYgqyhtQ92yG+p 1, 37 -- VYf2Uh4R0P9JJyWbShGzFYqowUPnpou6LRQeRq934HA+3T4ZriSe302YzitoWSU7jkfs 1, 37 -- qNVw== 1, 37 -- X-Gm-Message-State: AA6/9RkrBwxb3+nlhK52i0Ac6kfNHqREvczwvWWLlNS3AybRJBEX5V6by0ozRYva/wAuzarXMK8bTXWwh3/KIA== 1, 37 -- X-Received: by 10.37.32.68 with SMTP id g65mr1222002ybg.190.1475557475283; 1, 37 -- Mon, 03 Oct 2016 22:04:35 -0700 (PDT) 1, 37 -- MIME-Version: 1.0 1, 37 -- Received: by 10.37.203.139 with HTTP; Mon, 3 Oct 2016 22:04:15 -0700 (PDT) 1, 37 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 1, 37 -- Date: Tue, 4 Oct 2016 10:34:15 +0530 1, 37 -- Message-ID: {CA+bx8_wx4DnW7P-d4AEAuty0r191PHd12-yH=8JcZcdNwGfU-w-at-mail.gmail.com} 1, 37 -- Subject: Sudden flashes seen on Fluorescent screen of TEM 1, 37 -- To: microscopy {microscopy-at-microscopy.com} 1, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Hi, I believe you have seen an electric discharge. May be the connection between the screen and the ground is pretty bad. This connection is done through a spring under the screen and the circuit for measuring the current received by the screen.
Nicolas STEPHANT
Universit de Nantes Institut Jean Rouxel Service de microscopie lectronique balayage et microanalyse 2 rue de la Houssinire BP 92208 44322 Nantes cdex 3
Tl : 02 40 37 64 26 Ml : nicolas.stephant-at-univ-nantes.fr Web : http://cnrs-imn.fr
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 04/10/2016 07:16, amit.welcomes.u-at-gmail.com a crit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } we are using JEOL 2100F electron microscope. From past 2-3 days we can } see a sudden "flash" of light on flourescent screen along with a faint } "click" sound. Rest of tem works as normal. This id more prevalent if } I meddle too much with brightness knob. } Can anyone suggest a reason for this? } } ==============================Original Headers============================== } 1, 37 -- From amit.welcomes.u-at-gmail.com Tue Oct 4 00:02:43 2016 } 1, 37 -- Received: from mail-yb0-f194.google.com (mail-yb0-f194.google.com [209.85.213.194]) } 1, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9452hxu005158 } 1, 37 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 00:02:43 -0500 } 1, 37 -- Received: by mail-yb0-f194.google.com with SMTP id e2so4125709ybi.0 } 1, 37 -- for {microscopy-at-microscopy.com} ; Mon, 03 Oct 2016 22:04:35 -0700 (PDT) } 1, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 1, 37 -- d=gmail.com; s=20120113; } 1, 37 -- h=mime-version:from:date:message-id:subject:to; } 1, 37 -- bh=yEXnm1BjITlAyvQM+DuWh72HykWAp7D5DdcExpcdwMk=; } 1, 37 -- b=GAP+25GEo+tlhIAS68GN316JZjNkf3M/RhrztUmA51BK12mBFaTjqHOC7DSwU3FWoq } 1, 37 -- TLktoOXimJxiQYpdCHG3iIZWkz3rwNtzQ4c8oVQQMNmFZxAq94RPDCJbtJe3/KJmKaVQ } 1, 37 -- 9i/z3vTRYSFfK/Hje56v53mHB/aIUFfVdMNwoBC6VaNph9kLBuPT3TSfVpHedluQycYZ } 1, 37 -- PLjoM6v9IqswIMFhEBSgIaAqLYkKc/Ws9Cswy1Vl4KG5HlyXOc7OmtxTsguubnOexYRB } 1, 37 -- kwvcnx3stsEmkfc8A1PYdn8iUGK53YGQyb+x2yGYqJ4oVSSQiAxlPfcQ1hS8HSYQxEoU } 1, 37 -- GPcQ== } 1, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 1, 37 -- d=1e100.net; s=20130820; } 1, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 1, 37 -- bh=yEXnm1BjITlAyvQM+DuWh72HykWAp7D5DdcExpcdwMk=; } 1, 37 -- b=F+5r7T6aKvKiMnHYu93UWjOT2LnrvsywaOi+vQ1UXE9t6y0ijqpctSE/pYEpu0EnQh } 1, 37 -- RS2Qq9neXD56582oC56l9spcSm7TFQ93Pr180NDwQzdua2lTjyPt61EqYeaaaTPZ11TJ } 1, 37 -- 9lgHJTVvVtbkFjWwrv+cq5TJh2LB9o4slHOmzlbZYhWE03DEZBZi9UQpPHu1o3P3vmV7 } 1, 37 -- q/T67cVT7XN86C3vyA+dUGvQwjFH4/qtvKb3ABLUYdlOtsp4xGa3nGGYgqyhtQ92yG+p } 1, 37 -- VYf2Uh4R0P9JJyWbShGzFYqowUPnpou6LRQeRq934HA+3T4ZriSe302YzitoWSU7jkfs } 1, 37 -- qNVw== } 1, 37 -- X-Gm-Message-State: AA6/9RkrBwxb3+nlhK52i0Ac6kfNHqREvczwvWWLlNS3AybRJBEX5V6by0ozRYva/wAuzarXMK8bTXWwh3/KIA== } 1, 37 -- X-Received: by 10.37.32.68 with SMTP id g65mr1222002ybg.190.1475557475283; } 1, 37 -- Mon, 03 Oct 2016 22:04:35 -0700 (PDT) } 1, 37 -- MIME-Version: 1.0 } 1, 37 -- Received: by 10.37.203.139 with HTTP; Mon, 3 Oct 2016 22:04:15 -0700 (PDT) } 1, 37 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} } 1, 37 -- Date: Tue, 4 Oct 2016 10:34:15 +0530 } 1, 37 -- Message-ID: {CA+bx8_wx4DnW7P-d4AEAuty0r191PHd12-yH=8JcZcdNwGfU-w-at-mail.gmail.com} } 1, 37 -- Subject: Sudden flashes seen on Fluorescent screen of TEM } 1, 37 -- To: microscopy {microscopy-at-microscopy.com} } 1, 37 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Oct 4 03:20:34 2016 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u948KY8q002505 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 03:20:34 -0500 7, 26 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id u948MPmq006737; 7, 26 -- Tue, 4 Oct 2016 10:22:25 +0200 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id u948MOId020335 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 7, 26 -- Tue, 4 Oct 2016 10:22:25 +0200 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- Subject: Re: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM 7, 26 -- References: {201610040516.u945Gb8X017979-at-ns.microscopy.com} 7, 26 -- To: amit.welcomes.u-at-gmail.com, microscopy-at-microscopy.com 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Message-ID: {84959c59-4adb-d9da-687b-26a7454c774d-at-univ-nantes.fr} 7, 26 -- Date: Tue, 4 Oct 2016 10:22:53 +0200 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 7, 26 -- Thunderbird/45.1.0 7, 26 -- MIME-Version: 1.0 7, 26 -- In-Reply-To: {201610040516.u945Gb8X017979-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 57F366C1.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 57F366C1.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Hi Davide, This pump operates with 200 V or 100 V (see instructions on the plate). It's easy now to find pump that accept large range of voltage. You will have to connect this new pump through the original JEOL connector by recovering the plug from the old pump and reconnecting to the new one according to the JEOL diagrams. That way the TEM will command the pump as before. What is more difficult is to disabled the belt breaking security. The vacuum circuit of the microscope received permanently a signal modulated by the rotation of the pulley of the pump to ensure that the pump is working. Simply disconnecting the cable towards the sensor is not enought. One pin of one of the integrated circuit of the vacuum PB must be removed (lift up), however, with a potential danger for the microscope if the pump stops. I can not answer more precisely without diagrams.
Nicolas STEPHANT
Universit de Nantes Institut Jean Rouxel Service de microscopie lectronique balayage et microanalyse 2 rue de la Houssinire BP 92208 44322 Nantes cdex 3
Tl : 02 40 37 64 26 Ml : nicolas.stephant-at-univ-nantes.fr Web : http://cnrs-imn.fr
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 03/10/2016 19:57, dcristofori-at-unive.it a crit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } we have a small problem with the rotary pump serving our Jeol JEM-3010. } Given the age of the pump, we are evaluating whether try to fix the } problem or buy a new pump. In the latter case, we would prefer to buy a } different brand than the original one provided with the TEM. But we're } not sure about the compatibility of the pumps when dealing with the } control signal used by the TEM electronics to operate the rotary pump. } Does anyone have experience with such an issue? } Any contribution will be greatly appreciated. } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Centro di Microscopia Elettronica "Giovanni Stevanato" e } Dipartimento di Sc. Molecolari e Nanosistemi } Universit Ca' Foscari Venezia } } Campus Scientifico, Edificio ETA } Via Torino 155 I-30172 Mestre (VE) Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } ==============================Original Headers============================== } 7, 46 -- From dcristofori-at-unive.it Mon Oct 3 12:40:59 2016 } 7, 46 -- Received: from mail-wm0-f48.google.com (mail-wm0-f48.google.com [74.125.82.48]) } 7, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u93HevUE009525 } 7, 46 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2016 12:40:59 -0500 } 7, 46 -- Received: by mail-wm0-f48.google.com with SMTP id b201so95507644wmb.0 } 7, 46 -- for {microscopy-at-microscopy.com} ; Mon, 03 Oct 2016 10:42:48 -0700 (PDT) } 7, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 46 -- d=unive-it.20150623.gappssmtp.com; s=20150623; } 7, 46 -- h=from:subject:to:message-id:date:user-agent:mime-version } 7, 46 -- :content-transfer-encoding; } 7, 46 -- bh=eB6mAVy8MvGEovrZ5wyQD8/TkE40k5uCMi2h4TpCk54=; } 7, 46 -- b=daut8ux6LGRuhrcbp4kRw+BA70otBgS2i7oWHht2fWCCkojhDBkDhrDzbPaA+kIxmQ } 7, 46 -- qpNkH1y4DvCy/UsVvL9kgkdCQ5KQ+OAyPCGozRZmbVeJF4I+itDteERKz7+C8lXqMJh3 } 7, 46 -- HzaerJ3LguMzTgnmDi4S+blCRwAsMhWhsTs8ASWOYC8/dOJddXCGcUNuNpW8Z+lXfD6C } 7, 46 -- 2RnSpZ9wmimAPGeJ1eog1ATnkk4SMw6SDC5dezPN/xSFormBfcic3nCRc6uiV4aommRM } 7, 46 -- 0bf8YuUMFC0HSh0kjmBbdzKkjG0Bk1ZZ0JFEL8KH50T+s6VFgnduRp+5zHZk3AmDAsEy } 7, 46 -- DUWQ== } 7, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 46 -- d=1e100.net; s=20130820; } 7, 46 -- h=x-gm-message-state:from:subject:to:message-id:date:user-agent } 7, 46 -- :mime-version:content-transfer-encoding; } 7, 46 -- bh=eB6mAVy8MvGEovrZ5wyQD8/TkE40k5uCMi2h4TpCk54=; } 7, 46 -- b=UxBxPjMqfCEAbWA0ncM++9VbccYnKP+AfHdIDA4Hj6/5b4kKCH3eKDsZ+zwpVmVtvs } 7, 46 -- xzz2yYFEwAzaus3cKNACr8cuBNfavTiQGa8LfrqvX91EqgA0qfngkcv0UXmmMWpEDgkY } 7, 46 -- UKAH08I1taJF33HGSmyiMV2dgLKfA1ta0LSey1w3IDkl5PUgZkD6mhDG/G6NjtuxcX/Z } 7, 46 -- IOW+53rqLjvcQVgpWuEkEGBLKduyC2WdBwmUXwzaml7Vu1ARFuj/L40Y5FZiBADml4n/ } 7, 46 -- Q3kU5fu/eq+GEefg3bjErjth3ngZP4JZ8fPEn6MkOf4iSGXASfB6UafkxVQ6aSAnkCJK } 7, 46 -- ydww== } 7, 46 -- X-Gm-Message-State: AA6/9RnF1Omq2UqXqC6F9b/eBTesWZJaexYYzm5eYt7VyROQN4ENtQKMwva+QXzzDnVHPeKU } 7, 46 -- X-Received: by 10.194.127.5 with SMTP id nc5mr10523000wjb.16.1475501391848; } 7, 46 -- Mon, 03 Oct 2016 06:29:51 -0700 (PDT) } 7, 46 -- Received: from [157.138.23.17] (battagliarin.dcf.unive.it. [157.138.23.17]) } 7, 46 -- by smtp.gmail.com with ESMTPSA id w138sm20037159wmd.1.2016.10.03.06.29.50 } 7, 46 -- for {microscopy-at-microscopy.com} } 7, 46 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 7, 46 -- Mon, 03 Oct 2016 06:29:51 -0700 (PDT) } 7, 46 -- From: Davide Cristofori {dcristofori-at-unive.it} } 7, 46 -- Subject: Rotary pump on Jeol TEM } 7, 46 -- To: microscopy-at-microscopy.com } 7, 46 -- Message-ID: {7b7c4de3-db6c-b1d4-811e-406c6f4dfeea-at-unive.it} } 7, 46 -- Date: Mon, 3 Oct 2016 15:35:42 +0200 } 7, 46 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 } 7, 46 -- Thunderbird/45.3.0 } 7, 46 -- MIME-Version: 1.0 } 7, 46 -- Content-Type: text/plain; charset=iso-8859-15; format=flowed } 7, 46 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Oct 4 03:54:13 2016 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u948sDx8025189 7, 26 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 03:54:13 -0500 7, 26 -- Received: from p-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id u948u5EO007318; 7, 26 -- Tue, 4 Oct 2016 10:56:05 +0200 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by p-smtp.cnrs-imn.fr (8.14.3/8.14.3/Debian-9.4) with ESMTP id u948u4sL020583 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 7, 26 -- Tue, 4 Oct 2016 10:56:05 +0200 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- Subject: Re: [Microscopy] Rotary pump on Jeol TEM 7, 26 -- References: {201610031757.u93Hvf1O021887-at-ns.microscopy.com} 7, 26 -- To: dcristofori-at-unive.it, microscopy-at-microscopy.com 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Message-ID: {7bf53fed-d266-7457-4767-7cec917b56db-at-univ-nantes.fr} 7, 26 -- Date: Tue, 4 Oct 2016 10:56:34 +0200 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 7, 26 -- Thunderbird/45.1.0 7, 26 -- MIME-Version: 1.0 7, 26 -- In-Reply-To: {201610031757.u93Hvf1O021887-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 57F36EA5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 57F36EA5.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/p-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
On Tue, Oct 4, 2016 at 1:52 PM, Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} wrote: } Hi, } I believe you have seen an electric discharge. May be the connection between } the screen and the ground is pretty bad. This connection is done through a } spring under the screen and the circuit for measuring the current received } by the screen. } } Nicolas STEPHANT }
} Hello } } it might be a contamination in the Wehnelt cylinder or equivalent other electron soure } } Jan
Is there any outlet where say I can measure and demonstrate the above? i.e say any output like say ground wire, where i can safely connect a multimeter and check? Also If it's wehnelt contamination etc, can it be remidified without calling engineers? As maintanence contract is not there I would like to provide rough estimate to administration before calling jeol for help
==============================Original Headers============================== 4, 40 -- From amit.welcomes.u-at-gmail.com Tue Oct 4 04:33:04 2016 4, 40 -- Received: from mail-yw0-f170.google.com (mail-yw0-f170.google.com [209.85.161.170]) 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u949X4I4016270 4, 40 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 04:33:04 -0500 4, 40 -- Received: by mail-yw0-f170.google.com with SMTP id g192so124840502ywh.1 4, 40 -- for {microscopy-at-microscopy.com} ; Tue, 04 Oct 2016 02:34:57 -0700 (PDT) 4, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 40 -- d=gmail.com; s=20120113; 4, 40 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to; 4, 40 -- bh=IkFEtV2Yk74maTBcnGndz7+yAo2bGgxqNhhk77zIEfM=; 4, 40 -- b=G6xex0ptmNABPyD0Lf0tXtLbo/Z/i9pibDYt5xNuBOeSmFqmFCVhrieKS3yurgSxla 4, 40 -- 3YNLTHIIYBz2aiqjRfHsyBmbAfXoGjooKyio+YVoHW/DMJOjh3M/JPpSlyF+FE6j4yLY 4, 40 -- cVUcngV7KK0NpOojEE6Jh/0V3zZ2bZi5oCGJvSEdEC8Kb9fYhMLbhN1PzwgBr058VXeN 4, 40 -- tarGwba6KHEGFPAOIfhvLN9FFyiVoPqlAOnnV3pIhcOPiqYGTCAa7+R/oW7QFMIOq9q8 4, 40 -- AOg8kjuq0G/V7OBP+uiwWJneu6BQjRo11UJODE4wATSkTP8ePa95VptXOfDoeGtehCGB 4, 40 -- gIUw== 4, 40 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 40 -- d=1e100.net; s=20130820; 4, 40 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date 4, 40 -- :message-id:subject:to; 4, 40 -- bh=IkFEtV2Yk74maTBcnGndz7+yAo2bGgxqNhhk77zIEfM=; 4, 40 -- b=FEfOfWfWjkx8YkfXWSS4mO8D5SuTq1nohXoVkhy3w96SICpJVeC/mmc0EmcHi2Px0Z 4, 40 -- Y6kKuAvoX0yC0J9YYojhqQp3OBWMb74sGWpwXHu+GbM2vUgb9Qsi1Q1wyjQiAkzjitJc 4, 40 -- 81XJWGEDklFdzD5GrKyA5Ey5h8KnnHNLMIRebAnJskcdA5p280tQVlgiY/8ZTFcXxEMv 4, 40 -- VIxddlczni3SMtpjqai8mtKcNA0O4wa7pyxY0zlmnsNmK88bHuWyMkknjzEomr5ERUP9 4, 40 -- iPPorCeJYoEJhvISHfeEG5Mb7dRVkrfWoARbEhvL0PSy12BV67r2xFE/VDo0rcSjvWdG 4, 40 -- iyjQ== 4, 40 -- X-Gm-Message-State: AA6/9RmUxLd4gJJMItkskeOSdoOXpIhXsfkc/FFcaEUP+jj5Mq9sSz/dQ2Vb6TC5giA0H29YeeqhP0t6Nar9/w== 4, 40 -- X-Received: by 10.37.57.85 with SMTP id g82mr1821565yba.125.1475573697169; 4, 40 -- Tue, 04 Oct 2016 02:34:57 -0700 (PDT) 4, 40 -- MIME-Version: 1.0 4, 40 -- Received: by 10.37.203.139 with HTTP; Tue, 4 Oct 2016 02:34:36 -0700 (PDT) 4, 40 -- In-Reply-To: {84959c59-4adb-d9da-687b-26a7454c774d-at-univ-nantes.fr} 4, 40 -- References: {201610040516.u945Gb8X017979-at-ns.microscopy.com} {84959c59-4adb-d9da-687b-26a7454c774d-at-univ-nantes.fr} 4, 40 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 4, 40 -- Date: Tue, 4 Oct 2016 15:04:36 +0530 4, 40 -- Message-ID: {CA+bx8_wGBP5ghswRLvD=5zDNzfxfdzk+LSFQXUsDhm_EQC0y=w-at-mail.gmail.com} 4, 40 -- Subject: Re: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM 4, 40 -- To: Nicolas.Stephant-at-univ-nantes.fr, microscopy {microscopy-at-microscopy.com} 4, 40 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
If you have arcing in the gun, you should get a Geiger counter and check for radiation. If your image shakes, rattles or rolls, then I might go with the gun.
I agree, however, with the screen posibility. You may see arcs, but do you see the beam move? Or 'flutter' on the screen. Even worse, at high mag, is your focus unstable. If just flashes of light, but not the other phenomena, You might want to vent the camera - viewing area - and check under the screen.
NOTE: Radio Shack has a glass fiber 'pen' that is designed to clean contact surfaces. If the spring is attached to ground, you might save your bosses a visit by cleaning the contact area on the bottom of the screen.
NOTE: I have a rule about electron beams. When they misbehave, I always change the filament first. If there is no change, then, depending on age, I might save the filament.
NOTE: Sometime, the gun housing just needs a good cleaning, but with my FEI/Philips Tecnai, there follows a lot of outgasing even after using the Sun lamp to start the drying out.
Hope some of this helps,
Fred Monson
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu ________________________________________ X-from: amit.welcomes.u-at-gmail.com {amit.welcomes.u-at-gmail.com} Sent: Tuesday, October 4, 2016 5:44 AM To: Monson, Frederick
On Tue, Oct 4, 2016 at 1:52 PM, Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} wrote: } Hi, } I believe you have seen an electric discharge. May be the connection between } the screen and the ground is pretty bad. This connection is done through a } spring under the screen and the circuit for measuring the current received } by the screen. } } Nicolas STEPHANT }
} Hello } } it might be a contamination in the Wehnelt cylinder or equivalent other electron soure } } Jan
Is there any outlet where say I can measure and demonstrate the above? i.e say any output like say ground wire, where i can safely connect a multimeter and check? Also If it's wehnelt contamination etc, can it be remidified without calling engineers? As maintanence contract is not there I would like to provide rough estimate to administration before calling jeol for help
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==============================Original Headers============================== 15, 43 -- From FMonson-at-wcupa.edu Tue Oct 4 14:13:42 2016 15, 43 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 15, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u94JDgEL031891 15, 43 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 14:13:42 -0500 15, 43 -- X-ASG-Debug-ID: 1475608536-08911c175f1d4f1d0001-4CH8be 15, 43 -- Received: from WCUXCHP05.PASSHE.LCL ([144.26.2.141]) by MX01.WCUPA.EDU with ESMTP id TgTrjKU7qgEAkKnz (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO); Tue, 04 Oct 2016 15:15:36 -0400 (EDT) 15, 43 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 15, 43 -- X-Barracuda-Apparent-Source-IP: 144.26.2.141 15, 43 -- X-ASG-Whitelist: Client 15, 43 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP05.PASSHE.LCL 15, 43 -- (144.26.2.141) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Tue, 4 Oct 15, 43 -- 2016 15:15:36 -0400 15, 43 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 15, 43 -- ([144.26.2.144]) with mapi id 15.00.1178.000; Tue, 4 Oct 2016 15:15:36 -0400 15, 43 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 15, 43 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 15, 43 -- CC: "amit.welcomes.u-at-gmail.com" {amit.welcomes.u-at-gmail.com} 15, 43 -- Subject: Re: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM 15, 43 -- Thread-Topic: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of 15, 43 -- TEM 15, 43 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM 15, 43 -- Thread-Index: AQHSHiQhJtxmAVHF20u3LjwEdsbcaqCYWYo7 15, 43 -- Date: Tue, 4 Oct 2016 19:15:35 +0000 15, 43 -- Message-ID: {1475608532616.81272-at-wcupa.edu} 15, 43 -- References: {201610040944.u949iCKX027016-at-ns.microscopy.com} 15, 43 -- In-Reply-To: {201610040944.u949iCKX027016-at-ns.microscopy.com} 15, 43 -- Accept-Language: en-US 15, 43 -- Content-Language: en-US 15, 43 -- X-MS-Has-Attach: 15, 43 -- X-MS-TNEF-Correlator: 15, 43 -- x-ms-exchange-messagesentrepresentingtype: 1 15, 43 -- x-ms-exchange-transport-fromentityheader: Hosted 15, 43 -- x-originating-ip: [144.26.2.59] 15, 43 -- Content-Type: text/plain; charset="iso-8859-1" 15, 43 -- MIME-Version: 1.0 15, 43 -- X-Barracuda-Connect: UNKNOWN[144.26.2.141] 15, 43 -- X-Barracuda-Start-Time: 1475608536 15, 43 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 15, 43 -- X-Barracuda-URL: https://SPAMCONTROL.WCUPA.EDU:443/cgi-mod/mark.cgi 15, 43 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 15, 43 -- X-Barracuda-BRTS-Status: 1 15, 43 -- Content-Transfer-Encoding: 8bit 15, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u94JDgEL031891 ==============================End of - Headers==============================
Since the 2100F is a FEG system, the gun components differ than those described by some of the responses. I would avoid opening the FEG unless directed by service, or if you decide to replace the FEG tip. Replacement of FEG is not trivial (nor cheap), unlike W or LaB6, so this is not where I would start...
The click you are hearing is worrisome. Is it coming from the gun area (top of microscope)? Or perhaps from the HT tank behind the microscope? Try to determine whether the FEG emission current is fluctuating shortly before or after you hear this click. You may need to set up a camera or screen capture software to monitor the emission current (and vacuum levels!).
If the click is coming from the gun area, this is usually indicative of arcing. Bad! Usually caused by lint/dust/particulate contamination in the gun region. This would be unusual for a FEG. Has the FEG been opened in the recent past? If the noise is coming from the HT tank, then you may be experiencing a problem with the tank, which would necessitate a service call.
Since this is the 2100 series, it must be relatively young, correct? Probably not an electronics issue, so I'm not sure why changing C2/C3 (brightness) knob would affect operation.
As other have indicated, it could be related to charging of the InP screens (don't forget little screen). But click noise means, in my opinion, more sinister problem that you need to find. I hope not!
Good luck, Chris
On Tue, Oct 4, 2016 at 5:43 AM, {amit.welcomes.u-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } On Tue, Oct 4, 2016 at 1:52 PM, Nicolas Stephant } {Nicolas.Stephant-at-univ-nantes.fr} wrote: } } Hi, } } I believe you have seen an electric discharge. May be the connection between } } the screen and the ground is pretty bad. This connection is done through a } } spring under the screen and the circuit for measuring the current received } } by the screen. } } } } Nicolas STEPHANT } } } } } Hello } } } } it might be a contamination in the Wehnelt cylinder or equivalent other electron soure } } } } Jan } } } Is there any outlet where say I can measure and demonstrate the above? } i.e say any output like say ground wire, where i can safely connect a } multimeter and check? } Also If it's wehnelt contamination etc, can it be remidified without } calling engineers? As maintanence contract is not there I would like } to provide rough estimate to administration before calling jeol for } help } } ==============================Original Headers============================== } 4, 40 -- From amit.welcomes.u-at-gmail.com Tue Oct 4 04:33:04 2016 } 4, 40 -- Received: from mail-yw0-f170.google.com (mail-yw0-f170.google.com [209.85.161.170]) } 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u949X4I4016270 } 4, 40 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2016 04:33:04 -0500 } 4, 40 -- Received: by mail-yw0-f170.google.com with SMTP id g192so124840502ywh.1 } 4, 40 -- for {microscopy-at-microscopy.com} ; Tue, 04 Oct 2016 02:34:57 -0700 (PDT) } 4, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 40 -- d=gmail.com; s=20120113; } 4, 40 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to; } 4, 40 -- bh=IkFEtV2Yk74maTBcnGndz7+yAo2bGgxqNhhk77zIEfM=; } 4, 40 -- b=G6xex0ptmNABPyD0Lf0tXtLbo/Z/i9pibDYt5xNuBOeSmFqmFCVhrieKS3yurgSxla } 4, 40 -- 3YNLTHIIYBz2aiqjRfHsyBmbAfXoGjooKyio+YVoHW/DMJOjh3M/JPpSlyF+FE6j4yLY } 4, 40 -- cVUcngV7KK0NpOojEE6Jh/0V3zZ2bZi5oCGJvSEdEC8Kb9fYhMLbhN1PzwgBr058VXeN } 4, 40 -- tarGwba6KHEGFPAOIfhvLN9FFyiVoPqlAOnnV3pIhcOPiqYGTCAa7+R/oW7QFMIOq9q8 } 4, 40 -- AOg8kjuq0G/V7OBP+uiwWJneu6BQjRo11UJODE4wATSkTP8ePa95VptXOfDoeGtehCGB } 4, 40 -- gIUw== } 4, 40 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 40 -- d=1e100.net; s=20130820; } 4, 40 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date } 4, 40 -- :message-id:subject:to; } 4, 40 -- bh=IkFEtV2Yk74maTBcnGndz7+yAo2bGgxqNhhk77zIEfM=; } 4, 40 -- b=FEfOfWfWjkx8YkfXWSS4mO8D5SuTq1nohXoVkhy3w96SICpJVeC/mmc0EmcHi2Px0Z } 4, 40 -- Y6kKuAvoX0yC0J9YYojhqQp3OBWMb74sGWpwXHu+GbM2vUgb9Qsi1Q1wyjQiAkzjitJc } 4, 40 -- 81XJWGEDklFdzD5GrKyA5Ey5h8KnnHNLMIRebAnJskcdA5p280tQVlgiY/8ZTFcXxEMv } 4, 40 -- VIxddlczni3SMtpjqai8mtKcNA0O4wa7pyxY0zlmnsNmK88bHuWyMkknjzEomr5ERUP9 } 4, 40 -- iPPorCeJYoEJhvISHfeEG5Mb7dRVkrfWoARbEhvL0PSy12BV67r2xFE/VDo0rcSjvWdG } 4, 40 -- iyjQ== } 4, 40 -- X-Gm-Message-State: AA6/9RmUxLd4gJJMItkskeOSdoOXpIhXsfkc/FFcaEUP+jj5Mq9sSz/dQ2Vb6TC5giA0H29YeeqhP0t6Nar9/w== } 4, 40 -- X-Received: by 10.37.57.85 with SMTP id g82mr1821565yba.125.1475573697169; } 4, 40 -- Tue, 04 Oct 2016 02:34:57 -0700 (PDT) } 4, 40 -- MIME-Version: 1.0 } 4, 40 -- Received: by 10.37.203.139 with HTTP; Tue, 4 Oct 2016 02:34:36 -0700 (PDT) } 4, 40 -- In-Reply-To: {84959c59-4adb-d9da-687b-26a7454c774d-at-univ-nantes.fr} } 4, 40 -- References: {201610040516.u945Gb8X017979-at-ns.microscopy.com} {84959c59-4adb-d9da-687b-26a7454c774d-at-univ-nantes.fr} } 4, 40 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} } 4, 40 -- Date: Tue, 4 Oct 2016 15:04:36 +0530 } 4, 40 -- Message-ID: {CA+bx8_wGBP5ghswRLvD=5zDNzfxfdzk+LSFQXUsDhm_EQC0y=w-at-mail.gmail.com} } 4, 40 -- Subject: Re: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM } 4, 40 -- To: Nicolas.Stephant-at-univ-nantes.fr, microscopy {microscopy-at-microscopy.com} } 4, 40 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers==============================
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It is a field emission gun as many noted hence no question of filaments etc.
} } If you have arcing in the gun, you should get a Geiger counter and check for radiation. If your image } } shakes, rattles or rolls, then I might go with the gun.
This looks simple enough! Though just curious, almost all enclosures in TEM are heavily shielded with lead right? If there are any flashes in gun, how can Geiger counter detect it? I mean will any stray radiation make its way out?
Also I called one of engineer today they said in case of any arcing etc in gun, emission is most likely to go off (I desperately want it to be not gun fault!)
} } I agree, however, with the screen posibility. } } You may see arcs, but do you see the beam move? Or 'flutter' on the screen. Even worse, at high mag, is } } your focus unstable. If just flashes of light, but not the other phenomena, You might want to vent the camera } } ?} } viewing area - and check under the screen.
As such there is no impact on image, but I will carry out more thorough observation today to see.
} } One more idea. Maybe the click you hear is an electronics relay } } opening and closing because of electronics malfunction. This is the } } best case. Should be easy to find with a set of good ears, } } stethoscope, or helpful review of the wiring diagrams showing control } } panel (subsystem) circuits.
Malfunctioning relay does not occur to me. I will try it.
} } The click you are hearing is worrisome. Is it coming from the gun area } } (top of microscope)? Or perhaps from the HT tank behind the } } microscope? Try to determine whether the FEG emission current is } } fluctuating shortly before or after you hear this click. You may need } } to set up a camera or screen capture software to monitor the emission } } current (and vacuum levels!).
JEOL software does provide an on screen reading of current of electrons falling on screen, as such I could not detect any fluctuations in them. If arcing is in gun, then JEOL engineers told that it will shut down the emission.
} } If the click is coming from the gun area, this is usually indicative } } of arcing. Bad! Usually caused by lint/dust/particulate contamination } } n the gun region. This would be unusual for a FEG. Has the FEG been } } opened in the recent past? If the noise is coming from the HT tank, } } then you may be experiencing a problem with the tank, which would } } necessitate a service call.
No. FEG was not opened any time in past few years.
} } Since this is the 2100 series, it must be relatively young, correct? } } Probably not an electronics issue, so I'm not sure why changing C2/C3 } } (brightness) knob would affect operation.
Commissioned in 2011. I am not entirely sure of correlation with C2/C3 as well, but if it is charging of screen in such case increasing brightness will increase chanses of discharge. Would it not?
} } As other have indicated, it could be related to charging of the InP } } screens (don't forget little screen). But click noise means, in my } } opinion, more sinister problem that you need to find. I hope not!
} } Good luck, } } Chris
} } Since it changes with screen brightness it is most likely charging of the } } viewing glass. Has someone had the viewing glass out recently for some } } reason?
No. operating column, including screen chamber has not been opened in past 3-4 years as per my knowledge/
} } The viewing glass has a conductive coating on the vacuum side to dissipate } } any charge that may build up from the beam. A small spring touches the glass } } in the lower left corner providing a path to ground for any charge. } } If the glass is reversed or the spring is missing you will experience the } } phenomena you describe. } } To check the glass - use an ohm meter to check the continuity of the glass } } on the atmosphere side. Just touch both leads anywhere on the glass. If you } } have continuity then the glass is backwards and needs to be reversed. If it } } shows open (no continuity) then look to see if the spring is visible in the } } lower left corner of the glass.
I will check for the spring. Also like chris suggested above for checking discharges with Geiger counter, would discharges in screen chamber will also show up on Geiger counter?
} } Regards, } } Joe
Thank you everyone for help, I will keep posted
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Besides the suggestions you have already received, when was the last time the gun and column were baked? The clicking and fluctuating condensers make me wonder if there is a bit of contamination on the condenser aperture. Baking might take care of that.
Did this problem start after a particular sample was imaged? One that might cause contamination?
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 ________________________________________ X-from: amit.welcomes.u-at-gmail.com {amit.welcomes.u-at-gmail.com} Sent: Wednesday, October 5, 2016 02:19 To: Oshel, Philip Eugene
Sorry for the delayed reply,
It is a field emission gun as many noted hence no question of filaments etc.
} } If you have arcing in the gun, you should get a Geiger counter and check for radiation. If your image } } shakes, rattles or rolls, then I might go with the gun.
This looks simple enough! Though just curious, almost all enclosures in TEM are heavily shielded with lead right? If there are any flashes in gun, how can Geiger counter detect it? I mean will any stray radiation make its way out?
Also I called one of engineer today they said in case of any arcing etc in gun, emission is most likely to go off (I desperately want it to be not gun fault!)
} } I agree, however, with the screen posibility. } } You may see arcs, but do you see the beam move? Or 'flutter' on the screen. Even worse, at high mag, is } } your focus unstable. If just flashes of light, but not the other phenomena, You might want to vent the camera } } ?} } viewing area - and check under the screen.
As such there is no impact on image, but I will carry out more thorough observation today to see.
} } One more idea. Maybe the click you hear is an electronics relay } } opening and closing because of electronics malfunction. This is the } } best case. Should be easy to find with a set of good ears, } } stethoscope, or helpful review of the wiring diagrams showing control } } panel (subsystem) circuits.
Malfunctioning relay does not occur to me. I will try it.
} } The click you are hearing is worrisome. Is it coming from the gun area } } (top of microscope)? Or perhaps from the HT tank behind the } } microscope? Try to determine whether the FEG emission current is } } fluctuating shortly before or after you hear this click. You may need } } to set up a camera or screen capture software to monitor the emission } } current (and vacuum levels!).
JEOL software does provide an on screen reading of current of electrons falling on screen, as such I could not detect any fluctuations in them. If arcing is in gun, then JEOL engineers told that it will shut down the emission.
} } If the click is coming from the gun area, this is usually indicative } } of arcing. Bad! Usually caused by lint/dust/particulate contamination } } n the gun region. This would be unusual for a FEG. Has the FEG been } } opened in the recent past? If the noise is coming from the HT tank, } } then you may be experiencing a problem with the tank, which would } } necessitate a service call.
No. FEG was not opened any time in past few years.
} } Since this is the 2100 series, it must be relatively young, correct? } } Probably not an electronics issue, so I'm not sure why changing C2/C3 } } (brightness) knob would affect operation.
Commissioned in 2011. I am not entirely sure of correlation with C2/C3 as well, but if it is charging of screen in such case increasing brightness will increase chanses of discharge. Would it not?
} } As other have indicated, it could be related to charging of the InP } } screens (don't forget little screen). But click noise means, in my } } opinion, more sinister problem that you need to find. I hope not!
} } Good luck, } } Chris
} } Since it changes with screen brightness it is most likely charging of the } } viewing glass. Has someone had the viewing glass out recently for some } } reason?
No. operating column, including screen chamber has not been opened in past 3-4 years as per my knowledge/
} } The viewing glass has a conductive coating on the vacuum side to dissipate } } any charge that may build up from the beam. A small spring touches the glass } } in the lower left corner providing a path to ground for any charge. } } If the glass is reversed or the spring is missing you will experience the } } phenomena you describe. } } To check the glass - use an ohm meter to check the continuity of the glass } } on the atmosphere side. Just touch both leads anywhere on the glass. If you } } have continuity then the glass is backwards and needs to be reversed. If it } } shows open (no continuity) then look to see if the spring is visible in the } } lower left corner of the glass.
I will check for the spring. Also like chris suggested above for checking discharges with Geiger counter, would discharges in screen chamber will also show up on Geiger counter?
} } Regards, } } Joe
Thank you everyone for help, I will keep posted
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I am having a discussion with a colleague about approaches to thin-sectioning teeth (i.e., enamel). Historically, this has been done with decalcification, embedding in MMA resins, then sectioning - meaning months from start to finish. Recently, people have used a cold-polymerizing GMA resin with the commercial name Technovit 8100. Teeth are embedded, cut into preparatory sections (1 mm) with a saw, decalcified, then re-embedded and thin-sectioned on an ultramicrotome using tungsten (and even glass) blades. That process start-to-finish takes a month (simply because of the EDTA decalcification). My colleague, who is a very a talented bone histologist, just says flat out that Technovit is a waste of time because it is GMA and just too soft. Im wondering whether anyone here has first-hand experience with Technovit and would care to comment on its advantages/disadvantages compared to MMA. Specifically, Id love to hear something about relative hardness. In the end, we want to do in-situ hybridization and fluorescence microscopy.
Thanks in advance for your perspectives!
Robert J. Palmer Jr., PhD National Institute of Dental and Craniofacial Research National Institutes of Health Building 30, Room 331 Bethesda MD 20892 301-594-0025
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Email: brian.collins-at-wsu.edu Name: Brian Collins
Message: A Postdoctoral Research Fellow position is available with professor Brian A. Collins, in the Department of Physics at Washington State University (WSU) in partnership with Lawrence Berkeley National Laboratory (LBNL).
Nano-to-mesoscale interactions and structures within organic systems are of critical importance for our understanding of their macroscopic properties and functions, many of which only occur in specific operational environments. Resonant soft X-ray scattering (RSoXS) is uniquely sensitive to bond-type, bond-orientation, and is capable of probing these materials with high sensitivity using their intrinsic chemical structure rather than laborious or disruptive labeling. Currently, however, RSoXS can only be performed on samples suspended in vacuum. The development of environmental control (gases, liquids, temperatures, electric fields, and charges) will allow leaps in our knowledge of interactions and structures of organic materials that are uniquely relevant to the very applications in which they form, exist and function. DUTIES: The successful candidate is expected to develop a new instrument at beamline 11.0.1.2 that incorporates environmental control and stimuli during RSoXS measurements on soft matter systems enabling in-situ and in-operando measurements on materials applicable to organic devices or biological systems. This will involve a new chamber, detector, and a commercial flow apparatus commonly used for transmission electron microscopes (TEMs). The work is expected to involve incorporation of systems, design and lithographic fabrication of environmental cells, and demonstration of the final instrument by applying its capabilities in a scientific project that combines TEM and RSoXS. With RSoXS beamlines being developed at synchrotrons around the world, this work will demonstrate leadership and expertise in a widely expanding field with excellent career opportunities.
REQUIREMENTS: Candidates must have significant experience in either synchrotron X-ray scattering/spectroscopy techniques or in-situ flow-cell experiments in a TEM. Computer program experience is highly encouraged. Candidates must also have a Ph.D. in Physics, Chemistry, Chemical/Materials Engineering, or a related field.
APPOINTMENT: This is a joint appointment between WSU and the LBNL Postdoctoral Fellowship Program with benefits associated with WSU. The salary is $61,200/yr with appropriate experience. The term is for one year, with the possibility of renewal based on satisfactory performance. A few months prep work locally at WSU is preferred with the remainder of the appointment to occur at LBNL. Preferred start date is as soon as available.
HOW TO APPLY: Interested applicants should submit a cover letter, CV (including list of publications), and arrange to have three letters of recommendation sent to Brian A. Collins at brian.collins-at-wsu.edu. Review of applications will begin immediately. The position will remain open until filled. WSU is an EO/AA Educator and Employer.
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Wasahington University
Title-Subject: [Filtered] Research Associate Position in DC
Message: https://www.gwu.jobs/postings/38256
Greetings Microscopists,
I have a position open in my lab for a research associate at the George Washington University in the new Science and Engineering Hall in the Nanofabrication and Imaging Center. If you can prepare samples for EM and want to work in an exciting new lab, then check out the link to the description.
Chris Brantner Senior Research Scientist in Electron Microscopy George Washington University Washington DC
chrisbrantner-at-gwu.edu
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NOTE: This came through Project MICRO. Please respond directly to the OP at the email below. Not to the list.
X-from: Weatherbee, Scott [mailto:scott.weatherbee-at-yale.edu] Sent: Thursday, October 6, 2016 3:54 PM To: AssociationManagement-at-microscopy.org {mailto:AssociationManagement-at-microscopy.org}
X-from: wambrose-at-unc.edu
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: UNC
Title-Subject: [Filtered] remote imaging on Hitachi S4700
Message: We have previously instituted remote imaging on our FEI Quanta 200 for broadcasting live demos to our local school area. We also wanted to do the same with our Hitachi S4700. Although we can load the server/client software (TightVNC) and get a connection, the image does not port to the client pc. We get a "purple screen". Has anyone been able to get the live (or captured) image from the Hitachi onto a network client? Thanks Wallace
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Wallace, I believe you're seeing the effects of hardware-acceleration with the video. What I am guessing is happening is the microscope image is transferred via DMA (direct memory access) to the video card directly, bypassing the operating system. VNC however grabs the video frame buffer from the operating system, which doesn't have any information (because after that image is transported to the video card, the DMA transaction fills in the purple section before being sent out to the monitor).
What I did for an older 1990s FEI FIB 200, was to use a VGA video cable splitter: http://www.siig.com/av-products/splitters-distribution-amplifiers/vga/1x2-compact-vga-splitter.html and then a VGA2USB frame grabber: https://www.epiphan.com/products/vga2usb/
Then get another computer, an old desktop or laptop or even a new mini-PC like an Intel NUC or compute-stick or RasperryPi ( {=$40), which can stream/save the video however you like.
You'll just need to get a splitter and grabber for your specific video cable (I'm guessing VGA or DVI): https://www.epiphan.com/products/#usb-video-grabbers
-Nathan
On Wed, Oct 12, 2016 at 6:57 PM, {microscopy.listserver-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: wambrose-at-unc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } wambrose-at-unc.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: wambrose-at-unc.edu Name: Wallace Ambrose } } Organization: UNC } } Title-Subject: [Filtered] remote imaging on Hitachi S4700 } } Message: We have previously instituted remote imaging on our FEI Quanta 200 for broadcasting live } demos to our local school area. We also wanted to do the same with our Hitachi S4700. Although we } can load the server/client software (TightVNC) and get a connection, the image does not port to the } client pc. We get a "purple screen". Has anyone been able to get the live (or captured) image from } the Hitachi onto a network client? } Thanks } Wallace } } Login Host: 152.19.204.249 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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We have a problem with an older Noran Si(Li) detector running on a Voyager platform (it is about 19 years old!) that I have not come across before.
Suddenly this week instead of getting a single peak, all of the peaks in the spectrum are split into doublets. For instance for Al K at 1.486keV we get two peaks separated by ~100eV and centered at 1.486keV.
Has anybody seen this and is there a cure?
Alan
-- Alan W Nicholls, PhD Associate Director, RRC Director Research Resource Facility - Electron Microscopy Research Resources Center, University of Illinois at Chicago Rm 110, 845 West Taylor St Chicago, IL 60607 312 996 1227 nicholls-at-uic.edu
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We've recently bought a K-Kit starter set from EMS. The K-Kit allows the viewing of wet samples in the TEM. I would like to hear from anyone else out there who has used it to get your thoughts and experiences from using it. Any and all information would be welcomed, as usual please respond privately.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 6, 36 -- From tbargar-at-unmc.edu Thu Oct 13 10:12:59 2016 6, 36 -- Received: from zixvpm02.unmc.edu (mysecuremail.unmc.edu [192.198.54.127]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9DFCxwK029858 6, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 10:12:59 -0500 6, 36 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 6, 36 -- by Outbound.unmc.edu (Proprietary) with SMTP id 542562AE540 6, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 09:43:06 -0500 (CDT) 6, 36 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 6, 36 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 6, 36 -- (No client certificate requested) 6, 36 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id 8DD942AE53E 6, 36 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 09:43:04 -0500 (CDT) 6, 36 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 6, 36 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 6, 36 -- 14.03.0301.000; Thu, 13 Oct 2016 10:15:24 -0500 6, 36 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Subject: like to hear from users of the K-Kit 6, 36 -- Thread-Topic: like to hear from users of the K-Kit 6, 36 -- Thread-Index: AdIlZAOseuO2Y//NSziiIoH1KAlINw== 6, 36 -- Date: Thu, 13 Oct 2016 15:15:24 +0000 6, 36 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0B5B04D73-at-UNMCEX2.unmcresforest.org} 6, 36 -- Accept-Language: en-US 6, 36 -- Content-Language: en-US 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- x-originating-ip: [10.8.64.15] 6, 36 -- Content-Type: text/plain; charset="us-ascii" 6, 36 -- MIME-Version: 1.0 6, 36 -- X-VPM-MSG-ID: b4066ab0-1d85-488c-8b42-dae8d509e594 6, 36 -- X-VPM-HOST: zixvpm02.unmc.edu 6, 36 -- X-VPM-GROUP-ID: 91233308-7701-474e-a420-44dba4d7f36c 6, 36 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 6, 36 -- X-VPM-IS-HYBRID: 0 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9DFCxwK029858 ==============================End of - Headers==============================
Sixteen years out from any contact with Amenex's ETEC Autoprobe, I recall seeing line doubling when the detector was getting overloaded, especially in my hands. The context is that I was trying to write code in TrueBasic to automate the process of setting the wavelength-dispersive spectrometers at the correct angle to detect the X-rays coming from specific elements, whose wavelengths are ever so slightly dependent on their chemical surroundings. The software was to scan across the selected wavelength and then select the angle at which the signal was at its maximum. Whenever the detector gain was excessive, the peak would get folded over onto itself, producing the false doublet.
Just back off the gain setting.
Hope this helps.
George Langford, Sc.D. Amenex Associates, Inc. http://www.amenex.com/ amenex-at-amenex.com You wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Colleagues, } } We have a problem with an older Noran Si(Li) detector running on a } Voyager } platform (it is about 19 years old!) that I have not come across } before. } } Suddenly this week instead of getting a single peak, all of the peaks } in } the spectrum are split into doublets. For instance for Al K at 1.486keV } we } get two peaks separated by ~100eV and centered at 1.486keV. } } Has anybody seen this and is there a cure? } } Alan } } } -- } Alan W Nicholls, PhD } Associate Director, RRC } Director Research Resource Facility - Electron Microscopy } Research Resources Center, University of Illinois at Chicago } Rm 110, 845 West Taylor St } Chicago, IL 60607 } 312 996 1227 } nicholls-at-uic.edu } } } ==============================Original } Headers============================== } 8, 25 -- From nicholls-at-uic.edu Thu Oct 13 08:26:04 2016 } 8, 25 -- Received: from smtp-auth-0.cc.uic.edu (smtp-auth-0.cc.uic.edu } [128.248.156.44]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u9DDQ4A3003138 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 08:26:04 } -0500 } 8, 25 -- Received: from smtpout-1.cc.uic.edu } (smtpout-1.data.cc.uic.edu [192.168.100.232]) } 8, 25 -- by smtp-auth-0.cc.uic.edu (Postfix) with SMTP id 436F8875C1 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 08:28:31 } -0500 (CDT) } 8, 25 -- Received: (qmail 1568 invoked from network); 13 Oct 2016 } 08:28:31 -0500 } 8, 25 -- Received: from mail-5.data.cc.uic.edu (HELO webmail.uic.edu) } (192.168.101.22) } 8, 25 -- by smtpout-1.data.cc.uic.edu with SMTP; 13 Oct 2016 08:28:31 } -0500 } 8, 25 -- Received: from 131-193-156-254.pclabs.uic.edu } ([131.193.156.254]) } 8, 25 -- (SquirrelMail authenticated user nicholls) } 8, 25 -- by webmail.uic.edu with HTTP; } 8, 25 -- Thu, 13 Oct 2016 08:28:31 -0500 } 8, 25 -- Message-ID: } {c51757dbc70e759dc7ee860ee43d3aa2.squirrel-at-webmail.uic.edu} } 8, 25 -- Date: Thu, 13 Oct 2016 08:28:31 -0500 } 8, 25 -- Subject: XEDS: Problem with peak splitting } 8, 25 -- From: "Alan W. Nicholls" {nicholls-at-uic.edu} } 8, 25 -- To: microscopy-at-microscopy.com } 8, 25 -- User-Agent: SquirrelMail/1.4.20 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain;charset=iso-8859-1 } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-Priority: 3 (Normal) } 8, 25 -- Importance: Normal } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 33 -- From amenex-at-amenex.com Thu Oct 13 11:52:13 2016 5, 33 -- Received: from ld120.inmotionhosting.com (ld120.inmotionhosting.com [74.124.198.158]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9DGqDqB023398 5, 33 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 11:52:13 -0500 5, 33 -- Received: from [::1] (port=50656 helo=ld120.inmotionhosting.com) 5, 33 -- by ld120.inmotionhosting.com with esmtpa (Exim 4.87) 5, 33 -- (envelope-from {amenex-at-amenex.com} ) 5, 33 -- id 1bujHA-0007dh-8B; Thu, 13 Oct 2016 09:54:40 -0700 5, 33 -- MIME-Version: 1.0 5, 33 -- Content-Type: text/plain; charset=US-ASCII; 5, 33 -- format=flowed 5, 33 -- Content-Transfer-Encoding: 7bit 5, 33 -- Date: Thu, 13 Oct 2016 09:54:36 -0700 5, 33 -- From: amenex-at-amenex.com 5, 33 -- To: "Alan W. Nicholls" {nicholls-at-uic.edu} 5, 33 -- Cc: microscopy-at-microscopy.com 5, 33 -- Subject: Re: [Microscopy] XEDS: Problem with peak splitting 5, 33 -- In-Reply-To: {201610131343.u9DDhNsV021596-at-ns.microscopy.com} 5, 33 -- References: {201610131343.u9DDhNsV021596-at-ns.microscopy.com} 5, 33 -- Message-ID: {7d1e6cf03128f362674b04faf01a8154-at-amenex.com} 5, 33 -- X-Sender: amenex-at-amenex.com 5, 33 -- User-Agent: Roundcube Webmail/1.1.4 5, 33 -- X-OutGoing-Spam-Status: No, score=0.5 5, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 5, 33 -- X-AntiAbuse: Primary Hostname - ld120.inmotionhosting.com 5, 33 -- X-AntiAbuse: Original Domain - microscopy.com 5, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 5, 33 -- X-AntiAbuse: Sender Address Domain - amenex.com 5, 33 -- X-Get-Message-Sender-Via: ld120.inmotionhosting.com: authenticated_id: amenex-at-amenex.com 5, 33 -- X-Authenticated-Sender: ld120.inmotionhosting.com: amenex-at-amenex.com 5, 33 -- X-Source: 5, 33 -- X-Source-Args: 5, 33 -- X-Source-Dir: ==============================End of - Headers==============================
I’m preparing a submission for Microscopy and Microanalysis and I’m looking for a LaTeX template. Does anyone have one they could share or suggestions for another package to use?
Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
Are you talking about overflow in the MCA channels? I know we had an issue with that with a Tracor Northern 2000 system from back around 1980. We had a choice of configuration between 24 bits per channel at 1024 channels or 16 bits per channel at 1536 channels. We opted for the second case and could get rollover in extreme cases. However, I think it looked different that what Nicholl's group has run into.
Warren
________________________________________ X-from: amenex-at-amenex.com {amenex-at-amenex.com} Sent: Thursday, October 13, 2016 11:53 AM To: Straszheim, Warren E [BIOTC]
Dear Dr.Nicholls -
Sixteen years out from any contact with Amenex's ETEC Autoprobe, I recall seeing line doubling when the detector was getting overloaded, especially in my hands. The context is that I was trying to write code in TrueBasic to automate the process of setting the wavelength-dispersive spectrometers at the correct angle to detect the X-rays coming from specific elements, whose wavelengths are ever so slightly dependent on their chemical surroundings. The software was to scan across the selected wavelength and then select the angle at which the signal was at its maximum. Whenever the detector gain was excessive, the peak would get folded over onto itself, producing the false doublet.
Just back off the gain setting.
Hope this helps.
George Langford, Sc.D. Amenex Associates, Inc. http://www.amenex.com/ amenex-at-amenex.com You wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Colleagues, } } We have a problem with an older Noran Si(Li) detector running on a } Voyager } platform (it is about 19 years old!) that I have not come across } before. } } Suddenly this week instead of getting a single peak, all of the peaks } in } the spectrum are split into doublets. For instance for Al K at 1.486keV } we } get two peaks separated by ~100eV and centered at 1.486keV. } } Has anybody seen this and is there a cure? } } Alan } } } -- } Alan W Nicholls, PhD } Associate Director, RRC } Director Research Resource Facility - Electron Microscopy } Research Resources Center, University of Illinois at Chicago } Rm 110, 845 West Taylor St } Chicago, IL 60607 } 312 996 1227 } nicholls-at-uic.edu } } } ==============================Original } Headers============================== } 8, 25 -- From nicholls-at-uic.edu Thu Oct 13 08:26:04 2016 } 8, 25 -- Received: from smtp-auth-0.cc.uic.edu (smtp-auth-0.cc.uic.edu } [128.248.156.44]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u9DDQ4A3003138 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 08:26:04 } -0500 } 8, 25 -- Received: from smtpout-1.cc.uic.edu } (smtpout-1.data.cc.uic.edu [192.168.100.232]) } 8, 25 -- by smtp-auth-0.cc.uic.edu (Postfix) with SMTP id 436F8875C1 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 08:28:31 } -0500 (CDT) } 8, 25 -- Received: (qmail 1568 invoked from network); 13 Oct 2016 } 08:28:31 -0500 } 8, 25 -- Received: from mail-5.data.cc.uic.edu (HELO webmail.uic.edu) } (192.168.101.22) } 8, 25 -- by smtpout-1.data.cc.uic.edu with SMTP; 13 Oct 2016 08:28:31 } -0500 } 8, 25 -- Received: from 131-193-156-254.pclabs.uic.edu } ([131.193.156.254]) } 8, 25 -- (SquirrelMail authenticated user nicholls) } 8, 25 -- by webmail.uic.edu with HTTP; } 8, 25 -- Thu, 13 Oct 2016 08:28:31 -0500 } 8, 25 -- Message-ID: } {c51757dbc70e759dc7ee860ee43d3aa2.squirrel-at-webmail.uic.edu} } 8, 25 -- Date: Thu, 13 Oct 2016 08:28:31 -0500 } 8, 25 -- Subject: XEDS: Problem with peak splitting } 8, 25 -- From: "Alan W. Nicholls" {nicholls-at-uic.edu} } 8, 25 -- To: microscopy-at-microscopy.com } 8, 25 -- User-Agent: SquirrelMail/1.4.20 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Type: text/plain;charset=iso-8859-1 } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-Priority: 3 (Normal) } 8, 25 -- Importance: Normal } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 33 -- From amenex-at-amenex.com Thu Oct 13 11:52:13 2016 5, 33 -- Received: from ld120.inmotionhosting.com (ld120.inmotionhosting.com [74.124.198.158]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9DGqDqB023398 5, 33 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2016 11:52:13 -0500 5, 33 -- Received: from [::1] (port=50656 helo=ld120.inmotionhosting.com) 5, 33 -- by ld120.inmotionhosting.com with esmtpa (Exim 4.87) 5, 33 -- (envelope-from {amenex-at-amenex.com} ) 5, 33 -- id 1bujHA-0007dh-8B; Thu, 13 Oct 2016 09:54:40 -0700 5, 33 -- MIME-Version: 1.0 5, 33 -- Content-Type: text/plain; charset=US-ASCII; 5, 33 -- format=flowed 5, 33 -- Content-Transfer-Encoding: 7bit 5, 33 -- Date: Thu, 13 Oct 2016 09:54:36 -0700 5, 33 -- From: amenex-at-amenex.com 5, 33 -- To: "Alan W. Nicholls" {nicholls-at-uic.edu} 5, 33 -- Cc: microscopy-at-microscopy.com 5, 33 -- Subject: Re: [Microscopy] XEDS: Problem with peak splitting 5, 33 -- In-Reply-To: {201610131343.u9DDhNsV021596-at-ns.microscopy.com} 5, 33 -- References: {201610131343.u9DDhNsV021596-at-ns.microscopy.com} 5, 33 -- Message-ID: {7d1e6cf03128f362674b04faf01a8154-at-amenex.com} 5, 33 -- X-Sender: amenex-at-amenex.com 5, 33 -- User-Agent: Roundcube Webmail/1.1.4 5, 33 -- X-OutGoing-Spam-Status: No, score=0.5 5, 33 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 5, 33 -- X-AntiAbuse: Primary Hostname - ld120.inmotionhosting.com 5, 33 -- X-AntiAbuse: Original Domain - microscopy.com 5, 33 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 5, 33 -- X-AntiAbuse: Sender Address Domain - amenex.com 5, 33 -- X-Get-Message-Sender-Via: ld120.inmotionhosting.com: authenticated_id: amenex-at-amenex.com 5, 33 -- X-Authenticated-Sender: ld120.inmotionhosting.com: amenex-at-amenex.com 5, 33 -- X-Source: 5, 33 -- X-Source-Args: 5, 33 -- X-Source-Dir: ==============================End of - Headers==============================
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ciasem2017-at-imre.uh.cu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ciasem2017-at-imre.uh.cu Name: Carlos Alberto Lariot
Organization: Cuban Society of Microscopy
Title-Subject: [Filtered] CIASEM 2017
Message: As president of the Cuban Society for Microscopy I have the pleasure to invite you to participate in the XIV Congress of the Interamerican Committee of Societies for Microscopy CIASEM 2017, to be held in Varadero beach, Cuba, from September 25 to 29, 2017. Answering to our proposal, Professor Alwyn Eades has wrote the next note: CIASEM 2017
CIASEM is the Inter-American Committee of Societies for Microscopy. It organizes a Congress every two years. The next CIASEM Congress will be in Cuba from September 25 to 29 next year. The organizers are hoping that, now that it is easier for people to travel to Cuba from the USA, microscopists working in the United States will think to attend.
The Inter-American Congresses on Microscopy bring together scientists from many countries in the Americas, from Canada to Argentina. They are always good fun. It is my experience that the discussions at the poster sessions, for example, are far more lively and productive than at other meetings I have attended. As an added incentive, let me mention that the Congress will be held at Varadero, a beautiful Caribbean beach resort.
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It's been a while since I've posted here. I'd previously been maintaining a Leica LEO S430 SEM at a makerspace in Chicago. Recently, we moved the SEM to Analytics Lounge, a new 501(c)(3) non-profit makerspace in Chicago focused on science education and creating a laboratory for citizen science. We also have an ICP-MS that we're trying to bring online, a GFAAS that we're trying to repair, optical microscopes, and gamma and alpha spectroscopy equipment.
I'd heard that the Oxford Link ISIS was capable of controlling the SEM and capturing secondary electron video images, and that the quality of these images was better than what the S430 itself can do, e.g. 1024x768x8 bit.
I don't think we have the necessary hardware for that. Our card cage has a PULSE PROCESSOR, an X-RAY ADC, and an X-ACQ board. But it seems our X-ACQ doesn't have a daughterboard that has a "Microscope Digital Mapping Signal" connector. I'm guessing we'd need the board, and a cable that would connect to the Video Out, Ext Scan Enable, Ext Line, and Ext Frame BNC outputs on the S430. And we'd need a serial connection between the S430 and the Link ISIS. Beyond that, what else would be needed? Is this something that requires a separate license within the Link ISIS software? Would there need to be some configuration files specific to the S430? Assuming we could do this, how much better is the image resolution and quality?
I've also seen a different configuration of cards when I look for info on Oxford Link ISIS: a DXP50, XAC-II, and SCANRAM-II. What's the difference between these and what we have?
Is it possible to obtain Oxford Link ISIS software license files, considering how old this equipment is? In particular, the installation we have won't let me run SEMQuant except in demo mode. I don't know if that was all the original owner purchased, or if we didn't get all the original licenses installed.
I'm likewise interested in whether it is possible to obtain licenses for certain features on the LEO S430. E.g. I think beam rotation is an optional feature that we don't have.
I'm pretty sure we do have the license for RS-232 control of the S430. But I haven't been able to find any documentation on the API. Is this available? We've been considering the possibility of interfacing a MIDI control pad to the SEM via a microcontroller to make it more intuitive to use than its native Windows 3.1 interface.
I know this is seriously old (ca. 1993) hardware and I'm not sure how many people are still using it, but any available information would be much appreciated.
Thanks,
Ryan
==============================Original Headers============================== 11, 17 -- From rdpierce-at-pobox.com Sat Oct 15 13:15:52 2016 11, 17 -- Received: from elna.mackenziegems.com (elna.mackenziegems.com [66.253.36.183]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9FIFqcu015027 11, 17 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2016 13:15:52 -0500 11, 17 -- Received: from [127.0.0.1] (localhost [127.0.0.1]) 11, 17 -- by elna.mackenziegems.com (Postfix) with ESMTPSA id A11836E3068 11, 17 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2016 13:18:26 -0500 (CDT) 11, 17 -- To: microscopy-at-microscopy.com 11, 17 -- From: Ryan Pierce {rdpierce-at-pobox.com} 11, 17 -- Subject: Info on Leica LEO S430 and Oxford Link ISIS EDX 11, 17 -- Message-ID: {aa6968a2-0fbb-3a8f-8be8-665eff0908b9-at-pobox.com} 11, 17 -- Date: Sat, 15 Oct 2016 13:18:26 -0500 11, 17 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 11, 17 -- Thunderbird/45.2.0 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; charset=utf-8; format=flowed 11, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The registration deadline to join the Pacific NW Microscopy Symposium has been extended to 5PM October 27th. This is a two-day symposium to be held November 3rd and 4th in Portland, Oregon. Registration for the Symposium is included with a one-year membership in the Pacific NW Microscopy Society ($25 for professionals; $15 for students) and includes the symposia, workshops, reception and dinner, a farewell wine and cheese social, and all meals during the meeting. The program is absolutely superb. Please go to the PacificNWMicroscopy Website to view the Program and register for the event.
Here is the link: www.pacificnwmicroscopy.org
We will look forward to seeing you in Portland!
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3101 SW Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Mobile: 503-819-3600
Dear Listers, Sorry for not replying till now. Quite busy days! I'd like to thank everybody contributed to the rotary pump substitution discussion. We finally decided how to manage the issue, thanks to your comments: as pointed out by some of you, the control wires doesn't just give consensus to the alimentation of the pump, but are connected to a sensor for the rotation of the pulley of the pump. I was able to find the sensor in the pump. We don't feel comfortable about both modifying the electronics of the instrument, and introducing a potential danger for the TEM, so decided to (try to) repair the pump. Thanks again.
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Organization: CIGS-University of Modena Title-Subject: [Filtered] EDS detector shutter
Message: Hello, I need technical help about an old Oxford EDS detector. The detector is installed on a Jeol 2010 TEM; the microscope has been under maintenance for a long period but now it's fine. The detector problem is: when I switch ON the PC/interface unit, the software starts and the shutter seems to be OPEN (?) but when I try to CLOSE it the error message "shutter failure" appears and a buzzer-alarm sounds. It's necessary to switch off the PC/interface unit to interrupt the alarm. Anyone ever faced this problem? The shutter system seems a pneumatic system driven through a gray box mounted on the detector metallic bellow. Anybody knows if is a risky job (for the detector) to open this box for checking?
Thanks so much in advance,
Massimo
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Email: ciasem2017-at-imre.uh.cu Name: Carlos Alberto Lariot
Organization: Organization Committee of the CIASEM 2017
Title-Subject: [Filtered] Announcement Message: I have the pleasure to invite you to participate in the XIV Congress of the Interamerican Committee of Societies for Microscopy CIASEM 2017, to be held in Varadero beach, Cuba, from September 25 to 29, 2017. Answering to CIASEM 2017 Organization Committee, Professor Alwyn Eades has wrote the next note:
CIASEM is the Inter-American Committee of Societies for Microscopy. It organizes a Congress every two years. The next CIASEM Congress will be in Cuba from September 25 to 29 next year. The organizers are hoping that, now that it is easier for people to travel to Cuba from the USA, microscopists working in the United States will think to attend.
The Inter-American Congresses on Microscopy bring together scientists from many countries in the Americas, from Canada to Argentina. They are always good fun. It is my experience that the discussions at the poster sessions, for example, are far more lively and productive than at other meetings I have attended. As an added incentive, let me mention that the Congress will be held at Varadero, a beautiful Caribbean beach resort.
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==============================Original Headers============================== 16, 52 -- From microscopy.listserver-at-gmail.com Mon Oct 17 07:28:12 2016 16, 52 -- Received: from mail-it0-f41.google.com (mail-it0-f41.google.com [209.85.214.41]) 16, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9HCSCtS006697 16, 52 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2016 07:28:12 -0500 16, 52 -- Received: by mail-it0-f41.google.com with SMTP id k64so12688120itb.0 16, 52 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2016 05:30:53 -0700 (PDT) 16, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 52 -- d=gmail.com; s=20120113; 16, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 16, 52 -- bh=cj9djXr7l6DEIlWY11Nbtf7fw8qGyJLvZZo0VFdhq6E=; 16, 52 -- b=mGo2huNCsyvtSvghUHJATwsyZr1LWzpG1STPolPRpR9q8rlzGsWc5PTiVPsoDR9wV8 16, 52 -- Ei4W32df+Q/kaMfD4ny+10zRDFOLZeSIaPW4g5DDxfGk9IPSqxnewHzyp7bsUA+udQpP 16, 52 -- sz3YhftoELs99HUdOcHCLWx6thOx0GWqZGHY4aGFyV7M2w3eV5XU618ds+03aQWJafoE 16, 52 -- dEReaDKzwgxQkVN9dylmelKqGLrsUiAxgtAmcBFB+6JNUBqGCQPbFCQXmZ5/59vNaf53 16, 52 -- fEc/zL3590C08GcZJyY9uZa9m774IeWf28cB8wrdPRQu1tjuWN+4iZStwo/wbhMwFVGD 16, 52 -- c2JQ== 16, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 52 -- d=1e100.net; s=20130820; 16, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 16, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 16, 52 -- bh=cj9djXr7l6DEIlWY11Nbtf7fw8qGyJLvZZo0VFdhq6E=; 16, 52 -- b=RHLwb7lzv4fvQC8mIPa4jWjelTHS22oc3umx8q5YSN6RC8opMX44ACxjULtMNayj9n 16, 52 -- g9vZnn9xovD1HKBPwajE7de2AkhF9Y8TvtN9fNJU7pRTBkKkHxfnasgWqJvv6WrKQYwL 16, 52 -- jKNXjja/dA/kWg0Jz85VmfDHbtU+LWa9Hra1EN0AqDDjOM4L9GApu/MkybjDbjpayACp 16, 52 -- H5fsM9rnLGdyaHg64yrwslc3ZARGsOcc8fSS1AlnTJJBRkw9rV80XCP76dGH4H6wYMAV 16, 52 -- EVDA6gQzEH2po9a3DXQqR3O7PtyfP7slS+2jABmxyQU579uVFlyrKOqHTrObLegerCep 16, 52 -- Bllw== 16, 52 -- X-Gm-Message-State: AA6/9Rnt+j445TKtjPjLEcgIE+LmSfBaH/XorpYSN4hCds8jpIWUCXyvILhJoA0nDrGJOA== 16, 52 -- X-Received: by 10.36.91.213 with SMTP id g204mr7654083itb.42.1476707453084; 16, 52 -- Mon, 17 Oct 2016 05:30:53 -0700 (PDT) 16, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([24.15.143.135]) 16, 52 -- by smtp.googlemail.com with ESMTPSA id m31sm13354224ioi.40.2016.10.17.05.30.52 16, 52 -- for {microscopy-at-microscopy.com} 16, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 52 -- Mon, 17 Oct 2016 05:30:52 -0700 (PDT) 16, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 52 -- {microscopylistserver-noreply-at-microscopy.com} 16, 52 -- Subject: viaWWW:CIASEM 2017, to be held in Varadero beach, Cuba 16, 52 -- References: {201610141441.u9EEf32c007639-at-ns.microscopy.com} 16, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 52 -- X-Forwarded-Message-Id: {201610141441.u9EEf32c007639-at-ns.microscopy.com} 16, 52 -- Message-ID: {b7e23567-925e-967c-839c-74b4f1abba90-at-microscopy.com} 16, 52 -- Date: Mon, 17 Oct 2016 07:30:52 -0500 16, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 16, 52 -- Gecko/20100101 Thunderbird/45.4.0 16, 52 -- MIME-Version: 1.0 16, 52 -- In-Reply-To: {201610141441.u9EEf32c007639-at-ns.microscopy.com} 16, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: andy.stewart-at-ul.ie Name: Andy Stewart
Organization: University of Limerick
Title-Subject: [Filtered] Post-doc position available Message: Post Doctoral Researcher Electron Microscopist LOCATION: University of Limerick REPORTS TO: Director of Bernal Institute/Nominee of the Director SALARY: 37,750 - 46,255 p.a. (maximum starting salary 42,394 p.a.)
QUALIFICATIONS: A doctoral degree (level 10 NFQ) in materials science/electron microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent experience in a research environment with at least 2 years experience in the area of electron microscopy applied to materials characterisation.
OVERALL PURPOSE OF THE JOB: To manage and develop the electron microscopy suite at the University of Limerick, to support research activity in the Institute in the area of materials characterisation and to carry out original and significant research in this area. Essential Criteria Applicants must have: A doctoral degree (level 10 NFQ) in materials science/electron microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent experience in a research environment with at least 2 years experience in the area of electron microscopy applied to materials characterisation. Hands-on experience in electron microscopy and be able to train new users in this technique. Demonstrable knowledge of materials science, particularly related to the areas of materials for energy and environment, nanomaterials, pharmaceutical materials, biomedical engineering and biomaterials, and composite materials. Evidence of ability to work individually and as part of a team. Good oral and written communication skills. Potential to write research reports and develop research proposals in the field of materials, in collaboration with other researchers. A strong track record of recent publications in peer reviewed journals, with at least three journal papers indexed on Web of Science.
Desirable Criteria Experience in Raman spectroscopy. Experience of working with industry partners to solve industrially relevant materials-science orientated problems. Instrumentation -- Double aberration corrected, monochromatic FEI Titan Cubed Thermis 30 TEM with analytical facilities (SuperEDX and QuantumEELS), which will provide a unique capability in Ireland for ultra-high resolution imaging and spectroscopic analysis of materials. -- JEOL JEM-2100F with a field emission source, including bright-/dark field imaging, a STEM attachment, which simultaneously obtains bright field (BF) and high angle annular dark field (HAADF) images, an SEI/BSE detector, an energy dispersive X-ray analyser and computer controlled 3D reconstruction high angle tomography. -- JEOL JEM-2011 with a LaB6 source, STEM including bright-/dark field imaging, an energy dispersive X-ray analyser. --Hitachi SU-70 scanning electron microscope with a thermal field emission source, back scattered electron (BSE) detector, energy dispersive X-ray spectroscopy (EDS), wavelength dispersive X-ray spectroscopy (WDS) and an electron back scattered diffraction detector (EBSD); a coupled SEM-Raman system for simultaneous chemical characterisation coupled with high resolution imaging Login Host: 89.101.237.138 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: csuparna1-at-gmail.com Name: Suparna
Organization: UTHealth Medical School-Houston
Title-Subject: [Filtered] Site specific protein labeling
Message: Hi All,
Could anyone suggest me what would the best feasible way to label my membrane bound protein of interest in cell (facing towards the cytosol) to study its orientation and/or interaction with membrane using single molecule techniques like FRET/ FCCS/FLIM-FRET, etc. I am particularly interested to study the orientation of a specific domain of the protein with respect to the membrane in live cell.
} From literatures, I have learnt of applying specific ligand (bound to fluorophore) that would bind to the active site; but that might not work for my case as the protein domain is surface exposed and we don't know of any such ligands binding to that surface. Also, is there any hope of using a small peptide directed to the protein domain (or, the amino acid stretch) bound to fluorophore for this purpose?
Any help will be highly appreciated.
Thanks so much!
Suparna
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Message: The EMAS 2017 15th European Workshop and IUMAS-7 Meeting in Konstanz, Germany. [ 07.05.2017 until 11.05.2017] The primary aim of this combined EMAS Workshop / IUMAS Meeting is to assess the state of the art and reliability of microbeam analysis techniques. EMAS was founded in 1987 to meet the growing demands of microanalysis users and scientists across Europe for further education, communication and professional advice. IUMAS was founded in 1994 to promote world-wide cooperation in all aspects of microbeam analysis through the organisation of an international meeting on microbeam analysis every three or four years, and by participating in joint committees with other scientific organisations in matters relevant to microbeam analysis which are better discussed on a world scale.
The format of the meeting is aimed at maximising transfer of knowledge among the participants and at providing a comprehensive exhibition of the latest analytical equipment. The programme allows for adequate time and opportunities for participants to visit the technical exhibitions and hold discussions with the manufacturers.
The main topics of this, the 15th EMAS European Workshop, are: Pushing the Limits - EPMA; Modelling; Detector Technologies; Surface Characterisation; Cathodoluminescence; Pushing the Limits - General. Time will also be devoted to problem orientated applications in material science, geological science, environmental studies, astrophysics, microelectronics, forensics, cultural heritage and archaeology, nanomaterials, surfaces and interfaces, catalysts, sensors, ...
For details please go to the WWW site. https://www.microbeamanalysis.eu/events/event/24-emas-2017-15th-european-workshop-on-modern-developments-and-applications-in-microbeam-analysis-and-iumas-7-meeting
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you are putting the bar very high. Considering cell membranes are dynamic and EM techniques require cells to be killed, don't expect it to be an easy task. I would say the first consideration is the distance between your protein domain of interest and the cell membrane. There is a chance that this distance is too short to be resolvable by classical fluorescence microscopy techniques. Thanks God Stefan Hell came on earth to save fluorescence microscopists and now we have fluorescence microscopes with very high resolution, however there is not always one nearby. You are talking about FRET, meaning you probably expect distances of several nm. In this case, I don't see which technique would allow you to resolve this distance in live cells. Sure you'll see a fluorescence due to FRET but you won't be able to locate it with respect to the membrane, I am afraid. Please also consider the optical thickness of the plane observed in LM. I am not really up-to-date in confocal microscopy but I think you can at best get optical sections of 100nm, so you are limited in resolution in the Z axis as well. If distance is not such a big challenge than you can perhaps consider expressing your protein/domain with a fluorescent tag, like GFP or RFP?
Regards, Stephane
-------------------------------------------- On Mon, 10/17/16, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
Subject: [Microscopy] viaWWW:Site specific protein labeling To: nizets2-at-yahoo.com Date: Monday, October 17, 2016, 2:37 PM
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Email: csuparna1-at-gmail.com Name: Suparna
Organization: UTHealth Medical School-Houston
Title-Subject: [Filtered] Site specific protein labeling
Message: Hi All,
Could anyone suggest me what would the best feasible way to label my membrane bound protein of interest in cell (facing towards the cytosol) to study its orientation and/or interaction with membrane using single molecule techniques like FRET/ FCCS/FLIM-FRET, etc. I am particularly interested to study the orientation of a specific domain of the protein with respect to the membrane in live cell.
} From literatures, I have learnt of applying specific ligand (bound to fluorophore) that would bind to the active site; but that might not work for my case as the protein domain is surface exposed and we don't know of any such ligands binding to that surface. Also, is there any hope of using a small peptide directed to the protein domain (or, the amino acid stretch) bound to fluorophore for this purpose?
Any help will be highly appreciated.
Thanks so much!
Suparna
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Email: zhiping_luo-at-hotmail.com Name: Zhiping Luo
Organization: Fayetteville State University
Title-Subject: [Filtered] Two faculty positions in Physics/Materials Science available at Fayetteville State University
Message: There are two faculty position openings in Physics/Materials Science at Fayetteville State University:
Assistant/Associate Professor of Physics https://jobs.uncfsu.edu/postings/14519
Assistant/Associate Professor of Physical Science https://jobs.uncfsu.edu/postings/14513 FSU houses a field-emission EPMA. Candidates with expertise in EPMA are encouraged to apply.
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==============================Original Headers============================== 16, 53 -- From microscopy.listserver-at-gmail.com Wed Oct 19 07:33:54 2016 16, 53 -- Received: from mail-it0-f51.google.com (mail-it0-f51.google.com [209.85.214.51]) 16, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9JCXsmq007062 16, 53 -- for {microscopy-at-microscopy.com} ; Wed, 19 Oct 2016 07:33:54 -0500 16, 53 -- Received: by mail-it0-f51.google.com with SMTP id 4so110726880itv.0 16, 53 -- for {microscopy-at-microscopy.com} ; Wed, 19 Oct 2016 05:36:43 -0700 (PDT) 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 53 -- d=gmail.com; s=20120113; 16, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 53 -- :mime-version:in-reply-to:content-transfer-encoding; 16, 53 -- bh=sj6y15HKuCYAhKVUzGC8AHrwakhqzXCRweiWcVrTBLc=; 16, 53 -- b=RX4HslmuXotPkZ7LnNWU1Hezy/gyR+D1fCRdrQC0A2zmy64WHMCAuUaw3QVxrjHrVd 16, 53 -- A9rC0JoD8lRglsEbDX4kx7AsQy36L0FNZKjhO2vntwoy6mpKYdlUf80thqTF6neYYup/ 16, 53 -- W8Fhc6X80lAj8DX1zrJeS1cPp05ARlG3F5AlqUjDnzNcSNxR/TFVlcNJFyq4DWHDz6Eq 16, 53 -- Osmx0J9RuNr1k5sMsueuyd4DuBcgYHazEtxK2VMYjCbbM/mFE/CiVqWY6xYfI1dzDr54 16, 53 -- 7cy/A5mJE1OC2+Wksy1IsTR+5VvpEwPN2jzwjm80VeEnyiSgOi6JD95zihpYlxapZXEj 16, 53 -- dhuA== 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 53 -- d=1e100.net; s=20130820; 16, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 16, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 16, 53 -- bh=sj6y15HKuCYAhKVUzGC8AHrwakhqzXCRweiWcVrTBLc=; 16, 53 -- b=TBL55iJGFyT7lvuBajmIraoegh4tyra+uFK86VidP1+9FIiqeYC6DQBXMUz77gn0Uy 16, 53 -- 8bilVhe0v9qgIuEVxGAtkfvQUbuSriScaTAN9PwNxiYznfgbos8eAZqF3jciywAc6jRT 16, 53 -- rDLHgZEcihPW5AFCcGaBqY2taBoVFFrz4dXqeDJOf6cRGMVpDHTIEuY5C7p5nTW2LJ6s 16, 53 -- e5q3TudsvBmRY0cOpx5xK/aWHd39P6P0EMtyRZziF8Ztv/Y7Jct/yKDvxs+GCqQE10bX 16, 53 -- +ItfI/iLhVxjztrxFJ28SmvJv6g971qP69tRwDFilDlXKUButz4JCETpq447v8tdZVQB 16, 53 -- xNHA== 16, 53 -- X-Gm-Message-State: AA6/9RnL3UimlIM/b+2oXv4OwzDrNhhXD0bHQpdZM3uTNt3le0igpsF5yPoKzbc5VUSwMw== 16, 53 -- X-Received: by 10.36.156.197 with SMTP id b188mr2780794ite.28.1476880602793; 16, 53 -- Wed, 19 Oct 2016 05:36:42 -0700 (PDT) 16, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([24.15.143.135]) 16, 53 -- by smtp.googlemail.com with ESMTPSA id g76sm17564415iod.20.2016.10.19.05.36.42 16, 53 -- for {microscopy-at-microscopy.com} 16, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 53 -- Wed, 19 Oct 2016 05:36:42 -0700 (PDT) 16, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 53 -- {microscopylistserver-noreply-at-microscopy.com} 16, 53 -- Subject: viaWWW:Two faculty positions in Physics/Materials Science available 16, 53 -- at Fayetteville State University 16, 53 -- References: {201610182114.u9ILEhrY025551-at-ns.microscopy.com} 16, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 53 -- X-Forwarded-Message-Id: {201610182114.u9ILEhrY025551-at-ns.microscopy.com} 16, 53 -- Message-ID: {3434681d-2184-5749-3f80-d8d0ec8bf6cd-at-microscopy.com} 16, 53 -- Date: Wed, 19 Oct 2016 07:36:41 -0500 16, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 16, 53 -- Gecko/20100101 Thunderbird/45.4.0 16, 53 -- MIME-Version: 1.0 16, 53 -- In-Reply-To: {201610182114.u9ILEhrY025551-at-ns.microscopy.com} 16, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello Everyone, Please forward this note on other listservers that focus on microscopy or aspects of microscopy. I want to reach every member of Linked-In's Microscopy Group (over 5000!) so they have an opportunity to participate in the direction the group is moving. Not everyone checks their Linked-In daily.
The Microscopy Group was formed to connect anyone associated with a microscope with the job market. The primary function of the Microscopy Group is to help find jobs. Transfer of techniques or other information is a desirable, but accidental occurrence. This is your chance to change that. To accomplish this I allowed headhunters and recruiters to join, as well as anyone with a microscopy connection no matter how minor.
My only requirement is they type a sentence mentioning microscopy, microscope or any of the many techniques that enrich our field. Clicking a box claiming a skill doesn't make the grade.
I started the group, and it's my rules, but I'm retiring, so it could be your rules. Want to be group manager? Contact me and I'll set it up. What do you think?
Frank Karl Microscopy Group Manager Searcher12-at-gmail.com Or Frank_karl-at-ardl.com
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 9, 27 -- From frank_karl-at-ardl.com Wed Oct 19 09:37:55 2016 9, 27 -- Received: from cal1-mh675b.smtproutes.com (cal1-mh675b.smtproutes.com [208.70.89.128]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9JEbtKl002677 9, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Oct 2016 09:37:55 -0500 9, 27 -- X-Katharion-ID: 1476888035.79777.cal1-mh675 9, 27 -- Received: from mail.ardl.com ([74.207.215.218]) by 9, 27 -- cal1-mh675.smtpout.com [(192.69.16.47)] with ESMTP via TCP; 19 Oct 2016 9, 27 -- 14:40:35 +0000 9, 27 -- Received: from exchange2k7.ad.ardl.com ([fe80::d57a:b38c:8f17:9f73]) by 9, 27 -- exchange2k7.ad.ardl.com ([fe80::d57a:b38c:8f17:9f73%15]) with mapi; Wed, 19 9, 27 -- Oct 2016 10:37:07 -0400 9, 27 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 27 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 9, 27 -- Date: Wed, 19 Oct 2016 10:37:06 -0400 9, 27 -- Subject: change in Link-in Microscopy Group 9, 27 -- Thread-Topic: change in Link-in Microscopy Group 9, 27 -- Thread-Index: AdIqFkQeuOJR4OxPTKW0pSHR6djKZA== 9, 27 -- Message-ID: {0445DA8FFC235F4CBDAF9BACCF79E1212CB9CB4AA1-at-exchange2k7.ad.ardl.com} 9, 27 -- Accept-Language: en-US 9, 27 -- Content-Language: en-US 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- acceptlanguage: en-US 9, 27 -- Content-Type: text/plain; charset="us-ascii" 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9JEbtKl002677 ==============================End of - Headers==============================
} On Oct 19, 2016, at 7:51 AM, frank_karl-at-ardl.com wrote: } } } Hello Everyone, } Please forward this note on other listservers that focus on microscopy or aspects of microscopy. I want to reach every member of Linked-In's Microscopy Group (over 5000!) so they have an opportunity to participate in the direction the group is moving. Not everyone checks their Linked-In daily. } } The Microscopy Group was formed to connect anyone associated with a microscope with the job market. The primary function of the Microscopy Group is to help find jobs. Transfer of techniques or other information is a desirable, but accidental occurrence. This is your chance to change that. } To accomplish this I allowed headhunters and recruiters to join, as well as anyone with a microscopy connection no matter how minor. } } My only requirement is they type a sentence mentioning microscopy, microscope or any of the many techniques that enrich our field. Clicking a box claiming a skill doesn't make the grade. } } I started the group, and it's my rules, but I'm retiring, so it could be your rules. Want to be group manager? Contact me and I'll set it up. } What do you think? } } Frank Karl } Microscopy Group Manager
Dear Frank, I think this makes sense. The listservers for microscopy, 3dem, mod, etc. are good places for technique-related posts, so using LinkedIns group for job-related posts makes a good division of effort. Yours, Bill
==============================Original Headers============================== 7, 34 -- From wtivol-at-sbcglobal.net Wed Oct 19 19:06:42 2016 7, 34 -- Received: from nm25-vm10.access.bullet.mail.bf1.yahoo.com (nm25-vm10.access.bullet.mail.bf1.yahoo.com [216.109.115.201]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9K06fbU019936 7, 34 -- for {microscopy-at-microscopy.com} ; Wed, 19 Oct 2016 19:06:41 -0500 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1476922172; bh=cxP90zGZLcZkYPWHzhpz/oukHSS6nIjjHuFzXxLIeIs=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=YapPJHDeUN31rcZyjryTD+6cPvXBfXlx58vkDxxZu+izo0ZmlvofbuJi17oHwaqxKxbMG3+pqg7X2a5r5km4zeAEIegbBeW8OHJ2mkqshtPXLkcANWAf66axlAoUIP7QHi8ccaxH+CIJdCgp106W1tIGkkCCEQ8ZsutjIGJsU7CAqRChEP1XIlP1oLig9aVSYkhq7vZVZUulZk1tKtZ6x78fG9YcNjDw1mPrUXyKZX862nvYrWtXjTClUe5AmlHktgNeTsN0Jukl4yOrirancBnGir5QkA/ji7wMfVzMys+082zkf4xT8OdgK27+zoWp8GXUPhSisOR1bFYodigN5Q== 7, 34 -- Received: from [66.196.81.157] by nm25.access.bullet.mail.bf1.yahoo.com with NNFMP; 20 Oct 2016 00:09:32 -0000 7, 34 -- Received: from [98.139.244.50] by tm3.access.bullet.mail.bf1.yahoo.com with NNFMP; 20 Oct 2016 00:09:32 -0000 7, 34 -- Received: from [127.0.0.1] by smtp112.sbc.mail.bf1.yahoo.com with NNFMP; 20 Oct 2016 00:09:32 -0000 7, 34 -- X-Yahoo-Newman-Id: 923.20499.bm-at-smtp112.sbc.mail.bf1.yahoo.com 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-YMail-OSG: 3l_yvkwVM1nHs2GQMpSgW6p24eTyEMTb4z8eydyodUZGE_i 7, 34 -- DtmDH7NHg7rYtwGpoEEy3E_dIWKYfErNqjJNOPlz636kZzz4JGe.6IgiAhXi 7, 34 -- R01p5KSpm712Nqi06r2m.tA1OxZvTfCFstB.kbYsK4KTJTHAK3JiuVl1LhSY 7, 34 -- 7Q_VKYmFxOU7XtljAK6Yx3LF9MzlUsy3JYj4Ui.3GCsg5Z.26OPFdUsLyB7Z 7, 34 -- 8llaH8tFUJ88ludw9qa4k4aEA7ZjDChTnWlD2AjhethNBsjiHipNUSHtMita 7, 34 -- ix07H3eC.OaQowqaLVrAwJ9TK.V3aYMzn4ro4jyYuiLGEoOirTNbUZG2QZmB 7, 34 -- _iU8mxf5kCJdc2C6pKN_iW6rXeOjO2cMdT2_26ZJdAkb8oBFX8iAYAoQ957P 7, 34 -- 8I3zzElqAGDYcfRaaKE58qp.JZQKqJ2Wxfw.VMbjlWnjrc4Dq4cDLHXKb6mI 7, 34 -- lkEJ83Khqw.hKevSJ9hOlHVOYtEk15e2NGCBtdwEBBEnaYBh12ZOoQdaU7PD 7, 34 -- tl.21ikvwLEMLon60_198Vn1c5UaPDAk8o1DC1KVZ0NerxUgDpyzUN58eOCi 7, 34 -- mXERBm.A- 7, 34 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 7, 34 -- Content-Type: text/plain; charset=us-ascii 7, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 7, 34 -- Subject: Re: [Microscopy] change in Link-in Microscopy Group 7, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 7, 34 -- In-Reply-To: {201610191451.u9JEpTgL017117-at-ns.microscopy.com} 7, 34 -- Date: Wed, 19 Oct 2016 17:09:30 -0700 7, 34 -- Message-Id: {086A440C-5CC8-48B9-A9A8-9B26ED97F794-at-sbcglobal.net} 7, 34 -- References: {201610191451.u9JEpTgL017117-at-ns.microscopy.com} 7, 34 -- To: Frank Karl {frank_karl-at-ardl.com} , microscopy-at-microscopy.com 7, 34 -- X-Mailer: Apple Mail (2.3124) 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9K06fbU019936 ==============================End of - Headers==============================
Good Morning/Good Day/Good afternoon Dear 'Listers' Dear Colleagues,
allow me to send this message for your kind information (knowing not all MSA-Listserver members might have interest in dermatological / dermatopathological matters / structural and molecular Skin research - so -please- be patient]:
SAVE THE DATE *1st Announcement* Society for Cutaneous Ultrastructure Research NEXT MEETING of SCUR - The SKIN IMAGING SOCIETY -
44th ANNUAL MEETING: 8-10th June, 2017 MILANO, ITALY General Theme: " INSIDE OUT - OUTSIDE IN THE SKIN "
You are kindly invited to think about sending your abstract, or - most welcome - plan to attend the meeting. [Travel grants - Awards for best Oral - best Poster Presentation]
Further (preliminary) information at: www.scur.org NB: UPDATE & RELAUNCHING SCUR Website to be expected in November 2016
If you have further request for information - please - contact the Local Organizer via e-mail: scurmilan2017-at-gmail.com
Best regards and with my compliments
Wolfgang Muss, Ph.D., FRMS SCUR-BOARD MEMBER Emeritus member of the MSA ---------------------------------------------------------------------------- ---
AVISO for OCTOBER 2018 - SAVE THE DATEs - : 7th Joint Meeting of the Society for Skin Structure Research (SSSR, Japan) with SCUR - The Skin Imaging Society
The (Japanese) SOCIETY for SKIN STRUCTURE RESEARCH (SSSR) AND The Society for Cutaneous Ultrastructure Research (SCUR) - The SKIN IMAGING SOCIETY proudly announce their 7th Joint Meeting (= also 45th Annual Meeting of SCUR)
to be held October 3rd (half day), 4th (full day), 5th (half day), 2018
VENUE: Asahikawa Grand Hotel, Asahikawa, Japan.
This Joint Meeting takes place prior to The 82nd Annual Meeting of the Eastern Division of Japanese Dermatology Association (October 6th to 7th, 2018, same location).
==============================Original Headers============================== 18, 20 -- From wij.muss-at-aon.at Thu Oct 20 04:46:19 2016 18, 20 -- Received: from bsmtp.bon.at (bsmtp.bon.at [213.33.87.14]) 18, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9K9kI8S001929 18, 20 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2016 04:46:19 -0500 18, 20 -- Received: from MussTHINK (unknown [93.83.25.98]) 18, 20 -- by bsmtp.bon.at (Postfix) with ESMTPSA id 3t03tF5QfXz5tm2; 18, 20 -- Thu, 20 Oct 2016 11:49:09 +0200 (CEST) 18, 20 -- From: "MUSS Wolfgang \(SCUR Board Member\)" {wij.muss-at-aon.at} 18, 20 -- To: {microscopy-at-microscopy.com} 18, 20 -- Subject: Announcement for June 2017 - SAVE the DATE 44th Ann. Meeting of SCUR - The Skin Imaging Society (Society for Cutaneous Ultrastructure Research) AND AVISO for SSSR-SCUR Joint Meeting Oct. 2018 18, 20 -- Date: Thu, 20 Oct 2016 11:49:09 +0200 18, 20 -- Message-ID: {005501d22ab7$34bf4de0$9e3de9a0$-at-aon.at} 18, 20 -- MIME-Version: 1.0 18, 20 -- Content-Type: text/plain; 18, 20 -- charset="iso-8859-1" 18, 20 -- X-Mailer: Microsoft Outlook 15.0 18, 20 -- Content-language: de 18, 20 -- Thread-Index: AdIqthARUajMZjM/Sv2j49u8rnpCig== 18, 20 -- Content-Transfer-Encoding: 8bit 18, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9K9kI8S001929 ==============================End of - Headers==============================
It's a good idea to post jobs on Linked-In, but I strongly urge people to continue posting job openings on the MSA web site - under "Resources" - and here on the list server.
Linked-In is a social media member site that requires registering and providing personal information which is then used for marketing by Linked-In. Basically, another kind of Facebook (if Facebook doesn't already own Linked-In - do they?). With all the privacy caveats one has with Facebook and the like.
Why I don't belong. That, and getting numerous requests from other people I don't know "inviting" me to join Linked-In, and who frequently have nothing to do with microscopy, based on the information provided in the invitation. I wonder if the people from whom I get these invites even know they're being sent to me?
One should think harder about joining some social site without full knowledge of what that site does with your information and how they make their money. It can only be by selling your information - to corporations and groups you don't know who will use your information in ways you don't know and can't control.
Linked-In may be useful, but let's not require people to go there for their job searches.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 ________________________________________ X-from: wtivol-at-sbcglobal.net {wtivol-at-sbcglobal.net} Sent: Wednesday, October 19, 2016 20:22 To: Oshel, Philip Eugene
} On Oct 19, 2016, at 7:51 AM, frank_karl-at-ardl.com wrote: } } } Hello Everyone, } Please forward this note on other listservers that focus on microscopy or aspects of microscopy. I want to reach every member of Linked-In's Microscopy Group (over 5000!) so they have an opportunity to participate in the direction the group is moving. Not everyone checks their Linked-In daily. } } The Microscopy Group was formed to connect anyone associated with a microscope with the job market. The primary function of the Microscopy Group is to help find jobs. Transfer of techniques or other information is a desirable, but accidental occurrence. This is your chance to change that. } To accomplish this I allowed headhunters and recruiters to join, as well as anyone with a microscopy connection no matter how minor. } } My only requirement is they type a sentence mentioning microscopy, microscope or any of the many techniques that enrich our field. Clicking a box claiming a skill doesn't make the grade. } } I started the group, and it's my rules, but I'm retiring, so it could be your rules. Want to be group manager? Contact me and I'll set it up. } What do you think? } } Frank Karl } Microscopy Group Manager
Dear Frank, I think this makes sense. The listservers for microscopy, 3dem, mod, etc. are good places for technique-related posts, so using LinkedIns group for job-related posts makes a good division of effort. Yours, Bill
==============================Original Headers============================== 14, 71 -- From oshel1pe-at-cmich.edu Thu Oct 20 07:32:15 2016 14, 71 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 14, 71 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9KCWFMV032342 14, 71 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2016 07:32:15 -0500 14, 71 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 14, 71 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u9KCZ6xb029448 14, 71 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2016 08:35:06 -0400 14, 71 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 14, 71 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 14, 71 -- (TLS) id 14.3.248.2; Thu, 20 Oct 2016 08:35:06 -0400 14, 71 -- Received: from NAM02-SN1-obe.outbound.protection.outlook.com (141.209.2.100) 14, 71 -- by mail.cmich.edu (141.209.15.40) with Microsoft SMTP Server (TLS) id 14, 71 -- 14.3.248.2; Thu, 20 Oct 2016 08:35:05 -0400 14, 71 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 71 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; 14, 71 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 14, 71 -- bh=nQUucD29IgCVN4Jvc0NovWS/sP9HxZTVm4e59xpwS8I=; 14, 71 -- b=QlWhtmv0tsevE/hmtAfij82dyN6kbvTGLR0aA/55Zt6oTwtjOk9I/KJCu8g0BWxyYv0sKWBdJks06mCuyXtGs5PsjLNdVcVtHhJSSpIpxpt1Pv3GF8lQ090BR2EIIChngJpzOvibHsqI74KcETgD3XEirWE7JpprVfd8qvJ5ZFY= 14, 71 -- Received: from CY1PR05MB1996.namprd05.prod.outlook.com (10.162.216.30) by 14, 71 -- CY1PR05MB1994.namprd05.prod.outlook.com (10.162.216.28) with Microsoft SMTP 14, 71 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 14, 71 -- 15.1.669.5; Thu, 20 Oct 2016 12:35:04 +0000 14, 71 -- Received: from CY1PR05MB1996.namprd05.prod.outlook.com ([10.162.216.30]) by 14, 71 -- CY1PR05MB1996.namprd05.prod.outlook.com ([10.162.216.30]) with mapi id 14, 71 -- 15.01.0669.018; Thu, 20 Oct 2016 12:35:04 +0000 14, 71 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 14, 71 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 71 -- Subject: Re: [Microscopy] Re: change in Link-in Microscopy Group 14, 71 -- Thread-Topic: [Microscopy] Re: change in Link-in Microscopy Group 14, 71 -- Thread-Index: AQHSKmiDExllzc0hzUGM/MMO8AyJiqCxRLzE 14, 71 -- Date: Thu, 20 Oct 2016 12:35:04 +0000 14, 71 -- Message-ID: {CY1PR05MB19960C1332F724E0B49235AB95D50-at-CY1PR05MB1996.namprd05.prod.outlook.com} 14, 71 -- References: {201610200022.u9K0MuAF004297-at-ns.microscopy.com} 14, 71 -- In-Reply-To: {201610200022.u9K0MuAF004297-at-ns.microscopy.com} 14, 71 -- Accept-Language: en-US 14, 71 -- Content-Language: en-US 14, 71 -- X-MS-Has-Attach: 14, 71 -- X-MS-TNEF-Correlator: 14, 71 -- authentication-results: spf=none (sender IP is ) 14, 71 -- smtp.mailfrom=oshel1pe-at-cmich.edu; 14, 71 -- x-ms-exchange-messagesentrepresentingtype: 1 14, 71 -- x-originating-ip: [141.209.160.221] 14, 71 -- x-ms-office365-filtering-correlation-id: 10d5075d-d506-493f-6f88-08d3f8e584ae 14, 71 -- x-microsoft-exchange-diagnostics: 14, 71 -- 1;CY1PR05MB1994;6:SaQ+QrU9wQOeIqAKrKEzYkxIHzD9cXvD7ADv9Eb8nR+477QF19Tbb3270HGYpUy4JPwuIeAXwLNzOa+A4DubU7P1VOZAbXA7pMS3xph+/LsO/kUYfYJxHKqFa+pARI0HJmd4WdqN1v0KFkbt8US299N+UAnzDo57uvcSEpAOLIPuQFXn4Xj6XUTiPW/b9TzGe+yQDSYMXgMPEdZg6w3rCcBMcWrCfuuoVt8vJYJ1v0Lh4+hJvRxxX2S5WjSDYVa3Xf1/UJrIljcrx56G+I/Qu5eXk/dF35CuD0fbzmugKTLGcQ7fCnPQIGnou4UU7ixi;5:2LFbUs/Yj0EMCE2EuIAICbl4AWGur7CLH1tLQew3P/fmZoVPEJPX4XPSOGAwKmZkvgN3ASikucLD6zLc6iQm6qVcDy40XFt7HCLDLeZDXp3yYa8NJBg4liPeplYQY7CE5CkpTI3GMHhlKg2TgQxWB7tEV/+wDxsXPsvQdWppKDs=;24:eIXn/aCXAHFTMZArBBG37NBse7+Pa8hgKO2Yi2Ymdl8SmO37LKxamZ89Zs2moa3yP0O8hRO7ddSfZ1IwusDhqYCOGG4/5rSAXB17oVYhFBk=;7:dYCh8jYDMa/C29iwyr+ZMGAjXRNKv26ozrnVG882S6b81kRhxwd3PDh7UPFL+Hpy0/PikCzDXFy47Z7lHbv0TuU1T71scZlpmP8pHsPfs39+jEETdLeBThDcAvEoof8It0aoOhJqVHRDZrt4pRGqXLEGCK2sXVYrRJPYRPBhY2fzt09Sy2pXG9sqFkHJ7YHMow1Yj0kw1Bvb4J0EsjpD8ZNYvAarLRMSf3mP+27vowSd5nDT3DrfFTwx09OpYCIgOnGUEfTcKoIWzHTRClz5dr2M3oz2r/oGqekVuavl6ygU0qqKckW6Kv9eu0uEriZs9jRFjQ0dyXaRcGq4/lZ/Oq+MDhHAHyHp14mOrPpf! 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} } Phil; } Microsoft bought LinkedIn for $26Bn to make money through the devious methods you describe. } } A. John Mardinly, Ph.D., P.E. } Retired } } } } } } }
==============================Original Headers============================== 3, 38 -- From John.Mardinly-at-asu.edu Thu Oct 20 18:13:31 2016 3, 38 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 3, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u9KNDVTW017175 3, 38 -- for {Microscopy-at-Microscopy.com} ; Thu, 20 Oct 2016 18:13:31 -0500 3, 38 -- X-ASG-Debug-ID: 1477005372-06a8672d0936d10002-FOsErg 3, 38 -- Received: from exhubw02.asurite.ad.asu.edu (exhubw02.asurite.ad.asu.edu [129.219.4.200]) by bcnet8.asu.edu with ESMTP id 4GK3GgoO7cEQXPZe (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Thu, 20 Oct 2016 16:16:15 -0700 (MST) 3, 38 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 3, 38 -- X-Barracuda-Effective-Source-IP: exhubw02.asurite.ad.asu.edu[129.219.4.200] 3, 38 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 3, 38 -- X-ASG-Whitelist: Client 3, 38 -- Received: from EXMBW01.asurite.ad.asu.edu ([169.254.8.50]) by 3, 38 -- exhubw02.asurite.ad.asu.edu ([129.219.4.200]) with mapi id 14.03.0266.001; 3, 38 -- Thu, 20 Oct 2016 16:16:13 -0700 3, 38 -- From: John Mardinly {John.Mardinly-at-asu.edu} 3, 38 -- To: MSA {Microscopy-at-Microscopy.com} 3, 38 -- Subject: Fwd: [Microscopy] change in Link-in Microscopy Group 3, 38 -- Thread-Topic: [Microscopy] change in Link-in Microscopy Group 3, 38 -- X-ASG-Orig-Subj: Fwd: [Microscopy] change in Link-in Microscopy Group 3, 38 -- Thread-Index: AQHSKtBiXOCwZXpifUKPi5a4x7bYYaCyBkwA//+LYqmAAGiXzw== 3, 38 -- Date: Thu, 20 Oct 2016 23:16:13 +0000 3, 38 -- Message-ID: {56AC4C46-1A87-4B50-A8F7-1B50838C849F-at-asu.edu} 3, 38 -- References: {201610201246.u9KCkQQa012894-at-ns.microscopy.com} , {E91FE88F-6F09-4BAA-ABD8-B7445A705D1E-at-asu.edu} , {CY1PR05MB19963831EAC04AA0985A2DFF95D50-at-CY1PR05MB1996.namprd05.prod.outlook.com} 3, 38 -- In-Reply-To: {CY1PR05MB19963831EAC04AA0985A2DFF95D50-at-CY1PR05MB1996.namprd05.prod.outlook.com} 3, 38 -- Accept-Language: en-US 3, 38 -- Content-Language: en-US 3, 38 -- X-MS-Has-Attach: 3, 38 -- X-MS-TNEF-Correlator: 3, 38 -- Content-Type: text/plain; charset="us-ascii" 3, 38 -- MIME-Version: 1.0 3, 38 -- X-Barracuda-Connect: exhubw02.asurite.ad.asu.edu[129.219.4.200] 3, 38 -- X-Barracuda-Start-Time: 1477005375 3, 38 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA 3, 38 -- X-Barracuda-URL: https://129.219.117.210:443/cgi-mod/mark.cgi 3, 38 -- X-Barracuda-Scan-Msg-Size: 187 3, 38 -- X-Virus-Scanned: by bsmtpd at asu.edu 3, 38 -- X-Barracuda-BRTS-Status: 1 3, 38 -- Content-Transfer-Encoding: 8bit 3, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9KNDVTW017175 ==============================End of - Headers==============================
Can someone unconfuse me about how LR White is supplied and used?
I am getting confused by the labels that say ‘catalyzed’ and when to use the separate ‘accelerator’
Is the benzoyl peroxide accelerator only added for doing cold cures?
What is the catalyst that is already mixed in? Does this affect the shelf life or heat cure?
I thought I had this figured out but this morning a student brought me a vial of LR White that had polymerized over night in the refrigerator. No accelerator added, straight from the stock bottle. Can’t figure that one out.
I am now reconfused about this stuff and need some remedial education.
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 10, 40 -- From prvs=10959677f8=jkrupp-at-deltacollege.edu Fri Oct 21 11:00:40 2016 10, 40 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 10, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u9LG0e3s016797 10, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2016 11:00:40 -0500 10, 40 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 10, 40 -- by localhost (Email Security Appliance) with SMTP id 8A6C217553C_80A3C58B 10, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2016 16:03:36 +0000 (GMT) 10, 40 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 10, 40 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 40 -- (Client did not present a certificate) 10, 40 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTPS id 707AB17373C_80A3C58F 10, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2016 16:03:36 +0000 (GMT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5125C6D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2016 09:02:51 -0700 (PDT) 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 10, 40 -- with ESMTP id qgEqf1RDS5BE for {Microscopy-at-microscopy.com} ; 10, 40 -- Fri, 21 Oct 2016 09:02:51 -0700 (PDT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id EC1F36D1E743 10, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2016 09:02:50 -0700 (PDT) 10, 40 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 10, 40 -- with ESMTP id 6nIPo8w-VDNf for {Microscopy-at-microscopy.com} ; 10, 40 -- Fri, 21 Oct 2016 09:02:50 -0700 (PDT) 10, 40 -- Received: from [10.246.130.205] (unknown [10.246.130.205]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id CC0916D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2016 09:02:50 -0700 (PDT) 10, 40 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 10, 40 -- Content-Type: text/plain; charset=utf-8 10, 40 -- Subject: LR White 10, 40 -- Message-Id: {14604EB1-58B7-4424-9202-6F0C80D64B06-at-deltacollege.edu} 10, 40 -- Date: Fri, 21 Oct 2016 09:03:17 -0700 10, 40 -- To: Microscopy-at-microscopy.com 10, 40 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 10, 40 -- X-Mailer: Apple Mail (2.3124) 10, 40 -- Content-Transfer-Encoding: 8bit 10, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9LG0e3s016797 ==============================End of - Headers==============================
The Analytical Imaging Facility of Albert Einstein College of Medicine in New York City has an opening for an experienced electron microscopy technician.
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The successful candidate must be well versed in a variety of standard electron microscopic methods, with two to four years (or equivalent) of experience in electron microscopy. The technician must be proficient in sample preparation techniques for electron microscopy. The ability to operate transmission electron microscopes and scanning electron microscopes is essential. Have working ability in digital imaging and quantitative image analysis. An understanding of scientific literature and moderate understanding of research goals and rationale is required. It is essential that the technician have excellent communication skills. Finally, the technician must be able to work well with many people in a multi-user environment.
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Thank you everyone for help. The flashes we observed were indeed due to fluorescent screen charging, further action awaits administrative approval! For completion sake here is the summary: Problem: Bright flashes of light were observed with a click sound on flourescent screen. however on suggestion from listerver and further analysis:
1. No sound were heard when screen was "up" 2. No change in intensity seen on CCD camera 3. FE electron gun showed no change in intensity (looking through the window on electron gun) 4. No accelerating voltage fluctuation 5. No vacuum pressure level fluctuation of any kind 6. Flashed were more prominent during alignment (when image intensity will change a lot and screen will be thoroughly radiated due to various intensity wobbling etc.) 7. proper corona discharge kind of flash was also seen once on twice (at the edge of screen) 8. Issue disappeared on own for few weeks, now surfaced again
Current reasoning: In JEOL JEM screen is held by 3 screws (as told by JEOL engineer) one of which is grounded, looks like ground screw is bit dodgy as the moment.
Will let everyone know in case any development occurs.
Thank you so much again everyone for help.
==============================Original Headers============================== 5, 37 -- From amit.welcomes.u-at-gmail.com Mon Oct 24 00:19:47 2016 5, 37 -- Received: from mail-yw0-f176.google.com (mail-yw0-f176.google.com [209.85.161.176]) 5, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9O5JlOi027374 5, 37 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2016 00:19:47 -0500 5, 37 -- Received: by mail-yw0-f176.google.com with SMTP id t192so170420235ywf.0 5, 37 -- for {microscopy-at-microscopy.com} ; Sun, 23 Oct 2016 22:22:53 -0700 (PDT) 5, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 37 -- d=gmail.com; s=20120113; 5, 37 -- h=mime-version:from:date:message-id:subject:to; 5, 37 -- bh=CdXLH27TdYLi/CW+szD3+NsV3CAMvO8u6DahbpJ8tWU=; 5, 37 -- b=xEPM+FkqNXLrppXEnyCUOTDA4QILNeYz76OB2C289xXtfoXYbyKpYC9f3qVoajbxo8 5, 37 -- /FmDeT/PZk5Npo1AUYrBThSC1XbGWx57+g5isSFbnxYBelZe4wJ3Y6donm3CmJjhCkKx 5, 37 -- ja3NDClKVuSQ3l8quRQVao6hlpsU+dN3NwXKUpFn2/LBDvlMW/oUPQjv3ZJoS1Rmk5B+ 5, 37 -- aVw/PTNxoEArsJbEyxcyxUMxGDT8YS/YALOtYL+Opdhyzr5452UmQh7erbBwoShWktnN 5, 37 -- FyBNWl8Er51S3nMkk3inAjb/oPivSdCYSbbhiKJGG7e5lLLGJwxtLgwlntf48TvSb0TQ 5, 37 -- WXyQ== 5, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 37 -- d=1e100.net; s=20130820; 5, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 5, 37 -- bh=CdXLH27TdYLi/CW+szD3+NsV3CAMvO8u6DahbpJ8tWU=; 5, 37 -- b=EBQ2g6bGEAtc593wu7Fp4D5gUrSlqT6IosUAwtrLxP4wzgr9YWbgtdIQUwt4vinLW2 5, 37 -- JAbUAMan1RRGXT15HfTUU8FRX2HT9I+8cRMWz/fyIVwiNhSdJ9awJq968ROFwKVWZ2hP 5, 37 -- b+OUKq9V7lTTcU3XUhFRDcEbJVgm38zbf2mkCfVStPvNP2F5+zd2jwNWavtFvlDnrNV9 5, 37 -- xehWjPXW2kTlzCorfcBUWs4fxJYGS5ByDlNdb0P8wbNnTaB/nnKE2eoWNRXHQzSEaNMj 5, 37 -- wQLGPUO7A2qZ88RKXb70SU8kQ0xQqqfByRXgTldGVOVm5+WfbS4flwI2RLitTMrypgsa 5, 37 -- JCVg== 5, 37 -- X-Gm-Message-State: ABUngvfRi6sSQQGzpg6kOPpJrBked6f7n3NOUVQVHsj6u9+hKLMkKfJFXMibO9xr2z5FwzfyhyUh/3c+cfzhtA== 5, 37 -- X-Received: by 10.13.217.194 with SMTP id b185mr13336477ywe.81.1477286572481; 5, 37 -- Sun, 23 Oct 2016 22:22:52 -0700 (PDT) 5, 37 -- MIME-Version: 1.0 5, 37 -- Received: by 10.37.18.138 with HTTP; Sun, 23 Oct 2016 22:22:32 -0700 (PDT) 5, 37 -- From: Amit Gupta {amit.welcomes.u-at-gmail.com} 5, 37 -- Date: Mon, 24 Oct 2016 10:52:32 +0530 5, 37 -- Message-ID: {CA+bx8_yPPXaAdfGFNq68_iDQPXizd9KjQSgyvuhGHN9ZzQqqpQ-at-mail.gmail.com} 5, 37 -- Subject: [Sum up] Sudden flashes seen on Fluorescent screen of TEM 5, 37 -- To: microscopy {microscopy-at-microscopy.com} 5, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Be sure to check out today's doodle at Google - it features van Leeuwenhoek
Thomas E. Phillips, Ph.D Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax)
==============================Original Headers============================== 4, 36 -- From PhillipsT-at-missouri.edu Mon Oct 24 08:45:05 2016 4, 36 -- Received: from mst-rip6-missouri-out.um.umsystem.edu (mst-rip6-missouri-out.um.umsystem.edu [198.209.50.149]) 4, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9ODj51T007692 4, 36 -- for {microscopy-at-microscopy.com} ; Mon, 24 Oct 2016 08:45:05 -0500 4, 36 -- X-IronPort-Anti-Spam-Filtered: true 4, 36 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2EgCwD+Dw5Y/9CeoM9ZAxwBAQQBAQoBA?= 4, 36 -- =?us-ascii?q?YMqAQEBAQEdSoESAac3kzYBhiACgWJDEAECAQEBAQEBAQNfKIRjAgQ6TQICASo?= 4, 36 -- =?us-ascii?q?UEBYcHwYCBBuISr5RAQaDLQEBAQEGAQEBAQEBAQEBHgWGOIZSgkwfJoJpgi8Fm?= 4, 36 -- =?us-ascii?q?hQBkWoBjimRBzUfLYUziDMBfwEBAQ?= 4, 36 -- X-IPAS-Result: =?us-ascii?q?A2EgCwD+Dw5Y/9CeoM9ZAxwBAQQBAQoBAYMqAQEBAQEdSoE?= 4, 36 -- =?us-ascii?q?SAac3kzYBhiACgWJDEAECAQEBAQEBAQNfKIRjAgQ6TQICASoUEBYcHwYCBBuIS?= 4, 36 -- =?us-ascii?q?r5RAQaDLQEBAQEGAQEBAQEBAQEBHgWGOIZSgkwfJoJpgi8FmhQBkWoBjimRBzU?= 4, 36 -- =?us-ascii?q?fLYUziDMBfwEBAQ?= 4, 36 -- Received: from um-ncas4.um.umsystem.edu ([207.160.158.208]) 4, 36 -- by mst-rip6-exch-relay.um.umsystem.edu with ESMTP; 24 Oct 2016 08:48:12 -0500 4, 36 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.20]) by 4, 36 -- UM-NCAS4.um.umsystem.edu ([207.160.158.208]) with mapi id 14.03.0266.001; 4, 36 -- Mon, 24 Oct 2016 08:48:11 -0500 4, 36 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 4, 36 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 4, 36 -- Subject: today's doodle 4, 36 -- Thread-Topic: today's doodle 4, 36 -- Thread-Index: AQHSLf0a19dG72M3D0+0BxUrIVuNMqC3nl7g 4, 36 -- Date: Mon, 24 Oct 2016 13:48:11 +0000 4, 36 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363DE7796ED8-at-UM-MBX-T02.um.umsystem.edu} 4, 36 -- References: {201610241343.u9ODhepV007618-at-ns.microscopy.com} 4, 36 -- In-Reply-To: {201610241343.u9ODhepV007618-at-ns.microscopy.com} 4, 36 -- Accept-Language: en-US 4, 36 -- Content-Language: en-US 4, 36 -- X-MS-Has-Attach: 4, 36 -- X-MS-TNEF-Correlator: 4, 36 -- x-originating-ip: [207.160.158.193] 4, 36 -- Content-Type: text/plain; charset="us-ascii" 4, 36 -- MIME-Version: 1.0 4, 36 -- Content-Transfer-Encoding: 8bit 4, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u9ODj51T007692 ==============================End of - Headers==============================
Hello, LR White, (London resin white) is used mainly for post-embed immuno- electron microscopy. The cut surface allows for epitopes to be exposed and labeled with antibodies without the need to etch or antigen retrieval techniques. Most times it contains the catalyst so polymerization can be done at 50 C. Check with the manufacturer but it can also be UV polymerized (you may need to add a different catalyst). Most samples are lightly fixed ( no high concentrations of glutaraldehyde) not osmicated and dehydrated up to 70% ETOH (I usually go to 90% as the 70% is sometimes difficult to completely mix with the resin). Uranyl acetate and small amounts of tannic acid may be used in lieu of osmium. Sections are collected on formvar grids as the resin is beam unstable. Semi thin sections can also be generated to try immunofluorescence experiments.
Good luck, Michael
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Friday, October 21, 2016 12:18 PM To: mdelann1-at-jhmi.edu
Can someone unconfuse me about how LR White is supplied and used?
I am getting confused by the labels that say b
==============================Original Headers============================== 11, 24 -- From prvs=0983e338e=mdelann1-at-jhmi.edu Mon Oct 24 10:37:48 2016 11, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.199.119]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9OFbmJK002256 11, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2016 10:37:48 -0500 11, 24 -- X-IronPort-AV: E=Sophos;i="5.31,542,1473134400"; 11, 24 -- d="scan'208";a="19233507" 11, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 11, 24 -- by IronEB1.johnshopkins.edu with ESMTP/TLS/DHE-RSA-AES256-SHA; 24 Oct 2016 11:40:54 -0400 11, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 11, 24 -- To: {jkrupp-at-deltacollege.edu} , {Microscopy-at-microscopy.com} , 11, 24 -- {microscopy-at-msa.microscopy.com} , 11, 24 -- {microscopylistserver-noreply-at-microscopy.com} 11, 24 -- References: {201610211617.u9LGHaFC031215-at-ns.microscopy.com} 11, 24 -- In-Reply-To: {201610211617.u9LGHaFC031215-at-ns.microscopy.com} 11, 24 -- Subject: RE: [Microscopy] LR White 11, 24 -- Date: Mon, 24 Oct 2016 11:40:55 -0400 11, 24 -- Message-ID: {006a01d22e0d$025bd130$07137390$-at-jhmi.edu} 11, 24 -- MIME-Version: 1.0 11, 24 -- Content-Type: text/plain; 11, 24 -- charset="us-ascii" 11, 24 -- Content-Transfer-Encoding: 7bit 11, 24 -- X-Mailer: Microsoft Outlook 14.0 11, 24 -- Thread-Index: AQEl5Q/pEsW+hcvtlK/33G12gKgBCKIQTbWw 11, 24 -- Content-Language: en-us ==============================End of - Headers==============================
Today is Antoni van Leeuwenhoeks 384th Birthday and Google is celebrating it with today's "Google Doodle". VIsit Google and enjoy is view of "the tiny world in a drop of water."
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What are YOUR thoughts on the Lab of the Future? Give us your input: http://microscopyeducation.com/current-study-lab-of-the-future/
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Email: Tong.Wang-at-asrc.cuny.edu Name: Tong Wang
Organization: Advanced Science Research Center, The City University of New York
Title-Subject: [Filtered] EM Specialist position at CUNY ASRC
Message: Dear Colleagues,
The Imaging Facility of Advanced Science Research Center at The City Univer= sity of New York is looking for an EM Specialist interested in managing the= Helios dualbeam FIB-SEM and Titan Themis (S)TEM. The facility houses a Tit= an Themis 200kV (S)TEM with a Cs image corrector and a superX EDX detector = and a Helios Nanolab 660 dualbeam FIB-SEM for characterization, sample prep= aration, and 3D visualization.
More information about this opening can be found here: https://home.cunyfirst.cuny.edu/psp/cnyepprd/GUEST/HRMS/c/HRS_HRAM.HRS_CE.G= BL?Page=3DHRS_CE_JOB_DTL&Action=3DA&JobOpeningId=3D15137&SiteId=3D1&Posting= Seq=3D1#15137 or http://cuny.jobs/new-york-ny/electron-microscopy-specialist/66DB9772EDAA4A7= 1A188C6167B6DBE20/job/
More information about the CUNY ASRC Imaging Facility can be found here: http://nanoscience.asrc.cuny.edu/facilities/imaging-suite/
Sincerely,
Tong Wang Imaging Facility Manager CUNY Advanced Science Research Center 85 Saint Nicholas Terrace New York, NY 10031 212-413-3352 Tong.Wang-at-asrc.cuny.edu
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Hello, ZS Genetics has an opening for a full-time electron microscopy scientist.
This position is in support of technology development for EM-based DNA sequencing and analysis. The microscopist will be responsible for imaging activities in aberration corrected Scanning Transmission Electron Microscopy (AC-STEM) for research and development activities surrounding heavy atom labeled DNA imaging. The microscopist will participate in design, implementation, and proving of novel capabilities on the instruments. The microscopist will participate in automation of imaging and data management on the instrument. This represents an opportunity to do applications development in microscopy in a commercial setting. Please see the full job description at http://www.zsgenetics.com/open-positions/. Qualified candidates can email their resume to the address listed on the web page or to myself.
Regards, Larry Scipioni ZS Genetics
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Email: steve.sem-at-icloud.com Name: Steve Perry
Title-Subject: [Filtered] Service Engineer for FEI Tecnai Message: I would like to hear from a service engineer who is capable and available to perform service work on our Tecnai F30.
Located on the West coast USA. Travel and accommodations can be provided if required.
Reply in confidence,
Steve Perry
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Email: Tong.Wang-at-asrc.cuny.edu Name: Tong Wang
Organization: Advanced Science Research Center, The City University of New York
Title-Subject: [Filtered] updates on "EM Specialist position at CUNY ASRC"
Message: Dear Colleagues,
The job links in previous message are too long to be displayed as one clickable link. Please use the following link and search for "Electron Microscopy Specialist": http://www.asrc.cuny.edu/faculty-opportunities/jobs/
sincerely,
Tong Wang
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Email: yliu78-at-ncsu.edu Name: Yang Liu
Organization: Analytical Instrumentation Facility (AIF), North Carolina State Univ
Title-Subject: [Filtered] Opening for Electron Microscopy staff or postdoc at AIF NCSU
Message: The Analytical Instrumentation Facility (AIF) seeks a talented and industrious Electron Microscopist to join our team as either a postdoc or a full-time staff member (as appropriate for their experience). Please encourage talented applicants to apply!: Full-time staff position: https://jobs.ncsu.edu/postings/76529 Postdoc position: https://jobs.ncsu.edu/postings/76522
The AIF is NC States primary shared facility for materials characterization with a mission to enable and lead state-of-the-art research through acquisition, development, maintenance, training, and access to major analytical and materials characterization instrumentation. Through the support of engaged faculty and experienced staff, the AIF supports state-of-the-art scanning and transmission electron microscopes, X-ray scattering and spectroscopy instruments, mass spectrometry, scanning probe microscopy, nanoindentation, and extensive sample preparation facilities. The AIF is a core nanotechnology user facility in the new Research Triangle Nanotechnology Network (RTNN), a site in the National Nanotechnology Coordinated Infrastructure (NNCI).
Primary responsibilities of the new position include training new users (both internal users from NC State and those external to NC State) as well as performing service for external clients. The ideal candidate will be customer-focused and exhibit a commitment to excellence in all technical and organizational aspects of their role. The new position will work closely with the AIF and RTNN teams in serving the needs of university, industrial, and government researchers from across NC State, the North Carolina Research Triangle, and the nation.
Questions about the position can be directed to aif-contact-at-ncsu.edu.
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Email: tzega-at-lpl.arizona.edu Name: Tom Zega
Organization: University of Arizona, Kuiper Electron Microscopy Facility
Title-Subject: [Filtered] Staff Scientist Position
Message: Dear Colleagues, The office of Research, Discovery, and Innovation (RDI) at the University of Arizona invites applications for an appointed staff scientist position at the Assistant, Associate or (full) Staff level, dependent on experience, for a soon-to-be installed state-of-the-art transmission electron microscope (TEM) laboratory. The TEM laboratory is part of RDI Imaging Cores at the University of Arizona that are dedicated to research, instructional, industrial, and clinical applications. Services in the electron microscopy facility include Scanning Electron Microscopy (SEM), Focused-Ion-Beam (FIB) microscopy, and TEM. The scientist will be responsible for user training, sample preparation, imaging, spectroscopic characterization, instrument maintenance, calibration, troubleshooting and repair, experimental design, and advanced image/data analysis and archiving for the TEM laboratory.
Information about this position and how to apply can be found here: https://uacareers.com/postings/14485
Sincerely,
Tom
------------------------------------------------------------ Thomas J. Zega, Ph.D. Associate Professor Lunar and Planetary Laboratory Dept. of Materials Science and Engineering University of Arizona 1629 E. University Blvd. Tucson AZ 85721-0092 (v) 520-626-1356 (f) 520-621-4933 https://www.lpl.arizona.edu/PMRG/ ------------------------------------------------------------
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Email: andrei.kolmakov-at-nist.gov Name: Andrei Kolmakov
Organization: CNST NIST
Title-Subject: [Filtered] postdoctoral position in EM Message: Dear Colleagues, please expose the potential candidates to the announcement below. Thank you in advance. Andrei Kolmakov Project Leader CNST NIST Energy Group NIST 100 Bureau Drive Gaithersburg, MD 20899-6204 Phone: (301) 975-4724 Fax: (301) 975-2303 E-mail: andrei.kolmakov-at-nist.gov
POSTDOCTORAL OPENING: SEM in liquids and gases
NIST CNST (Center for Nanoscale Science and Technology) has an active research program on development of in situ scanning electron microscopy and spectroscopy of objects and devices in liquids and dense gaseous environments using environmental cells equipped with electron transparent windows (including graphene). We have excellent experimental opportunities for this research line including clean room micro-fabrication facilities, array of dedicated SEM microscopes (VP JEOL 7800, UHV Zeiss column coupled with AES capabilities, Zeiss EVO coupled with micro-Raman spectrometer) and design/engineering support. Currently, a postdoctoral position for highly motivated and experienced experimentalist is available. The preferable set of skills includes but not limited to: liquid /atmospheric pressure SEM, electrochemistry, EBIC, XPS, AES, AFM, UHV surface science protocols, clean room experience,excellent writing and teamwork skills. The letter of interest, CV with a publication record and contact names of 3-4 references should be sent to Dr. Andrei Kolmakov, andrei.kolmakov-at-nist.gov Thank you for your interest
Andrei Kolmakov Project Leader CNST NIST Energy Group NIST 100 Bureau Drive Gaithersburg, MD 20899-6204 Phone: (301) 975-4724 Fax: (301) 975-2303
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Our 30+ year old single tilt holder, which is the spring-loaded "donut" clamp, failed after many decades of duty. One of the wires holder the donut clamp fractured on its own, and thus no longer clamps samples into the holder. We've had multiple issues across multiple different microscopes this month; Halloween is indeed a spooky time in a microscopy lab.
Anyway, does anyone have a donut type clamping single tilt holder compatible with the EM400, CM10, CM12, CM20, and/or CM30 goniometers for sale or for donation? We prefer the donut clamp over the circlip style holder, though we will consider anything on offer/for sale.
Thank you, Chris
==============================Original Headers============================== 4, 37 -- From microwink-at-gmail.com Wed Oct 26 18:43:38 2016 4, 37 -- Received: from mail-qk0-f172.google.com (mail-qk0-f172.google.com [209.85.220.172]) 4, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u9QNhZAe016313 4, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Oct 2016 18:43:35 -0500 4, 37 -- Received: by mail-qk0-f172.google.com with SMTP id x11so24011373qka.1 4, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Oct 2016 16:46:51 -0700 (PDT) 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=gmail.com; s=20120113; 4, 37 -- h=mime-version:from:date:message-id:subject:to; 4, 37 -- bh=TBnp4i56wkahMEugG9sRVhMS7+IDZTYcMraG7FnfyZc=; 4, 37 -- b=FYh5XYkFu6PAkXdPymTlnQNgg/8b+iDGLxHlx99bLqafMVecGGrcbtk1o4VdASvwPv 4, 37 -- GMRIc0nE9IPaL5kC+nPVorqRSlV92xpczGBmGzmVaSYn4hk0sFcMtcA9oI1XxaG0lagE 4, 37 -- Ws14Om61Wrw67E6Dd41hJ1oqHDe7/5NHk3WJXIqK66oe7F+En8CxZKR3WqBnIHK5XfVi 4, 37 -- KMimu/cytygpP1QVD6dOho3zbdu/FBtWhGywNj3KZKxMZ23TCTbKXcRlO1gPzch1JYK9 4, 37 -- 2N3OKHpJRUTr+pI0A2euXK8cLcSOhyly30s7Zue4ChBO9nqk3lVHFTLmwLw6xmUu0PxD 4, 37 -- AcVg== 4, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=1e100.net; s=20130820; 4, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 4, 37 -- bh=TBnp4i56wkahMEugG9sRVhMS7+IDZTYcMraG7FnfyZc=; 4, 37 -- b=cWTucR2eGPo1blyQpaEzNhw90ZTCOiuODP5rIBLwu0WIgL4xbhyX9fP+f+9/m17SVq 4, 37 -- LMEtKl9b5JiGoPBRZ5r+SXJPoNmo9ReqSHWAvhmASPwI0nI/4ki7tmrmcbB/QotzD7zV 4, 37 -- 1tzsRHbZZmxQhiVAxUm6Cm2OedFNDY1PsrCdpEJjKe7ZYQvkmzjqVFprgzhlCVrhOft0 4, 37 -- l4IoHBl88rMOIQQ0tha/WrvQwi9gM8BN9sezFdAjOOeAaBVegODIteyCzrP09gde6xRo 4, 37 -- zg8tHW4SdAL8zgyA8cSvRuQ87t1S5NU+V057gLbeDf9OaBAYu+s51Hqdzgj1Xo7uCWnJ 4, 37 -- zGmQ== 4, 37 -- X-Gm-Message-State: ABUngvc/CFWeuftpFh+4pKsEz/xSP+1EA5OZTMqAUxR+6Jfwtx8ybNTCW2jjG76CYDX/YKMKqrB0hGDQmIxaKg== 4, 37 -- X-Received: by 10.55.176.197 with SMTP id z188mr3667614qke.286.1477525610607; 4, 37 -- Wed, 26 Oct 2016 16:46:50 -0700 (PDT) 4, 37 -- MIME-Version: 1.0 4, 37 -- Received: by 10.200.45.253 with HTTP; Wed, 26 Oct 2016 16:46:49 -0700 (PDT) 4, 37 -- From: Christopher Winkler {microwink-at-gmail.com} 4, 37 -- Date: Wed, 26 Oct 2016 19:46:49 -0400 4, 37 -- Message-ID: {CAA8T2PO5UMjdRj-nfHW2Szy-2+4QwasBe1Bq2bT8keiuCEnvBw-at-mail.gmail.com} 4, 37 -- Subject: Seeking single tilt holder for EM400 series/CM10/CM12/CM20/CM30 TEM 4, 37 -- To: Microscopy-at-microscopy.com 4, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
not knowing the size of your vein or artery you previously processed (and how): Generally, I guess the treatment may have been to harsh with regard to perhaps the dissection procedure (how was the vessel razor-blade trimmed prior to fixation? , "full" fixation and perhaps conservative / mild dehydration etc.... I would not believe 'your' blood clot to disappear simply....Long ago I have done TEM and SEM studies / specimen preparations on (small and big) vessels (Arteria thoracica interna) which had to be evaluated for blood clot / thrombus formation after use of an ultrasonic aspirator/dissector device (cf: https://www.researchgate.net/publication/200468940_Effects_of_ultrasound_tre atment_of_the_arteria_thoracica_Amammaria_interna_ATI_during_preparation_for _coronary_artery_bypass_surgery_CABG_a_correlative_light_microscopic_scannin g_and_transmission_elec) Don't know if you are processing in a tissue processor... where you will have problems to find smallest pieces of tissue or substrate which are .... if you process manually in small snap-cap vials made from glass you perhaps will see such disrupted tissue material more easily.
Blood clots aren't easily/rapidly fixed by the usual procedere, so you might prolong your primary fixation (use buffered FA-GA fixative) at least 2x as usual or longer (use of a specimen rotator recommended!) also can use (low molecular weight) [e.g. 4%, 8%] tannic acid (or sometimes reported: gallic acid] in combination either with the primary fixative or also use Acrolein (if that is available for you [CAVE: Caution: Hazard] in combination with the primary fixative. Cf. also perhaps to https://www.ncbi.nlm.nih.gov/pubmed/7013112 (just as only ONE special literature reference)
Best regards and wishes, Happy Halloween.... Wolfgang
Wolfgang MUSS PhD Retired from EM-LAb Emeritus member of MSA SALZBURG, AUSTRIA
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Email: andy.stewart-at-ul.ie Name: Andy Stewart
Organization: University of Limerick
Title-Subject: [Filtered] Post Doctoral Researcher – Electron Microscopist (Deadline 2nd November)
Message: TITLE OF POST: Post Doctoral Researcher Electron Microscopist LOCATION: University of Limerick REPORTS TO: Director of Bernal Institute/Nominee of the Director SALARY: €37,750 - €46,255 p.a. (maximum starting salary €42,394 p.a.)
QUALIFICATIONS: A doctoral degree (level 10 NFQ) in materials science/electron microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent experience in a research environment with at least 2 years’ experience in the area of electron microscopy applied to materials characterisation.
OVERALL PURPOSE OF THE JOB: To manage and develop the electron microscopy suite at the University of Limerick, to support research activity in the Institute in the area of materials characterisation and to carry out original and significant research in this area.
Essential Criteria Applicants must have: •A doctoral degree (level 10 NFQ) in materials science/electron microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent experience in a research environment with at least 2 years’ experience in the area of electron microscopy applied to materials characterisation.•Hands-on experience in electron microscopy and be able to train new users in this technique.•Demonstrable knowledge of materials science, particularly related to the areas of materials for energy and environment, nanomaterials, pharmaceutical materials, biomedical engineering and biomaterials, and composite materials. •Evidence of ability to work individually and as part of a team. •Good oral and written communication skills. •Potential to write research reports and develop research proposals in the field of materials, in collaboration with other researchers. •A strong track record of recent publications in peer reviewed journals, with at least three journal papers indexed on Web of Science.
Desirable Criteria •Experience in Raman spectroscopy. •Experience of working with industry partners to solve industrially relevant materials-science orientated problems.
Instrumentation available -- Double aberration corrected, monochromatic FEI Titan Cubed Thermis 30 TEM with analytical facilities (SuperEDX and QuantumEELS), which will provide a unique capability in Ireland for ultra-high resolution imaging and spectroscopic analysis of materials.
-- JEOL JEM-2100F with a field emission source, including bright-/dark field imaging, a STEM attachment, which simultaneously obtains bright field (BF) and high angle annular dark field (HAADF) images, a SEI/BSE detector, an energy dispersive X-ray analyser and computer controlled 3D reconstruction high angle tomography. -- JEOL JEM-2011 with a LaB6 source, STEM including bright-/dark field imaging, an energy dispersive X-ray analyser.
--Hitachi SU-70 scanning electron microscope with a thermal field emission source, back scattered electron (BSE) detector, energy dispersive X-ray spectroscopy (EDS), wavelength dispersive X-ray spectroscopy (WDS) and an electron back scattered diffraction detector (EBSD); a coupled SEM-Raman system for simultaneous chemical characterisation coupled with high resolution imaging
Further information for applicants and application material is available online from: http://www.ul.ie/hrvacancies/ The closing date for receipt of applications via online completion is, 2nd of November 2016, by 12 noon, Irish Standard Time.
Please email erecruitment-at-ul.ie if you experience any difficulties; queries can also be directed to Noel.ODowd-at-ul.ie or Ursel.Bangert-at-ul.ie
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This semester I have the pleasure of teaching a hands-on SEM course for undergraduates. In my last lecture in a couple of weeks, I'd like to talk a bit about the types of careers in the SEM field. I have a good idea of the types of jobs in academia and some idea of the more industry-type jobs, but I think it would be really great if people could chime in with their specific jobs and what types of samples you look at. Real world examples are always easier to grasp, I think.
Also, if you know of any certified training courses (besides Lehigh), please share that information.
Any information is greatly appreciated!
Thank you, -Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
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Email: alkoh-at-stanford.edu Name: Ai Leen Koh
Organization: Stanford University
Title-Subject: [Filtered] Stanford Imaging Workshop: Advanced Transmission Electron Microscopy for In-situ and Environmental Studies
Message: Thursday, Dec 8, 2016 8:30 am - 5:15 pm Stanford University Stanford Nano Shared Facilities Stanford, CA 94305-4088 Stanford Nano Shared Facilities (SNSF) invites you to attend this unique workshop demonstrating the applications of high-speed imaging, electron energy loss spectroscopy, in-situ, and environmental (scanning) transmission electron microscopy ((S)TEM). This comprehensive, full-day workshop will feature lectures by leading experts, open Q&A sessions, and live microscope demonstrations on gas-solid reactions at the state-of-the-art SNSF research complex. The microscope sessions will be performed on an aberration (image) corrected, monochromated FEI Titan environmental (S)TEM equipped with a Gatan OneView in-situ camera, Gatan GIF Quantum ERS imaging filter, plus a Wildfire heating holder and Climate 3+ flow gas system by DENSsolutions. Registration is free. All confirmed participants will receive a complimentary breakfast, lunch, and refreshments. Program and registration details online at: http://www.gatan.com/company/events/stanford-imaging-workshop-advanced-transmission-electron-microscopy-situ-and
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Hello, We have an opening for a Microsopy Assistant to work at the Johns Hopkins SOM Microsocpe Facility (est.1989). We are seeking a talented individual to preform both electron microscopy and some confocal/fluorescence procedures. We have several electron and confocal microscopes. This is an excellent opportunity for new graduates to learn new skills and fine tune your techniques in microscopy. Please apply through the Johns Hopkins SOM HR dept:
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] European EELS & EFTEM School at Graz University
Message: The joint FELMI/European EELS & EFTEM School is an intense 4-day hands-on laboratory workshop that will take you step-by-step through the acquisition and analysis of Energy Filtered TEM (EFTEM) and STEM-EELS data. The workshop will utilize the state of the art facilities at FELMI-ZFE including a monochromated probe-corrected Titan (S)TEM with a DualEELS GIF Quantum EELS system.
Our qualified staff will familiarize you with the latest EELS & EFTEM equipment and will teach you fundamental principles and methods which are crucial to take top quality EELS spectra, STEM-EELS spectrum images and energy-filtered images or elemental maps. While not a focus of the workshop, optimization of the source monochromator for high-resolution EELS and the Cs probe corrector for STEM-EELS is included in the program.
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Check out the exciting TEM advance in the current issue of Cell Chemical Biology. "Detailed view of biological structures is created by overlaying conventional electron micrographs with pseudocolor lanthanide elemental maps derived from distinctive electron energy-loss spectra of each lanthanide deposit via energy-filtered transmission electron microscopy." http://www.cell.com/cell-chemical-biology/fulltext/S2451-9456(16)30357-9
Thomas E. Phillips, Ph.D Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Dear Colleagues, Could you please pass the following information to potential candidates in your group and/or beyond? Thank you in advance Andrei Kolmakov
NIST Center for Nanoscale Science and Technology is running an exciting program on electron microscopy (SEM) and spectroscopy (AES, XPS) of objects, interfaces, and devices in liquids and dense gaseous environments using graphene-based and traditional SiN membranes coupled with closed or fluidic cells. In addition to excellent microfabrication facilities, we have an array of dedicated new experimental systems which include: variable pressure SEM, UHV Scanning Auger System, XPS system, micro-Raman /optical spectrometer coupled with SEM as well as setups for in situ electrical measurements. Currently, a postdoctoral position for highly motivated and experienced experimentalist is available. The preferable set of skills includes but not limited: hands-on experience with SEM, XPS, AES, UHV surface science protocols, clean room experience, electrochemistry, excellent writing and teamwork skills and etc. The letter of interest, CV with publication record and contact information of 3-4 references should be e-mailed to
Dr. Andrei Kolmakov
Thank you for your interest!
Andrei Kolmakov, PhD
Project Leader Center for Nanoscale Science and Technology National Institute of Standards and Technology
can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Please suggest (a) type of specimen grid / support film and (b) preparation methods for minimizing specimen drift. Conditions: - 120 to 200 kV; - medium spot size; - 180kx to 1000kx TEM mag. - non-organic specimens I am reasonably sure drift comes from specimen support film, not from TEM column/stage or specimen holder. Drift will slow down to acceptable level after sample irradiated by concentrated beam for 10+ seconds or by wide-spread beam for some 10 minutes - not desirable either way.
-- Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
==============================Original Headers============================== 4, 30 -- From vitalylazar-at-att.net Sun Nov 6 16:04:33 2016 4, 30 -- Received: from nm1-vm2.access.bullet.mail.bf1.yahoo.com (nm1-vm2.access.bullet.mail.bf1.yahoo.com [216.109.114.73]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uA6M4Xmp025548 4, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Nov 2016 16:04:33 -0600 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=att.net; s=s1024; t=1478469922; bh=uFia4B/8WYWgUCm9DGqkjEV6zKFBMDW1HmLprdnl4yQ=; h=Date:From:To:Subject:From:Subject; b=lU46VHCiS+7Ar1gSpY/5actVgal1kZHD+C2fNOiD8WaOO0vsb9Jmi4s4UULaMVGOeQ7wytPQ+0Uo40v8pG65W5WIloD5Az3J5KpPTYmUYjJkZkUlT+XY4UHsXzdskSu5wx0qZRDVBojju2g6ggN61q2otWfHWTMmZNLCdgQ314M= 4, 30 -- Received: from [66.196.81.163] by nm1.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Nov 2016 22:05:22 -0000 4, 30 -- Received: from [98.138.226.243] by tm9.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Nov 2016 22:05:22 -0000 4, 30 -- Received: from [127.0.0.1] by smtp114.sbc.mail.ne1.yahoo.com with NNFMP; 06 Nov 2016 22:05:22 -0000 4, 30 -- X-Yahoo-Newman-Id: 457682.84335.bm-at-smtp114.sbc.mail.ne1.yahoo.com 4, 30 -- X-Yahoo-Newman-Property: ymail-3 4, 30 -- X-YMail-OSG: DB9OB_wVM1n6H5hw4PypCkw8P9rlNDtO2sHmqW7MIJ5vcd. 4, 30 -- 2eNvlgcswzZu5RCKYJ.x7lpX2gVJ18ErVz31jex2OjSwPNwZiAQHrRZh.82t 4, 30 -- 02sqOY3IrUWeXkR89u7PD66jax22lCFgSwIsz4vNmbDYnOqYBF8nanYSXNh1 4, 30 -- 2YTSUUDaACbOTugASdQeavqXuglzdnwBwdwlpmdCq_F51ajYwc4eDIVn_30c 4, 30 -- hcIeHjJ9vEOl6G0lkTzuAaY6Zf2V_QKL24MAp.shCGN53XFiztysVk0yPAVS 4, 30 -- WbIVNIJuqXUUKuTieeuwx65KjDLcEr89CtgM0geZd5AQr3Jkm4VEOSidAsVO 4, 30 -- CTdgy0r7x_ROuGWmJAQYbnaHky38lRpwKtxAZHtW9DEoPtE7P49jozDij9Nx 4, 30 -- t77eHwOTYr_8xgX3HrYetSpXwDeeSSay3OZEq2e2FJcqY.KBYAAj0lDVVhub 4, 30 -- pk1BZtteUbNEgMbjg2Q5o4BU07Z2bxxNTaM8jlw5Um7Mok5wFtdAL1t4FGFm 4, 30 -- qScjcqduu5jfEotEUH5WxHb2Ww4X_CLfn6oha3EkfYCe.7f3GA13leqo- 4, 30 -- X-Yahoo-SMTP: gj.qIISswBA.S8Qm9l.Bw9ouZ8hEcT1EKbhSAYjFDs8- 4, 30 -- Message-ID: {581FA923.4030708-at-att.net} 4, 30 -- Date: Sun, 06 Nov 2016 17:05:23 -0500 4, 30 -- From: Vitaly Feingold {vitalylazar-at-att.net} 4, 30 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 4, 30 -- MIME-Version: 1.0 4, 30 -- To: microscopy Listserver {Microscopy-at-microscopy.com} 4, 30 -- Subject: specimen drift 4, 30 -- Content-Type: text/plain; charset=utf-8; format=flowed 4, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Vitaly, Have you tried carbon coating your support film (carbon, parlodion or formvar)? If I get drift on the TEM I will adjust the spot size and/or flament current to minimize at high magnifications. Good Luck, Michael Delannoy
-----Original Message----- X-from: vitalylazar-at-att.net [mailto:vitalylazar-at-att.net] Sent: Sunday, November 06, 2016 5:14 PM To: delannoy-at-jhmi.edu
Dear Listers,
Please suggest (a) type of specimen grid / support film and (b) preparation methods for minimizing specimen drift. Conditions: - 120 to 200 kV; - medium spot size; - 180kx to 1000kx TEM mag. - non-organic specimens I am reasonably sure drift comes from specimen support film, not from TEM column/stage or specimen holder. Drift will slow down to acceptable level after sample irradiated by concentrated beam for 10+ seconds or by wide-spread beam for some 10 minutes - not desirable either way.
-- Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
==============================Original Headers============================== 4, 30 -- From vitalylazar-at-att.net Sun Nov 6 16:04:33 2016 4, 30 -- Received: from nm1-vm2.access.bullet.mail.bf1.yahoo.com (nm1-vm2.access.bullet.mail.bf1.yahoo.com [216.109.114.73]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uA6M4Xmp025548 4, 30 -- for {Microscopy-at-microscopy.com} ; Sun, 6 Nov 2016 16:04:33 -0600 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=att.net; s=s1024; t=1478469922; bh=uFia4B/8WYWgUCm9DGqkjEV6zKFBMDW1HmLprdnl4yQ=; h=Date:From:To:Subject:From:Subject; b=lU46VHCiS+7Ar1gSpY/5actVgal1kZHD+C2fNOiD8WaOO0vsb9Jmi4s4UULaMVGOeQ7wytPQ+0 Uo40v8pG65W5WIloD5Az3J5KpPTYmUYjJkZkUlT+XY4UHsXzdskSu5wx0qZRDVBojju2g6ggN61q 2otWfHWTMmZNLCdgQ314M= 4, 30 -- Received: from [66.196.81.163] by nm1.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Nov 2016 22:05:22 -0000 4, 30 -- Received: from [98.138.226.243] by tm9.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Nov 2016 22:05:22 -0000 4, 30 -- Received: from [127.0.0.1] by smtp114.sbc.mail.ne1.yahoo.com with NNFMP; 06 Nov 2016 22:05:22 -0000 4, 30 -- X-Yahoo-Newman-Id: 457682.84335.bm-at-smtp114.sbc.mail.ne1.yahoo.com 4, 30 -- X-Yahoo-Newman-Property: ymail-3 4, 30 -- X-YMail-OSG: DB9OB_wVM1n6H5hw4PypCkw8P9rlNDtO2sHmqW7MIJ5vcd. 4, 30 -- 2eNvlgcswzZu5RCKYJ.x7lpX2gVJ18ErVz31jex2OjSwPNwZiAQHrRZh.82t 4, 30 -- 02sqOY3IrUWeXkR89u7PD66jax22lCFgSwIsz4vNmbDYnOqYBF8nanYSXNh1 4, 30 -- 2YTSUUDaACbOTugASdQeavqXuglzdnwBwdwlpmdCq_F51ajYwc4eDIVn_30c 4, 30 -- hcIeHjJ9vEOl6G0lkTzuAaY6Zf2V_QKL24MAp.shCGN53XFiztysVk0yPAVS 4, 30 -- WbIVNIJuqXUUKuTieeuwx65KjDLcEr89CtgM0geZd5AQr3Jkm4VEOSidAsVO 4, 30 -- CTdgy0r7x_ROuGWmJAQYbnaHky38lRpwKtxAZHtW9DEoPtE7P49jozDij9Nx 4, 30 -- t77eHwOTYr_8xgX3HrYetSpXwDeeSSay3OZEq2e2FJcqY.KBYAAj0lDVVhub 4, 30 -- pk1BZtteUbNEgMbjg2Q5o4BU07Z2bxxNTaM8jlw5Um7Mok5wFtdAL1t4FGFm 4, 30 -- qScjcqduu5jfEotEUH5WxHb2Ww4X_CLfn6oha3EkfYCe.7f3GA13leqo- 4, 30 -- X-Yahoo-SMTP: gj.qIISswBA.S8Qm9l.Bw9ouZ8hEcT1EKbhSAYjFDs8- 4, 30 -- Message-ID: {581FA923.4030708-at-att.net} 4, 30 -- Date: Sun, 06 Nov 2016 17:05:23 -0500 4, 30 -- From: Vitaly Feingold {vitalylazar-at-att.net} 4, 30 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 4, 30 -- MIME-Version: 1.0 4, 30 -- To: microscopy Listserver {Microscopy-at-microscopy.com} 4, 30 -- Subject: specimen drift 4, 30 -- Content-Type: text/plain; charset=utf-8; format=flowed 4, 30 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 11, 24 -- From prvs=11236521f=mdelann1-at-jhmi.edu Mon Nov 7 08:21:51 2016 11, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.199.159]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uA7ELp1d020940 11, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Nov 2016 08:21:51 -0600 11, 24 -- X-IronPort-AV: E=Sophos;i="5.31,606,1473134400"; 11, 24 -- d="scan'208";a="58824171" 11, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 11, 24 -- by IronEB5.johnshopkins.edu with ESMTP/TLS/DHE-RSA-AES256-SHA; 07 Nov 2016 09:22:41 -0500 11, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 11, 24 -- To: {vitalylazar-at-att.net} , {Microscopy-at-microscopy.com} , 11, 24 -- {microscopy-at-msa.microscopy.com} , 11, 24 -- {microscopylistserver-noreply-at-microscopy.com} 11, 24 -- References: {201611062213.uA6MDpTZ032265-at-ns.microscopy.com} 11, 24 -- In-Reply-To: {201611062213.uA6MDpTZ032265-at-ns.microscopy.com} 11, 24 -- Subject: RE: [Microscopy] specimen drift 11, 24 -- Date: Mon, 7 Nov 2016 09:22:46 -0500 11, 24 -- Message-ID: {005501d23902$69055280$3b0ff780$-at-jhmi.edu} 11, 24 -- MIME-Version: 1.0 11, 24 -- Content-Type: text/plain; 11, 24 -- charset="us-ascii" 11, 24 -- Content-Transfer-Encoding: 7bit 11, 24 -- X-Mailer: Microsoft Outlook 14.0 11, 24 -- Thread-Index: AQGCn0B3KT5rLwJwzLH/1qZx2fsIG6FsxXAQ 11, 24 -- Content-Language: en-us ==============================End of - Headers==============================
________________________________________ X-from: stefan.diller-at-t-online.de {stefan.diller-at-t-online.de} Sent: Saturday, November 5, 2016 7:30 AM To: Sun, Xuanhao
Dear All,
can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Specimen drift in the TEM generally has 2 causes: thermal or charging.
Thermal drift can arise when the room temperature is different from the internal temperature of the pole-piece area. When you insert the sample rod, it can take quite a while to thermally equilibrate. The 2nd cause of thermal drift can be the local warming of the sample by the e-beam. There are many aspects of the sample that can affect this: thermal conductivity of the sample (support film, etc), electron flux, mean free path of the electrons in the sample, etc.
Sample charging can also lead to drift but it may also lead to more of an instability as the sample charges up and discharges.
X-from your description, it sounds like local sample heating is the most likely source of your drift especially if you don't notice drift on other samples. Using a carbon support film rather than a polymer support film may improve things.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
thanks for getting the manual. No more help needed.
This group is one-of-a-kind ;-)
Best,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I did it today and will send after voting tomorrow.
Fred
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu ________________________________________ X-from: xuanhao.sun-at-uconn.edu [xuanhao.sun-at-uconn.edu] Sent: Monday, November 7, 2016 9:53 AM To: Monson, Frederick
Hi,
I can have one of my colleagues scan and send you the pdf tomorrow if you havent received that from other source by that time. Let me know and thanks!
Xuanhao Sun, Ph.D.
Director of Operations Bioscience Electron Microscopy Laboratory University of Connecticut BPB G06, Unit 3242 Storrs, CT 06269-3242
________________________________________ X-from: stefan.diller-at-t-online.de {stefan.diller-at-t-online.de} Sent: Saturday, November 5, 2016 7:30 AM To: Sun, Xuanhao
Dear All,
can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Is it safer to have the sputter coater in a fume hood when sputter coating with chromium? (As opposed to just sitting on the lab bench) This comes up because of a request to sputter coat a large number of test strips with chromium. I couldn't find any safety warnings in this regard, and I don't think there is much risk. Gloves will be worn when handling the strips. Any advice would be much appreciated.
J. Scott LeMaster Senior Research Chemist II Analytical PPG
________________________________
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Organization: Max Planck Florida Institute for Neuroscience
Title-Subject: [Filtered] MPFI Neuroimaging Course - EM module
Message: Max Planck Florida Institute for Neuroscience will host an intensive and comprehensive laboratory-oriented course focusing on applying imaging techniques to neuroscience research. The objective of this imaging course is to gain exposure to modern imaging tools from principle optics to applications in modern neuroscience.
Please see details; https://www.maxplanckflorida.org/education/courses/LM-course/NIT-2017
Electron Microscopy Facility will host a "correlative LM-EM" module in the course. EM module attendees have full access to all talks offered in the course and will focus on CLEM projects. Attendees are encouraged to bring own their samples upon discussion and approval. Registration Deadline; November 28, 2016
Contact: Jessica Herbst for general questions (jessica.herbst-at-mpfi.org) Contact: Naomi Kamasawa (naomi.kamasawa-at-mpfi.org) for EM module questions
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Title-Subject: [Filtered] infiltrating large samples
Message: Hello listers. We have very large brain samples that are loaded with heavy metals, 1.5 mm in thickness. Good infiltration is imperative. I am using Hard Plus resin because of its consistency I cannot work with Spurr as I am allergic to it. The intermediate fluid is acetonitrile.
Infiltrating in resin without accelerator seems best, my question is, which step of the infiltration process should be the most effective - 2:1, 1;1. 1:2, 100%. Right now each step is 24-48 hours except fresh 100% w accelerator is 4-6 hr. Overall protocol is 9 days with 96 hours of block hardening.
Right now our blocks have small ripped areas in the semi thins. Overall infiltration is OK, but I need to get rid of these chipped/ripped areas.
I am thinking longer in 100% resin with accelerator before embedding. Any thoughts, ideas?
thanks in advance. JoAnn
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As far as I know chromium in its metallic form is not considered as toxic otherwise everyone should handle stainless steel with latex gloves:-). But then your sputter coater has a vacuum pump right? So you should care about the exhaust in this case. Just the 2 cents of a cell biologist who was once an electron microscopist.
regards Stephane
-------------------------------------------- On Wed, 11/9/16, lemaster-at-ppg.com {lemaster-at-ppg.com} wrote:
Subject: [Microscopy] SEM - Safety of chromium sputter coater To: nizets2-at-yahoo.com Date: Wednesday, November 9, 2016, 6:48 PM
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Is it safer to have the sputter coater in a fume hood when sputter coating with chromium? (As opposed to just sitting on the lab bench) This comes up because of a request to sputter coat a large number of test strips with chromium. I couldn't find any safety warnings in this regard, and I don't think there is much risk. Gloves will be worn when handling the strips. Any advice would be much appreciated.
J. Scott LeMaster Senior Research Chemist II Analytical PPG
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Help Us Create Amazing Microscopy Programs for Museum Audiences! The Smithsonian National Museum of Natural History is looking for a Microscopy Educator. This is a two year term limited full time staff position at the National Museum of Natural History in Washington DC. We are looking for the right person to produce a set of experiential activities, programs and training workshops about microscope technology and natural history science for a diversity of non-specialist audiences, specifically youth ages 14-18 and their families and teachers. We are looking for a unique individual who has a mix of skills and experiences pertaining to microscopy, informal science learning environments, and teen audiences to be a part of the Education and Outreach team. If you or someone you know has the following skills and experiences, please be in touch. We are looking for individuals with the following:
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==============================Original Headers============================== 13, 26 -- From WHITTAKS-at-si.edu Mon Nov 14 12:41:46 2016 13, 26 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAEIfkph004499 13, 26 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2016 12:41:46 -0600 13, 26 -- Received: from SI-MSECHT02.US.SINET.SI.EDU (si-msecht02.us.sinet.si.edu [160.111.49.154]) 13, 26 -- by si-mailout04.si.edu (Postfix) with ESMTP id 6BD45E6294 13, 26 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2016 13:43:04 -0500 (EST) 13, 26 -- Received: from SI-MSEDAG04.US.SINET.SI.EDU ([169.254.4.106]) by 13, 26 -- SI-MSECHT02.US.SINET.SI.EDU ([160.111.49.154]) with mapi id 14.03.0319.002; 13, 26 -- Mon, 14 Nov 2016 13:43:03 -0500 13, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 13, 26 -- To: "Whittaker, Scott" {WHITTAKS-at-si.edu} 13, 26 -- Subject: LM/SEM- Microscopy Educator Position- Wash. DC 13, 26 -- Thread-Topic: LM/SEM- Microscopy Educator Position- Wash. DC 13, 26 -- Thread-Index: AdI+oCcFvkIekn2cRfK9XhFI2Kfjsw== 13, 26 -- Date: Mon, 14 Nov 2016 18:43:02 +0000 13, 26 -- Message-ID: {5D4F637E5C599649A11693E3B6EAC6293FC0C0EF-at-si-msedag04.US.SINET.SI.EDU} 13, 26 -- Accept-Language: en-US 13, 26 -- Content-Language: en-US 13, 26 -- X-MS-Has-Attach: 13, 26 -- X-MS-TNEF-Correlator: 13, 26 -- x-originating-ip: [160.111.49.170] 13, 26 -- Content-Type: text/plain; charset="iso-2022-jp" 13, 26 -- MIME-Version: 1.0 13, 26 -- Content-Transfer-Encoding: 8bit 13, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uAEIfkph004499 ==============================End of - Headers==============================
Dear Colleagues, on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge is faulty and needs to be replaced (or does anybody know how to repair? we would need the W wire - can it be replaced? easy? is it W?) . It is an Edwards PRE10K (D02428000) - and we asked Edwards and they told us that this Pirani is not available - any more. Hmm. Does anybody have one for sale which is not used any more? Does anybody know another type which would fit into the Scancoat Six? Any help is much appreciated. kind regards, Reinhard Rachel
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: - Microscopy Conference 2017 (D-A-CH) Lausanne, CH 20-25 August 2017 http://www.mc2017.ch/ - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018 http://imc19.com/ - next Microbiol. conferences: VAAM/DGHM - Annual Conf March 5-8, 2017, Wuerzburg
==============================Original Headers============================== 6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15 04:39:13 2016 6, 23 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAFAdCBH003403 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 04:39:13 -0600 6, 23 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 6, 23 -- by localhost (Postfix) with SMTP id 500835AE01 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 +0100 (CET) 6, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 6, 23 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 377F45AE3B 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 +0100 (CET) 6, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 6, 23 -- with Novell_GroupWise; Tue, 15 Nov 2016 11:40:33 +0100 6, 23 -- Message-Id: {582AF42E020000540006412D-at-gwsmtp1.uni-regensburg.de} 6, 23 -- X-Mailer: Novell GroupWise Internet Agent 14.2.1 6, 23 -- Date: Tue, 15 Nov 2016 11:40:30 +0100 6, 23 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 6, 23 -- To: "microscopy server" {Microscopy-at-microscopy.com} 6, 23 -- Subject: Pirani 6, 23 -- Mime-Version: 1.0 6, 23 -- Content-Type: text/plain; charset=US-ASCII 6, 23 -- Content-Disposition: inline 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uAFAdCBH003403 ==============================End of - Headers==============================
Hello! To my knowledge, after Edwards dropped this kind of equipment from their portfolio, the Indian company HHV continues to market these instruments, and to sell spare parts for it. I once heard rumors that HHV already before would have been the place where this Edwards equipment was built. If this would be true, then contacting HHV would be the way to contact the manufacturer directly ;-) . There webpage states: "We hold an exclusive licence from Edwards to manufacture and supply the well-known ScanCoat Six, Auto306, Auto500 and forensic deposition systems under the HHV brand and have added our own range of systems to our product line-up." (http://www.hhvltd.com/). Good Luck to find your spare parts there! Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mller
---------------------------------------------------------------- Marco Mller Platform Manager - Electron Microscopy
CIC biomaGUNE Parque Tecnolgico de San Sebastin Paseo Miramn, 182. Ed. Empresarial C 20009 San Sebastin (Guipzcoa) SPAIN
Tel: +34 943 00 53 25 mmoller-at-cicbiomagune.es
} -----Original Message----- } From: Reinhard.Rachel-at-biologie.uni-regensburg.de } [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de] } Sent: Tuesday, November 15, 2016 11:54 AM } To: Marco Moller } Subject: [Microscopy] Pirani } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dear Colleagues, } on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge is } faulty and needs to be replaced (or does anybody know how to repair? we } would need the W wire - can it be replaced? easy? is it W?) . } It is an Edwards PRE10K (D02428000) - and we asked Edwards and they } told us that this Pirani is not available - any more. Hmm. } Does anybody have one for sale which is not used any more? } Does anybody know another type which would fit into the Scancoat Six? } Any help is much appreciated. } kind regards, } Reinhard Rachel } } -- } Prof. Dr. Reinhard Rachel } University of Regensburg } Centre for EM / Anatomy } Faculty of Biology & Preclin. Med. } Universitaetsstrasse 31 } D-93053 Regensburg - Germany } tel +49 941 943 -2837, -1720 } mail reinhard.rachel-at-biologie.uni-regensburg.de } office: VKL 3.1.29 } } Next microscopy conferences: } - Microscopy Conference 2017 (D-A-CH) Lausanne, CH } 20-25 August 2017 } http://www.mc2017.ch/ } - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018 } http://imc19.com/ } - next Microbiol. conferences: } VAAM/DGHM - Annual Conf } March 5-8, 2017, Wuerzburg } } } } } } ==============================Original } Headers============================== } 6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15 } 04:39:13 2016 } 6, 23 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni- } regensburg.de [194.94.155.51]) } 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id uAFAdCBH003403 } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 04:39:13 } -0600 } 6, 23 -- Received: from rrzmta1.uni-regensburg.de (localhost } [127.0.0.1]) } 6, 23 -- by localhost (Postfix) with SMTP id 500835AE01 } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 } +0100 (CET) } 6, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni- } regensburg.de [132.199.5.51]) } 6, 23 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id } 377F45AE3B } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 } +0100 (CET) } 6, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni- } regensburg.de } 6, 23 -- with Novell_GroupWise; Tue, 15 Nov 2016 11:40:33 +0100 } 6, 23 -- Message-Id: {582AF42E020000540006412D-at-gwsmtp1.uni- } regensburg.de} } 6, 23 -- X-Mailer: Novell GroupWise Internet Agent 14.2.1 } 6, 23 -- Date: Tue, 15 Nov 2016 11:40:30 +0100 } 6, 23 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni- } regensburg.de} } 6, 23 -- To: "microscopy server" {Microscopy-at-microscopy.com} } 6, 23 -- Subject: Pirani } 6, 23 -- Mime-Version: 1.0 } 6, 23 -- Content-Type: text/plain; charset=US-ASCII } 6, 23 -- Content-Disposition: inline } 6, 23 -- Content-Transfer-Encoding: 8bit } 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id uAFAdCBH003403 } ==============================End of - } Headers==============================
Hello! To my knowledge, after Edwards dropped this kind of equipment from their portfolio, the Indian company HHV continues to market these instruments, and to sell spare parts for it. I once heard rumors that HHV already before would have been the place where this Edwards equipment was built. If this would be true, then contacting HHV would be the way to contact the manufacturer directly ;-) . There webpage states: "We hold an exclusive licence from Edwards to manufacture and supply the well-known ScanCoat Six, Auto306, Auto500 and forensic deposition systems under the HHV brand and have added our own range of systems to our product line-up." (http://www.hhvltd.com/). Good Luck to find your spare parts there! Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mller.
---------------------------------------------------------------- Marco Mller Platform Manager - Electron Microscopy CIC biomaGUNE Parque Tecnolgico de San Sebastin Paseo Miramn, 182. Ed. Empresarial C 20009 San Sebastin (Guipzcoa) SPAIN Tel: +34 943 00 53 25 mmoller-at-cicbiomagune.es
} } Dear Colleagues, } on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge } is faulty and needs to be replaced (or does anybody know how to } repair? we would need the W wire - can it be replaced? easy? is it W?) . } It is an Edwards PRE10K (D02428000) - and we asked Edwards and they } told us that this Pirani is not available - any more. Hmm. } Does anybody have one for sale which is not used any more? } Does anybody know another type which would fit into the Scancoat Six? } Any help is much appreciated. } kind regards, } Reinhard Rachel }
==============================Original Headers============================== 6, 37 -- From mmoller-at-cicbiomagune.es Tue Nov 15 09:11:57 2016 6, 37 -- Received: from webmail.cicbiomagune.es (webmail.cicbiomagune.es [150.241.252.34]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAFFBuNF004481 6, 37 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Nov 2016 09:11:57 -0600 6, 37 -- X-AuditID: 0a0d0165-1dfff70000000ce6-c5-582b260be24e 6, 37 -- Received: from CICMGEXCAS.cicbiomagune.int (cicmgexcas.cicbiomagune.int [10.10.1.3]) 6, 37 -- by webmail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id AA.5C.03302.B062B285; Tue, 15 Nov 2016 16:13:16 +0100 (CET) 6, 37 -- Received: from CICMGEXMBX.cicbiomagune.int ([::1]) by 6, 37 -- CICMGEXCAS.cicbiomagune.int ([::1]) with mapi id 14.03.0195.001; Tue, 15 Nov 6, 37 -- 2016 16:13:15 +0100 6, 37 -- From: Marco Moller {mmoller-at-cicbiomagune.es} 6, 37 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 6, 37 -- Subject: RE: Pirani 6, 37 -- Thread-Topic: Pirani 6, 37 -- Thread-Index: AQHSPy7LerwOdSAn50yi5LDooZ51OqDaI8jggAACwmA= 6, 37 -- Date: Tue, 15 Nov 2016 15:13:15 +0000 6, 37 -- Message-ID: {216351DE18DE144BBBFC1996E5FAEBB2048A00-at-CICMGEXMBX.cicbiomagune.int} 6, 37 -- Accept-Language: de-DE, es-ES, en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- x-originating-ip: [10.10.2.151] 6, 37 -- Content-Type: text/plain; charset="iso-8859-1" 6, 37 -- MIME-Version: 1.0 6, 37 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFrrMLMWRmVeSWpSXmKPExsXCxcXIrMujph1h0L5XweLP4QZ2B0aPj92v 6, 37 -- mQIYo7hsUlJzMstSi/TtErgyPl57y1JwkLdi2b1GlgbGX1xdjJwcEgImEm1rHjJ2MXJxCAlc 6, 37 -- Z5Q4uL4FypnFKPG/4w07SBWbgI7Eo9PXWUBsEQFriUsvt7GB2MICwhKb/i6FiotI3FyzGMq2 6, 37 -- knh7YA8TiM0ioCrx88cusDivQIDEnxNPgOIcHIwCKhIbXumChJkFxCVuPZnPBHGQgMSSPeeZ 6, 37 -- IWxRiZeP/7GClEsIyEvcmuYIUa4ncWPqFDYIW1ti2cLXzBDTBSVOznzCMoFRaBaSqbOQtMxC 6, 37 -- 0jILScsCRpZVjNJJRZnpGSW5iZk5esmZyUmZ+bmJ6aV5qXrA4N7ECApqXsbUHYz7J+oeYhTg 6, 37 -- YFTi4RU8oRkhxJpYVlyZe4hRgoNZSYRXUUI7Qog3JbGyKrUoP76oNCe1+BCjNAeLkjivle/v 6, 37 -- cCGB9MSS1OzU1ILUIpgsEwenVAPj/JOFe6/ZCK1SqVhg9nLruarE+0s/2n26sEin6uFUlfaJ 6, 37 -- AVqui7Kcf3z7dPLiSwUVU27JP/dlTZ3r7ad72xooip//zOmzwTzr05rnfMdL2rh69p8tvKy6 6, 37 -- 9tJf90tubYzXg/4E6QRVTF//JfvuFbcD8pZymW+eb0jN3v735I2UJrFlP86onrL+psRSnJFo 6, 37 -- qMVcVJwIAGhmSWFmAgAA 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uAFFBuNF004481 ==============================End of - Headers==============================
Reinhard, If you can dismantle the gauge and get to the filament you can try sonicating in mild HCL solution. I do this routinely with my penning gauge on my FESEM when it gets coated with carbon. Sincerely,
Michael Delannoy
-----Original Message----- X-from: mmoller-at-cicbiomagune.es [mailto:mmoller-at-cicbiomagune.es] Sent: Tuesday, November 15, 2016 10:16 AM To: delannoy-at-jhmi.edu
Hello! To my knowledge, after Edwards dropped this kind of equipment from their portfolio, the Indian company HHV continues to market these instruments, and to sell spare parts for it. I once heard rumors that HHV already before would have been the place where this Edwards equipment was built. If this would be true, then contacting HHV would be the way to contact the manufacturer directly ;-) . There webpage states: "We hold an exclusive licence from Edwards to manufacture and supply the well-known ScanCoat Six, Auto306, Auto500 and forensic deposition systems under the HHV brand and have added our own range of systems to our product line-up." (http://www.hhvltd.com/). Good Luck to find your spare parts there! Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mvller
---------------------------------------------------------------- Marco Mvller Platform Manager - Electron Microscopy
CIC biomaGUNE Parque Tecnolsgico de San Sebastian Paseo Miramsn, 182. Ed. Empresarial C 20009 San Sebastian (Guipzzcoa) SPAIN
Tel: +34 943 00 53 25 mmoller-at-cicbiomagune.es
} -----Original Message----- } From: Reinhard.Rachel-at-biologie.uni-regensburg.de } [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de] } Sent: Tuesday, November 15, 2016 11:54 AM } To: Marco Moller } Subject: [Microscopy] Pirani } } } } } ---------------------------------------------------------------------- } - } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } - } ----- } } Dear Colleagues, } on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge } is faulty and needs to be replaced (or does anybody know how to } repair? we would need the W wire - can it be replaced? easy? is it W?) . } It is an Edwards PRE10K (D02428000) - and we asked Edwards and they } told us that this Pirani is not available - any more. Hmm. } Does anybody have one for sale which is not used any more? } Does anybody know another type which would fit into the Scancoat Six? } Any help is much appreciated. } kind regards, } Reinhard Rachel } } -- } Prof. Dr. Reinhard Rachel } University of Regensburg } Centre for EM / Anatomy } Faculty of Biology & Preclin. Med. } Universitaetsstrasse 31 } D-93053 Regensburg - Germany } tel +49 941 943 -2837, -1720 } mail reinhard.rachel-at-biologie.uni-regensburg.de } office: VKL 3.1.29 } } Next microscopy conferences: } - Microscopy Conference 2017 (D-A-CH) Lausanne, CH } 20-25 August 2017 } http://www.mc2017.ch/ } - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018 } http://imc19.com/ } - next Microbiol. conferences: } VAAM/DGHM - Annual Conf } March 5-8, 2017, Wuerzburg } } } } } } ==============================Original } Headers============================== } 6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15 } 04:39:13 2016 } 6, 23 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni- } regensburg.de [194.94.155.51]) } 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id uAFAdCBH003403 } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 04:39:13 } -0600 } 6, 23 -- Received: from rrzmta1.uni-regensburg.de (localhost } [127.0.0.1]) } 6, 23 -- by localhost (Postfix) with SMTP id 500835AE01 } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 } +0100 (CET) } 6, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni- } regensburg.de [132.199.5.51]) } 6, 23 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id } 377F45AE3B } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 } +0100 (CET) } 6, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni- } regensburg.de } 6, 23 -- with Novell_GroupWise; Tue, 15 Nov 2016 11:40:33 +0100 } 6, 23 -- Message-Id: {582AF42E020000540006412D-at-gwsmtp1.uni- } regensburg.de} } 6, 23 -- X-Mailer: Novell GroupWise Internet Agent 14.2.1 6, 23 -- } Date: Tue, 15 Nov 2016 11:40:30 +0100 6, 23 -- From: "Reinhard Rachel" } {Reinhard.Rachel-at-biologie.uni- regensburg.de} 6, 23 -- To: "microscopy } server" {Microscopy-at-microscopy.com} 6, 23 -- Subject: Pirani 6, 23 -- } Mime-Version: 1.0 6, 23 -- Content-Type: text/plain; charset=US-ASCII } 6, 23 -- Content-Disposition: inline 6, 23 -- } Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by ns.microscopy.com id uAFAdCBH003403 } ==============================End of - } Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both chrisbrantner-at-gwu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] How to do SEM EDS on embedded teeth
Message: Hi all,
We would like to look at some special teeth samples and to determine the elements in a cross-section sawed out of these samples. The teeth have been embedded in methyl salicylate. The cross sections will be 100 microns thick. Some historical samples have been placed on glass slides and cover slipped or plastic slides and overslipped. I could use some hints about how to obtain an elemental map from this material.
Thanks Chris Brantner George Washington University Nanofabrication and Imaging Center
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Pirani can be repaired if you really wanted to do it, you can DIY after reading some of books and papers on vacuum instrumentation and techniques, or contact some of the places that routinely repair vacuum instrumentation. Examples would be Duniway Stockrom (duniway.com), or VGM Inc. (vgminc.net), or Scientific Instrument Services (sisweb.com) - I am sure there are also plenty of places capable of repairing Pirani in Europe as well, Google is your friend.
Replacement PRE10K also available elsewhere: there are four of these gauges listed on E*Bay, also check Labsource (labsoruce.come) and PLC Center (plccenter.com).
Good luck! Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 11/15/2016 5:40 AM, Reinhard.Rachel-at-biologie.uni-regensburg.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Colleagues, } on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge is faulty and needs to be replaced (or does anybody know how to repair? we would need the W wire - can it be replaced? easy? is it W?) . } It is an Edwards PRE10K (D02428000) - and we asked Edwards and they told us that this Pirani is not available - any more. Hmm. } Does anybody have one for sale which is not used any more? } Does anybody know another type which would fit into the Scancoat Six? } Any help is much appreciated. } kind regards, } Reinhard Rachel }
==============================Original Headers============================== 6, 35 -- From vray-at-partbeamsystech.com Tue Nov 15 12:05:56 2016 6, 35 -- Received: from nm40-vm8.bullet.mail.bf1.yahoo.com (nm40-vm8.bullet.mail.bf1.yahoo.com [72.30.239.216]) 6, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAFI5tkk030124 6, 35 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 12:05:56 -0600 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1479233237; bh=Tg7Qd/QHIHG3cHY5TbbJS6mIZr+5Xs1NyKwjgwdjrTI=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=swGtgORb/zqyolqX4LTykRLKZXII17oG9Aot95NAmu3NiSET2x60uTBY8K0T8hKwRFnK3rU+hp8QBBIu5BEJ2SEOHSWEqZOqe1tas31Ix0Db69eszYNeTQUTB4Yvprt7eBz/4fW1utW3KGWhrDEYzAODgqr56MoMUcheN5BLS3CCOzS08End0/e1R1REu9RHl82uR3ihcZhZBvdm//5QcskPc0X4anKTldDjFqjFzs/CYyjCr664Ax6j+gKAiwOfK0sl5sxSs3+k0Uvj8RPpwneT4DjjPnhOdasFGMcHXX++eLAW8E4Omwezr5aVJz/CaQEFRJhQsa0S6loLUMQUCg== 6, 35 -- Received: from [98.139.170.179] by nm40.bullet.mail.bf1.yahoo.com with NNFMP; 15 Nov 2016 18:07:17 -0000 6, 35 -- Received: from [68.142.230.65] by tm22.bullet.mail.bf1.yahoo.com with NNFMP; 15 Nov 2016 18:07:17 -0000 6, 35 -- Received: from [127.0.0.1] by smtp222.mail.bf1.yahoo.com with NNFMP; 15 Nov 2016 18:07:17 -0000 6, 35 -- X-Yahoo-Newman-Id: 485813.65813.bm-at-smtp222.mail.bf1.yahoo.com 6, 35 -- X-Yahoo-Newman-Property: ymail-3 6, 35 -- X-YMail-OSG: L0dY1t4VM1nNf9hWqU2AvEe8tsuPxdyWqTh3fYv13ublK5l 6, 35 -- gXIG9E8aGsjgomeooRTZl7cZRHx8D3hEgLDLL0BdaLZzZGFnSBTliGRekh8c 6, 35 -- 4fXOq8cAssXRb6Zoh_pW1rEQ3nOtpIG_h5GzKn8BzjTau70ynugequ1VbydJ 6, 35 -- cHNEuq5Dy7.xn9ypUpeobQHRLkjsiG3CqVeP8disqvOnA3LYEGtaatIiICmd 6, 35 -- E52R.XyKXT6iEZWo.46hoLUSBVwLLyqOHXVOFb1a3sxrnRIMvNThekIIkDxW 6, 35 -- WApL0_VlZkzDgdsOKUAt9gQylLM7yklTb66kcTg1e1KTII713354vV5KmDqA 6, 35 -- i7lLJlvhNIc.mVRTMdg4P6U3VoywaFR7ABbqKjzhH8r88Bh33cc1PQW2yJ4g 6, 35 -- kzKnbgrWmwuhOO7YmGHE3vCL0eXwT4af1XJ2l6HdmxyytqbHJdoz41qgJPJh 6, 35 -- aqKQK5MmA.iNnv5KJ7xB6hwQVdfQ6Iw7Wt0fJ8tCHfCnlpwu7hIFUyxkAbgC 6, 35 -- sfEldS.Jd7LgAmYfDMH2r1dRX1rIWMFn8dH0ETvfC6kwcBhsa_tdY5I5TgdZ 6, 35 -- NblO6FQ-- 6, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 35 -- Subject: Re: [Microscopy] Pirani 6, 35 -- To: Reinhard.Rachel-at-biologie.uni-regensburg.de 6, 35 -- References: {201611151040.uAFAeTp0004580-at-ns.microscopy.com} 6, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 6, 35 -- Cc: microscopy-at-microscopy.com 6, 35 -- Message-ID: {e4f0739a-a7dd-1d10-2bb7-3c84ae1afaed-at-partbeamsystech.com} 6, 35 -- Date: Tue, 15 Nov 2016 13:07:16 -0500 6, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 6, 35 -- Thunderbird/45.4.0 6, 35 -- MIME-Version: 1.0 6, 35 -- In-Reply-To: {201611151040.uAFAeTp0004580-at-ns.microscopy.com} 6, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I might suggest checking with Duniway Stockroom Co. They sell and service all kinds of vacuum gauges, and may be able to help you. (www.duniway.com)
-- Wilbur C. Bigelow, PhD, LCDR USNR (Ret) Professor Emeritus of Materials Engineering The University of Michigan 2911 Whittier Court, Ann Arbor MI 48104
} On Nov 15, 2016, at 8:55 AM, mdelann1-at-jhmi.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=lPrq25rbHMXLlcFeGhcyWmtC3t2he12qhEsr-BRMYrU&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=f5l6Y6K4uItHYtsgv0AbcyIF3JS80KNO3F5muWb3x3U&e= } ---------------------------------------------------------------------------- } } Reinhard, } If you can dismantle the gauge and get to the filament you can try } sonicating in mild HCL solution. I do this routinely with my penning gauge } on my FESEM when it gets coated with carbon. } Sincerely, } } Michael Delannoy } } -----Original Message----- } X-from: mmoller-at-cicbiomagune.es [mailto:mmoller-at-cicbiomagune.es] } Sent: Tuesday, November 15, 2016 10:16 AM } To: delannoy-at-jhmi.edu } Subject: [Microscopy] RE: Pirani } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=lPrq25rbHMXLlcFeGhcyWmtC3t2he12qhEsr-BRMYrU&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=f5l6Y6K4uItHYtsgv0AbcyIF3JS80KNO3F5muWb3x3U&e= } ---------------------------------------------------------------------------- } } Hello! } To my knowledge, after Edwards dropped this kind of equipment from their } portfolio, the Indian company HHV continues to market these instruments, and } to sell spare parts for it. I once heard rumors that HHV already before } would have been the place where this Edwards equipment was built. If this } would be true, then contacting HHV would be the way to contact the } manufacturer directly ;-) . There webpage states: "We hold an exclusive } licence from Edwards to manufacture and supply the well-known ScanCoat Six, } Auto306, Auto500 and forensic deposition systems under the HHV brand and } have added our own range of systems to our product line-up." } (https://urldefense.proofpoint.com/v2/url?u=http-3A__www.hhvltd.com_&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=DIWX4B-uub8VIb6xs6hwYO60NH82ro2kq554fy3aXlw&e= ). } Good Luck to find your spare parts there! } Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mvller } } } ---------------------------------------------------------------- } Marco Mvller } Platform Manager - Electron Microscopy } } CIC biomaGUNE } Parque Tecnolsgico de San Sebastian } Paseo Miramsn, 182. Ed. Empresarial C } 20009 San Sebastian (Guipzzcoa) } SPAIN } } Tel: +34 943 00 53 25 } mmoller-at-cicbiomagune.es } } } } -----Original Message----- } } From: Reinhard.Rachel-at-biologie.uni-regensburg.de } } [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de] } } Sent: Tuesday, November 15, 2016 11:54 AM } } To: Marco Moller } } Subject: [Microscopy] Pirani } } } } } } } } } } ---------------------------------------------------------------------- } } - } } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=lPrq25rbHMXLlcFeGhcyWmtC3t2he12qhEsr-BRMYrU&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=f5l6Y6K4uItHYtsgv0AbcyIF3JS80KNO3F5muWb3x3U&e= } } ---------------------------------------------------------------------- } } - } } ----- } } } } Dear Colleagues, } } on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge } } is faulty and needs to be replaced (or does anybody know how to } } repair? we would need the W wire - can it be replaced? easy? is it W?) . } } It is an Edwards PRE10K (D02428000) - and we asked Edwards and they } } told us that this Pirani is not available - any more. Hmm. } } Does anybody have one for sale which is not used any more? } } Does anybody know another type which would fit into the Scancoat Six? } } Any help is much appreciated. } } kind regards, } } Reinhard Rachel } } } } -- } } Prof. Dr. Reinhard Rachel } } University of Regensburg } } Centre for EM / Anatomy } } Faculty of Biology & Preclin. Med. } } Universitaetsstrasse 31 } } D-93053 Regensburg - Germany } } tel +49 941 943 -2837, -1720 } } mail reinhard.rachel-at-biologie.uni-regensburg.de } } office: VKL 3.1.29 } } } } Next microscopy conferences: } } - Microscopy Conference 2017 (D-A-CH) Lausanne, CH } } 20-25 August 2017 } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mc2017.ch_&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=AaD5S11qNvAb-C-mDBQtYtDROq3sFEZ32NS_vLlu0U4&e= } } - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018 } } https://urldefense.proofpoint.com/v2/url?u=http-3A__imc19.com_&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=-jZqy72OCAQpB_QZNwyBrgbkp-5vuH5i6hCtvqvi-oM&e= } } - next Microbiol. conferences: } } VAAM/DGHM - Annual Conf } } March 5-8, 2017, Wuerzburg } } } } } } } } } } } } ==============================Original } } Headers============================== } } 6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15 } } 04:39:13 2016 } } 6, 23 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni- } } regensburg.de [194.94.155.51]) } } 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } } id uAFAdCBH003403 } } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 04:39:13 } } -0600 } } 6, 23 -- Received: from rrzmta1.uni-regensburg.de (localhost } } [127.0.0.1]) } } 6, 23 -- by localhost (Postfix) with SMTP id 500835AE01 } } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 } } +0100 (CET) } } 6, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni- } } regensburg.de [132.199.5.51]) } } 6, 23 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id } } 377F45AE3B } } 6, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2016 11:40:33 } } +0100 (CET) } } 6, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni- } } regensburg.de } } 6, 23 -- with Novell_GroupWise; Tue, 15 Nov 2016 11:40:33 +0100 } } 6, 23 -- Message-Id: {582AF42E020000540006412D-at-gwsmtp1.uni- } } regensburg.de} } } 6, 23 -- X-Mailer: Novell GroupWise Internet Agent 14.2.1 6, 23 -- } } Date: Tue, 15 Nov 2016 11:40:30 +0100 6, 23 -- From: "Reinhard Rachel" } } {Reinhard.Rachel-at-biologie.uni- regensburg.de} 6, 23 -- To: "microscopy } } server" {Microscopy-at-microscopy.com} 6, 23 -- Subject: Pirani 6, 23 -- } } Mime-Version: 1.0 6, 23 -- Content-Type: text/plain; charset=US-ASCII } } 6, 23 -- Content-Disposition: inline 6, 23 -- } } Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com id uAFAdCBH003403 } } ==============================End of - } } Headers============================== } } } ==============================Original Headers============================== } 8, 41 -- From mmoller-at-cicbiomagune.es Tue Nov 15 09:06:21 2016 8, 41 -- } Received: from webmail.cicbiomagune.es (webmail.cicbiomagune.es } [150.241.252.34]) } 8, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } uAFF6KBP000424 } 8, 41 -- for {Microscopy-at-Microscopy.com} ; Tue, 15 Nov 2016 09:06:20 } -0600 } 8, 41 -- X-AuditID: 0a0d0165-1c7ff70000000ce6-af-582b24b8667f } 8, 41 -- Received: from CICMGEXCAS.cicbiomagune.int } (cicmgexcas.cicbiomagune.int [10.10.1.3]) } 8, 41 -- by webmail.cicbiomagune.es (Symantec Messaging Gateway) with
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Organization: University of Missouri-Kansas City School of Dentistry
Title-Subject: [Filtered] Director of Electron Microscopy Laboratory Position
Message: Job Description The University of Missouri-Kansas City, School of Dentistry is seeking a Research Assistant to fill a position as Director of the Electron Microscopy Core Laboratory. The position is full time, 100% benefit eligible.
Minimum Qualifications The successful candidate will have at least 3 years training in electron microscopy (SEM, TEM). A Ph.D. or advanced training is preferred. The candidate should show proficiency in energy dispersive spectroscopy (EDS), have experience in biological specimen preparation for TEM and SEM and familiarity with corresponding equipment (ultramicrotome, knifemaker, CPD, sputter coater) and chemical and immuno-EM procedures. Some experience with materials specimen preparation is a plus. Additionally, the candidate should be able to demonstrate computer literacy and an understanding of digital image processing. Excellent skills in collaborating with multiple faculty and time management are essential.
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We require a recirculating (ductless) fume cupboard for preparation of EM s= pecimens using chemicals such as osmium tetroxide, glutaraldehyde, paraform= aldehyde and epoxy resins. In the past we have used a ducted cupboard, but = this is being decommissioned. Does anyone know what face velocity is needed , or can anyone recommend a suitable cupboard?
Thanks very much
Peter Harris
Dr Peter J F Harris Manager, Electron Microscopy Laboratory JJ Thomson Building | University of Reading | Whiteknights | Reading = | RG6 6AF | UK
==============================Original Headers============================== 7, 32 -- From scsharip-at-reading.ac.uk Wed Nov 16 02:42:41 2016 7, 32 -- Received: from vimg1.rdg.ac.uk (vimg1.rdg.ac.uk [134.225.1.81]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAG8gfB9025073 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Nov 2016 02:42:41 -0600 7, 32 -- Received: from vimh2.rdg.ac.uk ([2001:630:53:20::19e]) 7, 32 -- by vimg1.rdg.ac.uk (Exim: outgoing gateway) 7, 32 -- with esmtp id 1c6vp7-0002EL-0P 7, 32 -- for Microscopy-at-microscopy.com; Wed, 16 Nov 2016 08:44:05 +0000 7, 32 -- Received: from vime-hts2.ipv6.rdg.ac.uk ([2001:630:53:20::10d] helo=vime-hts2.rdg.ac.uk) 7, 32 -- by vimh2.rdg.ac.uk (Exim: virusscanner) 7, 32 -- with esmtps id 1c6vp6-0006MP-Rz 7, 32 -- for Microscopy-at-microscopy.com; Wed, 16 Nov 2016 08:44:04 +0000 7, 32 -- Received: from vime-mbx6.rdg.ac.uk ([fe80::7143:7762:1855:40b]) by 7, 32 -- vime-hts2.rdg.ac.uk ([fe80::d538:4f02:9bfc:999d%10]) with mapi id 7, 32 -- 14.03.0294.000; Wed, 16 Nov 2016 08:44:04 +0000 7, 32 -- From: Peter Harris {p.j.f.harris-at-reading.ac.uk} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Subject: fume cupboard 7, 32 -- Thread-Topic: fume cupboard 7, 32 -- Thread-Index: AdI/5ZV9mpWgm73QQlOqlS5d+EgSXA== 7, 32 -- Date: Wed, 16 Nov 2016 08:44:03 +0000 7, 32 -- Message-ID: {BD66D82094425F40862C7D9FE7644AE3010655B5ED-at-vime-mbx6.rdg.ac.uk} 7, 32 -- Accept-Language: en-GB, en-US 7, 32 -- Content-Language: en-GB 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- x-originating-ip: [134.225.49.100] 7, 32 -- Content-Type: text/plain; charset="us-ascii" 7, 32 -- MIME-Version: 1.0 7, 32 -- X-Scan-Signature: 75b85d49581ee15d224a81c508816dc4 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uAG8gfB9025073 ==============================End of - Headers==============================
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Email: trc-at-tousimis.com Name: TOUSIMIS
Organization: TOUSIMIS Title-Subject: [Filtered] In Memoriam of Dr. Elizabeth Ann Ellis
Message: June 5, 1948 - October 29, 2016
Life Legacy
Dr. Elizabeth Ann Ellis (Ann) passed away peacefully at home Saturday evening, October 29, under the care of Gentiva Hospice. The family is grateful for the care that Gentiva and her wonderful angels from Cairo (Mary, Rutha, and Shirley) provided in her final weeks. Ann was the daughter of Horace W. Ellis and Vivian G. Ellis. Ann was a member of the 1966 graduating class of Thomas County Central High School. Her higher education degrees include a Bachelors degree from the University of Georgia, a Masters degree from the University of North Carolina at Chapel Hill, and a Doctorate from the University of Florida. While studying for her Ph.D., she did research and publication with many ophthalmologists and endocrinologists. As a member of the Southeastern Microscopy Society and Microscopy Society of America, she received numerous awards as a distinguished electron microscopist and outstanding technologist. Dr. Ellis taught electron microscopy research techniques, and did research and published for Texas A&M for 10 years before retiring in 2013. While residing in College Station, Ann was a devoted choirmember and also active as a lay leader at the University of Texas A&M United Methodist Church. Upon returning to Thomasville, Ann became a member of Morningside United Methodist, and also the United Daughters of the Confederacy. Survivors include her mother, Vivian Ellis, her sister, Pamela Ellis, and several cousins.
You will be missed by all that were fortunate enough to get to know you. RIP Ann.
tousimis®
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anybody out there who can send me a PDF copy of the user / installation manual of the IDE MK3 Magnetic Field Cancellation System?
Best,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 18.11.16 um 17:13 schrieb Stefan Diller: } Dear All, } } anybody out there who can send me a PDF copy of the user / installation manual of the IDE MK3 Magnetic Field Cancellation System? } } } Best, } } Stefan } }
==============================Original Headers============================== 11, 25 -- From stefan.diller-at-t-online.de Fri Nov 18 10:16:28 2016 11, 25 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAIGGSWC010342 11, 25 -- for {microscopy-at-microscopy.com} ; Fri, 18 Nov 2016 10:16:28 -0600 11, 25 -- Received: from fwd04.aul.t-online.de (fwd04.aul.t-online.de [172.20.26.149]) 11, 25 -- by mailout08.t-online.de (Postfix) with SMTP id 6889741DAA68 11, 25 -- for {microscopy-at-microscopy.com} ; Fri, 18 Nov 2016 17:18:00 +0100 (CET) 11, 25 -- Received: from mac-pro.local (rAb998ZEZhDvm7SHntk6QNxj3vwjGSIG7lFes04dAQlBAXMJxxRp0x3MQx-tOJvQOo-at-[93.203.83.19]) by fwd04.t-online.de 11, 25 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 11, 25 -- esmtp id 1c7lrO-1PJZq40; Fri, 18 Nov 2016 17:17:54 +0100 11, 25 -- Subject: FOUND: no more need.... IDE MK3 Magnetic Field Cancellation System 11, 25 -- manual needed 11, 25 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 25 -- References: {1aec2f2e-4537-32f4-01de-6df8e046c43a-at-t-online.de} 11, 25 -- From: Stefan Diller {stefan.diller-at-t-online.de} 11, 25 -- Message-ID: {4cce3c05-7a1c-f377-93fe-4564243380b2-at-t-online.de} 11, 25 -- Date: Fri, 18 Nov 2016 17:17:53 +0100 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 11, 25 -- Gecko/20100101 Thunderbird/45.4.0 11, 25 -- MIME-Version: 1.0 11, 25 -- In-Reply-To: {1aec2f2e-4537-32f4-01de-6df8e046c43a-at-t-online.de} 11, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 25 -- Content-Transfer-Encoding: 7bit 11, 25 -- X-ID: rAb998ZEZhDvm7SHntk6QNxj3vwjGSIG7lFes04dAQlBAXMJxxRp0x3MQx-tOJvQOo 11, 25 -- X-TOI-MSGID: 208c8c30-081b-464b-ab4c-a87e4f656a1e ==============================End of - Headers==============================
We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 3 - May 12, 2017.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field, confocal microscopes and new emerging technologies.
Laboratory exercises, demonstrations, and discussions include: - geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; - phase contrast, polarization and interference microscopy; - fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; - principles and application of digital imaging and quantitative digital image deconvolution; - digital image processing and object identification and tracking; - live cell and ratiometric imaging for FRET and ion concentration imaging; - confocal microscopy and specialized methods such as TIRF and FLIM; and - new advances in light microscopy such as FCS, PALM, STORM, SIM and Light Sheet.
The course web site is at http://www.mbl.edu/aqlm.
Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.
Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.
Applications due January 27, 2017.
Directors: Justin Taraska (National Institutes of Health), Jagesh Shah (Harvard Medical School) Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)
Cheers, Jagesh, Justin and Wendy
Visit our Facebook page: http://www.facebook.com/aqlmcourse Follow us on Twitter: -at-BeadsinMay
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility 9 Cambridge Center, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 15, 40 -- From wsalmon-at-wi.mit.edu Mon Nov 21 13:20:08 2016 15, 40 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uALJK86J025108 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2016 13:20:08 -0600 15, 40 -- X-IronPort-AV: E=Sophos;i="5.31,528,1473134400"; 15, 40 -- d="scan'208";a="10272045" 15, 40 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 15, 40 -- by ptolemy.wi.mit.edu with ESMTP; 21 Nov 2016 14:21:52 -0500 15, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 3A8A331E138 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2016 14:21:52 -0500 (EST) 15, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 15, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 15, 40 -- with ESMTP id bVmo7sp5Undw for {microscopy-at-microscopy.com} ; 15, 40 -- Mon, 21 Nov 2016 14:21:51 -0500 (EST) 15, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id AC36331E143 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2016 14:21:51 -0500 (EST) 15, 40 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 15, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 15, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 15, 40 -- with ESMTP id 0DyzDYT4HdI9 for {microscopy-at-microscopy.com} ; 15, 40 -- Mon, 21 Nov 2016 14:21:51 -0500 (EST) 15, 40 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 15, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 922E031E138 15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2016 14:21:51 -0500 (EST) 15, 40 -- Date: Mon, 21 Nov 2016 14:21:51 -0500 (EST) 15, 40 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 15, 40 -- To: microscopy {microscopy-at-microscopy.com} 15, 40 -- Message-ID: {1070074976.5776893.1479756111565.JavaMail.zimbra-at-wi.mit.edu} 15, 40 -- In-Reply-To: {286729935.5776202.1479755760909.JavaMail.zimbra-at-wi.mit.edu} 15, 40 -- References: {286729935.5776202.1479755760909.JavaMail.zimbra-at-wi.mit.edu} 15, 40 -- Subject: Analytical and Quantitative Light Microscopy 2017 15, 40 -- MIME-Version: 1.0 15, 40 -- Content-Type: text/plain; charset=utf-8 15, 40 -- Content-Transfer-Encoding: 7bit 15, 40 -- X-Originating-IP: [10.9.41.94] 15, 40 -- X-Mailer: Zimbra 8.6.0_GA_1191 (ZimbraWebClient - GC54 (Mac)/8.6.0_GA_1191) 15, 40 -- Thread-Topic: Analytical and Quantitative Light Microscopy 2017 15, 40 -- Thread-Index: jr0ckX/tCcFfuC5EqdpvaLSm3Jcnhya+obs8 ==============================End of - Headers==============================
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Email: andrew.johnson-at-uwa.edu.au Name: Andrew Johnson
Organization: University of Western Australia
Title-Subject: [Filtered] JEOL TEM 1011
Message: I have to use the above and would like to see a manual.
Can't find one online so can someone help?
Regards, Andrew
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Hi, Andrew, unfortunately I don't have an official JEOL Manual for the/ their TEM 1011 [personally I have only parts of the JEOL TEM 1010 manuals] but - in case nobody else will offer you a pdf, at least I would like to point you to:
GEORGIA ELECTRON MICROSCOPY JEOL JEM1011 [jeol_jem1011]
The JEOL JEM1011 (JEOL, Inc., Peabody, MA), a 100 kV TEM, is configured for high contrast imaging. It is equipped with an AMT side-mounted digital camera with 2kx2k resolution. Click here* for a basic JEOL 1011 TEM instruction sheet. Advanced Microscopy Techniques, Corp. Woburn, MA * http://web8.ovpr.uga.edu/gem/wp-content/uploads/sites/4/2016/02/JEOL-1011-Gu ide.pdf
But I am sure that at http://www.jeol.co.jp/au/ you'll find relevant Contact Data to perhaps apply for a manual you're in need: JEOL (AUSTRALASIA) PTY. LTD. SALES & SERVICE
Suite 1, Level 2, 18 Aquatic Drive Frenchs Forest NSW 2086 Australia TEL: 02-9451-3855 FAX: 02-9451-3822 Email: info-at-jeol.com.au
Disclaimer: no affiliation, no financial interest. ============================================================================ =======
-----Ursprngliche Nachricht----- Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Dienstag, 22. November 2016 21:35 An: wij.muss-at-aon.at Betreff: [Microscopy] JEOL TEM 1011 Manual Needed
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I have to use the above and would like to see a manual. Can't find one online so can someone help?
Regards, Andrew
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Dear All, Our AFM training course is taking place again in beautiful Porto, Portugal between 10th and the 13th April 2017. This is the fifth edition of the course, which contains a mixture, of background, tips and tricks, sample prep., hands-on instrument training, and data analysis. The focus of the course is always on the practical aspects of AFM. As usual, we will also have some external experts come in to talk about advanced topics in materials and biological areas. For more details of the course, visit: afmhelp.com/course Best Regards, Peter Eaton
____________________________________________________________________________ Atomic Force Microscopy Dr Peter Eaton and Dr Paul West Available through all good bookshops, or direct from Oxford University Press at: http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
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Email: vakimler-at-oakland.edu Name: Vickie Anna Kimler
Organization: Ocular Structure and Imaging Facility - Eye Research Institute
Message: When the grid with a protein polymer is in the scope, should it be placed filament side up, or filament side down for the sharpest digital pictures? The samples are small biopolymers in different conformations. They are negatively stained with ethanolic 2% UA2. Thanks, Vickie
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Hello Vickie, I was taught, a long time ago, that the sample should be "placed filament side up". Did you consider to use carbon film grids for your samples? Negative staining on thin carbon support film should result in better signal-to-noise ratio ("sharpest digital picture").
Best regards
Oldrich
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 10, 47 -- From benada-at-biomed.cas.cz Tue Nov 29 01:53:57 2016 10, 47 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 10, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAT7rvGA015924 10, 47 -- for {microscopy-at-microscopy.com} ; Tue, 29 Nov 2016 01:53:57 -0600 10, 47 -- X-ASG-Debug-ID: 1480406166-05011e1397337cb0001-4CH8be 10, 47 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id jMNoqPCKA1wwmqLt; Tue, 29 Nov 2016 08:56:06 +0100 (CET) 10, 47 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 10, 47 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 10, 47 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 10, 47 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 10, 47 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 10, 47 -- (No client certificate requested) 10, 47 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id B3F0DD002FF; 10, 47 -- Tue, 29 Nov 2016 08:56:02 +0100 (CET) 10, 47 -- Date: Tue, 29 Nov 2016 08:56:01 +0100 10, 47 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 47 -- To: Microscopy-at-microscopy.com, vakimler-at-oakland.edu 10, 47 -- Subject: Re: [Microscopy] viaWWW:Grid Placement in Negatively Stained 10, 47 -- Formvar-Carbon Mounted 10, 47 -- Message-ID: {20161129085601.15f0d831-at-u117ob02} 10, 47 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW:Grid Placement in Negatively Stained 10, 47 -- Formvar-Carbon Mounted 10, 47 -- In-Reply-To: {201611282259.uASMxdlD014571-at-ns.microscopy.com} 10, 47 -- References: {201611282259.uASMxdlD014571-at-ns.microscopy.com} 10, 47 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 47 -- =?UTF-8?B?xIxS?= 10, 47 -- X-Mailer: Claws Mail 3.14.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 10, 47 -- MIME-Version: 1.0 10, 47 -- Content-Type: text/plain; charset=UTF-8 10, 47 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 47 -- X-IoP-CAS-MailScanner-ID: B3F0DD002FF.A9C75 10, 47 -- X-IoP-CAS-MailScanner: Processed 10, 47 -- X-Spam-Status: No 10, 47 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 10, 47 -- X-Barracuda-Start-Time: 1480406166 10, 47 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 10, 47 -- X-Barracuda-Scan-Msg-Size: 3106 10, 47 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 10, 47 -- X-Barracuda-BRTS-Status: 1 10, 47 -- X-Barracuda-Spam-Score: 0.00 10, 47 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests= 10, 47 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.34798 10, 47 -- Rule breakdown below 10, 47 -- pts rule name description 10, 47 -- ---- ---------------------- -------------------------------------------------- 10, 47 -- Content-Transfer-Encoding: 8bit 10, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uAT7rvGA015924 ==============================End of - Headers==============================
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Email: wentao0108-at-gmail.com Name: Wentao Qin
Organization: On Semiconductor
Title-Subject: [Filtered] TEM Ion Mills
Message: Which brand of ion mill, in addition to Gatan's PIPS, for TEM sample preparation is good - I would appreciate your input! How about "Technoorg Linda"?
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After 40+ years of paper notebooks I converted to a full electronic notebook about 4 years ago.
I use an iPad Pro with a program called NotesPlus (Apple Store). You can type via keyboard, draw or write using a stylus, as well as capture photos and store them all in the notebook. It can import PDFs, and for me importantly it can export the notebook or pages to a PDF file on a server which you than then archive and/or share with colleagues. Fits my operation mode beautiflully as I can store the experimental note with the data all in an archive.
The program is extremely cheap { $20, but it only runs on an iPad.
Disclaimer: I have no commercial connections with either companies, but wish I did.
Nestor Your Friendly Neighborhood SysOp
} On Nov 28, 2016, at 6:10 PM CST, jkrupp-at-deltacollege.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Any one using electronic lab notebooks? } } Any comments/remarks on pros/cons. } } Jon } } Jonathan Krupp } ASB&T } San Joaquin Delta College } 5151Pacific Ave. } Stockton, CA 95207 } (209) 954-5284 } jkrupp-at-deltacollege.edu } }
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
The Electron Microscopy Service in the Research Resources Center at UIC is looking for an EM Technologist. Under general supervision from the Director of Research Service Facility (EMS), he/she will perform duties involved in the preparation of life science specimens for observation on scanning and transmission electron microscopes. He/She will also handle record keeping and maintenance, with an emphasis on processing samples for researchers, and also in the day to day running of the Electron Microscopes.
For more information or to apply (by Monday 5th December) go to:-
-- Alan W Nicholls, PhD Associate Director, RRC Director Research Resource Facility - Electron Microscopy Research Resources Center, University of Illinois at Chicago Rm 110, 845 West Taylor St Chicago, IL 60607 312 996 1227 nicholls-at-uic.edu
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Dear Fellow Microscopists, Is it safe for a TEM microscope to image a holey C film dispersed with thousands of Fe3O4 magnetite particles (100nm)? The specimen is prepared in the usual way as many other nanoparticles specimens. Will the particles fly to the pole pieces? The user said the particles are iron oxide and are superparamagnetic (and have no permanent magnetisation). I don't quite understand these terms, but by diffraction I know it fits magnetite Fe3O4. If it's not safe what solutions are there to get the job done? I would be very grateful if you can share with me your experience and comments. Zhou
Dr Z Zhou Research Fellow Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
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Thank you very much for all the help I got in 20+ years that I am a member of the Listserver! For first 20+ years of my microscopy work I was in materials/metals field, but then found myself more and more drifting toward biomedical research. Surprisingly my transition was rather smooth. I believe it was mostly because of the great help I was getting from you, dear Listers.
Now I am retiring, but hope my experience could be helpful for somebody. I do not unsubscribe.
Thank you again,
Vladimir
P.S. In the last few years I mostly stopped replaying on questions posted on Listserver. I got tired (or I did not have time) to clean my mailbox from dozens of "out of office" messages. Now I will have more time, of course. But please, do not make this place usable only for retirees, who do have spare time. Please, find a way not to send these horrible "out of office" messages to Listserver.
P.P.S. And special thanks to Nestor.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director (816) 235-2072 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
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I know its alarming, but yes, it is in general safe. I have looked at magnetite nanoparticles at both HP and at OSU in a JEOL 2500 and Titan TEM respectively without serious issues. They key is they need to be nanoparticles. I believe Steve Chapman and Protrain wrote a nice reply to a similar question 4 or 5 years ago. In that reply Steve mentioned that the Van der Waals force is so large on a nanoparticle they really stick well to the carbon film! I agree, I have seldom seen one fly up. However, as a precaution, I blow a duster can or dry nitrogen gas over my grids before putting any nanoparticles in the TEM (suggestion from Debby Sherman of Purdue). This works very well to dislodge any nanoparticles that are not well adhered. Then as you magnify up on the nanoparticles, halt the analysis if they begin to move!
Pete Eschbach Oregon State University EM Facility 541 737 5645
On 11/30/16, 3:38 AM, "Z.Zhou-at-lboro.ac.uk" {Z.Zhou-at-lboro.ac.uk} wrote:
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Dear Fellow Microscopists, Is it safe for a TEM microscope to image a holey C film dispersed with thousands of Fe3O4 magnetite particles (100nm)? The specimen is prepared in the usual way as many other nanoparticles specimens. Will the particles fly to the pole pieces? The user said the particles are iron oxide and are superparamagnetic (and have no permanent magnetisation). I don't quite understand these terms, but by diffraction I know it fits magnetite Fe3O4. If it's not safe what solutions are there to get the job done? I would be very grateful if you can share with me your experience and comments. Zhou
Dr Z Zhou Research Fellow Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
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6, 67 -- Thread-Topic: TEM imaging Fe3O4 Magnetite particles, is it safe for the scope? 6, 67 -- Thread-Index: AdJK+znsxsY5gfXQQFGv7z6khc9YIg== 6, 67 -- Date: Wed, 30 Nov 2016 11:17:55 +0000 6, 67 -- Message-ID: {AM5PR0402MB2771759A209BDA1CAC79278FC68C0-at-AM5PR0402MB2771.eurprd04.prod.outlook.com} 6, 67 -- Accept-Language: en-GB, en-US 6, 67 -- Content-Language: en-US 6, 67 -- X-MS-Has-Attach: 6, 67 -- X-MS-TNEF-Correlator: 6, 67 -- authentication-results: spf=none (sender IP is ) 6, 67 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; 6, 67 -- x-originating-ip: [2001:630:301:3064:9513:9f70:d61d:d558] 6, 67 -- x-ms-office365-filtering-correlation-id: 713e6e37-eb8b-487e-c589-08d4191288c6 6, 67 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:AM5PR0402MB2771; 6, 67 -- x-microsoft-exchange-diagnostics: 1;AM5PR0402MB2771;7:NhH+M2O3o1IzGf/Gd6IcfG+SsL7dVr3bU2/0HeJkocq+GqaPcB65qGzaRD20UHVixWrAUON2/0fre8jAYjtr98EaplAf6T08zL4g9Grlnjah764Vgyqi0llP39Qi9rh08zC/QT1Ekf0yPke/9gkOAPTK0jmOHOoUpKn8agzM++duZuj50vmkEGtLZrJKdW1LXXpQXnl4Iv+wSpoKZtUYJGGyGoDPZs4FZrL1WP1vNcys69j5+lw0MhuyqFjj2oYIZBKH2GvQ8cdBKs46NbzzaJeoYb2SHvVYb9aJxUaSxgLN7UjqJhVIJPbp5IHBVPdWmPNHyF2VBi6kGWEGj0Kzc7qez6XsXFHZptXP5sxp3TGmT4UyE98ehbdRiXqVxwrzfMuinGdCDeM80D5sln4roMiCneakWtjCBKewkxEOPZ8xpTguA3tpRVVOK70VyPc7kl7Y38yQA3E5VzE6CRz0Ag== 6, 67 -- x-microsoft-antispam-prvs: {AM5PR0402MB2771E18D2D4DC5E8E929ACF8C68C0-at-AM5PR0402MB2771.eurprd04.prod.outlook.com} 6, 67 -- x-exchange-antispam-report-test: UriScan:; 6, 67 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(5005006)(8121501046)(3002001)(10201501046)(6041248)(20161123558021)(20161123564025)(20161123555025)(20161123560025)(20161123562025)(6072148)(6047074);SRVR:AM5PR0402MB2771;BCL:0;PCL:0;RULEID:;SRVR:AM5PR0402MB2771; 6, 67 -- x-forefront-prvs: 0142F22657 6, 67 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(7916002)(199003)(189002)(23363002)(50986999)(2900100001)(92566002)(86362001)(450100001)(76576001)(74482002)(38730400001)(6506003)(122556002)(189998001)(74316002)(97736004)(39410400001)(39450400002)(77096006)(107886002)(110136003)(305945005)(7846002)(7696004)(7736002)(6916009)(42882006)(68736007)(105586002)(5640700001)(3660700001)(81166006)(9686002)(2501003)(8936002)(101416001)(2906002)(5660300001)(81156014)(33656002)(102836003)(6116002)(106356001)(54356999)(3280700002)(2351001)(8676002);DIR:OUT;SFP:1102;SCL:1;SRVR:AM5PR0402MB2771;H:AM5PR0402MB2771.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 6, 67 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate 6, 67 -- permitted sender hosts) 6, 67 -- spamdiagnosticoutput: 1:99 6, 67 -- spamdiagnosticmetadata: NSPM 6, 67 -- Content-Type: text/plain; charset="utf-8" 6, 67 -- MIME-Version: 1.0 6, 67 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 30 Nov 2016 11:17:55.8274 6, 67 -- (UTC) 6, 67 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 6, 67 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b 6, 67 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: AM5PR0402MB2771 6, 67 -- X-OriginatorOrg: lboro.ac.uk 6, 67 -- X-Scan-Signature: 13884a2d58f8f4bc7e50ef16bad8e2dd 6, 67 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 6, 67 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 30 Nov 2016 11:17:57 +0000 6, 67 -- Content-Transfer-Encoding: 8bit 6, 67 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id uAUBFuYX003610 ==============================End of - Headers==============================
==============================Original Headers============================== 8, 42 -- From Peter.Eschbach-at-oregonstate.edu Wed Nov 30 11:44:53 2016 8, 42 -- Received: from smtp2.nws.oregonstate.edu (smtp2.nws.oregonstate.edu [128.193.15.45]) 8, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAUHirZb032764 8, 42 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2016 11:44:53 -0600 8, 42 -- Received: from localhost (localhost [127.0.0.1]) 8, 42 -- by smtp2.nws.oregonstate.edu (Postfix) with ESMTP id 761823446E7; 8, 42 -- Wed, 30 Nov 2016 09:47:09 -0800 (PST) 8, 42 -- X-Virus-Scanned: Debian amavisd-new at relay1.nws.oregonstate.edu 8, 42 -- Received: from smtp2.nws.oregonstate.edu ([127.0.0.1]) 8, 42 -- by localhost (smtp2.nws.oregonstate.edu [127.0.0.1]) (amavisd-new, port 10024) 8, 42 -- with ESMTP id dfeLmx4YM-UP; Wed, 30 Nov 2016 09:47:09 -0800 (PST) 8, 42 -- Received: from EX2.oregonstate.edu (ex2.oregonstate.edu [128.193.7.77]) 8, 42 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 8, 42 -- (No client certificate requested) 8, 42 -- by smtp2.nws.oregonstate.edu (Postfix) with ESMTPS id 52C0C343D29; 8, 42 -- Wed, 30 Nov 2016 09:47:09 -0800 (PST) 8, 42 -- Received: from EX3.oregonstate.edu ([169.254.3.19]) by EX2.oregonstate.edu 8, 42 -- ([169.254.2.245]) with mapi id 14.03.0301.000; Wed, 30 Nov 2016 09:47:08 8, 42 -- -0800 8, 42 -- From: "Eschbach, Peter" {Peter.Eschbach-at-oregonstate.edu} 8, 42 -- To: "Z.Zhou-at-lboro.ac.uk" {Z.Zhou-at-lboro.ac.uk} 8, 42 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 42 -- Subject: Re: [Microscopy] TEM imaging Fe3O4 Magnetite particles, is it safe 8, 42 -- for the scope? 8, 42 -- Thread-Topic: [Microscopy] TEM imaging Fe3O4 Magnetite particles, is it safe 8, 42 -- for the scope? 8, 42 -- Thread-Index: AQHSSv6ex6bfJOxPdU2R7lJEgu7ep6DxzZEA 8, 42 -- Date: Wed, 30 Nov 2016 17:47:07 +0000 8, 42 -- Message-ID: {A6B148FE-3786-439F-B5C3-EB4D4D92E74C-at-oregonstate.edu} 8, 42 -- References: {201611301138.uAUBceKe027664-at-ns.microscopy.com} 8, 42 -- In-Reply-To: {201611301138.uAUBceKe027664-at-ns.microscopy.com} 8, 42 -- Accept-Language: en-US 8, 42 -- Content-Language: en-US 8, 42 -- X-MS-Has-Attach: 8, 42 -- X-MS-TNEF-Correlator: 8, 42 -- user-agent: Microsoft-MacOutlook/f.1c.1.161117 8, 42 -- x-originating-ip: [128.193.7.75] 8, 42 -- Content-Type: text/plain; charset="utf-8" 8, 42 -- Content-ID: {CBF5AE353520014BA443191A4BE982D5-at-oregonstate.edu} 8, 42 -- MIME-Version: 1.0 8, 42 -- Content-Transfer-Encoding: 8bit 8, 42 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id uAUHirZb032764 ==============================End of - Headers==============================
Post Doc First Place: Dr Bhushan Mahadik - "A Predictive Computational Model of Hematopoietic Stem Cell Differentiation Kinetics in Culture”
Post Doc Second Place: Dr Pilgyu Kang - "Flexible and Wearable Optoelectronic Sensors for BiomedicalTechnologies: Crumpled Graphene Stretchable Photodetector”
Student First Place: Fatemeh Ostadhossein - "Antimicrobial and Diagnostic Feasibility of Inherently Therapeutic Nano-Hafnium for Periodontal Disease”
Student Second Place: Aaro Schwartz-Duval - "Towards a Cell-level Personalization of Nanomedicine: Pathology Dependent In Vitro Reduction of Gold Nanoparticles by Action of Mammalian Cells."
The MRL Fall Student/Post Doc Presentation is open to all in Academia, not just Illinois. Plans for a possible 2 Category Competition next year, one day biological, the second Material.
Stay tuned in the spring for announcements of this opportunity!
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
I am trying to make carbon film replicas of particulates on a surface. After lifting the particles off the surface with standard cellulose acetate replicating tape, I carbon coat the tape and use a condensation washer to wash away the tape.
So far, I haven't been able to maintain an intact carbon film through the process even after going to 80nm thick films. The only major difference between my current procedure and what I did many years ago is that I'm using a turbo-pumped thread coater rather than a diffusion-pumped carbon arc deposition unit. Both systems are pulling down to about the same pressure before the deposition.
Is there a difference in strength between a carbon film from thread and one from a carbon arc? There is obviously a significant amount of stress generated since the cellulose acetate swells as it begins to dissolve.
Any suggestions?
Thanks in advance, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
Dear Listers, has anyone experience with replacing the diamond wire in a wire saw? Yesterday my wire broke, but not far from one end. Is it possibile to use a shorter wire than the replacement provided by sellers? My idea was to re-use the longest part of the broken wire, but I wonder if the wire motor needs a the full lenght wire to work correctly. Any comment will be appreciated. Thanks
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Email: abdulkhaliq.khan-at-umanitoba.ca Name: Abdul Khan
I have a strange issue with my JEOL 2100F. The panel subsystem and gonio subsystem on status Monitor show error message intermittently. During error message both Left and Right panels do not work.The error message goes away itself after about 10 minutes and then comes back again after about 10-12 minutes. This keeps on happening all the time. The system is not on service contract. Any suggestions from the microscopy community or from Ex service guys are welcome.
Thank You
Abdul Khan Electron Microscopy Facility Manager Department of Mechanical Engineering University of Manitoba, Canada Ph; 2043337881
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM workshop
Message: March 8-10, 2017, 8am-5pm each day.
This intensive, three-day workshop will provide a practical and basic theoretical introduction to Transmission Electron Microscopy and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment.
Cost: UGA: $600 per participant, Non-Profit Institutions: $800 per participant, For-Profit participants: $1200. This fee includes supplies, chemicals, hands-on beam time, personalized training by GEM staff, and completion certificate. Lunch is provided each day.
Where: GEM laboratories, Barrow Hall, UGA Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account.
Instructors:
John Shields, Ph.D. Dr. Shields is the Managing Director of GEM. He has over 25 years experience with electron microscopy, analysis, and confocal microscopy in various disciplines. While his main work is with biological samples, he has extensive experience with geology, food science, textiles and archaeology.
Mary Ard Ms Ard has over 30 years experience in electron microscopy. She is also a board-certified Histotechnician. Ms Ard works primarily with animal pathologens for Veterinary Pathology, but has a wide range of experience with cell biology and microbiological specimens.
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Adding to the nice comments that Peter made, you are able to reduce the level of magnetic interference with any "uncomfortable" material, by simply extending the objective lens focal length through adjusting your eucentric stage to its lowest position. Cranking the z' so that you are turning your focus controls anticlockwise will reduce the active objective lens current. The magnification will drop, as will the level of resolution achievable. For those who want a little more resolution the trick is to take the z' the other way, shortening the focal length, with the increase in the operating lens current serving to reduce the aberrations and increase resolving power.
Enjoy
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
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Zhou:
I know its alarming, but yes, it is in general safe. I have looked at magnetite nanoparticles at both HP and at OSU in a JEOL 2500 and Titan TEM respectively without serious issues. They key is they need to be nanoparticles. I believe Steve Chapman and Protrain wrote a nice reply to a similar question 4 or 5 years ago. In that reply Steve mentioned that the Van der Waals force is so large on a nanoparticle they really stick well to the carbon film! I agree, I have seldom seen one fly up. However, as a precaution, I blow a duster can or dry nitrogen gas over my grids before putting any nanoparticles in the TEM (suggestion from Debby Sherman of Purdue). This works very well to dislodge any nanoparticles that are not well adhered. Then as you magnify up on the nanoparticles, halt the analysis if they begin to move!
Pete Eschbach Oregon State University EM Facility 541 737 5645
On 11/30/16, 3:38 AM, "Z.Zhou-at-lboro.ac.uk" {Z.Zhou-at-lboro.ac.uk} wrote:
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Dear Fellow Microscopists, Is it safe for a TEM microscope to image a holey C film dispersed with thousands of Fe3O4 magnetite particles (100nm)? The specimen is prepared in the usual way as many other nanoparticles specimens. Will the particles fly to the pole pieces? The user said the particles are iron oxide and are superparamagnetic (and have no permanent magnetisation). I don't quite understand these terms, but by diffraction I know it fits magnetite Fe3O4. If it's not safe what solutions are there to get the job done? I would be very grateful if you can share with me your experience and comments. Zhou
Dr Z Zhou Research Fellow Loughborough Materials Characterisation Centre (LMCC) Department of Materials Loughborough University Leicestershire LE11 3TU
Tel: 01509 223163 Fax: 01509 234225
==============================Original Headers============================== 6, 67 -- From Z.Zhou-at-lboro.ac.uk Wed Nov 30 05:15:57 2016 6, 67 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 6, 67 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uAUBFuYX003610 6, 67 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2016 05:15:56 -0600 6, 67 -- Received: from [158.125.160.17] (helo=itsex-2.lunet.lboro.ac.uk) 6, 67 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) 6, 67 -- (Exim 4.86) 6, 67 -- id 1cC2th-0007n5-8W 6, 67 -- for Microscopy-at-microscopy.com; Wed, 30 Nov 2016 11:17:57 +0000 6, 67 -- Received: from itsex-2.lunet.lboro.ac.uk 6, 67 -- (2001:630:301:d314:580f:144d:cf5e:c130) by itsex-2.lunet.lboro.ac.uk 6, 67 -- (2001:630:301:d314:580f:144d:cf5e:c130) with Microsoft SMTP Server (TLS) id 6, 67 -- 15.1.466.34; Wed, 30 Nov 2016 11:17:56 +0000 6, 67 -- Received: from EUR03-DB5-obe.outbound.protection.outlook.com (94.245.120.85) 6, 67 -- by itsex-2.lunet.lboro.ac.uk (158.125.160.17) with Microsoft SMTP Server 6, 67 -- (TLS) id 15.1.466.34 via Frontend Transport; Wed, 30 Nov 2016 11:17:56 +0000 6, 67 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; 6, 67 -- s=selector1-lboro-ac-uk; 6, 67 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 6, 67 -- bh=jKnrp8qC9hiMMD6JoVyMJc9QB2va7nK+9LGNCH64whE=; 6, 67 -- b=fXEhWzkSXHMMdABvWufyW2VZMpouvejzav97NHv8zxXRkTP0VxvgMUIzNtaa+GiZe6PUwovEuf xghyl4EwiXKqJilPQyjQ5Cacc8+vmcdvOT3eU5Nb+r9SGTkngIC9Ae5doHBA+7Jj0Fx7BSCAHA1f hwM7f4H3gybrKHxBvqnyc= 6, 67 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) by 6, 67 -- AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) with
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Email: gary-at-microtechnics.com Name: Dr Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] Gas filled storage container
Message: Hi All:
I'm looking for a smallish storage container for SEM tubes and mounted specimens that I can fill/purge with gas. This is opposite of a vacuum container. Something less than a cubic foot would be a good size.
Any ideas or sources?
gary g.
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Small gas-purged box is very easy and quick to make.
Pick "airtight" or "weather resistant" storage box of your liking in your local hardware store or online. Make sure box has a breather valve to discharge excess pressure. Pick a fitting or quick-disconnect and some 1/4" or 6mm tubing at the same place. Drill a hole in the box, attach tube through fitting or quick-disconnect, and connect to the purge gas supply.
Most of airtight boxes would come with a breather valve already installed, but if not then you can get a valve on E*Bay or order here: http://www.agmcontainer.com/breather_valves
Happy purging! Valery :)
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
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==============================Original Headers============================== 7, 35 -- From vray-at-partbeamsystech.com Sat Dec 3 14:45:23 2016 7, 35 -- Received: from nm37-vm4.bullet.mail.bf1.yahoo.com (nm37-vm4.bullet.mail.bf1.yahoo.com [72.30.238.204]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uB3KjMk1025306 7, 35 -- for {microscopy-at-microscopy.com} ; Sat, 3 Dec 2016 14:45:23 -0600 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1480798070; bh=fwV4vpqUHO9Jl/MLnvCfxUg25EBD3QBdpyGv1birAu8=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=TJXB2cl8a69a+TJ7De+Cr20XK18Ua+Ryx0U8jvMMOU44Gg/fjr3HkyoTj61fftAgNxmrilOjW2J1jrRmBe0xavBgZO8tEi4NHxdYu79QBdMoVaIg0Bpj+pE3cX8+fvNo4uunID99iJc1/z6pYlfIMZvQlcBUUF/uOz5AYZUUgCl/NBGTcePTJnQF7is0sawCiBawtVqKae5Tmamwnuh4v08MQ34hpRo8Lu2ZtCgHFlY/LnbUGb8Puw0sjtZ7zYDkxv8NFT6rKs7mCv64k3SEOB1FDfpxStawj+OLulJEKcwWy/19rdOG3alA9J2KS9XR0pgGWWe8MvkzifAHFZbvEw== 7, 35 -- Received: from [98.139.170.181] by nm37.bullet.mail.bf1.yahoo.com with NNFMP; 03 Dec 2016 20:47:50 -0000 7, 35 -- Received: from [68.142.230.72] by tm24.bullet.mail.bf1.yahoo.com with NNFMP; 03 Dec 2016 20:47:50 -0000 7, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 03 Dec 2016 20:47:50 -0000 7, 35 -- X-Yahoo-Newman-Id: 479028.13075.bm-at-smtp229.mail.bf1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: F0D8DF8VM1mbDHuPzn3flGQReUyJ28QYgJd0YTHokjE9dci 7, 35 -- VHanFwvWjyBe9JZqDeNQF7vH.R5CzBEH_BsyIclBipOsUP0lfj.aFSAmIRTs 7, 35 -- UsOxifDkAAsefvspKOK0cM9gjD1imH7zSWj1G2ioBDfIAeXiZoWQ.SJIxOnf 7, 35 -- ac9EFwCtjFVenspJdLWgqYB65pTB8Z8FUuba7XwrJXk.KfAF0_6iaroGJ3um 7, 35 -- Dv28sBNFcBzNGFMK6130NzdKL_rcGrvn98HU0eAQz2X2qG2posce856PFqEs 7, 35 -- S74mFsCVFzcd1Jq9l07JQv9JAZcbwIJbU6BXCDWz_tXoZxCPNu8N7AoHdlR1 7, 35 -- PRXbw5dpSEaZ8hREi.dbU8F2yC7OTcgrcP9mxw1IUg3gw8oBSn1s7co6cUfF 7, 35 -- PYJ.wX2slQo4ImU0Ajn9kmr.EjSuxNM_eSllTnFDRIiWXIf4mwZ9w7gZz1mY 7, 35 -- QqEXxr4L7MxhVWaHNM5eKhAgqEFDJRFtP5Dr4CECVOV1LxY8zm_NNzusOa_2 7, 35 -- xaEgZ42CpX2JQ9TSDP5HUlcY1mwv5AnSRdI2Rt_u_1Wc9y8P7VLm0sYDKZDK 7, 35 -- ItFRVCQ-- 7, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 35 -- Subject: Re: [Microscopy] viaWWW:Gas filled storage container 7, 35 -- To: gary-at-microtechnics.com 7, 35 -- References: {201612031752.uB3HqEBT029679-at-ns.microscopy.com} 7, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 35 -- Cc: microscopy-at-microscopy.com 7, 35 -- Message-ID: {eac66021-4152-f004-45c8-1230fbb2552c-at-partbeamsystech.com} 7, 35 -- Date: Sat, 3 Dec 2016 15:47:19 -0500 7, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 7, 35 -- Thunderbird/45.5.1 7, 35 -- MIME-Version: 1.0 7, 35 -- In-Reply-To: {201612031752.uB3HqEBT029679-at-ns.microscopy.com} 7, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I repurposed an old glass desiccator that had a pump-out port in the lid as a low-O2 storage unit. Basically, I stuck a styrofoam coffee cup inside and filled it with LN2 and left the pumping port open to vent the boil-off. I figure that the N2 is generally going to be colder than the residual atmosphere and force the warmer air out the port. When the LN2 has evaporated, I just close the valve.
We had some people put SEM mounts that were in the air-tight storage tubes in the desiccator. I'm not sure how much good that did!
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
The Electron Microscopy Facility of the Vienna Biocenter Core Facilities (Vienna, Austria) and Nexperion - Solutions for Electron Microscopy (Vienna, Austria) are pleased to announce a jointly organised international
Advanced Workshop on Cryo-Electron Tomography Vienna, Austria, Europe May 6/7, 2017: Optional Pre-Course May 8–12, 2017: Workshop
for students, post-doctoral staff, scientists, and group leaders from academia and industry.
While this workshop targets an advanced audience wit pre-existing knowledge in electron tomography, data processing and/or Cryo-TEM, a weekend pre-course will be offered for less experienced participants to catch-up.
The main part of the workshop will to cover
Immersion freezing for cryo-electron tomography High pressure freezing and CEMOVIS Cryo-CLEM Low-dose data collection with SerialEM Direct electron detectors Cryo-STEM tomography Processing of low-dose tilt series with IMOD Modelling and interpretation of cryo-electron tomography data Subtomogram averaging with PEET
Prospective Instructors include
Dr. Mikhail Eltsov, Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Germany Dr. Thomas Heuser, Vienna Biocenter Core Facilities, Vienna, Austria Dr. Johanna Höög, University of Gothenburg, Sweden Dr. David Mastronarde, University of Colorado, Boulder, United States Dr. Reinhard Rachel, University of Regensburg, Germany Dr. Guenter Resch, Nexperion - Solutions for Electron Microscopy, Vienna, Austria Dr. Sharon Grayer Wolf, Weizmann Institute of Science, Rehovot, Israel
For further details about venue and on-site infrastructure, the registration procedure (deadline: January 31, 2017), and fees, please visit
https://www.nexperion.net/cryotomo2017
We are looking forward to meeting you in Vienna,
Guenter Resch Thomas Heuser
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
==============================Original Headers============================== 8, 22 -- From lists-at-nexperion.net Sun Dec 4 15:32:22 2016 8, 22 -- Received: from 503.hosttech.eu (nexperion.net [62.112.145.34]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uB4LWLwf011084 8, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 4 Dec 2016 15:32:22 -0600 8, 22 -- Received: from 503.hosttech.eu (localhost [127.0.0.1]) 8, 22 -- by 503.hosttech.eu (Postfix) with ESMTP id 1B8CF6D8E2E 8, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 4 Dec 2016 22:34:52 +0100 (CET) 8, 22 -- Received: from vortex (p57bfb401.dip0.t-ipconnect.de [87.191.180.1]) 8, 22 -- by 503.hosttech.eu (Postfix) with ESMTPSA id EB8AE6D8E2D 8, 22 -- for {Microscopy-at-microscopy.com} ; Sun, 4 Dec 2016 22:34:51 +0100 (CET) 8, 22 -- Date: Sun, 4 Dec 2016 22:34:51 +0100 8, 22 -- From: Guenter Resch {lists-at-nexperion.net} 8, 22 -- To: Microscopy-at-microscopy.com 8, 22 -- Subject: Advanced Workshop on Cryo-Electron Tomography, Vienna, Austria, May 8, 22 -- 2017 8, 22 -- Message-ID: {20161204223451.65f61a9b-at-vortex} 8, 22 -- X-Mailer: Claws Mail 3.14.0 (GTK+ 2.24.30; x86_64-pc-linux-gnu) 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: text/plain; charset=UTF-8 8, 22 -- X-Virus-Scanned: ClamAV using ClamSMTP 8, 22 -- Content-Transfer-Encoding: 8bit 8, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uB4LWLwf011084 ==============================End of - Headers==============================
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, December 03, 2016 1:12 PM To: Richard A. Ross
X-from: gary-at-microtechnics.com
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Email: gary-at-microtechnics.com Name: Dr Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] Gas filled storage container
Message: Hi All:
I'm looking for a smallish storage container for SEM tubes and mounted specimens that I can fill/purge with gas. This is opposite of a vacuum container. Something less than a cubic foot would be a good size.
Any ideas or sources?
gary g.
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If you haven't already done so, please consider joining us for EMAS 2017 - 15th European Workshop on Modern Developments and Applications in Microbeam Analysis and IUMAS-7 Meeting at the Bodensee in Konstanz, Germany from May 7th to 11th 2017.
Early bird registration for this event ends on March 15th 2017. The deadline for abstract submissions is February 28th 2017.
All details and on-line registration/abstract submission are available at:
http://www.microbeamanalysis.eu/
Best wishes Philipp
==============================Original Headers============================== 11, 29 -- From Philipp.POEML-at-ec.europa.eu Wed Dec 7 08:16:16 2016 11, 29 -- Received: from out.mail.ec.europa.eu ([147.67.11.6]) 11, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uB7EGFNo015200 11, 29 -- for {Microscopy-at-Microscopy.com} ; Wed, 7 Dec 2016 08:16:16 -0600 11, 29 -- Received: from S-DC-EMP016-E.net1.cec.eu.int (158.167.3.15) by 11, 29 -- S-DC-EDG005-Z.rcnet.cec.eu.int (147.67.11.6) with Microsoft SMTP Server (TLS) 11, 29 -- id 14.3.301.0; Wed, 7 Dec 2016 15:18:34 +0100 11, 29 -- Received: from S-DC-EMP012-B.net1.cec.eu.int (158.167.2.38) by 11, 29 -- S-DC-EMP016-E.net1.cec.eu.int (158.167.3.15) with Microsoft SMTP Server (TLS) 11, 29 -- id 14.3.301.0; Wed, 7 Dec 2016 15:18:35 +0100 11, 29 -- Received: from S-DC-ESTE03-B.net1.cec.eu.int ([169.254.2.95]) by 11, 29 -- S-DC-EMP012-B.net1.cec.eu.int ([158.167.2.38]) with mapi id 14.03.0301.000; 11, 29 -- Wed, 7 Dec 2016 15:18:35 +0100 11, 29 -- From: {Philipp.POEML-at-ec.europa.eu} 11, 29 -- To: {Microscopy-at-Microscopy.com} 11, 29 -- Subject: EMAS 2017 and IUMAS-7 in Konstanz, Germany, May 7-11 2017 11, 29 -- Thread-Topic: EMAS 2017 and IUMAS-7 in Konstanz, Germany, May 7-11 2017 11, 29 -- Thread-Index: AdJQlGGpUi4Z/0wxTKy1duuF5Df4MQ== 11, 29 -- Date: Wed, 7 Dec 2016 14:18:34 +0000 11, 29 -- Message-ID: {42FA29275AD9F5459EC54F35C8CE04FD6AB20AE0-at-S-DC-ESTE03-B.net1.cec.eu.int} 11, 29 -- Accept-Language: en-GB, fr-LU, en-US 11, 29 -- Content-Language: en-US 11, 29 -- X-MS-Has-Attach: 11, 29 -- X-MS-TNEF-Correlator: 11, 29 -- x-originating-ip: [158.167.189.41] 11, 29 -- Content-Type: text/plain; charset="utf-8" 11, 29 -- MIME-Version: 1.0 11, 29 -- Content-Transfer-Encoding: 8bit 11, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id uB7EGFNo015200 ==============================End of - Headers==============================
DEPARTMENT OF HEALTH & HUMAN SERVICES Food and Drug Administration
Electron Microscopist Nanotechnology Core Facility National Center for Toxicological Research
The U.S. Department of Health and Human Services, Food and Drug Administration (FDA), National Center for Toxicological Research (NCTR), Nanotechnology Core Facility (NanoCore) is seeking to hire an experienced and motivated electron microscopist. The NanoCore has several advanced Transmission Electron Microscopes (TEM) and Scanning Electron Microscopes (SEM) to characterize nanomaterial and detect nanomaterials in cells and tissues, and analyze the structure of cells/tissues at the electron microscopic level. The equipment include 120 and 200 kV TEM, low voltage (25 kV) TEM, high resolution FE-SEM, and FE-SEM equipped with ultramicrotome for serial block face scanning electron microscopy (SBF-SEM). Applicants must have experience and proficiency in classical electron microscopy specimen preparation techniques including tissue fixation, dehydration, infiltration, embedding and ultramicrotomy. Experience with nanomaterial imaging, image analysis, electron diffraction, tomography, and energy-dispersive X-ray spectroscopy is desirable. Candidates are expected to have an interest and ability to learn and apply new techniques in order to become proficient in the cutting edge technologies employed in our facility. Additional duties will include organizing and maintaining the electron microscopy laboratory, ordering supplies, maintaining records and possess excellent written and oral communicating skills.
Job Qualifications: . Must have and demonstrate experience and proficiency in electron microscopy . Must possess a Bachelor or Master's degree in either material science, chemistry, or any biological field. . Experience with nanotechnology and or image analysis is desirable.
Salary for this position will be commensurate with experience. Interested individuals should send their resume to Dr. Anil K. Patri at the following address:
Anil K. Patri, Ph.D., Director, NCTR-ORA Nanocore HFT-30, 3900 NCTR Road, Jefferson, AR 72079 anil.patri-at-fda.hhs.gov
Applications will be accepted until the position is filled. FDA is an Equal Opportunity Employer. FDA/NCTR is a smoke free environment.
The NCTR, located approximately 30 miles southeast of Little Rock, Arkansas, conducts FDA mission-related research that is of critical importance to the Agency in developing a scientifically sound basis for regulatory decisions. Over 150 Ph.D. scientists and 400 support scientists, on-site-contractors and administrative staff make up a dynamic group of professionals in the NCTR organization. Undergraduate and graduate students, post-doctoral fellows and visiting scientists also pursue education and research opportunities in a multi- disciplinary team atmosphere. For more information on NCTR research and training activities, visit http://www.fda.gov/NCTR.
==============================Original Headers============================== 14, 37 -- From Angel.Paredes-at-fda.hhs.gov Wed Dec 7 12:52:43 2016 14, 37 -- Received: from fda-ifw-00.fda.gov (fda-ifw-00.fda.gov [150.148.0.65]) 14, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uB7IqhoQ018917 14, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Dec 2016 12:52:43 -0600 14, 37 -- X-SBRS: None 14, 37 -- X-MID: 85477473 14, 37 -- X-IronPort-Anti-Spam-Filtered: true 14, 37 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2FABABiWkhY/yoQqApeHQEFAQsBgzkBA?= 14, 37 -- =?us-ascii?q?QEBAWsOgQAGB7sVQyWFfQKCPBEBAgEBAQEBAQEDfAuCWj0KMgEBAQEBAQEBAQE?= 14, 37 -- =?us-ascii?q?BAQEBARoCIS8BARwDXywBKlYmAQQTCA4GiFyqVIs2AQsBJIY/hCiEbw2DL4IwB?= 14, 37 -- =?us-ascii?q?Y89iyQGAYFJiBSXfYlviCQ1gRuFdHIBiBKBDQEBAQ?= 14, 37 -- X-IPAS-Result: =?us-ascii?q?A2FABABiWkhY/yoQqApeHQEFAQsBgzkBAQEBAWsOgQAGB7s?= 14, 37 -- =?us-ascii?q?VQyWFfQKCPBEBAgEBAQEBAQEDfAuCWj0KMgEBAQEBAQEBAQEBAQEBARoCIS8BA?= 14, 37 -- =?us-ascii?q?RwDXywBKlYmAQQTCA4GiFyqVIs2AQsBJIY/hCiEbw2DL4IwBY89iyQGAYFJiBS?= 14, 37 -- =?us-ascii?q?XfYlviCQ1gRuFdHIBiBKBDQEBAQ?= 14, 37 -- X-IronPort-AV: E=Sophos;i="5.33,315,1477972800"; 14, 37 -- d="scan'208";a="85477473" 14, 37 -- Received: from fdswp09439.fda.gov ([10.168.16.42]) 14, 37 -- by fda-ifw-00.fda.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 07 Dec 2016 13:55:25 -0500 14, 37 -- Received: from FDSWP09431.fda.gov ([169.254.14.10]) by FDSWP09439.fda.gov 14, 37 -- ([10.168.16.42]) with mapi id 14.03.0319.002; Wed, 7 Dec 2016 13:55:25 -0500 14, 37 -- From: "Paredes, Angel" {Angel.Paredes-at-fda.hhs.gov} 14, 37 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 14, 37 -- Subject: Position available 14, 37 -- Thread-Topic: Position available 14, 37 -- Thread-Index: AdJQu3Yf5KFtzBTqQoGzR3o7sm43wg== 14, 37 -- Date: Wed, 7 Dec 2016 18:55:24 +0000 14, 37 -- Message-ID: {0762EB2AD3E84740915AAF4A66BA841361F7DE75-at-FDSWP09431.fda.gov} 14, 37 -- Accept-Language: en-US 14, 37 -- Content-Language: en-US 14, 37 -- X-MS-Has-Attach: 14, 37 -- X-MS-TNEF-Correlator: 14, 37 -- x-originating-ip: [10.168.18.7] 14, 37 -- Content-Type: text/plain; charset="iso-8859-1" 14, 37 -- MIME-Version: 1.0 14, 37 -- Content-Transfer-Encoding: 8bit 14, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uB7IqhoQ018917 ==============================End of - Headers==============================
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Email: degraef-at-cmu.edu Name: Marc De Graef
Organization: Carnegie Mellon University
Title-Subject: [Filtered] Foundations of Electron Microscopy Short Course
Message: I would like to draw your attention to a Short Course offered early January (10-11 2017) at Johns Hopkins University in Baltimore. The course will cover theory and simulations for imaging and diffraction in both SEM and TEM and will be at the graduate level. Course and registration information can be found at the following URL: http://hemi.jhu.edu/academic-programs/short-courses/foundations-of-electron-microscopy/
Please pass this on to any graduate students or post-doctoral researchers who may be interested in this course.
Regards, Marc.
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I'd like to draw your attention to a new tool for microscopists I've developed for Android devices, "Resolution".
After entering magnification, immersion medium, lambda, and NA the App will calculate resolution (actual and theoretical), axial resolution and fluorescence brightness of your objective.
Each objective entered can be easily saved and restored.
You can select your camera, binning and additional magnification to determine if you're sampling at Nyquist frequency.
All information generated get be easily shared via e-mail or MMS with the share button.
The App is free to download and compatible with phones or tablets running Android 4.0 or above.
I hope you find it useful!
Andrew L. Barlow
Please use the link below on your Android device to install:
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Email: ciasem2017-at-imre.uh.cu Name: Carlos Alberto Lariot
Organization: Cuban Society of Microscopy
Title-Subject: [Filtered] Announcement of CIASEM 2017
Message: XIV Interamerican Congress on Microscopy SECOND ANNOUNCEMENT The Cuban Society of Microscopy and the Organizing Committee of the XIV Interamerican Congress on Microscopy (CIASEM 2017) announces some important aspects of this conference, which will be held next year, from September 25 to 29 in Varadero, the most beautiful beach of Cuba. CIASEM 2017 will include eight symposia in Material Science, seven in Life Sciences, one in Advances in Instrumentation and one in Teaching of Morphological Sciences. Five Precongress courses updating on various topics of microscopy and a micrograph contest are also scheduled. There will be a Commercial Exhibition of companies that supply microscopes, as well as other equipment and reagents for microscopy. A profitable interchange with their skilled specialists may represent a valuable update for all the delegates regarding new equipment and analytical techniques. Students and early career researchers can apply for the scholarships that the International Federation of Microscopy (IFSM) and the Microscopy Society of America (MSA) will provide to attend CIASEM 2017. A special package which includes a 5-night, all-inclusive accommodation at the Melia Marina Varadero Hotel 5* (venue of this conference) with a customized assistance after arrival to Cuba at Havana or Varadero Airport, is provided for the CIASEM 2017 delegates, and those flying by COPA Airlines will have a 20% discount. Besides the charm of the beautiful Varadero beach, and the comfortable, welcoming Melia hotel, visitors will have the opportunity to go on excursions to several tourist attractions of our country. In our website, www.ciasem2017.sld.cu, you can log in and find updated information during all the organization stages of CIASEM 2017. Login Host: 200.55.139.211 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: jingyue.liu-at-asu.edu Name: Jingyue (Jimmy) Liu
Organization: Arizona State University
Title-Subject: [Filtered] Postdoc in AC-STEM Immediately at ASU
Message: Immediately Available A Postdoctoral Research Associate position in aberration-corrected STEM of supported metal catalysts is immediately available in Professor Jingyue (Jimmy) Liu's group at Arizona State University (ASU). Responsibilities: The Postdoctoral Research Associate is responsible for characterizing a variety of supported metal/alloy nanoparticle catalysts, analyzing and evaluating the results, correlating the structure-performance relationships, discussing data with multi-site project team members, and writing technical reports and manuscripts for peer reviewed journal publications. Qualifications: A Ph.D. in chemistry, materials science and engineering, physics or a related field; outstanding knowledge and skills in AC-STEM and associated techniques (HAADF, EDS and EELS); extensive experience in STEM characterization of supported metal/alloy catalysts; knowledge and experience in using modern SEM, microprobe and XRD.
To apply, please submit a single PDF document containing (1) a cover letter which includes the names and email addresses of three references and (2) a curriculum vitae with a list of publications to Jingyue.liu-at-asu.edu.
Note: This position is funded by an industry partner and is initially planned for two years depending on performance and available funding. ASU will provide H-1B visa for all Postdoctoral Research Associates.
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Email: 13qw9-at-queensu.ca Name: Jason
Title-Subject: [Filtered] Position changes when change sample height
Message: Hello SEM users, I have a problem about using an FEI SEM. After I focused and linked Z, the sample position will always change a lot when I raise or drop the sample. Basically the area in the image will go to the opposite direction with the sample.why would this happen? Seems like this only happens in my acciu. I don't have much knowledge about SEM theory so, any advice is appreciated.
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Title-Subject: [Filtered] Embedding of nerves for TEM
Message: I have tried to embed nerves in LRWhite 3 times now, but it is not successful. The last time I washed the samples more than usual, change the diluted and 100 % embedding media more than twice as often, and spend 5 days on the procedure. The nerves are all soft, I get an empty space under the sample, and they are impossible to section. I thought the problem was getting rid of all the osmium, because I embedded some without osmium, and they turned out fine. Does anyone know what the problem could be, and how I should solve it? Best regards Lene
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} On Dec 11, 2016, at 9:17 AM, microscopy.listserver-at-gmail.com wrote: } } } X-from: 13qw9-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } Email: 13qw9-at-queensu.ca Name: Jason } } Title-Subject: [Filtered] Position changes when change sample height } } Message: Hello SEM users, } I have a problem about using an FEI SEM. After I focused and linked Z, the sample position will } always change a lot when I raise or drop the sample. Basically the area in the image will go to the } opposite direction with the sample.why would this happen? Seems like this only happens in my acciu. } I don't have much knowledge about SEM theory so, any advice is appreciated. } Dear Jason, It seems like the beam is tilted. Have you done that alignment? I am not an expert on SEM, so maybe someone who is will also reply. Yours, Bill
==============================Original Headers============================== 6, 34 -- From wtivol-at-sbcglobal.net Mon Dec 12 18:20:38 2016 6, 34 -- Received: from nm19-vm2.access.bullet.mail.bf1.yahoo.com (nm19-vm2.access.bullet.mail.bf1.yahoo.com [216.109.115.97]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uBD0KbgO008825 6, 34 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2016 18:20:38 -0600 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1481588618; bh=NQq5cNPAUeRd7u03kI9kyJpUp89G1iRdV/TPd++ZKSE=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=exTrBTbOxDTfoR4vHoISUTA2UAOQrZxlgbsN3dKV/gj56NFRfmwVRnnqivRFw9WnvvPvVsvlaRhnPAZufzKhgRFTY0g22T8rInCeSzIkygmZJZBH8JoeTojnPpJAHTjjZbxi0IEZmU7T2Y89cF1Xd4FjVGNh9zYWZvvaU38HIeE/JkmcsixcboznoAgmwJU5IiXpYMUA9nF+j/7E0kZsEpvsOKynImHr67X2OtS+yfmvruXj2Cv2Wk2PMNJkDT+yLdejRsozftUkhIwrE1quVOJsImwFmdEuGHzgER9gBFieO4ybwQGHmWysO9bsKu+25ViUqEYbhBaairOIKE6Asg== 6, 34 -- Received: from [66.196.81.158] by nm19.access.bullet.mail.bf1.yahoo.com with NNFMP; 13 Dec 2016 00:23:38 -0000 6, 34 -- Received: from [98.139.221.157] by tm4.access.bullet.mail.bf1.yahoo.com with NNFMP; 13 Dec 2016 00:23:38 -0000 6, 34 -- Received: from [127.0.0.1] by smtp117.sbc.mail.bf1.yahoo.com with NNFMP; 13 Dec 2016 00:23:38 -0000 6, 34 -- X-Yahoo-Newman-Id: 831212.96498.bm-at-smtp117.sbc.mail.bf1.yahoo.com 6, 34 -- X-Yahoo-Newman-Property: ymail-3 6, 34 -- X-YMail-OSG: NwPUKoYVM1nxuY6G7YBAzUh3n4_3zXR9pYAJh3JvVeqnOQp 6, 34 -- 4j4lGteb5DCqgFqbnklhauraFTJIercx0WmIy1i3kEr3wK.16gASEuZn3V1K 6, 34 -- P5C4eUG4wNuUcoD_lgsGPa2yvC2zmaMAjhdSq8w0fV9roLv0a.gFkz1J.nnY 6, 34 -- fFRLztVT2Voc49qqCVfAi0MwEyX4oSS0xtcHSW0iYk7buue2ZpM98ULkcjXH 6, 34 -- uUi0KuKuLocaOxUyFoN7fDp3lUyx8W1ldseX0SOHUcWOnf1t2i0edK4bPALV 6, 34 -- WTEOtluZoyhpUjIL5kJ1VgEhFOkRBl41.oM2CdFqFNx8a8kqe7OyfRSwUgCy 6, 34 -- OV7sPWNHJo5Ico3b96eKJvdji1mkU9jDJ7QDP1fefYU3n9PI52PND7wXXqbl 6, 34 -- 75Z2Qa_M5Ra6kvciDdgt4d8y1W.n3kLcW168Jg1dHNmT1yVaiV4xVQuJ4C6C 6, 34 -- MvLAkEYuXUAUmr9qX0__yR0JoLQAjYMzbmvkJj1A9R4fpv_v1RRMOSrZOBHL 6, 34 -- CW_h2ujmDVuffY4ptI06iu3tUL9v0ZVIivQuPw6uzDAFVHobvN9aftiiPnZY 6, 34 -- dbRU- 6, 34 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 34 -- Content-Type: text/plain; charset=us-ascii 6, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 6, 34 -- Subject: Re: [Microscopy] viaWWW: SEM - Position changes when change sample height 6, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 34 -- In-Reply-To: {201612111717.uBBHHCLB022222-at-ns.microscopy.com} 6, 34 -- Date: Mon, 12 Dec 2016 16:23:34 -0800 6, 34 -- Message-Id: {C7BA6892-BDFE-4986-AB64-30843B0A16F2-at-sbcglobal.net} 6, 34 -- References: {201612111717.uBBHHCLB022222-at-ns.microscopy.com} 6, 34 -- To: microscopy-at-microscopy.com, 13qw9-at-queensu.ca 6, 34 -- X-Mailer: Apple Mail (2.3124) 6, 34 -- Content-Transfer-Encoding: 8bit 6, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uBD0KbgO008825 ==============================End of - Headers==============================
It it sounds like there is quite a bit of movement in the image. If so, it may be that the stage Z axis isn't parallel to the beam axis.
First determine in which direction the image is shifting. On most of the FEI scopes I've worked with, the stage Y axis is vertical and X axis is horizontal on the screen. If the image shift is along the vertical (Y) axis, adjust your sample tilt several degrees + & -. One direction should increase the shift and the other decrease it. If the shift is along the X axis, visually examine the stage from the side and see if you can see anything that is cocked.
I would recommend discretion when fiddling with the stage since the bearings and other components are matched for submicron precision. This may be worth a service call.
Good luck, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
When you say change or drop the specimen I assume you mean a Z position adjustment? The "problem" that you see with a change in Z is quite normal. The vertical motion of the stage is through a thread and the take up of any slack in that thread results in specimen movement in X and Y directions. With an electrically driven stage manufacturers have the option of providing anti backlash adjustments to make X and Y movements more positive; I do not know a manufacturer that has anti backlash compensation in the Z direction.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
Title-Subject: [Filtered] Position changes when change sample height
Message: Hello SEM users, I have a problem about using an FEI SEM. After I focused and linked Z, the sample position will always change a lot when I raise or drop the sample. Basically the area in the image will go to the opposite direction with the sample.why would this happen? Seems like this only happens in my acciu. I don't have much knowledge about SEM theory so, any advice is appreciated.
==============================Original Headers============================== 10, 20 -- From protrain-at-emcourses.com Tue Dec 13 04:45:36 2016 10, 20 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uBDAjZhJ011793 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2016 04:45:35 -0600 10, 20 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 10, 20 -- id {B584fd1fc0000} ; Tue, 13 Dec 2016 10:48:28 +0000 10, 20 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Tue, 13 Dec 2016 10:48:27 +0000 10, 20 -- From: "Steve Chapman" {protrain-at-emcourses.com} 10, 20 -- To: {microscopy-at-microscopy.com} 10, 20 -- Cc: {13qw9-at-queensu.ca} 10, 20 -- Subject: SEM - Position changes when change sample height 10, 20 -- Date: Tue, 13 Dec 2016 10:48:03 -0000 10, 20 -- Message-ID: {004401d2552e$6544f400$2fcedc00$-at-com} 10, 20 -- MIME-Version: 1.0 10, 20 -- Content-Type: text/plain; 10, 20 -- charset="us-ascii" 10, 20 -- Content-Transfer-Encoding: 7bit 10, 20 -- X-Mailer: Microsoft Office Outlook 12.0 10, 20 -- Thread-Index: AdJT0MKUIEsIeEqNTOmp+Y9kEva9ywAkG8EwADMv5BA= 10, 20 -- Content-Language: en-gb ==============================End of - Headers==============================
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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis
Organization: USDA-ARS
Title-Subject: [Filtered] particle separation on microscope slide
Message: Hello folks- I have an upcoming project to measure the particle sizes of powders/grindings. Do folks have a best practice on getting good separation of particles on a microscope slide? Samples will be wet.
If anyone has a clever idea on how to do this it would be much appreciated.
Thanks
Joe
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Email: crozier-at-asu.edu Name: Peter Crozier
Organization: Arizona State University
Title-Subject: [Filtered] EDGE EELS Meeting in Japan
Message: The next International Electron Energy Loss Spectroscopy Meeting (EDGE 2017), will be held at Okuma, Okinawa, Japan, May 14 19, 2017. The purpose of the meeting is to cover the latest developments in electron energy loss spectroscopy and related techniques in the transmission electron microscope. The event is attended by approximately 100-130 participants from around the world in an informal setting and is aimed at promoting scientific discussions and cross-fertilization of ideas from complementary techniques. Abstracts will be due January 22, 2017. More information can be found in the attached flyer and at the conference website at: http://www.nims.go.jp/EDGE2017/.
Best Regards, Scientific and Local Host Committee Chairs Odile Stéphan, Peter Crozier and Koji Kimoto,
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Email: fitzp-at-wustl.edu Name: James Fitzpatrick
Organization: Washington University School of Medicine, St. Louis, MO
Title-Subject: [Filtered] Job Opening - Electron Microscopy Specialist
Message: I am hiring an Electron Microscopy Specialist for my lab, the Center for Cellular Imaging at Washington University School of Medicine - http://wucci.wustl.edu.
Ideal candidate will have a background in Cell Biology, with thin section and freeze fracture expertise along with high-resolution TEM analysis. Apply to Job ID: 35418 - Research Specialist (Electron Microscopy) - Center for Cellular Imaging
https://jobs.wustl.edu
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Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] Carbon Grains on Formvar Grids
Message: What is the approximate size of the carbon grains on Formvar grids? I hava also noted that if my objective aperture on the TEM is out, the grains seem to disappear (if that is what they are) when I image at 60-90kx. I am guessing that the "rough", salt-and-pepper appearance on the grids is carbon. I am using a TEM designed for biological samples. Thanks, Vickie
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} Email: vakimler-at-oakland.edu Name: Vickie Kimler } } Organization: Oakland University Eye Research Institute } } Title-Subject: [Filtered] Carbon Grains on Formvar Grids } } Message: What is the approximate size of the carbon grains on Formvar grids? I hava also noted that } if my objective aperture on the TEM is out, the grains seem to disappear (if that is what they are) } when I image at 60-90kx. I am guessing that the "rough", salt-and-pepper appearance on the grids is } carbon. I am using a TEM designed for biological samples. Thanks, Vickie
Dear Vickie, Amorphous carbon has no long-range order, but as the carbon atoms are deposited on the formvar, they form graphite-like bonds. There are probably patches of these bonded carbon atoms about 1 nm across—this is just a guess. The image loses contrast when you remove the objective aperture, because all the scattered electrons contribute to the image, which is mostly phase contrast. With the aperture in place, some of the scattered electrons are removed from the image, and these are the ones scattered by smaller objects, such as carbon grains. Furthermore, the closer you are to exact focus, the lower the contrast. If you see rough grains, they are due to defocus fringes that overlap, and if you look at the Fourier transform, you will see Thon rings. You can use these to correct astigmatism, determine the resolution of the instrument, or determine the value of the defocus. It should not matter whether your scope is for biology or materials. Yours, Bill
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Email: pveril-at-uth.gr Name: P. Berillis
Organization: University of Thessaly, Hellas
Title-Subject: [Filtered] CM10 vacuum problem
Message: Hi everybody. I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked fine and in high magnification (we examined some virus particles). When I finished I shut it down. Yesterday when I switch it on the microscope stacked to the "vacuum status: start up" for hours. I can hear the PVP working, but the pre-vacuum led is of and the ODP is cold. The monitor shows these pressure values: P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers? With my best regards Panos
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If your PVP is running with out shutting down you most likely have a vacuum leak in the backing lines.
Check the hoses from the roughing pump to the DP. Are they old and cracked? The other place to check is the o-ring connections on the DP backing line. They also get old (hot) and cracked. I had to replace them on my CM200 just a month ago.
Cheers,
Nestor Your Friendly Neighborhood SysOp
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Email: pveril-at-uth.gr Name: P. Berillis
Organization: University of Thessaly, Hellas
Title-Subject: [Filtered] CM10 vacuum problem
Message: Hi everybody. I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked fine and in high magnification (we examined some virus particles). When I finished I shut it down. Yesterday when I switch it on the microscope stacked to the "vacuum status: start up" for hours. I can hear the PVP working, but the pre-vacuum led is of and the ODP is cold. The monitor shows these pressure values: P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers? With my best regards Panos
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we have an older model (1995 or so) Aspex PSEM and are in general familiar with the electronics in the SEM. A few weeks ago, we noticed that the emission current started to jump around. This continued after replacing the filament, but stopped lateron and we are now stuck with a low emission current.
After filament alignment using the source imaging mode, we are now stuck with no more than 23uA of emission current at 20kV, the highest bias voltage setting and the standard 200um aperture in the liner tube. By turning up the "filament drive" software control, the emission current steadily increases until the 23uA at about "65%" filament drive. Turning up the filament drive more than that does no longer increase the emission current.
As I mentioned, we are familiar with the SEM electronics and we are willing to take things apart and have a look for faulty components including measurements on the PCBs. Since the emission current started to jump around before we got the low emission current, I would suspect that something in the high voltage power supply failed (maybe a faulty cap or something).
Before taking apart things, do you have any suggestions of what we could try to ramp up the emission current ? The filament should be aligned and the bias voltage is already at its maximum.
Are there any specific electronic components in the HVPS that are known to cause this behavior if they fail (e.g. failing capacitors due to their age, voltage regulators) ?
Is there any information available on the HVPS test points and the voltages we should get there so that we can compare them to the voltages within our system ?
Hi Panos, Edwards ODP has Oil Fill and Oil Drain connections on base of pump. Each item has O ring seal. Area subject to very high temperature results in deform and cracking of seals. Replace with Kalrez type O rings. Confirmation is with Prevac Pump running, readout of P1 and P2 does not fall below 38 as indicated.
Regards, Dave. eV Optics cc South Africa.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 22 December 2016 03:55 PM To: evoptics-at-iafrica.com
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Email: pveril-at-uth.gr Name: P. Berillis
Organization: University of Thessaly, Hellas
Title-Subject: [Filtered] CM10 vacuum problem
Message: Hi everybody. I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked fine and in high magnification (we examined some virus particles). When I finished I shut it down. Yesterday when I switch it on the microscope stacked to the "vacuum status: start up" for hours. I can hear the PVP working, but the pre-vacuum led is of and the ODP is cold. The monitor shows these pressure values: P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers? With my best regards Panos
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possibly vacuum leak anywhere between PVP and ODP Especially if PVP stays ON continuously. Likely places PVP inlet hose and ODP dipstick O-rings. The doubt is in P1/P2 = 38. Anything below 39 should not prevent ODP from heating up.
possibly PVP is not performing, possibly just needs oil change.
possibly ODP heater is bad or has no power. The doubt is - this shouldn't prevent P1/P2 from reaching values much lower than 38.
assuming P1/P2 readings correct (they are unlikely to be equally incorrect).
I need more details for remote diagnosis, you welcome to contact me off list.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
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Vickie, Bill; The apparent carbon “grains” you see are purely an artifact of imaging in a microscope with a lot of spherical aberration! The Thon Rings you see in your FFT are a rotational display of the square of the modulation transfer function of the objective lens, which changes as focus changes. If all this is a mystery to you, you should attend the ASU Winter School on High-Resolution Electron Microscopy, which starts January 3rd. If you cannot do that, read the classic (and superb) text ’Experimental High-Resolution Electron Microscopy” by John Spence.
A. John Mardinly, Ph.D., P.E. Retired Principal Materials Nanoanalysis Engineer ASU
} On Dec 21, 2016, at 6:05 PM, wtivol-at-sbcglobal.net wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DQIBaQ&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=DK633RWYCDDFlVrngHv5eR19LB37l8ce2UVM48Lxo-s&s=vT6_Zf5diTMx6eicnLsr1Q6fOxlaQv12GxXkm6669uU&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DQIBaQ&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=DK633RWYCDDFlVrngHv5eR19LB37l8ce2UVM48Lxo-s&s=WKv5mAF1FoBrwI5Vl9H4TJgUg4JiEj9VvJ9xW__groQ&e= } ---------------------------------------------------------------------------- } } } Email: vakimler-at-oakland.edu Name: Vickie Kimler } } } } Organization: Oakland University Eye Research Institute } } } } Title-Subject: [Filtered] Carbon Grains on Formvar Grids } } } } Message: What is the approximate size of the carbon grains on Formvar grids? I hava also noted that } } if my objective aperture on the TEM is out, the grains seem to disappear (if that is what they are) } } when I image at 60-90kx. I am guessing that the "rough", salt-and-pepper appearance on the grids is } } carbon. I am using a TEM designed for biological samples. Thanks, Vickie } } Dear Vickie, } Amorphous carbon has no long-range order, but as the carbon atoms are deposited on the formvar, they form graphite-like bonds. There are probably patches of these bonded carbon atoms about 1 nm across—this is just a guess. The image loses contrast when you remove the objective aperture, because all the scattered electrons contribute to the image, which is mostly phase contrast. With the aperture in place, some of the scattered electrons are removed from the image, and these are the ones scattered by smaller objects, such as carbon grains. Furthermore, the closer you are to exact focus, the lower the contrast. If you see rough grains, they are due to defocus fringes that overlap, and if you look at the Fourier transform, you will see Thon rings. You can use these to correct astigmatism, determine the resolution of the instrument, or determine the value of the defocus. It should not matter whether your scope is for biology or materials. } Yours, } Bill } } } } } } ==============================Original Headers============================== } 6, 32 -- From wtivol-at-sbcglobal.net Wed Dec 21 18:46:20 2016 } 6, 32 -- Received: from nm1-vm8.access.bullet.mail.bf1.yahoo.com (nm1-vm8.access.bullet.mail.bf1.yahoo.com [216.109.114.79]) } 6, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id uBM0kKxW003872 } 6, 32 -- for {microscopy-at-microscopy.com} ; Wed, 21 Dec 2016 18:46:20 -0600 } 6, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1482367794; bh=+Bkw2fgNckf2R0cW+YD/8c6pQzuQboq8BlSsSK37CoM=; h=From:Subject:Date:To:From:Subject; b=YjFDNTY+Qs40JJGWoipoODg1K6w+TBi4eTFt7JQ5UQK2L7VNnZy5719SqubVJXGUgZzXMbFu863NIwf1jG5xIzhXqMTLeB/7GkVBp8hbBTkZ4t/j2BC/2+/gW3TkXOL1RLrl3Gi2cZ45+JML928AfZ8n/VIfImXNSUSFCp4sY/pHqGe3HzYbFqtFQeIEdfYgrNgl3GH5rAZQSYXXoLuOarm3n9P3zySgC/rktnWi3kIDEw6aZyBzfAKRiq+7xfu7dN8oJ5WLSvGmXUVAE4aKSGC7+1mVNkvT5acUsRmYv8LuGU4EsawHaZZ9hUMUVtwLj4+AGDMO+8a976h19u3q4w== } 6, 32 -- Received: from [66.196.81.157] by nm1.access.bullet.mail.bf1.yahoo.com with NNFMP; 22 Dec 2016 00:49:54 -0000 } 6, 32 -- Received: from [98.139.221.157] by tm3.access.bullet.mail.bf1.yahoo.com with NNFMP; 22 Dec 2016 00:49:54 -0000 } 6, 32 -- Received: from [127.0.0.1] by smtp117.sbc.mail.bf1.yahoo.com with NNFMP; 22 Dec 2016 00:49:54 -0000 } 6, 32 -- X-Yahoo-Newman-Id: 351442.31272.bm-at-smtp117.sbc.mail.bf1.yahoo.com } 6, 32 -- X-Yahoo-Newman-Property: ymail-3 } 6, 32 -- X-YMail-OSG: S0teCTcVM1npbLtcIxTNicfW1_6EtM2I_X79w8LJxGm8XDQ } 6, 32 -- xNWFYYeX7s1blpbLGDPjomLjvJYYG0z3nNUKwigEmVKm4q8uwjff5vlowaSc } 6, 32 -- gMrLBo5HPMDma8IO2Wn85t.fQmFYpkZc4YEj6g771u0ZCyT6H9IWxzvOx1VI } 6, 32 -- bE.eaEi5J9q0N6y0T_ErIy6z2mjwDYEFeclUk0j0z_rsMrcACPRKOIUjjKlh } 6, 32 -- .H_foFq5pEgaq7bQofxODkfYTKr6cAGnBjsYcctlZsRsjd07lqo03nIu2VLX } 6, 32 -- 6be.tfCFUVqviqQC2H1z4gjBl48r34m7RcXifb2MAd1wcPOTFsm7mPRM9k4t } 6, 32 -- TVbZO.qN2qCcKRoyt27en1Lh.qDTaWpsfe85oggreAn2O98gGgZmDo0DwIJ5 } 6, 32 -- lvPCbqwbRrtlMIH1aqi_SYbChdSfHFedvxMsuZ3jiSUbExjxcTASEZxXYBvZ } 6, 32 -- XhtUPEWO3MI8EY1JskplCoDaiEfomXnnCZNY7GyKyYDRsWy4PVMbBafRWRNo } 6, 32 -- j786tfNsEV3kq7fxLM1qs4VjBV.il9bO4GXP9HbAqI2ouTTtZo53yEzrq4vb } 6, 32 -- aciG6eR0- } 6, 32 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t } 6, 32 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} } 6, 32 -- Content-Type: text/plain; charset=utf-8 } 6, 32 -- Subject: Carbon Grains on Formvar Grids } 6, 32 -- Message-Id: {CE8EE960-414D-4BCB-A429-1385C3A20FBD-at-sbcglobal.net} } 6, 32 -- Date: Wed, 21 Dec 2016 16:49:51 -0800 } 6, 32 -- To: vakimler-at-oakland.edu, microscopy-at-microscopy.com } 6, 32 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) } 6, 32 -- X-Mailer: Apple Mail (2.3124) } 6, 32 -- Content-Transfer-Encoding: 8bit } 6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id uBM0kKxW003872 } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 64 -- From John.Mardinly-at-asu.edu Thu Dec 22 13:31:22 2016 12, 64 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 12, 64 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id uBMJVMBR020591 12, 64 -- for {Microscopy-at-Microscopy.com} ; Thu, 22 Dec 2016 13:31:22 -0600 12, 64 -- X-ASG-Debug-ID: 1482435292-05202e068e01cd0006-FOsErg 12, 64 -- Received: from ppnetw4.asu.edu (ppnetw4.asu.edu [10.118.52.71]) by bcnet6.asu.edu with ESMTP id pIKA49NbIcoOpp4Z (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Thu, 22 Dec 2016 12:34:55 -0700 (MST) 12, 64 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 12, 64 -- Received: from pps.filterd (ppnetw4.asu.edu [127.0.0.1]) 12, 64 -- by ppnetw4.asu.edu (8.16.0.17/8.16.0.17) with SMTP id uBMJKt1v014238; 12, 64 -- Thu, 22 Dec 2016 12:23:37 -0700 12, 64 -- Received: from exhubt04.asurite.ad.asu.edu (exnswvpx-10-118-52-65.inre.asu.edu [10.118.52.65]) 12, 64 -- by ppnetw4.asu.edu with ESMTP id 27g22rh51v-1 12, 64 -- (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NOT); 12, 64 -- Thu, 22 Dec 2016 12:23:37 -0700 12, 64 -- Received: from EXMBT01.asurite.ad.asu.edu ([129.219.103.44]) by 12, 64 -- exhubt04.asurite.ad.asu.edu ([129.219.103.43]) with mapi id 14.03.0266.001; 12, 64 -- Thu, 22 Dec 2016 12:23:36 -0700 12, 64 -- From: John Mardinly {John.Mardinly-at-asu.edu} 12, 64 -- X-Barracuda-Effective-Source-IP: exmbt01.asurite.ad.asu.edu[129.219.103.44] 12, 64 -- X-Barracuda-Apparent-Source-IP: 129.219.103.44 12, 64 -- To: "wtivol-at-sbcglobal.net" {wtivol-at-sbcglobal.net} , 12, 64 -- MSA 12, 64 -- {Microscopy-at-Microscopy.com} 12, 64 -- Subject: Re: [Microscopy] Carbon Grains on Formvar Grids 12, 64 -- Thread-Topic: [Microscopy] Carbon Grains on Formvar Grids 12, 64 -- X-ASG-Orig-Subj: Re: [Microscopy] Carbon Grains on Formvar Grids 12, 64 -- Thread-Index: AQHSW+/uRLKbg3cclkGgbVvLGj+JZKEUzz+A 12, 64 -- Date: Thu, 22 Dec 2016 19:23:35 +0000 12, 64 -- Message-ID: {27D6E24E-B6E2-4B08-BE31-7DC5E327F106-at-asu.edu} 12, 64 -- References: {201612220105.uBM151KE016987-at-ns.microscopy.com} 12, 64 -- In-Reply-To: {201612220105.uBM151KE016987-at-ns.microscopy.com} 12, 64 -- Accept-Language: en-US 12, 64 -- Content-Language: en-US 12, 64 -- X-MS-Has-Attach: 12, 64 -- X-MS-TNEF-Correlator: 12, 64 -- x-originating-ip: [129.219.103.4] 12, 64 -- Content-Type: text/plain; charset="utf-8" 12, 64 -- Content-ID: {902D0F49A11ABC4F815DD55CCEDA22E0-at-exchange.asu.edu} 12, 64 -- MIME-Version: 1.0 12, 64 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2016-12-22_15:,, 12, 64 -- signatures=0 12, 64 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 12, 64 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 12, 64 -- adjust=0 reason=mlx scancount=1 engine=8.0.1-1612050000 12, 64 -- definitions=main-1612220311 12, 64 -- X-Barracuda-Connect: ppnetw4.asu.edu[10.118.52.71] 12, 64 -- X-Barracuda-Start-Time: 1482435295 12, 64 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 12, 64 -- X-Barracuda-URL: https://129.219.117.248:443/cgi-mod/mark.cgi 12, 64 -- X-Barracuda-Scan-Msg-Size: 6220 12, 64 -- X-Virus-Scanned: by bsmtpd at asu.edu 12, 64 -- X-Barracuda-BRTS-Status: 1 12, 64 -- X-Barracuda-Spam-Score: 0.23 12, 64 -- X-Barracuda-Spam-Status: No, SCORE=0.23 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.0 tests=BSF_SC0_MISMATCH_TO, SH_BIG5_05413_BODY_104, THREAD_INDEX, THREAD_TOPIC 12, 64 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.35326 12, 64 -- Rule breakdown below 12, 64 -- pts rule name description 12, 64 -- ---- ---------------------- -------------------------------------------------- 12, 64 -- 0.01 THREAD_INDEX thread-index: AcO7Y8iR61tzADqsRmmc5wNiFHEOig== 12, 64 -- 0.01 THREAD_TOPIC Thread-Topic: ...(Japanese Subject)... 12, 64 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header 12, 64 -- 0.21 SH_BIG5_05413_BODY_104 BODY: Body: contain "UNSUBSCRIBE" 12, 64 -- Content-Transfer-Encoding: 8bit 12, 64 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id uBMJVMBR020591 ==============================End of - Headers==============================
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Email: researchers4u-at-gmail.com Name: Sun
Organization: Shiraz university, iran
Title-Subject: [Filtered] SEM Peltier cooling stage
Message: Dear colleagues: recently we are trying to purchase a peltier cooling stage for the SEM samples that needs to be frozen like: milk,ice cream, fruits and generally food industry. And this stage that has been offered to us by a middle company gets to -50 centigrade to +70 centigrade. I was wondering for our applications is this temperature range is enough? Or should we go for other cooling stages with -180 centigrade?
Thank you for your information and time
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Email: susan.wert-at-cchmc.org Name: Susan E. Wert, PhD
Organization: Cincinnati Children's Hospital Medical Center/Research Foundation
Title-Subject: [Filtered] TEM Drierite drying columns
Message: I have 2 unused Drierite drying columns 2 5/8 x 11 3/8 inches, molded acrylic plastic, with anodized aluminum caps fitted with O-ring gaskets, for working pressures up to 90 psig. Connections for rubber tubing at top and bottom of column, at right angles to the tube. Flow rate 200 liters per hour. Still in original boxes purchased for a Balzers freeze-fracture machine. Age unknown, but Drierite is pink and would need to be regenerated. Free except for cost of shipping. Otherwise would appreciate ideas for recycling.
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As you consider the cooling stage, you should think about the vapor pressure of what you are inserting into your chamber. I assume that you have a high-vacuum SEM and that the primary vapor you are concerned with is water. The tables of water vapor pressure as a function of temperature should be readily available online. You want to ensure that the partial pressure of water is *much* below (orders of magnitude) the chamber pressure of your scope.
Also, you need to take into account the thermal conductivity of the sample you are examining. Remember that the temperature of the exposed surface can be well above the cooling temperature of the stage itself. Remember that the beam will also cause local heating of the sample. In my TEM, I have seen ice evaporate under the beam when the stage temperature was reading {-100C.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 12/23/2016 7:31:48 AM
First, to all of you vendors and labs that I have no dealings with except for being on this list, and who are still dutifully going to tell me that you are out of the office for the holidays, I wish you a Merry Christmas and Happy New Year anyway. I do still wish that you would find a way to spare list-posters from your notices.
I will second Henk's comments that the beam will lead to localized increased temperatures. You will have to play around with the conditions to get what you want.
We have a Peltier stage on our Quanta. I'm not sure that ours has as much as a -50C to 70C range. I think our cooler allows 25C deviation up or down from ambient and that is relative to a temperature conditioned water bath. I am not sure you would need the -180C model.
I would ask you to consider the full process of your experiments. How will you introduce your samples? Do you have a cryo transfer system? How will you prepare your samples for examination? That could be in-situ but probably elsewhere. Do you have an SEM capable of low-vacuum or environmental mode?
Henk, you spoke of keeping the vapor pressure of the water in the sample far below the pressure of the chamber. Are you dealing with a high-vacuum-only instrument? Couldn't that still result in problems? If the partial pressure of water in your vacuum is below the vapor pressure of water at the sample temperature, the water in the sample would still sublime. Granted, if the temperature is very low, that should be a slow process. Conversely, if the partial pressure of water in your chamber is higher that the vapor pressure of water at sample temperature, then you would have frost build up on the sample through deposition. It will be a tricky business.
Warren
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Friday, December 23, 2016 8:42 AM To: Straszheim, Warren E [BIOTC]
Hi Sun,
As you consider the cooling stage, you should think about the vapor pressure of what you are inserting into your chamber. I assume that you have a high-vacuum SEM and that the primary vapor you are concerned with is water. The tables of water vapor pressure as a function of temperature should be readily available online. You want to ensure that the partial pressure of water is *much* below (orders of magnitude) the chamber pressure of your scope.
Also, you need to take into account the thermal conductivity of the sample you are examining. Remember that the temperature of the exposed surface can be well above the cooling temperature of the stage itself. Remember that the beam will also cause local heating of the sample. In my TEM, I have seen ice evaporate under the beam when the stage temperature was reading {-100C.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 12/23/2016 7:31:48 AM
This response will be obscure, because I learned my skills from instructors who learned in the early 20th century.
1. When I covered a section on a 1x3" slide, I normally used a 7/8 x 7/8" #1 cover-slip. 2. The mountant was prepared "in house" from "Dewaxed Gum Damar" 3. When I used up what I was given with my PhD, I purchased a 1 lb jar of dewaxed chunks of Gum Damar a. De-waxed was NOT de-junked, so I dissolved the lb in a gallon of xylene so I could filter the solution to remove all of the 'other' undissolved stuff. As I recall, I performed this operation, in a hood, and did it at least twice. b. Then, I placed a cotton plug in the gallon container that was covered with a 250ml beaker and left with an appropriate sign - "to evaporate-to-appropriate consistency." [This was determined by the rates at which drops fell from the 1/8" glass applicator.) This took at least two years until I had a liter that has now sat for almost 20 years with only one aliquot removed. 4. Gum Damar was used, (anecdotally and empirically) because it did NOT dry out if applied at a proper concentration. I have slides that I prepared in the mid-1960's, and the appearance and clarity beneath the cover-slip has not changed. 5. How was I challenged to administer this wonderful material? a. Each applicant was advixsed to make a list of the number(s) of drops from the glass-rod applicator when the drops fell at a 'certain' rate, for each of the cover-slips that we had available in the lab - little circles to the cover-slips for 2x3" slides. 6. All the above now given as memory permits, I will give the routine when applying the cover-slip - my way. [I do not know how a modern applicator does this job, so I am unaware how it might be incorrectly done in the case of the ?!?"!?!?] a. Taking an individually cleaned - to biochemical standards! - cover-slip, place it on a clean 90mm circle of Whatman #2 - no ash , thus, absent of nano junk - and apply the required drops for the cover-slip being used in a personalized array of droplets. b. Bring the slide with a section out of its last 'zylene' rinse and wipe carefully on the back of the slide and around the section. When the breeze from the nearby open window has evaporated a sufficient amount of residual zylene, invert the slide and carefully - holding it at an appropriate angle - bring the slide to the mountant on the cover-slip and carefully bring the angle to zero trapping no bubbles in the process. c. Turning the slide right-side-up , set it aside in an appropriate tray, protected from the dust in the breeze. d. When all slide are prepared, having used 22x22mm #1 glass to cover the section on the 1x3" slide, place on each cover-slip a block of lead whose dimensions were 2-3mm less around its perimeter so as to avoid getting stuck to the cover-slip in the cases of new cover-slippers. i. This peculiar dimensional confusion was explained as an attempt to overcome the the slide we biologists had taken into the metric system while preparing for our careers in biology in chemistry departments across the planet.] ii. The purpose of the Pb was to minimize the mountant; for, the thickness of the section varies more than anyone knows - or, perhaps, cares. iii. Lots of new findings have found their way into journals when the explanations of the 'data' were the thickness of the sections rather than what was claimed. Two classes of Sertoli Cells in one case based on two classes of nuclei - 'dark' and 'light.' 7. So, as you may have discerned, I have a 'Monkish' manner about things scientific. a. Example 1: I do not ever want the mountant to dry. Rather, I would choose a mountant that on concentrating at its perimeter of the cover-slip such that it forms a seal that prevents any further drying. b. Example 2: I have NEVER taught 'di-section.' Rather, I have made use of my knowledge of proper prefixes and dutifully taught 'dis-section.' I once did a di-section before a class - with a machete - on a short shark in order to demonstrate the difference between what the students all said they were going to do and what I was going to grade them on! 8. AN UNIMPORTANT POSTLUDE. For those of you who chuckle with me about various things, I will leave you with a mystery that I will take to my grave. Background aside, there is no way that I should have received money from NIH (in the mid 1990's) to answer a simple histological question concerning the existence of a very rich capillary bed lying beneath the so-called impermeable epithelium that lines the mammalian urinary bladder. Not only for a proposal that was primarily anatomic but, to add to the question, a proposal including an experiment that was very simple to be nice. Yet, I/we received a lot of money based on a recommend of full acceptance from the peer review! Once we were found out by the folks from upstairs at NIH, however, the word came down that in order to get even consideration for an extension, I would have to apply the new methods of "Molecular Biology" to our problem. Thus, I outfitted an appropriate lab, and began to educate myself in a methodology that was based on an instrument called a "PCR." In the process, I discovered the truth that the primers were the key to success, and no one would share her/his method with anyone else. Time and pure luck paid off to my best skill - that I have always called, "LIBRARY!" Once in a satisfactory place, and with new-found confidence in the primary generator I had found, I received a visit from a colleague who had been recommended to me for help with his primer problem. Always one to share my knowledge, I spent about 90 minutes rustling up a set of primers that were perfect for his target using the program "BLAST" from NIH. The outcome of my contribution was ultimately a surprise report of a new human gene called, "TERE1" then, and "UBIAD1", now. In context, I would have appeared last in my class - at any level - as one likely to do research at the University of Pennsylvania, and more recently that I, an anatomist/histologist, would EVER have found himself associated with a gene on the human genome. Thus, like the axiom -"you can'! t win if you fail to play" - 'if you are not given the opportunity to discover, there is little chance that you will discover - and even then, you likely must be a team player.'
Finally, there is little magic in science that doesn't rise from experience, but in life, the magic may be common, plentiful, and wonderful.
God bless, and Happy New Year - which ever you follow,
Fred Monson
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu ________________________________________ X-from: Ifeoluwa Ajayi via Histonet {histonet-at-lists.utsouthwestern.edu} Sent: Thursday, December 22, 2016 12:08 AM To: Jessica Riggleman Cc: histonet-at-lists.utsouthwestern.edu
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